FvH4_1g29360

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0008112
Physical Location & Seq
Reverse (-)
22348391 .. 22350673
2283 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g29360.t1

Sequence Viewer

Length: 747 bp
ATGTATTCTCCAACTCTTGGATCCTATGAGACGGATTCTGCATGCGCTGTTTACGACGTCGCCGACGTCACTGCCTGCACTGCCAAGACCGCTGTCCATTATCACCACAAGATGGAGAATTTTTGTAAGCTAACCTCACCGAGATCCAAAGAGCCTAGCCATGCCTTTTTTCCAGATTCACTTCCTTTTAGGCTTCAAGCAGATGGAAAGATAAAAACTAGTCAAAGCCATTCACCTGCCCAAGTTTTAAGGGATTTCATAACAAGAGTTCACGCCATTCCCACACCCACACCAGTACTATTGTACTCTCTCGTCTCCTCACTCTGCAAAATCAAACTTTTCTCTCACAGACCCAAAAATGGTGCTCGACGTTTCAATCGAGGAGATCGATCAGCCGGAAGTCATCCGCGCCCCGCCAACATCCCCAACCTCTTCACCGTCCATGTGGACGCCAAGCGCATCCTGATCGTCCTCACCGACCACGACGGCGTCGCCTCCAAGTGGCTCACCGAGCTCCTCCGCTCCGAATCCACCAACACCAAGCCTCCTCTCCCCGGCACCGCCTCCACCTTCCCTCCCGACTCCATCTTCGTCGGCCTTGTCACCGAGGAGCACCTCCCCTTTTACGGCCCTCGCCCCTACGACTCCACCAACCACTGCCTCGACCTCCTCACCCTCCGTGTCGGCTCTCATTGTTTCAAAGACGATGACGACGGCGCAGACGAATTGAGGTCAATCCGAGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

249

Amino Acids

27.49

Weight (kDa)

8.5

Isoelectric Point (pI)

57.19

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 244
AatII GACGTC 2 cut(s) 60, 69
Acc36I ACCTGC 1 cut(s) 244
AccB1I GGYRCC 1 cut(s) 557
AccB7I CCANNNNNTGG 2 cut(s) 17, 112
AccBSI CCGCTC 1 cut(s) 522
AccII CGCG 1 cut(s) 409
AciI CCGC 5 cut(s) 90, 407, 414, 520, 561
AclWI GGATC 3 cut(s) 15, 28, 138
AcsI RAATTY 1 cut(s) 118
AcyI GRCGYC 4 cut(s) 57, 66, 450, 489
AfaI GTAC 2 cut(s) 297, 305
AfiI CCNNNNNNNGG 6 cut(s) 17, 112, 359, 501, 554, 626
AgsI TTSAA 3 cut(s) 197, 376, 700
AhlI ACTAGT 1 cut(s) 218
AluBI AGCT 2 cut(s) 130, 514
AluI AGCT 2 cut(s) 130, 514
Alw21I GWGCWC 3 cut(s) 367, 516, 615
Alw26I GTCTC 2 cut(s) 23, 319
AlwI GGATC 3 cut(s) 15, 28, 138
AoxI GGCC 2 cut(s) 595, 628
ApoI RAATTY 1 cut(s) 118
ArsI GACNNNNNNTTYG 2 cut(s) 572, 604
AspLEI GCGC 4 cut(s) 47, 411, 459, 719
AspS9I GGNCC 1 cut(s) 629
AsuC2I CCSGG 1 cut(s) 555
AsuHPI GGTGA 8 cut(s) 95, 129, 225, 427, 466, 499, 595, 664
BamHI GGATCC 1 cut(s) 20
BanI GGYRCC 1 cut(s) 557
BanII GRGCYC 1 cut(s) 516
Bbv12I GWGCWC 3 cut(s) 367, 516, 615
BccI CCATC 3 cut(s) 106, 197, 593
BceAI ACGGC 3 cut(s) 502, 643, 730
BcgI CGANNNNNNTGC 4 cut(s) 53, 87, 448, 482
BcnI CCSGG 1 cut(s) 555
BcoDI GTCTC 2 cut(s) 23, 319
BcuI ACTAGT 1 cut(s) 218
BfaI CTAG 2 cut(s) 156, 219
BfuAI ACCTGC 1 cut(s) 244
BmcAI AGTACT 1 cut(s) 297
Bme1390I CCNGG 1 cut(s) 555
BmgT120I GGNCC 1 cut(s) 629
BmiI GGNNCC 2 cut(s) 22, 559
BmrFI CCNGG 1 cut(s) 555
BmsI GCATC 1 cut(s) 468
BoxI GACNNNNGTC 1 cut(s) 92
BpuMI CCSGG 1 cut(s) 555
Bsa29I ATCGAT 1 cut(s) 388
BsaHI GRCGYC 4 cut(s) 57, 66, 450, 489
BsaJI CCNNGG 3 cut(s) 553, 606, 739
BsaXI ACNNNNNCTCC 4 cut(s) 529, 559, 559, 589
Bsc4I CCNNNNNNNGG 6 cut(s) 17, 112, 359, 501, 554, 626
Bse1I ACTGG 1 cut(s) 293
BseCI ATCGAT 1 cut(s) 388
BseDI CCNNGG 3 cut(s) 553, 606, 739
BseGI GGATG 3 cut(s) 403, 420, 459
BseLI CCNNNNNNNGG 6 cut(s) 17, 112, 359, 501, 554, 626
BseNI ACTGG 1 cut(s) 293
BseRI GAGGAG 6 cut(s) 307, 396, 506, 537, 623, 659
BsgI GTGCAG 1 cut(s) 61
Bsh1236I CGCG 1 cut(s) 409
BshFI GGCC 2 cut(s) 597, 630
BshNI GGYRCC 1 cut(s) 557
BshVI ATCGAT 1 cut(s) 388
BsiHKAI GWGCWC 3 cut(s) 367, 516, 615
BsiSI CCGG 2 cut(s) 396, 555
BslI CCNNNNNNNGG 6 cut(s) 17, 112, 359, 501, 554, 626
BsmAI GTCTC 2 cut(s) 23, 319
BsmBI CGTCTC 2 cut(s) 23, 319
BsnI GGCC 2 cut(s) 597, 630
Bsp1286I GDGCHC 3 cut(s) 367, 516, 615
Bsp143I GATC 5 cut(s) 20, 143, 385, 389, 465
BspACI CCGC 5 cut(s) 90, 407, 414, 520, 561
BspANI GGCC 2 cut(s) 597, 630
BspDI ATCGAT 1 cut(s) 388
BspFNI CGCG 1 cut(s) 409
BspLI GGNNCC 2 cut(s) 22, 559
BspMI ACCTGC 1 cut(s) 244
BspPI GGATC 3 cut(s) 15, 28, 138
BspT107I GGYRCC 1 cut(s) 557
BsrBI CCGCTC 1 cut(s) 522
BsrI ACTGG 1 cut(s) 293
BssECI CCNNGG 3 cut(s) 553, 606, 739
BssMI GATC 5 cut(s) 20, 143, 385, 389, 465
BssNI GRCGYC 4 cut(s) 57, 66, 450, 489
Bst4CI ACNGT 1 cut(s) 439
Bst6I CTCTTC 1 cut(s) 437
BstACI GRCGYC 4 cut(s) 57, 66, 450, 489
BstC8I GCNNGC 2 cut(s) 43, 76
BstF5I GGATG 3 cut(s) 403, 420, 459
BstFNI CGCG 1 cut(s) 409
BstHHI GCGC 4 cut(s) 47, 411, 459, 719
BstKTI GATC 5 cut(s) 23, 146, 388, 392, 468
BstMAI GTCTC 2 cut(s) 23, 319
BstMBI GATC 5 cut(s) 20, 143, 385, 389, 465
BstMWI GCNNNNNNNGC 3 cut(s) 80, 89, 511
BstNSI RCATGY 1 cut(s) 45
BstPAI GACNNNNGTC 1 cut(s) 92
BstSCI CCNGG 1 cut(s) 553
BstUI CGCG 1 cut(s) 409
BstX2I RGATCY 2 cut(s) 20, 143
BstYI RGATCY 2 cut(s) 20, 143
Bsu15I ATCGAT 1 cut(s) 388
BsuRI GGCC 2 cut(s) 597, 630
BsuTUI ATCGAT 1 cut(s) 388
BtsCI GGATG 3 cut(s) 403, 420, 459
BtsI GCAGTG 3 cut(s) 69, 78, 655
BtsIMutI CAGTG 3 cut(s) 69, 78, 655
BveI ACCTGC 1 cut(s) 244
Cac8I GCNNGC 2 cut(s) 43, 76
CfoI GCGC 4 cut(s) 47, 411, 459, 719
Cfr13I GGNCC 1 cut(s) 629
ClaI ATCGAT 1 cut(s) 388
CseI GACGC 2 cut(s) 458, 478
Csp6I GTAC 2 cut(s) 296, 304
CviAII CATG 3 cut(s) 42, 161, 443
CviQI GTAC 2 cut(s) 296, 304
DpnI GATC 5 cut(s) 22, 145, 387, 391, 467
DpnII GATC 5 cut(s) 20, 143, 385, 389, 465
Eam1104I CTCTTC 1 cut(s) 437
EarI CTCTTC 1 cut(s) 437
Ecl136II GAGCTC 1 cut(s) 514
Eco24I GRGCYC 1 cut(s) 516
Eco53kI GAGCTC 1 cut(s) 514
EcoICRI GAGCTC 1 cut(s) 514
EcoT38I GRGCYC 1 cut(s) 516
Esp3I CGTCTC 2 cut(s) 23, 319
FaeI CATG 3 cut(s) 45, 164, 446
FaiI YATR 5 cut(s) 27, 43, 162, 260, 444
FatI CATG 3 cut(s) 41, 160, 442
FauI CCCGC 1 cut(s) 421
FokI GGATG 3 cut(s) 390, 407, 446
FriOI GRGCYC 1 cut(s) 516
FspBI CTAG 2 cut(s) 156, 219
GlaI GCGC 4 cut(s) 46, 410, 458, 718
HaeIII GGCC 2 cut(s) 597, 630
HapII CCGG 2 cut(s) 396, 555
HgaI GACGC 2 cut(s) 458, 478
HhaI GCGC 4 cut(s) 47, 411, 459, 719
Hin1I GRCGYC 4 cut(s) 57, 66, 450, 489
Hin1II CATG 3 cut(s) 45, 164, 446
Hin6I GCGC 4 cut(s) 45, 409, 457, 717
HinP1I GCGC 4 cut(s) 45, 409, 457, 717
HinfI GANTC 5 cut(s) 35, 176, 527, 581, 644
HpaII CCGG 2 cut(s) 396, 555
HphI GGTGA 8 cut(s) 95, 129, 225, 427, 466, 499, 595, 664
Hpy166II GTNNAC 3 cut(s) 52, 271, 448
Hpy188I TCNGA 2 cut(s) 526, 740
Hpy188III TCNNGA 3 cut(s) 173, 463, 578
Hpy8I GTNNAC 3 cut(s) 52, 271, 448
Hpy99I CGWCG 8 cut(s) 59, 62, 68, 372, 488, 494, 596, 716
HpyAV CCTTC 1 cut(s) 580
HpyCH4III ACNGT 1 cut(s) 439
HpyCH4IV ACGT 3 cut(s) 57, 66, 370
HpyCH4V TGCA 3 cut(s) 41, 78, 327
HpyF10VI GCNNNNNNNGC 3 cut(s) 80, 89, 511
HpySE526I ACGT 3 cut(s) 57, 66, 370
Hsp92I GRCGYC 4 cut(s) 57, 66, 450, 489
Hsp92II CATG 3 cut(s) 45, 164, 446
HspAI GCGC 4 cut(s) 45, 409, 457, 717
Kzo9I GATC 5 cut(s) 20, 143, 385, 389, 465
LmnI GCTCC 3 cut(s) 519, 527, 610
LpnPI CCDG 7 cut(s) 88, 186, 249, 306, 409, 476, 568
LweI GCATC 1 cut(s) 468
MaeI CTAG 2 cut(s) 156, 219
MaeII ACGT 3 cut(s) 57, 66, 370
MaeIII GTNAC 2 cut(s) 67, 601
MalI GATC 5 cut(s) 22, 145, 387, 391, 467
MbiI CCGCTC 1 cut(s) 522
MboI GATC 5 cut(s) 20, 143, 385, 389, 465
MboII GAAGA 2 cut(s) 424, 580
MflI RGATCY 2 cut(s) 20, 143
MhlI GDGCHC 3 cut(s) 367, 516, 615
MluCI AATT 2 cut(s) 118, 725
MlyI GAGTC 2 cut(s) 575, 638
MmeI TCCRAC 1 cut(s) 35
MseI TTAA 1 cut(s) 248
MspA1I CMGCKG 1 cut(s) 92
MspI CCGG 2 cut(s) 396, 555
MspR9I CCNGG 1 cut(s) 555
MvnI CGCG 1 cut(s) 409
MwoI GCNNNNNNNGC 3 cut(s) 80, 89, 511
NciI CCSGG 1 cut(s) 555
NdeII GATC 5 cut(s) 20, 143, 385, 389, 465
NlaIII CATG 3 cut(s) 45, 164, 446
NlaIV GGNNCC 2 cut(s) 22, 559
NmuCI GTSAC 2 cut(s) 67, 601
NspI RCATGY 1 cut(s) 45
PaeI GCATGC 1 cut(s) 45
PaqCI CACCTGC 1 cut(s) 244
PcsI WCGNNNNNNNCGW 6 cut(s) 60, 63, 376, 385, 474, 720
PfeI GAWTC 3 cut(s) 35, 176, 527
PflFI GACNNNGTC 1 cut(s) 488
PflMI CCANNNNNTGG 2 cut(s) 17, 112
PleI GAGTC 2 cut(s) 575, 638
PpsI GAGTC 2 cut(s) 575, 638
PshAI GACNNNNGTC 1 cut(s) 92
Psp124BI GAGCTC 1 cut(s) 516
PspN4I GGNNCC 2 cut(s) 22, 559
PspPI GGNCC 1 cut(s) 629
PsuI RGATCY 2 cut(s) 20, 143
PsyI GACNNNGTC 1 cut(s) 488
RsaI GTAC 2 cut(s) 297, 305
RsaNI GTAC 2 cut(s) 296, 304
SacI GAGCTC 1 cut(s) 516
SaqAI TTAA 1 cut(s) 248
Sau3AI GATC 5 cut(s) 20, 143, 385, 389, 465
Sau96I GGNCC 1 cut(s) 629
ScaI AGTACT 1 cut(s) 297
SchI GAGTC 2 cut(s) 575, 638
ScrFI CCNGG 1 cut(s) 555
SduI GDGCHC 3 cut(s) 367, 516, 615
SfaNI GCATC 1 cut(s) 468
SpeI ACTAGT 1 cut(s) 218
SphI GCATGC 1 cut(s) 45
Sse9I AATT 2 cut(s) 118, 725
SsiI CCGC 5 cut(s) 90, 407, 414, 520, 561
SspMI CTAG 2 cut(s) 156, 219
SstI GAGCTC 1 cut(s) 516
StyD4I CCNGG 1 cut(s) 553
TaaI ACNGT 1 cut(s) 439
TaiI ACGT 3 cut(s) 60, 69, 373
TaqI TCGA 4 cut(s) 367, 379, 388, 663
TasI AATT 2 cut(s) 118, 725
TatI WGTACW 2 cut(s) 295, 303
TfiI GAWTC 3 cut(s) 35, 176, 527
Tru1I TTAA 1 cut(s) 248
Tru9I TTAA 1 cut(s) 248
TscAI CASTG 3 cut(s) 76, 85, 662
TseFI GTSAC 2 cut(s) 67, 601
Tsp45I GTSAC 2 cut(s) 67, 601
TspDTI ATGAA 1 cut(s) 247
TspGWI ACGGA 2 cut(s) 47, 668
TspRI CASTG 3 cut(s) 76, 85, 662
Tth111I GACNNNGTC 1 cut(s) 488
Van91I CCANNNNNTGG 2 cut(s) 17, 112
XapI RAATTY 1 cut(s) 118
XceI RCATGY 1 cut(s) 45
XspI CTAG 2 cut(s) 156, 219
ZraI GACGTC 2 cut(s) 58, 67
ZrmI AGTACT 1 cut(s) 297
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.