RchiOBHm_Chr2g0096691

Diacylglycerol O-acyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0006124
Physical Location & Seq
Forward (+)
9398156 .. 9401998
3843 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ47159

Sequence Viewer

Length: 981 bp
ATGGTGGAGATGAAGCAGGAAGAAGAGAAGGAGGGAATGACGATAATCAAAGGAAAAGAAGCTATCGGATTGAGAATCGTGAACATGTTTGTGGCGATGGGTTTGTGGCTTGGAGCTATTCATTTCAACGTGGCTTTGATTCTCTTCACTGTTTGCTTCCTTCCTATTTCCAAGGCCATATTGGTATTTGGGTTGCTTGTGTTGTTTATGATAATCCCAGTTGATGATAAGAGCAAAATAGGAAGAAAAGTGGCCAGGTACATATGTAAGTATGCCTGTGGTTATTTTCCAGTGACTTTGTATGTAGAGGAAAAACACGTCTTTGATCCTAATTGCTCTTATGTCTTTGGGTATGAACCACATTCAGTTTTGCCAATTGGTGTAGTTGCACTGGCTGACATGACTGGGTTCTTGGGTCTCCCTAAGATGAAGGTCCTTGCCAGTACCGCTGTGTTCTATACACCATTCCTAAGGCATATATGGACGTGGTTGGGTCTTACACCAGCCACAAAGAGTAATTTTATCTCCAACTTGGCCGCTGGTTATAGTTGCGTCATTGTGCCTGGTGGAGTGCGGGAGACATTTCTTATGGAGCATGGTAAAGAGAAAGTATTCCTTAGTACAAGAAGAGGATTTGTTCGCATAGCCATGGAGAATGGTTATCCCCTGGTTCCAGTTTTCTGCTTTGGTCAGTCAAATGTTTATAAGTGGTGGAAGCCAAGTGGGGAGTTATTCCTGAAGTTTGCTAGAGCTATCAGATTCACCCCTGTTGTCTTCTGGGGAATGTTTGGGTCCCCTTTACCCTACAGACATCCAATGCATGTGGTAGTAGGTAAACCAATAGCTCTCAAGCAAAATCCAGAACCCACCATGGAAGAGGTCATTGAAGTACATGGTCAGTATGTTGAAGCACTAAAGGATCTTTTTGAAAAGCACAAGGCACGGGCTGGTTTTCCTGATCTTCAATTGGAAATTCTTTGA

Protein Analysis

326

Amino Acids

36.85

Weight (kDa)

9.08

Isoelectric Point (pI)

36.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DAGAT PF03982 51 - 324 2.8e-78 Diacylglycerol acyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 705
AciI CCGC 3 cut(s) 447, 537, 574
AclWI GGATC 2 cut(s) 320, 927
AcoI YGGCCR 2 cut(s) 252, 534
AcsI RAATTY 1 cut(s) 972
AcuI CTGAAG 1 cut(s) 758
AfaI GTAC 4 cut(s) 260, 445, 622, 891
AflIII ACRYGT 2 cut(s) 84, 316
AgsI TTSAA 5 cut(s) 127, 887, 908, 929, 965
AjiI CACGTC 2 cut(s) 319, 486
AjnI CCWGG 3 cut(s) 254, 562, 666
AluBI AGCT 4 cut(s) 62, 116, 752, 845
AluI AGCT 4 cut(s) 62, 116, 752, 845
Alw26I GTCTC 2 cut(s) 422, 572
AlwI GGATC 2 cut(s) 320, 927
AoxI GGCC 3 cut(s) 174, 252, 534
ApoI RAATTY 1 cut(s) 972
Asp700I GAANNNNTTC 1 cut(s) 611
AspS9I GGNCC 2 cut(s) 433, 792
AsuHPI GGTGA 1 cut(s) 754
AvaII GGWCC 2 cut(s) 433, 792
AxyI CCTNAGG 1 cut(s) 470
BaeI ACNNNNGTAYC 2 cut(s) 250, 283
BalI TGGCCA 1 cut(s) 254
BbsI GAAGAC 1 cut(s) 766
BccI CCATC 1 cut(s) 91
BciT130I CCWGG 3 cut(s) 256, 564, 668
BcoDI GTCTC 2 cut(s) 422, 572
BfaI CTAG 1 cut(s) 747
BfmI CTRYAG 1 cut(s) 805
BisI GCNGC 1 cut(s) 537
BlsI GCNGC 1 cut(s) 538
Bme1390I CCNGG 3 cut(s) 256, 564, 668
Bme18I GGWCC 2 cut(s) 433, 792
BmgBI CACGTC 2 cut(s) 319, 486
BmgT120I GGNCC 2 cut(s) 433, 792
BmiI GGNNCC 3 cut(s) 672, 793, 794
BmrFI CCNGG 3 cut(s) 256, 564, 668
BmrI ACTGGG 2 cut(s) 212, 414
BmuI ACTGGG 2 cut(s) 212, 414
BpiI GAAGAC 1 cut(s) 766
BpuEI CTTGAG 1 cut(s) 833
BsaI GGTCTC 1 cut(s) 422
BsaJI CCNNGG 4 cut(s) 171, 648, 666, 870
Bse1I ACTGG 6 cut(s) 218, 290, 396, 409, 441, 674
Bse21I CCTNAGG 1 cut(s) 470
BseBI CCWGG 3 cut(s) 256, 564, 668
BseDI CCNNGG 4 cut(s) 171, 648, 666, 870
BseGI GGATG 1 cut(s) 811
BseNI ACTGG 6 cut(s) 218, 290, 396, 409, 441, 674
BshFI GGCC 3 cut(s) 176, 254, 536
BslFI GGGAC 1 cut(s) 778
BsmAI GTCTC 2 cut(s) 422, 572
BsmFI GGGAC 1 cut(s) 778
BsnI GGCC 3 cut(s) 176, 254, 536
Bso31I GGTCTC 1 cut(s) 422
Bsp143I GATC 3 cut(s) 325, 919, 958
Bsp19I CCATGG 2 cut(s) 648, 870
BspACI CCGC 3 cut(s) 447, 537, 574
BspANI GGCC 3 cut(s) 176, 254, 536
BspLI GGNNCC 3 cut(s) 672, 793, 794
BspPI GGATC 2 cut(s) 320, 927
BspTNI GGTCTC 1 cut(s) 422
BsrI ACTGG 6 cut(s) 218, 290, 396, 409, 441, 674
BssECI CCNNGG 4 cut(s) 171, 648, 666, 870
BssMI GATC 3 cut(s) 325, 919, 958
BssT1I CCWWGG 3 cut(s) 171, 648, 870
Bst2UI CCWGG 3 cut(s) 256, 564, 668
Bst4CI ACNGT 1 cut(s) 151
Bst6I CTCTTC 4 cut(s) 18, 149, 622, 870
BstDEI CTNAG 3 cut(s) 423, 470, 617
BstDSI CCRYGG 2 cut(s) 648, 870
BstF5I GGATG 1 cut(s) 811
BstKTI GATC 3 cut(s) 328, 922, 961
BstMAI GTCTC 2 cut(s) 422, 572
BstMBI GATC 3 cut(s) 325, 919, 958
BstMWI GCNNNNNNNGC 1 cut(s) 446
BstNI CCWGG 3 cut(s) 256, 564, 668
BstNSI RCATGY 2 cut(s) 88, 824
BstSCI CCNGG 3 cut(s) 254, 562, 666
BstSFI CTRYAG 1 cut(s) 805
BstV2I GAAGAC 1 cut(s) 766
BstX2I RGATCY 1 cut(s) 919
BstYI RGATCY 1 cut(s) 919
Bsu36I CCTNAGG 1 cut(s) 470
BsuRI GGCC 3 cut(s) 176, 254, 536
BtgI CCRYGG 2 cut(s) 648, 870
BtgZI GCGATG 1 cut(s) 110
BtrI CACGTC 2 cut(s) 319, 486
BtsCI GGATG 1 cut(s) 811
BtsIMutI CAGTG 3 cut(s) 147, 297, 389
Cfr13I GGNCC 2 cut(s) 433, 792
CseI GACGC 1 cut(s) 541
Csp6I GTAC 4 cut(s) 259, 444, 621, 890
CspCI CAANNNNNGTGG 2 cut(s) 804, 839
CviAII CATG 7 cut(s) 85, 400, 596, 649, 821, 871, 893
CviQI GTAC 4 cut(s) 259, 444, 621, 890
DdeI CTNAG 3 cut(s) 423, 470, 617
DpnI GATC 3 cut(s) 327, 921, 960
DpnII GATC 3 cut(s) 325, 919, 958
EaeI YGGCCR 2 cut(s) 252, 534
Eam1104I CTCTTC 4 cut(s) 18, 149, 622, 870
EarI CTCTTC 4 cut(s) 18, 149, 622, 870
Eco130I CCWWGG 3 cut(s) 171, 648, 870
Eco31I GGTCTC 1 cut(s) 422
Eco47I GGWCC 2 cut(s) 433, 792
Eco57I CTGAAG 1 cut(s) 758
Eco81I CCTNAGG 1 cut(s) 470
EcoO109I RGGNCCY 2 cut(s) 433, 792
EcoRII CCWGG 3 cut(s) 254, 562, 666
EcoT14I CCWWGG 3 cut(s) 171, 648, 870
EcoT22I ATGCAT 1 cut(s) 822
ErhI CCWWGG 3 cut(s) 171, 648, 870
FaeI CATG 7 cut(s) 88, 403, 599, 652, 824, 874, 896
FalI AAGNNNNNCTT 2 cut(s) 600, 632
FaqI GGGAC 1 cut(s) 778
FatI CATG 7 cut(s) 84, 399, 595, 648, 820, 870, 892
FauI CCCGC 1 cut(s) 567
FauNDI CATATG 1 cut(s) 263
Fnu4HI GCNGC 1 cut(s) 537
FokI GGATG 1 cut(s) 798
Fsp4HI GCNGC 1 cut(s) 537
FspBI CTAG 1 cut(s) 747
GluI GCNGC 1 cut(s) 537
HaeIII GGCC 3 cut(s) 176, 254, 536
HgaI GACGC 1 cut(s) 541
Hin1II CATG 7 cut(s) 88, 403, 599, 652, 824, 874, 896
HinfI GANTC 3 cut(s) 75, 139, 759
HphI GGTGA 1 cut(s) 754
Hpy166II GTNNAC 2 cut(s) 82, 836
Hpy188I TCNGA 2 cut(s) 68, 758
Hpy188III TCNNGA 4 cut(s) 79, 736, 860, 956
Hpy8I GTNNAC 2 cut(s) 82, 836
HpyAV CCTTC 3 cut(s) 22, 170, 424
HpyCH4III ACNGT 1 cut(s) 151
HpyCH4IV ACGT 3 cut(s) 129, 318, 485
HpyCH4V TGCA 2 cut(s) 389, 820
HpyF10VI GCNNNNNNNGC 1 cut(s) 446
HpyF3I CTNAG 3 cut(s) 423, 470, 617
HpySE526I ACGT 3 cut(s) 129, 318, 485
Hsp92II CATG 7 cut(s) 88, 403, 599, 652, 824, 874, 896
KflI GGGWCCC 1 cut(s) 792
Kzo9I GATC 3 cut(s) 325, 919, 958
LmnI GCTCC 2 cut(s) 113, 592
MaeI CTAG 1 cut(s) 747
MaeII ACGT 3 cut(s) 129, 318, 485
MaeIII GTNAC 1 cut(s) 292
MalI GATC 3 cut(s) 327, 921, 960
MboI GATC 3 cut(s) 325, 919, 958
MboII GAAGA 8 cut(s) 32, 35, 136, 255, 639, 766, 887, 953
MfeI CAATTG 2 cut(s) 375, 965
MflI RGATCY 1 cut(s) 919
MlsI TGGCCA 1 cut(s) 254
MluCI AATT 5 cut(s) 331, 375, 517, 965, 972
MluNI TGGCCA 1 cut(s) 254
MmeI TCCRAC 1 cut(s) 552
MnlI CCTC 4 cut(s) 25, 301, 623, 871
Mox20I TGGCCA 1 cut(s) 254
Mph1103I ATGCAT 1 cut(s) 822
MroXI GAANNNNTTC 1 cut(s) 611
MscI TGGCCA 1 cut(s) 254
MslI CAYNNNNRTG 2 cut(s) 89, 647
Msp20I TGGCCA 1 cut(s) 254
MspA1I CMGCKG 2 cut(s) 449, 539
MspR9I CCNGG 3 cut(s) 256, 564, 668
MunI CAATTG 2 cut(s) 375, 965
MvaI CCWGG 3 cut(s) 256, 564, 668
MwoI GCNNNNNNNGC 1 cut(s) 446
NcoI CCATGG 2 cut(s) 648, 870
NdeI CATATG 1 cut(s) 263
NdeII GATC 3 cut(s) 325, 919, 958
NlaIII CATG 7 cut(s) 88, 403, 599, 652, 824, 874, 896
NlaIV GGNNCC 3 cut(s) 672, 793, 794
NmuCI GTSAC 1 cut(s) 292
NsiI ATGCAT 1 cut(s) 822
NspI RCATGY 2 cut(s) 88, 824
PciI ACATGT 1 cut(s) 84
PdmI GAANNNNTTC 1 cut(s) 611
PfeI GAWTC 3 cut(s) 75, 139, 759
PkrI GCNGC 1 cut(s) 538
PpuMI RGGWCCY 2 cut(s) 433, 792
PscI ACATGT 1 cut(s) 84
PsiI TTATAA 1 cut(s) 705
Psp5II RGGWCCY 2 cut(s) 433, 792
Psp6I CCWGG 3 cut(s) 254, 562, 666
PspGI CCWGG 3 cut(s) 254, 562, 666
PspN4I GGNNCC 3 cut(s) 672, 793, 794
PspPI GGNCC 2 cut(s) 433, 792
PspPPI RGGWCCY 2 cut(s) 433, 792
PsuI RGATCY 1 cut(s) 919
RsaI GTAC 4 cut(s) 260, 445, 622, 891
RsaNI GTAC 4 cut(s) 259, 444, 621, 890
RseI CAYNNNNRTG 2 cut(s) 89, 647
SatI GCNGC 1 cut(s) 537
Sau3AI GATC 3 cut(s) 325, 919, 958
Sau96I GGNCC 2 cut(s) 433, 792
ScrFI CCNGG 3 cut(s) 256, 564, 668
SfcI CTRYAG 1 cut(s) 805
SinI GGWCC 2 cut(s) 433, 792
SmiMI CAYNNNNRTG 2 cut(s) 89, 647
SmlI CTYRAG 1 cut(s) 848
SmoI CTYRAG 1 cut(s) 848
Sse9I AATT 5 cut(s) 331, 375, 517, 965, 972
SsiI CCGC 3 cut(s) 447, 537, 574
SspMI CTAG 1 cut(s) 747
StyD4I CCNGG 3 cut(s) 254, 562, 666
StyI CCWWGG 3 cut(s) 171, 648, 870
TaaI ACNGT 1 cut(s) 151
TaiI ACGT 3 cut(s) 132, 321, 488
TasI AATT 5 cut(s) 331, 375, 517, 965, 972
TatI WGTACW 2 cut(s) 620, 889
TauI GCSGC 1 cut(s) 539
TfiI GAWTC 3 cut(s) 75, 139, 759
TscAI CASTG 3 cut(s) 154, 297, 396
TseFI GTSAC 1 cut(s) 292
Tsp45I GTSAC 1 cut(s) 292
TspDTI ATGAA 4 cut(s) 26, 110, 369, 443
TspRI CASTG 3 cut(s) 154, 297, 396
VpaK11BI GGWCC 2 cut(s) 433, 792
XapI RAATTY 1 cut(s) 972
XceI RCATGY 2 cut(s) 88, 824
XcmI CCANNNNNNNNNTGG 1 cut(s) 178
XmnI GAANNNNTTC 1 cut(s) 611
XspI CTAG 1 cut(s) 747
Zsp2I ATGCAT 1 cut(s) 822
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.