Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr6C

Orthogroup ID

OG0004157

Physical Location & Seq

Genomic Coordinates Forward (+)

2486533 .. 2494712

Gene Structure 8180 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh6CG028200.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3108 bp

ATGAGCTTCTCTGCAAAGCCTCAGCCACTCGCCCTAATCTCATGCACGCCTCACTCCCGCTCCCTCCGCCGCGACTTCCTCGGATGCGCCCACAGTCTCCGCCCACTCGCCGGCGGCCTCAGGTCTCTGCGAAAGAACCGGAGCCTCGCCGGCCCCGGCGGACGTCAGAATCCGCCGCCTCGGTTGTTGATCAAAGCGTCGCTTGGTTCTCACTCGATTCTGGTGTTCGTCGCGGTGGTGACGTTCTCGGCGGTTTCTATTGTGTACTTCAATCGGTGGGTGAAGCCGAAGAAGAGAGAGGTTAGTGGTGAAGTTGAAGTTCCGGTGGAAGATCAGAGTATTGATTTTGATGCTTTGAAAGATGAGATTAGAGAGGCGAGGGAGGAGGAAGAGGCTTCTTCTTCTTCTCCGGTTCTGCAGTTTCACAATTCGTCTGTGATTTCTGCTCAGGAGTCTCAGTCTCTTTCGCCTTTGGTGTTTGAATCCACGGCTGTGCTTCAGCCGCTTCATTTTCCGACGGAAGTCACTGAGTTTGATAAACTTGAGCCTCTTTTCGAGTTGCCTAATCTGATGGGCGACTCTGATTTTGGCTCTGTGACTGTGAGTGATGAGGAAGTCGTCACTGAGTCACCTTCTGTGTCTGGAAGTGACGAGGAGAGCAGTGAGGTTGGGGAAGCTAATGGCTTTCGATTACTTAATGGAGAATCGGACAGAGAAGAGATTCACATGTTCTATGAGGCCAGCAAGTCTGAGATGAAGTTGGATGATGATAAGTTTTCTTCGTTTCTGAGAAATGGCACCCTCACAAAATCAGATTCGTTTCGACAAGTTTCGCATCACAGTACTACAGAGAATGCAGAAAGCAAGATACCTAATCACAAGGAAGGCCATGTCCGCAGCAGAGAAGACTTGGGAAACGGCAATGGATATAATGGACATGTAGCAGATAAAGAACTAAGACATTTGCCCAAAAAGAACCATAAGACTGTGCCCCAACCGAATGGAATACATACAAGCGACGCACATTATGTTTCCGAGCAATTAAGTGCTTACCATCGTTTGCTGAAGGATGGGAGGTTATCCGACTCTTTAAGATTGCTTGAAGATTTGGAGAAAAAGGATTTGTTGGATATGAATAAGGTTTATCATGGAAGGTTTTTTGAAACCTGCAAAAAGAAAAAGGCTGTTGATCAAGCTTTTCGTTTCATCAAACTGATTCCAAATCCAACAATGAGTACATATAATATGCTTATGTCTGTCTGTGCCAGTGCTCAAGATTCAGAGGGTGCTTTCAATGTTCTGGGACTTGTCCGGGAGGCTGGGCTGAGAGTTGATTGCAAACTTTACACTACTCTGATATCAACATGTGCGAAAAGTGGAAAAGTCTACACTATGTTTGATGTCTTCCATGAGATGGTTAGTGCTGGAGTAGAACCAAATGTTCACACATATGGGGCACTGATTGATGGTTGTGGCAGAGCTGGGGAAGTGGCAAAGGCATTTGGTGCTTATGGGATAATGAGGTCTAAGAAAGTGAAGCCAGATCGAGTTGTATTCAATGCGCTTATTACTGCGTGTGGTCAATCAGGAGCAGTTGACCGTGCTTTTGATGTCTTAGAAGAAATGAAGGCAGAGACACAGCCTATAGAACCTGATCACACAACTGTTGGTGCACTAATAAAAGCATGCGCAAATGCTGGTCAGGTTGAAAGGGCACGAGAAGTGTATAAGATGATTCATAAGTACAAGATTAAGGGCAGTTCTGAGGTTTACACCATAGCTGTTAATTGTTGCAGCCTGACTGGTGATTGGGACTTTGCTTGCACTGTATATGATGACATGACCAGGAATGGTGTGGTCCCTGATGAGATGTTCCTCAGTGCATTAATAGATGTTGCTGGGCATGCTGGAAAGCTAGATGCTGCCTTTGAAATCATACAGGAAGCCAGAAATCGAGGAATACAAGTTGGAACTGTATCATATAGCTCATTGATGGGCGCATGTAGCAATGCCAAAAATTGGCAGAAAGCACTGGGGCTCTATGAGGATCTTAAGTCCGCTAAAATTGAGCAGACTGTTTCAACTGTCAATGCTTTGATTACTGCCCTATGTGATGGGGATCAACTACAGAAGGCTATGGAAGTTCTGTCGGAAATGAAGAGTATAGGACTGCGTCCAAACAGTATAACATACTCCATACTTGTAGTGGCAAGTGAAAAGAAGGATGATCTAGAAGCTGGCCTGATGCTCCTTTCTCAAGCCGAAAAGGATAAAGTTGTTCCAAACCTTGTCATGTGTAGATGCATTATAGGCATGTGCTTACGGAGATCTGAGAAGGCATGTACACTGGGTGAACCTGTCTTGCCGCTTGACTCAGGCCGACCGCAAGTTGATAGTAAATGGGCATCAGTGGCCTTAATGGTCTATCGGAAAACTATTGTAGCTGGTGCTACTCCTACCATTGAAATTATATCACAAGTTTTGGGATGCTTGCAGCTCCCTTATGATGCATCATTTAAGAAGAGAGTTATTGAGAATCTTGGTGTAACTGCCGACACTTCAAGACCTTCAAAACTCTGTTCATTGATAGATGGGTTTGGTGAATATGACCCTCGTGCGTTTTCATTACTTGAGGAAGCTGCTTCACTTGGAATTGTTCCATGTGTATCCTTCAAAGTTAATCCCGTTGTTGTTGATGCAAAGAAGTTGCAACTTCATACTGCTGAGGTCTACATCTTGACTGTTCTGAGAGGTCTCAAACATCGGCTTGCTGCTGGTTCCAAGTTGCCCAACATGACCATTTTACTGCCTGCAGAAAAGACGCAAATTTTGTCCCCCAAGGGGAAGATGAAGACAATCTACCTTTCAGGAAGGGTTGGCCAGTCAGTTGCAGCATTATTGAGAAGGCTTGGGCTAGATTACCAAGGAAATGAATCTCGTGGAAAGATAAGAATCAGTGGGTTAACCATGAAAAGATGGTTTCAGCCAAAGCTTGCTTCACCATTCACTGGGAAACCGGAAGAAATAGGCTTGTCCCAGTTACGGCTGGGGAAAGGAATCACGCATCAGCAGCGCAGTATTCGAACCGGAAATTTTTCCTTGGATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009507 GO:0009536 GO:0009892 GO:0009893 GO:0009894 GO:0009895 GO:0009987 GO:0010468 GO:0010604 GO:0010605 GO:0010608 GO:0010628 GO:0016070 GO:0016787 GO:0016788 GO:0019219 GO:0019222 GO:0031323 GO:0031324 GO:0031329 GO:0031330 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043487 GO:0043488 GO:0043489 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0045934 GO:0046483 GO:0048255 GO:0048518 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0060255 GO:0061013 GO:0065007 GO:0065008 GO:0071704 GO:0080090 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363 GO:1902369 GO:1902373 GO:1903311 GO:1903312

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2 PPR_3

Protein Analysis

1035

Amino Acids

113.66

Weight (kDa)

7.74

Isoelectric Point (pI)

44.54

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_long	PF17177	412 - 583	5.9e-18	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	439 - 492	1.7e-08	Pentatricopeptide repeat domain
PPR_2	PF13041	448 - 492	3.9e-12	PPR repeat family
TPR_24	PF23276	452 - 578	6.1e-15	Fungal tetratrico peptide repeats
PPR_3	PF13812	468 - 528	1.5e-11	Pentatricopeptide repeat domain
PPR_1	PF12854	476 - 501	2.6e-06	PPR repeat
PPR_2	PF13041	486 - 526	1e-05	PPR repeat family
PPR_1	PF12854	511 - 543	8.9e-07	PPR repeat
PPR_2	PF13041	514 - 565	8e-09	PPR repeat family
TPR_24	PF23276	572 - 683	4.2e-06	Fungal tetratrico peptide repeats
PPR_long	PF17177	589 - 741	8.9e-13	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	645 - 703	1.4e-06	Pentatricopeptide repeat domain
PPR_2	PF13041	657 - 705	8.7e-08	PPR repeat family
PPR_3	PF13812	681 - 735	5.2e-07	Pentatricopeptide repeat domain
PPR_2	PF13041	692 - 735	4.8e-11	PPR repeat family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0004157)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G34830
fragaria_vesca	FvH4_2g02240 FvH4_2g02240 FvH4_2g02240 FvH4_2g02240 FvH4_2g02240 FvH4_2g02240 FvH4_2g02240 FvH4_2g02240 FvH4_2g02240 FvH4_2g02240 FvH4_2g02240 FvH4_2g02240 FvH4_2g02240
malus_domestica	MD05G1113900.v1.1
prunus_persica	Prupe.8G158300_v2.0.a1 Prupe.8G158300_v2.0.a1 Prupe.8G158300_v2.0.a1
pyrus_communis	pycom05g10870 pycom10g10060
rosa_chinensis	RchiOBHm_Chr6g0245931
rosa_laevigata	RLG00000015285
rosa_multiflora	Rmu_co8211740.1_g000001 Rmu_sc0005570.1_g000001 Rmu_sc0018377.1_g000001 Rmu_sc0018378.1_g000004
rosa_roxburghii	Rroxscaffold_7G00215060
rosa_rugosa	Rorug05G0517500
rosa_samantha	Rh6AG031800 Rh6BG028200 Rh6CG028200 Rh6DG027500
rosa_wichuraiana	Rw6G002660

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	166
Acc16I	TGCGCA	1 cut(s)	1690
Acc36I	ACCTGC	1 cut(s)	1175
AccB1I	GGYRCC	1 cut(s)	797
AccB7I	CCANNNNNTGG	3 cut(s)	1414, 2019, 3008
AccBSI	CCGCTC	1 cut(s)	60
AccI	GTMKAC	2 cut(s)	1386, 2730
AccII	CGCG	2 cut(s)	72, 233
AclWI	GGATC	2 cut(s)	2055, 2127
AcoI	YGGCCR	1 cut(s)	2878
AcsI	RAATTY	2 cut(s)	2826, 3091
AcuI	CTGAAG	2 cut(s)	482, 1085
AcyI	GRCGYC	1 cut(s)	163
AdeI	CACNNNGTG	1 cut(s)	2351
AfaI	GTAC	5 cut(s)	266, 844, 1237, 1745, 2344
AfiI	CCNNNNNNNGG	6 cut(s)	110, 1414, 2019, 2841, 3008, 3042
AflII	CTTAAG	1 cut(s)	2051
AflIII	ACRYGT	3 cut(s)	726, 937, 1364
AjnI	CCWGG	1 cut(s)	1844
AjuI	GAANNNNNNNTTGG	2 cut(s)	2980, 3012
AleI	CACNNNNGTG	1 cut(s)	491
Alw21I	GWGCWC	2 cut(s)	1273, 1675
Alw26I	GTCTC	6 cut(s)	101, 129, 459, 465, 1628, 2759
Alw44I	GTGCAC	1 cut(s)	1671
AlwI	GGATC	2 cut(s)	2055, 2127
AoxI	GGCC	8 cut(s)	115, 151, 738, 886, 2239, 2377, 2412, 2878
ApaLI	GTGCAC	1 cut(s)	1671
ApeKI	GCWGC	8 cut(s)	897, 1794, 1922, 2494, 2639, 2771, 2891, 3070
ApoI	RAATTY	2 cut(s)	2826, 3091
ArsI	GACNNNNNNTTYG	6 cut(s)	569, 601, 1589, 1621, 2812, 2844
AseI	ATTAAT	1 cut(s)	1886
Asp700I	GAANNNNTTC	2 cut(s)	720, 3094
AspLEI	GCGC	5 cut(s)	89, 1564, 1691, 2000, 3075
AspS9I	GGNCC	2 cut(s)	152, 1858
AsuC2I	CCSGG	2 cut(s)	156, 1313
AsuHPI	GGTGA	8 cut(s)	250, 292, 320, 621, 1817, 2363, 2612, 2991
AsuII	TTCGAA	1 cut(s)	3082
AvaII	GGWCC	1 cut(s)	1858
AxyI	CCTNAGG	1 cut(s)	119
BaeGI	GKGCMC	4 cut(s)	993, 1459, 1675, 1717
BalI	TGGCCA	1 cut(s)	2880
BanI	GGYRCC	1 cut(s)	797
BanII	GRGCYC	1 cut(s)	2040
BarI	GAAGNNNNNNTAC	2 cut(s)	2843, 2875
BauI	CACGAG	3 cut(s)	1716, 2613, 2937
BbsI	GAAGAC	3 cut(s)	912, 1396, 2858
Bbv12I	GWGCWC	2 cut(s)	1273, 1675
BbvCI	CCTCAGC	2 cut(s)	21, 2724
BbvI	GCAGC	8 cut(s)	909, 1806, 1909, 2506, 2626, 2758, 2903, 3082
BccI	CCATC	9 cut(s)	565, 1062, 1064, 1408, 1460, 1987, 2108, 2585, 2970
BceAI	ACGGC	3 cut(s)	504, 934, 3059
BciT130I	CCWGG	1 cut(s)	1846
BciVI	GTATCC	1 cut(s)	2677
BclI	TGATCA	3 cut(s)	189, 1189, 1654
BcnI	CCSGG	2 cut(s)	156, 1313
BcoDI	GTCTC	6 cut(s)	101, 129, 459, 465, 1628, 2759
BfaI	CTAG	3 cut(s)	1916, 2231, 2915
BfmI	CTRYAG	5 cut(s)	416, 846, 1644, 2126, 2811
BfrI	CTTAAG	1 cut(s)	2051
BfuAI	ACCTGC	1 cut(s)	1175
BfuI	GTATCC	1 cut(s)	2677
BglI	GCCNNNNNGGC	1 cut(s)	150
BglII	AGATCT	1 cut(s)	2327
BmcAI	AGTACT	1 cut(s)	844
Bme1390I	CCNGG	3 cut(s)	156, 1313, 1846
Bme18I	GGWCC	1 cut(s)	1858
BmgT120I	GGNCC	2 cut(s)	152, 1858
BmiI	GGNNCC	5 cut(s)	143, 154, 799, 1860, 2779
BmrFI	CCNGG	3 cut(s)	156, 1313, 1846
BmrI	ACTGGG	4 cut(s)	2042, 2357, 3018, 3031
BmuI	ACTGGG	4 cut(s)	2042, 2357, 3018, 3031
BoxI	GACNNNNGTC	1 cut(s)	521
BpiI	GAAGAC	3 cut(s)	912, 1396, 2858
BpmI	CTGGAG	1 cut(s)	1446
Bpu10I	CCTNAGC	3 cut(s)	21, 447, 2724
Bpu14I	TTCGAA	1 cut(s)	3082
BpuEI	CTTGAG	4 cut(s)	563, 1257, 2241, 2651
BpuMI	CCSGG	2 cut(s)	156, 1313
BsaBI	GATNNNNATC	2 cut(s)	2118, 2951
BsaHI	GRCGYC	1 cut(s)	163
BsaI	GGTCTC	2 cut(s)	129, 2759
BsaJI	CCNNGG	7 cut(s)	79, 154, 179, 486, 2838, 2923, 3099
BsaWI	WCCGGW	5 cut(s)	138, 322, 409, 3016, 3086
BsaXI	ACNNNNNCTCC	4 cut(s)	44, 74, 443, 473
Bsc4I	CCNNNNNNNGG	6 cut(s)	110, 1414, 2019, 2841, 3008, 3042
Bse118I	RCCGGY	2 cut(s)	110, 149
Bse1I	ACTGG	7 cut(s)	1266, 1807, 2037, 2352, 2881, 3013, 3037
Bse21I	CCTNAGG	1 cut(s)	119
Bse3DI	GCAATG	2 cut(s)	928, 2014
Bse8I	GATNNNNATC	2 cut(s)	2118, 2951
BseBI	CCWGG	1 cut(s)	1846
BseDI	CCNNGG	7 cut(s)	79, 154, 179, 486, 2838, 2923, 3099
BseGI	GGATG	5 cut(s)	89, 769, 1075, 2230, 2492
BseJI	GATNNNNATC	2 cut(s)	2118, 2951
BseLI	CCNNNNNNNGG	6 cut(s)	110, 1414, 2019, 2841, 3008, 3042
BseMI	GCAATG	2 cut(s)	928, 2014
BseNI	ACTGG	7 cut(s)	1266, 1807, 2037, 2352, 2881, 3013, 3037
BseRI	GAGGAG	2 cut(s)	398, 668
BseSI	GKGCMC	4 cut(s)	993, 1459, 1675, 1717
BseXI	GCAGC	8 cut(s)	909, 1806, 1909, 2506, 2626, 2758, 2903, 3082
BseYI	CCCAGC	4 cut(s)	1319, 1481, 1898, 3046
Bsh1236I	CGCG	2 cut(s)	72, 233
Bsh1285I	CGRYCG	1 cut(s)	2384
BshFI	GGCC	8 cut(s)	117, 153, 740, 888, 2241, 2379, 2414, 2880
BshNI	GGYRCC	1 cut(s)	797
BsiEI	CGRYCG	1 cut(s)	2384
BsiHKAI	GWGCWC	2 cut(s)	1273, 1675
BsiSI	CCGG	9 cut(s)	111, 139, 150, 156, 323, 410, 1312, 3017, 3087
BslFI	GGGAC	5 cut(s)	1317, 1826, 1844, 2818, 3019
BslI	CCNNNNNNNGG	6 cut(s)	110, 1414, 2019, 2841, 3008, 3042
BsmAI	GTCTC	6 cut(s)	101, 129, 459, 465, 1628, 2759
BsmFI	GGGAC	5 cut(s)	1317, 1826, 1844, 2818, 3019
BsmI	GAATGC	1 cut(s)	859
BsnI	GGCC	8 cut(s)	117, 153, 740, 888, 2241, 2379, 2414, 2880
Bso31I	GGTCTC	2 cut(s)	129, 2759
Bsp119I	TTCGAA	1 cut(s)	3082
Bsp1286I	GDGCHC	6 cut(s)	993, 1273, 1459, 1675, 1717, 2040
Bsp1407I	TGTACA	1 cut(s)	2342
Bsp143I	GATC	9 cut(s)	189, 331, 1189, 1543, 1654, 2047, 2119, 2227, 2327
BspANI	GGCC	8 cut(s)	117, 153, 740, 888, 2241, 2379, 2414, 2880
BspFNI	CGCG	2 cut(s)	72, 233
BspLI	GGNNCC	5 cut(s)	143, 154, 799, 1860, 2779
BspMAI	CTGCAG	2 cut(s)	420, 2815
BspMI	ACCTGC	1 cut(s)	1175
BspPI	GGATC	2 cut(s)	2055, 2127
BspT104I	TTCGAA	1 cut(s)	3082
BspT107I	GGYRCC	1 cut(s)	797
BspTI	CTTAAG	1 cut(s)	2051
BspTNI	GGTCTC	2 cut(s)	129, 2759
BsrBI	CCGCTC	1 cut(s)	60
BsrDI	GCAATG	2 cut(s)	928, 2014
BsrFI	RCCGGY	2 cut(s)	110, 149
BsrGI	TGTACA	1 cut(s)	2342
BsrI	ACTGG	7 cut(s)	1266, 1807, 2037, 2352, 2881, 3013, 3037
BssAI	RCCGGY	2 cut(s)	110, 149
BssECI	CCNNGG	7 cut(s)	79, 154, 179, 486, 2838, 2923, 3099
BssMI	GATC	9 cut(s)	189, 331, 1189, 1543, 1654, 2047, 2119, 2227, 2327
BssNI	GRCGYC	1 cut(s)	163
BssSI	CACGAG	3 cut(s)	1716, 2613, 2937
BssT1I	CCWWGG	3 cut(s)	2838, 2923, 3099
Bst2BI	CACGAG	3 cut(s)	1716, 2613, 2937
Bst2UI	CCWGG	1 cut(s)	1846
Bst6I	CTCTTC	5 cut(s)	287, 384, 711, 2153, 2516
BstACI	GRCGYC	1 cut(s)	163
BstAFI	CTTAAG	1 cut(s)	2051
BstAPI	GCANNNNNTGC	1 cut(s)	1505
BstAUI	TGTACA	1 cut(s)	2342
BstBI	TTCGAA	1 cut(s)	3082
BstDSI	CCRYGG	1 cut(s)	486
BstF5I	GGATG	5 cut(s)	89, 769, 1075, 2230, 2492
BstFNI	CGCG	2 cut(s)	72, 233
BstHHI	GCGC	5 cut(s)	89, 1564, 1691, 2000, 3075
BstKTI	GATC	9 cut(s)	192, 334, 1192, 1546, 1657, 2050, 2122, 2230, 2330
BstMAI	GTCTC	6 cut(s)	101, 129, 459, 465, 1628, 2759
BstMBI	GATC	9 cut(s)	189, 331, 1189, 1543, 1654, 2047, 2119, 2227, 2327
BstMCI	CGRYCG	1 cut(s)	2384
BstNI	CCWGG	1 cut(s)	1846
BstNSI	RCATGY	8 cut(s)	730, 941, 1368, 1689, 1907, 2004, 2317, 2343
BstPAI	GACNNNNGTC	1 cut(s)	521
BstSCI	CCNGG	3 cut(s)	154, 1311, 1844
BstSFI	CTRYAG	5 cut(s)	416, 846, 1644, 2126, 2811
BstSLI	GKGCMC	4 cut(s)	993, 1459, 1675, 1717
BstUI	CGCG	2 cut(s)	72, 233
BstV1I	GCAGC	8 cut(s)	909, 1806, 1909, 2506, 2626, 2758, 2903, 3082
BstV2I	GAAGAC	3 cut(s)	912, 1396, 2858
BstX2I	RGATCY	2 cut(s)	2047, 2327
BstXI	CCANNNNNNTGG	1 cut(s)	1001
BstYI	RGATCY	2 cut(s)	2047, 2327
Bsu36I	CCTNAGG	1 cut(s)	119
BsuI	GTATCC	1 cut(s)	2677
BsuRI	GGCC	8 cut(s)	117, 153, 740, 888, 2241, 2379, 2414, 2880
BtgI	CCRYGG	1 cut(s)	486
BtsCI	GGATG	5 cut(s)	89, 769, 1075, 2230, 2492
BtsI	GCAGTG	1 cut(s)	667
BveI	ACCTGC	1 cut(s)	1175
CfoI	GCGC	5 cut(s)	89, 1564, 1691, 2000, 3075
Cfr10I	RCCGGY	2 cut(s)	110, 149
Cfr13I	GGNCC	2 cut(s)	152, 1858
CseI	GACGC	4 cut(s)	186, 1028, 2162, 2830
Csp6I	GTAC	5 cut(s)	265, 843, 1236, 1744, 2343
CviQI	GTAC	5 cut(s)	265, 843, 1236, 1744, 2343
DpnI	GATC	9 cut(s)	191, 333, 1191, 1545, 1656, 2049, 2121, 2229, 2329
DpnII	GATC	9 cut(s)	189, 331, 1189, 1543, 1654, 2047, 2119, 2227, 2327
DraIII	CACNNNGTG	1 cut(s)	2351
EaeI	YGGCCR	1 cut(s)	2878
Eam1104I	CTCTTC	5 cut(s)	287, 384, 711, 2153, 2516
EarI	CTCTTC	5 cut(s)	287, 384, 711, 2153, 2516
EciI	GGCGGA	4 cut(s)	56, 89, 162, 174
Eco130I	CCWWGG	3 cut(s)	2838, 2923, 3099
Eco24I	GRGCYC	1 cut(s)	2040
Eco31I	GGTCTC	2 cut(s)	129, 2759
Eco32I	GATATC	1 cut(s)	1359
Eco47I	GGWCC	1 cut(s)	1858
Eco57I	CTGAAG	2 cut(s)	482, 1085
Eco81I	CCTNAGG	1 cut(s)	119
EcoRII	CCWGG	1 cut(s)	1844
EcoRV	GATATC	1 cut(s)	1359
EcoT14I	CCWWGG	3 cut(s)	2838, 2923, 3099
EcoT22I	ATGCAT	2 cut(s)	2306, 2512
EcoT38I	GRGCYC	1 cut(s)	2040
ErhI	CCWWGG	3 cut(s)	2838, 2923, 3099
FalI	AAGNNNNNCTT	2 cut(s)	186, 218
FaqI	GGGAC	5 cut(s)	1317, 1826, 1844, 2818, 3019
FauI	CCCGC	1 cut(s)	65
FauNDI	CATATG	1 cut(s)	1450
FbaI	TGATCA	3 cut(s)	189, 1189, 1654
FblI	GTMKAC	2 cut(s)	1386, 2730
FokI	GGATG	5 cut(s)	96, 776, 1082, 2237, 2499
FriOI	GRGCYC	1 cut(s)	2040
FspBI	CTAG	3 cut(s)	1916, 2231, 2915
FspI	TGCGCA	1 cut(s)	1690
GlaI	GCGC	5 cut(s)	88, 1563, 1690, 1999, 3074
GsaI	CCCAGC	4 cut(s)	1323, 1485, 1902, 3050
GsuI	CTGGAG	1 cut(s)	1446
HaeIII	GGCC	8 cut(s)	117, 153, 740, 888, 2241, 2379, 2414, 2880
HapII	CCGG	9 cut(s)	111, 139, 150, 156, 323, 410, 1312, 3017, 3087
HgaI	GACGC	4 cut(s)	186, 1028, 2162, 2830
HhaI	GCGC	5 cut(s)	89, 1564, 1691, 2000, 3075
Hin1I	GRCGYC	1 cut(s)	163
Hin6I	GCGC	5 cut(s)	87, 1562, 1689, 1998, 3073
HinP1I	GCGC	5 cut(s)	87, 1562, 1689, 1998, 3073
HincII	GTYRAC	2 cut(s)	1597, 2964
HindII	GTYRAC	2 cut(s)	1597, 2964
HindIII	AAGCTT	2 cut(s)	1194, 2990
HpaI	GTTAAC	1 cut(s)	2964
HpaII	CCGG	9 cut(s)	111, 139, 150, 156, 323, 410, 1312, 3017, 3087
HphI	GGTGA	8 cut(s)	250, 292, 320, 621, 1817, 2363, 2612, 2991
Hpy188III	TCNNGA	8 cut(s)	449, 642, 1274, 1587, 2231, 2562, 2737, 2868
Hpy99I	CGWCG	4 cut(s)	202, 233, 520, 1022
HpyCH4IV	ACGT	2 cut(s)	163, 242
HpySE526I	ACGT	2 cut(s)	163, 242
Hsp92I	GRCGYC	1 cut(s)	163
HspAI	GCGC	5 cut(s)	87, 1562, 1689, 1998, 3073
KroI	GCCGGC	2 cut(s)	110, 149
KroNI	GCCGGC	2 cut(s)	112, 151
Ksp22I	TGATCA	3 cut(s)	189, 1189, 1654
KspAI	GTTAAC	1 cut(s)	2964
Kzo9I	GATC	9 cut(s)	189, 331, 1189, 1543, 1654, 2047, 2119, 2227, 2327
LmnI	GCTCC	5 cut(s)	65, 141, 1589, 2253, 2502
Lsp1109I	GCAGC	8 cut(s)	909, 1806, 1909, 2506, 2626, 2758, 2903, 3082
MaeI	CTAG	3 cut(s)	1916, 2231, 2915
MaeII	ACGT	2 cut(s)	163, 242
MaeIII	GTNAC	8 cut(s)	238, 523, 595, 619, 627, 647, 2545, 3039
MalI	GATC	9 cut(s)	191, 333, 1191, 1545, 1656, 2049, 2121, 2229, 2329
MbiI	CCGCTC	1 cut(s)	60
MboI	GATC	9 cut(s)	189, 331, 1189, 1543, 1654, 2047, 2119, 2227, 2327
MflI	RGATCY	2 cut(s)	2047, 2327
MhlI	GDGCHC	6 cut(s)	993, 1273, 1459, 1675, 1717, 2040
MlsI	TGGCCA	1 cut(s)	2880
MluCI	AATT	9 cut(s)	427, 1040, 1786, 2017, 2064, 2466, 2652, 2826, 3091
MluNI	TGGCCA	1 cut(s)	2880
MlyI	GAGTC	5 cut(s)	461, 572, 635, 1079, 2366
MmeI	TCCRAC	7 cut(s)	539, 741, 1107, 1107, 1250, 1948, 2130
Mox20I	TGGCCA	1 cut(s)	2880
Mph1103I	ATGCAT	2 cut(s)	2306, 2512
MreI	CGCCGGCG	1 cut(s)	110
MroNI	GCCGGC	2 cut(s)	110, 149
MroXI	GAANNNNTTC	2 cut(s)	720, 3094
MscI	TGGCCA	1 cut(s)	2880
MslI	CAYNNNNRTG	2 cut(s)	491, 1449
Msp20I	TGGCCA	1 cut(s)	2880
MspCI	CTTAAG	1 cut(s)	2051
MspI	CCGG	9 cut(s)	111, 139, 150, 156, 323, 410, 1312, 3017, 3087
MspR9I	CCNGG	3 cut(s)	156, 1313, 1846
Mva1269I	GAATGC	1 cut(s)	859
MvaI	CCWGG	1 cut(s)	1846
MvnI	CGCG	2 cut(s)	72, 233
NaeI	GCCGGC	2 cut(s)	112, 151
NciI	CCSGG	2 cut(s)	156, 1313
NdeI	CATATG	1 cut(s)	1450
NdeII	GATC	9 cut(s)	189, 331, 1189, 1543, 1654, 2047, 2119, 2227, 2327
NgoMIV	GCCGGC	2 cut(s)	110, 149
NlaIV	GGNNCC	5 cut(s)	143, 154, 799, 1860, 2779
NmeAIII	GCCGAG	1 cut(s)	227
NmuCI	GTSAC	6 cut(s)	238, 523, 595, 619, 627, 647
NsbI	TGCGCA	1 cut(s)	1690
NsiI	ATGCAT	2 cut(s)	2306, 2512
NspI	RCATGY	8 cut(s)	730, 941, 1368, 1689, 1907, 2004, 2317, 2343
NspV	TTCGAA	1 cut(s)	3082
OliI	CACNNNNGTG	1 cut(s)	491
PaeI	GCATGC	2 cut(s)	1689, 1907
PciI	ACATGT	3 cut(s)	726, 937, 1364
PctI	GAATGC	1 cut(s)	859
PdiI	GCCGGC	2 cut(s)	112, 151
PdmI	GAANNNNTTC	2 cut(s)	720, 3094
PflFI	GACNNNGTC	1 cut(s)	2172
PflMI	CCANNNNNTGG	3 cut(s)	1414, 2019, 3008
PfoI	TCCNGGA	1 cut(s)	1311
PleI	GAGTC	5 cut(s)	460, 572, 634, 1079, 2366
PpsI	GAGTC	5 cut(s)	460, 572, 634, 1079, 2366
PscI	ACATGT	3 cut(s)	726, 937, 1364
PshAI	GACNNNNGTC	1 cut(s)	521
PshBI	ATTAAT	1 cut(s)	1886
Psp6I	CCWGG	1 cut(s)	1844
PspFI	CCCAGC	4 cut(s)	1319, 1481, 1898, 3046
PspGI	CCWGG	1 cut(s)	1844
PspN4I	GGNNCC	5 cut(s)	143, 154, 799, 1860, 2779
PspPI	GGNCC	2 cut(s)	152, 1858
PstI	CTGCAG	2 cut(s)	420, 2815
PsuI	RGATCY	2 cut(s)	2047, 2327
PsyI	GACNNNGTC	1 cut(s)	2172
RsaI	GTAC	5 cut(s)	266, 844, 1237, 1745, 2344
RsaNI	GTAC	5 cut(s)	265, 843, 1236, 1744, 2343
RseI	CAYNNNNRTG	2 cut(s)	491, 1449
Sau3AI	GATC	9 cut(s)	189, 331, 1189, 1543, 1654, 2047, 2119, 2227, 2327
Sau96I	GGNCC	2 cut(s)	152, 1858
ScaI	AGTACT	1 cut(s)	844
SchI	GAGTC	5 cut(s)	461, 572, 635, 1079, 2366
ScrFI	CCNGG	3 cut(s)	156, 1313, 1846
SduI	GDGCHC	6 cut(s)	993, 1273, 1459, 1675, 1717, 2040
SfcI	CTRYAG	5 cut(s)	416, 846, 1644, 2126, 2811
SfuI	TTCGAA	1 cut(s)	3082
SgrAI	CRCCGGYG	1 cut(s)	110
SinI	GGWCC	1 cut(s)	1858
SmiMI	CAYNNNNRTG	2 cut(s)	491, 1449
SmlI	CTYRAG	5 cut(s)	542, 1272, 2051, 2256, 2630
SmoI	CTYRAG	5 cut(s)	542, 1272, 2051, 2256, 2630
SphI	GCATGC	2 cut(s)	1689, 1907
Sse9I	AATT	9 cut(s)	427, 1040, 1786, 2017, 2064, 2466, 2652, 2826, 3091
SspMI	CTAG	3 cut(s)	1916, 2231, 2915
StyD4I	CCNGG	3 cut(s)	154, 1311, 1844
StyI	CCWWGG	3 cut(s)	2838, 2923, 3099
TaiI	ACGT	2 cut(s)	166, 245
TaqI	TCGA	7 cut(s)	215, 555, 688, 823, 1546, 1954, 3082
TasI	AATT	9 cut(s)	427, 1040, 1786, 2017, 2064, 2466, 2652, 2826, 3091
TatI	WGTACW	5 cut(s)	264, 842, 1235, 1743, 2342
TauI	GCSGC	5 cut(s)	72, 117, 178, 505, 2368
TseFI	GTSAC	6 cut(s)	238, 523, 595, 619, 627, 647
TseI	GCWGC	8 cut(s)	897, 1794, 1922, 2494, 2639, 2771, 2891, 3070
Tsp45I	GTSAC	6 cut(s)	238, 523, 595, 619, 627, 647
TspGWI	ACGGA	2 cut(s)	533, 2338
Tth111I	GACNNNGTC	1 cut(s)	2172
Van91I	CCANNNNNTGG	3 cut(s)	1414, 2019, 3008
Vha464I	CTTAAG	1 cut(s)	2051
VneI	GTGCAC	1 cut(s)	1671
VpaK11BI	GGWCC	1 cut(s)	1858
VspI	ATTAAT	1 cut(s)	1886
XapI	RAATTY	2 cut(s)	2826, 3091
XbaI	TCTAGA	1 cut(s)	2230
XceI	RCATGY	8 cut(s)	730, 941, 1368, 1689, 1907, 2004, 2317, 2343
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1852, 2203
XmiI	GTMKAC	2 cut(s)	1386, 2730
XmnI	GAANNNNTTC	2 cut(s)	720, 3094
XspI	CTAG	3 cut(s)	1916, 2231, 2915
ZraI	GACGTC	1 cut(s)	164
ZrmI	AGTACT	1 cut(s)	844
Zsp2I	ATGCAT	2 cut(s)	2306, 2512

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.