Rh2AG107100

Diacylglycerol O-acyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0006124
Physical Location & Seq
Forward (+)
9404655 .. 9408608
3954 bp
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UTR
Exon/CDS
Intron
Rh2AG107100.1

Sequence Viewer

Length: 831 bp
ATGGTGGAGATGAAGCAGGAAGAAGAGAAGGAGGGAATGACGATAATCAAAGGAAAAGAAGCTATCGGATTGAGAATCGTGAACATGTTTGTGGCGATGGGTTTGTGGCTTGGAGCTATTCATTTCAACGTGGCTTTGATTCTCTTCACTGTTTGCTTCCTTCCAATTTCCAAGGCCATATTGGTATTTGGGTTGCTTGTGTTGTTTATGATAATCCCAGTTGATGATAAGAGCAAAATAGGAAGAAAAGTGGCCAGGTACATATGTAAGTATGCCTGTGGTTATTTTCCAGTGACTTTGTATGTAGAGGAAAAACACGTCTTTGATCCTAATTGCTCTTATGTCTTTGGGTATGAACCACATTCAGTTTTGCCAATTGGTGTAGTTGCACTGGCTGACATGACTGGGTTCTTGGGTCTCCCTAAGATGAAGGTCCTTGCGAGTACTGCTGTGTTCTATACACCATTCCTAAGGCATATATGGACGTGGTTGGGTCTTACACCAGCCACAAAGAGTAATTTTATCTCCAACTTGGCCGCTGGTTATAGTTGCGTCATTGTGCCTGGTGGAGTGCGGGAGACATTTCTTATGGAGCATGGTAAAGAGAAAGTATTCCTTAGTACAAGAAGAGGATTTGTTCGCATAGCCATGGAGAATGGTTATCCCCTTGTTCCAGTTTTCTGCTTTGGTCAGTCAAATGTTTATAAGTGGTGGAAGCCAAGTGGGGAGTTATTCCTGAAGTTTGCTAGAGCTATCAGATTCACCCCTGTTGTCTTCTGGGGAATGTTCGGGTATGTATTTAGTTGTTTGATATTTATTATTTGCTTTTAA

Protein Analysis

276

Amino Acids

31.23

Weight (kDa)

9.21

Isoelectric Point (pI)

32.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DAGAT PF03982 51 - 267 6e-56 Diacylglycerol acyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 705
AciI CCGC 2 cut(s) 537, 574
AclWI GGATC 1 cut(s) 320
AcoI YGGCCR 2 cut(s) 252, 534
AcuI CTGAAG 1 cut(s) 758
AfaI GTAC 3 cut(s) 260, 445, 622
AflIII ACRYGT 2 cut(s) 84, 316
AgsI TTSAA 1 cut(s) 127
AjiI CACGTC 2 cut(s) 319, 486
AjnI CCWGG 2 cut(s) 254, 562
AluBI AGCT 3 cut(s) 62, 116, 752
AluI AGCT 3 cut(s) 62, 116, 752
Alw26I GTCTC 2 cut(s) 422, 572
AlwI GGATC 1 cut(s) 320
AoxI GGCC 3 cut(s) 174, 252, 534
Asp700I GAANNNNTTC 1 cut(s) 611
AspS9I GGNCC 1 cut(s) 433
AsuHPI GGTGA 1 cut(s) 754
AvaII GGWCC 1 cut(s) 433
AxyI CCTNAGG 1 cut(s) 470
BaeI ACNNNNGTAYC 2 cut(s) 250, 283
BalI TGGCCA 1 cut(s) 254
BbsI GAAGAC 1 cut(s) 766
BccI CCATC 1 cut(s) 91
BciT130I CCWGG 2 cut(s) 256, 564
BcoDI GTCTC 2 cut(s) 422, 572
BfaI CTAG 1 cut(s) 747
BisI GCNGC 1 cut(s) 537
BlsI GCNGC 1 cut(s) 538
BmcAI AGTACT 1 cut(s) 445
Bme1390I CCNGG 2 cut(s) 256, 564
Bme18I GGWCC 1 cut(s) 433
BmgBI CACGTC 2 cut(s) 319, 486
BmgT120I GGNCC 1 cut(s) 433
BmrFI CCNGG 2 cut(s) 256, 564
BmrI ACTGGG 2 cut(s) 212, 414
BmuI ACTGGG 2 cut(s) 212, 414
BpiI GAAGAC 1 cut(s) 766
BsaI GGTCTC 1 cut(s) 422
BsaJI CCNNGG 2 cut(s) 171, 648
Bse1I ACTGG 5 cut(s) 218, 290, 396, 409, 674
Bse21I CCTNAGG 1 cut(s) 470
BseBI CCWGG 2 cut(s) 256, 564
BseDI CCNNGG 2 cut(s) 171, 648
BseNI ACTGG 5 cut(s) 218, 290, 396, 409, 674
BshFI GGCC 3 cut(s) 176, 254, 536
BsmAI GTCTC 2 cut(s) 422, 572
BsnI GGCC 3 cut(s) 176, 254, 536
Bso31I GGTCTC 1 cut(s) 422
Bsp143I GATC 1 cut(s) 325
Bsp19I CCATGG 1 cut(s) 648
BspACI CCGC 2 cut(s) 537, 574
BspANI GGCC 3 cut(s) 176, 254, 536
BspPI GGATC 1 cut(s) 320
BspTNI GGTCTC 1 cut(s) 422
BsrI ACTGG 5 cut(s) 218, 290, 396, 409, 674
BssECI CCNNGG 2 cut(s) 171, 648
BssMI GATC 1 cut(s) 325
BssT1I CCWWGG 2 cut(s) 171, 648
Bst2UI CCWGG 2 cut(s) 256, 564
Bst4CI ACNGT 1 cut(s) 151
Bst6I CTCTTC 3 cut(s) 18, 149, 622
BstDEI CTNAG 3 cut(s) 423, 470, 617
BstDSI CCRYGG 1 cut(s) 648
BstKTI GATC 1 cut(s) 328
BstMAI GTCTC 2 cut(s) 422, 572
BstMBI GATC 1 cut(s) 325
BstMWI GCNNNNNNNGC 1 cut(s) 446
BstNI CCWGG 2 cut(s) 256, 564
BstNSI RCATGY 1 cut(s) 88
BstSCI CCNGG 2 cut(s) 254, 562
BstV2I GAAGAC 1 cut(s) 766
Bsu36I CCTNAGG 1 cut(s) 470
BsuRI GGCC 3 cut(s) 176, 254, 536
BtgI CCRYGG 1 cut(s) 648
BtgZI GCGATG 1 cut(s) 110
BtrI CACGTC 2 cut(s) 319, 486
BtsIMutI CAGTG 3 cut(s) 147, 297, 389
Cfr13I GGNCC 1 cut(s) 433
CseI GACGC 1 cut(s) 541
Csp6I GTAC 3 cut(s) 259, 444, 621
CviAII CATG 4 cut(s) 85, 400, 596, 649
CviQI GTAC 3 cut(s) 259, 444, 621
DdeI CTNAG 3 cut(s) 423, 470, 617
DpnI GATC 1 cut(s) 327
DpnII GATC 1 cut(s) 325
EaeI YGGCCR 2 cut(s) 252, 534
Eam1104I CTCTTC 3 cut(s) 18, 149, 622
EarI CTCTTC 3 cut(s) 18, 149, 622
Eco130I CCWWGG 2 cut(s) 171, 648
Eco31I GGTCTC 1 cut(s) 422
Eco47I GGWCC 1 cut(s) 433
Eco57I CTGAAG 1 cut(s) 758
Eco81I CCTNAGG 1 cut(s) 470
EcoO109I RGGNCCY 1 cut(s) 433
EcoRII CCWGG 2 cut(s) 254, 562
EcoT14I CCWWGG 2 cut(s) 171, 648
ErhI CCWWGG 2 cut(s) 171, 648
FaeI CATG 4 cut(s) 88, 403, 599, 652
FalI AAGNNNNNCTT 2 cut(s) 600, 632
FatI CATG 4 cut(s) 84, 399, 595, 648
FauI CCCGC 1 cut(s) 567
FauNDI CATATG 1 cut(s) 263
Fnu4HI GCNGC 1 cut(s) 537
Fsp4HI GCNGC 1 cut(s) 537
FspBI CTAG 1 cut(s) 747
GluI GCNGC 1 cut(s) 537
HaeIII GGCC 3 cut(s) 176, 254, 536
HgaI GACGC 1 cut(s) 541
Hin1II CATG 4 cut(s) 88, 403, 599, 652
HinfI GANTC 3 cut(s) 75, 139, 759
HphI GGTGA 1 cut(s) 754
Hpy166II GTNNAC 1 cut(s) 82
Hpy188I TCNGA 2 cut(s) 68, 758
Hpy188III TCNNGA 2 cut(s) 79, 736
Hpy8I GTNNAC 1 cut(s) 82
HpyAV CCTTC 3 cut(s) 22, 170, 424
HpyCH4III ACNGT 1 cut(s) 151
HpyCH4IV ACGT 3 cut(s) 129, 318, 485
HpyCH4V TGCA 1 cut(s) 389
HpyF10VI GCNNNNNNNGC 1 cut(s) 446
HpyF3I CTNAG 3 cut(s) 423, 470, 617
HpySE526I ACGT 3 cut(s) 129, 318, 485
Hsp92II CATG 4 cut(s) 88, 403, 599, 652
Kzo9I GATC 1 cut(s) 325
LmnI GCTCC 2 cut(s) 113, 592
MaeI CTAG 1 cut(s) 747
MaeII ACGT 3 cut(s) 129, 318, 485
MaeIII GTNAC 1 cut(s) 292
MalI GATC 1 cut(s) 327
MboI GATC 1 cut(s) 325
MboII GAAGA 6 cut(s) 32, 35, 136, 255, 639, 766
MfeI CAATTG 1 cut(s) 375
MlsI TGGCCA 1 cut(s) 254
MluCI AATT 4 cut(s) 165, 331, 375, 517
MluNI TGGCCA 1 cut(s) 254
MmeI TCCRAC 1 cut(s) 552
MnlI CCTC 3 cut(s) 25, 301, 623
Mox20I TGGCCA 1 cut(s) 254
MroXI GAANNNNTTC 1 cut(s) 611
MscI TGGCCA 1 cut(s) 254
MseI TTAA 1 cut(s) 829
MslI CAYNNNNRTG 2 cut(s) 89, 647
Msp20I TGGCCA 1 cut(s) 254
MspA1I CMGCKG 1 cut(s) 539
MspR9I CCNGG 2 cut(s) 256, 564
MunI CAATTG 1 cut(s) 375
MvaI CCWGG 2 cut(s) 256, 564
MwoI GCNNNNNNNGC 1 cut(s) 446
NcoI CCATGG 1 cut(s) 648
NdeI CATATG 1 cut(s) 263
NdeII GATC 1 cut(s) 325
NlaIII CATG 4 cut(s) 88, 403, 599, 652
NmuCI GTSAC 1 cut(s) 292
NspI RCATGY 1 cut(s) 88
PciI ACATGT 1 cut(s) 84
PdmI GAANNNNTTC 1 cut(s) 611
PfeI GAWTC 3 cut(s) 75, 139, 759
PkrI GCNGC 1 cut(s) 538
PpuMI RGGWCCY 1 cut(s) 433
PscI ACATGT 1 cut(s) 84
PsiI TTATAA 1 cut(s) 705
Psp5II RGGWCCY 1 cut(s) 433
Psp6I CCWGG 2 cut(s) 254, 562
PspGI CCWGG 2 cut(s) 254, 562
PspPI GGNCC 1 cut(s) 433
PspPPI RGGWCCY 1 cut(s) 433
RsaI GTAC 3 cut(s) 260, 445, 622
RsaNI GTAC 3 cut(s) 259, 444, 621
RseI CAYNNNNRTG 2 cut(s) 89, 647
SaqAI TTAA 1 cut(s) 829
SatI GCNGC 1 cut(s) 537
Sau3AI GATC 1 cut(s) 325
Sau96I GGNCC 1 cut(s) 433
ScaI AGTACT 1 cut(s) 445
ScrFI CCNGG 2 cut(s) 256, 564
SetI ASST 8 cut(s) 64, 118, 132, 260, 321, 435, 488, 754
SinI GGWCC 1 cut(s) 433
SmiMI CAYNNNNRTG 2 cut(s) 89, 647
Sse9I AATT 4 cut(s) 165, 331, 375, 517
SsiI CCGC 2 cut(s) 537, 574
SspMI CTAG 1 cut(s) 747
StyD4I CCNGG 2 cut(s) 254, 562
StyI CCWWGG 2 cut(s) 171, 648
TaaI ACNGT 1 cut(s) 151
TaiI ACGT 3 cut(s) 132, 321, 488
TasI AATT 4 cut(s) 165, 331, 375, 517
TatI WGTACW 2 cut(s) 443, 620
TauI GCSGC 1 cut(s) 539
TfiI GAWTC 3 cut(s) 75, 139, 759
Tru1I TTAA 1 cut(s) 829
Tru9I TTAA 1 cut(s) 829
TscAI CASTG 3 cut(s) 154, 297, 396
TseFI GTSAC 1 cut(s) 292
Tsp45I GTSAC 1 cut(s) 292
TspDTI ATGAA 4 cut(s) 26, 110, 369, 443
TspRI CASTG 3 cut(s) 154, 297, 396
VpaK11BI GGWCC 1 cut(s) 433
XceI RCATGY 1 cut(s) 88
XcmI CCANNNNNNNNNTGG 1 cut(s) 178
XmnI GAANNNNTTC 1 cut(s) 611
XspI CTAG 1 cut(s) 747
ZrmI AGTACT 1 cut(s) 445
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.