FvH4_2g04080

QWRF motif-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0005693
Physical Location & Seq
Forward (+)
3211902 .. 3219479
7578 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g04080.t1

Sequence Viewer

Length: 1962 bp
ATGGTGGCTGCCATTTCCGACCCGAAACCCACGACCCGGCCGCCGCTTCTACCCGAGAAAGACAATGCCTCCTCCGCTGCCGCCGCTGCGGCTCAACGACGCGTCGTTTCTCGCGGCAGACAGGTCTCCTCCAAGTACATGTCCACTCCTTCTCCTTCTTCCTCAACCTCAACCTCTACTTCCACCACAGTCTCTTCTTCTTCGGTTTCTTCTAGAAGATGCCCTTCGCCGCTGCTCTCCAGGTCCATGAATTCCACTCCGGCGTCGAATTCGGGCCCGAAACGGGCCCAATCCGTGGACCGGAGGCGACCCGGTACGCCGCAGACCCGACCCGAGCAGCGCCTGAGCAATGCGGGTTCCGAAGTCTCCGCCGCGACCCGGCTTTTGGTCACTTCCACGCGGAGCTTGTCGGTTTCGTTTCAGGGCGAGGCGTTTTCGCTTCCGATTAGTAAGACCAAGGCGGCGGTGGGGACTCCGGCGAGGAAGCCCACGCCGGAACGTCGGAGGTCGACGCCGGTGAGGAGGGAGGGAGGAGATCAGGTGGAGAATTCAAGACCCGGTGAGCAGCACCGGTGGCCGGGACGGAGCCGGCAGCCGGCGGTGAGTTTGAGTAGGAGTATGGAGTGTGGTGGTAGTGTGAATAATGGGGTTGGATCTGGTGTGGTAGCTAGGGCTTTGTTGTTGCAGCAGTCTATGGTTGATGAGAGTAGCAGAGGAGGATCAAGGTCAAGATCGTCTTTTGATGGTAGGTTGAGCTTGGATTTGGGGCATTTGGGTGGCAATGCAGATGCTTTGAGAGCTTCTCACCACAACCCAGATGCTAGTTTTTCGGCTAATGAGTCCTCCGTGCCGTCTGATCTCACCGCTTCCGATACAGACAGTGTCTCCTCCGGTAGCACTTCAGGTGTTCAGGACTCTAATGGGGCTGCCAAGAGCCGAGGAGGAACTGCCGTCCCTCGCGGCATTGCTGTGTCTGCAAGGTTCTGGCAGGAGACTAATAGCAGGTTGCGGCGCTTGCAGGACCCCGGGTCACCTCTGGCTACTAGTCCTGTGTCGAGAGCAGGGGCCGCGGGGAAGTACATTCAGTCGAAGAAGTTTAATGGTGGTGATAGCAACGCTGTGTCGTCTCCCAGGACAATGTCTTCTCCCATTAGAGGAGCTACTAGGCCTGCTTCGCCCGGGAAGCTGTGGACGTCTTCGTCTTTGTCACCGTCCAGGGGGAGTGCTAGTCCTTCTCGGGCGAGGAATAGTTTTAGTGGTCCGGGTCAGTTGAGTAGTGGTTATGCTGCTAGTACGCCTTCGTTTCTTAGTTTCTCTATTGATACTCGGAGAGGGAAGAAGGGGGAAGACCGCATTGTTGACGCACACATGCTCCGGCTTCTGTATAACCGTTATGTGCAGTGGCGGTTTGTAAATGCAAGGGCAGATGCTACATACATGGTGCAGAGAGTGAATGCAGAGGAAAATCTTTGGAATGCATGGGTAACAATCTCAGAGCTACGGCATAATGTCACACTTAAAAGGGTCAAGTTACTCTTATTGAGGCATAAGTTGAAGCTAACTTCTATCCTCAAAGGACAAATCACGTATTTGGAAGAATGGGCTCTTCTGGATAGAGATCACTCTAGTTCTTTGCTAGGAGCGAATGAAGCTTTGCAAGCCAGTACCCTCCGTCTTCCGGTTGTTGGAAAAGCAATAGTTGACTTTCAAAAGCTGAAAGATTCTGTGGTCTCAGCAGCTGATGTGATGCAGGCAATGACATCCTCGATATGCTCTCTTTCATCAAAGGTGGAAGAAATGAATTCCTTGGTATCTGAACTTGTGAAGGTGACTACAACAGAACGTCTTGTGCTTGAACAATGCAAAGATTTTTTGTCGACGTTATCGGCCATGCAAGTCAAGGACTGTAGCTTGAGAACACATATAATTCAACTAAAACGTCTACCATTACCCGCAGCCTGA

Protein Analysis

654

Amino Acids

69.84

Weight (kDa)

10.8

Isoelectric Point (pI)

61.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
QWRF PF04484 294 - 619 3.9e-113 QWRF family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1198
AatII GACGTC 1 cut(s) 1196
Acc36I ACCTGC 1 cut(s) 993
AccB7I CCANNNNNTGG 1 cut(s) 295
AccI GTMKAC 3 cut(s) 509, 1877, 1942
AccII CGCG 6 cut(s) 102, 114, 374, 400, 960, 1070
AclWI GGATC 2 cut(s) 661, 727
AcoI YGGCCR 3 cut(s) 38, 575, 1887
AcsI RAATTY 4 cut(s) 250, 268, 547, 1801
AcuI CTGAAG 1 cut(s) 885
AcyI GRCGYC 3 cut(s) 263, 512, 1193
AfaI GTAC 5 cut(s) 137, 316, 1079, 1294, 1666
AflIII ACRYGT 2 cut(s) 100, 138
AgeI ACCGGT 1 cut(s) 570
AgsI TTSAA 5 cut(s) 552, 1555, 1709, 1856, 1931
AhdI GACNNNNNGTC 1 cut(s) 1027
AhlI ACTAGT 1 cut(s) 1043
AjnI CCWGG 3 cut(s) 239, 1130, 1214
Alw26I GTCTC 7 cut(s) 130, 196, 370, 889, 986, 1131, 1735
AlwI GGATC 2 cut(s) 661, 727
AlwNI CAGNNNCTG 2 cut(s) 343, 1739
Ama87I CYCGRG 5 cut(s) 53, 332, 1025, 1178, 1236
AoxI GGCC 7 cut(s) 38, 274, 285, 575, 1065, 1166, 1887
ApaI GGGCCC 2 cut(s) 278, 289
ApoI RAATTY 4 cut(s) 250, 268, 547, 1801
ArsI GACNNNNNNTTYG 2 cut(s) 367, 399
AsiGI ACCGGT 1 cut(s) 570
AspLEI GCGC 2 cut(s) 342, 1014
AspS9I GGNCC 9 cut(s) 243, 274, 275, 285, 286, 298, 1021, 1065, 1259
AsuHPI GGTGA 9 cut(s) 529, 572, 613, 797, 853, 1023, 1118, 1200, 1840
AvaI CYCGRG 5 cut(s) 53, 332, 1025, 1178, 1236
AvaII GGWCC 4 cut(s) 243, 298, 1021, 1259
BaeGI GKGCMC 2 cut(s) 278, 289
BanII GRGCYC 3 cut(s) 278, 289, 1606
BbsI GAAGAC 4 cut(s) 1134, 1188, 1353, 1667
BccI CCATC 1 cut(s) 737
BceAI ACGGC 3 cut(s) 835, 935, 1517
BciT130I CCWGG 3 cut(s) 241, 1132, 1216
BcoDI GTCTC 7 cut(s) 130, 196, 370, 889, 986, 1131, 1735
BcuI ACTAGT 1 cut(s) 1043
BfaI CTAG 9 cut(s) 213, 669, 822, 1044, 1164, 1227, 1290, 1626, 1637
BfmI CTRYAG 1 cut(s) 1906
BfoI RGCGCY 2 cut(s) 343, 1015
BfuAI ACCTGC 1 cut(s) 993
BglI GCCNNNNNGGC 1 cut(s) 89
Bme18I GGWCC 4 cut(s) 243, 298, 1021, 1259
BmeRI GACNNNNNGTC 1 cut(s) 1027
BmeT110I CYCGRG 5 cut(s) 53, 332, 1025, 1178, 1236
BmgT120I GGNCC 9 cut(s) 243, 274, 275, 285, 286, 298, 1021, 1065, 1259
BmiI GGNNCC 6 cut(s) 276, 287, 358, 587, 1023, 1066
BmsI GCATC 5 cut(s) 209, 778, 808, 1417, 1737
BpiI GAAGAC 4 cut(s) 1134, 1188, 1353, 1667
BplI GAGNNNNNCTC 2 cut(s) 787, 819
BpmI CTGGAG 1 cut(s) 223
Bpu10I CCTNAGC 1 cut(s) 344
BpuEI CTTGAG 1 cut(s) 1933
BsaAI YACGTR 1 cut(s) 1587
BsaBI GATNNNNATC 1 cut(s) 1617
BsaHI GRCGYC 3 cut(s) 263, 512, 1193
BsaI GGTCTC 2 cut(s) 130, 1735
BsaWI WCCGGW 4 cut(s) 300, 570, 890, 1678
Bse118I RCCGGY 4 cut(s) 514, 570, 588, 595
Bse1I ACTGG 1 cut(s) 1662
Bse3DI GCAATG 4 cut(s) 355, 787, 963, 1761
Bse8I GATNNNNATC 1 cut(s) 1617
BseBI CCWGG 3 cut(s) 241, 1132, 1216
BseGI GGATG 1 cut(s) 1760
BseJI GATNNNNATC 1 cut(s) 1617
BseMI GCAATG 4 cut(s) 355, 787, 963, 1761
BseMII CTCAG 3 cut(s) 335, 1506, 1746
BseNI ACTGG 1 cut(s) 1662
BseRI GAGGAG 8 cut(s) 61, 118, 535, 546, 729, 877, 954, 1170
BseSI GKGCMC 2 cut(s) 278, 289
BseX3I CGGCCG 1 cut(s) 38
BsgI GTGCAG 2 cut(s) 1418, 1463
Bsh1236I CGCG 6 cut(s) 102, 114, 374, 400, 960, 1070
Bsh1285I CGRYCG 1 cut(s) 41
BshFI GGCC 7 cut(s) 40, 276, 287, 577, 1067, 1168, 1889
BshTI ACCGGT 1 cut(s) 570
BsiEI CGRYCG 1 cut(s) 41
BsiHKCI CYCGRG 5 cut(s) 53, 332, 1025, 1178, 1236
BslFI GGGAC 3 cut(s) 484, 594, 938
BsmAI GTCTC 7 cut(s) 130, 196, 370, 889, 986, 1131, 1735
BsmBI CGTCTC 1 cut(s) 1131
BsmFI GGGAC 3 cut(s) 484, 594, 938
BsmI GAATGC 2 cut(s) 1459, 1480
BsnI GGCC 7 cut(s) 40, 276, 287, 577, 1067, 1168, 1889
Bso31I GGTCTC 2 cut(s) 130, 1735
BsoBI CYCGRG 5 cut(s) 53, 332, 1025, 1178, 1236
Bsp120I GGGCCC 2 cut(s) 274, 285
Bsp1286I GDGCHC 3 cut(s) 278, 289, 1606
Bsp143I GATC 6 cut(s) 535, 653, 719, 731, 856, 1618
BspANI GGCC 7 cut(s) 40, 276, 287, 577, 1067, 1168, 1889
BspCNI CTCAG 3 cut(s) 336, 1505, 1745
BspFNI CGCG 6 cut(s) 102, 114, 374, 400, 960, 1070
BspLI GGNNCC 6 cut(s) 276, 287, 358, 587, 1023, 1066
BspMI ACCTGC 1 cut(s) 993
BspPI GGATC 2 cut(s) 661, 727
BspQI GCTCTTC 1 cut(s) 1611
BspTNI GGTCTC 2 cut(s) 130, 1735
BsrDI GCAATG 4 cut(s) 355, 787, 963, 1761
BsrFI RCCGGY 4 cut(s) 514, 570, 588, 595
BsrI ACTGG 1 cut(s) 1662
BssAI RCCGGY 4 cut(s) 514, 570, 588, 595
BssMI GATC 6 cut(s) 535, 653, 719, 731, 856, 1618
BssNI GRCGYC 3 cut(s) 263, 512, 1193
BssT1I CCWWGG 2 cut(s) 456, 1806
Bst2UI CCWGG 3 cut(s) 241, 1132, 1216
Bst4CI ACNGT 5 cut(s) 190, 881, 1212, 1391, 1907
Bst6I CTCTTC 2 cut(s) 199, 1611
BstACI GRCGYC 3 cut(s) 263, 512, 1193
BstBAI YACGTR 1 cut(s) 1587
BstC8I GCNNGC 6 cut(s) 590, 597, 1016, 1170, 1659, 1752
BstDEI CTNAG 4 cut(s) 344, 1307, 1492, 1732
BstDSI CCRYGG 2 cut(s) 294, 1068
BstEII GGTNACC 1 cut(s) 1029
BstF5I GGATG 1 cut(s) 1760
BstFNI CGCG 6 cut(s) 102, 114, 374, 400, 960, 1070
BstH2I RGCGCY 2 cut(s) 343, 1015
BstHHI GCGC 2 cut(s) 342, 1014
BstKTI GATC 6 cut(s) 538, 656, 722, 734, 859, 1621
BstMAI GTCTC 7 cut(s) 130, 196, 370, 889, 986, 1131, 1735
BstMBI GATC 6 cut(s) 535, 653, 719, 731, 856, 1618
BstMCI CGRYCG 1 cut(s) 41
BstNI CCWGG 3 cut(s) 241, 1132, 1216
BstNSI RCATGY 2 cut(s) 142, 1372
BstPI GGTNACC 1 cut(s) 1029
BstSFI CTRYAG 1 cut(s) 1906
BstSLI GKGCMC 2 cut(s) 278, 289
BstUI CGCG 6 cut(s) 102, 114, 374, 400, 960, 1070
BstV2I GAAGAC 4 cut(s) 1134, 1188, 1353, 1667
BstX2I RGATCY 1 cut(s) 653
BstYI RGATCY 1 cut(s) 653
BstZI CGGCCG 1 cut(s) 38
BsuRI GGCC 7 cut(s) 40, 276, 287, 577, 1067, 1168, 1889
BtgI CCRYGG 2 cut(s) 294, 1068
BtsCI GGATG 1 cut(s) 1760
BtsI GCAGTG 1 cut(s) 1406
BtsIMutI CAGTG 2 cut(s) 886, 1406
BveI ACCTGC 1 cut(s) 993
Cac8I GCNNGC 6 cut(s) 590, 597, 1016, 1170, 1659, 1752
CaiI CAGNNNCTG 2 cut(s) 343, 1739
CfoI GCGC 2 cut(s) 342, 1014
Cfr10I RCCGGY 4 cut(s) 514, 570, 588, 595
Cfr13I GGNCC 9 cut(s) 243, 274, 275, 285, 286, 298, 1021, 1065, 1259
Cfr42I CCGCGG 1 cut(s) 1071
Cfr9I CCCGGG 2 cut(s) 1025, 1178
CseI GACGC 5 cut(s) 91, 108, 252, 520, 1370
Csp6I GTAC 5 cut(s) 136, 315, 1078, 1293, 1665
CspAI ACCGGT 1 cut(s) 570
CviAII CATG 6 cut(s) 139, 247, 1369, 1438, 1479, 1891
CviQI GTAC 5 cut(s) 136, 315, 1078, 1293, 1665
DdeI CTNAG 4 cut(s) 344, 1307, 1492, 1732
DpnI GATC 6 cut(s) 537, 655, 721, 733, 858, 1620
DpnII GATC 6 cut(s) 535, 653, 719, 731, 856, 1618
DrdI GACNNNNNNGTC 1 cut(s) 1198
DriI GACNNNNNGTC 1 cut(s) 1027
DseDI GACNNNNNNGTC 1 cut(s) 1198
EaeI YGGCCR 3 cut(s) 38, 575, 1887
EagI CGGCCG 1 cut(s) 38
Eam1104I CTCTTC 2 cut(s) 199, 1611
Eam1105I GACNNNNNGTC 1 cut(s) 1027
EarI CTCTTC 2 cut(s) 199, 1611
EciI GGCGGA 1 cut(s) 358
EclXI CGGCCG 1 cut(s) 38
Eco130I CCWWGG 2 cut(s) 456, 1806
Eco147I AGGCCT 1 cut(s) 1168
Eco24I GRGCYC 3 cut(s) 278, 289, 1606
Eco31I GGTCTC 2 cut(s) 130, 1735
Eco47I GGWCC 4 cut(s) 243, 298, 1021, 1259
Eco52I CGGCCG 1 cut(s) 38
Eco57I CTGAAG 1 cut(s) 885
Eco88I CYCGRG 5 cut(s) 53, 332, 1025, 1178, 1236
Eco91I GGTNACC 1 cut(s) 1029
EcoO109I RGGNCCY 1 cut(s) 1021
EcoO65I GGTNACC 1 cut(s) 1029
EcoRI GAATTC 4 cut(s) 250, 268, 547, 1801
EcoRII CCWGG 3 cut(s) 239, 1130, 1214
EcoT14I CCWWGG 2 cut(s) 456, 1806
EcoT22I ATGCAT 1 cut(s) 1480
EcoT38I GRGCYC 3 cut(s) 278, 289, 1606
ErhI CCWWGG 2 cut(s) 456, 1806
Esp3I CGTCTC 1 cut(s) 1131
FaeI CATG 6 cut(s) 142, 250, 1372, 1441, 1482, 1894
FalI AAGNNNNNCTT 6 cut(s) 208, 240, 721, 753, 1520, 1552
FaqI GGGAC 3 cut(s) 484, 594, 938
FatI CATG 6 cut(s) 138, 246, 1368, 1437, 1478, 1890
FauI CCCGC 3 cut(s) 346, 1063, 1960
FblI GTMKAC 3 cut(s) 509, 1877, 1942
FokI GGATG 1 cut(s) 1747
FriOI GRGCYC 3 cut(s) 278, 289, 1606
FspBI CTAG 9 cut(s) 213, 669, 822, 1044, 1164, 1227, 1290, 1626, 1637
GlaI GCGC 2 cut(s) 341, 1013
GsuI CTGGAG 1 cut(s) 223
HaeII RGCGCY 2 cut(s) 343, 1015
HaeIII GGCC 7 cut(s) 40, 276, 287, 577, 1067, 1168, 1889
HgaI GACGC 5 cut(s) 91, 108, 252, 520, 1370
HhaI GCGC 2 cut(s) 342, 1014
Hin1I GRCGYC 3 cut(s) 263, 512, 1193
Hin1II CATG 6 cut(s) 142, 250, 1372, 1441, 1482, 1894
Hin6I GCGC 2 cut(s) 340, 1012
HinP1I GCGC 2 cut(s) 340, 1012
HincII GTYRAC 4 cut(s) 510, 1360, 1702, 1878
HindII GTYRAC 4 cut(s) 510, 1360, 1702, 1878
HindIII AAGCTT 1 cut(s) 1650
HinfI GANTC 4 cut(s) 472, 839, 914, 1721
HphI GGTGA 9 cut(s) 529, 572, 613, 797, 853, 1023, 1118, 1200, 1840
Hpy166II GTNNAC 8 cut(s) 144, 298, 510, 1191, 1360, 1702, 1878, 1943
Hpy188I TCNGA 9 cut(s) 19, 361, 444, 504, 856, 871, 1329, 1495, 1816
Hpy188III TCNNGA 6 cut(s) 213, 552, 729, 911, 1056, 1610
Hpy8I GTNNAC 8 cut(s) 144, 298, 510, 1191, 1360, 1702, 1878, 1943
Hpy99I CGWCG 6 cut(s) 102, 107, 268, 504, 514, 1882
HpyAV CCTTC 7 cut(s) 159, 165, 234, 1242, 1308, 1333, 1819
HpyCH4III ACNGT 5 cut(s) 190, 881, 1212, 1391, 1907
HpyCH4IV ACGT 6 cut(s) 499, 1193, 1586, 1843, 1880, 1939
HpyF3I CTNAG 4 cut(s) 344, 1307, 1492, 1732
HpySE526I ACGT 6 cut(s) 499, 1193, 1586, 1843, 1880, 1939
Hsp92I GRCGYC 3 cut(s) 263, 512, 1193
Hsp92II CATG 6 cut(s) 142, 250, 1372, 1441, 1482, 1894
HspAI GCGC 2 cut(s) 340, 1012
KroI GCCGGC 2 cut(s) 588, 595
KroNI GCCGGC 2 cut(s) 590, 597
KspI CCGCGG 1 cut(s) 1071
Kzo9I GATC 6 cut(s) 535, 653, 719, 731, 856, 1618
LguI GCTCTTC 1 cut(s) 1611
LmnI GCTCC 5 cut(s) 402, 585, 1157, 1377, 1640
LweI GCATC 5 cut(s) 209, 778, 808, 1417, 1737
MaeI CTAG 9 cut(s) 213, 669, 822, 1044, 1164, 1227, 1290, 1626, 1637
MaeII ACGT 6 cut(s) 499, 1193, 1586, 1843, 1880, 1939
MaeIII GTNAC 7 cut(s) 388, 1029, 1206, 1483, 1510, 1530, 1828
MalI GATC 6 cut(s) 537, 655, 721, 733, 858, 1620
MboI GATC 6 cut(s) 535, 653, 719, 731, 856, 1618
MflI RGATCY 1 cut(s) 653
MhlI GDGCHC 3 cut(s) 278, 289, 1606
MluCI AATT 5 cut(s) 250, 268, 547, 1801, 1926
MluI ACGCGT 1 cut(s) 100
MlyI GAGTC 3 cut(s) 466, 848, 908
MmeI TCCRAC 4 cut(s) 42, 482, 631, 1666
Mph1103I ATGCAT 1 cut(s) 1480
MroNI GCCGGC 2 cut(s) 588, 595
MseI TTAA 2 cut(s) 1098, 1518
MslI CAYNNNNRTG 2 cut(s) 774, 968
MspA1I CMGCKG 5 cut(s) 77, 86, 232, 1070, 1739
Mva1269I GAATGC 2 cut(s) 1459, 1480
MvaI CCWGG 3 cut(s) 241, 1132, 1216
MvnI CGCG 6 cut(s) 102, 114, 374, 400, 960, 1070
NaeI GCCGGC 2 cut(s) 590, 597
NdeII GATC 6 cut(s) 535, 653, 719, 731, 856, 1618
NgoMIV GCCGGC 2 cut(s) 588, 595
NlaIII CATG 6 cut(s) 142, 250, 1372, 1441, 1482, 1894
NlaIV GGNNCC 6 cut(s) 276, 287, 358, 587, 1023, 1066
NmeAIII GCCGAG 1 cut(s) 962
NmuCI GTSAC 5 cut(s) 388, 1029, 1206, 1510, 1828
NsiI ATGCAT 1 cut(s) 1480
NspI RCATGY 2 cut(s) 142, 1372
PceI AGGCCT 1 cut(s) 1168
PciI ACATGT 1 cut(s) 138
PciSI GCTCTTC 1 cut(s) 1611
PctI GAATGC 2 cut(s) 1459, 1480
PdiI GCCGGC 2 cut(s) 590, 597
PfeI GAWTC 1 cut(s) 1721
PflFI GACNNNGTC 2 cut(s) 881, 1138
PflMI CCANNNNNTGG 1 cut(s) 295
PinAI ACCGGT 1 cut(s) 570
PleI GAGTC 3 cut(s) 466, 847, 908
PpsI GAGTC 3 cut(s) 466, 847, 908
Ppu21I YACGTR 1 cut(s) 1587
PpuMI RGGWCCY 1 cut(s) 1021
PscI ACATGT 1 cut(s) 138
Psp5II RGGWCCY 1 cut(s) 1021
Psp6I CCWGG 3 cut(s) 239, 1130, 1214
PspEI GGTNACC 1 cut(s) 1029
PspGI CCWGG 3 cut(s) 239, 1130, 1214
PspN4I GGNNCC 6 cut(s) 276, 287, 358, 587, 1023, 1066
PspOMI GGGCCC 2 cut(s) 274, 285
PspPI GGNCC 9 cut(s) 243, 274, 275, 285, 286, 298, 1021, 1065, 1259
PspPPI RGGWCCY 1 cut(s) 1021
PstNI CAGNNNCTG 2 cut(s) 343, 1739
PsuI RGATCY 1 cut(s) 653
PsyI GACNNNGTC 2 cut(s) 881, 1138
PvuII CAGCTG 1 cut(s) 1739
RsaI GTAC 5 cut(s) 137, 316, 1079, 1294, 1666
RsaNI GTAC 5 cut(s) 136, 315, 1078, 1293, 1665
RseI CAYNNNNRTG 2 cut(s) 774, 968
SacII CCGCGG 1 cut(s) 1071
SalI GTCGAC 2 cut(s) 508, 1876
SapI GCTCTTC 1 cut(s) 1611
SaqAI TTAA 2 cut(s) 1098, 1518
Sau3AI GATC 6 cut(s) 535, 653, 719, 731, 856, 1618
Sau96I GGNCC 9 cut(s) 243, 274, 275, 285, 286, 298, 1021, 1065, 1259
SchI GAGTC 3 cut(s) 466, 848, 908
SduI GDGCHC 3 cut(s) 278, 289, 1606
SfaNI GCATC 5 cut(s) 209, 778, 808, 1417, 1737
SfcI CTRYAG 1 cut(s) 1906
Sfr303I CCGCGG 1 cut(s) 1071
SgrAI CRCCGGYG 2 cut(s) 514, 570
SgrBI CCGCGG 1 cut(s) 1071
SinI GGWCC 4 cut(s) 243, 298, 1021, 1259
SmaI CCCGGG 2 cut(s) 1027, 1180
SmiMI CAYNNNNRTG 2 cut(s) 774, 968
SmlI CTYRAG 1 cut(s) 1912
SmoI CTYRAG 1 cut(s) 1912
SpeI ACTAGT 1 cut(s) 1043
Sse9I AATT 5 cut(s) 250, 268, 547, 1801, 1926
SseBI AGGCCT 1 cut(s) 1168
SspMI CTAG 9 cut(s) 213, 669, 822, 1044, 1164, 1227, 1290, 1626, 1637
StuI AGGCCT 1 cut(s) 1168
StyI CCWWGG 2 cut(s) 456, 1806
TaaI ACNGT 5 cut(s) 190, 881, 1212, 1391, 1907
TaiI ACGT 6 cut(s) 502, 1196, 1589, 1846, 1883, 1942
TaqI TCGA 6 cut(s) 266, 509, 1055, 1088, 1766, 1877
TasI AATT 5 cut(s) 250, 268, 547, 1801, 1926
TatI WGTACW 2 cut(s) 135, 1077
TfiI GAWTC 1 cut(s) 1721
Tru1I TTAA 2 cut(s) 1098, 1518
Tru9I TTAA 2 cut(s) 1098, 1518
TscAI CASTG 2 cut(s) 886, 1406
TseFI GTSAC 5 cut(s) 388, 1029, 1206, 1510, 1828
Tsp45I GTSAC 5 cut(s) 388, 1029, 1206, 1510, 1828
TspDTI ATGAA 4 cut(s) 263, 1662, 1770, 1814
TspGWI ACGGA 4 cut(s) 283, 598, 835, 1661
TspMI CCCGGG 2 cut(s) 1025, 1178
TspRI CASTG 2 cut(s) 886, 1406
Tth111I GACNNNGTC 2 cut(s) 881, 1138
Van91I CCANNNNNTGG 1 cut(s) 295
VpaK11BI GGWCC 4 cut(s) 243, 298, 1021, 1259
XapI RAATTY 4 cut(s) 250, 268, 547, 1801
XbaI TCTAGA 1 cut(s) 212
XceI RCATGY 2 cut(s) 142, 1372
XcmI CCANNNNNNNNNTGG 1 cut(s) 463
XmaI CCCGGG 2 cut(s) 1025, 1178
XmiI GTMKAC 3 cut(s) 509, 1877, 1942
XspI CTAG 9 cut(s) 213, 669, 822, 1044, 1164, 1227, 1290, 1626, 1637
ZraI GACGTC 1 cut(s) 1194
Zsp2I ATGCAT 1 cut(s) 1480
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.