FvH4_1g09760

Diacylglycerol O-acyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0006124
Physical Location & Seq
Reverse (-)
5309138 .. 5313368
4231 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g09760.t2

Sequence Viewer

Length: 981 bp
ATGGTGGAGATGAACAAGGAAGAAGAGAAGGAGGGAGTGACGATAATCAAAGGAAAAGAGGCTATTGGATTGAGAATCGTCAACATGTTTGTGGCGATGGCTTTGTGGCTTGGAGCTATTCATTTCAACGTGGCTTTGATTGTCCTCACCGTTTGCTTCCTTCCTATTTCCAAGGCCATATTGGTATTTGGGTTGCTTGTGCTGTTTATGATAGTCCCAGTTGATGATAAGAGCAAAATAGGAAGAAGAGTGGCCAGGTACATATGTAAGTATGCATGTGGTTATTTCCCAGTGACTTTGTATGTAGAGGAAAAACATGTGTTTGATCCCAATTGCGCTTATGTCTTTGGTTACGAACCACATTCAGTTTTGCCAATTGGTGTTGTTGCACTTGCTGACATGACTGGGTTCTTGGGTCTCCCTAAGATGAAGGTCCTTGCAAGTACTGCTGTGTTCTACACACCATTCCTAAGACATATATGGACATGGTTGGGTCTTACACCAGCCACAAAGAGCAATTTTGTCTCCAACTTGGCTGCTGGTTATAGTTGCGTCATTGTACCTGGTGGAGTGCAGGAGACATTTCTTATGGAGCACGGTAAAGAGAAAGTATTTCTTAAGTCGAGAAGAGGATTTGTTCGTATAGCCCTGGAGAATGGCTATCCCCTGGTTCCAGTTTTCTGCTTTGGTCAGTCAAATGTTTATAAGTGGTGGAAGCCAAGTGGGGAGTTATTCCTGAAGTTTGCTAGAGCTATCAAGTTCACCCCTATTGTCTTCTGGGGAATGTTTGGGTCCCCTTTACCTTTCAGACATCCAATGCATGTGGTAGTTGGTAAACCAATAGAACTCAAGAAAAATCCAGAACCCACCATGGAAGAGGTCATGGAAGTACATGGTCAGTATGTTGAAGCACTGAAGGAGCTTTTTGAAAAGCACAAGGCACGGGCTGGTTTTCCTGACCTTGAATTGGAAATACTTTGA

Protein Analysis

327

Amino Acids

36.8

Weight (kDa)

8.97

Isoelectric Point (pI)

36.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DAGAT PF03982 51 - 300 5.6e-68 Diacylglycerol acyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 705
AclWI GGATC 1 cut(s) 320
AcoI YGGCCR 1 cut(s) 252
AcuI CTGAAG 2 cut(s) 758, 935
AfaI GTAC 4 cut(s) 260, 445, 561, 891
AfiI CCNNNNNNNGG 1 cut(s) 967
AflII CTTAAG 1 cut(s) 617
AflIII ACRYGT 2 cut(s) 84, 316
AgsI TTSAA 4 cut(s) 127, 908, 929, 965
AjnI CCWGG 4 cut(s) 254, 562, 648, 666
AluBI AGCT 3 cut(s) 116, 752, 922
AluI AGCT 3 cut(s) 116, 752, 922
Alw21I GWGCWC 1 cut(s) 597
Alw26I GTCTC 3 cut(s) 422, 529, 572
AlwI GGATC 1 cut(s) 320
AoxI GGCC 2 cut(s) 174, 252
ApeKI GCWGC 1 cut(s) 536
AspLEI GCGC 1 cut(s) 338
AspS9I GGNCC 2 cut(s) 433, 792
AsuHPI GGTGA 2 cut(s) 139, 754
AvaII GGWCC 2 cut(s) 433, 792
BaeI ACNNNNGTAYC 2 cut(s) 250, 283
BalI TGGCCA 1 cut(s) 254
BbsI GAAGAC 1 cut(s) 766
Bbv12I GWGCWC 1 cut(s) 597
BbvI GCAGC 1 cut(s) 523
BccI CCATC 1 cut(s) 91
BciT130I CCWGG 4 cut(s) 256, 564, 650, 668
BcoDI GTCTC 3 cut(s) 422, 529, 572
BfaI CTAG 1 cut(s) 747
BfrI CTTAAG 1 cut(s) 617
BisI GCNGC 1 cut(s) 537
BlsI GCNGC 1 cut(s) 538
BmcAI AGTACT 1 cut(s) 445
Bme1390I CCNGG 4 cut(s) 256, 564, 650, 668
Bme18I GGWCC 2 cut(s) 433, 792
BmgT120I GGNCC 2 cut(s) 433, 792
BmiI GGNNCC 3 cut(s) 672, 793, 794
BmrFI CCNGG 4 cut(s) 256, 564, 650, 668
BmrI ACTGGG 3 cut(s) 212, 284, 414
BmuI ACTGGG 3 cut(s) 212, 284, 414
BpiI GAAGAC 1 cut(s) 766
BpmI CTGGAG 1 cut(s) 671
BpuEI CTTGAG 1 cut(s) 833
BsaI GGTCTC 1 cut(s) 422
BsaJI CCNNGG 4 cut(s) 171, 648, 666, 870
Bsc4I CCNNNNNNNGG 1 cut(s) 967
Bse1I ACTGG 4 cut(s) 218, 290, 409, 674
BseBI CCWGG 4 cut(s) 256, 564, 650, 668
BseDI CCNNGG 4 cut(s) 171, 648, 666, 870
BseGI GGATG 1 cut(s) 811
BseLI CCNNNNNNNGG 1 cut(s) 967
BseNI ACTGG 4 cut(s) 218, 290, 409, 674
BseXI GCAGC 1 cut(s) 523
BsgI GTGCAG 1 cut(s) 593
BshFI GGCC 2 cut(s) 176, 254
BsiHKAI GWGCWC 1 cut(s) 597
BslFI GGGAC 2 cut(s) 200, 778
BslI CCNNNNNNNGG 1 cut(s) 967
BsmAI GTCTC 3 cut(s) 422, 529, 572
BsmFI GGGAC 2 cut(s) 200, 778
BsnI GGCC 2 cut(s) 176, 254
Bso31I GGTCTC 1 cut(s) 422
Bsp1286I GDGCHC 1 cut(s) 597
Bsp143I GATC 1 cut(s) 325
Bsp19I CCATGG 1 cut(s) 870
BspANI GGCC 2 cut(s) 176, 254
BspLI GGNNCC 3 cut(s) 672, 793, 794
BspPI GGATC 1 cut(s) 320
BspTI CTTAAG 1 cut(s) 617
BspTNI GGTCTC 1 cut(s) 422
BsrI ACTGG 4 cut(s) 218, 290, 409, 674
BssECI CCNNGG 4 cut(s) 171, 648, 666, 870
BssMI GATC 1 cut(s) 325
BssT1I CCWWGG 2 cut(s) 171, 870
Bst2UI CCWGG 4 cut(s) 256, 564, 650, 668
Bst4CI ACNGT 2 cut(s) 151, 599
Bst6I CTCTTC 4 cut(s) 18, 241, 622, 870
BstAFI CTTAAG 1 cut(s) 617
BstAPI GCANNNNNTGC 1 cut(s) 446
BstDEI CTNAG 2 cut(s) 423, 470
BstDSI CCRYGG 1 cut(s) 870
BstF5I GGATG 1 cut(s) 811
BstHHI GCGC 1 cut(s) 338
BstKTI GATC 1 cut(s) 328
BstMAI GTCTC 3 cut(s) 422, 529, 572
BstMBI GATC 1 cut(s) 325
BstMWI GCNNNNNNNGC 1 cut(s) 446
BstNI CCWGG 4 cut(s) 256, 564, 650, 668
BstNSI RCATGY 4 cut(s) 88, 279, 320, 824
BstSCI CCNGG 4 cut(s) 254, 562, 648, 666
BstV1I GCAGC 1 cut(s) 523
BstV2I GAAGAC 1 cut(s) 766
BsuRI GGCC 2 cut(s) 176, 254
BtgI CCRYGG 1 cut(s) 870
BtgZI GCGATG 1 cut(s) 110
BtsCI GGATG 1 cut(s) 811
BtsIMutI CAGTG 2 cut(s) 297, 911
CfoI GCGC 1 cut(s) 338
Cfr13I GGNCC 2 cut(s) 433, 792
CseI GACGC 1 cut(s) 541
CsiI ACCWGGT 1 cut(s) 562
Csp6I GTAC 4 cut(s) 259, 444, 560, 890
CspCI CAANNNNNGTGG 2 cut(s) 804, 839
CviAII CATG 9 cut(s) 85, 276, 317, 400, 486, 821, 871, 883, 893
CviQI GTAC 4 cut(s) 259, 444, 560, 890
DdeI CTNAG 2 cut(s) 423, 470
DpnI GATC 1 cut(s) 327
DpnII GATC 1 cut(s) 325
EaeI YGGCCR 1 cut(s) 252
Eam1104I CTCTTC 4 cut(s) 18, 241, 622, 870
EarI CTCTTC 4 cut(s) 18, 241, 622, 870
Eco130I CCWWGG 2 cut(s) 171, 870
Eco31I GGTCTC 1 cut(s) 422
Eco47I GGWCC 2 cut(s) 433, 792
Eco57I CTGAAG 2 cut(s) 758, 935
EcoO109I RGGNCCY 2 cut(s) 433, 792
EcoRII CCWGG 4 cut(s) 254, 562, 648, 666
EcoT14I CCWWGG 2 cut(s) 171, 870
EcoT22I ATGCAT 2 cut(s) 277, 822
ErhI CCWWGG 2 cut(s) 171, 870
FaeI CATG 9 cut(s) 88, 279, 320, 403, 489, 824, 874, 886, 896
FalI AAGNNNNNCTT 2 cut(s) 600, 632
FaqI GGGAC 2 cut(s) 200, 778
FatI CATG 9 cut(s) 84, 275, 316, 399, 485, 820, 870, 882, 892
FauNDI CATATG 1 cut(s) 263
Fnu4HI GCNGC 1 cut(s) 537
FokI GGATG 1 cut(s) 798
Fsp4HI GCNGC 1 cut(s) 537
FspBI CTAG 1 cut(s) 747
GlaI GCGC 1 cut(s) 337
GluI GCNGC 1 cut(s) 537
GsuI CTGGAG 1 cut(s) 671
HaeIII GGCC 2 cut(s) 176, 254
HgaI GACGC 1 cut(s) 541
HhaI GCGC 1 cut(s) 338
Hin1II CATG 9 cut(s) 88, 279, 320, 403, 489, 824, 874, 886, 896
Hin6I GCGC 1 cut(s) 336
HinP1I GCGC 1 cut(s) 336
HincII GTYRAC 1 cut(s) 82
HindII GTYRAC 1 cut(s) 82
HinfI GANTC 1 cut(s) 75
HphI GGTGA 2 cut(s) 139, 754
Hpy166II GTNNAC 3 cut(s) 82, 762, 836
Hpy188I TCNGA 1 cut(s) 809
Hpy188III TCNNGA 5 cut(s) 624, 736, 850, 860, 956
Hpy8I GTNNAC 3 cut(s) 82, 762, 836
HpyAV CCTTC 4 cut(s) 22, 170, 424, 910
HpyCH4III ACNGT 2 cut(s) 151, 599
HpyCH4IV ACGT 1 cut(s) 129
HpyCH4V TGCA 5 cut(s) 275, 389, 440, 574, 820
HpyF10VI GCNNNNNNNGC 1 cut(s) 446
HpyF3I CTNAG 2 cut(s) 423, 470
HpySE526I ACGT 1 cut(s) 129
Hsp92II CATG 9 cut(s) 88, 279, 320, 403, 489, 824, 874, 886, 896
HspAI GCGC 1 cut(s) 336
KflI GGGWCCC 1 cut(s) 792
Kzo9I GATC 1 cut(s) 325
LmnI GCTCC 3 cut(s) 113, 592, 919
Lsp1109I GCAGC 1 cut(s) 523
MabI ACCWGGT 1 cut(s) 562
MaeI CTAG 1 cut(s) 747
MaeII ACGT 1 cut(s) 129
MaeIII GTNAC 3 cut(s) 37, 292, 350
MalI GATC 1 cut(s) 327
MboI GATC 1 cut(s) 325
MboII GAAGA 7 cut(s) 32, 35, 255, 258, 639, 766, 887
MfeI CAATTG 2 cut(s) 331, 375
MhlI GDGCHC 1 cut(s) 597
MlsI TGGCCA 1 cut(s) 254
MluCI AATT 4 cut(s) 331, 375, 517, 965
MluNI TGGCCA 1 cut(s) 254
MmeI TCCRAC 1 cut(s) 552
MnlI CCTC 6 cut(s) 25, 52, 155, 301, 623, 871
Mox20I TGGCCA 1 cut(s) 254
Mph1103I ATGCAT 2 cut(s) 277, 822
MscI TGGCCA 1 cut(s) 254
MseI TTAA 1 cut(s) 618
MslI CAYNNNNRTG 1 cut(s) 89
Msp20I TGGCCA 1 cut(s) 254
MspCI CTTAAG 1 cut(s) 617
MspR9I CCNGG 4 cut(s) 256, 564, 650, 668
MunI CAATTG 2 cut(s) 331, 375
MvaI CCWGG 4 cut(s) 256, 564, 650, 668
MwoI GCNNNNNNNGC 1 cut(s) 446
NcoI CCATGG 1 cut(s) 870
NdeI CATATG 1 cut(s) 263
NdeII GATC 1 cut(s) 325
NlaIII CATG 9 cut(s) 88, 279, 320, 403, 489, 824, 874, 886, 896
NlaIV GGNNCC 3 cut(s) 672, 793, 794
NmuCI GTSAC 2 cut(s) 37, 292
NsiI ATGCAT 2 cut(s) 277, 822
NspI RCATGY 4 cut(s) 88, 279, 320, 824
PciI ACATGT 2 cut(s) 84, 316
PfeI GAWTC 1 cut(s) 75
PkrI GCNGC 1 cut(s) 538
PpuMI RGGWCCY 2 cut(s) 433, 792
PscI ACATGT 2 cut(s) 84, 316
PsiI TTATAA 1 cut(s) 705
Psp5II RGGWCCY 2 cut(s) 433, 792
Psp6I CCWGG 4 cut(s) 254, 562, 648, 666
PspGI CCWGG 4 cut(s) 254, 562, 648, 666
PspN4I GGNNCC 3 cut(s) 672, 793, 794
PspPI GGNCC 2 cut(s) 433, 792
PspPPI RGGWCCY 2 cut(s) 433, 792
RsaI GTAC 4 cut(s) 260, 445, 561, 891
RsaNI GTAC 4 cut(s) 259, 444, 560, 890
RseI CAYNNNNRTG 1 cut(s) 89
SaqAI TTAA 1 cut(s) 618
SatI GCNGC 1 cut(s) 537
Sau3AI GATC 1 cut(s) 325
Sau96I GGNCC 2 cut(s) 433, 792
ScaI AGTACT 1 cut(s) 445
ScrFI CCNGG 4 cut(s) 256, 564, 650, 668
SduI GDGCHC 1 cut(s) 597
SexAI ACCWGGT 1 cut(s) 562
SinI GGWCC 2 cut(s) 433, 792
SmiMI CAYNNNNRTG 1 cut(s) 89
SmlI CTYRAG 2 cut(s) 617, 848
SmoI CTYRAG 2 cut(s) 617, 848
Sse9I AATT 4 cut(s) 331, 375, 517, 965
SspMI CTAG 1 cut(s) 747
StyD4I CCNGG 4 cut(s) 254, 562, 648, 666
StyI CCWWGG 2 cut(s) 171, 870
TaaI ACNGT 2 cut(s) 151, 599
TaiI ACGT 1 cut(s) 132
TaqI TCGA 1 cut(s) 623
TasI AATT 4 cut(s) 331, 375, 517, 965
TatI WGTACW 2 cut(s) 443, 889
TfiI GAWTC 1 cut(s) 75
Tru1I TTAA 1 cut(s) 618
Tru9I TTAA 1 cut(s) 618
TscAI CASTG 2 cut(s) 297, 918
TseFI GTSAC 2 cut(s) 37, 292
TseI GCWGC 1 cut(s) 536
Tsp45I GTSAC 2 cut(s) 37, 292
TspDTI ATGAA 3 cut(s) 26, 110, 443
TspRI CASTG 2 cut(s) 297, 918
Vha464I CTTAAG 1 cut(s) 617
VpaK11BI GGWCC 2 cut(s) 433, 792
XceI RCATGY 4 cut(s) 88, 279, 320, 824
XcmI CCANNNNNNNNNTGG 1 cut(s) 178
XspI CTAG 1 cut(s) 747
ZrmI AGTACT 1 cut(s) 445
Zsp2I ATGCAT 2 cut(s) 277, 822
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.