Rh6AG394300

3-ketoacyl-CoA synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0003748
Physical Location & Seq
Reverse (-)
59251434 .. 59254629
3196 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG394300.1

Sequence Viewer

Length: 1539 bp
ATGTCAAGGTCCAATTTAAATGCACCTTTAATATCTCCATCACTATCATCACCAAAGCTCCCTGATTTCAAACAATCTGTGAAATTGAAGTATGTAAAGCTTGGTTACCATTACCTCATCACTCATGGAATGTACTTGTTCCTAACCCCTCTTTTTGTTCTCACTGCTGCACAACTCTCCACATTCTCACTCCAAGACCTCCATGACCTTTGGGATCATTTGAGGTTCAATCTCATATCCGTTCTGCTCTGCTCAACTCTCTTGGTCTTCTTGTCCACCCTCTACTTCCTCACTCGTCCCCGCCCCGTTTACCTTGTTGATTTCTCATGCTACAAGCCCGAAGATGCTCGAAAAACCACTAGGCACGTGTTCATGAATTGCTCACATTCCATAGGAACATTCACTGGGGAGAATCTTGAGTTTCAGCGCAAAATTATTGAGAGGTCAGGTCTTGGGGAGTCTACATATCTCCCTGAAGCTGTTATAAGGGTGCCTCCTAATCCATGTATGGCAGAGGCAAGAAAAGAAGCTGAGATGGTAATGTATGGTGCCCTTGATCAGCTGTTTGAGAAAACTTTTATAAAACCAAAGGACATTGGCATTTTGATTGTGAATTGTAGCTTGTTCAATCCAACACCATCTTTGTCAGCTATGGTGATCAATCATTATAAGCTACGAGGGAATGTTATTAGTTACAATCTTGGAGGGATGGGATGTAGTGCTGGTTTGATCTCGATTGATCTTGCTAAAGATCTTCTTCAAGTCCACCCGAATACCTATGCTTTGGTCATTAGTATGGAGAACATCACTTTGAATTGGTATTTTGGGAATGACAGGTCAATGCTTGTGTCAAACTGCTTGTTTCGAATGGGGGGAGCTGCAATTTTGCTTTCGAACAATAGGTCTGATAGGCGTCGATCCAAGTACCAGTTAGTCCATACTGTTCGAACACATAAAGGTGCTGATGACAAGTGTTTCTCTTGTGTTACTCAAAAGGAGGATCCTGCAGGGAAAATCGGTGTTTCTTTGTCAAAAGATCTTATGGCAGTAGCTGGGGATGCTTTGAAGACCAACATTACAACTTTGGGTCCTTTGGTGTTACCTATGTCTGAACAGTTGCTCTTCTTTGCTACACTTATTGGAAGAAAGCTTTTTAATATGAAGGTGAAGCCTTACATCCCGGATTTCAAATTGGCTTTTGAACATTTCTGCATTCATGCTGGAGGAAGAGCTGTGCTGGATGAATTGGAAAAGAATTTGCAGCTTTCAGACAGACATATGGAGCCTTCAAGGATGACCCTATACAGATTTGGCAACACCTCAAGCAGCTCTCTTTGGTATGAACTGGCTTATACAGAAGCCAAGGGAAGGATGAAGAAAGGTGACAGAACGTGGCAGATAGCTTTCGGGTCTGGCTTCAAGTGTAATAGCGCGGTTTGGAAGGCTTTGAGGACTGTGAATCCAGTAAAGGAGAAGAACCCGTGGATGGATGAGATCGACATGTTCCCGGTGCATGTTCCAAAGGTCTCAGCCCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006950 GO:0008150 GO:0009266 GO:0009314 GO:0009409 GO:0009416 GO:0009628 GO:0050896
KEGG Pathways
Metabolic & Signaling
ko00062 ko01110 map00062 map01110
Pfam Domains
Protein Families
ACP_syn_III_C FAE1_CUT1_RppA

Protein Analysis

512

Amino Acids

57.8

Weight (kDa)

9.25

Isoelectric Point (pI)

37.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAE1_CUT1_RppA PF08392 95 - 384 2.5e-144 FAE1/Type III polyketide synthase-like protein
ACP_syn_III_C PF08541 401 - 481 8.9e-10 3-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III C terminal
Chal_sti_synt_C PF02797 403 - 479 1.8e-09 Chalcone and stilbene synthases, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 485, 581, 669
AccB1I GGYRCC 2 cut(s) 490, 548
AccI GTMKAC 1 cut(s) 461
AccII CGCG 1 cut(s) 1433
AciI CCGC 2 cut(s) 301, 1433
AclWI GGATC 4 cut(s) 222, 912, 995, 1008
AcsI RAATTY 1 cut(s) 1255
AcuI CTGAAG 1 cut(s) 495
AcvI CACGTG 1 cut(s) 367
AcyI GRCGYC 1 cut(s) 913
AfaI GTAC 2 cut(s) 134, 926
AfiI CCNNNNNNNGG 2 cut(s) 1368, 1486
AflIII ACRYGT 2 cut(s) 366, 1500
Alw26I GTCTC 1 cut(s) 1531
AlwI GGATC 4 cut(s) 222, 912, 995, 1008
AlwNI CAGNNNCTG 1 cut(s) 1052
ApeKI GCWGC 4 cut(s) 167, 878, 1261, 1326
ApoI RAATTY 1 cut(s) 1255
ArsI GACNNNNNNTTYG 2 cut(s) 887, 919
AspLEI GCGC 2 cut(s) 429, 1433
AspS9I GGNCC 2 cut(s) 9, 1088
AsuC2I CCSGG 2 cut(s) 1181, 1508
AsuHPI GGTGA 4 cut(s) 42, 667, 1177, 1394
AsuII TTCGAA 3 cut(s) 865, 893, 946
AvaII GGWCC 2 cut(s) 9, 1088
BaeGI GKGCMC 1 cut(s) 553
BamHI GGATCC 1 cut(s) 1000
BanI GGYRCC 2 cut(s) 490, 548
BbrPI CACGTG 1 cut(s) 367
BbsI GAAGAC 2 cut(s) 259, 1073
BbvI GCAGC 4 cut(s) 154, 865, 1273, 1338
BccI CCATC 5 cut(s) 46, 529, 646, 703, 1480
BclI TGATCA 2 cut(s) 556, 657
BcnI CCSGG 2 cut(s) 1181, 1508
BcoDI GTCTC 1 cut(s) 1531
BfaI CTAG 1 cut(s) 360
BfmI CTRYAG 1 cut(s) 1005
BglII AGATCT 2 cut(s) 751, 1036
BisI GCNGC 4 cut(s) 168, 879, 1262, 1327
BlsI GCNGC 4 cut(s) 169, 880, 1263, 1328
Bme1390I CCNGG 2 cut(s) 1181, 1508
Bme18I GGWCC 2 cut(s) 9, 1088
BmgT120I GGNCC 2 cut(s) 9, 1088
BmiI GGNNCC 5 cut(s) 492, 550, 1002, 1089, 1284
BmrFI CCNGG 2 cut(s) 1181, 1508
BmrI ACTGGG 1 cut(s) 414
BmsI GCATC 2 cut(s) 334, 1048
BmuI ACTGGG 1 cut(s) 414
BpiI GAAGAC 2 cut(s) 259, 1073
BpmI CTGGAG 1 cut(s) 1242
Bpu14I TTCGAA 3 cut(s) 865, 893, 946
BpuEI CTTGAG 2 cut(s) 437, 1306
BpuMI CCSGG 2 cut(s) 1181, 1508
BsaAI YACGTR 1 cut(s) 367
BsaHI GRCGYC 1 cut(s) 913
BsaI GGTCTC 1 cut(s) 1531
BsaJI CCNNGG 2 cut(s) 1362, 1481
BsaXI ACNNNNNCTCC 2 cut(s) 42, 72
Bsc4I CCNNNNNNNGG 2 cut(s) 1368, 1486
Bse1I ACTGG 4 cut(s) 409, 928, 1350, 1463
BseDI CCNNGG 2 cut(s) 1362, 1481
BseGI GGATG 9 cut(s) 714, 719, 1063, 1176, 1246, 1299, 1377, 1491, 1495
BseLI CCNNNNNNNGG 2 cut(s) 1368, 1486
BseMII CTCAG 1 cut(s) 522
BseNI ACTGG 4 cut(s) 409, 928, 1350, 1463
BseSI GKGCMC 1 cut(s) 553
BseXI GCAGC 4 cut(s) 154, 865, 1273, 1338
BseYI CCCAGC 1 cut(s) 1052
BsgI GTGCAG 1 cut(s) 153
Bsh1236I CGCG 1 cut(s) 1433
BshNI GGYRCC 2 cut(s) 490, 548
BsiSI CCGG 2 cut(s) 1181, 1508
BslFI GGGAC 1 cut(s) 282
BslI CCNNNNNNNGG 2 cut(s) 1368, 1486
BsmAI GTCTC 1 cut(s) 1531
BsmFI GGGAC 1 cut(s) 282
BsmI GAATGC 1 cut(s) 1212
Bso31I GGTCTC 1 cut(s) 1531
Bsp119I TTCGAA 3 cut(s) 865, 893, 946
Bsp1286I GDGCHC 1 cut(s) 553
BspACI CCGC 2 cut(s) 301, 1433
BspCNI CTCAG 1 cut(s) 523
BspFNI CGCG 1 cut(s) 1433
BspHI TCATGA 1 cut(s) 372
BspLI GGNNCC 5 cut(s) 492, 550, 1002, 1089, 1284
BspMAI CTGCAG 1 cut(s) 1009
BspPI GGATC 4 cut(s) 222, 912, 995, 1008
BspQI GCTCTTC 2 cut(s) 1127, 1222
BspT104I TTCGAA 3 cut(s) 865, 893, 946
BspT107I GGYRCC 2 cut(s) 490, 548
BspTNI GGTCTC 1 cut(s) 1531
BsrI ACTGG 4 cut(s) 409, 928, 1350, 1463
BssECI CCNNGG 2 cut(s) 1362, 1481
BssNI GRCGYC 1 cut(s) 913
BssT1I CCWWGG 1 cut(s) 1362
Bst4CI ACNGT 3 cut(s) 943, 1116, 1456
Bst6I CTCTTC 2 cut(s) 1127, 1222
BstACI GRCGYC 1 cut(s) 913
BstBAI YACGTR 1 cut(s) 367
BstBI TTCGAA 3 cut(s) 865, 893, 946
BstDEI CTNAG 2 cut(s) 531, 1528
BstDSI CCRYGG 1 cut(s) 1481
BstEII GGTNACC 1 cut(s) 104
BstF5I GGATG 9 cut(s) 714, 719, 1063, 1176, 1246, 1299, 1377, 1491, 1495
BstFNI CGCG 1 cut(s) 1433
BstHHI GCGC 2 cut(s) 429, 1433
BstMAI GTCTC 1 cut(s) 1531
BstMWI GCNNNNNNNGC 1 cut(s) 1058
BstNSI RCATGY 2 cut(s) 1504, 1517
BstPI GGTNACC 1 cut(s) 104
BstSCI CCNGG 2 cut(s) 1179, 1506
BstSFI CTRYAG 1 cut(s) 1005
BstSLI GKGCMC 1 cut(s) 553
BstUI CGCG 1 cut(s) 1433
BstV1I GCAGC 4 cut(s) 154, 865, 1273, 1338
BstV2I GAAGAC 2 cut(s) 259, 1073
BstX2I RGATCY 3 cut(s) 751, 1000, 1036
BstYI RGATCY 3 cut(s) 751, 1000, 1036
BtgI CCRYGG 1 cut(s) 1481
BtsCI GGATG 9 cut(s) 714, 719, 1063, 1176, 1246, 1299, 1377, 1491, 1495
BtsI GCAGTG 1 cut(s) 162
BtsIMutI CAGTG 2 cut(s) 162, 402
CaiI CAGNNNCTG 1 cut(s) 1052
CciI TCATGA 1 cut(s) 372
CfoI GCGC 2 cut(s) 429, 1433
Cfr13I GGNCC 2 cut(s) 9, 1088
CseI GACGC 1 cut(s) 902
Csp6I GTAC 2 cut(s) 133, 925
CviAII CATG 8 cut(s) 125, 203, 327, 373, 504, 1217, 1501, 1514
CviQI GTAC 2 cut(s) 133, 925
DdeI CTNAG 2 cut(s) 531, 1528
DraI TTTAAA 1 cut(s) 18
Eam1104I CTCTTC 2 cut(s) 1127, 1222
EarI CTCTTC 2 cut(s) 1127, 1222
Eco130I CCWWGG 1 cut(s) 1362
Eco31I GGTCTC 1 cut(s) 1531
Eco47I GGWCC 2 cut(s) 9, 1088
Eco57I CTGAAG 1 cut(s) 495
Eco72I CACGTG 1 cut(s) 367
Eco91I GGTNACC 1 cut(s) 104
EcoO109I RGGNCCY 1 cut(s) 1088
EcoO65I GGTNACC 1 cut(s) 104
EcoT14I CCWWGG 1 cut(s) 1362
ErhI CCWWGG 1 cut(s) 1362
FaeI CATG 8 cut(s) 128, 206, 330, 376, 507, 1220, 1504, 1517
FalI AAGNNNNNCTT 2 cut(s) 741, 773
FaqI GGGAC 1 cut(s) 282
FatI CATG 8 cut(s) 124, 202, 326, 372, 503, 1216, 1500, 1513
FauI CCCGC 1 cut(s) 308
FauNDI CATATG 1 cut(s) 1278
FbaI TGATCA 2 cut(s) 556, 657
FblI GTMKAC 1 cut(s) 461
Fnu4HI GCNGC 4 cut(s) 168, 879, 1262, 1327
FokI GGATG 9 cut(s) 721, 726, 1070, 1163, 1253, 1306, 1384, 1498, 1502
Fsp4HI GCNGC 4 cut(s) 168, 879, 1262, 1327
FspBI CTAG 1 cut(s) 360
GlaI GCGC 2 cut(s) 428, 1432
GluI GCNGC 4 cut(s) 168, 879, 1262, 1327
GsaI CCCAGC 1 cut(s) 1056
GsuI CTGGAG 1 cut(s) 1242
HapII CCGG 2 cut(s) 1181, 1508
HgaI GACGC 1 cut(s) 902
HhaI GCGC 2 cut(s) 429, 1433
Hin1I GRCGYC 1 cut(s) 913
Hin1II CATG 8 cut(s) 128, 206, 330, 376, 507, 1220, 1504, 1517
Hin6I GCGC 2 cut(s) 427, 1431
HinP1I GCGC 2 cut(s) 427, 1431
HindIII AAGCTT 2 cut(s) 98, 1148
HinfI GANTC 3 cut(s) 412, 458, 1459
HpaII CCGG 2 cut(s) 1181, 1508
HphI GGTGA 4 cut(s) 42, 667, 1177, 1394
Hpy166II GTNNAC 4 cut(s) 276, 310, 462, 766
Hpy188I TCNGA 3 cut(s) 907, 1111, 1270
Hpy188III TCNNGA 3 cut(s) 373, 416, 733
Hpy8I GTNNAC 4 cut(s) 276, 310, 462, 766
Hpy99I CGWCG 1 cut(s) 918
HpyAV CCTTC 4 cut(s) 1156, 1296, 1362, 1435
HpyCH4III ACNGT 3 cut(s) 943, 1116, 1456
HpyCH4IV ACGT 2 cut(s) 366, 1391
HpyCH4V TGCA 7 cut(s) 23, 170, 881, 1007, 1212, 1261, 1513
HpyF10VI GCNNNNNNNGC 1 cut(s) 1058
HpyF3I CTNAG 2 cut(s) 531, 1528
HpySE526I ACGT 2 cut(s) 366, 1391
Hsp92I GRCGYC 1 cut(s) 913
Hsp92II CATG 8 cut(s) 128, 206, 330, 376, 507, 1220, 1504, 1517
HspAI GCGC 2 cut(s) 427, 1431
Ksp22I TGATCA 2 cut(s) 556, 657
LguI GCTCTTC 2 cut(s) 1127, 1222
LmnI GCTCC 3 cut(s) 63, 875, 1282
Lsp1109I GCAGC 4 cut(s) 154, 865, 1273, 1338
LweI GCATC 2 cut(s) 334, 1048
MaeI CTAG 1 cut(s) 360
MaeII ACGT 2 cut(s) 366, 1391
MaeIII GTNAC 5 cut(s) 104, 692, 985, 1098, 1382
MflI RGATCY 3 cut(s) 751, 1000, 1036
MhlI GDGCHC 1 cut(s) 553
MlyI GAGTC 1 cut(s) 467
MmeI TCCRAC 1 cut(s) 656
MseI TTAA 3 cut(s) 17, 29, 1155
MslI CAYNNNNRTG 2 cut(s) 794, 957
MspA1I CMGCKG 1 cut(s) 562
MspI CCGG 2 cut(s) 1181, 1508
MspR9I CCNGG 2 cut(s) 1181, 1508
Mva1269I GAATGC 1 cut(s) 1212
MvnI CGCG 1 cut(s) 1433
MwoI GCNNNNNNNGC 1 cut(s) 1058
NciI CCSGG 2 cut(s) 1181, 1508
NdeI CATATG 1 cut(s) 1278
NlaIII CATG 8 cut(s) 128, 206, 330, 376, 507, 1220, 1504, 1517
NlaIV GGNNCC 5 cut(s) 492, 550, 1002, 1089, 1284
NmuCI GTSAC 1 cut(s) 1382
NspI RCATGY 2 cut(s) 1504, 1517
NspV TTCGAA 3 cut(s) 865, 893, 946
PagI TCATGA 1 cut(s) 372
PciI ACATGT 1 cut(s) 1500
PciSI GCTCTTC 2 cut(s) 1127, 1222
PctI GAATGC 1 cut(s) 1212
PfeI GAWTC 2 cut(s) 412, 1459
PfoI TCCNGGA 1 cut(s) 1179
PkrI GCNGC 4 cut(s) 169, 880, 1263, 1328
PleI GAGTC 1 cut(s) 466
PmaCI CACGTG 1 cut(s) 367
PmlI CACGTG 1 cut(s) 367
PpsI GAGTC 1 cut(s) 466
Ppu21I YACGTR 1 cut(s) 367
PpuMI RGGWCCY 1 cut(s) 1088
PscI ACATGT 1 cut(s) 1500
PsiI TTATAA 3 cut(s) 485, 581, 669
Psp5II RGGWCCY 1 cut(s) 1088
PspCI CACGTG 1 cut(s) 367
PspEI GGTNACC 1 cut(s) 104
PspFI CCCAGC 1 cut(s) 1052
PspN4I GGNNCC 5 cut(s) 492, 550, 1002, 1089, 1284
PspPI GGNCC 2 cut(s) 9, 1088
PspPPI RGGWCCY 1 cut(s) 1088
PstI CTGCAG 1 cut(s) 1009
PstNI CAGNNNCTG 1 cut(s) 1052
PsuI RGATCY 3 cut(s) 751, 1000, 1036
PvuII CAGCTG 1 cut(s) 562
RsaI GTAC 2 cut(s) 134, 926
RsaNI GTAC 2 cut(s) 133, 925
RseI CAYNNNNRTG 2 cut(s) 794, 957
SapI GCTCTTC 2 cut(s) 1127, 1222
SaqAI TTAA 3 cut(s) 17, 29, 1155
SatI GCNGC 4 cut(s) 168, 879, 1262, 1327
Sau96I GGNCC 2 cut(s) 9, 1088
SbfI CCTGCAGG 1 cut(s) 1009
SchI GAGTC 1 cut(s) 467
ScrFI CCNGG 2 cut(s) 1181, 1508
SdaI CCTGCAGG 1 cut(s) 1009
SduI GDGCHC 1 cut(s) 553
SfaNI GCATC 2 cut(s) 334, 1048
SfcI CTRYAG 1 cut(s) 1005
SfuI TTCGAA 3 cut(s) 865, 893, 946
SinI GGWCC 2 cut(s) 9, 1088
SmiI ATTTAAAT 1 cut(s) 18
SmiMI CAYNNNNRTG 2 cut(s) 794, 957
SmlI CTYRAG 2 cut(s) 416, 1321
SmoI CTYRAG 2 cut(s) 416, 1321
Sse8387I CCTGCAGG 1 cut(s) 1009
SsiI CCGC 2 cut(s) 301, 1433
SspMI CTAG 1 cut(s) 360
StyD4I CCNGG 2 cut(s) 1179, 1506
StyI CCWWGG 1 cut(s) 1362
SwaI ATTTAAAT 1 cut(s) 18
TaaI ACNGT 3 cut(s) 943, 1116, 1456
TaiI ACGT 2 cut(s) 369, 1394
TaqI TCGA 7 cut(s) 349, 734, 865, 893, 916, 946, 1497
TatI WGTACW 1 cut(s) 132
TfiI GAWTC 2 cut(s) 412, 1459
Tru1I TTAA 3 cut(s) 17, 29, 1155
Tru9I TTAA 3 cut(s) 17, 29, 1155
TscAI CASTG 2 cut(s) 169, 409
TseFI GTSAC 1 cut(s) 1382
TseI GCWGC 4 cut(s) 167, 878, 1261, 1326
Tsp45I GTSAC 1 cut(s) 1382
TspDTI ATGAA 7 cut(s) 361, 389, 1175, 1205, 1257, 1356, 1388
TspGWI ACGGA 1 cut(s) 229
TspRI CASTG 2 cut(s) 169, 409
VpaK11BI GGWCC 2 cut(s) 9, 1088
XapI RAATTY 1 cut(s) 1255
XceI RCATGY 2 cut(s) 1504, 1517
XmiI GTMKAC 1 cut(s) 461
XspI CTAG 1 cut(s) 360
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.