Rh6BG052700

QWRF motif-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0005693
Physical Location & Seq
Forward (+)
8657137 .. 8664681
7545 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG052700.1

Sequence Viewer

Length: 1968 bp
ATGGTGGCTGCCATTTCCGACCCGACCCATCTCCAGACCCAATCTCGGCCGCCGCTTCTTCCCTCAGACAATGCCTCCTCCGCCGTTCAACGACGCGTCGTCCTCCGCGGCAGGCAGGTCTCCTCCAAGTACATGTCCACTCCTTCTCCTTCTTCTTCAAACTCGACTTCTACCTCTACTACAGTCTCCTCCTCCGCTTCTTCCAGGAGATGCCCTTCGCCGCTGCTCTCGCGGTCGATGAACTCGTCTCCGGCGCCGAATTCGGGCCCGAAGCGGGCCCAATCGGCCGACCGGAGGCGACCCGGTACGTCCCGGGCCCTACCGGAGCACCGGCTGAGCAATGCGGGTTCCGAAGTCTCCGCCGCCACCAGGCTCCTCGTCACCTCCACCCGGAGCCTCTCGGTTTCGTTTCAGGGCGAGGCGTTCTCGCTGCCGATTAGTAAGACCAAGGCGGCGGCAGGGGCTGCTCCGGCGAGGAAGGCCACGCCGGAGCGGAGGAGGTCGACGCCGGTGAGAAGAGAAGGAGATCAGGTGGAGAATTCTAGGCCCTCCGGCGGTGGTGATCAGCACCGGTGGCCGGCCCGGAGTCGGCAACCGAATTCGAATTCGGGTTATTCGAACGCGAATTTGAGTAGGAGTATGGACTGTAGTAGTGTGGAGAAGAAGAGTGTGAGTGTGAATGGGGTCGGATCTGGTGTGGTAGCTAGGGCGTTGCAGCAGTCTATGATTGAGGAGAGTAGAGGATCGAGGTCAAGATCGTCTTTTGATGGTAGGTTGAGCTTGGATTTGGGGCATTTTGGGGGCAATACTGAGGTTTTGAGGGGTGCTCAGCACAACCCAGATGCTAATAATGAGTCTTCTGTGCCGTCTGATCTCACTGCTTCCGATACAGACAGTGTTTCTTCGGGGAGCACTTCGGGTGTTCAGGACTCCAACAATGTGGCTTCTGCCAAGGCCGCACGAGTGGCCGCCCCTCGCGGCATTGCTGTGTCTGCAAGGTTCTGGCAGGAGACGAATAGCAGGTTGCGGAGGTTGCAGGATCCCGGTTCACCTTTGGCGACTAGTCCTGTGTCGAGAGCAGGGGCCGCGGGGAAGTATATGCAGGCGAAAAAGTTGAATGGGGAGAATCCTGTGTCCTCTCCGAGGACGGTGGCTTCTCCCATTAGAGGAGCTACTAGGCCGGCTTCCCCGGGGAAGCTGTGGACGTCTTCGTCTTTGTCACCTTCGAGGGGGAATGCTAGTCCTTCAAGGGTGAGGAATAGTTTTAGTGGTCCATTGAGTAGTGGTTATGCTGCTAGTACGCCTTCGTTTCTTAGTTTCTCTATTGATACCCGGAGAGGGAAGAAGGGGGAAGACCGCATTGTTGATGCACACATGCTCAGGCTTCTGTATAACCGTTATCTGCAGTGGCGGTTTGTAAATGCAAGAGCAGATGCTACATACATGGTGCAGAGAGTGAATGCAGAGGAAAGTCTTTGGAATGCATGGGTAACTATCTCAGAGCTACGACATAATGTCACACTTAAAAGGATCAAGTTACTTTTGTTGAGGCATAAGTTGAAGCTAACTTCTATCCTCAAAGGACAAATTACTTATTTGGAAGAATGGGCTCTTCTGGACAGAGATCACTCTAGTTCTTTGCTAGGAGCGAATGAAGCTTTGCAAGCCAGTACCCTTCGTCTTCCAGTCGTTGGAAAAGCAATAGTTGACTTTCAAAAGCTGAAAGATTCTGTGGGCTCAGCAGCCGATGTGATGCAGGCAATGGCATCCTCGATATGCTCGCTTTCATCAAAGGTGGAAGAAATGAATTCCTTGGTATGTGAACTTGTAAAGGTGACTACAACAGAACGTCTTGTGCTTGAACAATGCAAAGATTTTTTGTCAACATTATCAGCCATGCAAGTCAAGGACTGTAGCTTGAGAACACATATAATACAACTTAAACGTCTACCATTACCCGCAGCCTGA

Protein Analysis

655

Amino Acids

70.35

Weight (kDa)

10.7

Isoelectric Point (pI)

63.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
QWRF PF04484 299 - 621 6.8e-113 QWRF family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1210
AatII GACGTC 1 cut(s) 1208
Acc36I ACCTGC 2 cut(s) 106, 1011
AccB1I GGYRCC 1 cut(s) 253
AccB7I CCANNNNNTGG 1 cut(s) 1691
AccBSI CCGCTC 1 cut(s) 493
AccI GTMKAC 2 cut(s) 503, 1948
AccII CGCG 6 cut(s) 96, 108, 232, 623, 978, 1088
AclWI GGATC 5 cut(s) 697, 751, 1034, 1047, 1538
AcoI YGGCCR 4 cut(s) 47, 285, 575, 966
AcsI RAATTY 6 cut(s) 259, 538, 598, 604, 625, 1807
AcyI GRCGYC 3 cut(s) 254, 506, 1205
AfaI GTAC 4 cut(s) 131, 307, 1300, 1672
AflIII ACRYGT 2 cut(s) 94, 132
AgeI ACCGGT 1 cut(s) 570
AgsI TTSAA 7 cut(s) 89, 159, 1117, 1248, 1561, 1715, 1862
AhdI GACNNNNNGTC 2 cut(s) 98, 1514
AhlI ACTAGT 1 cut(s) 1061
AjnI CCWGG 2 cut(s) 203, 368
AluBI AGCT 9 cut(s) 704, 780, 1172, 1198, 1504, 1564, 1658, 1720, 1917
AluI AGCT 9 cut(s) 704, 780, 1172, 1198, 1504, 1564, 1658, 1720, 1917
Alw21I GWGCWC 3 cut(s) 330, 829, 914
Alw26I GTCTC 5 cut(s) 124, 190, 252, 361, 1004
AlwI GGATC 5 cut(s) 697, 751, 1034, 1047, 1538
AlwNI CAGNNNCTG 1 cut(s) 464
Ama87I CYCGRG 2 cut(s) 312, 1189
ApaI GGGCCC 3 cut(s) 269, 280, 319
ApeKI GCWGC 8 cut(s) 8, 223, 430, 464, 715, 1292, 1742, 1961
ApoI RAATTY 6 cut(s) 259, 538, 598, 604, 625, 1807
AsiGI ACCGGT 1 cut(s) 570
AspLEI GCGC 1 cut(s) 256
AsuC2I CCSGG 9 cut(s) 303, 313, 314, 391, 583, 1044, 1190, 1191, 1333
AsuHPI GGTGA 7 cut(s) 373, 523, 572, 1041, 1212, 1264, 1846
AsuII TTCGAA 2 cut(s) 602, 617
AvaI CYCGRG 2 cut(s) 312, 1189
AvaII GGWCC 1 cut(s) 1271
BaeGI GKGCMC 3 cut(s) 269, 280, 319
BamHI GGATCC 1 cut(s) 1039
BanI GGYRCC 1 cut(s) 253
BanII GRGCYC 5 cut(s) 269, 280, 319, 1612, 1739
BauI CACGAG 1 cut(s) 960
BbsI GAAGAC 4 cut(s) 849, 1200, 1359, 1673
Bbv12I GWGCWC 3 cut(s) 330, 829, 914
BbvI GCAGC 6 cut(s) 210, 417, 451, 727, 1279, 1754
BccI CCATC 2 cut(s) 36, 761
BceAI ACGGC 2 cut(s) 68, 850
BciT130I CCWGG 2 cut(s) 205, 370
BclI TGATCA 1 cut(s) 562
BcnI CCSGG 9 cut(s) 303, 313, 314, 391, 583, 1044, 1190, 1191, 1333
BcoDI GTCTC 5 cut(s) 124, 190, 252, 361, 1004
BcuI ACTAGT 1 cut(s) 1061
BfaI CTAG 8 cut(s) 543, 705, 1062, 1176, 1239, 1296, 1632, 1643
BfmI CTRYAG 4 cut(s) 180, 646, 1403, 1912
BfoI RGCGCY 1 cut(s) 257
BfuAI ACCTGC 2 cut(s) 106, 1011
BglI GCCNNNNNGGC 1 cut(s) 284
BlpI GCTNAGC 3 cut(s) 335, 828, 1738
Bme18I GGWCC 1 cut(s) 1271
BmeRI GACNNNNNGTC 2 cut(s) 98, 1514
BmeT110I CYCGRG 2 cut(s) 312, 1189
BmiI GGNNCC 9 cut(s) 255, 267, 278, 317, 349, 374, 395, 1041, 1084
BmsI GCATC 6 cut(s) 200, 832, 1357, 1423, 1743, 1775
BpiI GAAGAC 4 cut(s) 849, 1200, 1359, 1673
BplI GAGNNNNNCTC 4 cut(s) 410, 442, 811, 843
BpmI CTGGAG 1 cut(s) 17
Bpu10I CCTNAGC 1 cut(s) 1379
Bpu1102I GCTNAGC 3 cut(s) 335, 828, 1738
Bpu14I TTCGAA 2 cut(s) 602, 617
BpuEI CTTGAG 1 cut(s) 1939
BpuMI CCSGG 9 cut(s) 303, 313, 314, 391, 583, 1044, 1190, 1191, 1333
BsaHI GRCGYC 3 cut(s) 254, 506, 1205
BsaI GGTCTC 1 cut(s) 124
BsaWI WCCGGW 3 cut(s) 291, 322, 570
BsaXI ACNNNNNCTCC 6 cut(s) 59, 89, 130, 160, 516, 546
Bse118I RCCGGY 5 cut(s) 330, 508, 570, 577, 1180
Bse1I ACTGG 2 cut(s) 1668, 1685
Bse3DI GCAATG 3 cut(s) 346, 981, 1767
BseBI CCWGG 2 cut(s) 205, 370
BseGI GGATG 1 cut(s) 1766
BseMI GCAATG 3 cut(s) 346, 981, 1767
BseMII CTCAG 7 cut(s) 78, 326, 801, 842, 1393, 1512, 1752
BseNI ACTGG 2 cut(s) 1668, 1685
BseRI GAGGAG 8 cut(s) 67, 112, 178, 181, 365, 511, 746, 1182
BseSI GKGCMC 3 cut(s) 269, 280, 319
BseX3I CGGCCG 2 cut(s) 47, 285
BseXI GCAGC 6 cut(s) 210, 417, 451, 727, 1279, 1754
BsgI GTGCAG 1 cut(s) 1469
Bsh1236I CGCG 6 cut(s) 96, 108, 232, 623, 978, 1088
Bsh1285I CGRYCG 4 cut(s) 50, 236, 288, 292
BshNI GGYRCC 1 cut(s) 253
BshTI ACCGGT 1 cut(s) 570
BsiEI CGRYCG 4 cut(s) 50, 236, 288, 292
BsiHKAI GWGCWC 3 cut(s) 330, 829, 914
BsiHKCI CYCGRG 2 cut(s) 312, 1189
BslFI GGGAC 1 cut(s) 295
BsmAI GTCTC 5 cut(s) 124, 190, 252, 361, 1004
BsmBI CGTCTC 2 cut(s) 252, 1004
BsmFI GGGAC 1 cut(s) 295
BsmI GAATGC 3 cut(s) 1240, 1465, 1486
Bso31I GGTCTC 1 cut(s) 124
BsoBI CYCGRG 2 cut(s) 312, 1189
Bsp119I TTCGAA 2 cut(s) 602, 617
Bsp120I GGGCCC 3 cut(s) 265, 276, 315
Bsp1286I GDGCHC 8 cut(s) 269, 280, 319, 330, 829, 914, 1612, 1739
Bsp143I GATC 9 cut(s) 526, 562, 689, 743, 755, 871, 1039, 1530, 1624
Bsp1720I GCTNAGC 3 cut(s) 335, 828, 1738
BspCNI CTCAG 7 cut(s) 77, 327, 802, 841, 1392, 1511, 1751
BspFNI CGCG 6 cut(s) 96, 108, 232, 623, 978, 1088
BspLI GGNNCC 9 cut(s) 255, 267, 278, 317, 349, 374, 395, 1041, 1084
BspMAI CTGCAG 1 cut(s) 1407
BspMI ACCTGC 2 cut(s) 106, 1011
BspPI GGATC 5 cut(s) 697, 751, 1034, 1047, 1538
BspQI GCTCTTC 1 cut(s) 1617
BspT104I TTCGAA 2 cut(s) 602, 617
BspT107I GGYRCC 1 cut(s) 253
BspTNI GGTCTC 1 cut(s) 124
BsrBI CCGCTC 1 cut(s) 493
BsrDI GCAATG 3 cut(s) 346, 981, 1767
BsrFI RCCGGY 5 cut(s) 330, 508, 570, 577, 1180
BsrI ACTGG 2 cut(s) 1668, 1685
BssAI RCCGGY 5 cut(s) 330, 508, 570, 577, 1180
BssMI GATC 9 cut(s) 526, 562, 689, 743, 755, 871, 1039, 1530, 1624
BssNI GRCGYC 3 cut(s) 254, 506, 1205
BssSI CACGAG 1 cut(s) 960
BssT1I CCWWGG 3 cut(s) 447, 951, 1812
Bst2BI CACGAG 1 cut(s) 960
Bst2UI CCWGG 2 cut(s) 205, 370
Bst4CI ACNGT 6 cut(s) 184, 647, 896, 1150, 1397, 1913
Bst6I CTCTTC 3 cut(s) 511, 659, 1617
BstACI GRCGYC 3 cut(s) 254, 506, 1205
BstAPI GCANNNNNTGC 1 cut(s) 464
BstBI TTCGAA 2 cut(s) 602, 617
BstC8I GCNNGC 8 cut(s) 113, 276, 579, 1104, 1182, 1665, 1758, 1781
BstDEI CTNAG 8 cut(s) 64, 335, 810, 828, 1313, 1379, 1498, 1738
BstDSI CCRYGG 2 cut(s) 106, 1086
BstENI CCTNNNNNAGG 1 cut(s) 1141
BstF5I GGATG 1 cut(s) 1766
BstFNI CGCG 6 cut(s) 96, 108, 232, 623, 978, 1088
BstH2I RGCGCY 1 cut(s) 257
BstHHI GCGC 1 cut(s) 256
BstKTI GATC 9 cut(s) 529, 565, 692, 746, 758, 874, 1042, 1533, 1627
BstMAI GTCTC 5 cut(s) 124, 190, 252, 361, 1004
BstMBI GATC 9 cut(s) 526, 562, 689, 743, 755, 871, 1039, 1530, 1624
BstMCI CGRYCG 4 cut(s) 50, 236, 288, 292
BstNI CCWGG 2 cut(s) 205, 370
BstNSI RCATGY 2 cut(s) 136, 1378
BstSFI CTRYAG 4 cut(s) 180, 646, 1403, 1912
BstSLI GKGCMC 3 cut(s) 269, 280, 319
BstUI CGCG 6 cut(s) 96, 108, 232, 623, 978, 1088
BstV1I GCAGC 6 cut(s) 210, 417, 451, 727, 1279, 1754
BstV2I GAAGAC 4 cut(s) 849, 1200, 1359, 1673
BstX2I RGATCY 2 cut(s) 689, 1039
BstXI CCANNNNNNTGG 1 cut(s) 940
BstYI RGATCY 2 cut(s) 689, 1039
BstZI CGGCCG 2 cut(s) 47, 285
BtgI CCRYGG 2 cut(s) 106, 1086
BtsCI GGATG 1 cut(s) 1766
BtsI GCAGTG 2 cut(s) 876, 1412
BtsIMutI CAGTG 3 cut(s) 876, 901, 1412
BveI ACCTGC 2 cut(s) 106, 1011
Cac8I GCNNGC 8 cut(s) 113, 276, 579, 1104, 1182, 1665, 1758, 1781
CaiI CAGNNNCTG 1 cut(s) 464
CfoI GCGC 1 cut(s) 256
Cfr10I RCCGGY 5 cut(s) 330, 508, 570, 577, 1180
Cfr42I CCGCGG 2 cut(s) 109, 1089
Cfr9I CCCGGG 2 cut(s) 312, 1189
CseI GACGC 3 cut(s) 85, 102, 514
Csp6I GTAC 4 cut(s) 130, 306, 1299, 1671
CspAI ACCGGT 1 cut(s) 570
CviAII CATG 5 cut(s) 133, 1375, 1444, 1485, 1897
CviQI GTAC 4 cut(s) 130, 306, 1299, 1671
DdeI CTNAG 8 cut(s) 64, 335, 810, 828, 1313, 1379, 1498, 1738
DinI GGCGCC 1 cut(s) 255
DpnI GATC 9 cut(s) 528, 564, 691, 745, 757, 873, 1041, 1532, 1626
DpnII GATC 9 cut(s) 526, 562, 689, 743, 755, 871, 1039, 1530, 1624
DrdI GACNNNNNNGTC 1 cut(s) 1210
DriI GACNNNNNGTC 2 cut(s) 98, 1514
DseDI GACNNNNNNGTC 1 cut(s) 1210
EaeI YGGCCR 4 cut(s) 47, 285, 575, 966
EagI CGGCCG 2 cut(s) 47, 285
Eam1104I CTCTTC 3 cut(s) 511, 659, 1617
Eam1105I GACNNNNNGTC 2 cut(s) 98, 1514
EarI CTCTTC 3 cut(s) 511, 659, 1617
EciI GGCGGA 2 cut(s) 70, 349
EclXI CGGCCG 2 cut(s) 47, 285
Eco130I CCWWGG 3 cut(s) 447, 951, 1812
Eco24I GRGCYC 5 cut(s) 269, 280, 319, 1612, 1739
Eco31I GGTCTC 1 cut(s) 124
Eco47I GGWCC 1 cut(s) 1271
Eco52I CGGCCG 2 cut(s) 47, 285
Eco88I CYCGRG 2 cut(s) 312, 1189
EcoNI CCTNNNNNAGG 1 cut(s) 1141
EcoO109I RGGNCCY 2 cut(s) 316, 546
EcoRI GAATTC 5 cut(s) 259, 538, 598, 604, 1807
EcoRII CCWGG 2 cut(s) 203, 368
EcoT14I CCWWGG 3 cut(s) 447, 951, 1812
EcoT22I ATGCAT 1 cut(s) 1486
EcoT38I GRGCYC 5 cut(s) 269, 280, 319, 1612, 1739
EgeI GGCGCC 1 cut(s) 255
EheI GGCGCC 1 cut(s) 255
ErhI CCWWGG 3 cut(s) 447, 951, 1812
Esp3I CGTCTC 2 cut(s) 252, 1004
FaeI CATG 5 cut(s) 136, 1378, 1447, 1488, 1900
FalI AAGNNNNNCTT 2 cut(s) 745, 777
FaqI GGGAC 1 cut(s) 295
FatI CATG 5 cut(s) 132, 1374, 1443, 1484, 1896
FauI CCCGC 4 cut(s) 267, 337, 1081, 1966
FbaI TGATCA 1 cut(s) 562
FblI GTMKAC 2 cut(s) 503, 1948
FokI GGATG 1 cut(s) 1753
FriOI GRGCYC 5 cut(s) 269, 280, 319, 1612, 1739
FseI GGCCGGCC 1 cut(s) 581
FspBI CTAG 8 cut(s) 543, 705, 1062, 1176, 1239, 1296, 1632, 1643
GlaI GCGC 1 cut(s) 255
GsuI CTGGAG 1 cut(s) 17
HaeII RGCGCY 1 cut(s) 257
HgaI GACGC 3 cut(s) 85, 102, 514
HhaI GCGC 1 cut(s) 256
Hin1I GRCGYC 3 cut(s) 254, 506, 1205
Hin1II CATG 5 cut(s) 136, 1378, 1447, 1488, 1900
Hin6I GCGC 1 cut(s) 254
HinP1I GCGC 1 cut(s) 254
HincII GTYRAC 3 cut(s) 504, 1708, 1884
HindII GTYRAC 3 cut(s) 504, 1708, 1884
HindIII AAGCTT 1 cut(s) 1656
HinfI GANTC 5 cut(s) 586, 854, 929, 1126, 1727
HphI GGTGA 7 cut(s) 373, 523, 572, 1041, 1212, 1264, 1846
Hpy166II GTNNAC 8 cut(s) 138, 504, 1049, 1203, 1708, 1823, 1884, 1949
Hpy188I TCNGA 8 cut(s) 19, 67, 352, 689, 871, 886, 1143, 1501
Hpy188III TCNNGA 5 cut(s) 34, 753, 926, 1074, 1616
Hpy8I GTNNAC 8 cut(s) 138, 504, 1049, 1203, 1708, 1823, 1884, 1949
Hpy99I CGWCG 3 cut(s) 96, 101, 508
HpyCH4III ACNGT 6 cut(s) 184, 647, 896, 1150, 1397, 1913
HpyCH4IV ACGT 4 cut(s) 308, 1205, 1849, 1945
HpyF3I CTNAG 8 cut(s) 64, 335, 810, 828, 1313, 1379, 1498, 1738
HpySE526I ACGT 4 cut(s) 308, 1205, 1849, 1945
Hsp92I GRCGYC 3 cut(s) 254, 506, 1205
Hsp92II CATG 5 cut(s) 136, 1378, 1447, 1488, 1900
HspAI GCGC 1 cut(s) 254
KasI GGCGCC 1 cut(s) 253
KroI GCCGGC 2 cut(s) 577, 1180
KroNI GCCGGC 2 cut(s) 579, 1182
Ksp22I TGATCA 1 cut(s) 562
KspI CCGCGG 2 cut(s) 109, 1089
Kzo9I GATC 9 cut(s) 526, 562, 689, 743, 755, 871, 1039, 1530, 1624
LguI GCTCTTC 1 cut(s) 1617
LmnI GCTCC 8 cut(s) 325, 378, 393, 472, 490, 909, 1169, 1646
Lsp1109I GCAGC 6 cut(s) 210, 417, 451, 727, 1279, 1754
LweI GCATC 6 cut(s) 200, 832, 1357, 1423, 1743, 1775
MaeI CTAG 8 cut(s) 543, 705, 1062, 1176, 1239, 1296, 1632, 1643
MaeII ACGT 4 cut(s) 308, 1205, 1849, 1945
MaeIII GTNAC 6 cut(s) 379, 1218, 1489, 1516, 1536, 1834
MalI GATC 9 cut(s) 528, 564, 691, 745, 757, 873, 1041, 1532, 1626
MbiI CCGCTC 1 cut(s) 493
MboI GATC 9 cut(s) 526, 562, 689, 743, 755, 871, 1039, 1530, 1624
MflI RGATCY 2 cut(s) 689, 1039
MhlI GDGCHC 8 cut(s) 269, 280, 319, 330, 829, 914, 1612, 1739
MluCI AATT 7 cut(s) 259, 538, 598, 604, 625, 1587, 1807
MluI ACGCGT 1 cut(s) 94
Mly113I GGCGCC 1 cut(s) 254
MlyI GAGTC 3 cut(s) 595, 863, 923
MmeI TCCRAC 4 cut(s) 42, 667, 957, 1672
Mph1103I ATGCAT 1 cut(s) 1486
MroNI GCCGGC 2 cut(s) 577, 1180
MseI TTAA 2 cut(s) 1524, 1941
MslI CAYNNNNRTG 1 cut(s) 986
MspA1I CMGCKG 3 cut(s) 108, 223, 1088
Mva1269I GAATGC 3 cut(s) 1240, 1465, 1486
MvaI CCWGG 2 cut(s) 205, 370
MvnI CGCG 6 cut(s) 96, 108, 232, 623, 978, 1088
NaeI GCCGGC 2 cut(s) 579, 1182
NarI GGCGCC 1 cut(s) 254
NciI CCSGG 9 cut(s) 303, 313, 314, 391, 583, 1044, 1190, 1191, 1333
NdeII GATC 9 cut(s) 526, 562, 689, 743, 755, 871, 1039, 1530, 1624
NgoMIV GCCGGC 2 cut(s) 577, 1180
NlaIII CATG 5 cut(s) 136, 1378, 1447, 1488, 1900
NlaIV GGNNCC 9 cut(s) 255, 267, 278, 317, 349, 374, 395, 1041, 1084
NmeAIII GCCGAG 1 cut(s) 25
NmuCI GTSAC 4 cut(s) 379, 1218, 1516, 1834
NsiI ATGCAT 1 cut(s) 1486
NspI RCATGY 2 cut(s) 136, 1378
NspV TTCGAA 2 cut(s) 602, 617
PciI ACATGT 1 cut(s) 132
PciSI GCTCTTC 1 cut(s) 1617
PcsI WCGNNNNNNNCGW 2 cut(s) 242, 614
PctI GAATGC 3 cut(s) 1240, 1465, 1486
PdiI GCCGGC 2 cut(s) 579, 1182
PfeI GAWTC 2 cut(s) 1126, 1727
PflMI CCANNNNNTGG 1 cut(s) 1691
PfoI TCCNGGA 1 cut(s) 203
PinAI ACCGGT 1 cut(s) 570
PleI GAGTC 3 cut(s) 594, 862, 923
PluTI GGCGCC 1 cut(s) 257
PpsI GAGTC 3 cut(s) 594, 862, 923
PscI ACATGT 1 cut(s) 132
Psp6I CCWGG 2 cut(s) 203, 368
PspGI CCWGG 2 cut(s) 203, 368
PspN4I GGNNCC 9 cut(s) 255, 267, 278, 317, 349, 374, 395, 1041, 1084
PspOMI GGGCCC 3 cut(s) 265, 276, 315
PstI CTGCAG 1 cut(s) 1407
PstNI CAGNNNCTG 1 cut(s) 464
PsuI RGATCY 2 cut(s) 689, 1039
RigI GGCCGGCC 1 cut(s) 581
RsaI GTAC 4 cut(s) 131, 307, 1300, 1672
RsaNI GTAC 4 cut(s) 130, 306, 1299, 1671
RseI CAYNNNNRTG 1 cut(s) 986
SacII CCGCGG 2 cut(s) 109, 1089
SalI GTCGAC 1 cut(s) 502
SapI GCTCTTC 1 cut(s) 1617
SaqAI TTAA 2 cut(s) 1524, 1941
Sau3AI GATC 9 cut(s) 526, 562, 689, 743, 755, 871, 1039, 1530, 1624
SchI GAGTC 3 cut(s) 595, 863, 923
SduI GDGCHC 8 cut(s) 269, 280, 319, 330, 829, 914, 1612, 1739
SfaNI GCATC 6 cut(s) 200, 832, 1357, 1423, 1743, 1775
SfcI CTRYAG 4 cut(s) 180, 646, 1403, 1912
SfiI GGCCNNNNNGGCC 1 cut(s) 284
SfoI GGCGCC 1 cut(s) 255
Sfr303I CCGCGG 2 cut(s) 109, 1089
SfuI TTCGAA 2 cut(s) 602, 617
SgrAI CRCCGGYG 2 cut(s) 508, 570
SgrBI CCGCGG 2 cut(s) 109, 1089
SinI GGWCC 1 cut(s) 1271
SmaI CCCGGG 2 cut(s) 314, 1191
SmiMI CAYNNNNRTG 1 cut(s) 986
SmlI CTYRAG 1 cut(s) 1918
SmoI CTYRAG 1 cut(s) 1918
SpeI ACTAGT 1 cut(s) 1061
Sse9I AATT 7 cut(s) 259, 538, 598, 604, 625, 1587, 1807
SspDI GGCGCC 1 cut(s) 253
SspMI CTAG 8 cut(s) 543, 705, 1062, 1176, 1239, 1296, 1632, 1643
StyI CCWWGG 3 cut(s) 447, 951, 1812
TaaI ACNGT 6 cut(s) 184, 647, 896, 1150, 1397, 1913
TaiI ACGT 4 cut(s) 311, 1208, 1852, 1948
TaqI TCGA 9 cut(s) 164, 236, 503, 602, 617, 746, 1073, 1226, 1772
TasI AATT 7 cut(s) 259, 538, 598, 604, 625, 1587, 1807
TatI WGTACW 1 cut(s) 129
TfiI GAWTC 2 cut(s) 1126, 1727
Tru1I TTAA 2 cut(s) 1524, 1941
Tru9I TTAA 2 cut(s) 1524, 1941
TscAI CASTG 3 cut(s) 883, 901, 1412
TseFI GTSAC 4 cut(s) 379, 1218, 1516, 1834
TseI GCWGC 8 cut(s) 8, 223, 430, 464, 715, 1292, 1742, 1961
Tsp45I GTSAC 4 cut(s) 379, 1218, 1516, 1834
TspDTI ATGAA 4 cut(s) 254, 1668, 1776, 1820
TspMI CCCGGG 2 cut(s) 312, 1189
TspRI CASTG 3 cut(s) 883, 901, 1412
Van91I CCANNNNNTGG 1 cut(s) 1691
VpaK11BI GGWCC 1 cut(s) 1271
XagI CCTNNNNNAGG 1 cut(s) 1141
XapI RAATTY 6 cut(s) 259, 538, 598, 604, 625, 1807
XceI RCATGY 2 cut(s) 136, 1378
XmaI CCCGGG 2 cut(s) 312, 1189
XmiI GTMKAC 2 cut(s) 503, 1948
XspI CTAG 8 cut(s) 543, 705, 1062, 1176, 1239, 1296, 1632, 1643
ZraI GACGTC 1 cut(s) 1206
Zsp2I ATGCAT 1 cut(s) 1486
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.