Rw0G004810

QWRF motif-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00185
OG0005693
Physical Location & Seq
Forward (+)
17555 .. 24356
6802 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G004810.1

Sequence Viewer

Length: 1944 bp
ATGGTGGCTGCCATTTCCGACCCGACCCATCTCCAGACCCAATCTCGGCCGCCGCTTCTTCCCTCAGACAATGCCTCCTCCGCCGCCGTTCAACGACGCGTCGTCCTCCGCGGCAGGCAGGTCTCCTCCAAGTACATGTCCACTCCTTCTCCTTCTTCTTCAAACTCGACTTCTACCTCTACTACAGTCTCCTCCTCCGCTTCTTCCAGGAGATGCCCTTCGCCGCTGCTCTCGCGGTCGATGAACTCGTCTCCGGCGCCGAATTCGGGCCCGAAGCGGGCCCAATCGGCCGACCGGAGGCGACCCGGTACGGCCCGGACCATACCGGAGCACCGGCTGAGCAATGCGGGTTCCGAAGTCTCCGCCGCCACCAGACTCCTCGTCACCTCCACCCGGAGCCTCTCGGTTTCGTTTCAGGGCGAGGCGTTCTCGCTGCCGATTAGTAAGACCAAGGCGGCGGCCGCGGCGGCAGGGGCTGCTCCGGCGAGGAAGGCCACGCCGGAGCGGCGGAGGTCGACGCCGGTGAGAAGAGAAGGAGATCAGGTGGAGAATTCTAGGCCCTCCGGCGGTGGTGATCAGCACCGGTGGCCGGCCCGGAGTCGGCAACCGAATTCGAATTCGGGTTATTCGAACCCGAATTTGAGTAGGAGTATGGACTGTAGTAGTGTGGAGAAGAAGAGTGTGAATGTGAATGGGGTCGGATCTGGTGTGGTAGCTAGGGCGTTGCAGCAGTCTATGATTGAGGAGAGTAGAGGATCGAGGTCAAGATCGTCTTTTGATGGTAGGTTGAGCTTGGATTTGGGGCATTTTGGGGGCAATGCTGAGGTTTTGAGGGGTGCTCAGCACAACCCAGATGCTAATAATGAGTCTTCTGTGCCGTCTGATCTCACTGCTTCCGATACAGACAGTGTTTCTTCGGGGAGCACTTCGGGCCGCACGATGGCCGCCCCTCGCGGCATTGCTGTGTCTGCAAGGTTCTGGCAGGAGACGAATAGCAGGTTGCGGAGGTTGCAGGATCCCGGTTCACCTTTGGCGACTAGTCCTGTGTCGAGAGCAGGGGCAGCGGGGAAGTATATGCAGGCGAAAAAGTTGAATGGGGAGAATCCTGTGTCGTCTCCGAGGACGGTGGCTTCTCCCATTAGAGGAGCTACTAGGCCGGCTTCCCCGGGGAAGCTGTGGACGTCTTCGTCTTTGTCACCTTCGAGGGGGAATGCTAGTCCTTCAAGGGTGAGGAATAGTTTTAGTGGTCCATTGAGTAGTGGTTATGCTACTAGTACGCCTTCGTTTCTTAGTTTCTCTATTGATACCCGGAGAGGGAAGAAGGGGGAAGACCGCATTGTTGATGCACACATGCTCAGGCTTCTGTATAACCGTTATCTGCAGTGGCGGTTTGTAAATGCAAGAGCAGATGCTACATACATGGTGCAGAGAGTGAATGCAGAGGAAAATCTTTGGAATGCATGGGTAACTATCTCAGAGCTACGACATAATGTCACACTTAAAAGGATCAAGTTACTTTTGTTGAGGCATAAGTTGAAGCTAACTTCTATCCTCAAAGGACAAATTAGTTATTTGGAAGAATGGGCTCTTCTGGACAGAGATCACTCTAGTTCTTTGCTAGGAGCGAATGAAGCTTTGCAAGCCAGTACCCTTCGTCTTCCAGTCGTTGGAAAAGCAATAGTTGACTTTCAAAAGCTGAAAGATTCTGTGGGCTCTGCAGCTGATGTGATGCAGGCAATGGCATCCTCGATATGCTTGCTTTCATCAAAGGTGGAAGAAATGAATTCCTTGGTATCTGAACTTGTGAAGGTGACTACAACAGAACGTCTTGTGCTTGAACAATGCAAAGATTTTTTGTCAACGTTATCAGCCATGCAAGTCAAGGACTGTAGCTTGAGAACACATATAATACAACTAAAACGTCTACCATTACCCGCAGCCTGA

Protein Analysis

647

Amino Acids

69.6

Weight (kDa)

10.8

Isoelectric Point (pI)

62.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
QWRF PF04484 303 - 613 8.1e-114 QWRF family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1186
AatII GACGTC 1 cut(s) 1184
Acc36I ACCTGC 2 cut(s) 109, 987
AccB1I GGYRCC 1 cut(s) 256
AccB7I CCANNNNNTGG 1 cut(s) 1667
AccBSI CCGCTC 1 cut(s) 505
AccI GTMKAC 2 cut(s) 515, 1924
AccII CGCG 5 cut(s) 99, 111, 235, 464, 954
AclI AACGTT 1 cut(s) 1862
AclWI GGATC 5 cut(s) 709, 763, 1010, 1023, 1514
AcoI YGGCCR 5 cut(s) 47, 288, 459, 587, 942
AcsI RAATTY 6 cut(s) 262, 550, 610, 616, 637, 1783
AcyI GRCGYC 3 cut(s) 257, 518, 1181
AfaI GTAC 4 cut(s) 134, 310, 1276, 1648
AflIII ACRYGT 2 cut(s) 97, 135
AgeI ACCGGT 1 cut(s) 582
AgsI TTSAA 7 cut(s) 92, 162, 1093, 1224, 1537, 1691, 1838
AhdI GACNNNNNGTC 3 cut(s) 101, 380, 1490
AhlI ACTAGT 2 cut(s) 1037, 1271
AjnI CCWGG 1 cut(s) 206
Alw21I GWGCWC 3 cut(s) 333, 841, 926
Alw26I GTCTC 6 cut(s) 127, 193, 255, 364, 980, 1119
AlwI GGATC 5 cut(s) 709, 763, 1010, 1023, 1514
AlwNI CAGNNNCTG 1 cut(s) 476
Ama87I CYCGRG 1 cut(s) 1165
ApaI GGGCCC 2 cut(s) 272, 283
ApeKI GCWGC 8 cut(s) 8, 226, 433, 476, 727, 1061, 1718, 1937
ApoI RAATTY 6 cut(s) 262, 550, 610, 616, 637, 1783
AsiGI ACCGGT 1 cut(s) 582
AspLEI GCGC 1 cut(s) 259
AsuC2I CCSGG 8 cut(s) 306, 316, 394, 595, 1020, 1166, 1167, 1309
AsuHPI GGTGA 7 cut(s) 376, 535, 584, 1017, 1188, 1240, 1822
AsuII TTCGAA 2 cut(s) 614, 629
AvaI CYCGRG 1 cut(s) 1165
AvaII GGWCC 2 cut(s) 318, 1247
BaeGI GKGCMC 2 cut(s) 272, 283
BamHI GGATCC 1 cut(s) 1015
BanI GGYRCC 1 cut(s) 256
BanII GRGCYC 4 cut(s) 272, 283, 1588, 1715
BbsI GAAGAC 4 cut(s) 861, 1176, 1335, 1649
Bbv12I GWGCWC 3 cut(s) 333, 841, 926
BbvCI CCTCAGC 1 cut(s) 822
BbvI GCAGC 6 cut(s) 213, 420, 463, 739, 1073, 1730
BccI CCATC 3 cut(s) 36, 773, 934
BceAI ACGGC 3 cut(s) 71, 327, 862
BcgI CGANNNNNNTGC 2 cut(s) 1738, 1772
BciT130I CCWGG 1 cut(s) 208
BclI TGATCA 1 cut(s) 574
BcnI CCSGG 8 cut(s) 306, 316, 394, 595, 1020, 1166, 1167, 1309
BcoDI GTCTC 6 cut(s) 127, 193, 255, 364, 980, 1119
BcuI ACTAGT 2 cut(s) 1037, 1271
BfaI CTAG 8 cut(s) 555, 717, 1038, 1152, 1215, 1272, 1608, 1619
BfmI CTRYAG 5 cut(s) 183, 658, 1379, 1716, 1888
BfoI RGCGCY 1 cut(s) 260
BfuAI ACCTGC 2 cut(s) 109, 987
BglI GCCNNNNNGGC 3 cut(s) 287, 467, 505
BlpI GCTNAGC 2 cut(s) 338, 840
Bme1390I CCNGG 9 cut(s) 208, 306, 316, 394, 595, 1020, 1166, 1167, 1309
Bme18I GGWCC 2 cut(s) 318, 1247
BmeRI GACNNNNNGTC 3 cut(s) 101, 380, 1490
BmeT110I CYCGRG 1 cut(s) 1165
BmiI GGNNCC 6 cut(s) 258, 270, 281, 352, 398, 1017
BmrFI CCNGG 9 cut(s) 208, 306, 316, 394, 595, 1020, 1166, 1167, 1309
BmsI GCATC 6 cut(s) 203, 844, 1333, 1399, 1719, 1751
BpiI GAAGAC 4 cut(s) 861, 1176, 1335, 1649
BplI GAGNNNNNCTC 4 cut(s) 413, 445, 823, 855
BpmI CTGGAG 1 cut(s) 17
Bpu10I CCTNAGC 2 cut(s) 822, 1355
Bpu1102I GCTNAGC 2 cut(s) 338, 840
Bpu14I TTCGAA 2 cut(s) 614, 629
BpuEI CTTGAG 1 cut(s) 1915
BpuMI CCSGG 8 cut(s) 306, 316, 394, 595, 1020, 1166, 1167, 1309
BsaHI GRCGYC 3 cut(s) 257, 518, 1181
BsaI GGTCTC 1 cut(s) 127
BsaJI CCNNGG 8 cut(s) 109, 450, 462, 1118, 1164, 1165, 1166, 1788
BsaWI WCCGGW 3 cut(s) 294, 325, 582
BsaXI ACNNNNNCTCC 6 cut(s) 59, 89, 133, 163, 528, 558
Bse118I RCCGGY 5 cut(s) 333, 520, 582, 589, 1156
Bse1I ACTGG 2 cut(s) 1644, 1661
Bse3DI GCAATG 4 cut(s) 349, 823, 957, 1743
BseBI CCWGG 1 cut(s) 208
BseDI CCNNGG 8 cut(s) 109, 450, 462, 1118, 1164, 1165, 1166, 1788
BseGI GGATG 1 cut(s) 1742
BseMI GCAATG 4 cut(s) 349, 823, 957, 1743
BseMII CTCAG 6 cut(s) 78, 329, 813, 854, 1369, 1488
BseNI ACTGG 2 cut(s) 1644, 1661
BseRI GAGGAG 7 cut(s) 67, 115, 181, 184, 368, 758, 1158
BseSI GKGCMC 2 cut(s) 272, 283
BseX3I CGGCCG 3 cut(s) 47, 288, 459
BseXI GCAGC 6 cut(s) 213, 420, 463, 739, 1073, 1730
BsgI GTGCAG 1 cut(s) 1445
Bsh1236I CGCG 5 cut(s) 99, 111, 235, 464, 954
Bsh1285I CGRYCG 5 cut(s) 50, 239, 291, 295, 462
BshNI GGYRCC 1 cut(s) 256
BshTI ACCGGT 1 cut(s) 582
BsiEI CGRYCG 5 cut(s) 50, 239, 291, 295, 462
BsiHKAI GWGCWC 3 cut(s) 333, 841, 926
BsiHKCI CYCGRG 1 cut(s) 1165
BsmAI GTCTC 6 cut(s) 127, 193, 255, 364, 980, 1119
BsmBI CGTCTC 3 cut(s) 255, 980, 1119
BsmI GAATGC 3 cut(s) 1216, 1441, 1462
Bso31I GGTCTC 1 cut(s) 127
BsoBI CYCGRG 1 cut(s) 1165
Bsp119I TTCGAA 2 cut(s) 614, 629
Bsp120I GGGCCC 2 cut(s) 268, 279
Bsp1286I GDGCHC 7 cut(s) 272, 283, 333, 841, 926, 1588, 1715
Bsp143I GATC 9 cut(s) 538, 574, 701, 755, 767, 883, 1015, 1506, 1600
Bsp1720I GCTNAGC 2 cut(s) 338, 840
BspCNI CTCAG 6 cut(s) 77, 330, 814, 853, 1368, 1487
BspFNI CGCG 5 cut(s) 99, 111, 235, 464, 954
BspLI GGNNCC 6 cut(s) 258, 270, 281, 352, 398, 1017
BspMAI CTGCAG 2 cut(s) 1383, 1720
BspMI ACCTGC 2 cut(s) 109, 987
BspPI GGATC 5 cut(s) 709, 763, 1010, 1023, 1514
BspQI GCTCTTC 1 cut(s) 1593
BspT104I TTCGAA 2 cut(s) 614, 629
BspT107I GGYRCC 1 cut(s) 256
BspTNI GGTCTC 1 cut(s) 127
BsrBI CCGCTC 1 cut(s) 505
BsrDI GCAATG 4 cut(s) 349, 823, 957, 1743
BsrFI RCCGGY 5 cut(s) 333, 520, 582, 589, 1156
BsrI ACTGG 2 cut(s) 1644, 1661
BssAI RCCGGY 5 cut(s) 333, 520, 582, 589, 1156
BssECI CCNNGG 8 cut(s) 109, 450, 462, 1118, 1164, 1165, 1166, 1788
BssMI GATC 9 cut(s) 538, 574, 701, 755, 767, 883, 1015, 1506, 1600
BssNI GRCGYC 3 cut(s) 257, 518, 1181
BssT1I CCWWGG 2 cut(s) 450, 1788
Bst2UI CCWGG 1 cut(s) 208
Bst4CI ACNGT 6 cut(s) 187, 659, 908, 1126, 1373, 1889
Bst6I CTCTTC 3 cut(s) 523, 671, 1593
BstACI GRCGYC 3 cut(s) 257, 518, 1181
BstAPI GCANNNNNTGC 1 cut(s) 476
BstBI TTCGAA 2 cut(s) 614, 629
BstC8I GCNNGC 8 cut(s) 116, 279, 591, 1080, 1158, 1641, 1734, 1757
BstDEI CTNAG 7 cut(s) 64, 338, 822, 840, 1289, 1355, 1474
BstDSI CCRYGG 2 cut(s) 109, 462
BstF5I GGATG 1 cut(s) 1742
BstFNI CGCG 5 cut(s) 99, 111, 235, 464, 954
BstH2I RGCGCY 1 cut(s) 260
BstHHI GCGC 1 cut(s) 259
BstKTI GATC 9 cut(s) 541, 577, 704, 758, 770, 886, 1018, 1509, 1603
BstMAI GTCTC 6 cut(s) 127, 193, 255, 364, 980, 1119
BstMBI GATC 9 cut(s) 538, 574, 701, 755, 767, 883, 1015, 1506, 1600
BstMCI CGRYCG 5 cut(s) 50, 239, 291, 295, 462
BstNI CCWGG 1 cut(s) 208
BstNSI RCATGY 2 cut(s) 139, 1354
BstSCI CCNGG 9 cut(s) 206, 304, 314, 392, 593, 1018, 1164, 1165, 1307
BstSFI CTRYAG 5 cut(s) 183, 658, 1379, 1716, 1888
BstSLI GKGCMC 2 cut(s) 272, 283
BstUI CGCG 5 cut(s) 99, 111, 235, 464, 954
BstV1I GCAGC 6 cut(s) 213, 420, 463, 739, 1073, 1730
BstV2I GAAGAC 4 cut(s) 861, 1176, 1335, 1649
BstX2I RGATCY 2 cut(s) 701, 1015
BstYI RGATCY 2 cut(s) 701, 1015
BstZI CGGCCG 3 cut(s) 47, 288, 459
BtgI CCRYGG 2 cut(s) 109, 462
BtsCI GGATG 1 cut(s) 1742
BtsI GCAGTG 2 cut(s) 888, 1388
BtsIMutI CAGTG 3 cut(s) 888, 913, 1388
BveI ACCTGC 2 cut(s) 109, 987
Cac8I GCNNGC 8 cut(s) 116, 279, 591, 1080, 1158, 1641, 1734, 1757
CaiI CAGNNNCTG 1 cut(s) 476
CciNI GCGGCCGC 1 cut(s) 459
CfoI GCGC 1 cut(s) 259
Cfr10I RCCGGY 5 cut(s) 333, 520, 582, 589, 1156
Cfr42I CCGCGG 2 cut(s) 112, 465
Cfr9I CCCGGG 1 cut(s) 1165
CseI GACGC 3 cut(s) 88, 105, 526
Csp6I GTAC 4 cut(s) 133, 309, 1275, 1647
CspAI ACCGGT 1 cut(s) 582
CviAII CATG 5 cut(s) 136, 1351, 1420, 1461, 1873
CviQI GTAC 4 cut(s) 133, 309, 1275, 1647
DdeI CTNAG 7 cut(s) 64, 338, 822, 840, 1289, 1355, 1474
DinI GGCGCC 1 cut(s) 258
DpnI GATC 9 cut(s) 540, 576, 703, 757, 769, 885, 1017, 1508, 1602
DpnII GATC 9 cut(s) 538, 574, 701, 755, 767, 883, 1015, 1506, 1600
DrdI GACNNNNNNGTC 1 cut(s) 1186
DriI GACNNNNNGTC 3 cut(s) 101, 380, 1490
DseDI GACNNNNNNGTC 1 cut(s) 1186
EaeI YGGCCR 5 cut(s) 47, 288, 459, 587, 942
EagI CGGCCG 3 cut(s) 47, 288, 459
Eam1104I CTCTTC 3 cut(s) 523, 671, 1593
Eam1105I GACNNNNNGTC 3 cut(s) 101, 380, 1490
EarI CTCTTC 3 cut(s) 523, 671, 1593
EciI GGCGGA 3 cut(s) 70, 352, 523
EclXI CGGCCG 3 cut(s) 47, 288, 459
Eco130I CCWWGG 2 cut(s) 450, 1788
Eco24I GRGCYC 4 cut(s) 272, 283, 1588, 1715
Eco31I GGTCTC 1 cut(s) 127
Eco47I GGWCC 2 cut(s) 318, 1247
Eco52I CGGCCG 3 cut(s) 47, 288, 459
Eco88I CYCGRG 1 cut(s) 1165
EcoO109I RGGNCCY 1 cut(s) 558
EcoRI GAATTC 5 cut(s) 262, 550, 610, 616, 1783
EcoRII CCWGG 1 cut(s) 206
EcoT14I CCWWGG 2 cut(s) 450, 1788
EcoT22I ATGCAT 1 cut(s) 1462
EcoT38I GRGCYC 4 cut(s) 272, 283, 1588, 1715
EgeI GGCGCC 1 cut(s) 258
EheI GGCGCC 1 cut(s) 258
ErhI CCWWGG 2 cut(s) 450, 1788
Esp3I CGTCTC 3 cut(s) 255, 980, 1119
FaeI CATG 5 cut(s) 139, 1354, 1423, 1464, 1876
FalI AAGNNNNNCTT 2 cut(s) 757, 789
FatI CATG 5 cut(s) 135, 1350, 1419, 1460, 1872
FauI CCCGC 4 cut(s) 270, 340, 1057, 1942
FbaI TGATCA 1 cut(s) 574
FblI GTMKAC 2 cut(s) 515, 1924
FokI GGATG 1 cut(s) 1729
FriOI GRGCYC 4 cut(s) 272, 283, 1588, 1715
FseI GGCCGGCC 1 cut(s) 593
FspBI CTAG 8 cut(s) 555, 717, 1038, 1152, 1215, 1272, 1608, 1619
GlaI GCGC 1 cut(s) 258
GsuI CTGGAG 1 cut(s) 17
HaeII RGCGCY 1 cut(s) 260
HgaI GACGC 3 cut(s) 88, 105, 526
HhaI GCGC 1 cut(s) 259
Hin1I GRCGYC 3 cut(s) 257, 518, 1181
Hin1II CATG 5 cut(s) 139, 1354, 1423, 1464, 1876
Hin6I GCGC 1 cut(s) 257
HinP1I GCGC 1 cut(s) 257
HincII GTYRAC 3 cut(s) 516, 1684, 1860
HindII GTYRAC 3 cut(s) 516, 1684, 1860
HindIII AAGCTT 1 cut(s) 1632
HinfI GANTC 5 cut(s) 375, 598, 866, 1102, 1703
HphI GGTGA 7 cut(s) 376, 535, 584, 1017, 1188, 1240, 1822
Hpy166II GTNNAC 7 cut(s) 141, 516, 1025, 1179, 1684, 1860, 1925
Hpy188I TCNGA 9 cut(s) 19, 67, 355, 701, 883, 898, 1119, 1477, 1798
Hpy188III TCNNGA 4 cut(s) 34, 765, 1050, 1592
Hpy8I GTNNAC 7 cut(s) 141, 516, 1025, 1179, 1684, 1860, 1925
Hpy99I CGWCG 3 cut(s) 99, 104, 520
HpyCH4III ACNGT 6 cut(s) 187, 659, 908, 1126, 1373, 1889
HpyCH4IV ACGT 4 cut(s) 1181, 1825, 1862, 1921
HpyF3I CTNAG 7 cut(s) 64, 338, 822, 840, 1289, 1355, 1474
HpySE526I ACGT 4 cut(s) 1181, 1825, 1862, 1921
Hsp92I GRCGYC 3 cut(s) 257, 518, 1181
Hsp92II CATG 5 cut(s) 139, 1354, 1423, 1464, 1876
HspAI GCGC 1 cut(s) 257
KasI GGCGCC 1 cut(s) 256
KroI GCCGGC 2 cut(s) 589, 1156
KroNI GCCGGC 2 cut(s) 591, 1158
Ksp22I TGATCA 1 cut(s) 574
KspI CCGCGG 2 cut(s) 112, 465
Kzo9I GATC 9 cut(s) 538, 574, 701, 755, 767, 883, 1015, 1506, 1600
LguI GCTCTTC 1 cut(s) 1593
LmnI GCTCC 7 cut(s) 328, 396, 484, 502, 921, 1145, 1622
Lsp1109I GCAGC 6 cut(s) 213, 420, 463, 739, 1073, 1730
LweI GCATC 6 cut(s) 203, 844, 1333, 1399, 1719, 1751
MaeI CTAG 8 cut(s) 555, 717, 1038, 1152, 1215, 1272, 1608, 1619
MaeII ACGT 4 cut(s) 1181, 1825, 1862, 1921
MaeIII GTNAC 6 cut(s) 382, 1194, 1465, 1492, 1512, 1810
MalI GATC 9 cut(s) 540, 576, 703, 757, 769, 885, 1017, 1508, 1602
MbiI CCGCTC 1 cut(s) 505
MboI GATC 9 cut(s) 538, 574, 701, 755, 767, 883, 1015, 1506, 1600
MflI RGATCY 2 cut(s) 701, 1015
MhlI GDGCHC 7 cut(s) 272, 283, 333, 841, 926, 1588, 1715
MluCI AATT 7 cut(s) 262, 550, 610, 616, 637, 1563, 1783
MluI ACGCGT 1 cut(s) 97
Mly113I GGCGCC 1 cut(s) 257
MlyI GAGTC 3 cut(s) 369, 607, 875
MmeI TCCRAC 3 cut(s) 42, 679, 1648
Mph1103I ATGCAT 1 cut(s) 1462
MroNI GCCGGC 2 cut(s) 589, 1156
MseI TTAA 1 cut(s) 1500
MslI CAYNNNNRTG 1 cut(s) 962
MspA1I CMGCKG 5 cut(s) 111, 226, 464, 1064, 1721
MspR9I CCNGG 9 cut(s) 208, 306, 316, 394, 595, 1020, 1166, 1167, 1309
Mva1269I GAATGC 3 cut(s) 1216, 1441, 1462
MvaI CCWGG 1 cut(s) 208
MvnI CGCG 5 cut(s) 99, 111, 235, 464, 954
NaeI GCCGGC 2 cut(s) 591, 1158
NarI GGCGCC 1 cut(s) 257
NciI CCSGG 8 cut(s) 306, 316, 394, 595, 1020, 1166, 1167, 1309
NdeII GATC 9 cut(s) 538, 574, 701, 755, 767, 883, 1015, 1506, 1600
NgoMIV GCCGGC 2 cut(s) 589, 1156
NlaIII CATG 5 cut(s) 139, 1354, 1423, 1464, 1876
NlaIV GGNNCC 6 cut(s) 258, 270, 281, 352, 398, 1017
NmeAIII GCCGAG 1 cut(s) 25
NmuCI GTSAC 4 cut(s) 382, 1194, 1492, 1810
NotI GCGGCCGC 1 cut(s) 459
NsiI ATGCAT 1 cut(s) 1462
NspI RCATGY 2 cut(s) 139, 1354
NspV TTCGAA 2 cut(s) 614, 629
PciI ACATGT 1 cut(s) 135
PciSI GCTCTTC 1 cut(s) 1593
PcsI WCGNNNNNNNCGW 3 cut(s) 245, 626, 935
PctI GAATGC 3 cut(s) 1216, 1441, 1462
PdiI GCCGGC 2 cut(s) 591, 1158
PfeI GAWTC 2 cut(s) 1102, 1703
PflMI CCANNNNNTGG 1 cut(s) 1667
PfoI TCCNGGA 1 cut(s) 206
PinAI ACCGGT 1 cut(s) 582
PleI GAGTC 3 cut(s) 369, 606, 874
PluTI GGCGCC 1 cut(s) 260
PpsI GAGTC 3 cut(s) 369, 606, 874
PscI ACATGT 1 cut(s) 135
Psp1406I AACGTT 1 cut(s) 1862
Psp6I CCWGG 1 cut(s) 206
PspGI CCWGG 1 cut(s) 206
PspN4I GGNNCC 6 cut(s) 258, 270, 281, 352, 398, 1017
PspOMI GGGCCC 2 cut(s) 268, 279
PstI CTGCAG 2 cut(s) 1383, 1720
PstNI CAGNNNCTG 1 cut(s) 476
PsuI RGATCY 2 cut(s) 701, 1015
PvuII CAGCTG 1 cut(s) 1721
RigI GGCCGGCC 1 cut(s) 593
RsaI GTAC 4 cut(s) 134, 310, 1276, 1648
RsaNI GTAC 4 cut(s) 133, 309, 1275, 1647
RseI CAYNNNNRTG 1 cut(s) 962
SacII CCGCGG 2 cut(s) 112, 465
SalI GTCGAC 1 cut(s) 514
SapI GCTCTTC 1 cut(s) 1593
SaqAI TTAA 1 cut(s) 1500
Sau3AI GATC 9 cut(s) 538, 574, 701, 755, 767, 883, 1015, 1506, 1600
SchI GAGTC 3 cut(s) 369, 607, 875
ScrFI CCNGG 9 cut(s) 208, 306, 316, 394, 595, 1020, 1166, 1167, 1309
SduI GDGCHC 7 cut(s) 272, 283, 333, 841, 926, 1588, 1715
SfaNI GCATC 6 cut(s) 203, 844, 1333, 1399, 1719, 1751
SfcI CTRYAG 5 cut(s) 183, 658, 1379, 1716, 1888
SfiI GGCCNNNNNGGCC 1 cut(s) 287
SfoI GGCGCC 1 cut(s) 258
Sfr303I CCGCGG 2 cut(s) 112, 465
SfuI TTCGAA 2 cut(s) 614, 629
SgrAI CRCCGGYG 2 cut(s) 520, 582
SgrBI CCGCGG 2 cut(s) 112, 465
SinI GGWCC 2 cut(s) 318, 1247
SmaI CCCGGG 1 cut(s) 1167
SmiMI CAYNNNNRTG 1 cut(s) 962
SmlI CTYRAG 1 cut(s) 1894
SmoI CTYRAG 1 cut(s) 1894
SpeI ACTAGT 2 cut(s) 1037, 1271
Sse9I AATT 7 cut(s) 262, 550, 610, 616, 637, 1563, 1783
SspDI GGCGCC 1 cut(s) 256
SspMI CTAG 8 cut(s) 555, 717, 1038, 1152, 1215, 1272, 1608, 1619
StyD4I CCNGG 9 cut(s) 206, 304, 314, 392, 593, 1018, 1164, 1165, 1307
StyI CCWWGG 2 cut(s) 450, 1788
TaaI ACNGT 6 cut(s) 187, 659, 908, 1126, 1373, 1889
TaiI ACGT 4 cut(s) 1184, 1828, 1865, 1924
TaqI TCGA 9 cut(s) 167, 239, 515, 614, 629, 758, 1049, 1202, 1748
TasI AATT 7 cut(s) 262, 550, 610, 616, 637, 1563, 1783
TatI WGTACW 1 cut(s) 132
TfiI GAWTC 2 cut(s) 1102, 1703
Tru1I TTAA 1 cut(s) 1500
Tru9I TTAA 1 cut(s) 1500
TscAI CASTG 3 cut(s) 895, 913, 1388
TseFI GTSAC 4 cut(s) 382, 1194, 1492, 1810
TseI GCWGC 8 cut(s) 8, 226, 433, 476, 727, 1061, 1718, 1937
Tsp45I GTSAC 4 cut(s) 382, 1194, 1492, 1810
TspDTI ATGAA 4 cut(s) 257, 1644, 1752, 1796
TspMI CCCGGG 1 cut(s) 1165
TspRI CASTG 3 cut(s) 895, 913, 1388
Van91I CCANNNNNTGG 1 cut(s) 1667
VpaK11BI GGWCC 2 cut(s) 318, 1247
XapI RAATTY 6 cut(s) 262, 550, 610, 616, 637, 1783
XceI RCATGY 2 cut(s) 139, 1354
XmaI CCCGGG 1 cut(s) 1165
XmiI GTMKAC 2 cut(s) 515, 1924
XspI CTAG 8 cut(s) 555, 717, 1038, 1152, 1215, 1272, 1608, 1619
ZraI GACGTC 1 cut(s) 1182
Zsp2I ATGCAT 1 cut(s) 1462
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.