Rh5BG238100

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0008112
Physical Location & Seq
Forward (+)
28400823 .. 28402907
2085 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG238100.1

Sequence Viewer

Length: 846 bp
ATGGTGCTGGAAATCGCCGTGAAGTTCATGAAGCCTGAGCCGCGAACCGCCGTCGAACTCGACGTAGTCCCAAAGCTCTACCACAGACGCGCCGCCGTCCACGACCTCTACACAGTCCACGTGGACTCCACGCAGATCCTCACCGTCGCCACCCGTGACGACAACATCGCCGGAAAATGGCTCACCGATGTCCTCAAGTCCACCTCCTCCAACACCAAGCCGCTGAACCCTAACCCCGAGTTCAGGCCCGACTCCATCTTCGTCGGCCTAATCGCCGACATGGATTATGAAGTGTACGGCCCCGGCGGCCTCCAGGACCACCCCTACGAGCTCCTCACCTTCTGCGTCGGGTCCCACTGCCTCATGTACCACCTCCCGTGGCCGGACTACCCGGGGACGCCGAAGGTCGTCAAGGCCTTCTTTGAGAACCCCAGGGTGGTGGTGCTCGGAAGGGACATGGCTAAGGTGGCGAACAAGCTGAAGAAAACGCACGGGATCGAGATCAGGAACGCCATCGATCTGAACCAGCTCGCGATCCGAGGGATGAAGAGGGACGATCTGGACCTCGGCCGCTACGATCTGGACCGCCTCTCCATGACCGTGCTGGGGAAGCACTTCGACGTCATCAGGCCGGAGGAGAAGCTCTACGAGTGGTGGACCTACGAAGAAGGCCTCTACGGCCAAGACTCGTACGACGAGCGGCTCTGCGACGAGAAGGTCAAGTTCAGTACTCTTGACACCTATCTCGGCTTGTTGATCGGCACCGCGCTTGCGGAGGCCATTCACGGTGCTCGGTTCTCCGGCGCCACCAAGAAGAAGAAGAAGGCCACCAAAAGGAGCCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

281

Amino Acids

31.97

Weight (kDa)

7.12

Isoelectric Point (pI)

28.35

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 716
AatII GACGTC 1 cut(s) 624
AccB1I GGYRCC 2 cut(s) 761, 803
AccBSI CCGCTC 1 cut(s) 700
AccII CGCG 4 cut(s) 43, 90, 533, 767
AclWI GGATC 3 cut(s) 130, 503, 529
AcoI YGGCCR 3 cut(s) 380, 568, 679
AcuI CTGAAG 1 cut(s) 500
AcvI CACGTG 1 cut(s) 121
AcyI GRCGYC 3 cut(s) 398, 621, 804
AfaI GTAC 4 cut(s) 296, 368, 692, 730
AfiI CCNNNNNNNGG 6 cut(s) 177, 243, 382, 436, 606, 834
AjnI CCWGG 2 cut(s) 312, 431
AluBI AGCT 5 cut(s) 76, 331, 478, 529, 643
AluI AGCT 5 cut(s) 76, 331, 478, 529, 643
Alw21I GWGCWC 3 cut(s) 333, 447, 793
AlwI GGATC 3 cut(s) 130, 503, 529
Ama87I CYCGRG 2 cut(s) 236, 391
ArsI GACNNNNNNTTYG 2 cut(s) 242, 274
Asp700I GAANNNNTTC 1 cut(s) 614
AspLEI GCGC 3 cut(s) 92, 769, 806
AspS9I GGNCC 7 cut(s) 246, 299, 316, 351, 562, 583, 657
AsuC2I CCSGG 3 cut(s) 303, 392, 393
AsuHPI GGTGA 3 cut(s) 133, 175, 328
AvaI CYCGRG 2 cut(s) 236, 391
AvaII GGWCC 5 cut(s) 316, 351, 562, 583, 657
BanI GGYRCC 2 cut(s) 761, 803
BanII GRGCYC 1 cut(s) 333
BbrPI CACGTG 1 cut(s) 121
Bbv12I GWGCWC 3 cut(s) 333, 447, 793
BccI CCATC 2 cut(s) 263, 521
BceAI ACGGC 5 cut(s) 2, 35, 80, 313, 694
BciT130I CCWGG 2 cut(s) 314, 433
BcnI CCSGG 3 cut(s) 303, 392, 393
BfoI RGCGCY 1 cut(s) 807
BglI GCCNNNNNGGC 2 cut(s) 306, 678
BisI GCNGC 6 cut(s) 41, 93, 221, 307, 571, 701
BlsI GCNGC 6 cut(s) 42, 94, 222, 308, 572, 702
BmcAI AGTACT 1 cut(s) 730
Bme1390I CCNGG 5 cut(s) 303, 314, 392, 393, 433
Bme18I GGWCC 5 cut(s) 316, 351, 562, 583, 657
BmeT110I CYCGRG 2 cut(s) 236, 391
BmgT120I GGNCC 7 cut(s) 246, 299, 316, 351, 562, 583, 657
BmiI GGNNCC 6 cut(s) 301, 352, 353, 763, 805, 839
BmrFI CCNGG 5 cut(s) 303, 314, 392, 393, 433
BpmI CTGGAG 1 cut(s) 296
Bpu10I CCTNAGC 2 cut(s) 36, 462
BpuEI CTTGAG 1 cut(s) 179
BpuMI CCSGG 3 cut(s) 303, 392, 393
Bsa29I ATCGAT 1 cut(s) 516
BsaAI YACGTR 1 cut(s) 121
BsaBI GATNNNNATC 1 cut(s) 500
BsaHI GRCGYC 3 cut(s) 398, 621, 804
BsaJI CCNNGG 8 cut(s) 301, 377, 391, 392, 431, 432, 538, 565
BsaXI ACNNNNNCTCC 4 cut(s) 110, 140, 575, 605
Bsc4I CCNNNNNNNGG 6 cut(s) 177, 243, 382, 436, 606, 834
Bse1I ACTGG 1 cut(s) 841
Bse8I GATNNNNATC 1 cut(s) 500
BseBI CCWGG 2 cut(s) 314, 433
BseCI ATCGAT 1 cut(s) 516
BseDI CCNNGG 8 cut(s) 301, 377, 391, 392, 431, 432, 538, 565
BseGI GGATG 1 cut(s) 549
BseJI GATNNNNATC 1 cut(s) 500
BseLI CCNNNNNNNGG 6 cut(s) 177, 243, 382, 436, 606, 834
BseMII CTCAG 1 cut(s) 27
BseNI ACTGG 1 cut(s) 841
BseRI GAGGAG 3 cut(s) 196, 323, 650
BseX3I CGGCCG 1 cut(s) 568
BseYI CCCAGC 1 cut(s) 604
Bsh1236I CGCG 4 cut(s) 43, 90, 533, 767
Bsh1285I CGRYCG 1 cut(s) 571
BshNI GGYRCC 2 cut(s) 761, 803
BshVI ATCGAT 1 cut(s) 516
BsiEI CGRYCG 1 cut(s) 571
BsiHKAI GWGCWC 3 cut(s) 333, 447, 793
BsiHKCI CYCGRG 2 cut(s) 236, 391
BsiSI CCGG 6 cut(s) 171, 303, 383, 392, 632, 801
BsiWI CGTACG 1 cut(s) 690
BslFI GGGAC 5 cut(s) 53, 337, 409, 467, 566
BslI CCNNNNNNNGG 6 cut(s) 177, 243, 382, 436, 606, 834
BsmFI GGGAC 5 cut(s) 53, 337, 409, 467, 566
BsoBI CYCGRG 2 cut(s) 236, 391
Bsp1286I GDGCHC 3 cut(s) 333, 447, 793
Bsp143I GATC 8 cut(s) 135, 495, 501, 517, 534, 556, 577, 756
Bsp68I TCGCGA 1 cut(s) 533
BspCNI CTCAG 1 cut(s) 28
BspDI ATCGAT 1 cut(s) 516
BspFNI CGCG 4 cut(s) 43, 90, 533, 767
BspHI TCATGA 1 cut(s) 27
BspLI GGNNCC 6 cut(s) 301, 352, 353, 763, 805, 839
BspPI GGATC 3 cut(s) 130, 503, 529
BspT107I GGYRCC 2 cut(s) 761, 803
BsrBI CCGCTC 1 cut(s) 700
BsrI ACTGG 1 cut(s) 841
BssECI CCNNGG 8 cut(s) 301, 377, 391, 392, 431, 432, 538, 565
BssMI GATC 8 cut(s) 135, 495, 501, 517, 534, 556, 577, 756
BssNI GRCGYC 3 cut(s) 398, 621, 804
Bst2UI CCWGG 2 cut(s) 314, 433
Bst4CI ACNGT 4 cut(s) 115, 145, 601, 788
Bst6I CTCTTC 1 cut(s) 542
BstACI GRCGYC 3 cut(s) 398, 621, 804
BstBAI YACGTR 1 cut(s) 121
BstC8I GCNNGC 2 cut(s) 531, 771
BstDEI CTNAG 2 cut(s) 36, 462
BstDSI CCRYGG 1 cut(s) 377
BstF5I GGATG 1 cut(s) 549
BstFNI CGCG 4 cut(s) 43, 90, 533, 767
BstH2I RGCGCY 1 cut(s) 807
BstHHI GCGC 3 cut(s) 92, 769, 806
BstKTI GATC 8 cut(s) 138, 498, 504, 520, 537, 559, 580, 759
BstMBI GATC 8 cut(s) 135, 495, 501, 517, 534, 556, 577, 756
BstMCI CGRYCG 1 cut(s) 571
BstMWI GCNNNNNNNGC 5 cut(s) 40, 306, 467, 610, 678
BstNI CCWGG 2 cut(s) 314, 433
BstSCI CCNGG 5 cut(s) 301, 312, 390, 391, 431
BstUI CGCG 4 cut(s) 43, 90, 533, 767
BstX2I RGATCY 1 cut(s) 135
BstXI CCANNNNNNTGG 1 cut(s) 439
BstYI RGATCY 1 cut(s) 135
BstZI CGGCCG 1 cut(s) 568
Bsu15I ATCGAT 1 cut(s) 516
BsuTUI ATCGAT 1 cut(s) 516
BtgI CCRYGG 1 cut(s) 377
BtgZI GCGATG 1 cut(s) 151
BtsCI GGATG 1 cut(s) 549
BtsI GCAGTG 1 cut(s) 355
BtsIMutI CAGTG 1 cut(s) 355
BtuMI TCGCGA 1 cut(s) 533
Cac8I GCNNGC 2 cut(s) 531, 771
CciI TCATGA 1 cut(s) 27
CfoI GCGC 3 cut(s) 92, 769, 806
Cfr13I GGNCC 7 cut(s) 246, 299, 316, 351, 562, 583, 657
Cfr9I CCCGGG 1 cut(s) 391
ClaI ATCGAT 1 cut(s) 516
CseI GACGC 3 cut(s) 96, 334, 406
Csp6I GTAC 4 cut(s) 295, 367, 691, 729
CviAII CATG 5 cut(s) 28, 280, 364, 457, 595
CviQI GTAC 4 cut(s) 295, 367, 691, 729
DdeI CTNAG 2 cut(s) 36, 462
DinI GGCGCC 1 cut(s) 805
DpnI GATC 8 cut(s) 137, 497, 503, 519, 536, 558, 579, 758
DpnII GATC 8 cut(s) 135, 495, 501, 517, 534, 556, 577, 756
DrdI GACNNNNNNGTC 1 cut(s) 716
DseDI GACNNNNNNGTC 1 cut(s) 716
EaeI YGGCCR 3 cut(s) 380, 568, 679
EagI CGGCCG 1 cut(s) 568
Eam1104I CTCTTC 1 cut(s) 542
EarI CTCTTC 1 cut(s) 542
Ecl136II GAGCTC 1 cut(s) 331
EclXI CGGCCG 1 cut(s) 568
Eco147I AGGCCT 2 cut(s) 416, 672
Eco24I GRGCYC 1 cut(s) 333
Eco47I GGWCC 5 cut(s) 316, 351, 562, 583, 657
Eco52I CGGCCG 1 cut(s) 568
Eco53kI GAGCTC 1 cut(s) 331
Eco57I CTGAAG 1 cut(s) 500
Eco72I CACGTG 1 cut(s) 121
Eco88I CYCGRG 2 cut(s) 236, 391
EcoICRI GAGCTC 1 cut(s) 331
EcoO109I RGGNCCY 1 cut(s) 351
EcoRII CCWGG 2 cut(s) 312, 431
EcoT38I GRGCYC 1 cut(s) 333
EgeI GGCGCC 1 cut(s) 805
EheI GGCGCC 1 cut(s) 805
FaeI CATG 5 cut(s) 31, 283, 367, 460, 598
FaiI YATR 6 cut(s) 29, 281, 288, 365, 458, 596
FalI AAGNNNNNCTT 2 cut(s) 404, 436
FaqI GGGAC 5 cut(s) 53, 337, 409, 467, 566
FatI CATG 5 cut(s) 27, 279, 363, 456, 594
Fnu4HI GCNGC 6 cut(s) 41, 93, 221, 307, 571, 701
FokI GGATG 1 cut(s) 556
FriOI GRGCYC 1 cut(s) 333
Fsp4HI GCNGC 6 cut(s) 41, 93, 221, 307, 571, 701
GlaI GCGC 3 cut(s) 91, 768, 805
GluI GCNGC 6 cut(s) 41, 93, 221, 307, 571, 701
GsaI CCCAGC 1 cut(s) 608
GsuI CTGGAG 1 cut(s) 296
HaeII RGCGCY 1 cut(s) 807
HapII CCGG 6 cut(s) 171, 303, 383, 392, 632, 801
HgaI GACGC 3 cut(s) 96, 334, 406
HhaI GCGC 3 cut(s) 92, 769, 806
Hin1I GRCGYC 3 cut(s) 398, 621, 804
Hin1II CATG 5 cut(s) 31, 283, 367, 460, 598
Hin6I GCGC 3 cut(s) 90, 767, 804
HinP1I GCGC 3 cut(s) 90, 767, 804
HinfI GANTC 3 cut(s) 125, 251, 686
HpaII CCGG 6 cut(s) 171, 303, 383, 392, 632, 801
HphI GGTGA 3 cut(s) 133, 175, 328
Hpy166II GTNNAC 6 cut(s) 100, 118, 124, 201, 295, 657
Hpy188I TCNGA 3 cut(s) 449, 522, 539
Hpy188III TCNNGA 7 cut(s) 28, 499, 505, 532, 560, 581, 734
Hpy8I GTNNAC 6 cut(s) 100, 118, 124, 201, 295, 657
Hpy99I CGWCG 8 cut(s) 56, 65, 149, 266, 350, 623, 698, 713
HpyAV CCTTC 7 cut(s) 349, 397, 427, 444, 662, 709, 817
HpyCH4III ACNGT 4 cut(s) 115, 145, 601, 788
HpyCH4IV ACGT 3 cut(s) 63, 120, 621
HpyF10VI GCNNNNNNNGC 5 cut(s) 40, 306, 467, 610, 678
HpyF3I CTNAG 2 cut(s) 36, 462
HpySE526I ACGT 3 cut(s) 63, 120, 621
Hsp92I GRCGYC 3 cut(s) 398, 621, 804
Hsp92II CATG 5 cut(s) 31, 283, 367, 460, 598
HspAI GCGC 3 cut(s) 90, 767, 804
KasI GGCGCC 1 cut(s) 803
KflI GGGWCCC 1 cut(s) 351
Kzo9I GATC 8 cut(s) 135, 495, 501, 517, 534, 556, 577, 756
LmnI GCTCC 2 cut(s) 336, 837
MaeII ACGT 3 cut(s) 63, 120, 621
MaeIII GTNAC 1 cut(s) 155
MalI GATC 8 cut(s) 137, 497, 503, 519, 536, 558, 579, 758
MbiI CCGCTC 1 cut(s) 700
MboI GATC 8 cut(s) 135, 495, 501, 517, 534, 556, 577, 756
MboII GAAGA 7 cut(s) 250, 493, 559, 677, 826, 829, 832
MflI RGATCY 1 cut(s) 135
MhlI GDGCHC 3 cut(s) 333, 447, 793
Mly113I GGCGCC 1 cut(s) 804
MlyI GAGTC 3 cut(s) 119, 245, 680
MmeI TCCRAC 1 cut(s) 234
MroXI GAANNNNTTC 1 cut(s) 614
MslI CAYNNNNRTG 1 cut(s) 599
MspA1I CMGCKG 1 cut(s) 223
MspI CCGG 6 cut(s) 171, 303, 383, 392, 632, 801
MspR9I CCNGG 5 cut(s) 303, 314, 392, 393, 433
MvaI CCWGG 2 cut(s) 314, 433
MvnI CGCG 4 cut(s) 43, 90, 533, 767
MwoI GCNNNNNNNGC 5 cut(s) 40, 306, 467, 610, 678
NarI GGCGCC 1 cut(s) 804
NciI CCSGG 3 cut(s) 303, 392, 393
NdeII GATC 8 cut(s) 135, 495, 501, 517, 534, 556, 577, 756
NlaIII CATG 5 cut(s) 31, 283, 367, 460, 598
NlaIV GGNNCC 6 cut(s) 301, 352, 353, 763, 805, 839
NmeAIII GCCGAG 2 cut(s) 546, 726
NmuCI GTSAC 1 cut(s) 155
NruI TCGCGA 1 cut(s) 533
PagI TCATGA 1 cut(s) 27
PasI CCCWGGG 1 cut(s) 432
PceI AGGCCT 2 cut(s) 416, 672
PcsI WCGNNNNNNNCGW 2 cut(s) 60, 573
PdmI GAANNNNTTC 1 cut(s) 614
Pfl23II CGTACG 1 cut(s) 690
PflFI GACNNNGTC 1 cut(s) 65
PfoI TCCNGGA 1 cut(s) 312
PkrI GCNGC 6 cut(s) 42, 94, 222, 308, 572, 702
PleI GAGTC 3 cut(s) 119, 245, 680
PluTI GGCGCC 1 cut(s) 807
PmaCI CACGTG 1 cut(s) 121
PmlI CACGTG 1 cut(s) 121
PpsI GAGTC 3 cut(s) 119, 245, 680
Ppu21I YACGTR 1 cut(s) 121
PpuMI RGGWCCY 1 cut(s) 351
Psp124BI GAGCTC 1 cut(s) 333
Psp5II RGGWCCY 1 cut(s) 351
Psp6I CCWGG 2 cut(s) 312, 431
PspCI CACGTG 1 cut(s) 121
PspFI CCCAGC 1 cut(s) 604
PspGI CCWGG 2 cut(s) 312, 431
PspLI CGTACG 1 cut(s) 690
PspN4I GGNNCC 6 cut(s) 301, 352, 353, 763, 805, 839
PspPI GGNCC 7 cut(s) 246, 299, 316, 351, 562, 583, 657
PspPPI RGGWCCY 1 cut(s) 351
PsuI RGATCY 1 cut(s) 135
PsyI GACNNNGTC 1 cut(s) 65
RruI TCGCGA 1 cut(s) 533
RsaI GTAC 4 cut(s) 296, 368, 692, 730
RsaNI GTAC 4 cut(s) 295, 367, 691, 729
RseI CAYNNNNRTG 1 cut(s) 599
SacI GAGCTC 1 cut(s) 333
SatI GCNGC 6 cut(s) 41, 93, 221, 307, 571, 701
Sau3AI GATC 8 cut(s) 135, 495, 501, 517, 534, 556, 577, 756
Sau96I GGNCC 7 cut(s) 246, 299, 316, 351, 562, 583, 657
ScaI AGTACT 1 cut(s) 730
SchI GAGTC 3 cut(s) 119, 245, 680
ScrFI CCNGG 5 cut(s) 303, 314, 392, 393, 433
SduI GDGCHC 3 cut(s) 333, 447, 793
SfiI GGCCNNNNNGGCC 2 cut(s) 306, 678
SfoI GGCGCC 1 cut(s) 805
SinI GGWCC 5 cut(s) 316, 351, 562, 583, 657
SmaI CCCGGG 1 cut(s) 393
SmiMI CAYNNNNRTG 1 cut(s) 599
SmlI CTYRAG 1 cut(s) 194
SmoI CTYRAG 1 cut(s) 194
SseBI AGGCCT 2 cut(s) 416, 672
SspDI GGCGCC 1 cut(s) 803
SstI GAGCTC 1 cut(s) 333
StuI AGGCCT 2 cut(s) 416, 672
StyD4I CCNGG 5 cut(s) 301, 312, 390, 391, 431
TaaI ACNGT 4 cut(s) 115, 145, 601, 788
TaiI ACGT 3 cut(s) 66, 123, 624
TaqI TCGA 5 cut(s) 54, 60, 498, 516, 618
TatI WGTACW 1 cut(s) 728
TauI GCSGC 6 cut(s) 43, 95, 223, 309, 573, 703
TscAI CASTG 1 cut(s) 362
TseFI GTSAC 1 cut(s) 155
Tsp45I GTSAC 1 cut(s) 155
TspDTI ATGAA 4 cut(s) 16, 44, 303, 560
TspMI CCCGGG 1 cut(s) 391
TspRI CASTG 1 cut(s) 362
Tth111I GACNNNGTC 1 cut(s) 65
VpaK11BI GGWCC 5 cut(s) 316, 351, 562, 583, 657
XcmI CCANNNNNNNNNTGG 1 cut(s) 601
XmaI CCCGGG 1 cut(s) 391
XmnI GAANNNNTTC 1 cut(s) 614
ZraI GACGTC 1 cut(s) 622
ZrmI AGTACT 1 cut(s) 730
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.