Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0008333

Physical Location & Seq

Genomic Coordinates Reverse (-)

5258537 .. 5261632

Gene Structure 3096 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1064900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1011 bp

ATGGCAACCCACATCACAAACGGCGACGGAGTTGCCAGAGTCTCCGCCCTCAACTGCATCGATCTCTCGAGCTCCGACACCCACCAATCCGTCTCTTTACTCAAACAGGCGTGTTTGGATTGCGGGTTTTTCTACCTGGTGAATCATGGAATAAGCGAGGAATTCATGGCGGAGGTGTTTGATCAGAGCAGGAGGCTTTTCGAGTTGCCATTGAGTGCAAAAATGGAGCTTTTGAGGAACAAGAATTACAGGGGATATACCCCTTCTCTCGACGAGCATCTCGATCATGAAAACCAAGTTCACGGAGATTACAAGGAGGGGTATTACATAGGAGTTGAGCTACAAGAAGATGACCCAGAAGCTAAAAAGCCGTTTTATGGGCCTAATGTTTGGCCTGCTCCAGATGTGTTGCCGGGGTGGAGGGAGACCATGGAGGAATATCATAGACAATGCTTGGAGGTGGCTAAGTCAGTTGGTAGGCTTGTGGCCCTTGCTCTTGATCTCGACATCCATTTCTTCGACAAACCAGAAATGCTTGGCGAGGCTATAGCAACACTGCGTTTACTACACTACGATGGTCAAGTTTCTGATCCAGCAAATGGAATTTTTGGAGCTGGAGCTCATTCGGACTTTGGTTTTATAACCCTATTGGCAACAGATGATGTTGCAGGTCTCCAAATATGCAAGGATAAGGATGCGAAACCTCAAATATGGGAATATATACCACCAATAAAAGGGGTATTTATAGTGAATATTGGTGACATGCTGGAACGCTGGAGCAACGGCATTTTCAAGTCCACATTGCATAGAGTTGTAGGGAACGGTCAAGACAGATACTCTATCGCATACTTTATAGAACCAAGTCACGATTGTCTTGTTGAGTGTTTGCCTACGTGCAAATCTGAAAAGAACCCTCCCAAATTTCCCCCGATCTTGTGCCGCACTTACCTGAGCCAGCGCTATTACAATACTCACGCCAATCTGAATGTCTACACCGAACATAACAAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0007275 GO:0008150 GO:0009653 GO:0009791 GO:0010015 GO:0010101 GO:0010102 GO:0010468 GO:0019222 GO:0022622 GO:0032501 GO:0032502 GO:0048364 GO:0048527 GO:0048528 GO:0048731 GO:0048856 GO:0050789 GO:0060255 GO:0065007 GO:0090696 GO:0090697 GO:0090698 GO:0099402 GO:1905392

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

2OG-FeII_Oxy DIOX_N

Protein Analysis

337

Amino Acids

38.03

Weight (kDa)

5.28

Isoelectric Point (pI)

43.81

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DIOX_N	PF14226	18 - 133	1.2e-34	non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy	PF03171	189 - 287	6.7e-25	2OG-Fe(II) oxygenase superfamily

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008333)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G35190 AT1G35190
fragaria_vesca	FvH4_1g06010 FvH4_1g06010 FvH4_1g06010 FvH4_1g06010 FvH4_1g06010
malus_domestica	MD02G1064900.v1.1
prunus_persica	Prupe.7G221000_v2.0.a1 Prupe.7G221000_v2.0.a1
pyrus_communis	pycom02g05100 pycom03g18760 pycom15g17490
rosa_chinensis	RchiOBHm_Chr2g0091751
rosa_laevigata	RLG00000016249
rosa_multiflora	Rmu_sc0000897.1_g000004
rosa_roxburghii	Rroxscaffold_2G00149770
rosa_rugosa	Rorug02G0020300 Rorug02G0020400 Rorug02G0020500
rosa_samantha	Rh2CG065900 Rh2DG064400
rosa_wichuraiana	Rw2G005540

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	641
Acc36I	ACCTGC	1 cut(s)	659
AccB7I	CCANNNNNTGG	1 cut(s)	599
AccI	GTMKAC	1 cut(s)	990
AciI	CCGC	4 cut(s)	45, 123, 170, 940
AclWI	GGATC	1 cut(s)	584
AcsI	RAATTY	3 cut(s)	161, 603, 920
AfeI	AGCGCT	1 cut(s)	959
AfiI	CCNNNNNNNGG	3 cut(s)	377, 599, 734
AgsI	TTSAA	1 cut(s)	793
AjnI	CCWGG	1 cut(s)	135
AluBI	AGCT	6 cut(s)	72, 229, 340, 362, 614, 620
AluI	AGCT	6 cut(s)	72, 229, 340, 362, 614, 620
Alw21I	GWGCWC	2 cut(s)	74, 622
Alw26I	GTCTC	4 cut(s)	46, 97, 419, 677
AlwI	GGATC	1 cut(s)	584
Ama87I	CYCGRG	1 cut(s)	67
Aor51HI	AGCGCT	1 cut(s)	959
AoxI	GGCC	3 cut(s)	380, 392, 486
ApoI	RAATTY	3 cut(s)	161, 603, 920
AspLEI	GCGC	1 cut(s)	960
AspS9I	GGNCC	2 cut(s)	380, 487
AsuC2I	CCSGG	1 cut(s)	414
AsuHPI	GGTGA	2 cut(s)	151, 770
AvaI	CYCGRG	1 cut(s)	67
BanII	GRGCYC	2 cut(s)	74, 622
Bbv12I	GWGCWC	2 cut(s)	74, 622
BccI	CCATC	1 cut(s)	569
BceAI	ACGGC	3 cut(s)	37, 355, 799
BcgI	CGANNNNNNTGC	2 cut(s)	14, 48
BciT130I	CCWGG	1 cut(s)	137
BclI	TGATCA	1 cut(s)	181
BcnI	CCSGG	1 cut(s)	414
BcoDI	GTCTC	4 cut(s)	46, 97, 419, 677
BfmI	CTRYAG	1 cut(s)	546
BfoI	RGCGCY	1 cut(s)	961
BfuAI	ACCTGC	1 cut(s)	659
BisI	GCNGC	1 cut(s)	940
BlsI	GCNGC	1 cut(s)	941
Bme1390I	CCNGG	2 cut(s)	137, 414
BmeT110I	CYCGRG	1 cut(s)	67
BmgT120I	GGNCC	2 cut(s)	380, 487
BmrFI	CCNGG	2 cut(s)	137, 414
BmsI	GCATC	3 cut(s)	66, 286, 685
BpmI	CTGGAG	3 cut(s)	384, 636, 796
Bpu10I	CCTNAGC	1 cut(s)	950
BpuMI	CCSGG	1 cut(s)	414
Bsa29I	ATCGAT	1 cut(s)	60
BsaAI	YACGTR	1 cut(s)	894
BsaI	GGTCTC	2 cut(s)	419, 677
BsaJI	CCNNGG	2 cut(s)	413, 429
Bsc4I	CCNNNNNNNGG	3 cut(s)	377, 599, 734
Bse3DI	GCAATG	1 cut(s)	800
BseBI	CCWGG	1 cut(s)	137
BseCI	ATCGAT	1 cut(s)	60
BseDI	CCNNGG	2 cut(s)	413, 429
BseGI	GGATG	2 cut(s)	507, 700
BseLI	CCNNNNNNNGG	3 cut(s)	377, 599, 734
BseMI	GCAATG	1 cut(s)	800
BseMII	CTCAG	1 cut(s)	941
BshFI	GGCC	3 cut(s)	382, 394, 488
BshVI	ATCGAT	1 cut(s)	60
BsiHKAI	GWGCWC	2 cut(s)	74, 622
BsiHKCI	CYCGRG	1 cut(s)	67
BsiSI	CCGG	1 cut(s)	413
BslI	CCNNNNNNNGG	3 cut(s)	377, 599, 734
BsmAI	GTCTC	4 cut(s)	46, 97, 419, 677
BsmBI	CGTCTC	1 cut(s)	97
BsnI	GGCC	3 cut(s)	382, 394, 488
Bso31I	GGTCTC	2 cut(s)	419, 677
BsoBI	CYCGRG	1 cut(s)	67
Bsp1286I	GDGCHC	2 cut(s)	74, 622
Bsp143I	GATC	6 cut(s)	61, 181, 283, 499, 589, 930
Bsp19I	CCATGG	1 cut(s)	429
BspACI	CCGC	4 cut(s)	45, 123, 170, 940
BspANI	GGCC	3 cut(s)	382, 394, 488
BspCNI	CTCAG	1 cut(s)	942
BspDI	ATCGAT	1 cut(s)	60
BspHI	TCATGA	1 cut(s)	286
BspMI	ACCTGC	1 cut(s)	659
BspPI	GGATC	1 cut(s)	584
BspTNI	GGTCTC	2 cut(s)	419, 677
BsrDI	GCAATG	1 cut(s)	800
BssECI	CCNNGG	2 cut(s)	413, 429
BssMI	GATC	6 cut(s)	61, 181, 283, 499, 589, 930
BssT1I	CCWWGG	1 cut(s)	429
Bst2UI	CCWGG	1 cut(s)	137
Bst4CI	ACNGT	1 cut(s)	824
BstBAI	YACGTR	1 cut(s)	894
BstC8I	GCNNGC	2 cut(s)	396, 956
BstDEI	CTNAG	2 cut(s)	465, 950
BstDSI	CCRYGG	1 cut(s)	429
BstF5I	GGATG	2 cut(s)	507, 700
BstH2I	RGCGCY	1 cut(s)	961
BstHHI	GCGC	1 cut(s)	960
BstKTI	GATC	6 cut(s)	64, 184, 286, 502, 592, 933
BstMAI	GTCTC	4 cut(s)	46, 97, 419, 677
BstMBI	GATC	6 cut(s)	61, 181, 283, 499, 589, 930
BstNI	CCWGG	1 cut(s)	137
BstNSI	RCATGY	1 cut(s)	766
BstSCI	CCNGG	2 cut(s)	135, 412
BstSFI	CTRYAG	1 cut(s)	546
Bsu15I	ATCGAT	1 cut(s)	60
BsuRI	GGCC	3 cut(s)	382, 394, 488
BsuTUI	ATCGAT	1 cut(s)	60
BtgI	CCRYGG	1 cut(s)	429
BtsCI	GGATG	2 cut(s)	507, 700
BtsI	GCAGTG	1 cut(s)	554
BtsIMutI	CAGTG	1 cut(s)	554
BveI	ACCTGC	1 cut(s)	659
Cac8I	GCNNGC	2 cut(s)	396, 956
CciI	TCATGA	1 cut(s)	286
CfoI	GCGC	1 cut(s)	960
Cfr13I	GGNCC	2 cut(s)	380, 487
ClaI	ATCGAT	1 cut(s)	60
CsiI	ACCWGGT	1 cut(s)	135
CviAII	CATG	5 cut(s)	146, 166, 287, 430, 763
DdeI	CTNAG	2 cut(s)	465, 950
DpnI	GATC	6 cut(s)	63, 183, 285, 501, 591, 932
DpnII	GATC	6 cut(s)	61, 181, 283, 499, 589, 930
EciI	GGCGGA	2 cut(s)	34, 185
Ecl136II	GAGCTC	2 cut(s)	72, 620
Eco130I	CCWWGG	1 cut(s)	429
Eco24I	GRGCYC	2 cut(s)	74, 622
Eco31I	GGTCTC	2 cut(s)	419, 677
Eco47III	AGCGCT	1 cut(s)	959
Eco53kI	GAGCTC	2 cut(s)	72, 620
Eco88I	CYCGRG	1 cut(s)	67
EcoICRI	GAGCTC	2 cut(s)	72, 620
EcoRI	GAATTC	1 cut(s)	161
EcoRII	CCWGG	1 cut(s)	135
EcoT14I	CCWWGG	1 cut(s)	429
EcoT38I	GRGCYC	2 cut(s)	74, 622
ErhI	CCWWGG	1 cut(s)	429
Esp3I	CGTCTC	1 cut(s)	97
FaeI	CATG	5 cut(s)	149, 169, 290, 433, 766
FatI	CATG	5 cut(s)	145, 165, 286, 429, 762
FauI	CCCGC	1 cut(s)	116
FbaI	TGATCA	1 cut(s)	181
FblI	GTMKAC	1 cut(s)	990
Fnu4HI	GCNGC	1 cut(s)	940
FokI	GGATG	2 cut(s)	494, 707
FriOI	GRGCYC	2 cut(s)	74, 622
Fsp4HI	GCNGC	1 cut(s)	940
GlaI	GCGC	1 cut(s)	959
GluI	GCNGC	1 cut(s)	940
GsuI	CTGGAG	3 cut(s)	384, 636, 796
HaeII	RGCGCY	1 cut(s)	961
HaeIII	GGCC	3 cut(s)	382, 394, 488
HapII	CCGG	1 cut(s)	413
HhaI	GCGC	1 cut(s)	960
Hin1II	CATG	5 cut(s)	149, 169, 290, 433, 766
Hin6I	GCGC	1 cut(s)	958
HinP1I	GCGC	1 cut(s)	958
HinfI	GANTC	2 cut(s)	39, 142
HpaII	CCGG	1 cut(s)	413
HphI	GGTGA	2 cut(s)	151, 770
Hpy166II	GTNNAC	4 cut(s)	301, 563, 798, 991
Hpy188I	TCNGA	6 cut(s)	76, 186, 589, 628, 904, 984
Hpy188III	TCNNGA	9 cut(s)	67, 269, 281, 287, 401, 497, 503, 827, 866
Hpy8I	GTNNAC	4 cut(s)	301, 563, 798, 991
Hpy99I	CGWCG	2 cut(s)	29, 275
HpyAV	CCTTC	1 cut(s)	273
HpyCH4III	ACNGT	1 cut(s)	824
HpyCH4IV	ACGT	1 cut(s)	893
HpyCH4V	TGCA	6 cut(s)	57, 218, 668, 684, 805, 897
HpyF3I	CTNAG	2 cut(s)	465, 950
HpySE526I	ACGT	1 cut(s)	893
Hsp92II	CATG	5 cut(s)	149, 169, 290, 433, 766
HspAI	GCGC	1 cut(s)	958
Ksp22I	TGATCA	1 cut(s)	181
Kzo9I	GATC	6 cut(s)	61, 181, 283, 499, 589, 930
LmnI	GCTCC	6 cut(s)	77, 226, 403, 611, 617, 777
LweI	GCATC	3 cut(s)	66, 286, 685
MabI	ACCWGGT	1 cut(s)	135
MaeII	ACGT	1 cut(s)	893
MaeIII	GTNAC	2 cut(s)	758, 863
MalI	GATC	6 cut(s)	63, 183, 285, 501, 591, 932
MboI	GATC	6 cut(s)	61, 181, 283, 499, 589, 930
MboII	GAAGA	2 cut(s)	359, 508
MhlI	GDGCHC	2 cut(s)	74, 622
MluCI	AATT	4 cut(s)	161, 244, 603, 920
MlyI	GAGTC	1 cut(s)	48
MmeI	TCCRAC	1 cut(s)	99
MslI	CAYNNNNRTG	1 cut(s)	573
MspI	CCGG	1 cut(s)	413
MspR9I	CCNGG	2 cut(s)	137, 414
MvaI	CCWGG	1 cut(s)	137
NciI	CCSGG	1 cut(s)	414
NcoI	CCATGG	1 cut(s)	429
NdeII	GATC	6 cut(s)	61, 181, 283, 499, 589, 930
NlaIII	CATG	5 cut(s)	149, 169, 290, 433, 766
NmuCI	GTSAC	2 cut(s)	758, 863
NspI	RCATGY	1 cut(s)	766
PaeR7I	CTCGAG	1 cut(s)	67
PagI	TCATGA	1 cut(s)	286
PcsI	WCGNNNNNNNCGW	1 cut(s)	279
PfeI	GAWTC	1 cut(s)	142
PflMI	CCANNNNNTGG	1 cut(s)	599
PkrI	GCNGC	1 cut(s)	941
PleI	GAGTC	1 cut(s)	47
PpsI	GAGTC	1 cut(s)	47
Ppu21I	YACGTR	1 cut(s)	894
PsiI	TTATAA	1 cut(s)	641
Psp124BI	GAGCTC	2 cut(s)	74, 622
Psp6I	CCWGG	1 cut(s)	135
PspGI	CCWGG	1 cut(s)	135
PspPI	GGNCC	2 cut(s)	380, 487
PsrI	GAACNNNNNNTAC	2 cut(s)	230, 262
RseI	CAYNNNNRTG	1 cut(s)	573
SacI	GAGCTC	2 cut(s)	74, 622
SatI	GCNGC	1 cut(s)	940
Sau3AI	GATC	6 cut(s)	61, 181, 283, 499, 589, 930
Sau96I	GGNCC	2 cut(s)	380, 487
SchI	GAGTC	1 cut(s)	48
ScrFI	CCNGG	2 cut(s)	137, 414
SduI	GDGCHC	2 cut(s)	74, 622
SexAI	ACCWGGT	1 cut(s)	135
SfaNI	GCATC	3 cut(s)	66, 286, 685
SfcI	CTRYAG	1 cut(s)	546
Sfr274I	CTCGAG	1 cut(s)	67
SlaI	CTCGAG	1 cut(s)	67
SmiMI	CAYNNNNRTG	1 cut(s)	573
SmlI	CTYRAG	1 cut(s)	67
SmoI	CTYRAG	1 cut(s)	67
Sse9I	AATT	4 cut(s)	161, 244, 603, 920
SsiI	CCGC	4 cut(s)	45, 123, 170, 940
SspI	AATATT	1 cut(s)	754
SstI	GAGCTC	2 cut(s)	74, 622
StyD4I	CCNGG	2 cut(s)	135, 412
StyI	CCWWGG	1 cut(s)	429
TaaI	ACNGT	1 cut(s)	824
TaiI	ACGT	1 cut(s)	896
TaqI	TCGA	7 cut(s)	60, 68, 201, 270, 282, 504, 519
TasI	AATT	4 cut(s)	161, 244, 603, 920
TauI	GCSGC	1 cut(s)	942
TfiI	GAWTC	1 cut(s)	142
TscAI	CASTG	1 cut(s)	561
TseFI	GTSAC	2 cut(s)	758, 863
Tsp45I	GTSAC	2 cut(s)	758, 863
TspDTI	ATGAA	2 cut(s)	154, 303
TspGWI	ACGGA	3 cut(s)	42, 79, 318
TspRI	CASTG	1 cut(s)	561
Van91I	CCANNNNNTGG	1 cut(s)	599
XapI	RAATTY	3 cut(s)	161, 603, 920
XceI	RCATGY	1 cut(s)	766
XhoI	CTCGAG	1 cut(s)	67
XmiI	GTMKAC	1 cut(s)	990

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.