MD05G1097600.v1.1

QWRF motif-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0005693
Physical Location & Seq
Forward (+)
20336650 .. 20337962
1313 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1097600.v1.1.491

Sequence Viewer

Length: 1293 bp
ATGTCTCCTTCGCCTTCTTCTTCTTCGACCTCGACCTCCACTTCTACGGTCTCGTCCTCTGCGTCTTCCAGAAGATGCCAATCTCCGTTGCTCTCAAGGTCCATAAACTCGGCTTCGAACTCGACCCCCGTTCCGGCTCAGAAACGGGCTCAATCGGCGGACCGGCGGCGACCCATTACTCCTCGGACCTCCGCCGGCCTCCCCGAGGCTCGGGTGAGCAATGCCGGTTCCGAAGTCTCTGCTGCAACTCGGCTTTTGGTCACTTCCACTCGGAGCTTGTCGGTTTCGTTCCAAGGTGAGGCCTTTTCGCTTCCGATTAGCAAGACTAAGACTCCAGCCTCGCCGAGTGGGACTGCCGCGAGGAAGGCCACGCCTGAGAGACGCCGGTCGACCCCTGTGAGAGGAGATCAAGCTGAGAATTCTAAGCCGTCTGATCAGCACCGGTGGCCGGCCAGGACTAGACAGTTGAGCTCGGGTGGGACTAATAACTTGTCCAGGAGCATGGACTGCAGTTCGGACAACAGGAAGCTCAGTGGGATTGGATCTGGGATTGTGGGTCGAGCACTGCAGCAATCCATGATTGATGAGAGCAGAAGAGCGTCTTTCGATGGTAGATTGAGCTTGGATTTGGGGAATGCTGAGCTTTTGAAGGATGCTAGGCAAAACCCAGATGCTAATTCTGCCCATGAATCCTCTGTGCCGTCTGATCTCACTGCTTCTGATACAGACAGTGTTTCTTCTGGTAGCACTTCAGGTGTGCAGGATGCTGGTGGTGCCGAAAAGGGTCGAACCTTACCCCGTGGCATTGCGGTGTCAGCAAGGTTTTGGCAGGAGACTAATAGCCGGTTGAGGCGGTTGCAGGGTCCTGGTTCGCCTTTATCAACAAGCCCTGTGTCTAGAGCAGGGGCAAAATTCGTTCAATCTCAATCGAAAAGGCTTAATGGTGATGTCCCATTATCATCATCTCCCCGGACAATGGCTTCCCCCACTAGGGGACCAACTAGGCCTGCTTCGCCGGGTAAGCTTTGGACTTCTTCATCAATGTCACCATCAAGGGGTTATAGTCCTTCTCGTAGAAGTAGTGTTGAAAGTACCTTGAATAGGAGTTATTCTGGTCCTGCACCTTCAATCCTTAGTTTTTCTGTTGATACTCGGAGAGGGAAGATGGGGGAAGATAGGATTGTTGATGCACACATGCTCAGGCTTCTGTATAACCGTTATCTGCAATGGCGGTTTGTAAATGCAAGATCAGATGCTACCTTCATGGTGCAGAGACGAAATGCCGAGGTATGA

Protein Analysis

431

Amino Acids

45.88

Weight (kDa)

11.48

Isoelectric Point (pI)

75.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
QWRF PF04484 244 - 429 1.4e-44 QWRF family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 773
AccI GTMKAC 1 cut(s) 389
AccII CGCG 1 cut(s) 359
AciI CCGC 7 cut(s) 158, 166, 192, 357, 809, 853, 1231
AclWI GGATC 1 cut(s) 550
AcoI YGGCCR 2 cut(s) 446, 450
AcsI RAATTY 2 cut(s) 418, 911
AcuI CTGAAG 1 cut(s) 735
AcyI GRCGYC 1 cut(s) 382
AfaI GTAC 1 cut(s) 1093
AgeI ACCGGT 1 cut(s) 441
AgsI TTSAA 5 cut(s) 649, 920, 1088, 1099, 1128
AjnI CCWGG 3 cut(s) 452, 494, 865
AluBI AGCT 7 cut(s) 276, 413, 471, 529, 621, 643, 1024
AluI AGCT 7 cut(s) 276, 413, 471, 529, 621, 643, 1024
Alw21I GWGCWC 2 cut(s) 473, 565
Alw26I GTCTC 6 cut(s) 9, 55, 241, 373, 827, 1267
AlwI GGATC 1 cut(s) 550
Ama87I CYCGRG 3 cut(s) 203, 210, 472
AoxI GGCC 6 cut(s) 196, 300, 366, 446, 450, 1004
ApeKI GCWGC 2 cut(s) 242, 568
ApoI RAATTY 2 cut(s) 418, 911
AsiGI ACCGGT 1 cut(s) 441
AspS9I GGNCC 6 cut(s) 99, 160, 186, 863, 995, 1115
AsuC2I CCSGG 2 cut(s) 970, 1017
AsuHPI GGTGA 4 cut(s) 226, 308, 956, 1038
AsuII TTCGAA 1 cut(s) 116
AvaI CYCGRG 3 cut(s) 203, 210, 472
AvaII GGWCC 6 cut(s) 99, 160, 186, 863, 995, 1115
BanI GGYRCC 1 cut(s) 773
BanII GRGCYC 2 cut(s) 151, 473
BbsI GAAGAC 1 cut(s) 57
Bbv12I GWGCWC 2 cut(s) 473, 565
BbvI GCAGC 2 cut(s) 229, 580
BccI CCATC 3 cut(s) 602, 1057, 1159
BceAI ACGGC 2 cut(s) 412, 685
BcgI CGANNNNNNTGC 2 cut(s) 221, 255
BciT130I CCWGG 3 cut(s) 454, 496, 867
BclI TGATCA 1 cut(s) 433
BcnI CCSGG 2 cut(s) 970, 1017
BcoDI GTCTC 6 cut(s) 9, 55, 241, 373, 827, 1267
BfaI CTAG 5 cut(s) 459, 657, 897, 990, 1002
BfmI CTRYAG 2 cut(s) 508, 566
BisI GCNGC 4 cut(s) 167, 243, 357, 569
BlpI GCTNAGC 1 cut(s) 639
BlsI GCNGC 4 cut(s) 168, 244, 358, 570
Bme1390I CCNGG 5 cut(s) 454, 496, 867, 970, 1017
Bme18I GGWCC 6 cut(s) 99, 160, 186, 863, 995, 1115
BmeT110I CYCGRG 3 cut(s) 203, 210, 472
BmgT120I GGNCC 6 cut(s) 99, 160, 186, 863, 995, 1115
BmiI GGNNCC 4 cut(s) 229, 775, 864, 996
BmrFI CCNGG 5 cut(s) 454, 496, 867, 970, 1017
BmsI GCATC 6 cut(s) 65, 643, 661, 754, 1177, 1243
BoxI GACNNNNGTC 1 cut(s) 385
BpiI GAAGAC 1 cut(s) 57
BpmI CTGGAG 1 cut(s) 318
Bpu10I CCTNAGC 1 cut(s) 1199
Bpu1102I GCTNAGC 1 cut(s) 639
Bpu14I TTCGAA 1 cut(s) 116
BpuEI CTTGAG 1 cut(s) 79
BpuMI CCSGG 2 cut(s) 970, 1017
BsaBI GATNNNNATC 1 cut(s) 79
BsaHI GRCGYC 1 cut(s) 382
BsaI GGTCTC 1 cut(s) 55
BsaJI CCNNGG 6 cut(s) 182, 204, 292, 799, 968, 1284
BsaWI WCCGGW 1 cut(s) 441
BsaXI ACNNNNNCTCC 2 cut(s) 316, 346
Bse118I RCCGGY 7 cut(s) 162, 194, 224, 384, 441, 448, 843
Bse3DI GCAATG 3 cut(s) 226, 804, 1232
Bse8I GATNNNNATC 1 cut(s) 79
BseBI CCWGG 3 cut(s) 454, 496, 867
BseDI CCNNGG 6 cut(s) 182, 204, 292, 799, 968, 1284
BseGI GGATG 2 cut(s) 658, 769
BseJI GATNNNNATC 1 cut(s) 79
BseMI GCAATG 3 cut(s) 226, 804, 1232
BseMII CTCAG 6 cut(s) 152, 366, 405, 544, 630, 1213
BseRI GAGGAG 2 cut(s) 171, 417
BseXI GCAGC 2 cut(s) 229, 580
BsgI GTGCAG 3 cut(s) 779, 1104, 1289
Bsh1236I CGCG 1 cut(s) 359
Bsh1285I CGRYCG 1 cut(s) 389
BshFI GGCC 6 cut(s) 198, 302, 368, 448, 452, 1006
BshNI GGYRCC 1 cut(s) 773
BshTI ACCGGT 1 cut(s) 441
BsiEI CGRYCG 1 cut(s) 389
BsiHKAI GWGCWC 2 cut(s) 473, 565
BsiHKCI CYCGRG 3 cut(s) 203, 210, 472
BslFI GGGAC 4 cut(s) 364, 493, 935, 1008
BsmAI GTCTC 6 cut(s) 9, 55, 241, 373, 827, 1267
BsmBI CGTCTC 2 cut(s) 373, 1267
BsmFI GGGAC 4 cut(s) 364, 493, 935, 1008
BsmI GAATGC 1 cut(s) 640
BsnI GGCC 6 cut(s) 198, 302, 368, 448, 452, 1006
Bso31I GGTCTC 1 cut(s) 55
BsoBI CYCGRG 3 cut(s) 203, 210, 472
Bsp119I TTCGAA 1 cut(s) 116
Bsp1286I GDGCHC 3 cut(s) 151, 473, 565
Bsp143I GATC 5 cut(s) 406, 433, 542, 706, 1247
Bsp1720I GCTNAGC 1 cut(s) 639
BspACI CCGC 7 cut(s) 158, 166, 192, 357, 809, 853, 1231
BspANI GGCC 6 cut(s) 198, 302, 368, 448, 452, 1006
BspCNI CTCAG 6 cut(s) 151, 367, 406, 543, 631, 1212
BspFNI CGCG 1 cut(s) 359
BspLI GGNNCC 4 cut(s) 229, 775, 864, 996
BspMAI CTGCAG 2 cut(s) 512, 570
BspPI GGATC 1 cut(s) 550
BspQI GCTCTTC 1 cut(s) 589
BspT104I TTCGAA 1 cut(s) 116
BspT107I GGYRCC 1 cut(s) 773
BspTNI GGTCTC 1 cut(s) 55
BsrDI GCAATG 3 cut(s) 226, 804, 1232
BsrFI RCCGGY 7 cut(s) 162, 194, 224, 384, 441, 448, 843
BssAI RCCGGY 7 cut(s) 162, 194, 224, 384, 441, 448, 843
BssECI CCNNGG 6 cut(s) 182, 204, 292, 799, 968, 1284
BssMI GATC 5 cut(s) 406, 433, 542, 706, 1247
BssNI GRCGYC 1 cut(s) 382
BssT1I CCWWGG 1 cut(s) 292
Bst2UI CCWGG 3 cut(s) 454, 496, 867
Bst4CI ACNGT 4 cut(s) 49, 465, 731, 1217
Bst6I CTCTTC 1 cut(s) 589
BstACI GRCGYC 1 cut(s) 382
BstAPI GCANNNNNTGC 1 cut(s) 507
BstBI TTCGAA 1 cut(s) 116
BstC8I GCNNGC 3 cut(s) 196, 450, 1008
BstDEI CTNAG 9 cut(s) 138, 327, 375, 414, 423, 530, 639, 1133, 1199
BstDSI CCRYGG 1 cut(s) 799
BstENI CCTNNNNNAGG 3 cut(s) 203, 399, 1099
BstF5I GGATG 2 cut(s) 658, 769
BstFNI CGCG 1 cut(s) 359
BstKTI GATC 5 cut(s) 409, 436, 545, 709, 1250
BstMAI GTCTC 6 cut(s) 9, 55, 241, 373, 827, 1267
BstMBI GATC 5 cut(s) 406, 433, 542, 706, 1247
BstMCI CGRYCG 1 cut(s) 389
BstMWI GCNNNNNNNGC 9 cut(s) 155, 365, 445, 507, 680, 773, 815, 1012, 1021
BstNI CCWGG 3 cut(s) 454, 496, 867
BstNSI RCATGY 1 cut(s) 1198
BstPAI GACNNNNGTC 1 cut(s) 385
BstSCI CCNGG 5 cut(s) 452, 494, 865, 968, 1015
BstSFI CTRYAG 2 cut(s) 508, 566
BstUI CGCG 1 cut(s) 359
BstV1I GCAGC 2 cut(s) 229, 580
BstV2I GAAGAC 1 cut(s) 57
BstX2I RGATCY 1 cut(s) 542
BstXI CCANNNNNNTGG 1 cut(s) 502
BstYI RGATCY 1 cut(s) 542
BsuRI GGCC 6 cut(s) 198, 302, 368, 448, 452, 1006
BtgI CCRYGG 1 cut(s) 799
BtsCI GGATG 2 cut(s) 658, 769
BtsI GCAGTG 2 cut(s) 563, 711
BtsIMutI CAGTG 4 cut(s) 538, 563, 711, 736
Cac8I GCNNGC 3 cut(s) 196, 450, 1008
Cfr10I RCCGGY 7 cut(s) 162, 194, 224, 384, 441, 448, 843
Cfr13I GGNCC 6 cut(s) 99, 160, 186, 863, 995, 1115
CpoI CGGWCCG 1 cut(s) 160
CseI GACGC 3 cut(s) 51, 390, 588
Csp6I GTAC 1 cut(s) 1092
CspAI ACCGGT 1 cut(s) 441
CspI CGGWCCG 1 cut(s) 160
CviAII CATG 5 cut(s) 502, 577, 686, 1195, 1264
CviQI GTAC 1 cut(s) 1092
DdeI CTNAG 9 cut(s) 138, 327, 375, 414, 423, 530, 639, 1133, 1199
DpnI GATC 5 cut(s) 408, 435, 544, 708, 1249
DpnII GATC 5 cut(s) 406, 433, 542, 706, 1247
EaeI YGGCCR 2 cut(s) 446, 450
Eam1104I CTCTTC 1 cut(s) 589
EarI CTCTTC 1 cut(s) 589
EciI GGCGGA 2 cut(s) 173, 181
Ecl136II GAGCTC 1 cut(s) 471
Eco130I CCWWGG 1 cut(s) 292
Eco147I AGGCCT 2 cut(s) 302, 1006
Eco24I GRGCYC 2 cut(s) 151, 473
Eco31I GGTCTC 1 cut(s) 55
Eco47I GGWCC 6 cut(s) 99, 160, 186, 863, 995, 1115
Eco53kI GAGCTC 1 cut(s) 471
Eco57I CTGAAG 1 cut(s) 735
Eco88I CYCGRG 3 cut(s) 203, 210, 472
EcoICRI GAGCTC 1 cut(s) 471
EcoNI CCTNNNNNAGG 3 cut(s) 203, 399, 1099
EcoO109I RGGNCCY 1 cut(s) 863
EcoRI GAATTC 1 cut(s) 418
EcoRII CCWGG 3 cut(s) 452, 494, 865
EcoT14I CCWWGG 1 cut(s) 292
EcoT38I GRGCYC 2 cut(s) 151, 473
ErhI CCWWGG 1 cut(s) 292
Esp3I CGTCTC 2 cut(s) 373, 1267
FaeI CATG 5 cut(s) 505, 580, 689, 1198, 1267
FaiI YATR 9 cut(s) 104, 503, 578, 687, 1062, 1196, 1212, 1265, 1291
FalI AAGNNNNNCTT 2 cut(s) 586, 618
FaqI GGGAC 4 cut(s) 364, 493, 935, 1008
FatI CATG 5 cut(s) 501, 576, 685, 1194, 1263
FbaI TGATCA 1 cut(s) 433
FblI GTMKAC 1 cut(s) 389
Fnu4HI GCNGC 4 cut(s) 167, 243, 357, 569
FokI GGATG 2 cut(s) 665, 776
FriOI GRGCYC 2 cut(s) 151, 473
FseI GGCCGGCC 1 cut(s) 452
Fsp4HI GCNGC 4 cut(s) 167, 243, 357, 569
FspBI CTAG 5 cut(s) 459, 657, 897, 990, 1002
GluI GCNGC 4 cut(s) 167, 243, 357, 569
GsuI CTGGAG 1 cut(s) 318
HaeIII GGCC 6 cut(s) 198, 302, 368, 448, 452, 1006
HgaI GACGC 3 cut(s) 51, 390, 588
Hin1I GRCGYC 1 cut(s) 382
Hin1II CATG 5 cut(s) 505, 580, 689, 1198, 1267
HincII GTYRAC 1 cut(s) 390
HindII GTYRAC 1 cut(s) 390
HindIII AAGCTT 1 cut(s) 1022
HinfI GANTC 2 cut(s) 331, 689
HphI GGTGA 4 cut(s) 226, 308, 956, 1038
Hpy166II GTNNAC 1 cut(s) 390
Hpy188III TCNNGA 2 cut(s) 69, 897
Hpy8I GTNNAC 1 cut(s) 390
HpyAV CCTTC 7 cut(s) 18, 24, 358, 643, 1077, 1134, 1270
HpyCH4III ACNGT 4 cut(s) 49, 465, 731, 1217
HpyF10VI GCNNNNNNNGC 9 cut(s) 155, 365, 445, 507, 680, 773, 815, 1012, 1021
HpyF3I CTNAG 9 cut(s) 138, 327, 375, 414, 423, 530, 639, 1133, 1199
Hsp92I GRCGYC 1 cut(s) 382
Hsp92II CATG 5 cut(s) 505, 580, 689, 1198, 1267
KroI GCCGGC 2 cut(s) 194, 448
KroNI GCCGGC 2 cut(s) 196, 450
Ksp22I TGATCA 1 cut(s) 433
Kzo9I GATC 5 cut(s) 406, 433, 542, 706, 1247
LguI GCTCTTC 1 cut(s) 589
LmnI GCTCC 2 cut(s) 273, 498
Lsp1109I GCAGC 2 cut(s) 229, 580
LweI GCATC 6 cut(s) 65, 643, 661, 754, 1177, 1243
MaeI CTAG 5 cut(s) 459, 657, 897, 990, 1002
MaeIII GTNAC 2 cut(s) 259, 1044
MalI GATC 5 cut(s) 408, 435, 544, 708, 1249
MboI GATC 5 cut(s) 406, 433, 542, 706, 1247
MflI RGATCY 1 cut(s) 542
MhlI GDGCHC 3 cut(s) 151, 473, 565
MluCI AATT 3 cut(s) 418, 676, 911
MlyI GAGTC 1 cut(s) 325
MroNI GCCGGC 2 cut(s) 194, 448
MseI TTAA 1 cut(s) 939
MslI CAYNNNNRTG 1 cut(s) 809
MspR9I CCNGG 5 cut(s) 454, 496, 867, 970, 1017
Mva1269I GAATGC 1 cut(s) 640
MvaI CCWGG 3 cut(s) 454, 496, 867
MvnI CGCG 1 cut(s) 359
MwoI GCNNNNNNNGC 9 cut(s) 155, 365, 445, 507, 680, 773, 815, 1012, 1021
NaeI GCCGGC 2 cut(s) 196, 450
NciI CCSGG 2 cut(s) 970, 1017
NdeII GATC 5 cut(s) 406, 433, 542, 706, 1247
NgoMIV GCCGGC 2 cut(s) 194, 448
NlaIII CATG 5 cut(s) 505, 580, 689, 1198, 1267
NlaIV GGNNCC 4 cut(s) 229, 775, 864, 996
NmeAIII GCCGAG 3 cut(s) 89, 229, 369
NmuCI GTSAC 2 cut(s) 259, 1044
NspI RCATGY 1 cut(s) 1198
NspV TTCGAA 1 cut(s) 116
PceI AGGCCT 2 cut(s) 302, 1006
PciSI GCTCTTC 1 cut(s) 589
PcsI WCGNNNNNNNCGW 1 cut(s) 59
PctI GAATGC 1 cut(s) 640
PdiI GCCGGC 2 cut(s) 196, 450
PfeI GAWTC 1 cut(s) 689
PfoI TCCNGGA 1 cut(s) 494
PinAI ACCGGT 1 cut(s) 441
PkrI GCNGC 4 cut(s) 168, 244, 358, 570
PleI GAGTC 1 cut(s) 325
PpsI GAGTC 1 cut(s) 325
PpuMI RGGWCCY 1 cut(s) 863
PshAI GACNNNNGTC 1 cut(s) 385
Psp124BI GAGCTC 1 cut(s) 473
Psp5II RGGWCCY 1 cut(s) 863
Psp6I CCWGG 3 cut(s) 452, 494, 865
PspGI CCWGG 3 cut(s) 452, 494, 865
PspN4I GGNNCC 4 cut(s) 229, 775, 864, 996
PspPI GGNCC 6 cut(s) 99, 160, 186, 863, 995, 1115
PspPPI RGGWCCY 1 cut(s) 863
PstI CTGCAG 2 cut(s) 512, 570
PsuI RGATCY 1 cut(s) 542
RigI GGCCGGCC 1 cut(s) 452
RsaI GTAC 1 cut(s) 1093
RsaNI GTAC 1 cut(s) 1092
RseI CAYNNNNRTG 1 cut(s) 809
Rsr2I CGGWCCG 1 cut(s) 160
RsrII CGGWCCG 1 cut(s) 160
SacI GAGCTC 1 cut(s) 473
SalI GTCGAC 1 cut(s) 388
SapI GCTCTTC 1 cut(s) 589
SaqAI TTAA 1 cut(s) 939
SatI GCNGC 4 cut(s) 167, 243, 357, 569
Sau3AI GATC 5 cut(s) 406, 433, 542, 706, 1247
Sau96I GGNCC 6 cut(s) 99, 160, 186, 863, 995, 1115
SchI GAGTC 1 cut(s) 325
ScrFI CCNGG 5 cut(s) 454, 496, 867, 970, 1017
SduI GDGCHC 3 cut(s) 151, 473, 565
SfaNI GCATC 6 cut(s) 65, 643, 661, 754, 1177, 1243
SfcI CTRYAG 2 cut(s) 508, 566
SfuI TTCGAA 1 cut(s) 116
SgrAI CRCCGGYG 1 cut(s) 441
SinI GGWCC 6 cut(s) 99, 160, 186, 863, 995, 1115
SmiMI CAYNNNNRTG 1 cut(s) 809
SmlI CTYRAG 1 cut(s) 94
SmoI CTYRAG 1 cut(s) 94
Sse9I AATT 3 cut(s) 418, 676, 911
SseBI AGGCCT 2 cut(s) 302, 1006
SsiI CCGC 7 cut(s) 158, 166, 192, 357, 809, 853, 1231
SspMI CTAG 5 cut(s) 459, 657, 897, 990, 1002
SstI GAGCTC 1 cut(s) 473
StuI AGGCCT 2 cut(s) 302, 1006
StyD4I CCNGG 5 cut(s) 452, 494, 865, 968, 1015
StyI CCWWGG 1 cut(s) 292
TaaI ACNGT 4 cut(s) 49, 465, 731, 1217
TaqI TCGA 9 cut(s) 26, 32, 116, 122, 389, 559, 606, 787, 929
TasI AATT 3 cut(s) 418, 676, 911
TauI GCSGC 2 cut(s) 169, 359
TfiI GAWTC 1 cut(s) 689
Tru1I TTAA 1 cut(s) 939
Tru9I TTAA 1 cut(s) 939
TscAI CASTG 4 cut(s) 538, 570, 718, 736
TseFI GTSAC 2 cut(s) 259, 1044
TseI GCWGC 2 cut(s) 242, 568
Tsp45I GTSAC 2 cut(s) 259, 1044
TspDTI ATGAA 3 cut(s) 702, 1026, 1252
TspGWI ACGGA 1 cut(s) 75
TspRI CASTG 4 cut(s) 538, 570, 718, 736
VpaK11BI GGWCC 6 cut(s) 99, 160, 186, 863, 995, 1115
XagI CCTNNNNNAGG 3 cut(s) 203, 399, 1099
XapI RAATTY 2 cut(s) 418, 911
XbaI TCTAGA 1 cut(s) 896
XceI RCATGY 1 cut(s) 1198
XmiI GTMKAC 1 cut(s) 389
XspI CTAG 5 cut(s) 459, 657, 897, 990, 1002
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.