Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0004157

Physical Location & Seq

Genomic Coordinates Reverse (-)

23260020 .. 23269348

Gene Structure 9329 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1113900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3123 bp

ATGAGCTTCTCCGCGAAGCCTCAAACCCTAACCCTAATCTCATGCACTCCTCTCTCTTCTTCTTCCTCTTCCCATCGCCGATCGATTCGTAGAGACTTTCTCGGCTGCGGCCAGAGCCTCCGCCCGTTCGCCGGCGGTATCCGCTCCCACAGAGAACGCAGGAGTTTGGACGGAGGTCACCGCTCTCCTCCCTCCAAGTTTCTCATCAGAGCTTCTCTGGATTCGCATTCCCTCCTCGTCGTGGTCGCCGTCGTCACCTTCTCTGCTGCCTCCGTCGTCTACTTCAATCGCTCCGTCAAGCCCCGGAAGAATTTTGATGTTGGGGGCAGAGAGATTAGGGAGGTTAATGGTAAAGCTCCGATTGAAGAGAATAAGATTCTTGGTTTTGATGGTTTGAGCGGCAAAGTTGAAGAAATTGAGACGCCGGCGCCGACGCCGGTGCTGCAATTTCACAATTCAGGGCAGGAGTCGCTTTCTCCTTTGGTGTTCGAATCAACCGCGGTGTTTGAACCGCTTCGTTTCTCTGCAGAATTGGCACAGCTGCAGCAACAGGAACGGTCTGAATGTGTTGATTACGACCCAATTTCTGAGGAGTTCTCCAAATTAGTAGGTGAATCTAATTTCGGCGTGGCTTTGGAGCCTGATTCTGTGAGTGATGAGGAAACCGATGAAGGCGATGAAGAAGATGAAGTGGATAAAGTCAGTGAAGTGGGTGAAGCTACTGGATTTCCTTTGCTTCATAGAGAATCAGTGAGGGAAGAGCTTCACATGTTTTATGAGGGAAACAAGTCTGAGACAAAATCAGAAGAAAGCATGAATGGTAAAAAATTATCTTCGTTTTTACGAAACAGCACCGTAACAGGGGCCGGTTTACTGCCACAAGTTTCCGATCACACTACAGAGTCTATGGAAGGAAAGATAGATAGTGCTAGTTATAAAGGAGTTCACAGCAGAAAGGACTTGGGAAATGGCAGTGGATATGCAAGCGACAGAGAAATAAGAAATTCGGCTACCCAGATTCCTCACCCAAATGGTGTTCTTACAAATGACAGAGATATTCTATCAGAGCAATTAGGTGCCTACCATCGTTTGCTTAAGGATGGGAGGTTAGCCGACTCTCTAAAGTTGCTTGAAGATTTGCAAAGAAGGGGTTTGTTGGATATGAATAAGGTTTATCATGCAAGGTTTTTTGAAAACTGCAAATCGAAAAAGGCTGTTGATGAAGCTTTTCGTTTCATTGAACTGATTCCCAATCCAACATTGAGTACATACAATATGCTCATGACTGTCTGTGCAAGTTCTCAAGATTCAGAGGGAGCTTTCCAAGTTGTGAGACTTGCTCGGGAGGCTGGACTGAAGCCCGACTGCAAACTTTACACCACTCTGATATCAACATGCGGTAAAAGTGGAAAAGTGTACACGATGTTTGATGTCTTCCATGAGATGGTTAATGCTGGAGTAGAGCCAAACGTTCACACATATGGGGCACTGATTGATGGTTGTGGCAGAGCTGGGGAAGTGGCCAAGGCATTTGGTGCTTATGGGATAATGAGGTCTAAGAAGGTGAAGCCAGACCGAGTTGTATTCAATGCGCTTATTACTGCATGTGGTCAATCAGGAGCAGTTGATCGTGCCTTTGACGTTTTAGGAGAAATGCTGGCAGAAACACAACCTATAGAACCTGATCACACTACCGTCGGTGCTCTAATAAAGGCATGCGCAAATGCTGGTCAGGTTGATCGGGCACGAGAAGTATATAAGTTGGTCCATAAATATAAGATAAGGGGCTCCTCTGAGGTTTACACCATAGCTGTTAATTCTTGCAGCCAGACAGGTGATTGGGAATTTGCTTGCATTGTGTATAGTGACATGACTAGTAAAGGCGTGGTCCCTGATGAGATGTTTCTCAGTGCACTAATAGATGTTGCTGGGCATGCTGGAAAGCTAGATGCTGCCTTTGGTATCCTACACGAAGCTAGAAATCGGGGAATACAAGTTGGAACTGTATCTTACAGCTCATTGATGGGAGCCTGTAGCAATGCTAAAAACTGGCAGAAAGCACTGGACCTCTATGAGTATCTCAAGGCCACCAAAATTGAGCAATCTGTTTCAACAGTCAATGCTTTAATCACTGCCTTGTGTGACGGGGATCAACTACAGAAGGCTATGGAAGTTCTATCACAAATGAAGGGATTAGGATTGCGTCCAAACAGCATAACATACTCCATACTTATAGTGGCAAGTGAAAAAAAGGATGACCTAGGATCTGGACAAATGCTCCTTTCTCAAGCCGAAAAGGATGGTGTTGCTCCAAACCTTGTCATGTGTAGATGCATTATTGGCATGTGCTTACGGAGATCTGAGAAGGCATGTACACTTGGTGAACCTGCTTTGTCGGTTGATCGGCCTCAAGTTGATAGTGAATGGGCATCTTTGGCCTTAATGGTCTACCGCAAAACTATAGTAGCTGGTAATGTTCCCACCATTGAAGTTATATCACAAGTCTTGGGATGCTTGCAGCTCCCTTACGATGTATCATTCAAGAATAGGCTTATAGAGAATCTTGGTGTATCTGCCGACACATCAAGACCTTCAAACCTTTGTTCATTGATAGATGGGTTTGGTGAATATGACCCTCGTGCATTCTCATTACTGGAGGAAGCTGCTTCACTTGGAATTGTTCCATGTGTATCCTTCAAAGCAAGTCCTGTTGTTATTGACGCAAGGAAGTTGCAAATGCATACTGCGGAGGTTTACATCTTGACCGTTTTGAGAGGTCTCAGACATCGACTTGCTGCTGGTGCAAAGTTACCCAACATGACCATTTTACTGCCTGTAGAGAAGACACAAATTGTGTCTACCAAAGGGAAGACAATCAACATTGCCGGAAGGATTGGTCAGTCAGTTGCAGCATTATTAAGAAGGCTTGGGTTACCGTACCAAGGAAATGAATCACATGGAAAAATTAAAATCAGTGGGTTAGCCATGAAAAGATGGTTTCAACCAAAGCTTGCTTCTTTCACTGGAAAACCAGGAGAATTTGGCTCCTCGCAGTTACGGCTGGGGAAGGGAATCACACACCAGCAGCGGGACATTCGAACTGGCAATCTGTCCTTGGAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009507 GO:0009536 GO:0009892 GO:0009893 GO:0009894 GO:0009895 GO:0009987 GO:0010468 GO:0010604 GO:0010605 GO:0010608 GO:0010628 GO:0016070 GO:0016787 GO:0016788 GO:0019219 GO:0019222 GO:0031323 GO:0031324 GO:0031329 GO:0031330 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043487 GO:0043488 GO:0043489 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0045934 GO:0046483 GO:0048255 GO:0048518 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0060255 GO:0061013 GO:0065007 GO:0065008 GO:0071704 GO:0080090 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363 GO:1902369 GO:1902373 GO:1903311 GO:1903312

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2 PPR_3

Protein Analysis

1041

Amino Acids

113.49

Weight (kDa)

6.77

Isoelectric Point (pI)

42.41

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_long	PF17177	423 - 550	8.7e-12	Pentacotripeptide-repeat region of PRORP
PPR_2	PF13041	455 - 502	3.4e-12	PPR repeat family
TPR_24	PF23276	462 - 587	1e-12	Fungal tetratrico peptide repeats
PPR_3	PF13812	478 - 538	5.9e-12	Pentatricopeptide repeat domain
PPR_1	PF12854	486 - 511	2.6e-06	PPR repeat
PPR_2	PF13041	496 - 536	7.2e-06	PPR repeat family
PPR_long	PF17177	498 - 666	6.2e-17	Pentacotripeptide-repeat region of PRORP
PPR_2	PF13041	524 - 575	3e-07	PPR repeat family
TPR_24	PF23276	605 - 727	7.8e-09	Fungal tetratrico peptide repeats
PPR_3	PF13812	655 - 713	2.7e-07	Pentatricopeptide repeat domain
PPR_2	PF13041	667 - 715	2e-07	PPR repeat family
PPR_3	PF13812	694 - 746	5.6e-07	Pentatricopeptide repeat domain
PPR_2	PF13041	702 - 745	3.2e-11	PPR repeat family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0004157)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G34830
fragaria_vesca	FvH4_2g02240 FvH4_2g02240 FvH4_2g02240 FvH4_2g02240 FvH4_2g02240 FvH4_2g02240 FvH4_2g02240 FvH4_2g02240 FvH4_2g02240 FvH4_2g02240 FvH4_2g02240 FvH4_2g02240 FvH4_2g02240
malus_domestica	MD05G1113900.v1.1
prunus_persica	Prupe.8G158300_v2.0.a1 Prupe.8G158300_v2.0.a1 Prupe.8G158300_v2.0.a1
pyrus_communis	pycom05g10870 pycom10g10060
rosa_chinensis	RchiOBHm_Chr6g0245931
rosa_laevigata	RLG00000015285
rosa_multiflora	Rmu_co8211740.1_g000001 Rmu_sc0005570.1_g000001 Rmu_sc0018377.1_g000001 Rmu_sc0018378.1_g000004
rosa_roxburghii	Rroxscaffold_7G00215060
rosa_rugosa	Rorug05G0517500
rosa_samantha	Rh6AG031800 Rh6BG028200 Rh6CG028200 Rh6DG027500
rosa_wichuraiana	Rw6G002660

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	936
Acc16I	TGCGCA	1 cut(s)	1720
Acc36I	ACCTGC	1 cut(s)	2395
AccB1I	GGYRCC	2 cut(s)	427, 1076
AccB7I	CCANNNNNTGG	1 cut(s)	1444
AccBSI	CCGCTC	3 cut(s)	144, 183, 399
AccI	GTMKAC	3 cut(s)	279, 2448, 2858
AccII	CGCG	2 cut(s)	14, 500
AclI	AACGTT	1 cut(s)	1470
AclWI	GGATC	2 cut(s)	2157, 2272
AcoI	YGGCCR	2 cut(s)	109, 1521
AcsI	RAATTY	4 cut(s)	310, 1003, 1844, 3038
AcuI	CTGAAG	1 cut(s)	1376
AcyI	GRCGYC	3 cut(s)	422, 428, 434
AdeI	CACNNNGTG	1 cut(s)	2381
AfaI	GTAC	4 cut(s)	1267, 1418, 2374, 2939
AfiI	CCNNNNNNNGG	6 cut(s)	131, 241, 861, 1444, 2942, 3088
AflII	CTTAAG	1 cut(s)	1094
AflIII	ACRYGT	1 cut(s)	768
AhlI	ACTAGT	1 cut(s)	1874
AjnI	CCWGG	1 cut(s)	3031
AjuI	GAANNNNNNNTTGG	2 cut(s)	2998, 3030
Alw21I	GWGCWC	2 cut(s)	1705, 1915
Alw26I	GTCTC	5 cut(s)	87, 413, 788, 1327, 2783
Alw44I	GTGCAC	1 cut(s)	1911
AlwI	GGATC	2 cut(s)	2157, 2272
Ama87I	CYCGRG	1 cut(s)	1341
AoxI	GGCC	6 cut(s)	109, 864, 1521, 2085, 2405, 2436
ApaLI	GTGCAC	1 cut(s)	1911
ApoI	RAATTY	4 cut(s)	310, 1003, 1844, 3038
Asp700I	GAANNNNTTC	4 cut(s)	513, 762, 1227, 1245
AspA2I	CCTAGG	1 cut(s)	2260
AspLEI	GCGC	3 cut(s)	430, 1594, 1721
AspS9I	GGNCC	4 cut(s)	864, 1765, 1888, 2065
AsuC2I	CCSGG	1 cut(s)	304
AsuHPI	GGTGA	9 cut(s)	170, 247, 623, 725, 1016, 1576, 1847, 2393, 2636
AsuII	TTCGAA	2 cut(s)	489, 3097
AvaI	CYCGRG	1 cut(s)	1341
AvaII	GGWCC	3 cut(s)	1765, 1888, 2065
AvrII	CCTAGG	1 cut(s)	2260
BaeGI	GKGCMC	3 cut(s)	1489, 1747, 1915
BalI	TGGCCA	1 cut(s)	1523
BanI	GGYRCC	2 cut(s)	427, 1076
BanII	GRGCYC	1 cut(s)	1790
BauI	CACGAG	2 cut(s)	1746, 2637
BbsI	GAAGAC	3 cut(s)	1426, 2849, 2876
Bbv12I	GWGCWC	2 cut(s)	1705, 1915
BceAI	ACGGC	2 cut(s)	233, 3074
BcgI	CGANNNNNNTGC	2 cut(s)	421, 455
BciT130I	CCWGG	1 cut(s)	3033
BciVI	GTATCC	3 cut(s)	149, 1973, 2701
BclI	TGATCA	1 cut(s)	1684
BcnI	CCSGG	1 cut(s)	304
BcoDI	GTCTC	5 cut(s)	87, 413, 788, 1327, 2783
BcuI	ACTAGT	1 cut(s)	1874
BfaI	CTAG	5 cut(s)	930, 1875, 1946, 1977, 2261
BfmI	CTRYAG	8 cut(s)	525, 542, 897, 1674, 2032, 2156, 2460, 2835
BfoI	RGCGCY	1 cut(s)	431
BfrI	CTTAAG	1 cut(s)	1094
BfuAI	ACCTGC	1 cut(s)	2395
BfuI	GTATCC	3 cut(s)	149, 1973, 2701
BglII	AGATCT	1 cut(s)	2357
BlnI	CCTAGG	1 cut(s)	2260
Bme1390I	CCNGG	2 cut(s)	304, 3033
Bme18I	GGWCC	3 cut(s)	1765, 1888, 2065
BmeT110I	CYCGRG	1 cut(s)	1341
BmgT120I	GGNCC	4 cut(s)	864, 1765, 1888, 2065
BmiI	GGNNCC	8 cut(s)	429, 639, 865, 1078, 1789, 1890, 2029, 3046
BmrFI	CCNGG	2 cut(s)	304, 3033
BmsI	GCATC	4 cut(s)	1939, 2321, 2438, 2501
BoxI	GACNNNNGTC	1 cut(s)	174
BpiI	GAAGAC	3 cut(s)	1426, 2849, 2876
BplI	GAGNNNNNCTC	6 cut(s)	84, 116, 581, 613, 1324, 1356
BpmI	CTGGAG	2 cut(s)	1476, 2675
Bpu14I	TTCGAA	2 cut(s)	489, 3097
BpuEI	CTTGAG	4 cut(s)	1287, 2066, 2271, 2394
BpuMI	CCSGG	1 cut(s)	304
Bsa29I	ATCGAT	1 cut(s)	83
BsaHI	GRCGYC	3 cut(s)	422, 428, 434
BsaI	GGTCTC	1 cut(s)	2783
BsaJI	CCNNGG	6 cut(s)	302, 498, 1524, 2260, 2941, 3114
BsaXI	ACNNNNNCTCC	2 cut(s)	2262, 2292
Bsc4I	CCNNNNNNNGG	6 cut(s)	131, 241, 861, 1444, 2942, 3088
Bse118I	RCCGGY	4 cut(s)	131, 424, 436, 866
Bse1I	ACTGG	6 cut(s)	727, 2054, 2067, 2658, 3028, 3106
Bse3DI	GCAATG	2 cut(s)	2044, 2880
BseBI	CCWGG	1 cut(s)	3033
BseCI	ATCGAT	1 cut(s)	83
BseDI	CCNNGG	6 cut(s)	302, 498, 1524, 2260, 2941, 3114
BseGI	GGATG	4 cut(s)	1105, 2260, 2305, 2516
BseLI	CCNNNNNNNGG	6 cut(s)	131, 241, 861, 1444, 2942, 3088
BseMI	GCAATG	2 cut(s)	2044, 2880
BseMII	CTCAG	6 cut(s)	579, 783, 1785, 1921, 2352, 2794
BseNI	ACTGG	6 cut(s)	727, 2054, 2067, 2658, 3028, 3106
BseRI	GAGGAG	6 cut(s)	39, 177, 224, 605, 1780, 3037
BseSI	GKGCMC	3 cut(s)	1489, 1747, 1915
BseYI	CCCAGC	3 cut(s)	1511, 1928, 3061
Bsh1236I	CGCG	2 cut(s)	14, 500
Bsh1285I	CGRYCG	1 cut(s)	83
BshFI	GGCC	6 cut(s)	111, 866, 1523, 2087, 2407, 2438
BshNI	GGYRCC	2 cut(s)	427, 1076
BshVI	ATCGAT	1 cut(s)	83
BsiEI	CGRYCG	1 cut(s)	83
BsiHKAI	GWGCWC	2 cut(s)	1705, 1915
BsiHKCI	CYCGRG	1 cut(s)	1341
BsiSI	CCGG	6 cut(s)	132, 304, 425, 437, 867, 2886
BslFI	GGGAC	2 cut(s)	1874, 3104
BslI	CCNNNNNNNGG	6 cut(s)	131, 241, 861, 1444, 2942, 3088
BsmAI	GTCTC	5 cut(s)	87, 413, 788, 1327, 2783
BsmBI	CGTCTC	1 cut(s)	413
BsmFI	GGGAC	2 cut(s)	1874, 3104
BsmI	GAATGC	2 cut(s)	226, 2642
BsnI	GGCC	6 cut(s)	111, 866, 1523, 2087, 2407, 2438
Bso31I	GGTCTC	1 cut(s)	2783
BsoBI	CYCGRG	1 cut(s)	1341
Bsp119I	TTCGAA	2 cut(s)	489, 3097
Bsp1286I	GDGCHC	5 cut(s)	1489, 1705, 1747, 1790, 1915
Bsp1407I	TGTACA	2 cut(s)	1416, 2372
Bsp143I	GATC	9 cut(s)	80, 889, 1627, 1684, 1738, 2149, 2264, 2357, 2401
BspANI	GGCC	6 cut(s)	111, 866, 1523, 2087, 2407, 2438
BspCNI	CTCAG	6 cut(s)	580, 784, 1786, 1920, 2353, 2793
BspDI	ATCGAT	1 cut(s)	83
BspFNI	CGCG	2 cut(s)	14, 500
BspHI	TCATGA	1 cut(s)	1281
BspLI	GGNNCC	8 cut(s)	429, 639, 865, 1078, 1789, 1890, 2029, 3046
BspMAI	CTGCAG	2 cut(s)	529, 546
BspMI	ACCTGC	1 cut(s)	2395
BspPI	GGATC	2 cut(s)	2157, 2272
BspQI	GCTCTTC	1 cut(s)	753
BspT104I	TTCGAA	2 cut(s)	489, 3097
BspT107I	GGYRCC	2 cut(s)	427, 1076
BspTI	CTTAAG	1 cut(s)	1094
BspTNI	GGTCTC	1 cut(s)	2783
BsrBI	CCGCTC	3 cut(s)	144, 183, 399
BsrDI	GCAATG	2 cut(s)	2044, 2880
BsrFI	RCCGGY	4 cut(s)	131, 424, 436, 866
BsrGI	TGTACA	2 cut(s)	1416, 2372
BsrI	ACTGG	6 cut(s)	727, 2054, 2067, 2658, 3028, 3106
BssAI	RCCGGY	4 cut(s)	131, 424, 436, 866
BssECI	CCNNGG	6 cut(s)	302, 498, 1524, 2260, 2941, 3114
BssMI	GATC	9 cut(s)	80, 889, 1627, 1684, 1738, 2149, 2264, 2357, 2401
BssNI	GRCGYC	3 cut(s)	422, 428, 434
BssSI	CACGAG	2 cut(s)	1746, 2637
BssT1I	CCWWGG	4 cut(s)	1524, 2260, 2941, 3114
Bst2BI	CACGAG	2 cut(s)	1746, 2637
Bst2UI	CCWGG	1 cut(s)	3033
Bst4CI	ACNGT	8 cut(s)	558, 856, 1288, 1696, 2005, 2116, 2767, 2937
Bst6I	CTCTTC	4 cut(s)	61, 73, 360, 753
BstACI	GRCGYC	3 cut(s)	422, 428, 434
BstAFI	CTTAAG	1 cut(s)	1094
BstAPI	GCANNNNNTGC	1 cut(s)	1535
BstAUI	TGTACA	2 cut(s)	1416, 2372
BstBI	TTCGAA	2 cut(s)	489, 3097
BstC8I	GCNNGC	9 cut(s)	133, 426, 985, 1659, 1717, 1852, 1935, 2516, 3012
BstDEI	CTNAG	7 cut(s)	588, 792, 1557, 1794, 1907, 2361, 2780
BstDSI	CCRYGG	1 cut(s)	498
BstEII	GGTNACC	2 cut(s)	176, 2931
BstF5I	GGATG	4 cut(s)	1105, 2260, 2305, 2516
BstFNI	CGCG	2 cut(s)	14, 500
BstH2I	RGCGCY	1 cut(s)	431
BstHHI	GCGC	3 cut(s)	430, 1594, 1721
BstKTI	GATC	9 cut(s)	83, 892, 1630, 1687, 1741, 2152, 2267, 2360, 2404
BstMAI	GTCTC	5 cut(s)	87, 413, 788, 1327, 2783
BstMBI	GATC	9 cut(s)	80, 889, 1627, 1684, 1738, 2149, 2264, 2357, 2401
BstMCI	CGRYCG	1 cut(s)	83
BstNI	CCWGG	1 cut(s)	3033
BstNSI	RCATGY	7 cut(s)	772, 1398, 1608, 1719, 1937, 2347, 2373
BstPAI	GACNNNNGTC	1 cut(s)	174
BstPI	GGTNACC	2 cut(s)	176, 2931
BstSCI	CCNGG	2 cut(s)	302, 3031
BstSFI	CTRYAG	8 cut(s)	525, 542, 897, 1674, 2032, 2156, 2460, 2835
BstSLI	GKGCMC	3 cut(s)	1489, 1747, 1915
BstUI	CGCG	2 cut(s)	14, 500
BstV2I	GAAGAC	3 cut(s)	1426, 2849, 2876
BstX2I	RGATCY	2 cut(s)	2264, 2357
BstYI	RGATCY	2 cut(s)	2264, 2357
Bsu15I	ATCGAT	1 cut(s)	83
BsuI	GTATCC	3 cut(s)	149, 1973, 2701
BsuRI	GGCC	6 cut(s)	111, 866, 1523, 2087, 2407, 2438
BsuTUI	ATCGAT	1 cut(s)	83
BtgI	CCRYGG	1 cut(s)	498
BtgZI	GCGATG	2 cut(s)	59, 690
BtsCI	GGATG	4 cut(s)	1105, 2260, 2305, 2516
BtsI	GCAGTG	2 cut(s)	979, 2130
BtsIMutI	CAGTG	9 cut(s)	709, 756, 979, 1487, 1915, 2060, 2130, 2980, 3021
BveI	ACCTGC	1 cut(s)	2395
Cac8I	GCNNGC	9 cut(s)	133, 426, 985, 1659, 1717, 1852, 1935, 2516, 3012
CciI	TCATGA	1 cut(s)	1281
CfoI	GCGC	3 cut(s)	430, 1594, 1721
Cfr10I	RCCGGY	4 cut(s)	131, 424, 436, 866
Cfr13I	GGNCC	4 cut(s)	864, 1765, 1888, 2065
Cfr42I	CCGCGG	1 cut(s)	501
ClaI	ATCGAT	1 cut(s)	83
CseI	GACGC	4 cut(s)	430, 442, 2192, 2729
Csp6I	GTAC	4 cut(s)	1266, 1417, 2373, 2938
CspCI	CAANNNNNGTGG	2 cut(s)	2077, 2112
CviQI	GTAC	4 cut(s)	1266, 1417, 2373, 2938
DdeI	CTNAG	7 cut(s)	588, 792, 1557, 1794, 1907, 2361, 2780
DinI	GGCGCC	1 cut(s)	429
DpnI	GATC	9 cut(s)	82, 891, 1629, 1686, 1740, 2151, 2266, 2359, 2403
DpnII	GATC	9 cut(s)	80, 889, 1627, 1684, 1738, 2149, 2264, 2357, 2401
DraIII	CACNNNGTG	1 cut(s)	2381
EaeI	YGGCCR	2 cut(s)	109, 1521
Eam1104I	CTCTTC	4 cut(s)	61, 73, 360, 753
EarI	CTCTTC	4 cut(s)	61, 73, 360, 753
EciI	GGCGGA	1 cut(s)	110
Eco130I	CCWWGG	4 cut(s)	1524, 2260, 2941, 3114
Eco24I	GRGCYC	1 cut(s)	1790
Eco31I	GGTCTC	1 cut(s)	2783
Eco32I	GATATC	1 cut(s)	1389
Eco47I	GGWCC	3 cut(s)	1765, 1888, 2065
Eco57I	CTGAAG	1 cut(s)	1376
Eco88I	CYCGRG	1 cut(s)	1341
Eco91I	GGTNACC	2 cut(s)	176, 2931
EcoO65I	GGTNACC	2 cut(s)	176, 2931
EcoRII	CCWGG	1 cut(s)	3031
EcoRV	GATATC	1 cut(s)	1389
EcoT14I	CCWWGG	4 cut(s)	1524, 2260, 2941, 3114
EcoT22I	ATGCAT	2 cut(s)	2336, 2742
EcoT38I	GRGCYC	1 cut(s)	1790
EgeI	GGCGCC	1 cut(s)	429
EheI	GGCGCC	1 cut(s)	429
ErhI	CCWWGG	4 cut(s)	1524, 2260, 2941, 3114
Esp3I	CGTCTC	1 cut(s)	413
FaqI	GGGAC	2 cut(s)	1874, 3104
FauI	CCCGC	1 cut(s)	3081
FauNDI	CATATG	1 cut(s)	1480
FbaI	TGATCA	1 cut(s)	1684
FblI	GTMKAC	3 cut(s)	279, 2448, 2858
FokI	GGATG	4 cut(s)	1112, 2267, 2312, 2523
FriOI	GRGCYC	1 cut(s)	1790
FspBI	CTAG	5 cut(s)	930, 1875, 1946, 1977, 2261
FspI	TGCGCA	1 cut(s)	1720
GlaI	GCGC	3 cut(s)	429, 1593, 1720
GsaI	CCCAGC	3 cut(s)	1515, 1932, 3065
GsuI	CTGGAG	2 cut(s)	1476, 2675
HaeII	RGCGCY	1 cut(s)	431
HaeIII	GGCC	6 cut(s)	111, 866, 1523, 2087, 2407, 2438
HapII	CCGG	6 cut(s)	132, 304, 425, 437, 867, 2886
HgaI	GACGC	4 cut(s)	430, 442, 2192, 2729
HhaI	GCGC	3 cut(s)	430, 1594, 1721
Hin1I	GRCGYC	3 cut(s)	422, 428, 434
Hin6I	GCGC	3 cut(s)	428, 1592, 1719
HinP1I	GCGC	3 cut(s)	428, 1592, 1719
HindIII	AAGCTT	2 cut(s)	1224, 3008
HpaII	CCGG	6 cut(s)	132, 304, 425, 437, 867, 2886
HphI	GGTGA	9 cut(s)	170, 247, 623, 725, 1016, 1576, 1847, 2393, 2636
Hpy188III	TCNNGA	9 cut(s)	218, 1282, 1304, 1343, 1617, 2268, 2542, 2586, 2761
Hpy99I	CGWCG	5 cut(s)	242, 254, 278, 436, 1700
HpyCH4III	ACNGT	8 cut(s)	558, 856, 1288, 1696, 2005, 2116, 2767, 2937
HpyCH4IV	ACGT	2 cut(s)	1470, 1641
HpyF3I	CTNAG	7 cut(s)	588, 792, 1557, 1794, 1907, 2361, 2780
HpySE526I	ACGT	2 cut(s)	1470, 1641
Hsp92I	GRCGYC	3 cut(s)	422, 428, 434
HspAI	GCGC	3 cut(s)	428, 1592, 1719
KasI	GGCGCC	1 cut(s)	427
KroI	GCCGGC	2 cut(s)	131, 424
KroNI	GCCGGC	2 cut(s)	133, 426
Ksp22I	TGATCA	1 cut(s)	1684
KspI	CCGCGG	1 cut(s)	501
Kzo9I	GATC	9 cut(s)	80, 889, 1627, 1684, 1738, 2149, 2264, 2357, 2401
LguI	GCTCTTC	1 cut(s)	753
LweI	GCATC	4 cut(s)	1939, 2321, 2438, 2501
MaeI	CTAG	5 cut(s)	930, 1875, 1946, 1977, 2261
MaeII	ACGT	2 cut(s)	1470, 1641
MaeIII	GTNAC	8 cut(s)	176, 253, 856, 1865, 2141, 2808, 2931, 3054
MalI	GATC	9 cut(s)	82, 891, 1629, 1686, 1740, 2151, 2266, 2359, 2403
MbiI	CCGCTC	3 cut(s)	144, 183, 399
MboI	GATC	9 cut(s)	80, 889, 1627, 1684, 1738, 2149, 2264, 2357, 2401
MflI	RGATCY	2 cut(s)	2264, 2357
MhlI	GDGCHC	5 cut(s)	1489, 1705, 1747, 1790, 1915
MlsI	TGGCCA	1 cut(s)	1523
MluNI	TGGCCA	1 cut(s)	1523
Mly113I	GGCGCC	1 cut(s)	428
MlyI	GAGTC	3 cut(s)	476, 911, 1109
MmeI	TCCRAC	3 cut(s)	1137, 1280, 1978
Mox20I	TGGCCA	1 cut(s)	1523
Mph1103I	ATGCAT	2 cut(s)	2336, 2742
MreI	CGCCGGCG	2 cut(s)	131, 424
MroNI	GCCGGC	2 cut(s)	131, 424
MroXI	GAANNNNTTC	4 cut(s)	513, 762, 1227, 1245
MscI	TGGCCA	1 cut(s)	1523
MseI	TTAA	8 cut(s)	345, 1095, 1449, 1815, 2126, 2441, 2918, 2967
MslI	CAYNNNNRTG	2 cut(s)	1029, 1479
Msp20I	TGGCCA	1 cut(s)	1523
MspA1I	CMGCKG	3 cut(s)	500, 541, 3088
MspCI	CTTAAG	1 cut(s)	1094
MspI	CCGG	6 cut(s)	132, 304, 425, 437, 867, 2886
MspR9I	CCNGG	2 cut(s)	304, 3033
Mva1269I	GAATGC	2 cut(s)	226, 2642
MvaI	CCWGG	1 cut(s)	3033
MvnI	CGCG	2 cut(s)	14, 500
NaeI	GCCGGC	2 cut(s)	133, 426
NarI	GGCGCC	1 cut(s)	428
NciI	CCSGG	1 cut(s)	304
NdeI	CATATG	1 cut(s)	1480
NdeII	GATC	9 cut(s)	80, 889, 1627, 1684, 1738, 2149, 2264, 2357, 2401
NgoMIV	GCCGGC	2 cut(s)	131, 424
NlaIV	GGNNCC	8 cut(s)	429, 639, 865, 1078, 1789, 1890, 2029, 3046
NmeAIII	GCCGAG	1 cut(s)	81
NmuCI	GTSAC	4 cut(s)	176, 253, 1865, 2141
NsbI	TGCGCA	1 cut(s)	1720
NsiI	ATGCAT	2 cut(s)	2336, 2742
NspI	RCATGY	7 cut(s)	772, 1398, 1608, 1719, 1937, 2347, 2373
NspV	TTCGAA	2 cut(s)	489, 3097
PaeI	GCATGC	2 cut(s)	1719, 1937
PagI	TCATGA	1 cut(s)	1281
PciI	ACATGT	1 cut(s)	768
PciSI	GCTCTTC	1 cut(s)	753
PcsI	WCGNNNNNNNCGW	2 cut(s)	246, 428
PctI	GAATGC	2 cut(s)	226, 2642
PdiI	GCCGGC	2 cut(s)	133, 426
PdmI	GAANNNNTTC	4 cut(s)	513, 762, 1227, 1245
PflMI	CCANNNNNTGG	1 cut(s)	1444
Ple19I	CGATCG	1 cut(s)	83
PleI	GAGTC	3 cut(s)	475, 910, 1109
PluTI	GGCGCC	1 cut(s)	431
PpsI	GAGTC	3 cut(s)	475, 910, 1109
PscI	ACATGT	1 cut(s)	768
PshAI	GACNNNNGTC	1 cut(s)	174
PsiI	TTATAA	1 cut(s)	936
Psp1406I	AACGTT	1 cut(s)	1470
Psp6I	CCWGG	1 cut(s)	3031
PspEI	GGTNACC	2 cut(s)	176, 2931
PspFI	CCCAGC	3 cut(s)	1511, 1928, 3061
PspGI	CCWGG	1 cut(s)	3031
PspN4I	GGNNCC	8 cut(s)	429, 639, 865, 1078, 1789, 1890, 2029, 3046
PspPI	GGNCC	4 cut(s)	864, 1765, 1888, 2065
PstI	CTGCAG	2 cut(s)	529, 546
PsuI	RGATCY	2 cut(s)	2264, 2357
PvuI	CGATCG	1 cut(s)	83
PvuII	CAGCTG	1 cut(s)	541
RsaI	GTAC	4 cut(s)	1267, 1418, 2374, 2939
RsaNI	GTAC	4 cut(s)	1266, 1417, 2373, 2938
RseI	CAYNNNNRTG	2 cut(s)	1029, 1479
SacII	CCGCGG	1 cut(s)	501
SapI	GCTCTTC	1 cut(s)	753
SaqAI	TTAA	8 cut(s)	345, 1095, 1449, 1815, 2126, 2441, 2918, 2967
Sau3AI	GATC	9 cut(s)	80, 889, 1627, 1684, 1738, 2149, 2264, 2357, 2401
Sau96I	GGNCC	4 cut(s)	864, 1765, 1888, 2065
SchI	GAGTC	3 cut(s)	476, 911, 1109
ScrFI	CCNGG	2 cut(s)	304, 3033
SduI	GDGCHC	5 cut(s)	1489, 1705, 1747, 1790, 1915
SfaNI	GCATC	4 cut(s)	1939, 2321, 2438, 2501
SfcI	CTRYAG	8 cut(s)	525, 542, 897, 1674, 2032, 2156, 2460, 2835
SfoI	GGCGCC	1 cut(s)	429
Sfr303I	CCGCGG	1 cut(s)	501
SfuI	TTCGAA	2 cut(s)	489, 3097
SgrAI	CRCCGGYG	3 cut(s)	131, 424, 436
SgrBI	CCGCGG	1 cut(s)	501
SinI	GGWCC	3 cut(s)	1765, 1888, 2065
SmiMI	CAYNNNNRTG	2 cut(s)	1029, 1479
SmlI	CTYRAG	5 cut(s)	1094, 1302, 2081, 2286, 2409
SmoI	CTYRAG	5 cut(s)	1094, 1302, 2081, 2286, 2409
SpeI	ACTAGT	1 cut(s)	1874
SphI	GCATGC	2 cut(s)	1719, 1937
SspDI	GGCGCC	1 cut(s)	427
SspMI	CTAG	5 cut(s)	930, 1875, 1946, 1977, 2261
StyD4I	CCNGG	2 cut(s)	302, 3031
StyI	CCWWGG	4 cut(s)	1524, 2260, 2941, 3114
TaaI	ACNGT	8 cut(s)	558, 856, 1288, 1696, 2005, 2116, 2767, 2937
TaiI	ACGT	2 cut(s)	1473, 1644
TaqI	TCGA	5 cut(s)	83, 489, 1205, 2788, 3097
TaqII	GACCGA	1 cut(s)	1590
TatI	WGTACW	3 cut(s)	1265, 1416, 2372
TauI	GCSGC	2 cut(s)	111, 402
Tru1I	TTAA	8 cut(s)	345, 1095, 1449, 1815, 2126, 2441, 2918, 2967
Tru9I	TTAA	8 cut(s)	345, 1095, 1449, 1815, 2126, 2441, 2918, 2967
TscAI	CASTG	9 cut(s)	709, 756, 979, 1494, 1915, 2067, 2137, 2980, 3028
TseFI	GTSAC	4 cut(s)	176, 253, 1865, 2141
Tsp45I	GTSAC	4 cut(s)	176, 253, 1865, 2141
TspGWI	ACGGA	4 cut(s)	186, 262, 283, 2368
TspRI	CASTG	9 cut(s)	709, 756, 979, 1494, 1915, 2067, 2137, 2980, 3028
Van91I	CCANNNNNTGG	1 cut(s)	1444
Vha464I	CTTAAG	1 cut(s)	1094
VneI	GTGCAC	1 cut(s)	1911
VpaK11BI	GGWCC	3 cut(s)	1765, 1888, 2065
XapI	RAATTY	4 cut(s)	310, 1003, 1844, 3038
XceI	RCATGY	7 cut(s)	772, 1398, 1608, 1719, 1937, 2347, 2373
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2233
XmaJI	CCTAGG	1 cut(s)	2260
XmiI	GTMKAC	3 cut(s)	279, 2448, 2858
XmnI	GAANNNNTTC	4 cut(s)	513, 762, 1227, 1245
XspI	CTAG	5 cut(s)	930, 1875, 1946, 1977, 2261
Zsp2I	ATGCAT	2 cut(s)	2336, 2742

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.