Rh5BG238200

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0008112
Physical Location & Seq
Forward (+)
28404007 .. 28426665
22659 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG238200.1

Sequence Viewer

Length: 963 bp
ATGGTGCTCGAGGTTTCAGTCGAGGAGATCGCAGAGCCGGAGGTCATCCGCGTCTACACCGAGTACCTCCACGACCTCTTCACCGTCCACGTGGACTCCAAGCGCATCCTCACCGTCCTCACCGACAACGAAGCCGTCGCCTCCAAGTGGCTCAAGGAGGTCCTCCGCTCCAAAGCCTCCAACACCAAGCCTCCTCTCCCCGGCTCTCCAACCACCTTCAGCCCCGACTCCATCTTCGTCGGCCTCGTTGCCGAGCAACACTCCCCCTTCTACGGCTCCCGCCCCTACGACGAAACCGACCAGACCTTCGACCTCCTCACCCTCTGCGTCGGCTCTCACTGCCTCATGTACGAGCTCCCTGGCCCCGAGTACCGCCCCTGGTGGTCCGACGAGCTTCCCTACGACGAAGCGCCCAAGGTCTTCAAGGCCTTCTTCCAAAACCCTAGGGTCGTCGTCCTCGGCACCAACATGGCCAAGGTCGCCAAGAGGCTCGAGGCCAGGCACGGCATCCAGATCAAGAACGCCGTGGATCTGAACCAGCTCGCCGTCAAGGGGATGCAGAGGCACGATCTGGACCTGGCTCGCTACGACTTTGAGAAGCTGACCATGACGGTGCTCGGGAAGGCGTTTGACGTCATCAAGCCGGAGAGGGTTTACTGGTATCGGAGGCAGGCGTACTGCAACCGCTGGGGAGTTTTTTACCGCGAAGACTACGATAGGCTGACGAGCGAGAAGGTCATGTTCACCACTGTGGATGCTTATCTGCGCCTCATCATGTGCTTCAAGCTTCTGGAGGCCATGGATGGTCCTGACCCAATTCAATTTACTTCACAAACTGATACAGGATTGGAAAATGGGATGAGGAGATTGGGACATGCCTCTTATTCAAAGAAGAAGTTGTCTCTGTTGTTCATGAAGAAAAAGGACCATCAGAAGCAAACTCTTTTGCTGGCAAATGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

320

Amino Acids

36.95

Weight (kDa)

7.09

Isoelectric Point (pI)

33.87

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 636
AccB1I GGYRCC 1 cut(s) 461
AccBSI CCGCTC 1 cut(s) 168
AccI GTMKAC 1 cut(s) 54
AccII CGCG 2 cut(s) 51, 705
AciI CCGC 6 cut(s) 49, 166, 280, 373, 685, 703
AclWI GGATC 1 cut(s) 537
AcoI YGGCCR 1 cut(s) 471
AcuI CTGAAG 1 cut(s) 202
AcvI CACGTG 1 cut(s) 91
AcyI GRCGYC 1 cut(s) 633
AfaI GTAC 4 cut(s) 65, 350, 371, 677
AfiI CCNNNNNNNGG 4 cut(s) 147, 200, 272, 552
AgsI TTSAA 4 cut(s) 424, 784, 821, 888
AjnI CCWGG 4 cut(s) 358, 377, 497, 576
AleI CACNNNNGTG 1 cut(s) 749
AluBI AGCT 5 cut(s) 355, 394, 541, 601, 787
AluI AGCT 5 cut(s) 355, 394, 541, 601, 787
Alw21I GWGCWC 3 cut(s) 9, 357, 618
Alw26I GTCTC 1 cut(s) 906
AlwI GGATC 1 cut(s) 537
Ama87I CYCGRG 4 cut(s) 8, 365, 491, 617
AoxI GGCC 6 cut(s) 241, 361, 426, 471, 495, 795
ArsI GACNNNNNNTTYG 4 cut(s) 218, 250, 290, 322
AspA2I CCTAGG 1 cut(s) 443
AspLEI GCGC 3 cut(s) 105, 412, 768
AspS9I GGNCC 6 cut(s) 160, 362, 384, 574, 806, 925
AsuC2I CCSGG 1 cut(s) 201
AsuHPI GGTGA 5 cut(s) 73, 103, 112, 310, 736
AvaI CYCGRG 4 cut(s) 8, 365, 491, 617
AvaII GGWCC 5 cut(s) 160, 384, 574, 806, 925
AvrII CCTAGG 1 cut(s) 443
BalI TGGCCA 1 cut(s) 473
BanI GGYRCC 1 cut(s) 461
BanII GRGCYC 1 cut(s) 357
BbrPI CACGTG 1 cut(s) 91
BbsI GAAGAC 2 cut(s) 412, 714
Bbv12I GWGCWC 3 cut(s) 9, 357, 618
BccI CCATC 3 cut(s) 239, 797, 936
BceAI ACGGC 5 cut(s) 119, 289, 509, 520, 530
BciT130I CCWGG 4 cut(s) 360, 379, 499, 578
BcnI CCSGG 1 cut(s) 201
BcoDI GTCTC 1 cut(s) 906
BfaI CTAG 1 cut(s) 444
BfoI RGCGCY 1 cut(s) 413
BlnI CCTAGG 1 cut(s) 443
Bme1390I CCNGG 5 cut(s) 201, 360, 379, 499, 578
Bme18I GGWCC 5 cut(s) 160, 384, 574, 806, 925
BmeT110I CYCGRG 4 cut(s) 8, 365, 491, 617
BmgT120I GGNCC 6 cut(s) 160, 362, 384, 574, 806, 925
BmiI GGNNCC 3 cut(s) 277, 364, 463
BmrFI CCNGG 5 cut(s) 201, 360, 379, 499, 578
BmsI GCATC 4 cut(s) 114, 516, 546, 745
BpiI GAAGAC 2 cut(s) 412, 714
BplI GAGNNNNNCTC 2 cut(s) 245, 277
BpmI CTGGAG 1 cut(s) 812
BpuEI CTTGAG 1 cut(s) 137
BpuMI CCSGG 1 cut(s) 201
BsaAI YACGTR 1 cut(s) 91
BsaBI GATNNNNATC 1 cut(s) 759
BsaHI GRCGYC 1 cut(s) 633
BsaJI CCNNGG 9 cut(s) 199, 358, 377, 414, 443, 457, 474, 525, 798
BsaXI ACNNNNNCTCC 4 cut(s) 80, 110, 175, 205
Bsc4I CCNNNNNNNGG 4 cut(s) 147, 200, 272, 552
Bse1I ACTGG 1 cut(s) 662
Bse8I GATNNNNATC 1 cut(s) 759
BseBI CCWGG 4 cut(s) 360, 379, 499, 578
BseDI CCNNGG 9 cut(s) 199, 358, 377, 414, 443, 457, 474, 525, 798
BseGI GGATG 7 cut(s) 45, 105, 507, 561, 760, 808, 864
BseJI GATNNNNATC 1 cut(s) 759
BseLI CCNNNNNNNGG 4 cut(s) 147, 200, 272, 552
BseNI ACTGG 1 cut(s) 662
BseRI GAGGAG 4 cut(s) 38, 183, 305, 877
BseYI CCCAGC 1 cut(s) 687
Bsh1236I CGCG 2 cut(s) 51, 705
BshFI GGCC 6 cut(s) 243, 363, 428, 473, 497, 797
BshNI GGYRCC 1 cut(s) 461
BsiHKAI GWGCWC 3 cut(s) 9, 357, 618
BsiHKCI CYCGRG 4 cut(s) 8, 365, 491, 617
BsiSI CCGG 3 cut(s) 38, 201, 644
BslFI GGGAC 1 cut(s) 885
BslI CCNNNNNNNGG 4 cut(s) 147, 200, 272, 552
BsmAI GTCTC 1 cut(s) 906
BsmFI GGGAC 1 cut(s) 885
BsnI GGCC 6 cut(s) 243, 363, 428, 473, 497, 797
BsoBI CYCGRG 4 cut(s) 8, 365, 491, 617
Bsp1286I GDGCHC 3 cut(s) 9, 357, 618
Bsp143I GATC 4 cut(s) 27, 513, 529, 568
Bsp19I CCATGG 1 cut(s) 798
BspACI CCGC 6 cut(s) 49, 166, 280, 373, 685, 703
BspANI GGCC 6 cut(s) 243, 363, 428, 473, 497, 797
BspFNI CGCG 2 cut(s) 51, 705
BspHI TCATGA 1 cut(s) 912
BspLI GGNNCC 3 cut(s) 277, 364, 463
BspPI GGATC 1 cut(s) 537
BspT107I GGYRCC 1 cut(s) 461
BsrBI CCGCTC 1 cut(s) 168
BsrI ACTGG 1 cut(s) 662
BssECI CCNNGG 9 cut(s) 199, 358, 377, 414, 443, 457, 474, 525, 798
BssMI GATC 4 cut(s) 27, 513, 529, 568
BssNI GRCGYC 1 cut(s) 633
BssT1I CCWWGG 4 cut(s) 414, 443, 474, 798
Bst2UI CCWGG 4 cut(s) 360, 379, 499, 578
Bst4CI ACNGT 4 cut(s) 85, 115, 613, 751
Bst6I CTCTTC 1 cut(s) 83
BstACI GRCGYC 1 cut(s) 633
BstBAI YACGTR 1 cut(s) 91
BstC8I GCNNGC 4 cut(s) 543, 583, 672, 951
BstDSI CCRYGG 2 cut(s) 525, 798
BstF5I GGATG 7 cut(s) 45, 105, 507, 561, 760, 808, 864
BstFNI CGCG 2 cut(s) 51, 705
BstH2I RGCGCY 1 cut(s) 413
BstHHI GCGC 3 cut(s) 105, 412, 768
BstKTI GATC 4 cut(s) 30, 516, 532, 571
BstMAI GTCTC 1 cut(s) 906
BstMBI GATC 4 cut(s) 27, 513, 529, 568
BstMWI GCNNNNNNNGC 2 cut(s) 339, 479
BstNI CCWGG 4 cut(s) 360, 379, 499, 578
BstNSI RCATGY 1 cut(s) 878
BstSCI CCNGG 5 cut(s) 199, 358, 377, 497, 576
BstUI CGCG 2 cut(s) 51, 705
BstV2I GAAGAC 2 cut(s) 412, 714
BstX2I RGATCY 1 cut(s) 529
BstYI RGATCY 1 cut(s) 529
BsuRI GGCC 6 cut(s) 243, 363, 428, 473, 497, 797
BtgI CCRYGG 2 cut(s) 525, 798
BtsCI GGATG 7 cut(s) 45, 105, 507, 561, 760, 808, 864
BtsI GCAGTG 1 cut(s) 337
BtsIMutI CAGTG 2 cut(s) 337, 747
Cac8I GCNNGC 4 cut(s) 543, 583, 672, 951
CciI TCATGA 1 cut(s) 912
CfoI GCGC 3 cut(s) 105, 412, 768
Cfr13I GGNCC 6 cut(s) 160, 362, 384, 574, 806, 925
CseI GACGC 2 cut(s) 40, 316
Csp6I GTAC 4 cut(s) 64, 349, 370, 676
CviAII CATG 8 cut(s) 346, 469, 607, 739, 775, 799, 875, 913
CviQI GTAC 4 cut(s) 64, 349, 370, 676
DpnI GATC 4 cut(s) 29, 515, 531, 570
DpnII GATC 4 cut(s) 27, 513, 529, 568
EaeI YGGCCR 1 cut(s) 471
Eam1104I CTCTTC 1 cut(s) 83
EarI CTCTTC 1 cut(s) 83
Ecl136II GAGCTC 1 cut(s) 355
Eco130I CCWWGG 4 cut(s) 414, 443, 474, 798
Eco147I AGGCCT 1 cut(s) 428
Eco24I GRGCYC 1 cut(s) 357
Eco47I GGWCC 5 cut(s) 160, 384, 574, 806, 925
Eco53kI GAGCTC 1 cut(s) 355
Eco57I CTGAAG 1 cut(s) 202
Eco72I CACGTG 1 cut(s) 91
Eco88I CYCGRG 4 cut(s) 8, 365, 491, 617
EcoICRI GAGCTC 1 cut(s) 355
EcoO109I RGGNCCY 1 cut(s) 160
EcoRII CCWGG 4 cut(s) 358, 377, 497, 576
EcoT14I CCWWGG 4 cut(s) 414, 443, 474, 798
EcoT22I ATGCAT 1 cut(s) 961
EcoT38I GRGCYC 1 cut(s) 357
ErhI CCWWGG 4 cut(s) 414, 443, 474, 798
FaeI CATG 8 cut(s) 349, 472, 610, 742, 778, 802, 878, 916
FaiI YATR 9 cut(s) 347, 470, 608, 740, 776, 800, 876, 914, 961
FalI AAGNNNNNCTT 2 cut(s) 416, 448
FaqI GGGAC 1 cut(s) 885
FatI CATG 8 cut(s) 345, 468, 606, 738, 774, 798, 874, 912
FauI CCCGC 1 cut(s) 287
FblI GTMKAC 1 cut(s) 54
FokI GGATG 7 cut(s) 32, 92, 494, 568, 767, 815, 871
FriOI GRGCYC 1 cut(s) 357
FspBI CTAG 1 cut(s) 444
GlaI GCGC 3 cut(s) 104, 411, 767
GsaI CCCAGC 1 cut(s) 691
GsuI CTGGAG 1 cut(s) 812
HaeII RGCGCY 1 cut(s) 413
HaeIII GGCC 6 cut(s) 243, 363, 428, 473, 497, 797
HapII CCGG 3 cut(s) 38, 201, 644
HgaI GACGC 2 cut(s) 40, 316
HhaI GCGC 3 cut(s) 105, 412, 768
Hin1I GRCGYC 1 cut(s) 633
Hin1II CATG 8 cut(s) 349, 472, 610, 742, 778, 802, 878, 916
Hin6I GCGC 3 cut(s) 103, 410, 766
HinP1I GCGC 3 cut(s) 103, 410, 766
HindIII AAGCTT 1 cut(s) 785
HinfI GANTC 2 cut(s) 95, 227
HpaII CCGG 3 cut(s) 38, 201, 644
HphI GGTGA 5 cut(s) 73, 103, 112, 310, 736
Hpy166II GTNNAC 5 cut(s) 55, 88, 94, 655, 744
Hpy188I TCNGA 4 cut(s) 388, 534, 666, 933
Hpy188III TCNNGA 7 cut(s) 511, 517, 572, 619, 791, 809, 913
Hpy8I GTNNAC 5 cut(s) 55, 88, 94, 655, 744
Hpy99I CGWCG 7 cut(s) 140, 242, 293, 332, 392, 407, 455
HpyAV CCTTC 6 cut(s) 226, 277, 316, 439, 616, 727
HpyCH4III ACNGT 4 cut(s) 85, 115, 613, 751
HpyCH4IV ACGT 2 cut(s) 90, 633
HpyCH4V TGCA 3 cut(s) 559, 681, 959
HpyF10VI GCNNNNNNNGC 2 cut(s) 339, 479
HpySE526I ACGT 2 cut(s) 90, 633
Hsp92I GRCGYC 1 cut(s) 633
Hsp92II CATG 8 cut(s) 349, 472, 610, 742, 778, 802, 878, 916
HspAI GCGC 3 cut(s) 103, 410, 766
Kzo9I GATC 4 cut(s) 27, 513, 529, 568
LmnI GCTCC 3 cut(s) 173, 281, 360
LweI GCATC 4 cut(s) 114, 516, 546, 745
MaeI CTAG 1 cut(s) 444
MaeII ACGT 2 cut(s) 90, 633
MalI GATC 4 cut(s) 29, 515, 531, 570
MbiI CCGCTC 1 cut(s) 168
MboI GATC 4 cut(s) 27, 513, 529, 568
MboII GAAGA 7 cut(s) 70, 226, 412, 424, 719, 904, 928
MflI RGATCY 1 cut(s) 529
MhlI GDGCHC 3 cut(s) 9, 357, 618
MlsI TGGCCA 1 cut(s) 473
MluCI AATT 2 cut(s) 816, 821
MluNI TGGCCA 1 cut(s) 473
MlyI GAGTC 2 cut(s) 89, 221
MmeI TCCRAC 3 cut(s) 204, 233, 411
Mox20I TGGCCA 1 cut(s) 473
Mph1103I ATGCAT 1 cut(s) 961
MscI TGGCCA 1 cut(s) 473
MslI CAYNNNNRTG 3 cut(s) 467, 611, 749
Msp20I TGGCCA 1 cut(s) 473
MspA1I CMGCKG 1 cut(s) 687
MspI CCGG 3 cut(s) 38, 201, 644
MspR9I CCNGG 5 cut(s) 201, 360, 379, 499, 578
MvaI CCWGG 4 cut(s) 360, 379, 499, 578
MvnI CGCG 2 cut(s) 51, 705
MwoI GCNNNNNNNGC 2 cut(s) 339, 479
NciI CCSGG 1 cut(s) 201
NcoI CCATGG 1 cut(s) 798
NdeII GATC 4 cut(s) 27, 513, 529, 568
NlaIII CATG 8 cut(s) 349, 472, 610, 742, 778, 802, 878, 916
NlaIV GGNNCC 3 cut(s) 277, 364, 463
NmeAIII GCCGAG 2 cut(s) 277, 438
NsiI ATGCAT 1 cut(s) 961
NspI RCATGY 1 cut(s) 878
OliI CACNNNNGTG 1 cut(s) 749
PaeR7I CTCGAG 2 cut(s) 8, 491
PagI TCATGA 1 cut(s) 912
PceI AGGCCT 1 cut(s) 428
PcsI WCGNNNNNNNCGW 2 cut(s) 243, 294
PleI GAGTC 2 cut(s) 89, 221
PmaCI CACGTG 1 cut(s) 91
PmlI CACGTG 1 cut(s) 91
PpsI GAGTC 2 cut(s) 89, 221
Ppu21I YACGTR 1 cut(s) 91
PpuMI RGGWCCY 1 cut(s) 160
Psp124BI GAGCTC 1 cut(s) 357
Psp5II RGGWCCY 1 cut(s) 160
Psp6I CCWGG 4 cut(s) 358, 377, 497, 576
PspCI CACGTG 1 cut(s) 91
PspFI CCCAGC 1 cut(s) 687
PspGI CCWGG 4 cut(s) 358, 377, 497, 576
PspN4I GGNNCC 3 cut(s) 277, 364, 463
PspPI GGNCC 6 cut(s) 160, 362, 384, 574, 806, 925
PspPPI RGGWCCY 1 cut(s) 160
PspXI VCTCGAGB 2 cut(s) 8, 491
PsuI RGATCY 1 cut(s) 529
RsaI GTAC 4 cut(s) 65, 350, 371, 677
RsaNI GTAC 4 cut(s) 64, 349, 370, 676
RseI CAYNNNNRTG 3 cut(s) 467, 611, 749
SacI GAGCTC 1 cut(s) 357
Sau3AI GATC 4 cut(s) 27, 513, 529, 568
Sau96I GGNCC 6 cut(s) 160, 362, 384, 574, 806, 925
SchI GAGTC 2 cut(s) 89, 221
ScrFI CCNGG 5 cut(s) 201, 360, 379, 499, 578
SduI GDGCHC 3 cut(s) 9, 357, 618
SfaNI GCATC 4 cut(s) 114, 516, 546, 745
Sfr274I CTCGAG 2 cut(s) 8, 491
SinI GGWCC 5 cut(s) 160, 384, 574, 806, 925
SlaI CTCGAG 2 cut(s) 8, 491
SmiMI CAYNNNNRTG 3 cut(s) 467, 611, 749
SmlI CTYRAG 3 cut(s) 8, 152, 491
SmoI CTYRAG 3 cut(s) 8, 152, 491
Sse9I AATT 2 cut(s) 816, 821
SseBI AGGCCT 1 cut(s) 428
SsiI CCGC 6 cut(s) 49, 166, 280, 373, 685, 703
SspMI CTAG 1 cut(s) 444
SstI GAGCTC 1 cut(s) 357
StuI AGGCCT 1 cut(s) 428
StyD4I CCNGG 5 cut(s) 199, 358, 377, 497, 576
StyI CCWWGG 4 cut(s) 414, 443, 474, 798
TaaI ACNGT 4 cut(s) 85, 115, 613, 751
TaiI ACGT 2 cut(s) 93, 636
TaqI TCGA 4 cut(s) 9, 21, 309, 492
TasI AATT 2 cut(s) 816, 821
TscAI CASTG 2 cut(s) 344, 754
TspDTI ATGAA 2 cut(s) 901, 929
TspRI CASTG 2 cut(s) 344, 754
VpaK11BI GGWCC 5 cut(s) 160, 384, 574, 806, 925
XceI RCATGY 1 cut(s) 878
XhoI CTCGAG 2 cut(s) 8, 491
XmaJI CCTAGG 1 cut(s) 443
XmiI GTMKAC 1 cut(s) 54
XspI CTAG 1 cut(s) 444
ZraI GACGTC 1 cut(s) 634
Zsp2I ATGCAT 1 cut(s) 961
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.