Rorug02G0020400

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0008333
Physical Location & Seq
Reverse (-)
1645827 .. 1647573
1747 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0020400.1

Sequence Viewer

Length: 621 bp
ATGTCCAAAGCCCTAATGGAGGTCGAGGTCAAGCTGCGGCTTCCGGACGCCGCCGCCCACGGCCAGGTCACCACTCTTCTAGCGCCGTTCCACCTCGGCACCCACCGCCAGGAGAATCTCTTCTTCGACGGCTCCAAGTCCGAGCTCTCGGCGCGCCGGGCCGTGCTCCGGCTCCGCTTCTCCGACCGGAGCCCCCTCTGCGCCGTGACGCTGAAGGCGAAGGCGGTGCTGGTCGACGGCGTGAGCCGGGTGGAGGAGGACGAGGAGGAGATGGACCCGGCGGTGGGGCGGGCGTGCGCCGCCGAGCCGGAGAAGATGATGGCGGCGGAGTCTAGGGTTTTGAGGAGGGTGAGGGAGGAGTTTGGGGTTTTAGGGTTTGTGGGGATGGGAGGGTTTGGGAATGTGAGGGATGTGTATGATTGGAAAGGGTTGAAATTGGAGGTGGATGAGACTAAGTATGAGTTTGGGACTTGTTATGAGATTGAGTGTGAGAGCGCTGATCCTGATGGGGCTAAGGAGAAGCTTGAGGAGTTCTTGAAGGAAAATGGGGTGAGGTATTCGTATTCTCAAGTGTCCAAGTTTGCGACTTTTCGAGCTGGGAAGCTGCCTTCAATGGGGTGA

Protein Analysis

206

Amino Acids

22.94

Weight (kDa)

5.44

Isoelectric Point (pI)

47.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CYTH PF01928 6 - 183 1.8e-19 CYTH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 98
AccI GTMKAC 1 cut(s) 234
AccII CGCG 1 cut(s) 154
AccIII TCCGGA 1 cut(s) 43
AclWI GGATC 1 cut(s) 494
AcoI YGGCCR 1 cut(s) 61
AcuI CTGAAG 1 cut(s) 233
AcyI GRCGYC 1 cut(s) 48
AfeI AGCGCT 1 cut(s) 496
AfiI CCNNNNNNNGG 8 cut(s) 19, 64, 109, 168, 253, 283, 284, 614
AgsI TTSAA 3 cut(s) 433, 538, 612
AjnI CCWGG 2 cut(s) 63, 108
AluBI AGCT 5 cut(s) 34, 145, 523, 596, 604
AluI AGCT 5 cut(s) 34, 145, 523, 596, 604
Alw21I GWGCWC 2 cut(s) 147, 168
Alw26I GTCTC 1 cut(s) 443
AlwI GGATC 1 cut(s) 494
Aor13HI TCCGGA 1 cut(s) 43
Aor51HI AGCGCT 1 cut(s) 496
AoxI GGCC 2 cut(s) 61, 159
ApeKI GCWGC 2 cut(s) 34, 604
AscI GGCGCGCC 1 cut(s) 152
Asp700I GAANNNNTTC 1 cut(s) 119
AspLEI GCGC 6 cut(s) 85, 154, 156, 203, 299, 497
AspS9I GGNCC 2 cut(s) 159, 274
AsuC2I CCSGG 3 cut(s) 158, 248, 278
AsuHPI GGTGA 3 cut(s) 61, 361, 562
AvaII GGWCC 1 cut(s) 274
BanI GGYRCC 1 cut(s) 98
BanII GRGCYC 2 cut(s) 147, 194
Bbv12I GWGCWC 2 cut(s) 147, 168
BbvI GCAGC 2 cut(s) 21, 591
BccI CCATC 4 cut(s) 265, 313, 379, 500
BceAI ACGGC 6 cut(s) 70, 76, 145, 146, 188, 253
BcgI CGANNNNNNTGC 2 cut(s) 208, 242
BciT130I CCWGG 2 cut(s) 65, 110
BcnI CCSGG 3 cut(s) 158, 248, 278
BcoDI GTCTC 1 cut(s) 443
BfaI CTAG 2 cut(s) 80, 333
BfoI RGCGCY 2 cut(s) 86, 498
BisI GCNGC 7 cut(s) 35, 38, 51, 54, 300, 324, 605
BlsI GCNGC 7 cut(s) 36, 39, 52, 55, 301, 325, 606
Bme1390I CCNGG 5 cut(s) 65, 110, 158, 248, 278
Bme18I GGWCC 1 cut(s) 274
BmgT120I GGNCC 2 cut(s) 159, 274
BmiI GGNNCC 5 cut(s) 100, 133, 173, 191, 276
BmrFI CCNGG 5 cut(s) 65, 110, 158, 248, 278
Bpu10I CCTNAGC 1 cut(s) 513
BpuEI CTTGAG 2 cut(s) 545, 552
BpuMI CCSGG 3 cut(s) 158, 248, 278
BsaHI GRCGYC 1 cut(s) 48
BsaJI CCNNGG 2 cut(s) 58, 94
BsaWI WCCGGW 2 cut(s) 43, 186
Bsc4I CCNNNNNNNGG 8 cut(s) 19, 64, 109, 168, 253, 283, 284, 614
BseAI TCCGGA 1 cut(s) 43
BseBI CCWGG 2 cut(s) 65, 110
BseDI CCNNGG 2 cut(s) 58, 94
BseGI GGATG 3 cut(s) 390, 415, 451
BseLI CCNNNNNNNGG 8 cut(s) 19, 64, 109, 168, 253, 283, 284, 614
BsePI GCGCGC 1 cut(s) 152
BseRI GAGGAG 6 cut(s) 269, 278, 281, 358, 371, 542
BseXI GCAGC 2 cut(s) 21, 591
BseYI CCCAGC 1 cut(s) 596
Bsh1236I CGCG 1 cut(s) 154
Bsh1285I CGRYCG 1 cut(s) 187
BshFI GGCC 2 cut(s) 63, 161
BshNI GGYRCC 1 cut(s) 98
BsiEI CGRYCG 1 cut(s) 187
BsiHKAI GWGCWC 2 cut(s) 147, 168
BsiSI CCGG 7 cut(s) 44, 157, 169, 187, 247, 278, 308
BslFI GGGAC 1 cut(s) 481
BslI CCNNNNNNNGG 8 cut(s) 19, 64, 109, 168, 253, 283, 284, 614
BsmAI GTCTC 1 cut(s) 443
BsmFI GGGAC 1 cut(s) 481
BsnI GGCC 2 cut(s) 63, 161
Bsp1286I GDGCHC 3 cut(s) 147, 168, 194
Bsp13I TCCGGA 1 cut(s) 43
Bsp143I GATC 1 cut(s) 499
BspANI GGCC 2 cut(s) 63, 161
BspEI TCCGGA 1 cut(s) 43
BspFNI CGCG 1 cut(s) 154
BspLI GGNNCC 5 cut(s) 100, 133, 173, 191, 276
BspPI GGATC 1 cut(s) 494
BspT107I GGYRCC 1 cut(s) 98
BssECI CCNNGG 2 cut(s) 58, 94
BssHII GCGCGC 1 cut(s) 152
BssMI GATC 1 cut(s) 499
BssNI GRCGYC 1 cut(s) 48
Bst2UI CCWGG 2 cut(s) 65, 110
Bst6I CTCTTC 2 cut(s) 81, 125
BstACI GRCGYC 1 cut(s) 48
BstC8I GCNNGC 3 cut(s) 154, 291, 295
BstDEI CTNAG 2 cut(s) 453, 513
BstDSI CCRYGG 1 cut(s) 58
BstEII GGTNACC 1 cut(s) 67
BstENI CCTNNNNNAGG 1 cut(s) 17
BstF5I GGATG 3 cut(s) 390, 415, 451
BstFNI CGCG 1 cut(s) 154
BstH2I RGCGCY 2 cut(s) 86, 498
BstHHI GCGC 6 cut(s) 85, 154, 156, 203, 299, 497
BstKTI GATC 1 cut(s) 502
BstMAI GTCTC 1 cut(s) 443
BstMBI GATC 1 cut(s) 499
BstMCI CGRYCG 1 cut(s) 187
BstMWI GCNNNNNNNGC 5 cut(s) 105, 151, 158, 198, 299
BstNI CCWGG 2 cut(s) 65, 110
BstPI GGTNACC 1 cut(s) 67
BstSCI CCNGG 5 cut(s) 63, 108, 156, 246, 276
BstUI CGCG 1 cut(s) 154
BstV1I GCAGC 2 cut(s) 21, 591
BsuRI GGCC 2 cut(s) 63, 161
BtgI CCRYGG 1 cut(s) 58
BtsCI GGATG 3 cut(s) 390, 415, 451
Cac8I GCNNGC 3 cut(s) 154, 291, 295
CfoI GCGC 6 cut(s) 85, 154, 156, 203, 299, 497
Cfr13I GGNCC 2 cut(s) 159, 274
CseI GACGC 2 cut(s) 56, 217
DdeI CTNAG 2 cut(s) 453, 513
DpnI GATC 1 cut(s) 501
DpnII GATC 1 cut(s) 499
EaeI YGGCCR 1 cut(s) 61
Eam1104I CTCTTC 2 cut(s) 81, 125
EarI CTCTTC 2 cut(s) 81, 125
EciI GGCGGA 1 cut(s) 341
Ecl136II GAGCTC 1 cut(s) 145
Eco24I GRGCYC 2 cut(s) 147, 194
Eco47I GGWCC 1 cut(s) 274
Eco47III AGCGCT 1 cut(s) 496
Eco53kI GAGCTC 1 cut(s) 145
Eco57I CTGAAG 1 cut(s) 233
Eco91I GGTNACC 1 cut(s) 67
EcoICRI GAGCTC 1 cut(s) 145
EcoNI CCTNNNNNAGG 1 cut(s) 17
EcoO65I GGTNACC 1 cut(s) 67
EcoRII CCWGG 2 cut(s) 63, 108
EcoT38I GRGCYC 2 cut(s) 147, 194
FaiI YATR 3 cut(s) 417, 459, 477
FaqI GGGAC 1 cut(s) 481
FauI CCCGC 1 cut(s) 282
FblI GTMKAC 1 cut(s) 234
Fnu4HI GCNGC 7 cut(s) 35, 38, 51, 54, 300, 324, 605
FokI GGATG 3 cut(s) 397, 422, 458
FriOI GRGCYC 2 cut(s) 147, 194
Fsp4HI GCNGC 7 cut(s) 35, 38, 51, 54, 300, 324, 605
FspBI CTAG 2 cut(s) 80, 333
GlaI GCGC 6 cut(s) 84, 153, 155, 202, 298, 496
GluI GCNGC 7 cut(s) 35, 38, 51, 54, 300, 324, 605
GsaI CCCAGC 1 cut(s) 600
HaeII RGCGCY 2 cut(s) 86, 498
HaeIII GGCC 2 cut(s) 63, 161
HapII CCGG 7 cut(s) 44, 157, 169, 187, 247, 278, 308
HgaI GACGC 2 cut(s) 56, 217
HhaI GCGC 6 cut(s) 85, 154, 156, 203, 299, 497
Hin1I GRCGYC 1 cut(s) 48
Hin6I GCGC 6 cut(s) 83, 152, 154, 201, 297, 495
HinP1I GCGC 6 cut(s) 83, 152, 154, 201, 297, 495
HincII GTYRAC 1 cut(s) 235
HindII GTYRAC 1 cut(s) 235
HindIII AAGCTT 1 cut(s) 521
HinfI GANTC 2 cut(s) 115, 329
HpaII CCGG 7 cut(s) 44, 157, 169, 187, 247, 278, 308
HphI GGTGA 3 cut(s) 61, 361, 562
Hpy166II GTNNAC 1 cut(s) 235
Hpy188I TCNGA 2 cut(s) 142, 184
Hpy188III TCNNGA 3 cut(s) 44, 503, 535
Hpy8I GTNNAC 1 cut(s) 235
Hpy99I CGWCG 2 cut(s) 131, 239
HpyAV CCTTC 4 cut(s) 208, 214, 532, 618
HpyF10VI GCNNNNNNNGC 5 cut(s) 105, 151, 158, 198, 299
HpyF3I CTNAG 2 cut(s) 453, 513
Hsp92I GRCGYC 1 cut(s) 48
HspAI GCGC 6 cut(s) 83, 152, 154, 201, 297, 495
Kpn2I TCCGGA 1 cut(s) 43
Kzo9I GATC 1 cut(s) 499
LmnI GCTCC 4 cut(s) 137, 171, 177, 189
Lsp1109I GCAGC 2 cut(s) 21, 591
MaeI CTAG 2 cut(s) 80, 333
MaeIII GTNAC 2 cut(s) 67, 205
MalI GATC 1 cut(s) 501
MboI GATC 1 cut(s) 499
MboII GAAGA 4 cut(s) 68, 112, 115, 325
MhlI GDGCHC 3 cut(s) 147, 168, 194
MluCI AATT 1 cut(s) 434
MlyI GAGTC 1 cut(s) 338
MmeI TCCRAC 1 cut(s) 207
MroI TCCGGA 1 cut(s) 43
MroXI GAANNNNTTC 1 cut(s) 119
MspI CCGG 7 cut(s) 44, 157, 169, 187, 247, 278, 308
MspR9I CCNGG 5 cut(s) 65, 110, 158, 248, 278
MvaI CCWGG 2 cut(s) 65, 110
MvnI CGCG 1 cut(s) 154
MwoI GCNNNNNNNGC 5 cut(s) 105, 151, 158, 198, 299
NciI CCSGG 3 cut(s) 158, 248, 278
NdeII GATC 1 cut(s) 499
NlaIV GGNNCC 5 cut(s) 100, 133, 173, 191, 276
NmeAIII GCCGAG 3 cut(s) 75, 128, 328
NmuCI GTSAC 2 cut(s) 67, 205
PalAI GGCGCGCC 1 cut(s) 152
PauI GCGCGC 1 cut(s) 152
PcsI WCGNNNNNNNCGW 1 cut(s) 215
PdmI GAANNNNTTC 1 cut(s) 119
PfeI GAWTC 1 cut(s) 115
PkrI GCNGC 7 cut(s) 36, 39, 52, 55, 301, 325, 606
PleI GAGTC 1 cut(s) 337
PpsI GAGTC 1 cut(s) 337
Psp124BI GAGCTC 1 cut(s) 147
Psp6I CCWGG 2 cut(s) 63, 108
PspEI GGTNACC 1 cut(s) 67
PspFI CCCAGC 1 cut(s) 596
PspGI CCWGG 2 cut(s) 63, 108
PspN4I GGNNCC 5 cut(s) 100, 133, 173, 191, 276
PspPI GGNCC 2 cut(s) 159, 274
PteI GCGCGC 1 cut(s) 152
SacI GAGCTC 1 cut(s) 147
SalI GTCGAC 1 cut(s) 233
SatI GCNGC 7 cut(s) 35, 38, 51, 54, 300, 324, 605
Sau3AI GATC 1 cut(s) 499
Sau96I GGNCC 2 cut(s) 159, 274
SchI GAGTC 1 cut(s) 338
ScrFI CCNGG 5 cut(s) 65, 110, 158, 248, 278
SduI GDGCHC 3 cut(s) 147, 168, 194
SgsI GGCGCGCC 1 cut(s) 152
SinI GGWCC 1 cut(s) 274
SmlI CTYRAG 2 cut(s) 524, 567
SmoI CTYRAG 2 cut(s) 524, 567
Sse9I AATT 1 cut(s) 434
SspMI CTAG 2 cut(s) 80, 333
SstI GAGCTC 1 cut(s) 147
StyD4I CCNGG 5 cut(s) 63, 108, 156, 246, 276
TaqI TCGA 4 cut(s) 24, 126, 234, 592
TasI AATT 1 cut(s) 434
TauI GCSGC 5 cut(s) 40, 53, 56, 302, 326
TfiI GAWTC 1 cut(s) 115
TseFI GTSAC 2 cut(s) 67, 205
TseI GCWGC 2 cut(s) 34, 604
Tsp45I GTSAC 2 cut(s) 67, 205
VpaK11BI GGWCC 1 cut(s) 274
XagI CCTNNNNNAGG 1 cut(s) 17
XcmI CCANNNNNNNNNTGG 1 cut(s) 13
XmiI GTMKAC 1 cut(s) 234
XmnI GAANNNNTTC 1 cut(s) 119
XspI CTAG 2 cut(s) 80, 333
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.