Prupe.4G185000_v2.0.a1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0008112
Physical Location & Seq
Forward (+)
10971614 .. 10972697
1084 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G185000.1

Sequence Viewer

Length: 732 bp
ATGGTGCTTGAGATCACCGTCGAGCGCGACTACACCATCGAAGACCCCAAAACGGACGCCTACACCGTCCGCGTGGACACCCAGCGCATCCTGACCGTCGTTACCCACTCCGAGTCCATCGTCACCAAATGGCTCAAGGACGCCCTCAAGTCCTCCATCAAATCTGCCGTTCTCGTCGGCATCACGGCCGAGACCGAGAATAGGTGGTGCTGGCACGAAAAGGACAGGCACAAGGAGTATCCGTACGACTTTCTCTGCCTCAACATCGGGGCCCAATCCCTCGTCTACGAGACGTGGGAGACATATTTCAGAGGCAGACACTCGCTCGTCTCCTTCCTCGAAAACCCTAGGGTTTTCGTCATCGGCCGCGAAATGGCCAATATCTCCAGGAAGCTCAAGGTTCACCACGGCATCGAGATCCGGAACGCCGTCGACGTCAACGATCTCGCGATCAGGGGGCTGAGGAGGGACGACCTGGATCTTGGCCGGTACGATCTAGATCAGCTCGCCAGGGCCGTGCTCGGGAAGCAAATGGACTTGGCTAGGCCTGAGGACGATTTCTACTGGAAATATAGGGACGATGAGTTCCATCGTTGGACCCAGTGCGACGAGCTGAAGTTATTCTGGGCCATTGATCCCTATCTGTGCTTCCTGATCGGTTTGGAGTTGATCGACGCCATTGACGGTGCTGCTAGCCAAAAGAAGAAGACGAAGAAGAAGAAGAAGAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

244

Amino Acids

28.57

Weight (kDa)

6.67

Isoelectric Point (pI)

33.66

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 438
AccI GTMKAC 2 cut(s) 285, 432
AccII CGCG 4 cut(s) 27, 72, 369, 449
AccIII TCCGGA 1 cut(s) 420
AciI CCGC 2 cut(s) 70, 367
AclWI GGATC 3 cut(s) 412, 486, 629
AcoI YGGCCR 4 cut(s) 186, 364, 375, 484
AcuI CTGAAG 1 cut(s) 635
AcyI GRCGYC 4 cut(s) 57, 141, 435, 675
AfaI GTAC 2 cut(s) 245, 491
AfiI CCNNNNNNNGG 4 cut(s) 52, 201, 373, 522
AjiI CACGTC 1 cut(s) 294
AjnI CCWGG 3 cut(s) 386, 474, 509
AloI GAACNNNNNNTCC 2 cut(s) 569, 601
AluBI AGCT 3 cut(s) 394, 505, 613
AluI AGCT 3 cut(s) 394, 505, 613
Alw21I GWGCWC 1 cut(s) 522
Alw26I GTCTC 4 cut(s) 185, 284, 293, 334
AlwI GGATC 3 cut(s) 412, 486, 629
Ama87I CYCGRG 1 cut(s) 521
Aor13HI TCCGGA 1 cut(s) 420
AoxI GGCC 8 cut(s) 186, 270, 364, 375, 484, 513, 545, 627
ApaI GGGCCC 1 cut(s) 274
ApeKI GCWGC 1 cut(s) 689
Asp700I GAANNNNTTC 1 cut(s) 620
AspA2I CCTAGG 1 cut(s) 347
AspLEI GCGC 2 cut(s) 27, 87
AspS9I GGNCC 5 cut(s) 270, 271, 513, 597, 627
AsuHPI GGTGA 3 cut(s) 7, 115, 395
AsuNHI GCTAGC 1 cut(s) 692
AvaI CYCGRG 1 cut(s) 521
AvaII GGWCC 1 cut(s) 597
AvrII CCTAGG 1 cut(s) 347
AxyI CCTNAGG 1 cut(s) 549
BaeGI GKGCMC 1 cut(s) 274
BalI TGGCCA 1 cut(s) 377
BanII GRGCYC 1 cut(s) 274
BbsI GAAGAC 2 cut(s) 48, 713
Bbv12I GWGCWC 1 cut(s) 522
BbvCI CCTCAGC 1 cut(s) 461
BbvI GCAGC 1 cut(s) 676
BccI CCATC 4 cut(s) 44, 125, 164, 597
BceAI ACGGC 5 cut(s) 152, 201, 413, 424, 500
BciT130I CCWGG 3 cut(s) 388, 476, 511
BciVI GTATCC 1 cut(s) 249
BcoDI GTCTC 4 cut(s) 185, 284, 293, 334
BfaI CTAG 4 cut(s) 348, 497, 543, 693
BfuI GTATCC 1 cut(s) 249
BisI GCNGC 2 cut(s) 367, 690
BlnI CCTAGG 1 cut(s) 347
BlsI GCNGC 2 cut(s) 368, 691
Bme1390I CCNGG 3 cut(s) 388, 476, 511
Bme18I GGWCC 1 cut(s) 597
BmeT110I CYCGRG 1 cut(s) 521
BmgBI CACGTC 1 cut(s) 294
BmgT120I GGNCC 5 cut(s) 270, 271, 513, 597, 627
BmiI GGNNCC 3 cut(s) 271, 272, 599
BmrFI CCNGG 3 cut(s) 388, 476, 511
BmrI ACTGGG 1 cut(s) 595
BmsI GCATC 3 cut(s) 96, 189, 420
BmtI GCTAGC 1 cut(s) 696
BmuI ACTGGG 1 cut(s) 595
BpiI GAAGAC 2 cut(s) 48, 713
BpmI CTGGAG 1 cut(s) 370
Bpu10I CCTNAGC 1 cut(s) 461
BpuEI CTTGAG 4 cut(s) 29, 119, 131, 380
BsaBI GATNNNNATC 2 cut(s) 498, 639
BsaHI GRCGYC 4 cut(s) 57, 141, 435, 675
BsaI GGTCTC 1 cut(s) 185
BsaJI CCNNGG 3 cut(s) 347, 406, 510
BsaWI WCCGGW 1 cut(s) 420
BsaXI ACNNNNNCTCC 2 cut(s) 227, 257
Bsc4I CCNNNNNNNGG 4 cut(s) 52, 201, 373, 522
Bse118I RCCGGY 1 cut(s) 486
Bse1I ACTGG 2 cut(s) 569, 601
Bse21I CCTNAGG 1 cut(s) 549
Bse8I GATNNNNATC 2 cut(s) 498, 639
BseAI TCCGGA 1 cut(s) 420
BseBI CCWGG 3 cut(s) 388, 476, 511
BseDI CCNNGG 3 cut(s) 347, 406, 510
BseGI GGATG 1 cut(s) 87
BseJI GATNNNNATC 2 cut(s) 498, 639
BseLI CCNNNNNNNGG 4 cut(s) 52, 201, 373, 522
BseMII CTCAG 2 cut(s) 452, 540
BseNI ACTGG 2 cut(s) 569, 601
BseRI GAGGAG 1 cut(s) 478
BseSI GKGCMC 1 cut(s) 274
BseX3I CGGCCG 2 cut(s) 186, 364
BseXI GCAGC 1 cut(s) 676
BseYI CCCAGC 1 cut(s) 81
Bsh1236I CGCG 4 cut(s) 27, 72, 369, 449
Bsh1285I CGRYCG 2 cut(s) 189, 367
BshFI GGCC 8 cut(s) 188, 272, 366, 377, 486, 515, 547, 629
BsiEI CGRYCG 2 cut(s) 189, 367
BsiHKAI GWGCWC 1 cut(s) 522
BsiHKCI CYCGRG 1 cut(s) 521
BsiSI CCGG 2 cut(s) 421, 487
BsiWI CGTACG 1 cut(s) 243
BslFI GGGAC 2 cut(s) 482, 590
BslI CCNNNNNNNGG 4 cut(s) 52, 201, 373, 522
BsmAI GTCTC 4 cut(s) 185, 284, 293, 334
BsmBI CGTCTC 2 cut(s) 284, 334
BsmFI GGGAC 2 cut(s) 482, 590
BsnI GGCC 8 cut(s) 188, 272, 366, 377, 486, 515, 547, 629
Bso31I GGTCTC 1 cut(s) 185
BsoBI CYCGRG 1 cut(s) 521
Bsp120I GGGCCC 1 cut(s) 270
Bsp1286I GDGCHC 2 cut(s) 274, 522
Bsp13I TCCGGA 1 cut(s) 420
Bsp68I TCGCGA 1 cut(s) 449
BspACI CCGC 2 cut(s) 70, 367
BspANI GGCC 8 cut(s) 188, 272, 366, 377, 486, 515, 547, 629
BspCNI CTCAG 2 cut(s) 453, 541
BspEI TCCGGA 1 cut(s) 420
BspFNI CGCG 4 cut(s) 27, 72, 369, 449
BspLI GGNNCC 3 cut(s) 271, 272, 599
BspOI GCTAGC 1 cut(s) 696
BspPI GGATC 3 cut(s) 412, 486, 629
BspTNI GGTCTC 1 cut(s) 185
BsrFI RCCGGY 1 cut(s) 486
BsrI ACTGG 2 cut(s) 569, 601
BssAI RCCGGY 1 cut(s) 486
BssECI CCNNGG 3 cut(s) 347, 406, 510
BssNI GRCGYC 4 cut(s) 57, 141, 435, 675
BssT1I CCWWGG 1 cut(s) 347
Bst2UI CCWGG 3 cut(s) 388, 476, 511
Bst4CI ACNGT 4 cut(s) 19, 67, 97, 686
BstACI GRCGYC 4 cut(s) 57, 141, 435, 675
BstC8I GCNNGC 3 cut(s) 212, 507, 694
BstDEI CTNAG 2 cut(s) 461, 549
BstDSI CCRYGG 1 cut(s) 406
BstF5I GGATG 1 cut(s) 87
BstFNI CGCG 4 cut(s) 27, 72, 369, 449
BstHHI GCGC 2 cut(s) 27, 87
BstMAI GTCTC 4 cut(s) 185, 284, 293, 334
BstMCI CGRYCG 2 cut(s) 189, 367
BstMWI GCNNNNNNNGC 1 cut(s) 526
BstNI CCWGG 3 cut(s) 388, 476, 511
BstSCI CCNGG 3 cut(s) 386, 474, 509
BstSLI GKGCMC 1 cut(s) 274
BstUI CGCG 4 cut(s) 27, 72, 369, 449
BstV1I GCAGC 1 cut(s) 676
BstV2I GAAGAC 2 cut(s) 48, 713
BstX2I RGATCY 2 cut(s) 417, 478
BstYI RGATCY 2 cut(s) 417, 478
BstZI CGGCCG 2 cut(s) 186, 364
Bsu36I CCTNAGG 1 cut(s) 549
BsuI GTATCC 1 cut(s) 249
BsuRI GGCC 8 cut(s) 188, 272, 366, 377, 486, 515, 547, 629
BtgI CCRYGG 1 cut(s) 406
BtrI CACGTC 1 cut(s) 294
BtsCI GGATG 1 cut(s) 87
BtsIMutI CAGTG 1 cut(s) 608
BtuMI TCGCGA 1 cut(s) 449
Cac8I GCNNGC 3 cut(s) 212, 507, 694
CfoI GCGC 2 cut(s) 27, 87
Cfr10I RCCGGY 1 cut(s) 486
Cfr13I GGNCC 5 cut(s) 270, 271, 513, 597, 627
CseI GACGC 3 cut(s) 65, 149, 683
Csp6I GTAC 2 cut(s) 244, 490
CviQI GTAC 2 cut(s) 244, 490
DdeI CTNAG 2 cut(s) 461, 549
EaeI YGGCCR 4 cut(s) 186, 364, 375, 484
EagI CGGCCG 2 cut(s) 186, 364
EclXI CGGCCG 2 cut(s) 186, 364
Eco130I CCWWGG 1 cut(s) 347
Eco147I AGGCCT 1 cut(s) 547
Eco24I GRGCYC 1 cut(s) 274
Eco31I GGTCTC 1 cut(s) 185
Eco47I GGWCC 1 cut(s) 597
Eco52I CGGCCG 2 cut(s) 186, 364
Eco57I CTGAAG 1 cut(s) 635
Eco81I CCTNAGG 1 cut(s) 549
Eco88I CYCGRG 1 cut(s) 521
EcoO109I RGGNCCY 1 cut(s) 270
EcoRII CCWGG 3 cut(s) 386, 474, 509
EcoT14I CCWWGG 1 cut(s) 347
EcoT38I GRGCYC 1 cut(s) 274
ErhI CCWWGG 1 cut(s) 347
Esp3I CGTCTC 2 cut(s) 284, 334
FaiI YATR 2 cut(s) 304, 573
FaqI GGGAC 2 cut(s) 482, 590
FblI GTMKAC 2 cut(s) 285, 432
Fnu4HI GCNGC 2 cut(s) 367, 690
FokI GGATG 1 cut(s) 74
FriOI GRGCYC 1 cut(s) 274
Fsp4HI GCNGC 2 cut(s) 367, 690
FspBI CTAG 4 cut(s) 348, 497, 543, 693
GlaI GCGC 2 cut(s) 26, 86
GluI GCNGC 2 cut(s) 367, 690
GsaI CCCAGC 1 cut(s) 85
GsuI CTGGAG 1 cut(s) 370
HaeIII GGCC 8 cut(s) 188, 272, 366, 377, 486, 515, 547, 629
HapII CCGG 2 cut(s) 421, 487
HgaI GACGC 3 cut(s) 65, 149, 683
HhaI GCGC 2 cut(s) 27, 87
Hin1I GRCGYC 4 cut(s) 57, 141, 435, 675
Hin6I GCGC 2 cut(s) 25, 85
HinP1I GCGC 2 cut(s) 25, 85
HincII GTYRAC 2 cut(s) 433, 439
HindII GTYRAC 2 cut(s) 433, 439
HinfI GANTC 1 cut(s) 113
HpaII CCGG 2 cut(s) 421, 487
HphI GGTGA 3 cut(s) 7, 115, 395
Hpy166II GTNNAC 5 cut(s) 76, 286, 403, 433, 439
Hpy188I TCNGA 2 cut(s) 112, 311
Hpy188III TCNNGA 7 cut(s) 91, 415, 421, 448, 497, 523, 652
Hpy8I GTNNAC 5 cut(s) 76, 286, 403, 433, 439
Hpy99I CGWCG 7 cut(s) 23, 101, 179, 434, 437, 611, 677
HpyAV CCTTC 1 cut(s) 343
HpyCH4III ACNGT 4 cut(s) 19, 67, 97, 686
HpyCH4IV ACGT 2 cut(s) 293, 435
HpyF10VI GCNNNNNNNGC 1 cut(s) 526
HpyF3I CTNAG 2 cut(s) 461, 549
HpySE526I ACGT 2 cut(s) 293, 435
Hsp92I GRCGYC 4 cut(s) 57, 141, 435, 675
HspAI GCGC 2 cut(s) 25, 85
Kpn2I TCCGGA 1 cut(s) 420
Lsp1109I GCAGC 1 cut(s) 676
LweI GCATC 3 cut(s) 96, 189, 420
MaeI CTAG 4 cut(s) 348, 497, 543, 693
MaeII ACGT 2 cut(s) 293, 435
MaeIII GTNAC 2 cut(s) 100, 121
MboII GAAGA 6 cut(s) 53, 715, 718, 724, 727, 730
MflI RGATCY 2 cut(s) 417, 478
MhlI GDGCHC 2 cut(s) 274, 522
MlsI TGGCCA 1 cut(s) 377
MluCI AATT 1 cut(s) 727
MluNI TGGCCA 1 cut(s) 377
MlyI GAGTC 1 cut(s) 122
MmeI TCCRAC 1 cut(s) 575
MnlI CCTC 9 cut(s) 155, 163, 269, 290, 305, 347, 456, 459, 544
Mox20I TGGCCA 1 cut(s) 377
MroI TCCGGA 1 cut(s) 420
MroXI GAANNNNTTC 1 cut(s) 620
MscI TGGCCA 1 cut(s) 377
MseI TTAA 1 cut(s) 730
Msp20I TGGCCA 1 cut(s) 377
MspI CCGG 2 cut(s) 421, 487
MspR9I CCNGG 3 cut(s) 388, 476, 511
MvaI CCWGG 3 cut(s) 388, 476, 511
MvnI CGCG 4 cut(s) 27, 72, 369, 449
MwoI GCNNNNNNNGC 1 cut(s) 526
NheI GCTAGC 1 cut(s) 692
NlaIV GGNNCC 3 cut(s) 271, 272, 599
NmeAIII GCCGAG 1 cut(s) 214
NmuCI GTSAC 1 cut(s) 121
NruI TCGCGA 1 cut(s) 449
PceI AGGCCT 1 cut(s) 547
PcsI WCGNNNNNNNCGW 4 cut(s) 63, 432, 438, 513
PdmI GAANNNNTTC 1 cut(s) 620
Pfl23II CGTACG 1 cut(s) 243
PfoI TCCNGGA 1 cut(s) 386
PkrI GCNGC 2 cut(s) 368, 691
PleI GAGTC 1 cut(s) 121
PpsI GAGTC 1 cut(s) 121
Psp6I CCWGG 3 cut(s) 386, 474, 509
PspFI CCCAGC 1 cut(s) 81
PspGI CCWGG 3 cut(s) 386, 474, 509
PspLI CGTACG 1 cut(s) 243
PspN4I GGNNCC 3 cut(s) 271, 272, 599
PspOMI GGGCCC 1 cut(s) 270
PspPI GGNCC 5 cut(s) 270, 271, 513, 597, 627
PsuI RGATCY 2 cut(s) 417, 478
RruI TCGCGA 1 cut(s) 449
RsaI GTAC 2 cut(s) 245, 491
RsaNI GTAC 2 cut(s) 244, 490
SalI GTCGAC 1 cut(s) 431
SaqAI TTAA 1 cut(s) 730
SatI GCNGC 2 cut(s) 367, 690
Sau96I GGNCC 5 cut(s) 270, 271, 513, 597, 627
SchI GAGTC 1 cut(s) 122
ScrFI CCNGG 3 cut(s) 388, 476, 511
SduI GDGCHC 2 cut(s) 274, 522
SetI ASST 8 cut(s) 206, 296, 396, 402, 438, 477, 507, 615
SfaNI GCATC 3 cut(s) 96, 189, 420
SgrDI CGTCGACG 1 cut(s) 431
SinI GGWCC 1 cut(s) 597
SmlI CTYRAG 4 cut(s) 8, 134, 146, 395
SmoI CTYRAG 4 cut(s) 8, 134, 146, 395
Sse9I AATT 1 cut(s) 727
SseBI AGGCCT 1 cut(s) 547
SsiI CCGC 2 cut(s) 70, 367
SspMI CTAG 4 cut(s) 348, 497, 543, 693
StuI AGGCCT 1 cut(s) 547
StyD4I CCNGG 3 cut(s) 386, 474, 509
StyI CCWWGG 1 cut(s) 347
TaaI ACNGT 4 cut(s) 19, 67, 97, 686
TaiI ACGT 2 cut(s) 296, 438
TaqI TCGA 6 cut(s) 21, 39, 339, 414, 432, 672
TaqII GACCGA 1 cut(s) 209
TasI AATT 1 cut(s) 727
TauI GCSGC 1 cut(s) 369
Tru1I TTAA 1 cut(s) 730
Tru9I TTAA 1 cut(s) 730
TscAI CASTG 1 cut(s) 608
TseFI GTSAC 1 cut(s) 121
TseI GCWGC 1 cut(s) 689
Tsp45I GTSAC 1 cut(s) 121
TspGWI ACGGA 2 cut(s) 68, 231
TspRI CASTG 1 cut(s) 608
VpaK11BI GGWCC 1 cut(s) 597
XbaI TCTAGA 1 cut(s) 496
XmaJI CCTAGG 1 cut(s) 347
XmiI GTMKAC 2 cut(s) 285, 432
XmnI GAANNNNTTC 1 cut(s) 620
XspI CTAG 4 cut(s) 348, 497, 543, 693
ZraI GACGTC 1 cut(s) 436
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.