FvH4_4g00230

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0008112
Physical Location & Seq
Reverse (-)
255648 .. 258499
2852 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g00230.t1

Sequence Viewer

Length: 375 bp
ATGGTGCTCGACGTTTCAATCGAGGAGATCGATCAGCCGGAGGTCATCCGCGCCTACCCCGCCAACGTCCCCAACCTCTTCACCGTCCACGTGGACTCCAAGCGCATCCTGACCGTCCTCATCGACCACGACGGCGTTGCCTCCAAGTGGCTCACCGAGCTCCTCAGCTCCAAATCCACTAACACCAAGCCTCCTCTCCTCGGCGCGCCTCCACCTTCCCTCCCGACTCCATCTTCATCAGCCTCGTCGCCGAGGAGCACCTCCCCTTCTACGGCCTTCGCCCCTATGACTCCACCGACCATTGCCTCTACCTCCTCACCCTCCACGTCGGCTTTCATTGTTTCGAAGACGACGATGACAGCGCAGACGAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

125

Amino Acids

13.03

Weight (kDa)

6.03

Isoelectric Point (pI)

64.03

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 51, 206
AciI CCGC 2 cut(s) 49, 60
AcvI CACGTG 1 cut(s) 91
AfiI CCNNNNNNNGG 3 cut(s) 147, 200, 271
AgsI TTSAA 1 cut(s) 18
AjiI CACGTC 1 cut(s) 327
AluBI AGCT 2 cut(s) 160, 168
AluI AGCT 2 cut(s) 160, 168
Alw21I GWGCWC 3 cut(s) 9, 162, 260
AoxI GGCC 1 cut(s) 273
AscI GGCGCGCC 1 cut(s) 204
AspLEI GCGC 5 cut(s) 53, 105, 206, 208, 364
AsuHPI GGTGA 3 cut(s) 73, 145, 309
AsuII TTCGAA 1 cut(s) 344
BanII GRGCYC 1 cut(s) 162
BbrPI CACGTG 1 cut(s) 91
BbsI GAAGAC 1 cut(s) 353
Bbv12I GWGCWC 3 cut(s) 9, 162, 260
BbvCI CCTCAGC 1 cut(s) 164
BccI CCATC 1 cut(s) 238
BceAI ACGGC 2 cut(s) 148, 288
BcgI CGANNNNNNTGC 2 cut(s) 119, 153
BmgBI CACGTC 1 cut(s) 327
BmsI GCATC 1 cut(s) 114
BpiI GAAGAC 1 cut(s) 353
Bpu10I CCTNAGC 1 cut(s) 164
Bpu14I TTCGAA 1 cut(s) 344
Bsa29I ATCGAT 1 cut(s) 30
BsaAI YACGTR 1 cut(s) 91
BsaJI CCNNGG 2 cut(s) 199, 251
BsaXI ACNNNNNCTCC 6 cut(s) 80, 110, 175, 204, 205, 234
Bsc4I CCNNNNNNNGG 3 cut(s) 147, 200, 271
Bse3DI GCAATG 1 cut(s) 300
BseCI ATCGAT 1 cut(s) 30
BseDI CCNNGG 2 cut(s) 199, 251
BseGI GGATG 2 cut(s) 45, 105
BseLI CCNNNNNNNGG 3 cut(s) 147, 200, 271
BseMI GCAATG 1 cut(s) 300
BseMII CTCAG 1 cut(s) 178
BsePI GCGCGC 1 cut(s) 204
BseRI GAGGAG 6 cut(s) 38, 152, 183, 188, 268, 304
Bsh1236I CGCG 2 cut(s) 51, 206
BshFI GGCC 1 cut(s) 275
BshVI ATCGAT 1 cut(s) 30
BsiHKAI GWGCWC 3 cut(s) 9, 162, 260
BsiSI CCGG 1 cut(s) 38
BslFI GGGAC 1 cut(s) 53
BslI CCNNNNNNNGG 3 cut(s) 147, 200, 271
BsmFI GGGAC 1 cut(s) 53
BsnI GGCC 1 cut(s) 275
Bsp119I TTCGAA 1 cut(s) 344
Bsp1286I GDGCHC 3 cut(s) 9, 162, 260
Bsp143I GATC 2 cut(s) 27, 31
BspACI CCGC 2 cut(s) 49, 60
BspANI GGCC 1 cut(s) 275
BspCNI CTCAG 1 cut(s) 177
BspDI ATCGAT 1 cut(s) 30
BspFNI CGCG 2 cut(s) 51, 206
BspT104I TTCGAA 1 cut(s) 344
BsrDI GCAATG 1 cut(s) 300
BssECI CCNNGG 2 cut(s) 199, 251
BssHII GCGCGC 1 cut(s) 204
BssMI GATC 2 cut(s) 27, 31
Bst4CI ACNGT 2 cut(s) 85, 115
Bst6I CTCTTC 1 cut(s) 83
BstBAI YACGTR 1 cut(s) 91
BstBI TTCGAA 1 cut(s) 344
BstC8I GCNNGC 1 cut(s) 206
BstDEI CTNAG 1 cut(s) 164
BstF5I GGATG 2 cut(s) 45, 105
BstFNI CGCG 2 cut(s) 51, 206
BstHHI GCGC 5 cut(s) 53, 105, 206, 208, 364
BstKTI GATC 2 cut(s) 30, 34
BstMBI GATC 2 cut(s) 27, 31
BstMWI GCNNNNNNNGC 2 cut(s) 59, 157
BstUI CGCG 2 cut(s) 51, 206
BstV2I GAAGAC 1 cut(s) 353
Bsu15I ATCGAT 1 cut(s) 30
BsuRI GGCC 1 cut(s) 275
BsuTUI ATCGAT 1 cut(s) 30
BtrI CACGTC 1 cut(s) 327
BtsCI GGATG 2 cut(s) 45, 105
Cac8I GCNNGC 1 cut(s) 206
CfoI GCGC 5 cut(s) 53, 105, 206, 208, 364
ClaI ATCGAT 1 cut(s) 30
CviJI RGCY 8 cut(s) 37, 151, 160, 168, 190, 242, 275, 332
CviKI_1 RGCY 8 cut(s) 37, 151, 160, 168, 190, 242, 275, 332
DdeI CTNAG 1 cut(s) 164
DpnI GATC 2 cut(s) 29, 33
DpnII GATC 2 cut(s) 27, 31
Eam1104I CTCTTC 1 cut(s) 83
EarI CTCTTC 1 cut(s) 83
Ecl136II GAGCTC 1 cut(s) 160
Eco24I GRGCYC 1 cut(s) 162
Eco53kI GAGCTC 1 cut(s) 160
Eco72I CACGTG 1 cut(s) 91
EcoICRI GAGCTC 1 cut(s) 160
EcoT38I GRGCYC 1 cut(s) 162
FaiI YATR 1 cut(s) 287
FaqI GGGAC 1 cut(s) 53
FauI CCCGC 1 cut(s) 67
FokI GGATG 2 cut(s) 32, 92
FriOI GRGCYC 1 cut(s) 162
GlaI GCGC 5 cut(s) 52, 104, 205, 207, 363
HaeIII GGCC 1 cut(s) 275
HapII CCGG 1 cut(s) 38
HhaI GCGC 5 cut(s) 53, 105, 206, 208, 364
Hin6I GCGC 5 cut(s) 51, 103, 204, 206, 362
HinP1I GCGC 5 cut(s) 51, 103, 204, 206, 362
HinfI GANTC 3 cut(s) 95, 226, 289
HpaII CCGG 1 cut(s) 38
HphI GGTGA 3 cut(s) 73, 145, 309
Hpy166II GTNNAC 2 cut(s) 88, 94
Hpy188III TCNNGA 2 cut(s) 109, 223
Hpy8I GTNNAC 2 cut(s) 88, 94
Hpy99I CGWCG 5 cut(s) 14, 134, 250, 331, 355
HpyAV CCTTC 3 cut(s) 225, 276, 286
HpyCH4III ACNGT 2 cut(s) 85, 115
HpyCH4IV ACGT 4 cut(s) 12, 66, 90, 326
HpyF10VI GCNNNNNNNGC 2 cut(s) 59, 157
HpyF3I CTNAG 1 cut(s) 164
HpySE526I ACGT 4 cut(s) 12, 66, 90, 326
HspAI GCGC 5 cut(s) 51, 103, 204, 206, 362
Kzo9I GATC 2 cut(s) 27, 31
LmnI GCTCC 3 cut(s) 165, 173, 255
LpnPI CCDG 2 cut(s) 51, 122
LweI GCATC 1 cut(s) 114
MaeII ACGT 4 cut(s) 12, 66, 90, 326
MalI GATC 2 cut(s) 29, 33
MboI GATC 2 cut(s) 27, 31
MboII GAAGA 3 cut(s) 70, 225, 358
MhlI GDGCHC 3 cut(s) 9, 162, 260
MluCI AATT 1 cut(s) 370
MlyI GAGTC 3 cut(s) 89, 220, 283
MspI CCGG 1 cut(s) 38
MvnI CGCG 2 cut(s) 51, 206
MwoI GCNNNNNNNGC 2 cut(s) 59, 157
NdeII GATC 2 cut(s) 27, 31
NmeAIII GCCGAG 2 cut(s) 180, 276
NspV TTCGAA 1 cut(s) 344
PalAI GGCGCGCC 1 cut(s) 204
PauI GCGCGC 1 cut(s) 204
PcsI WCGNNNNNNNCGW 3 cut(s) 18, 27, 350
PleI GAGTC 3 cut(s) 89, 220, 283
PmaCI CACGTG 1 cut(s) 91
PmlI CACGTG 1 cut(s) 91
PpsI GAGTC 3 cut(s) 89, 220, 283
Ppu21I YACGTR 1 cut(s) 91
Psp124BI GAGCTC 1 cut(s) 162
PspCI CACGTG 1 cut(s) 91
PteI GCGCGC 1 cut(s) 204
SacI GAGCTC 1 cut(s) 162
Sau3AI GATC 2 cut(s) 27, 31
SchI GAGTC 3 cut(s) 89, 220, 283
SduI GDGCHC 3 cut(s) 9, 162, 260
SfaNI GCATC 1 cut(s) 114
SfuI TTCGAA 1 cut(s) 344
SgsI GGCGCGCC 1 cut(s) 204
Sse9I AATT 1 cut(s) 370
SsiI CCGC 2 cut(s) 49, 60
SstI GAGCTC 1 cut(s) 162
TaaI ACNGT 2 cut(s) 85, 115
TaiI ACGT 4 cut(s) 15, 69, 93, 329
TaqI TCGA 5 cut(s) 9, 21, 30, 123, 344
TasI AATT 1 cut(s) 370
TspDTI ATGAA 2 cut(s) 225, 325
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.