Prupe.4G184900_v2.0.a1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0008112
Physical Location & Seq
Forward (+)
10965052 .. 10966306
1255 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G184900.1

Sequence Viewer

Length: 480 bp
ATGCTCTGCATCACCATCGGATGCCACTGCCTCATCTACGAGTACTTTGACGACAAACCCGCTGGCTTACTAGTCTCCTTCCTTGCAAACCCTAGGGTTTTCGCCGTGGGCCGCGACATGGCCAATCTGTGCAGGAAGCTCAAGGCGGACCACGGCATCGAGATCCGGAACGCCGTCGACGTCAACGATCTGGCGGCCGTGCTGGGAAAGAAAATGGACGTGGTTAGGCCTGAGGAGGAGCTCTTTTGGCACGGCGAGTATGACTCTTTTTGGAGCATGAATTGTGAGGTGGTCAAGTTCTATACTGTTGATTTGTATCTGTGCTACTTGATCGGTTTGGAGTTGATCGACGCCATTGACGGTGCTGCTGACCTTAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAAAACAAGAAAGACAAAGTAATGCTATTTAGACACACAACATTGATCACCTTAACCGACCACCTTATGTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

160

Amino Acids

18.38

Weight (kDa)

8.66

Isoelectric Point (pI)

25.06

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 183
AccI GTMKAC 1 cut(s) 177
AccII CGCG 1 cut(s) 114
AccIII TCCGGA 1 cut(s) 165
AciI CCGC 4 cut(s) 60, 112, 146, 194
AclWI GGATC 1 cut(s) 157
AcoI YGGCCR 2 cut(s) 120, 195
AcyI GRCGYC 2 cut(s) 180, 351
AfaI GTAC 1 cut(s) 44
AfiI CCNNNNNNNGG 1 cut(s) 118
AflII CTTAAG 1 cut(s) 374
AhlI ACTAGT 1 cut(s) 70
AjiI CACGTC 1 cut(s) 220
AluBI AGCT 2 cut(s) 139, 241
AluI AGCT 2 cut(s) 139, 241
Alw21I GWGCWC 1 cut(s) 243
Alw26I GTCTC 1 cut(s) 79
AlwI GGATC 1 cut(s) 157
Aor13HI TCCGGA 1 cut(s) 165
AoxI GGCC 4 cut(s) 109, 120, 195, 227
ApeKI GCWGC 1 cut(s) 365
AspA2I CCTAGG 1 cut(s) 92
AspS9I GGNCC 2 cut(s) 109, 148
AsuHPI GGTGA 2 cut(s) 4, 445
AvaII GGWCC 1 cut(s) 148
AvrII CCTAGG 1 cut(s) 92
AxyI CCTNAGG 1 cut(s) 231
BalI TGGCCA 1 cut(s) 122
BanII GRGCYC 1 cut(s) 243
Bbv12I GWGCWC 1 cut(s) 243
BbvI GCAGC 1 cut(s) 352
BccI CCATC 1 cut(s) 23
BceAI ACGGC 5 cut(s) 89, 158, 169, 182, 268
BcgI CGANNNNNNTGC 1 cut(s) 32
BclI TGATCA 1 cut(s) 450
BcoDI GTCTC 1 cut(s) 79
BcuI ACTAGT 1 cut(s) 70
BfaI CTAG 2 cut(s) 71, 93
BfrI CTTAAG 1 cut(s) 374
BisI GCNGC 3 cut(s) 112, 195, 366
BlnI CCTAGG 1 cut(s) 92
BlsI GCNGC 3 cut(s) 113, 196, 367
BmcAI AGTACT 1 cut(s) 44
Bme18I GGWCC 1 cut(s) 148
BmgBI CACGTC 1 cut(s) 220
BmgT120I GGNCC 2 cut(s) 109, 148
BmsI GCATC 3 cut(s) 11, 18, 165
BplI GAGNNNNNCTC 2 cut(s) 248, 280
BpuEI CTTGAG 1 cut(s) 125
BsaBI GATNNNNATC 1 cut(s) 315
BsaHI GRCGYC 2 cut(s) 180, 351
BsaJI CCNNGG 3 cut(s) 92, 105, 151
BsaWI WCCGGW 1 cut(s) 165
Bsc4I CCNNNNNNNGG 1 cut(s) 118
Bse21I CCTNAGG 1 cut(s) 231
Bse8I GATNNNNATC 1 cut(s) 315
BseAI TCCGGA 1 cut(s) 165
BseDI CCNNGG 3 cut(s) 92, 105, 151
BseGI GGATG 1 cut(s) 26
BseJI GATNNNNATC 1 cut(s) 315
BseLI CCNNNNNNNGG 1 cut(s) 118
BseMII CTCAG 1 cut(s) 222
BseRI GAGGAG 2 cut(s) 248, 251
BseX3I CGGCCG 1 cut(s) 195
BseXI GCAGC 1 cut(s) 352
BseYI CCCAGC 1 cut(s) 202
BsgI GTGCAG 1 cut(s) 151
Bsh1236I CGCG 1 cut(s) 114
Bsh1285I CGRYCG 1 cut(s) 198
BshFI GGCC 4 cut(s) 111, 122, 197, 229
BsiEI CGRYCG 1 cut(s) 198
BsiHKAI GWGCWC 1 cut(s) 243
BsiSI CCGG 1 cut(s) 166
BslI CCNNNNNNNGG 1 cut(s) 118
BsmAI GTCTC 1 cut(s) 79
BsnI GGCC 4 cut(s) 111, 122, 197, 229
Bsp1286I GDGCHC 1 cut(s) 243
Bsp13I TCCGGA 1 cut(s) 165
Bsp143I GATC 5 cut(s) 162, 187, 330, 345, 450
BspACI CCGC 4 cut(s) 60, 112, 146, 194
BspANI GGCC 4 cut(s) 111, 122, 197, 229
BspCNI CTCAG 1 cut(s) 223
BspEI TCCGGA 1 cut(s) 165
BspFNI CGCG 1 cut(s) 114
BspPI GGATC 1 cut(s) 157
BspTI CTTAAG 1 cut(s) 374
BssECI CCNNGG 3 cut(s) 92, 105, 151
BssMI GATC 5 cut(s) 162, 187, 330, 345, 450
BssNI GRCGYC 2 cut(s) 180, 351
BssT1I CCWWGG 1 cut(s) 92
Bst4CI ACNGT 2 cut(s) 307, 362
BstACI GRCGYC 2 cut(s) 180, 351
BstAFI CTTAAG 1 cut(s) 374
BstC8I GCNNGC 1 cut(s) 64
BstDEI CTNAG 1 cut(s) 231
BstDSI CCRYGG 2 cut(s) 105, 151
BstF5I GGATG 1 cut(s) 26
BstFNI CGCG 1 cut(s) 114
BstKTI GATC 5 cut(s) 165, 190, 333, 348, 453
BstMAI GTCTC 1 cut(s) 79
BstMBI GATC 5 cut(s) 162, 187, 330, 345, 450
BstMCI CGRYCG 1 cut(s) 198
BstMWI GCNNNNNNNGC 1 cut(s) 247
BstUI CGCG 1 cut(s) 114
BstV1I GCAGC 1 cut(s) 352
BstX2I RGATCY 1 cut(s) 162
BstYI RGATCY 1 cut(s) 162
BstZI CGGCCG 1 cut(s) 195
Bsu36I CCTNAGG 1 cut(s) 231
BsuRI GGCC 4 cut(s) 111, 122, 197, 229
BtgI CCRYGG 2 cut(s) 105, 151
BtrI CACGTC 1 cut(s) 220
BtsCI GGATG 1 cut(s) 26
BtsI GCAGTG 1 cut(s) 25
BtsIMutI CAGTG 1 cut(s) 25
Cac8I GCNNGC 1 cut(s) 64
Cfr13I GGNCC 2 cut(s) 109, 148
CseI GACGC 1 cut(s) 359
Csp6I GTAC 1 cut(s) 43
CviAII CATG 2 cut(s) 118, 277
CviJI RGCY 7 cut(s) 66, 111, 122, 139, 197, 229, 241
CviKI_1 RGCY 7 cut(s) 66, 111, 122, 139, 197, 229, 241
CviQI GTAC 1 cut(s) 43
DdeI CTNAG 1 cut(s) 231
DpnI GATC 5 cut(s) 164, 189, 332, 347, 452
DpnII GATC 5 cut(s) 162, 187, 330, 345, 450
EaeI YGGCCR 2 cut(s) 120, 195
EagI CGGCCG 1 cut(s) 195
EciI GGCGGA 1 cut(s) 161
Ecl136II GAGCTC 1 cut(s) 241
EclXI CGGCCG 1 cut(s) 195
Eco130I CCWWGG 1 cut(s) 92
Eco147I AGGCCT 1 cut(s) 229
Eco24I GRGCYC 1 cut(s) 243
Eco47I GGWCC 1 cut(s) 148
Eco52I CGGCCG 1 cut(s) 195
Eco53kI GAGCTC 1 cut(s) 241
Eco81I CCTNAGG 1 cut(s) 231
EcoICRI GAGCTC 1 cut(s) 241
EcoT14I CCWWGG 1 cut(s) 92
EcoT38I GRGCYC 1 cut(s) 243
ErhI CCWWGG 1 cut(s) 92
FaeI CATG 2 cut(s) 121, 280
FaiI YATR 5 cut(s) 119, 261, 278, 303, 473
FatI CATG 2 cut(s) 117, 276
FauI CCCGC 1 cut(s) 67
FbaI TGATCA 1 cut(s) 450
FblI GTMKAC 1 cut(s) 177
Fnu4HI GCNGC 3 cut(s) 112, 195, 366
FokI GGATG 1 cut(s) 33
FriOI GRGCYC 1 cut(s) 243
Fsp4HI GCNGC 3 cut(s) 112, 195, 366
FspBI CTAG 2 cut(s) 71, 93
GluI GCNGC 3 cut(s) 112, 195, 366
GsaI CCCAGC 1 cut(s) 206
HaeIII GGCC 4 cut(s) 111, 122, 197, 229
HapII CCGG 1 cut(s) 166
HgaI GACGC 1 cut(s) 359
Hin1I GRCGYC 2 cut(s) 180, 351
Hin1II CATG 2 cut(s) 121, 280
HincII GTYRAC 2 cut(s) 178, 184
HindII GTYRAC 2 cut(s) 178, 184
HinfI GANTC 1 cut(s) 263
HpaII CCGG 1 cut(s) 166
HphI GGTGA 2 cut(s) 4, 445
Hpy166II GTNNAC 2 cut(s) 178, 184
Hpy188I TCNGA 1 cut(s) 20
Hpy188III TCNNGA 2 cut(s) 160, 166
Hpy8I GTNNAC 2 cut(s) 178, 184
Hpy99I CGWCG 3 cut(s) 179, 182, 353
HpyAV CCTTC 1 cut(s) 88
HpyCH4III ACNGT 2 cut(s) 307, 362
HpyCH4IV ACGT 2 cut(s) 180, 219
HpyCH4V TGCA 3 cut(s) 9, 86, 132
HpyF10VI GCNNNNNNNGC 1 cut(s) 247
HpyF3I CTNAG 1 cut(s) 231
HpySE526I ACGT 2 cut(s) 180, 219
Hsp92I GRCGYC 2 cut(s) 180, 351
Hsp92II CATG 2 cut(s) 121, 280
Kpn2I TCCGGA 1 cut(s) 165
Ksp22I TGATCA 1 cut(s) 450
Kzo9I GATC 5 cut(s) 162, 187, 330, 345, 450
LmnI GCTCC 2 cut(s) 238, 273
LpnPI CCDG 6 cut(s) 48, 118, 176, 179, 188, 243
Lsp1109I GCAGC 1 cut(s) 352
LweI GCATC 3 cut(s) 11, 18, 165
MaeI CTAG 2 cut(s) 71, 93
MaeII ACGT 2 cut(s) 180, 219
MalI GATC 5 cut(s) 164, 189, 332, 347, 452
MboI GATC 5 cut(s) 162, 187, 330, 345, 450
MboII GAAGA 9 cut(s) 391, 394, 397, 400, 403, 406, 409, 412, 415
MflI RGATCY 1 cut(s) 162
MhlI GDGCHC 1 cut(s) 243
MlsI TGGCCA 1 cut(s) 122
MluCI AATT 1 cut(s) 280
MluNI TGGCCA 1 cut(s) 122
MlyI GAGTC 1 cut(s) 257
MnlI CCTC 4 cut(s) 41, 226, 229, 280
Mox20I TGGCCA 1 cut(s) 122
MroI TCCGGA 1 cut(s) 165
MscI TGGCCA 1 cut(s) 122
MseI TTAA 2 cut(s) 375, 458
Msp20I TGGCCA 1 cut(s) 122
MspA1I CMGCKG 1 cut(s) 62
MspCI CTTAAG 1 cut(s) 374
MspI CCGG 1 cut(s) 166
MvnI CGCG 1 cut(s) 114
MwoI GCNNNNNNNGC 1 cut(s) 247
NdeII GATC 5 cut(s) 162, 187, 330, 345, 450
NlaIII CATG 2 cut(s) 121, 280
PceI AGGCCT 1 cut(s) 229
PcsI WCGNNNNNNNCGW 2 cut(s) 177, 183
PkrI GCNGC 3 cut(s) 113, 196, 367
PleI GAGTC 1 cut(s) 257
PpsI GAGTC 1 cut(s) 257
Psp124BI GAGCTC 1 cut(s) 243
PspFI CCCAGC 1 cut(s) 202
PspPI GGNCC 2 cut(s) 109, 148
PsuI RGATCY 1 cut(s) 162
RsaI GTAC 1 cut(s) 44
RsaNI GTAC 1 cut(s) 43
SacI GAGCTC 1 cut(s) 243
SalI GTCGAC 1 cut(s) 176
SaqAI TTAA 2 cut(s) 375, 458
SatI GCNGC 3 cut(s) 112, 195, 366
Sau3AI GATC 5 cut(s) 162, 187, 330, 345, 450
Sau96I GGNCC 2 cut(s) 109, 148
ScaI AGTACT 1 cut(s) 44
SchI GAGTC 1 cut(s) 257
SduI GDGCHC 1 cut(s) 243
SetI ASST 8 cut(s) 141, 183, 222, 243, 291, 375, 458, 471
SfaNI GCATC 3 cut(s) 11, 18, 165
SgrDI CGTCGACG 1 cut(s) 176
SinI GGWCC 1 cut(s) 148
SmlI CTYRAG 2 cut(s) 140, 374
SmoI CTYRAG 2 cut(s) 140, 374
SpeI ACTAGT 1 cut(s) 70
Sse9I AATT 1 cut(s) 280
SseBI AGGCCT 1 cut(s) 229
SsiI CCGC 4 cut(s) 60, 112, 146, 194
SspMI CTAG 2 cut(s) 71, 93
SstI GAGCTC 1 cut(s) 243
StuI AGGCCT 1 cut(s) 229
StyI CCWWGG 1 cut(s) 92
TaaI ACNGT 2 cut(s) 307, 362
TaiI ACGT 2 cut(s) 183, 222
TaqI TCGA 3 cut(s) 159, 177, 348
TasI AATT 1 cut(s) 280
TatI WGTACW 1 cut(s) 42
TauI GCSGC 2 cut(s) 114, 197
Tru1I TTAA 2 cut(s) 375, 458
Tru9I TTAA 2 cut(s) 375, 458
TscAI CASTG 1 cut(s) 32
TseI GCWGC 1 cut(s) 365
TspDTI ATGAA 1 cut(s) 293
TspRI CASTG 1 cut(s) 32
Vha464I CTTAAG 1 cut(s) 374
VpaK11BI GGWCC 1 cut(s) 148
XmaJI CCTAGG 1 cut(s) 92
XmiI GTMKAC 1 cut(s) 177
XspI CTAG 2 cut(s) 71, 93
ZraI GACGTC 1 cut(s) 181
ZrmI AGTACT 1 cut(s) 44
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.