FvH4_1g06010

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0008333
Physical Location & Seq
Reverse (-)
3211115 .. 3214324
3210 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g06010.t5

Sequence Viewer

Length: 1014 bp
ATGGAAAACGGAATCAACAACGGTGCTGGAGCCATAACAGTCTCGGCTCTCAATTATATTGATCTCTCAAGCACGGACACTCACCAATCCGTCTCTCTTCTCAAACAGGCCTGTTTGGATTGCGGTTTCTTCTACGTGGTAAATCATGGGATAAGTGAGAAATTCATGGACGAGGTGTTTTCACAGAGCAGGAGGATGTTCAGTTTACCGTTGAGTGAAAAGATGAAGCTTTTGAGGAACAAGAAGCACAGGGGTTATACTCCTGTTCTTGATGAGCATCTGGACCATGAAAACCAAGTTCATGTAGGAGATTACAAGGAGGGGTATTACATAGGAGTTGAGGTAGCTGAAGATGACCCAAAAGCGGAAAAGCCATTTTATGGTCCTAATGTTTGGCCTGCACCAGATATCTTACCTGGGTGGAGGGAGACCATGGAAGAATTTCATAGAGAAGCCTTAGAGGTGGCTAAAGCAGTTTCTAGGCTCATAGCCCTTGCTCTTGATCTAGATGTTCATTTCTTTGATAAGCAAGAAATGCTTGGTGAGGCTATTGCGACGTTGCGATTACTACACTATGAAGGAAAAATATCTGATCCATCAAATGGAATTTTCGGAGCGGGAGCTCATTCTGACTTCGGTCTCGTTACCCTCTTGGCAACCGATGATGTCTTAGGCCTCCAAATATGCAGGGATAAGGATGCCAAACCTCAGTTATGGGAATACGTACCGCCGGTTAAAGGAGCATTTATAGTGAATCTTGGCGACATGCTAGAACGCTGGAGCAACTGCATATTCAAGTCCACATTGCATAGAGTTGTTGGGAACGGTCAAGAGAGATATTCCATTGCATACTTCGTAGAACCTAGTCATGATTGTCTTGTGGAGTGCCTGCCTACGTGCAAATCAGAAAATAATCCTCCCAAGTTTCCTCCAATCTTATGTCGCACTTACCTGAGCCAGCGCTATAGTGATACTCATACCGATCTGACTAAATACGAAGAAATTAAGAAATAA

Protein Analysis

338

Amino Acids

38.13

Weight (kDa)

5.39

Isoelectric Point (pI)

36.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 18 - 133 4.3e-35 non-haem dioxygenase in morphine synthesis N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 380, 602
AccBSI CCGCTC 1 cut(s) 617
AciI CCGC 4 cut(s) 123, 365, 617, 728
AclWI GGATC 1 cut(s) 587
AcsI RAATTY 3 cut(s) 161, 440, 606
AcuI CTGAAG 1 cut(s) 369
AfaI GTAC 1 cut(s) 726
AfeI AGCGCT 1 cut(s) 962
AfiI CCNNNNNNNGG 4 cut(s) 364, 380, 602, 737
AgsI TTSAA 1 cut(s) 796
AjnI CCWGG 1 cut(s) 415
AluBI AGCT 3 cut(s) 229, 347, 623
AluI AGCT 3 cut(s) 229, 347, 623
Alw21I GWGCWC 1 cut(s) 625
Alw26I GTCTC 4 cut(s) 46, 97, 422, 644
AlwI GGATC 1 cut(s) 587
Aor51HI AGCGCT 1 cut(s) 962
AoxI GGCC 3 cut(s) 108, 395, 673
ApoI RAATTY 3 cut(s) 161, 440, 606
Asp700I GAANNNNTTC 1 cut(s) 441
AspLEI GCGC 1 cut(s) 963
AspS9I GGNCC 2 cut(s) 283, 383
AsuHPI GGTGA 2 cut(s) 74, 554
AvaII GGWCC 2 cut(s) 283, 383
BanII GRGCYC 1 cut(s) 625
Bbv12I GWGCWC 1 cut(s) 625
BccI CCATC 1 cut(s) 604
BciT130I CCWGG 1 cut(s) 417
BcoDI GTCTC 4 cut(s) 46, 97, 422, 644
BfaI CTAG 4 cut(s) 480, 506, 770, 864
BfmI CTRYAG 1 cut(s) 964
BfoI RGCGCY 1 cut(s) 964
Bme1390I CCNGG 1 cut(s) 417
Bme18I GGWCC 2 cut(s) 283, 383
BmgT120I GGNCC 2 cut(s) 283, 383
BmiI GGNNCC 1 cut(s) 31
BmrFI CCNGG 1 cut(s) 417
BmsI GCATC 2 cut(s) 286, 688
BoxI GACNNNNGTC 1 cut(s) 636
BpmI CTGGAG 2 cut(s) 48, 799
Bpu10I CCTNAGC 1 cut(s) 953
BpuEI CTTGAG 1 cut(s) 52
BsaAI YACGTR 3 cut(s) 136, 724, 897
BsaBI GATNNNNATC 1 cut(s) 276
BsaI GGTCTC 2 cut(s) 422, 644
BsaJI CCNNGG 2 cut(s) 416, 432
BsaXI ACNNNNNCTCC 2 cut(s) 327, 357
Bsc4I CCNNNNNNNGG 4 cut(s) 364, 380, 602, 737
Bse118I RCCGGY 1 cut(s) 730
Bse3DI GCAATG 2 cut(s) 803, 843
Bse8I GATNNNNATC 1 cut(s) 276
BseBI CCWGG 1 cut(s) 417
BseDI CCNNGG 2 cut(s) 416, 432
BseGI GGATG 2 cut(s) 201, 703
BseJI GATNNNNATC 1 cut(s) 276
BseLI CCNNNNNNNGG 4 cut(s) 364, 380, 602, 737
BseMI GCAATG 2 cut(s) 803, 843
BseMII CTCAG 2 cut(s) 722, 944
BsgI GTGCAG 1 cut(s) 384
BshFI GGCC 3 cut(s) 110, 397, 675
BsiHKAI GWGCWC 1 cut(s) 625
BsiSI CCGG 1 cut(s) 731
BslI CCNNNNNNNGG 4 cut(s) 364, 380, 602, 737
BsmAI GTCTC 4 cut(s) 46, 97, 422, 644
BsmBI CGTCTC 1 cut(s) 97
BsnI GGCC 3 cut(s) 110, 397, 675
Bso31I GGTCTC 2 cut(s) 422, 644
Bsp1286I GDGCHC 1 cut(s) 625
Bsp143I GATC 4 cut(s) 61, 502, 592, 982
Bsp19I CCATGG 1 cut(s) 432
BspACI CCGC 4 cut(s) 123, 365, 617, 728
BspANI GGCC 3 cut(s) 110, 397, 675
BspCNI CTCAG 2 cut(s) 721, 945
BspHI TCATGA 1 cut(s) 868
BspLI GGNNCC 1 cut(s) 31
BspPI GGATC 1 cut(s) 587
BspTNI GGTCTC 2 cut(s) 422, 644
BsrBI CCGCTC 1 cut(s) 617
BsrDI GCAATG 2 cut(s) 803, 843
BsrFI RCCGGY 1 cut(s) 730
BssAI RCCGGY 1 cut(s) 730
BssECI CCNNGG 2 cut(s) 416, 432
BssMI GATC 4 cut(s) 61, 502, 592, 982
BssT1I CCWWGG 1 cut(s) 432
Bst2UI CCWGG 1 cut(s) 417
Bst4CI ACNGT 4 cut(s) 23, 40, 210, 827
Bst6I CTCTTC 1 cut(s) 102
BstAPI GCANNNNNTGC 1 cut(s) 535
BstBAI YACGTR 3 cut(s) 136, 724, 897
BstC8I GCNNGC 3 cut(s) 399, 890, 959
BstDEI CTNAG 4 cut(s) 457, 670, 708, 953
BstDSI CCRYGG 1 cut(s) 432
BstF5I GGATG 2 cut(s) 201, 703
BstH2I RGCGCY 1 cut(s) 964
BstHHI GCGC 1 cut(s) 963
BstKTI GATC 4 cut(s) 64, 505, 595, 985
BstMAI GTCTC 4 cut(s) 46, 97, 422, 644
BstMBI GATC 4 cut(s) 61, 502, 592, 982
BstMWI GCNNNNNNNGC 1 cut(s) 535
BstNI CCWGG 1 cut(s) 417
BstNSI RCATGY 1 cut(s) 769
BstPAI GACNNNNGTC 1 cut(s) 636
BstSCI CCNGG 1 cut(s) 415
BstSFI CTRYAG 1 cut(s) 964
BstSNI TACGTA 1 cut(s) 724
BsuRI GGCC 3 cut(s) 110, 397, 675
BtgI CCRYGG 1 cut(s) 432
BtsCI GGATG 2 cut(s) 201, 703
Cac8I GCNNGC 3 cut(s) 399, 890, 959
CciI TCATGA 1 cut(s) 868
CfoI GCGC 1 cut(s) 963
Cfr10I RCCGGY 1 cut(s) 730
Cfr13I GGNCC 2 cut(s) 283, 383
Csp6I GTAC 1 cut(s) 725
CviAII CATG 7 cut(s) 146, 166, 287, 302, 433, 766, 869
CviQI GTAC 1 cut(s) 725
DdeI CTNAG 4 cut(s) 457, 670, 708, 953
DpnI GATC 4 cut(s) 63, 504, 594, 984
DpnII GATC 4 cut(s) 61, 502, 592, 982
Eam1104I CTCTTC 1 cut(s) 102
EarI CTCTTC 1 cut(s) 102
Ecl136II GAGCTC 1 cut(s) 623
Eco105I TACGTA 1 cut(s) 724
Eco130I CCWWGG 1 cut(s) 432
Eco147I AGGCCT 2 cut(s) 110, 675
Eco24I GRGCYC 1 cut(s) 625
Eco31I GGTCTC 2 cut(s) 422, 644
Eco32I GATATC 1 cut(s) 409
Eco47I GGWCC 2 cut(s) 283, 383
Eco47III AGCGCT 1 cut(s) 962
Eco53kI GAGCTC 1 cut(s) 623
Eco57I CTGAAG 1 cut(s) 369
EcoICRI GAGCTC 1 cut(s) 623
EcoRII CCWGG 1 cut(s) 415
EcoRV GATATC 1 cut(s) 409
EcoT14I CCWWGG 1 cut(s) 432
EcoT38I GRGCYC 1 cut(s) 625
ErhI CCWWGG 1 cut(s) 432
Esp3I CGTCTC 1 cut(s) 97
FaeI CATG 7 cut(s) 149, 169, 290, 305, 436, 769, 872
FalI AAGNNNNNCTT 2 cut(s) 522, 554
FatI CATG 7 cut(s) 145, 165, 286, 301, 432, 765, 868
FauI CCCGC 1 cut(s) 610
FokI GGATG 2 cut(s) 208, 710
FriOI GRGCYC 1 cut(s) 625
FspBI CTAG 4 cut(s) 480, 506, 770, 864
GlaI GCGC 1 cut(s) 962
GsuI CTGGAG 2 cut(s) 48, 799
HaeII RGCGCY 1 cut(s) 964
HaeIII GGCC 3 cut(s) 110, 397, 675
HapII CCGG 1 cut(s) 731
HhaI GCGC 1 cut(s) 963
Hin1II CATG 7 cut(s) 149, 169, 290, 305, 436, 769, 872
Hin6I GCGC 1 cut(s) 961
HinP1I GCGC 1 cut(s) 961
HindIII AAGCTT 1 cut(s) 227
HinfI GANTC 2 cut(s) 12, 754
HpaII CCGG 1 cut(s) 731
HphI GGTGA 2 cut(s) 74, 554
Hpy166II GTNNAC 2 cut(s) 206, 801
Hpy188I TCNGA 5 cut(s) 592, 614, 631, 907, 987
Hpy188III TCNNGA 6 cut(s) 269, 281, 500, 506, 830, 869
Hpy8I GTNNAC 2 cut(s) 206, 801
Hpy99I CGWCG 1 cut(s) 559
HpyAV CCTTC 1 cut(s) 572
HpyCH4III ACNGT 4 cut(s) 23, 40, 210, 827
HpyCH4IV ACGT 4 cut(s) 135, 557, 723, 896
HpyCH4V TGCA 6 cut(s) 401, 687, 789, 808, 848, 900
HpyF10VI GCNNNNNNNGC 1 cut(s) 535
HpyF3I CTNAG 4 cut(s) 457, 670, 708, 953
HpySE526I ACGT 4 cut(s) 135, 557, 723, 896
Hsp92II CATG 7 cut(s) 149, 169, 290, 305, 436, 769, 872
HspAI GCGC 1 cut(s) 961
Kzo9I GATC 4 cut(s) 61, 502, 592, 982
LmnI GCTCC 5 cut(s) 29, 614, 620, 740, 780
LweI GCATC 2 cut(s) 286, 688
MaeI CTAG 4 cut(s) 480, 506, 770, 864
MaeII ACGT 4 cut(s) 135, 557, 723, 896
MaeIII GTNAC 1 cut(s) 643
MalI GATC 4 cut(s) 63, 504, 594, 984
MbiI CCGCTC 1 cut(s) 617
MboI GATC 4 cut(s) 61, 502, 592, 982
MboII GAAGA 5 cut(s) 89, 121, 362, 449, 1010
MhlI GDGCHC 1 cut(s) 625
MluCI AATT 5 cut(s) 52, 161, 440, 606, 1002
MroXI GAANNNNTTC 1 cut(s) 441
MseI TTAA 2 cut(s) 735, 1005
MspI CCGG 1 cut(s) 731
MspR9I CCNGG 1 cut(s) 417
MvaI CCWGG 1 cut(s) 417
MwoI GCNNNNNNNGC 1 cut(s) 535
NcoI CCATGG 1 cut(s) 432
NdeII GATC 4 cut(s) 61, 502, 592, 982
NlaIII CATG 7 cut(s) 149, 169, 290, 305, 436, 769, 872
NlaIV GGNNCC 1 cut(s) 31
NmeAIII GCCGAG 1 cut(s) 23
NspI RCATGY 1 cut(s) 769
PagI TCATGA 1 cut(s) 868
PceI AGGCCT 2 cut(s) 110, 675
PdmI GAANNNNTTC 1 cut(s) 441
PfeI GAWTC 2 cut(s) 12, 754
PflMI CCANNNNNTGG 2 cut(s) 380, 602
Ppu21I YACGTR 3 cut(s) 136, 724, 897
PshAI GACNNNNGTC 1 cut(s) 636
Psp124BI GAGCTC 1 cut(s) 625
Psp6I CCWGG 1 cut(s) 415
PspGI CCWGG 1 cut(s) 415
PspN4I GGNNCC 1 cut(s) 31
PspPI GGNCC 2 cut(s) 283, 383
RsaI GTAC 1 cut(s) 726
RsaNI GTAC 1 cut(s) 725
SacI GAGCTC 1 cut(s) 625
SaqAI TTAA 2 cut(s) 735, 1005
Sau3AI GATC 4 cut(s) 61, 502, 592, 982
Sau96I GGNCC 2 cut(s) 283, 383
ScrFI CCNGG 1 cut(s) 417
SduI GDGCHC 1 cut(s) 625
SfaNI GCATC 2 cut(s) 286, 688
SfcI CTRYAG 1 cut(s) 964
SinI GGWCC 2 cut(s) 283, 383
SmlI CTYRAG 1 cut(s) 67
SmoI CTYRAG 1 cut(s) 67
SnaBI TACGTA 1 cut(s) 724
Sse9I AATT 5 cut(s) 52, 161, 440, 606, 1002
SseBI AGGCCT 2 cut(s) 110, 675
SsiI CCGC 4 cut(s) 123, 365, 617, 728
SspMI CTAG 4 cut(s) 480, 506, 770, 864
SstI GAGCTC 1 cut(s) 625
StuI AGGCCT 2 cut(s) 110, 675
StyD4I CCNGG 1 cut(s) 415
StyI CCWWGG 1 cut(s) 432
TaaI ACNGT 4 cut(s) 23, 40, 210, 827
TaiI ACGT 4 cut(s) 138, 560, 726, 899
TaqII GACCGA 1 cut(s) 626
TasI AATT 5 cut(s) 52, 161, 440, 606, 1002
TfiI GAWTC 2 cut(s) 12, 754
Tru1I TTAA 2 cut(s) 735, 1005
Tru9I TTAA 2 cut(s) 735, 1005
TspDTI ATGAA 7 cut(s) 154, 239, 290, 303, 434, 503, 591
TspGWI ACGGA 3 cut(s) 24, 79, 89
Van91I CCANNNNNTGG 2 cut(s) 380, 602
VpaK11BI GGWCC 2 cut(s) 283, 383
XapI RAATTY 3 cut(s) 161, 440, 606
XbaI TCTAGA 1 cut(s) 505
XceI RCATGY 1 cut(s) 769
XmnI GAANNNNTTC 1 cut(s) 441
XspI CTAG 4 cut(s) 480, 506, 770, 864
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.