Rh6AG059200

QWRF motif-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0005693
Physical Location & Seq
Forward (+)
8924623 .. 8931459
6837 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG059200.1

Sequence Viewer

Length: 1986 bp
ATGGTGGCTGCCATTTCCGACCCGACCCATCTCCAGAACCAATCTCGGCCGCCGCTTCTTCCCTCAGACAATGCCTCCTCCGCCGCCGCCGCAGTTCAACGACGCGTCGTCCTACGCGGCAGGCAGGTCTCCTCCAAGTACATGTCCACTCCTTCTCCTTCTTCTTCAAACTCGACTTCTACCTCTACTACAGTCTCCTCCTCCGCGTCTTCCAGGAGATGCCCTTCGCCGCTGCTCTCGCGGTCGATGAACTCGTCTCCGGCGCCGAATTCGGGCCCGAAGCGGGCCCAATCGGCCGACCGGAGGCGACCCGGTACGGCCCGGACCATACCGGAGCACCGGCTGAGCAATGCGGGTTCCGAAGTCTCCGCCGCCACCAGACTCCTCGTCACTTCCACCCGGAGCCTCTCGGTTTCGTTTCAGGGCGAGGCGTTCTCGCTGCCGATTAGTAAGACCAAGGCGGCGGCCGCGGCGGCAGGGGCTGCTCCGGCGAGGAAGGCCACGCCGGAGCGGAGGAGGTCGACGCCGGTGAGAAGAGAAGGAGATCAGGTGGAGAATTCTAGGCCCTCCGGCGGTGGTGATCAGCACCGGTGGCCGGCCAGGAGTCGGCAACCGAATTCGAATTCGGGTTATTCGAACCCGAATTTGAGTAGGAGTATGGACTGTAGTAGTGTGGAGAAGAAGAGTGTGAATGTGAATGGGGTTGGATCTGGTGTGGTAGCTAGGGCGTTGCAGCAGTCTATGATTGAGGAGAGTAGAGGATCGAGGTCAAGATCGTCTTTTGATGGTAGGTTGAGCTTGGATTTGGGGCATTTTGGGGGCAATGCTGAGGTTTTGAGGGGTGCTCAGCACAACCCAGATGCTAATAATGAGTCTTCTGTGCCGTCTGATCTCACTGCTTCCGATACAGACAGTGTTTCTTCGGGGAGCACTTCGGGTGTTCAGGACTCCAACAATGTGGCTTCTGCCAAGGCCGCACGAGTGGCCGCCCCTCGCGGCATTGCTGTGTCTGCAAGGTTCTGGCAGGAGACGAATAGCAGGTTGCGGAGGTTGCAGGATCCCGGTTCACCTTTGGCGACTAGTCCTGTGTCGAGAGCAGGGGCGGCGGGGAAGTATATGCAGGCGAAAAAGTTGAATGGGGAGAATCCTGTGTCGTCTCCGAGGACAGTGGCTTCTCCCATTAGAGGAGCTACTAGGCCGGCTTCCCCGGGGAAGCTGTGGACGTCTTCGTCTTTGTCACCTTCGAGGGGGAATGCTAGTCCTTCAAGGGTGAGGAATAGTTTTAGTGGTCCATTGAGTAGTGGTTATGCTGCTAGTACACCTTCGTTTCTTAGTTTCTCTATTGATACCCGGAGAGGGAAGAAGGGGGAAGACCGCATTGTTGATGCACACATGCTCAGGCTTCTGTATAACCGTTATCTGCAGTGGCGGTTTGTAAATGCAAGAGCAGATGCTACATACATGGTGCAGAGAGTGAATGCAGAGGAAAATCTTTGGAATGCATGGGTAACTATCTCAGAGCTACGACATAATGTCACACTTAAAAGGATCAAGTTACTTTTGTTGAGGCATAAGTTGAAGCTAACTTCTATCCTCAAAGGACAAATTACTTATTTGGAAGAATGGGCTCTTCTGGACAGAGATCACTCTAGTTCTTTGCTAGGAGCGAATGAAGCTTTGCAAGCCAGTACCCTTCGTCTTCCAGTCGTTGGAAAAGCAATAGTTGACTTTCAAAAGCTGAAAGATTCTGTGGGCTCAGCAGCTGATGTGATGCAGGCAATGGCATCCTCGATATGCTTGCTTTCATCAAAGGTGGAAGAAATGAATTCCTTGGTATCTGAACTTGTGAAGGTGACTACAACAGAACGTCTTGTGCTTGAACAATGCAAAGATTTTTTGTCAACGTTATCAGCCATGCAAGTCAAGGACTGTAGCTTGAGAACACATATAATACAACTAAAACGTCTACCATTACCCGCAGCCTGA

Protein Analysis

661

Amino Acids

70.86

Weight (kDa)

10.75

Isoelectric Point (pI)

62.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
QWRF PF04484 305 - 627 1.4e-112 QWRF family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1228
AatII GACGTC 1 cut(s) 1226
Acc36I ACCTGC 2 cut(s) 115, 1029
AccB1I GGYRCC 1 cut(s) 262
AccB7I CCANNNNNTGG 1 cut(s) 1709
AccBSI CCGCTC 1 cut(s) 511
AccI GTMKAC 2 cut(s) 521, 1966
AccII CGCG 6 cut(s) 105, 117, 206, 241, 470, 996
AclI AACGTT 1 cut(s) 1904
AclWI GGATC 5 cut(s) 715, 769, 1052, 1065, 1556
AcoI YGGCCR 6 cut(s) 47, 294, 465, 593, 597, 984
AcsI RAATTY 6 cut(s) 268, 556, 616, 622, 643, 1825
AcyI GRCGYC 3 cut(s) 263, 524, 1223
AfaI GTAC 4 cut(s) 140, 316, 1318, 1690
AflIII ACRYGT 2 cut(s) 103, 141
AgeI ACCGGT 1 cut(s) 588
AgsI TTSAA 7 cut(s) 98, 168, 1135, 1266, 1579, 1733, 1880
AhdI GACNNNNNGTC 3 cut(s) 107, 386, 1532
AhlI ACTAGT 1 cut(s) 1079
AjnI CCWGG 2 cut(s) 212, 599
Alw21I GWGCWC 3 cut(s) 339, 847, 932
Alw26I GTCTC 6 cut(s) 133, 199, 261, 370, 1022, 1161
AlwI GGATC 5 cut(s) 715, 769, 1052, 1065, 1556
AlwNI CAGNNNCTG 2 cut(s) 482, 1763
Ama87I CYCGRG 1 cut(s) 1207
ApaI GGGCCC 2 cut(s) 278, 289
ApeKI GCWGC 8 cut(s) 8, 232, 439, 482, 733, 1310, 1760, 1979
ApoI RAATTY 6 cut(s) 268, 556, 616, 622, 643, 1825
AsiGI ACCGGT 1 cut(s) 588
AspLEI GCGC 1 cut(s) 265
AspS9I GGNCC 8 cut(s) 274, 275, 285, 286, 319, 324, 564, 1289
AsuC2I CCSGG 7 cut(s) 312, 322, 400, 1062, 1208, 1209, 1351
AsuHPI GGTGA 6 cut(s) 541, 590, 1059, 1230, 1282, 1864
AsuII TTCGAA 2 cut(s) 620, 635
AvaI CYCGRG 1 cut(s) 1207
AvaII GGWCC 2 cut(s) 324, 1289
BaeGI GKGCMC 2 cut(s) 278, 289
BamHI GGATCC 1 cut(s) 1057
BanI GGYRCC 1 cut(s) 262
BanII GRGCYC 4 cut(s) 278, 289, 1630, 1757
BauI CACGAG 1 cut(s) 978
BbsI GAAGAC 5 cut(s) 201, 867, 1218, 1377, 1691
Bbv12I GWGCWC 3 cut(s) 339, 847, 932
BbvCI CCTCAGC 1 cut(s) 828
BbvI GCAGC 6 cut(s) 219, 426, 469, 745, 1297, 1772
BccI CCATC 2 cut(s) 36, 779
BceAI ACGGC 2 cut(s) 333, 868
BcgI CGANNNNNNTGC 2 cut(s) 1780, 1814
BciT130I CCWGG 2 cut(s) 214, 601
BclI TGATCA 1 cut(s) 580
BcnI CCSGG 7 cut(s) 312, 322, 400, 1062, 1208, 1209, 1351
BcoDI GTCTC 6 cut(s) 133, 199, 261, 370, 1022, 1161
BcuI ACTAGT 1 cut(s) 1079
BfaI CTAG 8 cut(s) 561, 723, 1080, 1194, 1257, 1314, 1650, 1661
BfmI CTRYAG 4 cut(s) 189, 664, 1421, 1930
BfoI RGCGCY 1 cut(s) 266
BfuAI ACCTGC 2 cut(s) 115, 1029
BglI GCCNNNNNGGC 2 cut(s) 293, 473
BlpI GCTNAGC 3 cut(s) 344, 846, 1756
Bme1390I CCNGG 9 cut(s) 214, 312, 322, 400, 601, 1062, 1208, 1209, 1351
Bme18I GGWCC 2 cut(s) 324, 1289
BmeRI GACNNNNNGTC 3 cut(s) 107, 386, 1532
BmeT110I CYCGRG 1 cut(s) 1207
BmgT120I GGNCC 8 cut(s) 274, 275, 285, 286, 319, 324, 564, 1289
BmiI GGNNCC 6 cut(s) 264, 276, 287, 358, 404, 1059
BmrFI CCNGG 9 cut(s) 214, 312, 322, 400, 601, 1062, 1208, 1209, 1351
BmsI GCATC 6 cut(s) 209, 850, 1375, 1441, 1761, 1793
BpiI GAAGAC 5 cut(s) 201, 867, 1218, 1377, 1691
BplI GAGNNNNNCTC 4 cut(s) 419, 451, 829, 861
BpmI CTGGAG 1 cut(s) 17
Bpu10I CCTNAGC 2 cut(s) 828, 1397
Bpu1102I GCTNAGC 3 cut(s) 344, 846, 1756
Bpu14I TTCGAA 2 cut(s) 620, 635
BpuEI CTTGAG 1 cut(s) 1957
BpuMI CCSGG 7 cut(s) 312, 322, 400, 1062, 1208, 1209, 1351
BsaHI GRCGYC 3 cut(s) 263, 524, 1223
BsaI GGTCTC 1 cut(s) 133
BsaJI CCNNGG 8 cut(s) 456, 468, 969, 1160, 1206, 1207, 1208, 1830
BsaWI WCCGGW 3 cut(s) 300, 331, 588
BsaXI ACNNNNNCTCC 6 cut(s) 59, 89, 139, 169, 534, 564
Bse118I RCCGGY 5 cut(s) 339, 526, 588, 595, 1198
Bse1I ACTGG 2 cut(s) 1686, 1703
Bse3DI GCAATG 4 cut(s) 355, 829, 999, 1785
BseBI CCWGG 2 cut(s) 214, 601
BseDI CCNNGG 8 cut(s) 456, 468, 969, 1160, 1206, 1207, 1208, 1830
BseGI GGATG 1 cut(s) 1784
BseMI GCAATG 4 cut(s) 355, 829, 999, 1785
BseMII CTCAG 7 cut(s) 78, 335, 819, 860, 1411, 1530, 1770
BseNI ACTGG 2 cut(s) 1686, 1703
BseRI GAGGAG 8 cut(s) 67, 121, 187, 190, 374, 529, 764, 1200
BseSI GKGCMC 2 cut(s) 278, 289
BseX3I CGGCCG 3 cut(s) 47, 294, 465
BseXI GCAGC 6 cut(s) 219, 426, 469, 745, 1297, 1772
BsgI GTGCAG 1 cut(s) 1487
Bsh1236I CGCG 6 cut(s) 105, 117, 206, 241, 470, 996
Bsh1285I CGRYCG 5 cut(s) 50, 245, 297, 301, 468
BshNI GGYRCC 1 cut(s) 262
BshTI ACCGGT 1 cut(s) 588
BsiEI CGRYCG 5 cut(s) 50, 245, 297, 301, 468
BsiHKAI GWGCWC 3 cut(s) 339, 847, 932
BsiHKCI CYCGRG 1 cut(s) 1207
BsmAI GTCTC 6 cut(s) 133, 199, 261, 370, 1022, 1161
BsmBI CGTCTC 3 cut(s) 261, 1022, 1161
BsmI GAATGC 3 cut(s) 1258, 1483, 1504
Bso31I GGTCTC 1 cut(s) 133
BsoBI CYCGRG 1 cut(s) 1207
Bsp119I TTCGAA 2 cut(s) 620, 635
Bsp120I GGGCCC 2 cut(s) 274, 285
Bsp1286I GDGCHC 7 cut(s) 278, 289, 339, 847, 932, 1630, 1757
Bsp143I GATC 9 cut(s) 544, 580, 707, 761, 773, 889, 1057, 1548, 1642
Bsp1720I GCTNAGC 3 cut(s) 344, 846, 1756
BspCNI CTCAG 7 cut(s) 77, 336, 820, 859, 1410, 1529, 1769
BspFNI CGCG 6 cut(s) 105, 117, 206, 241, 470, 996
BspLI GGNNCC 6 cut(s) 264, 276, 287, 358, 404, 1059
BspMAI CTGCAG 1 cut(s) 1425
BspMI ACCTGC 2 cut(s) 115, 1029
BspPI GGATC 5 cut(s) 715, 769, 1052, 1065, 1556
BspQI GCTCTTC 1 cut(s) 1635
BspT104I TTCGAA 2 cut(s) 620, 635
BspT107I GGYRCC 1 cut(s) 262
BspTNI GGTCTC 1 cut(s) 133
BsrBI CCGCTC 1 cut(s) 511
BsrDI GCAATG 4 cut(s) 355, 829, 999, 1785
BsrFI RCCGGY 5 cut(s) 339, 526, 588, 595, 1198
BsrI ACTGG 2 cut(s) 1686, 1703
BssAI RCCGGY 5 cut(s) 339, 526, 588, 595, 1198
BssECI CCNNGG 8 cut(s) 456, 468, 969, 1160, 1206, 1207, 1208, 1830
BssMI GATC 9 cut(s) 544, 580, 707, 761, 773, 889, 1057, 1548, 1642
BssNI GRCGYC 3 cut(s) 263, 524, 1223
BssSI CACGAG 1 cut(s) 978
BssT1I CCWWGG 3 cut(s) 456, 969, 1830
Bst2BI CACGAG 1 cut(s) 978
Bst2UI CCWGG 2 cut(s) 214, 601
Bst4CI ACNGT 6 cut(s) 193, 665, 914, 1168, 1415, 1931
Bst6I CTCTTC 3 cut(s) 529, 677, 1635
BstACI GRCGYC 3 cut(s) 263, 524, 1223
BstAPI GCANNNNNTGC 1 cut(s) 482
BstBI TTCGAA 2 cut(s) 620, 635
BstC8I GCNNGC 8 cut(s) 122, 285, 597, 1122, 1200, 1683, 1776, 1799
BstDEI CTNAG 8 cut(s) 64, 344, 828, 846, 1331, 1397, 1516, 1756
BstDSI CCRYGG 1 cut(s) 468
BstF5I GGATG 1 cut(s) 1784
BstFNI CGCG 6 cut(s) 105, 117, 206, 241, 470, 996
BstH2I RGCGCY 1 cut(s) 266
BstHHI GCGC 1 cut(s) 265
BstKTI GATC 9 cut(s) 547, 583, 710, 764, 776, 892, 1060, 1551, 1645
BstMAI GTCTC 6 cut(s) 133, 199, 261, 370, 1022, 1161
BstMBI GATC 9 cut(s) 544, 580, 707, 761, 773, 889, 1057, 1548, 1642
BstMCI CGRYCG 5 cut(s) 50, 245, 297, 301, 468
BstNI CCWGG 2 cut(s) 214, 601
BstNSI RCATGY 2 cut(s) 145, 1396
BstSCI CCNGG 9 cut(s) 212, 310, 320, 398, 599, 1060, 1206, 1207, 1349
BstSFI CTRYAG 4 cut(s) 189, 664, 1421, 1930
BstSLI GKGCMC 2 cut(s) 278, 289
BstUI CGCG 6 cut(s) 105, 117, 206, 241, 470, 996
BstV1I GCAGC 6 cut(s) 219, 426, 469, 745, 1297, 1772
BstV2I GAAGAC 5 cut(s) 201, 867, 1218, 1377, 1691
BstX2I RGATCY 2 cut(s) 707, 1057
BstXI CCANNNNNNTGG 1 cut(s) 958
BstYI RGATCY 2 cut(s) 707, 1057
BstZI CGGCCG 3 cut(s) 47, 294, 465
BtgI CCRYGG 1 cut(s) 468
BtsCI GGATG 1 cut(s) 1784
BtsI GCAGTG 2 cut(s) 894, 1430
BtsIMutI CAGTG 4 cut(s) 894, 919, 1173, 1430
BveI ACCTGC 2 cut(s) 115, 1029
Cac8I GCNNGC 8 cut(s) 122, 285, 597, 1122, 1200, 1683, 1776, 1799
CaiI CAGNNNCTG 2 cut(s) 482, 1763
CciNI GCGGCCGC 1 cut(s) 465
CfoI GCGC 1 cut(s) 265
Cfr10I RCCGGY 5 cut(s) 339, 526, 588, 595, 1198
Cfr13I GGNCC 8 cut(s) 274, 275, 285, 286, 319, 324, 564, 1289
Cfr42I CCGCGG 1 cut(s) 471
Cfr9I CCCGGG 1 cut(s) 1207
CseI GACGC 4 cut(s) 94, 111, 195, 532
Csp6I GTAC 4 cut(s) 139, 315, 1317, 1689
CspAI ACCGGT 1 cut(s) 588
CviAII CATG 5 cut(s) 142, 1393, 1462, 1503, 1915
CviQI GTAC 4 cut(s) 139, 315, 1317, 1689
DdeI CTNAG 8 cut(s) 64, 344, 828, 846, 1331, 1397, 1516, 1756
DinI GGCGCC 1 cut(s) 264
DpnI GATC 9 cut(s) 546, 582, 709, 763, 775, 891, 1059, 1550, 1644
DpnII GATC 9 cut(s) 544, 580, 707, 761, 773, 889, 1057, 1548, 1642
DrdI GACNNNNNNGTC 1 cut(s) 1228
DriI GACNNNNNGTC 3 cut(s) 107, 386, 1532
DseDI GACNNNNNNGTC 1 cut(s) 1228
EaeI YGGCCR 6 cut(s) 47, 294, 465, 593, 597, 984
EagI CGGCCG 3 cut(s) 47, 294, 465
Eam1104I CTCTTC 3 cut(s) 529, 677, 1635
Eam1105I GACNNNNNGTC 3 cut(s) 107, 386, 1532
EarI CTCTTC 3 cut(s) 529, 677, 1635
EciI GGCGGA 2 cut(s) 70, 358
EclXI CGGCCG 3 cut(s) 47, 294, 465
Eco130I CCWWGG 3 cut(s) 456, 969, 1830
Eco24I GRGCYC 4 cut(s) 278, 289, 1630, 1757
Eco31I GGTCTC 1 cut(s) 133
Eco47I GGWCC 2 cut(s) 324, 1289
Eco52I CGGCCG 3 cut(s) 47, 294, 465
Eco88I CYCGRG 1 cut(s) 1207
EcoO109I RGGNCCY 1 cut(s) 564
EcoRI GAATTC 5 cut(s) 268, 556, 616, 622, 1825
EcoRII CCWGG 2 cut(s) 212, 599
EcoT14I CCWWGG 3 cut(s) 456, 969, 1830
EcoT22I ATGCAT 1 cut(s) 1504
EcoT38I GRGCYC 4 cut(s) 278, 289, 1630, 1757
EgeI GGCGCC 1 cut(s) 264
EheI GGCGCC 1 cut(s) 264
ErhI CCWWGG 3 cut(s) 456, 969, 1830
Esp3I CGTCTC 3 cut(s) 261, 1022, 1161
FaeI CATG 5 cut(s) 145, 1396, 1465, 1506, 1918
FalI AAGNNNNNCTT 2 cut(s) 763, 795
FatI CATG 5 cut(s) 141, 1392, 1461, 1502, 1914
FauI CCCGC 4 cut(s) 276, 346, 1099, 1984
FbaI TGATCA 1 cut(s) 580
FblI GTMKAC 2 cut(s) 521, 1966
FokI GGATG 1 cut(s) 1771
FriOI GRGCYC 4 cut(s) 278, 289, 1630, 1757
FseI GGCCGGCC 1 cut(s) 599
FspBI CTAG 8 cut(s) 561, 723, 1080, 1194, 1257, 1314, 1650, 1661
GlaI GCGC 1 cut(s) 264
GsuI CTGGAG 1 cut(s) 17
HaeII RGCGCY 1 cut(s) 266
HgaI GACGC 4 cut(s) 94, 111, 195, 532
HhaI GCGC 1 cut(s) 265
Hin1I GRCGYC 3 cut(s) 263, 524, 1223
Hin1II CATG 5 cut(s) 145, 1396, 1465, 1506, 1918
Hin6I GCGC 1 cut(s) 263
HinP1I GCGC 1 cut(s) 263
HincII GTYRAC 3 cut(s) 522, 1726, 1902
HindII GTYRAC 3 cut(s) 522, 1726, 1902
HindIII AAGCTT 1 cut(s) 1674
HinfI GANTC 6 cut(s) 381, 604, 872, 947, 1144, 1745
HphI GGTGA 6 cut(s) 541, 590, 1059, 1230, 1282, 1864
Hpy166II GTNNAC 8 cut(s) 147, 522, 1067, 1221, 1319, 1726, 1902, 1967
Hpy188I TCNGA 8 cut(s) 19, 67, 361, 889, 904, 1161, 1519, 1840
Hpy188III TCNNGA 5 cut(s) 34, 771, 944, 1092, 1634
Hpy8I GTNNAC 8 cut(s) 147, 522, 1067, 1221, 1319, 1726, 1902, 1967
Hpy99I CGWCG 3 cut(s) 105, 110, 526
HpyCH4III ACNGT 6 cut(s) 193, 665, 914, 1168, 1415, 1931
HpyCH4IV ACGT 4 cut(s) 1223, 1867, 1904, 1963
HpyF3I CTNAG 8 cut(s) 64, 344, 828, 846, 1331, 1397, 1516, 1756
HpySE526I ACGT 4 cut(s) 1223, 1867, 1904, 1963
Hsp92I GRCGYC 3 cut(s) 263, 524, 1223
Hsp92II CATG 5 cut(s) 145, 1396, 1465, 1506, 1918
HspAI GCGC 1 cut(s) 263
KasI GGCGCC 1 cut(s) 262
KroI GCCGGC 2 cut(s) 595, 1198
KroNI GCCGGC 2 cut(s) 597, 1200
Ksp22I TGATCA 1 cut(s) 580
KspI CCGCGG 1 cut(s) 471
Kzo9I GATC 9 cut(s) 544, 580, 707, 761, 773, 889, 1057, 1548, 1642
LguI GCTCTTC 1 cut(s) 1635
LmnI GCTCC 7 cut(s) 334, 402, 490, 508, 927, 1187, 1664
Lsp1109I GCAGC 6 cut(s) 219, 426, 469, 745, 1297, 1772
LweI GCATC 6 cut(s) 209, 850, 1375, 1441, 1761, 1793
MaeI CTAG 8 cut(s) 561, 723, 1080, 1194, 1257, 1314, 1650, 1661
MaeII ACGT 4 cut(s) 1223, 1867, 1904, 1963
MaeIII GTNAC 6 cut(s) 388, 1236, 1507, 1534, 1554, 1852
MalI GATC 9 cut(s) 546, 582, 709, 763, 775, 891, 1059, 1550, 1644
MbiI CCGCTC 1 cut(s) 511
MboI GATC 9 cut(s) 544, 580, 707, 761, 773, 889, 1057, 1548, 1642
MflI RGATCY 2 cut(s) 707, 1057
MhlI GDGCHC 7 cut(s) 278, 289, 339, 847, 932, 1630, 1757
MluCI AATT 7 cut(s) 268, 556, 616, 622, 643, 1605, 1825
MluI ACGCGT 1 cut(s) 103
Mly113I GGCGCC 1 cut(s) 263
MlyI GAGTC 4 cut(s) 375, 613, 881, 941
MmeI TCCRAC 4 cut(s) 42, 685, 975, 1690
Mph1103I ATGCAT 1 cut(s) 1504
MroNI GCCGGC 2 cut(s) 595, 1198
MseI TTAA 1 cut(s) 1542
MslI CAYNNNNRTG 1 cut(s) 1004
MspA1I CMGCKG 3 cut(s) 232, 470, 1763
MspR9I CCNGG 9 cut(s) 214, 312, 322, 400, 601, 1062, 1208, 1209, 1351
Mva1269I GAATGC 3 cut(s) 1258, 1483, 1504
MvaI CCWGG 2 cut(s) 214, 601
MvnI CGCG 6 cut(s) 105, 117, 206, 241, 470, 996
NaeI GCCGGC 2 cut(s) 597, 1200
NarI GGCGCC 1 cut(s) 263
NciI CCSGG 7 cut(s) 312, 322, 400, 1062, 1208, 1209, 1351
NdeII GATC 9 cut(s) 544, 580, 707, 761, 773, 889, 1057, 1548, 1642
NgoMIV GCCGGC 2 cut(s) 595, 1198
NlaIII CATG 5 cut(s) 145, 1396, 1465, 1506, 1918
NlaIV GGNNCC 6 cut(s) 264, 276, 287, 358, 404, 1059
NmeAIII GCCGAG 1 cut(s) 25
NmuCI GTSAC 4 cut(s) 388, 1236, 1534, 1852
NotI GCGGCCGC 1 cut(s) 465
NsiI ATGCAT 1 cut(s) 1504
NspI RCATGY 2 cut(s) 145, 1396
NspV TTCGAA 2 cut(s) 620, 635
PciI ACATGT 1 cut(s) 141
PciSI GCTCTTC 1 cut(s) 1635
PcsI WCGNNNNNNNCGW 2 cut(s) 251, 632
PctI GAATGC 3 cut(s) 1258, 1483, 1504
PdiI GCCGGC 2 cut(s) 597, 1200
PfeI GAWTC 2 cut(s) 1144, 1745
PflMI CCANNNNNTGG 1 cut(s) 1709
PfoI TCCNGGA 1 cut(s) 212
PinAI ACCGGT 1 cut(s) 588
PleI GAGTC 4 cut(s) 375, 612, 880, 941
PluTI GGCGCC 1 cut(s) 266
PpsI GAGTC 4 cut(s) 375, 612, 880, 941
PscI ACATGT 1 cut(s) 141
Psp1406I AACGTT 1 cut(s) 1904
Psp6I CCWGG 2 cut(s) 212, 599
PspGI CCWGG 2 cut(s) 212, 599
PspN4I GGNNCC 6 cut(s) 264, 276, 287, 358, 404, 1059
PspOMI GGGCCC 2 cut(s) 274, 285
PspPI GGNCC 8 cut(s) 274, 275, 285, 286, 319, 324, 564, 1289
PstI CTGCAG 1 cut(s) 1425
PstNI CAGNNNCTG 2 cut(s) 482, 1763
PsuI RGATCY 2 cut(s) 707, 1057
PvuII CAGCTG 1 cut(s) 1763
RigI GGCCGGCC 1 cut(s) 599
RsaI GTAC 4 cut(s) 140, 316, 1318, 1690
RsaNI GTAC 4 cut(s) 139, 315, 1317, 1689
RseI CAYNNNNRTG 1 cut(s) 1004
SacII CCGCGG 1 cut(s) 471
SalI GTCGAC 1 cut(s) 520
SapI GCTCTTC 1 cut(s) 1635
SaqAI TTAA 1 cut(s) 1542
Sau3AI GATC 9 cut(s) 544, 580, 707, 761, 773, 889, 1057, 1548, 1642
Sau96I GGNCC 8 cut(s) 274, 275, 285, 286, 319, 324, 564, 1289
SchI GAGTC 4 cut(s) 375, 613, 881, 941
ScrFI CCNGG 9 cut(s) 214, 312, 322, 400, 601, 1062, 1208, 1209, 1351
SduI GDGCHC 7 cut(s) 278, 289, 339, 847, 932, 1630, 1757
SfaNI GCATC 6 cut(s) 209, 850, 1375, 1441, 1761, 1793
SfcI CTRYAG 4 cut(s) 189, 664, 1421, 1930
SfiI GGCCNNNNNGGCC 1 cut(s) 293
SfoI GGCGCC 1 cut(s) 264
Sfr303I CCGCGG 1 cut(s) 471
SfuI TTCGAA 2 cut(s) 620, 635
SgrAI CRCCGGYG 2 cut(s) 526, 588
SgrBI CCGCGG 1 cut(s) 471
SinI GGWCC 2 cut(s) 324, 1289
SmaI CCCGGG 1 cut(s) 1209
SmiMI CAYNNNNRTG 1 cut(s) 1004
SmlI CTYRAG 1 cut(s) 1936
SmoI CTYRAG 1 cut(s) 1936
SpeI ACTAGT 1 cut(s) 1079
Sse9I AATT 7 cut(s) 268, 556, 616, 622, 643, 1605, 1825
SspDI GGCGCC 1 cut(s) 262
SspMI CTAG 8 cut(s) 561, 723, 1080, 1194, 1257, 1314, 1650, 1661
StyD4I CCNGG 9 cut(s) 212, 310, 320, 398, 599, 1060, 1206, 1207, 1349
StyI CCWWGG 3 cut(s) 456, 969, 1830
TaaI ACNGT 6 cut(s) 193, 665, 914, 1168, 1415, 1931
TaiI ACGT 4 cut(s) 1226, 1870, 1907, 1966
TaqI TCGA 9 cut(s) 173, 245, 521, 620, 635, 764, 1091, 1244, 1790
TasI AATT 7 cut(s) 268, 556, 616, 622, 643, 1605, 1825
TatI WGTACW 2 cut(s) 138, 1316
TfiI GAWTC 2 cut(s) 1144, 1745
Tru1I TTAA 1 cut(s) 1542
Tru9I TTAA 1 cut(s) 1542
TscAI CASTG 4 cut(s) 901, 919, 1173, 1430
TseFI GTSAC 4 cut(s) 388, 1236, 1534, 1852
TseI GCWGC 8 cut(s) 8, 232, 439, 482, 733, 1310, 1760, 1979
Tsp45I GTSAC 4 cut(s) 388, 1236, 1534, 1852
TspDTI ATGAA 4 cut(s) 263, 1686, 1794, 1838
TspMI CCCGGG 1 cut(s) 1207
TspRI CASTG 4 cut(s) 901, 919, 1173, 1430
Van91I CCANNNNNTGG 1 cut(s) 1709
VpaK11BI GGWCC 2 cut(s) 324, 1289
XapI RAATTY 6 cut(s) 268, 556, 616, 622, 643, 1825
XceI RCATGY 2 cut(s) 145, 1396
XmaI CCCGGG 1 cut(s) 1207
XmiI GTMKAC 2 cut(s) 521, 1966
XspI CTAG 8 cut(s) 561, 723, 1080, 1194, 1257, 1314, 1650, 1661
ZraI GACGTC 1 cut(s) 1224
Zsp2I ATGCAT 1 cut(s) 1504
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.