MD00G1025800.v1.1

3-ketoacyl-CoA synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0003748
Physical Location & Seq
Reverse (-)
4458227 .. 4461069
2843 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1025800.v1.1.491

Sequence Viewer

Length: 1560 bp
ATGGGAAAAATGGCAGACCCGAAACCCGACACACCCTTGATCCCACCATCGTCGTCATCACCATCGTCGTCAAGAAACCTCCCCGACTTCAAAAATTCAGTTAAACTGAAATATGTGAAGCTTGGGTACCATATTCTCATCACCCATGGCATGTACCTCTTTCTTTCCCCTCTTGTTGTCGTTATCGCTGCCCAGATTTCAACATTTTCTCTCAAGGATCTTTATGATCTTTGGGAGCATCTCCAGTATAACCTGATTTCAGTGATTATCTGCTCGACTCTCCTTGTTTTCCTATCCACCGTGTACTTTGTCACCCGCCCTCGCCCGGTGTACCTTGTTAACTTTTCGTGCTACAAGCCAGAAGAATCTCGAAAATGCACGAAAAAGATTTTCATGGAACAGTCTCAGATGACTGGTACTTTTACAGATGACAATCTTCAGTTCCAGCGTAGGATCCTTGAGAGATCTGGCCTTGGTGACTCGACTTACCTTCCCGAGGCAGTTCTCAACATTCCTCCAAATCCGTCAATGAAAGAAGCTAGAAAAGAAGCTGAGGCTGTGATGTTTGGTGCCATTGATGAGCTTTTCGCTAAGACAGCTGTTAAACCTAAGGACATTGGAATCTTGATTGTGAACTGCAGCTTGTTCAATCCAACCCCGTCTCTTTCTGCCATGGTTATCAATCATTACCAGCTTCGAGGGAACATAGTCAGCTATAATCTTGGTGGGATGGGATGCAGCGCAGGTCTGATCTCGATCGATCTTGCTAAAAATCTTCTTCAGGTGCATCCCAACTCCTATGCACTGGTTATCAGCATGGAGAACATCACCTTGAACTGGTACTTTGGAAACGACCGCTCAAAGCTTGTCTCAAATTGCTTATTCCGGATGGGAGGGGCTGCTATATTGCTTTCCAACAAAAGCTCAGACAAAAGAAGGTCCAAATACCGATTGGTTCATACTGTTCGCACTCACAAGGGTGCTGATGATAAATGCTTTAGCTGTGTTACCCAAGAAGAAGACGATAACGGGAAGATTGGTGTTACTTTATCAAAGGATCTGATGGCAGTAGCAGGTGATGCTCTAAAGACCAACATCACCACATTGGGGCCTCTTGTTTTGCCAATGTCCGAACAGCTGCTTTTCTTTGCGACATTGATTGGGAGGAAACTTTTCAAGATGAAGATCAAACCATACATCCCGGATTTCAGGCTAGCTTTTGAGCATTTCTGTATTCATGCTGGAGGGAGAGCGGTCTTGGATGAGTTGGAGAAGAACCTGCATCTATCGGATTGGCATATGGAGCCATCGAGGATGACACTCCACCGATTTGGAAACACCTCAAGTAGCTCTCTTTGGTATGAATTAGCTTACACAGAAGCCAAGGGAAGGATTAAGAAAGGAGATAGAACATGGCAGATAGCTTTCGGATCGGGATTCAAGTGCAACAGCGCTGTGTGGAAGGCTCTTAGGAGCATAAACCCAGCAAAGGAAAAGAACCCGTGGATGGATGAAATTGATCGGTTCCCAGTCGATGTTCCAAGGGTGTCAACCTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006950 GO:0008150 GO:0009266 GO:0009314 GO:0009409 GO:0009416 GO:0009628 GO:0050896
KEGG Pathways
Metabolic & Signaling
ko00062 ko01110 map00062 map01110
Pfam Domains
Protein Families
ACP_syn_III_C FAE1_CUT1_RppA

Protein Analysis

520

Amino Acids

58.4

Weight (kDa)

9.22

Isoelectric Point (pI)

41.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAE1_CUT1_RppA PF08392 102 - 391 8.3e-146 FAE1/Type III polyketide synthase-like protein
ACP_syn_III_C PF08541 408 - 488 1.5e-10 3-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III C terminal
Chal_sti_synt_C PF02797 410 - 486 1.2e-08 Chalcone and stilbene synthases, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1064
Acc36I ACCTGC 3 cut(s) 734, 1064, 1287
Acc65I GGTACC 1 cut(s) 126
AccB1I GGYRCC 2 cut(s) 126, 569
AccBSI CCGCTC 2 cut(s) 858, 1253
AccIII TCCGGA 1 cut(s) 885
AciI CCGC 3 cut(s) 316, 856, 1253
AclWI GGATC 6 cut(s) 34, 225, 448, 461, 1065, 1438
AcsI RAATTY 1 cut(s) 94
AcuI CTGAAG 2 cut(s) 422, 764
AfaI GTAC 6 cut(s) 128, 155, 305, 332, 418, 842
AfeI AGCGCT 1 cut(s) 1453
AfiI CCNNNNNNNGG 7 cut(s) 325, 496, 837, 1107, 1389, 1489, 1507
AgsI TTSAA 6 cut(s) 91, 201, 649, 835, 1177, 1441
AleI CACNNNNGTG 1 cut(s) 978
Alw26I GTCTC 3 cut(s) 408, 666, 874
AlwI GGATC 6 cut(s) 34, 225, 448, 461, 1065, 1438
Ama87I CYCGRG 1 cut(s) 494
Aor13HI TCCGGA 1 cut(s) 885
Aor51HI AGCGCT 1 cut(s) 1453
AoxI GGCC 2 cut(s) 469, 1109
ApeKI GCWGC 5 cut(s) 188, 639, 738, 899, 1138
ApoI RAATTY 1 cut(s) 94
Asp700I GAANNNNTTC 1 cut(s) 1172
Asp718I GGTACC 1 cut(s) 126
AspLEI GCGC 2 cut(s) 743, 1454
AspS9I GGNCC 2 cut(s) 939, 1109
AsuC2I CCSGG 2 cut(s) 326, 1202
AsuHPI GGTGA 7 cut(s) 51, 133, 304, 488, 820, 1088, 1090
AsuNHI GCTAGC 1 cut(s) 1213
AvaI CYCGRG 1 cut(s) 494
AvaII GGWCC 1 cut(s) 939
AxyI CCTNAGG 1 cut(s) 609
BamHI GGATCC 1 cut(s) 453
BanI GGYRCC 2 cut(s) 126, 569
BarI GAAGNNNNNNTAC 2 cut(s) 110, 142
BbsI GAAGAC 1 cut(s) 1026
BbvCI CCTCAGC 1 cut(s) 552
BbvI GCAGC 5 cut(s) 175, 651, 750, 886, 1125
BccI CCATC 7 cut(s) 55, 70, 724, 883, 1057, 1315, 1501
BcnI CCSGG 2 cut(s) 326, 1202
BcoDI GTCTC 3 cut(s) 408, 666, 874
BfaI CTAG 2 cut(s) 540, 1214
BfmI CTRYAG 1 cut(s) 637
BfoI RGCGCY 1 cut(s) 1455
BfuAI ACCTGC 3 cut(s) 734, 1064, 1287
BglII AGATCT 1 cut(s) 464
BisI GCNGC 5 cut(s) 189, 640, 739, 900, 1139
BlsI GCNGC 5 cut(s) 190, 641, 740, 901, 1140
Bme1390I CCNGG 2 cut(s) 326, 1202
Bme18I GGWCC 1 cut(s) 939
BmeT110I CYCGRG 1 cut(s) 494
BmgT120I GGNCC 2 cut(s) 939, 1109
BmiI GGNNCC 6 cut(s) 128, 455, 571, 1110, 1305, 1526
BmrFI CCNGG 2 cut(s) 326, 1202
BmrI ACTGGG 1 cut(s) 1523
BmsI GCATC 5 cut(s) 247, 725, 796, 1069, 1291
BmtI GCTAGC 1 cut(s) 1217
BmuI ACTGGG 1 cut(s) 1523
BpiI GAAGAC 1 cut(s) 1026
BpmI CTGGAG 2 cut(s) 227, 1263
Bpu10I CCTNAGC 1 cut(s) 552
BpuEI CTTGAG 3 cut(s) 197, 479, 1327
BpuMI CCSGG 2 cut(s) 326, 1202
Bsa29I ATCGAT 1 cut(s) 759
BsaBI GATNNNNATC 3 cut(s) 432, 755, 1184
BsaJI CCNNGG 7 cut(s) 145, 472, 495, 672, 1383, 1502, 1541
BsaWI WCCGGW 1 cut(s) 885
Bsc4I CCNNNNNNNGG 7 cut(s) 325, 496, 837, 1107, 1389, 1489, 1507
Bse1I ACTGG 5 cut(s) 244, 418, 810, 842, 1529
Bse21I CCTNAGG 1 cut(s) 609
Bse8I GATNNNNATC 3 cut(s) 432, 755, 1184
BseAI TCCGGA 1 cut(s) 885
BseCI ATCGAT 1 cut(s) 759
BseDI CCNNGG 7 cut(s) 145, 472, 495, 672, 1383, 1502, 1541
BseGI GGATG 9 cut(s) 735, 740, 787, 894, 1197, 1267, 1320, 1512, 1516
BseJI GATNNNNATC 3 cut(s) 432, 755, 1184
BseLI CCNNNNNNNGG 7 cut(s) 325, 496, 837, 1107, 1389, 1489, 1507
BseMII CTCAG 3 cut(s) 419, 543, 939
BseNI ACTGG 5 cut(s) 244, 418, 810, 842, 1529
BseXI GCAGC 5 cut(s) 175, 651, 750, 886, 1125
BseYI CCCAGC 1 cut(s) 1483
Bsh1285I CGRYCG 2 cut(s) 759, 856
BshFI GGCC 2 cut(s) 471, 1111
BshNI GGYRCC 2 cut(s) 126, 569
BshVI ATCGAT 1 cut(s) 759
BsiEI CGRYCG 2 cut(s) 759, 856
BsiHKCI CYCGRG 1 cut(s) 494
BsiSI CCGG 3 cut(s) 326, 886, 1202
BslI CCNNNNNNNGG 7 cut(s) 325, 496, 837, 1107, 1389, 1489, 1507
BsmAI GTCTC 3 cut(s) 408, 666, 874
BsmBI CGTCTC 1 cut(s) 666
BsnI GGCC 2 cut(s) 471, 1111
BsoBI CYCGRG 1 cut(s) 494
Bsp13I TCCGGA 1 cut(s) 885
Bsp19I CCATGG 2 cut(s) 145, 672
BspACI CCGC 3 cut(s) 316, 856, 1253
BspANI GGCC 2 cut(s) 471, 1111
BspCNI CTCAG 3 cut(s) 418, 544, 938
BspDI ATCGAT 1 cut(s) 759
BspEI TCCGGA 1 cut(s) 885
BspLI GGNNCC 6 cut(s) 128, 455, 571, 1110, 1305, 1526
BspMAI CTGCAG 1 cut(s) 641
BspMI ACCTGC 3 cut(s) 734, 1064, 1287
BspOI GCTAGC 1 cut(s) 1217
BspPI GGATC 6 cut(s) 34, 225, 448, 461, 1065, 1438
BspT107I GGYRCC 2 cut(s) 126, 569
BsrBI CCGCTC 2 cut(s) 858, 1253
BsrI ACTGG 5 cut(s) 244, 418, 810, 842, 1529
BssECI CCNNGG 7 cut(s) 145, 472, 495, 672, 1383, 1502, 1541
BssT1I CCWWGG 5 cut(s) 145, 472, 672, 1383, 1541
Bst4CI ACNGT 3 cut(s) 301, 402, 964
BstAPI GCANNNNNTGC 1 cut(s) 1079
BstC8I GCNNGC 1 cut(s) 1215
BstDEI CTNAG 6 cut(s) 405, 552, 591, 609, 925, 1469
BstDSI CCRYGG 3 cut(s) 145, 672, 1502
BstENI CCTNNNNNAGG 1 cut(s) 494
BstF5I GGATG 9 cut(s) 735, 740, 787, 894, 1197, 1267, 1320, 1512, 1516
BstH2I RGCGCY 1 cut(s) 1455
BstHHI GCGC 2 cut(s) 743, 1454
BstMAI GTCTC 3 cut(s) 408, 666, 874
BstMCI CGRYCG 2 cut(s) 759, 856
BstMWI GCNNNNNNNGC 3 cut(s) 596, 1079, 1303
BstNSI RCATGY 1 cut(s) 154
BstSCI CCNGG 2 cut(s) 324, 1200
BstSFI CTRYAG 1 cut(s) 637
BstV1I GCAGC 5 cut(s) 175, 651, 750, 886, 1125
BstV2I GAAGAC 1 cut(s) 1026
BstX2I RGATCY 4 cut(s) 217, 453, 464, 1057
BstXI CCANNNNNNTGG 1 cut(s) 1331
BstYI RGATCY 4 cut(s) 217, 453, 464, 1057
Bsu15I ATCGAT 1 cut(s) 759
Bsu36I CCTNAGG 1 cut(s) 609
BsuRI GGCC 2 cut(s) 471, 1111
BsuTUI ATCGAT 1 cut(s) 759
BtgI CCRYGG 3 cut(s) 145, 672, 1502
BtsCI GGATG 9 cut(s) 735, 740, 787, 894, 1197, 1267, 1320, 1512, 1516
BtsIMutI CAGTG 2 cut(s) 267, 803
BveI ACCTGC 3 cut(s) 734, 1064, 1287
Cac8I GCNNGC 1 cut(s) 1215
CfoI GCGC 2 cut(s) 743, 1454
Cfr13I GGNCC 2 cut(s) 939, 1109
ClaI ATCGAT 1 cut(s) 759
Csp6I GTAC 6 cut(s) 127, 154, 304, 331, 417, 841
CviAII CATG 7 cut(s) 146, 151, 394, 673, 817, 1238, 1413
CviQI GTAC 6 cut(s) 127, 154, 304, 331, 417, 841
DdeI CTNAG 6 cut(s) 405, 552, 591, 609, 925, 1469
Eco130I CCWWGG 5 cut(s) 145, 472, 672, 1383, 1541
Eco47I GGWCC 1 cut(s) 939
Eco47III AGCGCT 1 cut(s) 1453
Eco57I CTGAAG 2 cut(s) 422, 764
Eco81I CCTNAGG 1 cut(s) 609
Eco88I CYCGRG 1 cut(s) 494
EcoNI CCTNNNNNAGG 1 cut(s) 494
EcoO109I RGGNCCY 1 cut(s) 1109
EcoT14I CCWWGG 5 cut(s) 145, 472, 672, 1383, 1541
ErhI CCWWGG 5 cut(s) 145, 472, 672, 1383, 1541
Esp3I CGTCTC 1 cut(s) 666
FaeI CATG 7 cut(s) 149, 154, 397, 676, 820, 1241, 1416
FatI CATG 7 cut(s) 145, 150, 393, 672, 816, 1237, 1412
FauI CCCGC 1 cut(s) 323
FauNDI CATATG 1 cut(s) 1299
Fnu4HI GCNGC 5 cut(s) 189, 640, 739, 900, 1139
FokI GGATG 9 cut(s) 742, 747, 774, 901, 1184, 1274, 1327, 1519, 1523
Fsp4HI GCNGC 5 cut(s) 189, 640, 739, 900, 1139
FspBI CTAG 2 cut(s) 540, 1214
GlaI GCGC 2 cut(s) 742, 1453
GluI GCNGC 5 cut(s) 189, 640, 739, 900, 1139
GsaI CCCAGC 1 cut(s) 1487
GsuI CTGGAG 2 cut(s) 227, 1263
HaeII RGCGCY 1 cut(s) 1455
HaeIII GGCC 2 cut(s) 471, 1111
HapII CCGG 3 cut(s) 326, 886, 1202
HhaI GCGC 2 cut(s) 743, 1454
Hin1II CATG 7 cut(s) 149, 154, 397, 676, 820, 1241, 1416
Hin6I GCGC 2 cut(s) 741, 1452
HinP1I GCGC 2 cut(s) 741, 1452
HincII GTYRAC 2 cut(s) 340, 1551
HindII GTYRAC 2 cut(s) 340, 1551
HindIII AAGCTT 2 cut(s) 119, 863
HinfI GANTC 5 cut(s) 277, 365, 479, 621, 1437
HpaI GTTAAC 1 cut(s) 340
HpaII CCGG 3 cut(s) 326, 886, 1202
HphI GGTGA 7 cut(s) 51, 133, 304, 488, 820, 1088, 1090
Hpy166II GTNNAC 5 cut(s) 304, 331, 340, 634, 1551
Hpy188I TCNGA 7 cut(s) 408, 750, 928, 1062, 1132, 1291, 1430
Hpy188III TCNNGA 8 cut(s) 72, 369, 494, 625, 754, 886, 1177, 1434
Hpy8I GTNNAC 5 cut(s) 304, 331, 340, 634, 1551
Hpy99I CGWCG 2 cut(s) 55, 70
HpyAV CCTTC 4 cut(s) 500, 930, 1383, 1456
HpyCH4III ACNGT 3 cut(s) 301, 402, 964
HpyCH4V TGCA 7 cut(s) 378, 639, 738, 787, 803, 1282, 1446
HpyF10VI GCNNNNNNNGC 3 cut(s) 596, 1079, 1303
HpyF3I CTNAG 6 cut(s) 405, 552, 591, 609, 925, 1469
Hsp92II CATG 7 cut(s) 149, 154, 397, 676, 820, 1241, 1416
HspAI GCGC 2 cut(s) 741, 1452
Kpn2I TCCGGA 1 cut(s) 885
KpnI GGTACC 1 cut(s) 130
KspAI GTTAAC 1 cut(s) 340
LmnI GCTCC 3 cut(s) 235, 1303, 1473
Lsp1109I GCAGC 5 cut(s) 175, 651, 750, 886, 1125
LweI GCATC 5 cut(s) 247, 725, 796, 1069, 1291
MaeI CTAG 2 cut(s) 540, 1214
MaeIII GTNAC 4 cut(s) 310, 476, 1006, 1042
MbiI CCGCTC 2 cut(s) 858, 1253
MboII GAAGA 9 cut(s) 374, 428, 767, 770, 1028, 1031, 1045, 1195, 1285
MflI RGATCY 4 cut(s) 217, 453, 464, 1057
MluCI AATT 4 cut(s) 94, 874, 1364, 1515
MlyI GAGTC 2 cut(s) 271, 473
MmeI TCCRAC 3 cut(s) 677, 939, 1248
MroI TCCGGA 1 cut(s) 885
MroXI GAANNNNTTC 1 cut(s) 1172
MseI TTAA 4 cut(s) 102, 339, 603, 1395
MslI CAYNNNNRTG 1 cut(s) 978
MspA1I CMGCKG 2 cut(s) 599, 1138
MspI CCGG 3 cut(s) 326, 886, 1202
MspR9I CCNGG 2 cut(s) 326, 1202
MwoI GCNNNNNNNGC 3 cut(s) 596, 1079, 1303
NciI CCSGG 2 cut(s) 326, 1202
NcoI CCATGG 2 cut(s) 145, 672
NdeI CATATG 1 cut(s) 1299
NheI GCTAGC 1 cut(s) 1213
NlaIII CATG 7 cut(s) 149, 154, 397, 676, 820, 1241, 1416
NlaIV GGNNCC 6 cut(s) 128, 455, 571, 1110, 1305, 1526
NmuCI GTSAC 2 cut(s) 310, 476
NspI RCATGY 1 cut(s) 154
OliI CACNNNNGTG 1 cut(s) 978
PaqCI CACCTGC 1 cut(s) 1064
PdmI GAANNNNTTC 1 cut(s) 1172
PfeI GAWTC 3 cut(s) 365, 621, 1437
PfoI TCCNGGA 1 cut(s) 1200
PkrI GCNGC 5 cut(s) 190, 641, 740, 901, 1140
Ple19I CGATCG 1 cut(s) 759
PleI GAGTC 2 cut(s) 271, 473
PpsI GAGTC 2 cut(s) 271, 473
PspFI CCCAGC 1 cut(s) 1483
PspN4I GGNNCC 6 cut(s) 128, 455, 571, 1110, 1305, 1526
PspPI GGNCC 2 cut(s) 939, 1109
PstI CTGCAG 1 cut(s) 641
PsuI RGATCY 4 cut(s) 217, 453, 464, 1057
PvuI CGATCG 1 cut(s) 759
PvuII CAGCTG 2 cut(s) 599, 1138
RsaI GTAC 6 cut(s) 128, 155, 305, 332, 418, 842
RsaNI GTAC 6 cut(s) 127, 154, 304, 331, 417, 841
RseI CAYNNNNRTG 1 cut(s) 978
SaqAI TTAA 4 cut(s) 102, 339, 603, 1395
SatI GCNGC 5 cut(s) 189, 640, 739, 900, 1139
Sau96I GGNCC 2 cut(s) 939, 1109
SchI GAGTC 2 cut(s) 271, 473
ScrFI CCNGG 2 cut(s) 326, 1202
SfaNI GCATC 5 cut(s) 247, 725, 796, 1069, 1291
SfcI CTRYAG 1 cut(s) 637
SinI GGWCC 1 cut(s) 939
SmiMI CAYNNNNRTG 1 cut(s) 978
SmlI CTYRAG 3 cut(s) 212, 458, 1342
SmoI CTYRAG 3 cut(s) 212, 458, 1342
Sse9I AATT 4 cut(s) 94, 874, 1364, 1515
SsiI CCGC 3 cut(s) 316, 856, 1253
SspMI CTAG 2 cut(s) 540, 1214
StyD4I CCNGG 2 cut(s) 324, 1200
StyI CCWWGG 5 cut(s) 145, 472, 672, 1383, 1541
TaaI ACNGT 3 cut(s) 301, 402, 964
TaqI TCGA 8 cut(s) 275, 370, 482, 697, 755, 759, 1310, 1533
TasI AATT 4 cut(s) 94, 874, 1364, 1515
TatI WGTACW 1 cut(s) 303
TfiI GAWTC 3 cut(s) 365, 621, 1437
Tru1I TTAA 4 cut(s) 102, 339, 603, 1395
Tru9I TTAA 4 cut(s) 102, 339, 603, 1395
TscAI CASTG 2 cut(s) 267, 810
TseFI GTSAC 2 cut(s) 310, 476
TseI GCWGC 5 cut(s) 188, 639, 738, 899, 1138
Tsp45I GTSAC 2 cut(s) 310, 476
TspDTI ATGAA 7 cut(s) 382, 545, 947, 1196, 1226, 1377, 1527
TspGWI ACGGA 1 cut(s) 513
TspRI CASTG 2 cut(s) 267, 810
VpaK11BI GGWCC 1 cut(s) 939
XagI CCTNNNNNAGG 1 cut(s) 494
XapI RAATTY 1 cut(s) 94
XceI RCATGY 1 cut(s) 154
XcmI CCANNNNNNNNNTGG 1 cut(s) 949
XmnI GAANNNNTTC 1 cut(s) 1172
XspI CTAG 2 cut(s) 540, 1214
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.