Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0006124

Physical Location & Seq

Genomic Coordinates Forward (+)

8700744 .. 8708878

Gene Structure 8135 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1107700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 690 bp

ATGCCTGTTCTTATTTTCCCGAAAGAGGAAGAGACTGATAGAGAATGTGGGATGTTTGGTTATGAACCACATTCGGTTTTTGCAATTGGTGTTATCGCACTTGCTGACCTTACTGGGTTCTTGCCTCTCACCAAAATCAAGACCCTTGCGAGTAGCGCGGTGTTCTACACACCATTCATGAGACATATATGGACATGGTTGGGTTTTTCACCGGCCACAAGGAGTAATTTTATCTCCCACTTATCCGCCGGTTATAGTTGCGTCATTGTGCCTGGTGGAGTGCGGGAGATATTTTACATGGAGCCTGGCACTGAGGTAGCATTTCTTAAGTCGAGAAGAGGATTTGTTCGCATAGCCATGGAGCTGGGCCATCCCCTGGTTCCAGTTTTCTGCTTTGGTCAGTCAAAGGTATACAAATGGTGGAGGCCAAGTGGGGAATTAGTTTGGAAGTTTGCCAGAGCTCTCAAGTTCTCCCCAGTTTTCTTCTGGGGAATATTTGGAACTCCTGTACCCTACAGTCATCCAATGCACGTGGTGGTCGGAAAACCAATCGATCTCAAGAAAACTCCGCAACCCACCATGGAAGAGGTGAACGAAGTGCACGGTCGGTTTGTTGCAGCACTGCAGGATCTATTCGAAAGGCACAAGGCACGGGTTGGTCACGCCAACCTTCAATTAAAAATTATTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004144 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0005811 GO:0006629 GO:0006638 GO:0006639 GO:0006641 GO:0006996 GO:0008150 GO:0008152 GO:0008374 GO:0008610 GO:0009058 GO:0009987 GO:0012505 GO:0016020 GO:0016043 GO:0016411 GO:0016740 GO:0016746 GO:0016747 GO:0019432 GO:0031984 GO:0034389 GO:0042175 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0045017 GO:0046460 GO:0046463 GO:0046486 GO:0071704 GO:0071840 GO:0098827 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00561 ko04975 map00561 map04975

Pfam Domains

Protein Families

DAGAT

Protein Analysis

230

Amino Acids

25.96

Weight (kDa)

9.4

Isoelectric Point (pI)

50.45

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DAGAT	PF03982	14 - 223	1.9e-60	Diacylglycerol acyltransferase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006124)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G51520
fragaria_vesca	FvH4_1g09760 FvH4_1g09760
malus_domestica	MD02G1107700.v1.1 MD02G1107800.v1.1 MD15G1227200.v1.1
prunus_persica	Prupe.7G188100_v2.0.a1 Prupe.7G188100_v2.0.a1 Prupe.7G188100_v2.0.a1
pyrus_communis	pycom02g08660 pycom15g20210
rosa_chinensis	RchiOBHm_Chr2g0096691
rosa_laevigata	RLG00000016662 RLG00000036260
rosa_multiflora	Rmu_sc0003565.1_g000021
rosa_roxburghii	Rroxscaffold_2G00145410 Rroxscaffold_6G00397690
rosa_rugosa	Rorug02G0059600 Rorug02G0059700 Rorug04G0089100
rosa_samantha	Rh2AG107100 Rh2BG109500 Rh2CG111300 Rh2DG110900 Rh5BG533300 Rh6AG230400
rosa_wichuraiana	Rw2G008300

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	376
AccI	GTMKAC	1 cut(s)	411
AccII	CGCG	1 cut(s)	158
AciI	CCGC	4 cut(s)	158, 246, 283, 569
AclWI	GGATC	1 cut(s)	636
AcoI	YGGCCR	1 cut(s)	213
AcvI	CACGTG	1 cut(s)	532
AdeI	CACNNNGTG	1 cut(s)	535
AfaI	GTAC	1 cut(s)	510
AfiI	CCNNNNNNNGG	2 cut(s)	25, 376
AflII	CTTAAG	1 cut(s)	326
AgsI	TTSAA	1 cut(s)	674
AjnI	CCWGG	3 cut(s)	271, 304, 375
AluBI	AGCT	2 cut(s)	364, 461
AluI	AGCT	2 cut(s)	364, 461
Alw21I	GWGCWC	2 cut(s)	463, 603
Alw26I	GTCTC	2 cut(s)	26, 175
Alw44I	GTGCAC	1 cut(s)	599
AlwI	GGATC	1 cut(s)	636
AoxI	GGCC	3 cut(s)	213, 367, 425
ApaLI	GTGCAC	1 cut(s)	599
ApeKI	GCWGC	1 cut(s)	617
AspLEI	GCGC	1 cut(s)	158
AspS9I	GGNCC	1 cut(s)	367
AsuHPI	GGTGA	3 cut(s)	121, 201, 601
AsuII	TTCGAA	1 cut(s)	636
BaeGI	GKGCMC	1 cut(s)	603
BaeI	ACNNNNGTAYC	2 cut(s)	492, 525
BanII	GRGCYC	1 cut(s)	463
BbrPI	CACGTG	1 cut(s)	532
Bbv12I	GWGCWC	2 cut(s)	463, 603
BbvI	GCAGC	1 cut(s)	629
BccI	CCATC	1 cut(s)	378
BciT130I	CCWGG	3 cut(s)	273, 306, 377
BcoDI	GTCTC	2 cut(s)	26, 175
BfmI	CTRYAG	2 cut(s)	514, 623
BfrI	CTTAAG	1 cut(s)	326
BisI	GCNGC	1 cut(s)	618
BlsI	GCNGC	1 cut(s)	619
Bme1390I	CCNGG	3 cut(s)	273, 306, 377
BmgT120I	GGNCC	1 cut(s)	367
BmiI	GGNNCC	2 cut(s)	303, 381
BmrFI	CCNGG	3 cut(s)	273, 306, 377
BmrI	ACTGGG	2 cut(s)	123, 470
BmuI	ACTGGG	2 cut(s)	123, 470
Bpu14I	TTCGAA	1 cut(s)	636
BpuEI	CTTGAG	2 cut(s)	449, 542
Bsa29I	ATCGAT	1 cut(s)	552
BsaAI	YACGTR	1 cut(s)	532
BsaJI	CCNNGG	3 cut(s)	357, 375, 579
BsaXI	ACNNNNNCTCC	2 cut(s)	415, 445
Bsc4I	CCNNNNNNNGG	2 cut(s)	25, 376
Bse118I	RCCGGY	2 cut(s)	211, 248
Bse1I	ACTGG	3 cut(s)	118, 383, 476
BseBI	CCWGG	3 cut(s)	273, 306, 377
BseCI	ATCGAT	1 cut(s)	552
BseDI	CCNNGG	3 cut(s)	357, 375, 579
BseGI	GGATG	3 cut(s)	57, 370, 520
BseLI	CCNNNNNNNGG	2 cut(s)	25, 376
BseMII	CTCAG	1 cut(s)	303
BseNI	ACTGG	3 cut(s)	118, 383, 476
BseSI	GKGCMC	1 cut(s)	603
BseXI	GCAGC	1 cut(s)	629
BseYI	CCCAGC	1 cut(s)	364
Bsh1236I	CGCG	1 cut(s)	158
Bsh1285I	CGRYCG	1 cut(s)	607
BshFI	GGCC	3 cut(s)	215, 369, 427
BshVI	ATCGAT	1 cut(s)	552
BsiEI	CGRYCG	1 cut(s)	607
BsiHKAI	GWGCWC	2 cut(s)	463, 603
BsiSI	CCGG	2 cut(s)	212, 249
BslI	CCNNNNNNNGG	2 cut(s)	25, 376
BsmAI	GTCTC	2 cut(s)	26, 175
BsnI	GGCC	3 cut(s)	215, 369, 427
Bsp119I	TTCGAA	1 cut(s)	636
Bsp1286I	GDGCHC	2 cut(s)	463, 603
Bsp143I	GATC	2 cut(s)	553, 628
Bsp19I	CCATGG	2 cut(s)	357, 579
BspACI	CCGC	4 cut(s)	158, 246, 283, 569
BspANI	GGCC	3 cut(s)	215, 369, 427
BspCNI	CTCAG	1 cut(s)	304
BspDI	ATCGAT	1 cut(s)	552
BspFNI	CGCG	1 cut(s)	158
BspHI	TCATGA	1 cut(s)	177
BspLI	GGNNCC	2 cut(s)	303, 381
BspMAI	CTGCAG	1 cut(s)	627
BspPI	GGATC	1 cut(s)	636
BspT104I	TTCGAA	1 cut(s)	636
BspTI	CTTAAG	1 cut(s)	326
BsrFI	RCCGGY	2 cut(s)	211, 248
BsrI	ACTGG	3 cut(s)	118, 383, 476
BssAI	RCCGGY	2 cut(s)	211, 248
BssECI	CCNNGG	3 cut(s)	357, 375, 579
BssMI	GATC	2 cut(s)	553, 628
BssNAI	GTATAC	1 cut(s)	412
BssT1I	CCWWGG	2 cut(s)	357, 579
Bst1107I	GTATAC	1 cut(s)	412
Bst2UI	CCWGG	3 cut(s)	273, 306, 377
Bst4CI	ACNGT	2 cut(s)	518, 605
Bst6I	CTCTTC	3 cut(s)	24, 331, 579
BstAFI	CTTAAG	1 cut(s)	326
BstBAI	YACGTR	1 cut(s)	532
BstBI	TTCGAA	1 cut(s)	636
BstDEI	CTNAG	1 cut(s)	312
BstDSI	CCRYGG	2 cut(s)	357, 579
BstF5I	GGATG	3 cut(s)	57, 370, 520
BstFNI	CGCG	1 cut(s)	158
BstHHI	GCGC	1 cut(s)	158
BstKTI	GATC	2 cut(s)	556, 631
BstMAI	GTCTC	2 cut(s)	26, 175
BstMBI	GATC	2 cut(s)	553, 628
BstMCI	CGRYCG	1 cut(s)	607
BstMWI	GCNNNNNNNGC	1 cut(s)	155
BstNI	CCWGG	3 cut(s)	273, 306, 377
BstSCI	CCNGG	3 cut(s)	271, 304, 375
BstSFI	CTRYAG	2 cut(s)	514, 623
BstSLI	GKGCMC	1 cut(s)	603
BstUI	CGCG	1 cut(s)	158
BstV1I	GCAGC	1 cut(s)	629
BstX2I	RGATCY	1 cut(s)	628
BstXI	CCANNNNNNTGG	1 cut(s)	364
BstYI	RGATCY	1 cut(s)	628
BstZ17I	GTATAC	1 cut(s)	412
Bsu15I	ATCGAT	1 cut(s)	552
BsuRI	GGCC	3 cut(s)	215, 369, 427
BsuTUI	ATCGAT	1 cut(s)	552
BtgI	CCRYGG	2 cut(s)	357, 579
BtsCI	GGATG	3 cut(s)	57, 370, 520
BtsI	GCAGTG	1 cut(s)	620
BtsIMutI	CAGTG	2 cut(s)	309, 620
CciI	TCATGA	1 cut(s)	177
CfoI	GCGC	1 cut(s)	158
Cfr10I	RCCGGY	2 cut(s)	211, 248
Cfr13I	GGNCC	1 cut(s)	367
ClaI	ATCGAT	1 cut(s)	552
CseI	GACGC	1 cut(s)	250
Csp6I	GTAC	1 cut(s)	509
CspCI	CAANNNNNGTGG	2 cut(s)	513, 548
CviAII	CATG	5 cut(s)	178, 195, 298, 358, 580
CviJI	RGCY	7 cut(s)	215, 304, 356, 364, 369, 427, 461
CviKI_1	RGCY	7 cut(s)	215, 304, 356, 364, 369, 427, 461
CviQI	GTAC	1 cut(s)	509
DdeI	CTNAG	1 cut(s)	312
DpnI	GATC	2 cut(s)	555, 630
DpnII	GATC	2 cut(s)	553, 628
DraIII	CACNNNGTG	1 cut(s)	535
EaeI	YGGCCR	1 cut(s)	213
Eam1104I	CTCTTC	3 cut(s)	24, 331, 579
EarI	CTCTTC	3 cut(s)	24, 331, 579
EciI	GGCGGA	1 cut(s)	235
Ecl136II	GAGCTC	1 cut(s)	461
Eco130I	CCWWGG	2 cut(s)	357, 579
Eco24I	GRGCYC	1 cut(s)	463
Eco53kI	GAGCTC	1 cut(s)	461
Eco72I	CACGTG	1 cut(s)	532
EcoICRI	GAGCTC	1 cut(s)	461
EcoRII	CCWGG	3 cut(s)	271, 304, 375
EcoT14I	CCWWGG	2 cut(s)	357, 579
EcoT38I	GRGCYC	1 cut(s)	463
ErhI	CCWWGG	2 cut(s)	357, 579
FaeI	CATG	5 cut(s)	181, 198, 301, 361, 583
FatI	CATG	5 cut(s)	177, 194, 297, 357, 579
FauI	CCCGC	1 cut(s)	276
FblI	GTMKAC	1 cut(s)	411
Fnu4HI	GCNGC	1 cut(s)	618
FokI	GGATG	3 cut(s)	64, 357, 507
FriOI	GRGCYC	1 cut(s)	463
Fsp4HI	GCNGC	1 cut(s)	618
GlaI	GCGC	1 cut(s)	157
GluI	GCNGC	1 cut(s)	618
GsaI	CCCAGC	1 cut(s)	368
HaeIII	GGCC	3 cut(s)	215, 369, 427
HapII	CCGG	2 cut(s)	212, 249
HgaI	GACGC	1 cut(s)	250
HhaI	GCGC	1 cut(s)	158
Hin1II	CATG	5 cut(s)	181, 198, 301, 361, 583
Hin6I	GCGC	1 cut(s)	156
HinP1I	GCGC	1 cut(s)	156
HpaII	CCGG	2 cut(s)	212, 249
HphI	GGTGA	3 cut(s)	121, 201, 601
Hpy166II	GTNNAC	3 cut(s)	412, 592, 601
Hpy188I	TCNGA	1 cut(s)	542
Hpy188III	TCNNGA	5 cut(s)	19, 139, 178, 333, 559
Hpy8I	GTNNAC	3 cut(s)	412, 592, 601
HpyAV	CCTTC	1 cut(s)	680
HpyCH4III	ACNGT	2 cut(s)	518, 605
HpyCH4IV	ACGT	1 cut(s)	531
HpyCH4V	TGCA	5 cut(s)	83, 529, 601, 617, 625
HpyF10VI	GCNNNNNNNGC	1 cut(s)	155
HpyF3I	CTNAG	1 cut(s)	312
HpySE526I	ACGT	1 cut(s)	531
Hsp92II	CATG	5 cut(s)	181, 198, 301, 361, 583
HspAI	GCGC	1 cut(s)	156
Kzo9I	GATC	2 cut(s)	553, 628
LmnI	GCTCC	2 cut(s)	301, 361
Lsp1109I	GCAGC	1 cut(s)	629
MaeII	ACGT	1 cut(s)	531
MaeIII	GTNAC	1 cut(s)	659
MalI	GATC	2 cut(s)	555, 630
MboI	GATC	2 cut(s)	553, 628
MboII	GAAGA	4 cut(s)	41, 348, 475, 596
MfeI	CAATTG	1 cut(s)	84
MflI	RGATCY	1 cut(s)	628
MhlI	GDGCHC	2 cut(s)	463, 603
MluCI	AATT	5 cut(s)	84, 226, 437, 674, 681
MmeI	TCCRAC	1 cut(s)	520
MnlI	CCTC	6 cut(s)	19, 135, 307, 332, 417, 580
MseI	TTAA	2 cut(s)	327, 677
MslI	CAYNNNNRTG	1 cut(s)	356
MspCI	CTTAAG	1 cut(s)	326
MspI	CCGG	2 cut(s)	212, 249
MspR9I	CCNGG	3 cut(s)	273, 306, 377
MunI	CAATTG	1 cut(s)	84
MvaI	CCWGG	3 cut(s)	273, 306, 377
MvnI	CGCG	1 cut(s)	158
MwoI	GCNNNNNNNGC	1 cut(s)	155
NcoI	CCATGG	2 cut(s)	357, 579
NdeII	GATC	2 cut(s)	553, 628
NlaIII	CATG	5 cut(s)	181, 198, 301, 361, 583
NlaIV	GGNNCC	2 cut(s)	303, 381
NmuCI	GTSAC	1 cut(s)	659
NspV	TTCGAA	1 cut(s)	636
PagI	TCATGA	1 cut(s)	177
PflMI	CCANNNNNTGG	1 cut(s)	376
PkrI	GCNGC	1 cut(s)	619
PmaCI	CACGTG	1 cut(s)	532
PmlI	CACGTG	1 cut(s)	532
Ppu21I	YACGTR	1 cut(s)	532
Psp124BI	GAGCTC	1 cut(s)	463
Psp6I	CCWGG	3 cut(s)	271, 304, 375
PspCI	CACGTG	1 cut(s)	532
PspFI	CCCAGC	1 cut(s)	364
PspGI	CCWGG	3 cut(s)	271, 304, 375
PspN4I	GGNNCC	2 cut(s)	303, 381
PspPI	GGNCC	1 cut(s)	367
PstI	CTGCAG	1 cut(s)	627
PsuI	RGATCY	1 cut(s)	628
RsaI	GTAC	1 cut(s)	510
RsaNI	GTAC	1 cut(s)	509
RseI	CAYNNNNRTG	1 cut(s)	356
SacI	GAGCTC	1 cut(s)	463
SaqAI	TTAA	2 cut(s)	327, 677
SatI	GCNGC	1 cut(s)	618
Sau3AI	GATC	2 cut(s)	553, 628
Sau96I	GGNCC	1 cut(s)	367
ScrFI	CCNGG	3 cut(s)	273, 306, 377
SduI	GDGCHC	2 cut(s)	463, 603
SetI	ASST	8 cut(s)	111, 318, 366, 411, 463, 534, 591, 672
SfcI	CTRYAG	2 cut(s)	514, 623
SfuI	TTCGAA	1 cut(s)	636
SmiMI	CAYNNNNRTG	1 cut(s)	356
SmlI	CTYRAG	3 cut(s)	326, 464, 557
SmoI	CTYRAG	3 cut(s)	326, 464, 557
Sse9I	AATT	5 cut(s)	84, 226, 437, 674, 681
SsiI	CCGC	4 cut(s)	158, 246, 283, 569
SspI	AATATT	1 cut(s)	495
SstI	GAGCTC	1 cut(s)	463
StyD4I	CCNGG	3 cut(s)	271, 304, 375
StyI	CCWWGG	2 cut(s)	357, 579
TaaI	ACNGT	2 cut(s)	518, 605
TaiI	ACGT	1 cut(s)	534
TaqI	TCGA	3 cut(s)	332, 552, 636
TasI	AATT	5 cut(s)	84, 226, 437, 674, 681
Tru1I	TTAA	2 cut(s)	327, 677
Tru9I	TTAA	2 cut(s)	327, 677
TscAI	CASTG	2 cut(s)	316, 627
TseFI	GTSAC	1 cut(s)	659
TseI	GCWGC	1 cut(s)	617
Tsp45I	GTSAC	1 cut(s)	659
TspDTI	ATGAA	2 cut(s)	78, 166
TspRI	CASTG	2 cut(s)	316, 627
Van91I	CCANNNNNTGG	1 cut(s)	376
Vha464I	CTTAAG	1 cut(s)	326
VneI	GTGCAC	1 cut(s)	599
XcmI	CCANNNNNNNNNTGG	1 cut(s)	483
XmiI	GTMKAC	1 cut(s)	411

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.