Rh5AG237500

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0008112
Physical Location & Seq
Forward (+)
28842095 .. 28843545
1451 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG237500.1

Sequence Viewer

Length: 774 bp
ATGGTGCTCGAGGTTTCAGTCGAGGAGATCACAGAGCCGGAGGTCATCCGCGTCTACACCGAGTACCTCCACGACCTCTTCACCATCCATGTGGACTCTAAGCGCATCCTCACTGTCCTCACCGACAACGAAGCCGTCGCCTCCAAGTGGCTCAAGGAGGTCCTCCGCTCCAAAGCCTCCAACACCAAGCCTCCTCTCCCCGGCGCTCCCACCACCTTCATCCCCGACTCCATCTTCGTCGGTCTCGTCGCCGAGCAACACTCCCCCTTCTACGGCTCCCGCCCCTACGACGAAACCGACCAGACCTTCGACCTCCTCACCCTCTGCGTCGGCTCTCACTGCCTCATGTACGAGCTCCCTGGCCCCGAGTACCGCCCCTGGTGGTCCGACGAGCTTCCCTACGACGACGCGCCCAAGGTCTTCAAGGCCTTCTTCCAAAACCCTAGGGTCGTCGTCCTCGGCACCAGCATGGCCAAGGTCGCCAAGAGGCTCGAGGCCAGGCACGGCATCCAGATCAAAAACGCCGTGGATCTGAACCAGCTCGCCGTCAAAGGGATGCAGAGGCACGATCTGGACCTGGCTCGCTACGACTTTGAGAAGCTGACCATGACGGTGCTCGGGAAGGAGTTTGACGTCATCAAGCCGGAGAGGGTTTACTGGTATCGGAGGCAGGCGTACTGCAACCGCTGGGGAATTTTTTACCGCGAAGACTGCGATAGGCTGACGAGCGAGAAGGTCCAGCTGTGGCCTGCAAATTCTCCGTCAAAAAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

257

Amino Acids

29.78

Weight (kDa)

6.11

Isoelectric Point (pI)

41.61

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 636
AccB1I GGYRCC 1 cut(s) 461
AccBSI CCGCTC 1 cut(s) 168
AccI GTMKAC 1 cut(s) 54
AccII CGCG 3 cut(s) 51, 410, 705
AciI CCGC 6 cut(s) 49, 166, 280, 373, 685, 703
AclWI GGATC 1 cut(s) 537
AcoI YGGCCR 1 cut(s) 471
AcsI RAATTY 2 cut(s) 693, 754
AcyI GRCGYC 1 cut(s) 633
AfaI GTAC 4 cut(s) 65, 350, 371, 677
AfiI CCNNNNNNNGG 4 cut(s) 147, 200, 272, 552
AgsI TTSAA 1 cut(s) 424
AjnI CCWGG 4 cut(s) 358, 377, 497, 576
AluBI AGCT 5 cut(s) 355, 394, 541, 601, 742
AluI AGCT 5 cut(s) 355, 394, 541, 601, 742
Alw21I GWGCWC 3 cut(s) 9, 357, 618
Alw26I GTCTC 1 cut(s) 248
AlwI GGATC 1 cut(s) 537
Ama87I CYCGRG 4 cut(s) 8, 365, 491, 617
AoxI GGCC 5 cut(s) 361, 426, 471, 495, 746
ApoI RAATTY 2 cut(s) 693, 754
ArsI GACNNNNNNTTYG 5 cut(s) 218, 250, 290, 322, 746
AspA2I CCTAGG 1 cut(s) 443
AspLEI GCGC 3 cut(s) 105, 206, 412
AspS9I GGNCC 5 cut(s) 160, 362, 384, 574, 736
AsuC2I CCSGG 1 cut(s) 201
AsuHPI GGTGA 3 cut(s) 73, 112, 310
AvaI CYCGRG 4 cut(s) 8, 365, 491, 617
AvaII GGWCC 4 cut(s) 160, 384, 574, 736
AvrII CCTAGG 1 cut(s) 443
BalI TGGCCA 1 cut(s) 473
BanI GGYRCC 1 cut(s) 461
BanII GRGCYC 1 cut(s) 357
BbsI GAAGAC 2 cut(s) 412, 714
Bbv12I GWGCWC 3 cut(s) 9, 357, 618
BccI CCATC 2 cut(s) 92, 239
BceAI ACGGC 5 cut(s) 119, 289, 509, 520, 530
BciT130I CCWGG 4 cut(s) 360, 379, 499, 578
BcnI CCSGG 1 cut(s) 201
BcoDI GTCTC 1 cut(s) 248
BfaI CTAG 1 cut(s) 444
BfoI RGCGCY 1 cut(s) 207
BlnI CCTAGG 1 cut(s) 443
Bme1390I CCNGG 5 cut(s) 201, 360, 379, 499, 578
Bme18I GGWCC 4 cut(s) 160, 384, 574, 736
BmeT110I CYCGRG 4 cut(s) 8, 365, 491, 617
BmgT120I GGNCC 5 cut(s) 160, 362, 384, 574, 736
BmiI GGNNCC 3 cut(s) 277, 364, 463
BmrFI CCNGG 5 cut(s) 201, 360, 379, 499, 578
BmsI GCATC 3 cut(s) 114, 516, 546
BpiI GAAGAC 2 cut(s) 412, 714
BplI GAGNNNNNCTC 2 cut(s) 245, 277
BpuEI CTTGAG 1 cut(s) 137
BpuMI CCSGG 1 cut(s) 201
BsaHI GRCGYC 1 cut(s) 633
BsaI GGTCTC 1 cut(s) 248
BsaJI CCNNGG 8 cut(s) 199, 358, 377, 414, 443, 457, 474, 525
BsaXI ACNNNNNCTCC 2 cut(s) 175, 205
Bsc4I CCNNNNNNNGG 4 cut(s) 147, 200, 272, 552
Bse1I ACTGG 1 cut(s) 662
BseBI CCWGG 4 cut(s) 360, 379, 499, 578
BseDI CCNNGG 8 cut(s) 199, 358, 377, 414, 443, 457, 474, 525
BseGI GGATG 6 cut(s) 45, 84, 105, 219, 507, 561
BseLI CCNNNNNNNGG 4 cut(s) 147, 200, 272, 552
BseNI ACTGG 1 cut(s) 662
BseRI GAGGAG 3 cut(s) 38, 183, 305
BseYI CCCAGC 1 cut(s) 687
Bsh1236I CGCG 3 cut(s) 51, 410, 705
BshFI GGCC 5 cut(s) 363, 428, 473, 497, 748
BshNI GGYRCC 1 cut(s) 461
BsiHKAI GWGCWC 3 cut(s) 9, 357, 618
BsiHKCI CYCGRG 4 cut(s) 8, 365, 491, 617
BsiSI CCGG 3 cut(s) 38, 201, 644
BslI CCNNNNNNNGG 4 cut(s) 147, 200, 272, 552
BsmAI GTCTC 1 cut(s) 248
BsnI GGCC 5 cut(s) 363, 428, 473, 497, 748
Bso31I GGTCTC 1 cut(s) 248
BsoBI CYCGRG 4 cut(s) 8, 365, 491, 617
Bsp1286I GDGCHC 3 cut(s) 9, 357, 618
Bsp143I GATC 4 cut(s) 27, 513, 529, 568
BspACI CCGC 6 cut(s) 49, 166, 280, 373, 685, 703
BspANI GGCC 5 cut(s) 363, 428, 473, 497, 748
BspFNI CGCG 3 cut(s) 51, 410, 705
BspLI GGNNCC 3 cut(s) 277, 364, 463
BspPI GGATC 1 cut(s) 537
BspT107I GGYRCC 1 cut(s) 461
BspTNI GGTCTC 1 cut(s) 248
BsrBI CCGCTC 1 cut(s) 168
BsrI ACTGG 1 cut(s) 662
BssECI CCNNGG 8 cut(s) 199, 358, 377, 414, 443, 457, 474, 525
BssMI GATC 4 cut(s) 27, 513, 529, 568
BssNI GRCGYC 1 cut(s) 633
BssT1I CCWWGG 3 cut(s) 414, 443, 474
Bst2UI CCWGG 4 cut(s) 360, 379, 499, 578
Bst4CI ACNGT 2 cut(s) 115, 613
Bst6I CTCTTC 1 cut(s) 83
BstACI GRCGYC 1 cut(s) 633
BstC8I GCNNGC 4 cut(s) 543, 583, 672, 750
BstDEI CTNAG 1 cut(s) 99
BstDSI CCRYGG 1 cut(s) 525
BstF5I GGATG 6 cut(s) 45, 84, 105, 219, 507, 561
BstFNI CGCG 3 cut(s) 51, 410, 705
BstH2I RGCGCY 1 cut(s) 207
BstHHI GCGC 3 cut(s) 105, 206, 412
BstKTI GATC 4 cut(s) 30, 516, 532, 571
BstMAI GTCTC 1 cut(s) 248
BstMBI GATC 4 cut(s) 27, 513, 529, 568
BstMWI GCNNNNNNNGC 3 cut(s) 339, 479, 711
BstNI CCWGG 4 cut(s) 360, 379, 499, 578
BstSCI CCNGG 5 cut(s) 199, 358, 377, 497, 576
BstUI CGCG 3 cut(s) 51, 410, 705
BstV2I GAAGAC 2 cut(s) 412, 714
BstX2I RGATCY 1 cut(s) 529
BstXI CCANNNNNNTGG 1 cut(s) 91
BstYI RGATCY 1 cut(s) 529
BsuRI GGCC 5 cut(s) 363, 428, 473, 497, 748
BtgI CCRYGG 1 cut(s) 525
BtsCI GGATG 6 cut(s) 45, 84, 105, 219, 507, 561
BtsI GCAGTG 1 cut(s) 337
BtsIMutI CAGTG 2 cut(s) 111, 337
Cac8I GCNNGC 4 cut(s) 543, 583, 672, 750
CfoI GCGC 3 cut(s) 105, 206, 412
Cfr13I GGNCC 5 cut(s) 160, 362, 384, 574, 736
CseI GACGC 3 cut(s) 40, 316, 416
Csp6I GTAC 4 cut(s) 64, 349, 370, 676
CviAII CATG 4 cut(s) 89, 346, 469, 607
CviQI GTAC 4 cut(s) 64, 349, 370, 676
DdeI CTNAG 1 cut(s) 99
DpnI GATC 4 cut(s) 29, 515, 531, 570
DpnII GATC 4 cut(s) 27, 513, 529, 568
EaeI YGGCCR 1 cut(s) 471
Eam1104I CTCTTC 1 cut(s) 83
EarI CTCTTC 1 cut(s) 83
Ecl136II GAGCTC 1 cut(s) 355
Eco130I CCWWGG 3 cut(s) 414, 443, 474
Eco147I AGGCCT 1 cut(s) 428
Eco24I GRGCYC 1 cut(s) 357
Eco31I GGTCTC 1 cut(s) 248
Eco47I GGWCC 4 cut(s) 160, 384, 574, 736
Eco53kI GAGCTC 1 cut(s) 355
Eco88I CYCGRG 4 cut(s) 8, 365, 491, 617
EcoICRI GAGCTC 1 cut(s) 355
EcoO109I RGGNCCY 1 cut(s) 160
EcoRII CCWGG 4 cut(s) 358, 377, 497, 576
EcoT14I CCWWGG 3 cut(s) 414, 443, 474
EcoT38I GRGCYC 1 cut(s) 357
ErhI CCWWGG 3 cut(s) 414, 443, 474
FaeI CATG 4 cut(s) 92, 349, 472, 610
FaiI YATR 4 cut(s) 90, 347, 470, 608
FalI AAGNNNNNCTT 2 cut(s) 416, 448
FatI CATG 4 cut(s) 88, 345, 468, 606
FauI CCCGC 1 cut(s) 287
FblI GTMKAC 1 cut(s) 54
FokI GGATG 6 cut(s) 32, 71, 92, 206, 494, 568
FriOI GRGCYC 1 cut(s) 357
FspBI CTAG 1 cut(s) 444
GlaI GCGC 3 cut(s) 104, 205, 411
GsaI CCCAGC 1 cut(s) 691
HaeII RGCGCY 1 cut(s) 207
HaeIII GGCC 5 cut(s) 363, 428, 473, 497, 748
HapII CCGG 3 cut(s) 38, 201, 644
HgaI GACGC 3 cut(s) 40, 316, 416
HhaI GCGC 3 cut(s) 105, 206, 412
Hin1I GRCGYC 1 cut(s) 633
Hin1II CATG 4 cut(s) 92, 349, 472, 610
Hin6I GCGC 3 cut(s) 103, 204, 410
HinP1I GCGC 3 cut(s) 103, 204, 410
HinfI GANTC 2 cut(s) 95, 227
HpaII CCGG 3 cut(s) 38, 201, 644
HphI GGTGA 3 cut(s) 73, 112, 310
Hpy166II GTNNAC 3 cut(s) 55, 94, 655
Hpy188I TCNGA 3 cut(s) 388, 534, 666
Hpy188III TCNNGA 3 cut(s) 511, 572, 619
Hpy8I GTNNAC 3 cut(s) 55, 94, 655
Hpy99I CGWCG 9 cut(s) 140, 242, 251, 293, 332, 392, 407, 410, 455
HpyAV CCTTC 6 cut(s) 226, 277, 316, 439, 616, 727
HpyCH4III ACNGT 2 cut(s) 115, 613
HpyCH4IV ACGT 1 cut(s) 633
HpyCH4V TGCA 3 cut(s) 559, 681, 752
HpyF10VI GCNNNNNNNGC 3 cut(s) 339, 479, 711
HpyF3I CTNAG 1 cut(s) 99
HpySE526I ACGT 1 cut(s) 633
Hsp92I GRCGYC 1 cut(s) 633
Hsp92II CATG 4 cut(s) 92, 349, 472, 610
HspAI GCGC 3 cut(s) 103, 204, 410
Kzo9I GATC 4 cut(s) 27, 513, 529, 568
LmnI GCTCC 4 cut(s) 173, 211, 281, 360
LweI GCATC 3 cut(s) 114, 516, 546
MaeI CTAG 1 cut(s) 444
MaeII ACGT 1 cut(s) 633
MalI GATC 4 cut(s) 29, 515, 531, 570
MbiI CCGCTC 1 cut(s) 168
MboI GATC 4 cut(s) 27, 513, 529, 568
MboII GAAGA 5 cut(s) 70, 226, 412, 424, 719
MflI RGATCY 1 cut(s) 529
MhlI GDGCHC 3 cut(s) 9, 357, 618
MlsI TGGCCA 1 cut(s) 473
MluCI AATT 2 cut(s) 693, 754
MluNI TGGCCA 1 cut(s) 473
MlyI GAGTC 2 cut(s) 89, 221
MmeI TCCRAC 2 cut(s) 204, 411
Mox20I TGGCCA 1 cut(s) 473
MscI TGGCCA 1 cut(s) 473
MslI CAYNNNNRTG 3 cut(s) 89, 467, 611
Msp20I TGGCCA 1 cut(s) 473
MspA1I CMGCKG 2 cut(s) 687, 742
MspI CCGG 3 cut(s) 38, 201, 644
MspR9I CCNGG 5 cut(s) 201, 360, 379, 499, 578
MvaI CCWGG 4 cut(s) 360, 379, 499, 578
MvnI CGCG 3 cut(s) 51, 410, 705
MwoI GCNNNNNNNGC 3 cut(s) 339, 479, 711
NciI CCSGG 1 cut(s) 201
NdeII GATC 4 cut(s) 27, 513, 529, 568
NlaIII CATG 4 cut(s) 92, 349, 472, 610
NlaIV GGNNCC 3 cut(s) 277, 364, 463
NmeAIII GCCGAG 2 cut(s) 277, 438
PaeR7I CTCGAG 2 cut(s) 8, 491
PceI AGGCCT 1 cut(s) 428
PcsI WCGNNNNNNNCGW 2 cut(s) 243, 294
PleI GAGTC 2 cut(s) 89, 221
PpsI GAGTC 2 cut(s) 89, 221
PpuMI RGGWCCY 1 cut(s) 160
Psp124BI GAGCTC 1 cut(s) 357
Psp5II RGGWCCY 1 cut(s) 160
Psp6I CCWGG 4 cut(s) 358, 377, 497, 576
PspFI CCCAGC 1 cut(s) 687
PspGI CCWGG 4 cut(s) 358, 377, 497, 576
PspN4I GGNNCC 3 cut(s) 277, 364, 463
PspPI GGNCC 5 cut(s) 160, 362, 384, 574, 736
PspPPI RGGWCCY 1 cut(s) 160
PspXI VCTCGAGB 2 cut(s) 8, 491
PsuI RGATCY 1 cut(s) 529
PvuII CAGCTG 1 cut(s) 742
RsaI GTAC 4 cut(s) 65, 350, 371, 677
RsaNI GTAC 4 cut(s) 64, 349, 370, 676
RseI CAYNNNNRTG 3 cut(s) 89, 467, 611
SacI GAGCTC 1 cut(s) 357
Sau3AI GATC 4 cut(s) 27, 513, 529, 568
Sau96I GGNCC 5 cut(s) 160, 362, 384, 574, 736
SchI GAGTC 2 cut(s) 89, 221
ScrFI CCNGG 5 cut(s) 201, 360, 379, 499, 578
SduI GDGCHC 3 cut(s) 9, 357, 618
SfaNI GCATC 3 cut(s) 114, 516, 546
Sfr274I CTCGAG 2 cut(s) 8, 491
SinI GGWCC 4 cut(s) 160, 384, 574, 736
SlaI CTCGAG 2 cut(s) 8, 491
SmiMI CAYNNNNRTG 3 cut(s) 89, 467, 611
SmlI CTYRAG 3 cut(s) 8, 152, 491
SmoI CTYRAG 3 cut(s) 8, 152, 491
Sse9I AATT 2 cut(s) 693, 754
SseBI AGGCCT 1 cut(s) 428
SsiI CCGC 6 cut(s) 49, 166, 280, 373, 685, 703
SspMI CTAG 1 cut(s) 444
SstI GAGCTC 1 cut(s) 357
StuI AGGCCT 1 cut(s) 428
StyD4I CCNGG 5 cut(s) 199, 358, 377, 497, 576
StyI CCWWGG 3 cut(s) 414, 443, 474
TaaI ACNGT 2 cut(s) 115, 613
TaiI ACGT 1 cut(s) 636
TaqI TCGA 4 cut(s) 9, 21, 309, 492
TaqII GACCGA 1 cut(s) 230
TasI AATT 2 cut(s) 693, 754
TscAI CASTG 2 cut(s) 118, 344
TspDTI ATGAA 1 cut(s) 208
TspGWI ACGGA 1 cut(s) 750
TspRI CASTG 2 cut(s) 118, 344
VpaK11BI GGWCC 4 cut(s) 160, 384, 574, 736
XapI RAATTY 2 cut(s) 693, 754
XhoI CTCGAG 2 cut(s) 8, 491
XmaJI CCTAGG 1 cut(s) 443
XmiI GTMKAC 1 cut(s) 54
XspI CTAG 1 cut(s) 444
ZraI GACGTC 1 cut(s) 634
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.