Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb2

Orthogroup ID

OG0004157

Physical Location & Seq

Genomic Coordinates Forward (+)

1841265 .. 1849743

Gene Structure 8479 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_2g02240.t9

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3087 bp

ATGAGCTTCTCCGCCAAGCCTCAGCCACTCGCCCTCATCTCATGCACGCCTCACTCCCGCTCCCTCCGCCGCGACTTCGTCGGATGCCCCCACACCCTCCGCCCCCTCGCCGGCGGCCTCCGCTCCCTCCGCAAGAATCGCAACCTCGCCGGCGCCGGCGGACGTCAGAATCCTCCGTCGAGGTTGCTGATTAAAGCGTCGCTGAACTCTCACTCGATTCTGGTGTTTGTCGCCGTGGTGACGTTCTCGGCGGTGTCCGTTGTGTACTTGAACCGGTGGTTGAAGCCGAAGAAGAAGAGCAGTAATAGTGCTCCGGTTGGAGAAGATGGACGTATTGATTTTGATGCGTTGAAGGATGAGATTAGAGAGGCGAGGAAGGAGGAGGGAGAGGTTCAGGTGCTGCAGTTTCACAACTCGTCTGTGATTTCTGCGCAGGAGTCTCTTTCGCCGCTGGTGTTTGAGTCCACGGCGGTGCTTCAGCCGCTGCGCTTCCCCAAAGAGGTGACCGAGTTTGATAAAATCGACTCGCTTTTCGAGCTGCCGAATCTGATGGGCGACTCTGATTTTGGCTCTGTGACTGTAACTGATGATGAGGAGGAGGAAATCGTCACTGAGTCACCTTCTGTGTCTGGAAATGATGAGGAGAGTGTTGAAGTTGGTGAAGCTAATGGCTTTCGTTTTCTTAATGGAGAATCGGTGAGAGAAGAGATTCACATGTTCTATGAGGCCGAGAAGAATGAGATGAAGTTGGATGAGAAGAAGTTCTCTTCGTTTCTGAGAAATAACACCCTCACAAGATCAGATTCCTTTGGACAAGTTTCCCATCAGATTACTACAGAGAATGTAAAAGGCAAAATGCCTAATCACAAAGAAGGCCATGTCCGAAGCAGAGGAGACTTGGGAAACGGCAATGGATATGTAGCTGATACAGAACTAAGACATTTGGCTAAAAAGAACTCTAAGACTGTGCCTCAACCAAATGGAATACAGACAAGTGACACACATTATATTTCCGAGCAATTAAGTGCTTATCATCGTTTGCTGAAGGACGGGAGGTTATCCGACTCTTTAAGGTTGCTTGAAGATTTGGAGAAAAAGGATTTGTTGGATATGAATAAGGTTTATCATGGAAGGTTTTTTGAAACCTGCAAGAAGAAAAAAGCTGTTGATCAAGCTTTTCGTTTCATCAAACTTATTCCAAATCCAACAATGAGTACATATAATATGCTTATGTCTGTCTGTGCGAGTTCTCAAGATTCAGAGGGAGCTTTCAATGTTCTGGGACTTGTCCGGGAGGCTGGGCTGAGAGTTGATTGCAAACTTTACACCACTCTGATATCAACATGTGCTAAAAGTGGAAAAGTCTACACCATGTTTGACGTTTTCCATGAGATGGTTAGTGCTGGAGTAGAACCAAATGTACACACATATGGGGCACTGATCGACGGATGTGGTAGAGCTGGGGAAGTGGCAAAGGCATTTGGTGCCTATGGGATAATGAGGTCTAAGAAAGTGAAGCCAGATCGAGTTGTATTCAATGCGCTTATTACTGCATGTGGTCAATCAGGAGCAGTTGACCGTGCTTTTGATGTCTTAGAAGAAATGAAGGCAGAGACACAACCTATAGAACCTGACCACACAACTATTGGTGCACTGATAAAAGCATGTGCAAATGCTGGCCAGGTTGAAAGGGCACGAGAAGTGTATAAGATGATTCATAAGTACAAGATTAAGGGCACTTCTGAGGTTTACACCATAGCCGTTAATTGTTGCAGCCAGACTGCTGATTGGGAGTTTGCTTGCACTGTATATGACTACATGAAAAAGAATGGTGTGACCCCTGATGAGGTGTTCCTCAGTGCATTAATAGATGTTGCTGGGCATGCTGGAAAGCTAGATGCTGCCTTTGAAATCATACAGGATGCAAGTAATCGAGGAATACAAGTTGGAACTGTATCATATAGCTCATTGATGGGTGCCTGTAGCAATGCCAAAAATTGGCAGAAAGCACTGGAGCTCTATGAGGATCTTAAGTCTGCTAAAATTGAGCAGACTGTTTCAACTATCAATGCTTTGATTACGGCCCTGTGTGATGGGGATCAACTACAGAAGGCTATGGAAGTTCTGTCGGAAATGAAGAGCATAGGACTGCGTCCAAACAGTATAACATACTCCATACTTGTAGTGGCAAGTGAAAAGAAGGATGATCTAGAAGCTGGCTTGATGCTCCTTTCCCAAGCTGAAATGGATAAAGTTGTTCCAAACCTCGTCATGTGTAGATGTATTATAGGCATGTGTTTACGGAGATCTGAGAAGGCATGTACCCTTGGTGAACCTGTCTTGCCACTTGACTCAGGCCGACCACAAGTGGATAGTAAATGGTCATCAGTGGCCTTAATGGTCTACAGGAAAACTATTGTAGCTGGTACCACTCCTACCATTGAAATTATATCACAAGTTTTGGGATGCTTGCAGCTCCCTTACGATGCAGCATCGAAGAATAGAGTTATTGAGAATCTTGGTGTAACGGCTGACATGTCTAGAGCTTCAAAACTCTGTTCACTGATAGATGGGTTTGGTGAATATGACCCTCGTGCATTTTCATTACTTGAGGAAGCTGCTTCACTTGGAATTGTTCCATGTGTATCCTTCAAAGCTAGTCCCATTGTTGTTGATGCAAAGAAGTTGCAACTTCATACTGCTGAGGTCTACATCTTGACTGTTCTGAGAGGTCTCAAACATCGGCTTGCTGCTGGTGCCAAGTTGCCCAACATGACCATATTACTGCCTGTAGAGAAGACACAAATTTTGTCTCCCAAGGGGAAGTTGAAGACAATCAACCTTTCAGGAAGGGTTGGTCAATCAGTTGCATCATTATTAAGAAGGCTTGGGATAGATTACCAAGGAAATGAATCACGTGGAAAGATAAGAATCAGTGGGTTAACCTTGAAAAGATGGTTTCAACCAAAGCTTGCTTCACCATTCACTGGGAAACTGGCAGAATTAGGCTCATCCCAGTTGCGTCTGGGGAAAGGAATCATGCATCAGCAGCGCAATATTCGAACTGGAAACCTTTCCTTGGATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009507 GO:0009536 GO:0009892 GO:0009893 GO:0009894 GO:0009895 GO:0009987 GO:0010468 GO:0010604 GO:0010605 GO:0010608 GO:0010628 GO:0016070 GO:0016787 GO:0016788 GO:0019219 GO:0019222 GO:0031323 GO:0031324 GO:0031329 GO:0031330 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043487 GO:0043488 GO:0043489 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0045934 GO:0046483 GO:0048255 GO:0048518 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0060255 GO:0061013 GO:0065007 GO:0065008 GO:0071704 GO:0080090 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363 GO:1902369 GO:1902373 GO:1903311 GO:1903312

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2 PPR_3

Protein Analysis

1029

Amino Acids

112.97

Weight (kDa)

8.5

Isoelectric Point (pI)

43.0

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_long	PF17177	3 - 97	1.7e-07	Pentacotripeptide-repeat region of PRORP
TPR_24	PF23276	18 - 142	9.3e-10	Fungal tetratrico peptide repeats
PPR_long	PF17177	48 - 200	9.5e-15	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	104 - 162	5e-08	Pentatricopeptide repeat domain
PPR_2	PF13041	116 - 164	8.2e-09	PPR repeat family
PPR_3	PF13812	140 - 194	2.3e-07	Pentatricopeptide repeat domain
PPR_2	PF13041	151 - 194	1.6e-11	PPR repeat family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0004157)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G34830
fragaria_vesca	FvH4_2g02240 FvH4_2g02240 FvH4_2g02240 FvH4_2g02240 FvH4_2g02240 FvH4_2g02240 FvH4_2g02240 FvH4_2g02240 FvH4_2g02240 FvH4_2g02240 FvH4_2g02240 FvH4_2g02240 FvH4_2g02240
malus_domestica	MD05G1113900.v1.1
prunus_persica	Prupe.8G158300_v2.0.a1 Prupe.8G158300_v2.0.a1 Prupe.8G158300_v2.0.a1
pyrus_communis	pycom05g10870 pycom10g10060
rosa_chinensis	RchiOBHm_Chr6g0245931
rosa_laevigata	RLG00000015285
rosa_multiflora	Rmu_co8211740.1_g000001 Rmu_sc0005570.1_g000001 Rmu_sc0018377.1_g000001 Rmu_sc0018378.1_g000004
rosa_roxburghii	Rroxscaffold_7G00215060
rosa_rugosa	Rorug05G0517500
rosa_samantha	Rh6AG031800 Rh6BG028200 Rh6CG028200 Rh6DG027500
rosa_wichuraiana	Rw6G002660

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	166
Acc16I	TGCGCA	1 cut(s)	432
Acc36I	ACCTGC	1 cut(s)	1154
Acc65I	GGTACC	1 cut(s)	2426
AccB1I	GGYRCC	5 cut(s)	152, 1484, 1976, 2426, 2756
AccB7I	CCANNNNNTGG	3 cut(s)	1393, 1998, 2987
AccBSI	CCGCTC	2 cut(s)	60, 123
AccI	GTMKAC	3 cut(s)	1365, 2403, 2709
AccII	CGCG	1 cut(s)	72
AclWI	GGATC	2 cut(s)	2034, 2106
AcoI	YGGCCR	1 cut(s)	1678
AcsI	RAATTY	1 cut(s)	2805
AcuI	CTGAAG	2 cut(s)	461, 1064
AcvI	CACGTG	1 cut(s)	2918
AcyI	GRCGYC	2 cut(s)	153, 163
AfaI	GTAC	6 cut(s)	266, 1216, 1422, 1724, 2323, 2428
AfiI	CCNNNNNNNGG	7 cut(s)	110, 499, 1393, 1846, 1998, 2987, 3079
AflII	CTTAAG	1 cut(s)	2030
AflIII	ACRYGT	3 cut(s)	714, 1343, 2535
AgeI	ACCGGT	1 cut(s)	273
AjnI	CCWGG	1 cut(s)	1680
AjuI	GAANNNNNNNTTGG	2 cut(s)	2959, 2991
AleI	CACNNNNGTG	1 cut(s)	470
Alw21I	GWGCWC	3 cut(s)	313, 1654, 2019
Alw26I	GTCTC	5 cut(s)	444, 888, 1607, 2738, 2817
Alw44I	GTGCAC	1 cut(s)	1650
AlwI	GGATC	2 cut(s)	2034, 2106
AlwNI	CAGNNNCTG	2 cut(s)	400, 484
AoxI	GGCC	7 cut(s)	115, 726, 874, 1678, 2082, 2356, 2391
ApaLI	GTGCAC	1 cut(s)	1650
ApoI	RAATTY	1 cut(s)	2805
ArsI	GACNNNNNNTTYG	8 cut(s)	515, 547, 548, 580, 1568, 1600, 2791, 2823
AseI	ATTAAT	1 cut(s)	1865
AsiGI	ACCGGT	1 cut(s)	273
Asp700I	GAANNNNTTC	3 cut(s)	708, 761, 3073
Asp718I	GGTACC	1 cut(s)	2426
AspLEI	GCGC	5 cut(s)	155, 433, 489, 1543, 3054
AspS9I	GGNCC	1 cut(s)	2083
AsuC2I	CCSGG	1 cut(s)	1292
AsuHPI	GGTGA	8 cut(s)	250, 514, 609, 671, 709, 2342, 2591, 2970
AsuII	TTCGAA	1 cut(s)	3061
BaeGI	GKGCMC	4 cut(s)	1438, 1654, 1696, 1739
BalI	TGGCCA	1 cut(s)	1680
BanI	GGYRCC	5 cut(s)	152, 1484, 1976, 2426, 2756
BanII	GRGCYC	1 cut(s)	2019
BauI	CACGAG	2 cut(s)	1695, 2592
BbrPI	CACGTG	1 cut(s)	2918
BbsI	GAAGAC	2 cut(s)	2804, 2837
Bbv12I	GWGCWC	3 cut(s)	313, 1654, 2019
BbvCI	CCTCAGC	2 cut(s)	21, 2703
BccI	CCATC	8 cut(s)	320, 544, 831, 1387, 1966, 2087, 2564, 2949
BceAI	ACGGC	6 cut(s)	218, 483, 922, 1745, 2097, 2544
BciT130I	CCWGG	1 cut(s)	1682
BciVI	GTATCC	1 cut(s)	2656
BclI	TGATCA	1 cut(s)	1168
BcnI	CCSGG	1 cut(s)	1292
BcoDI	GTCTC	5 cut(s)	444, 888, 1607, 2738, 2817
BfaI	CTAG	4 cut(s)	1895, 2210, 2541, 2658
BfmI	CTRYAG	7 cut(s)	401, 834, 1623, 1981, 2105, 2404, 2790
BfoI	RGCGCY	1 cut(s)	156
BfrI	CTTAAG	1 cut(s)	2030
BfuAI	ACCTGC	1 cut(s)	1154
BfuI	GTATCC	1 cut(s)	2656
BglII	AGATCT	1 cut(s)	2306
Bme1390I	CCNGG	2 cut(s)	1292, 1682
BmgT120I	GGNCC	1 cut(s)	2083
BmiI	GGNNCC	5 cut(s)	154, 1486, 1978, 2428, 2758
BmrFI	CCNGG	2 cut(s)	1292, 1682
BmrI	ACTGGG	2 cut(s)	2997, 3010
BmuI	ACTGGG	2 cut(s)	2997, 3010
BpiI	GAAGAC	2 cut(s)	2804, 2837
BpmI	CTGGAG	2 cut(s)	1425, 2033
Bpu10I	CCTNAGC	2 cut(s)	21, 2703
Bpu14I	TTCGAA	1 cut(s)	3061
BpuEI	CTTGAG	2 cut(s)	1236, 2630
BpuMI	CCSGG	1 cut(s)	1292
BsaAI	YACGTR	1 cut(s)	2918
BsaBI	GATNNNNATC	2 cut(s)	2097, 2930
BsaHI	GRCGYC	2 cut(s)	153, 163
BsaI	GGTCTC	1 cut(s)	2738
BsaJI	CCNNGG	6 cut(s)	234, 465, 2326, 2817, 2902, 3078
BsaWI	WCCGGW	2 cut(s)	273, 313
BsaXI	ACNNNNNCTCC	4 cut(s)	44, 74, 681, 711
Bsc4I	CCNNNNNNNGG	7 cut(s)	110, 499, 1393, 1846, 1998, 2987, 3079
Bse118I	RCCGGY	4 cut(s)	110, 149, 155, 273
Bse1I	ACTGG	5 cut(s)	2016, 2992, 3000, 3016, 3070
Bse3DI	GCAATG	2 cut(s)	916, 1993
Bse8I	GATNNNNATC	2 cut(s)	2097, 2930
BseBI	CCWGG	1 cut(s)	1682
BseDI	CCNNGG	6 cut(s)	234, 465, 2326, 2817, 2902, 3078
BseGI	GGATG	8 cut(s)	89, 361, 757, 1454, 1927, 2209, 2471, 3011
BseJI	GATNNNNATC	2 cut(s)	2097, 2930
BseLI	CCNNNNNNNGG	7 cut(s)	110, 499, 1393, 1846, 1998, 2987, 3079
BseMI	GCAATG	2 cut(s)	916, 1993
BseNI	ACTGG	5 cut(s)	2016, 2992, 3000, 3016, 3070
BseRI	GAGGAG	5 cut(s)	395, 608, 611, 656, 906
BseSI	GKGCMC	4 cut(s)	1438, 1654, 1696, 1739
BseYI	CCCAGC	3 cut(s)	1298, 1460, 1877
Bsh1236I	CGCG	1 cut(s)	72
BshFI	GGCC	7 cut(s)	117, 728, 876, 1680, 2084, 2358, 2393
BshNI	GGYRCC	5 cut(s)	152, 1484, 1976, 2426, 2756
BshTI	ACCGGT	1 cut(s)	273
BsiHKAI	GWGCWC	3 cut(s)	313, 1654, 2019
BsiSI	CCGG	6 cut(s)	111, 150, 156, 274, 314, 1291
BslFI	GGGAC	2 cut(s)	1296, 2646
BslI	CCNNNNNNNGG	7 cut(s)	110, 499, 1393, 1846, 1998, 2987, 3079
BsmAI	GTCTC	5 cut(s)	444, 888, 1607, 2738, 2817
BsmFI	GGGAC	2 cut(s)	1296, 2646
BsnI	GGCC	7 cut(s)	117, 728, 876, 1680, 2084, 2358, 2393
Bso31I	GGTCTC	1 cut(s)	2738
Bsp119I	TTCGAA	1 cut(s)	3061
Bsp1286I	GDGCHC	6 cut(s)	313, 1438, 1654, 1696, 1739, 2019
Bsp1407I	TGTACA	1 cut(s)	1420
Bsp143I	GATC	8 cut(s)	797, 1168, 1440, 1522, 2026, 2098, 2206, 2306
BspANI	GGCC	7 cut(s)	117, 728, 876, 1680, 2084, 2358, 2393
BspFNI	CGCG	1 cut(s)	72
BspLI	GGNNCC	5 cut(s)	154, 1486, 1978, 2428, 2758
BspMAI	CTGCAG	1 cut(s)	405
BspMI	ACCTGC	1 cut(s)	1154
BspPI	GGATC	2 cut(s)	2034, 2106
BspQI	GCTCTTC	2 cut(s)	290, 2132
BspT104I	TTCGAA	1 cut(s)	3061
BspT107I	GGYRCC	5 cut(s)	152, 1484, 1976, 2426, 2756
BspTI	CTTAAG	1 cut(s)	2030
BspTNI	GGTCTC	1 cut(s)	2738
BsrBI	CCGCTC	2 cut(s)	60, 123
BsrDI	GCAATG	2 cut(s)	916, 1993
BsrFI	RCCGGY	4 cut(s)	110, 149, 155, 273
BsrGI	TGTACA	1 cut(s)	1420
BsrI	ACTGG	5 cut(s)	2016, 2992, 3000, 3016, 3070
BssAI	RCCGGY	4 cut(s)	110, 149, 155, 273
BssECI	CCNNGG	6 cut(s)	234, 465, 2326, 2817, 2902, 3078
BssMI	GATC	8 cut(s)	797, 1168, 1440, 1522, 2026, 2098, 2206, 2306
BssNI	GRCGYC	2 cut(s)	153, 163
BssSI	CACGAG	2 cut(s)	1695, 2592
BssT1I	CCWWGG	4 cut(s)	2326, 2817, 2902, 3078
Bst2BI	CACGAG	2 cut(s)	1695, 2592
Bst2UI	CCWGG	1 cut(s)	1682
Bst4CI	ACNGT	8 cut(s)	580, 967, 1580, 1807, 1954, 2056, 2162, 2722
Bst6I	CTCTTC	4 cut(s)	290, 699, 772, 2132
BstACI	GRCGYC	2 cut(s)	153, 163
BstAFI	CTTAAG	1 cut(s)	2030
BstAPI	GCANNNNNTGC	1 cut(s)	1484
BstAUI	TGTACA	1 cut(s)	1420
BstBAI	YACGTR	1 cut(s)	2918
BstBI	TTCGAA	1 cut(s)	3061
BstDSI	CCRYGG	2 cut(s)	234, 465
BstEII	GGTNACC	1 cut(s)	502
BstF5I	GGATG	8 cut(s)	89, 361, 757, 1454, 1927, 2209, 2471, 3011
BstFNI	CGCG	1 cut(s)	72
BstH2I	RGCGCY	1 cut(s)	156
BstHHI	GCGC	5 cut(s)	155, 433, 489, 1543, 3054
BstKTI	GATC	8 cut(s)	800, 1171, 1443, 1525, 2029, 2101, 2209, 2309
BstMAI	GTCTC	5 cut(s)	444, 888, 1607, 2738, 2817
BstMBI	GATC	8 cut(s)	797, 1168, 1440, 1522, 2026, 2098, 2206, 2306
BstNI	CCWGG	1 cut(s)	1682
BstNSI	RCATGY	8 cut(s)	718, 1347, 1557, 1668, 1886, 2296, 2322, 2539
BstPI	GGTNACC	1 cut(s)	502
BstSCI	CCNGG	2 cut(s)	1290, 1680
BstSFI	CTRYAG	7 cut(s)	401, 834, 1623, 1981, 2105, 2404, 2790
BstSLI	GKGCMC	4 cut(s)	1438, 1654, 1696, 1739
BstUI	CGCG	1 cut(s)	72
BstV2I	GAAGAC	2 cut(s)	2804, 2837
BstX2I	RGATCY	2 cut(s)	2026, 2306
BstYI	RGATCY	2 cut(s)	2026, 2306
BsuI	GTATCC	1 cut(s)	2656
BsuRI	GGCC	7 cut(s)	117, 728, 876, 1680, 2084, 2358, 2393
BtgI	CCRYGG	2 cut(s)	234, 465
BtsCI	GGATG	8 cut(s)	89, 361, 757, 1454, 1927, 2209, 2471, 3011
BveI	ACCTGC	1 cut(s)	1154
CaiI	CAGNNNCTG	2 cut(s)	400, 484
CfoI	GCGC	5 cut(s)	155, 433, 489, 1543, 3054
Cfr10I	RCCGGY	4 cut(s)	110, 149, 155, 273
Cfr13I	GGNCC	1 cut(s)	2083
CseI	GACGC	3 cut(s)	186, 2141, 3011
Csp6I	GTAC	6 cut(s)	265, 1215, 1421, 1723, 2322, 2427
CspAI	ACCGGT	1 cut(s)	273
CviQI	GTAC	6 cut(s)	265, 1215, 1421, 1723, 2322, 2427
DinI	GGCGCC	1 cut(s)	154
DpnI	GATC	8 cut(s)	799, 1170, 1442, 1524, 2028, 2100, 2208, 2308
DpnII	GATC	8 cut(s)	797, 1168, 1440, 1522, 2026, 2098, 2206, 2306
EaeI	YGGCCR	1 cut(s)	1678
Eam1104I	CTCTTC	4 cut(s)	290, 699, 772, 2132
EarI	CTCTTC	4 cut(s)	290, 699, 772, 2132
EciI	GGCGGA	3 cut(s)	56, 89, 174
Ecl136II	GAGCTC	1 cut(s)	2017
Eco130I	CCWWGG	4 cut(s)	2326, 2817, 2902, 3078
Eco24I	GRGCYC	1 cut(s)	2019
Eco31I	GGTCTC	1 cut(s)	2738
Eco32I	GATATC	1 cut(s)	1338
Eco53kI	GAGCTC	1 cut(s)	2017
Eco57I	CTGAAG	2 cut(s)	461, 1064
Eco72I	CACGTG	1 cut(s)	2918
Eco91I	GGTNACC	1 cut(s)	502
EcoICRI	GAGCTC	1 cut(s)	2017
EcoO65I	GGTNACC	1 cut(s)	502
EcoRII	CCWGG	1 cut(s)	1680
EcoRV	GATATC	1 cut(s)	1338
EcoT14I	CCWWGG	4 cut(s)	2326, 2817, 2902, 3078
EcoT22I	ATGCAT	1 cut(s)	3045
EcoT38I	GRGCYC	1 cut(s)	2019
EgeI	GGCGCC	1 cut(s)	154
EheI	GGCGCC	1 cut(s)	154
ErhI	CCWWGG	4 cut(s)	2326, 2817, 2902, 3078
FaqI	GGGAC	2 cut(s)	1296, 2646
FauI	CCCGC	1 cut(s)	65
FauNDI	CATATG	1 cut(s)	1429
FbaI	TGATCA	1 cut(s)	1168
FblI	GTMKAC	3 cut(s)	1365, 2403, 2709
FokI	GGATG	8 cut(s)	96, 368, 764, 1461, 1934, 2216, 2478, 2998
FriOI	GRGCYC	1 cut(s)	2019
FspBI	CTAG	4 cut(s)	1895, 2210, 2541, 2658
FspI	TGCGCA	1 cut(s)	432
GlaI	GCGC	5 cut(s)	154, 432, 488, 1542, 3053
GsaI	CCCAGC	3 cut(s)	1302, 1464, 1881
GsuI	CTGGAG	2 cut(s)	1425, 2033
HaeII	RGCGCY	1 cut(s)	156
HaeIII	GGCC	7 cut(s)	117, 728, 876, 1680, 2084, 2358, 2393
HapII	CCGG	6 cut(s)	111, 150, 156, 274, 314, 1291
HgaI	GACGC	3 cut(s)	186, 2141, 3011
HhaI	GCGC	5 cut(s)	155, 433, 489, 1543, 3054
Hin1I	GRCGYC	2 cut(s)	153, 163
Hin6I	GCGC	5 cut(s)	153, 431, 487, 1541, 3052
HinP1I	GCGC	5 cut(s)	153, 431, 487, 1541, 3052
HincII	GTYRAC	2 cut(s)	1576, 2943
HindII	GTYRAC	2 cut(s)	1576, 2943
HindIII	AAGCTT	2 cut(s)	1173, 2969
HpaI	GTTAAC	1 cut(s)	2943
HpaII	CCGG	6 cut(s)	111, 150, 156, 274, 314, 1291
HphI	GGTGA	8 cut(s)	250, 514, 609, 671, 709, 2342, 2591, 2970
Hpy188III	TCNNGA	7 cut(s)	630, 1253, 1566, 2210, 2541, 2716, 2847
Hpy99I	CGWCG	4 cut(s)	83, 181, 202, 1448
HpyCH4III	ACNGT	8 cut(s)	580, 967, 1580, 1807, 1954, 2056, 2162, 2722
HpyCH4IV	ACGT	5 cut(s)	163, 242, 331, 1380, 2917
HpySE526I	ACGT	5 cut(s)	163, 242, 331, 1380, 2917
Hsp92I	GRCGYC	2 cut(s)	153, 163
HspAI	GCGC	5 cut(s)	153, 431, 487, 1541, 3052
KasI	GGCGCC	1 cut(s)	152
KpnI	GGTACC	1 cut(s)	2430
KroI	GCCGGC	3 cut(s)	110, 149, 155
KroNI	GCCGGC	3 cut(s)	112, 151, 157
Ksp22I	TGATCA	1 cut(s)	1168
KspAI	GTTAAC	1 cut(s)	2943
Kzo9I	GATC	8 cut(s)	797, 1168, 1440, 1522, 2026, 2098, 2206, 2306
LguI	GCTCTTC	2 cut(s)	290, 2132
LmnI	GCTCC	8 cut(s)	65, 128, 316, 1265, 1568, 2014, 2232, 2481
MaeI	CTAG	4 cut(s)	1895, 2210, 2541, 2658
MaeII	ACGT	5 cut(s)	163, 242, 331, 1380, 2917
MaeIII	GTNAC	9 cut(s)	238, 502, 574, 580, 607, 615, 995, 1834, 2524
MalI	GATC	8 cut(s)	799, 1170, 1442, 1524, 2028, 2100, 2208, 2308
MbiI	CCGCTC	2 cut(s)	60, 123
MboI	GATC	8 cut(s)	797, 1168, 1440, 1522, 2026, 2098, 2206, 2306
MflI	RGATCY	2 cut(s)	2026, 2306
MhlI	GDGCHC	6 cut(s)	313, 1438, 1654, 1696, 1739, 2019
MlsI	TGGCCA	1 cut(s)	1680
MluCI	AATT	8 cut(s)	1019, 1765, 1996, 2043, 2445, 2631, 2805, 3002
MluNI	TGGCCA	1 cut(s)	1680
Mly113I	GGCGCC	1 cut(s)	153
MlyI	GAGTC	7 cut(s)	446, 470, 518, 551, 623, 1058, 2345
MmeI	TCCRAC	8 cut(s)	61, 298, 729, 1086, 1086, 1229, 1927, 2109
Mox20I	TGGCCA	1 cut(s)	1680
Mph1103I	ATGCAT	1 cut(s)	3045
MreI	CGCCGGCG	3 cut(s)	110, 149, 155
MroNI	GCCGGC	3 cut(s)	110, 149, 155
MroXI	GAANNNNTTC	3 cut(s)	708, 761, 3073
MscI	TGGCCA	1 cut(s)	1680
MslI	CAYNNNNRTG	2 cut(s)	470, 1428
Msp20I	TGGCCA	1 cut(s)	1680
MspA1I	CMGCKG	2 cut(s)	451, 484
MspCI	CTTAAG	1 cut(s)	2030
MspI	CCGG	6 cut(s)	111, 150, 156, 274, 314, 1291
MspR9I	CCNGG	2 cut(s)	1292, 1682
MvaI	CCWGG	1 cut(s)	1682
MvnI	CGCG	1 cut(s)	72
NaeI	GCCGGC	3 cut(s)	112, 151, 157
NarI	GGCGCC	1 cut(s)	153
NciI	CCSGG	1 cut(s)	1292
NdeI	CATATG	1 cut(s)	1429
NdeII	GATC	8 cut(s)	797, 1168, 1440, 1522, 2026, 2098, 2206, 2306
NgoMIV	GCCGGC	3 cut(s)	110, 149, 155
NlaIV	GGNNCC	5 cut(s)	154, 1486, 1978, 2428, 2758
NmeAIII	GCCGAG	2 cut(s)	227, 754
NmuCI	GTSAC	7 cut(s)	238, 502, 574, 607, 615, 995, 1834
NsbI	TGCGCA	1 cut(s)	432
NsiI	ATGCAT	1 cut(s)	3045
NspI	RCATGY	8 cut(s)	718, 1347, 1557, 1668, 1886, 2296, 2322, 2539
NspV	TTCGAA	1 cut(s)	3061
OliI	CACNNNNGTG	1 cut(s)	470
PaeI	GCATGC	1 cut(s)	1886
PciI	ACATGT	3 cut(s)	714, 1343, 2535
PciSI	GCTCTTC	2 cut(s)	290, 2132
PdiI	GCCGGC	3 cut(s)	112, 151, 157
PdmI	GAANNNNTTC	3 cut(s)	708, 761, 3073
PflFI	GACNNNGTC	2 cut(s)	77, 2151
PflMI	CCANNNNNTGG	3 cut(s)	1393, 1998, 2987
PfoI	TCCNGGA	1 cut(s)	1290
PinAI	ACCGGT	1 cut(s)	273
PleI	GAGTC	7 cut(s)	445, 469, 518, 551, 622, 1058, 2345
PluTI	GGCGCC	1 cut(s)	156
PmaCI	CACGTG	1 cut(s)	2918
PmlI	CACGTG	1 cut(s)	2918
PpsI	GAGTC	7 cut(s)	445, 469, 518, 551, 622, 1058, 2345
Ppu21I	YACGTR	1 cut(s)	2918
PscI	ACATGT	3 cut(s)	714, 1343, 2535
PshBI	ATTAAT	1 cut(s)	1865
Psp124BI	GAGCTC	1 cut(s)	2019
Psp6I	CCWGG	1 cut(s)	1680
PspCI	CACGTG	1 cut(s)	2918
PspEI	GGTNACC	1 cut(s)	502
PspFI	CCCAGC	3 cut(s)	1298, 1460, 1877
PspGI	CCWGG	1 cut(s)	1680
PspN4I	GGNNCC	5 cut(s)	154, 1486, 1978, 2428, 2758
PspPI	GGNCC	1 cut(s)	2083
PsrI	GAACNNNNNNTAC	2 cut(s)	1404, 1436
PstI	CTGCAG	1 cut(s)	405
PstNI	CAGNNNCTG	2 cut(s)	400, 484
PsuI	RGATCY	2 cut(s)	2026, 2306
PsyI	GACNNNGTC	2 cut(s)	77, 2151
RsaI	GTAC	6 cut(s)	266, 1216, 1422, 1724, 2323, 2428
RsaNI	GTAC	6 cut(s)	265, 1215, 1421, 1723, 2322, 2427
RseI	CAYNNNNRTG	2 cut(s)	470, 1428
SacI	GAGCTC	1 cut(s)	2019
SapI	GCTCTTC	2 cut(s)	290, 2132
Sau3AI	GATC	8 cut(s)	797, 1168, 1440, 1522, 2026, 2098, 2206, 2306
Sau96I	GGNCC	1 cut(s)	2083
SchI	GAGTC	7 cut(s)	446, 470, 518, 551, 623, 1058, 2345
ScrFI	CCNGG	2 cut(s)	1292, 1682
SduI	GDGCHC	6 cut(s)	313, 1438, 1654, 1696, 1739, 2019
SfcI	CTRYAG	7 cut(s)	401, 834, 1623, 1981, 2105, 2404, 2790
SfoI	GGCGCC	1 cut(s)	154
SfuI	TTCGAA	1 cut(s)	3061
SgrAI	CRCCGGYG	3 cut(s)	110, 149, 155
SmiMI	CAYNNNNRTG	2 cut(s)	470, 1428
SmlI	CTYRAG	3 cut(s)	1251, 2030, 2609
SmoI	CTYRAG	3 cut(s)	1251, 2030, 2609
SphI	GCATGC	1 cut(s)	1886
Sse9I	AATT	8 cut(s)	1019, 1765, 1996, 2043, 2445, 2631, 2805, 3002
SspDI	GGCGCC	1 cut(s)	152
SspI	AATATT	1 cut(s)	3058
SspMI	CTAG	4 cut(s)	1895, 2210, 2541, 2658
SstI	GAGCTC	1 cut(s)	2019
StyD4I	CCNGG	2 cut(s)	1290, 1680
StyI	CCWWGG	4 cut(s)	2326, 2817, 2902, 3078
TaaI	ACNGT	8 cut(s)	580, 967, 1580, 1807, 1954, 2056, 2162, 2722
TaiI	ACGT	5 cut(s)	166, 245, 334, 1383, 2920
TaqI	TCGA	9 cut(s)	179, 215, 522, 534, 1443, 1525, 1933, 2495, 3061
TaqII	GACCGA	1 cut(s)	521
TasI	AATT	8 cut(s)	1019, 1765, 1996, 2043, 2445, 2631, 2805, 3002
TatI	WGTACW	4 cut(s)	264, 1214, 1420, 1722
TauI	GCSGC	4 cut(s)	72, 117, 451, 484
TseFI	GTSAC	7 cut(s)	238, 502, 574, 607, 615, 995, 1834
Tsp45I	GTSAC	7 cut(s)	238, 502, 574, 607, 615, 995, 1834
TspGWI	ACGGA	4 cut(s)	165, 247, 1461, 2317
Tth111I	GACNNNGTC	2 cut(s)	77, 2151
Van91I	CCANNNNNTGG	3 cut(s)	1393, 1998, 2987
Vha464I	CTTAAG	1 cut(s)	2030
VneI	GTGCAC	1 cut(s)	1650
VspI	ATTAAT	1 cut(s)	1865
XapI	RAATTY	1 cut(s)	2805
XbaI	TCTAGA	2 cut(s)	2209, 2540
XceI	RCATGY	8 cut(s)	718, 1347, 1557, 1668, 1886, 2296, 2322, 2539
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1643, 2182
XmiI	GTMKAC	3 cut(s)	1365, 2403, 2709
XmnI	GAANNNNTTC	3 cut(s)	708, 761, 3073
XspI	CTAG	4 cut(s)	1895, 2210, 2541, 2658
ZraI	GACGTC	1 cut(s)	164
Zsp2I	ATGCAT	1 cut(s)	3045

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.