MD04G1000100.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0008112
Physical Location & Seq
Forward (+)
12735 .. 13135
401 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1000100.v1.1.491

Sequence Viewer

Length: 336 bp
ATGGCCACCGTCTCGTGGAGGCTCAAAGCTCATAACCAGATTGAGATCAGGAACACCGTCGACCTCAATGAGCTAGCGATCAATGGGATGAAAAGAGACAACCTGAATCTCGACCGCTATGATCTCGGACGGCTGATGAATGTTATACTTGGGAAAGAGATGGACGTGGTTTGGCCTAAGAATAAGGTCGAGTGGTTTGGCTATCAATATCGGTGGGAACTCGGAGCTGAGAATGTCAAGTTCTCCACAGTCAACTCGTATATGTGCTTCTTGATCGCTTCGGAGTTGATTGACGTCATTGATTTCACTGCTGCTGGGTTTTGGATGGAGAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

112

Amino Acids

13.04

Weight (kDa)

5.08

Isoelectric Point (pI)

27.29

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 297
AccI GTMKAC 1 cut(s) 60
AciI CCGC 1 cut(s) 115
AcoI YGGCCR 1 cut(s) 3
AcyI GRCGYC 1 cut(s) 294
AfiI CCNNNNNNNGG 1 cut(s) 15
AjiI CACGTC 1 cut(s) 166
AluBI AGCT 3 cut(s) 29, 73, 227
AluI AGCT 3 cut(s) 29, 73, 227
Alw26I GTCTC 2 cut(s) 16, 90
AoxI GGCC 2 cut(s) 3, 173
ApeKI GCWGC 1 cut(s) 311
AsuNHI GCTAGC 1 cut(s) 73
BalI TGGCCA 1 cut(s) 5
BarI GAAGNNNNNNTAC 2 cut(s) 251, 283
BauI CACGAG 1 cut(s) 13
BbvI GCAGC 1 cut(s) 298
BccI CCATC 2 cut(s) 154, 319
BceAI ACGGC 1 cut(s) 146
BcoDI GTCTC 2 cut(s) 16, 90
BfaI CTAG 1 cut(s) 74
BisI GCNGC 1 cut(s) 312
BlsI GCNGC 1 cut(s) 313
BmgBI CACGTC 1 cut(s) 166
BmtI GCTAGC 1 cut(s) 77
BsaBI GATNNNNATC 1 cut(s) 44
BsaHI GRCGYC 1 cut(s) 294
Bsc4I CCNNNNNNNGG 1 cut(s) 15
Bse8I GATNNNNATC 1 cut(s) 44
BseGI GGATG 2 cut(s) 93, 330
BseJI GATNNNNATC 1 cut(s) 44
BseLI CCNNNNNNNGG 1 cut(s) 15
BseMII CTCAG 1 cut(s) 219
BseXI GCAGC 1 cut(s) 298
BseYI CCCAGC 1 cut(s) 314
Bsh1285I CGRYCG 1 cut(s) 115
BshFI GGCC 2 cut(s) 5, 175
BsiEI CGRYCG 1 cut(s) 115
BslI CCNNNNNNNGG 1 cut(s) 15
BsmAI GTCTC 2 cut(s) 16, 90
BsmBI CGTCTC 1 cut(s) 16
BsnI GGCC 2 cut(s) 5, 175
Bsp143I GATC 4 cut(s) 45, 78, 121, 273
BspACI CCGC 1 cut(s) 115
BspANI GGCC 2 cut(s) 5, 175
BspCNI CTCAG 1 cut(s) 220
BspOI GCTAGC 1 cut(s) 77
BssMI GATC 4 cut(s) 45, 78, 121, 273
BssNI GRCGYC 1 cut(s) 294
BssSI CACGAG 1 cut(s) 13
Bst2BI CACGAG 1 cut(s) 13
Bst4CI ACNGT 3 cut(s) 10, 58, 250
BstACI GRCGYC 1 cut(s) 294
BstC8I GCNNGC 1 cut(s) 75
BstDEI CTNAG 2 cut(s) 177, 228
BstF5I GGATG 2 cut(s) 93, 330
BstKTI GATC 4 cut(s) 48, 81, 124, 276
BstMAI GTCTC 2 cut(s) 16, 90
BstMBI GATC 4 cut(s) 45, 78, 121, 273
BstMCI CGRYCG 1 cut(s) 115
BstV1I GCAGC 1 cut(s) 298
BsuRI GGCC 2 cut(s) 5, 175
BtrI CACGTC 1 cut(s) 166
BtsCI GGATG 2 cut(s) 93, 330
BtsI GCAGTG 1 cut(s) 306
BtsIMutI CAGTG 1 cut(s) 306
Cac8I GCNNGC 1 cut(s) 75
CspCI CAANNNNNGTGG 2 cut(s) 194, 229
CviJI RGCY 8 cut(s) 5, 22, 29, 73, 133, 175, 201, 227
CviKI_1 RGCY 8 cut(s) 5, 22, 29, 73, 133, 175, 201, 227
DdeI CTNAG 2 cut(s) 177, 228
DpnI GATC 4 cut(s) 47, 80, 123, 275
DpnII GATC 4 cut(s) 45, 78, 121, 273
EaeI YGGCCR 1 cut(s) 3
Esp3I CGTCTC 1 cut(s) 16
FaiI YATR 5 cut(s) 33, 120, 146, 261, 263
FblI GTMKAC 1 cut(s) 60
Fnu4HI GCNGC 1 cut(s) 312
FokI GGATG 1 cut(s) 100
Fsp4HI GCNGC 1 cut(s) 312
FspBI CTAG 1 cut(s) 74
GluI GCNGC 1 cut(s) 312
GsaI CCCAGC 1 cut(s) 318
HaeIII GGCC 2 cut(s) 5, 175
Hin1I GRCGYC 1 cut(s) 294
HincII GTYRAC 2 cut(s) 61, 253
HindII GTYRAC 2 cut(s) 61, 253
HinfI GANTC 1 cut(s) 106
Hpy166II GTNNAC 2 cut(s) 61, 253
Hpy188I TCNGA 3 cut(s) 128, 224, 283
Hpy188III TCNNGA 3 cut(s) 49, 110, 271
Hpy8I GTNNAC 2 cut(s) 61, 253
Hpy99I CGWCG 1 cut(s) 62
HpyCH4III ACNGT 3 cut(s) 10, 58, 250
HpyCH4IV ACGT 2 cut(s) 165, 294
HpyF3I CTNAG 2 cut(s) 177, 228
HpySE526I ACGT 2 cut(s) 165, 294
Hsp92I GRCGYC 1 cut(s) 294
Kzo9I GATC 4 cut(s) 45, 78, 121, 273
LmnI GCTCC 1 cut(s) 224
LpnPI CCDG 4 cut(s) 34, 50, 116, 300
Lsp1109I GCAGC 1 cut(s) 298
MaeI CTAG 1 cut(s) 74
MaeII ACGT 2 cut(s) 165, 294
MalI GATC 4 cut(s) 47, 80, 123, 275
MboI GATC 4 cut(s) 45, 78, 121, 273
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 1 cut(s) 331
MluNI TGGCCA 1 cut(s) 5
MnlI CCTC 2 cut(s) 12, 74
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 1 cut(s) 334
Msp20I TGGCCA 1 cut(s) 5
NdeII GATC 4 cut(s) 45, 78, 121, 273
NheI GCTAGC 1 cut(s) 73
PfeI GAWTC 1 cut(s) 106
PkrI GCNGC 1 cut(s) 313
PspFI CCCAGC 1 cut(s) 314
SalI GTCGAC 1 cut(s) 59
SaqAI TTAA 1 cut(s) 334
SatI GCNGC 1 cut(s) 312
Sau3AI GATC 4 cut(s) 45, 78, 121, 273
SetI ASST 8 cut(s) 31, 66, 75, 105, 168, 189, 229, 297
Sse9I AATT 1 cut(s) 331
SsiI CCGC 1 cut(s) 115
SspMI CTAG 1 cut(s) 74
TaaI ACNGT 3 cut(s) 10, 58, 250
TaiI ACGT 2 cut(s) 168, 297
TaqI TCGA 3 cut(s) 60, 111, 189
TasI AATT 1 cut(s) 331
TfiI GAWTC 1 cut(s) 106
Tru1I TTAA 1 cut(s) 334
Tru9I TTAA 1 cut(s) 334
TscAI CASTG 1 cut(s) 313
TseI GCWGC 1 cut(s) 311
TspDTI ATGAA 2 cut(s) 104, 152
TspRI CASTG 1 cut(s) 313
XmiI GTMKAC 1 cut(s) 60
XspI CTAG 1 cut(s) 74
ZraI GACGTC 1 cut(s) 295
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.