FvH4_2g04100

N-acetyltransferase HLS1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0004042
Physical Location & Seq
Forward (+)
3234262 .. 3235870
1609 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g04100.t1

Sequence Viewer

Length: 1218 bp
ATGGAAGAGGAAATGAGAGCCAATATGTTAGTGATTAGAGAGTTGGACAAGAACGAGGACACTCAAAAGGTTGAAGAGCTAGAGAGAAGGTGTGAGGCTGGTTCGTGTGGTGAAATGTCACTCTTTACTGATCTTTTGGGTGACCCGATTTGTCGAATCCGAAACTCGCCTTCCTTCCTCATGCTGGTGGCTGAAATTGGTCCTGGAAAAGAAATCGTTGGGATCATCAGGGGTTGCATCAAAGTAGTTTCTTGTGGTCAACACCTCAGAAGGCCTAGCAATGAAGATTCTGATCTTAAAATCGAGCCCATTTGCACCAAACTTGCTTACATTCTTGGCCTTAGAGTGTCTCCTTCACACAGGAGAAAAGGTATTGCTCTGAAACTTGTTAACAGAATGGAGGAATGGTTTCGAAGAAATGGTGCAGAGTACTCATACATGGCTACTGAGCTGGACAATAAACCTTCACTAAACCTGTTCACCAAAAAATGCAACTACACCAAATTTCGTACCCCTTCAATATTGGTCCAACCGGTCTTTGCTCATCGAGTCAAAACTCACCGCAGACAAATCACCATTATTGAACTCACCCCACCAGAGGCTGAGACCCTCTACCGGCGGCAATTGTCCGCCACCGAGTTTTTTCCTCAGGACATTGATAGAATTGTGCAAAACAAGCACAATGCTGGTACGTTTTTAGCTGTCCCAACCGAGTATTTGTGGACGGGTTTGGACAGGTTTCTATCTGACCCGCCGGAGTCATGGGCTGTTATGAGTGCATGGAACTGTAAGGAGGTGTTCAAGTTGGAGATCAAAGGCGCGTCGCTTGCACGGCGCTTGGCTGCCAAGGCAACACGCATGGTGGACAAGGCATTGCCTTGGCTGAGGATACCCTCGGTGCCTGATGTTTTTACGCCATTTGGGTTGCTTTTCCTGTATGGATTAGGAGGACAAGGCCAACAGGCAGATAAGATGGCAAAGGTGCTTTGTGGCCATGCGCATAACATAGCTAAGGAATTGGGGTGTGGGGTGGTGGTGACTGAAGTGGCTAGCCAAGAACCGTTGAGGTCAGCAATACCGCATTGGAAGCGGCTATCCTGCGCAGAGGATGTGTGGTGCATGAAGAGGCTTGTTGAAGACTATAGTGATGGGTCACTAGGGGACTGGTCGAAATCTCCACCTGGGTTGTCAATTTTTGTTGATCCAAGGGAATTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Acetyltransf_1

Protein Analysis

406

Amino Acids

45.9

Weight (kDa)

8.52

Isoelectric Point (pI)

55.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Acetyltransf_1 PF00583 55 - 166 1.3e-13 Acetyltransferase (GNAT) family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 999, 1102
AccB1I GGYRCC 1 cut(s) 898
AccII CGCG 1 cut(s) 821
AciI CCGC 6 cut(s) 562, 619, 630, 752, 1079, 1090
AclWI GGATC 2 cut(s) 230, 1196
AcoI YGGCCR 1 cut(s) 991
AcsI RAATTY 2 cut(s) 503, 1211
AcuI CTGAAG 1 cut(s) 1062
AfaI GTAC 3 cut(s) 431, 511, 691
AfiI CCNNNNNNNGG 3 cut(s) 184, 598, 615
AgeI ACCGGT 1 cut(s) 532
AgsI TTSAA 5 cut(s) 74, 519, 584, 802, 1136
AjnI CCWGG 2 cut(s) 202, 1180
AluBI AGCT 4 cut(s) 79, 451, 701, 1010
AluI AGCT 4 cut(s) 79, 451, 701, 1010
Alw26I GTCTC 2 cut(s) 354, 599
AlwI GGATC 2 cut(s) 230, 1196
AlwNI CAGNNNCTG 1 cut(s) 602
AoxI GGCC 4 cut(s) 272, 337, 955, 991
ApeKI GCWGC 1 cut(s) 842
ApoI RAATTY 2 cut(s) 503, 1211
AsiGI ACCGGT 1 cut(s) 532
Asp700I GAANNNNTTC 1 cut(s) 408
AspLEI GCGC 4 cut(s) 821, 837, 1000, 1103
AspS9I GGNCC 2 cut(s) 200, 526
AsuHPI GGTGA 7 cut(s) 122, 152, 472, 551, 565, 580, 1048
AsuII TTCGAA 1 cut(s) 412
AsuNHI GCTAGC 1 cut(s) 1049
AvaII GGWCC 2 cut(s) 200, 526
AxyI CCTNAGG 1 cut(s) 648
BalI TGGCCA 1 cut(s) 993
BanI GGYRCC 1 cut(s) 898
BanII GRGCYC 1 cut(s) 309
BbsI GAAGAC 1 cut(s) 1143
BbvCI CCTCAGC 1 cut(s) 884
BbvI GCAGC 1 cut(s) 829
BccI CCATC 2 cut(s) 967, 1142
BceAI ACGGC 1 cut(s) 848
BciT130I CCWGG 2 cut(s) 204, 1182
BciVI GTATCC 1 cut(s) 882
BcoDI GTCTC 2 cut(s) 354, 599
BfaI CTAG 5 cut(s) 80, 276, 1050, 1157, 1216
BfmI CTRYAG 1 cut(s) 1141
BfoI RGCGCY 1 cut(s) 838
BfuI GTATCC 1 cut(s) 882
BisI GCNGC 3 cut(s) 620, 843, 1091
BlsI GCNGC 3 cut(s) 621, 844, 1092
BmcAI AGTACT 1 cut(s) 431
Bme1390I CCNGG 2 cut(s) 204, 1182
Bme18I GGWCC 2 cut(s) 200, 526
BmgT120I GGNCC 2 cut(s) 200, 526
BmiI GGNNCC 1 cut(s) 900
BmrFI CCNGG 2 cut(s) 204, 1182
BmsI GCATC 1 cut(s) 246
BmtI GCTAGC 1 cut(s) 1053
BpiI GAAGAC 1 cut(s) 1143
Bpu10I CCTNAGC 2 cut(s) 884, 1011
Bpu14I TTCGAA 1 cut(s) 412
BsaBI GATNNNNATC 1 cut(s) 291
BsaI GGTCTC 1 cut(s) 599
BsaJI CCNNGG 5 cut(s) 846, 878, 894, 1181, 1205
BsaWI WCCGGW 1 cut(s) 532
BsaXI ACNNNNNCTCC 4 cut(s) 355, 385, 392, 422
Bsc4I CCNNNNNNNGG 3 cut(s) 184, 598, 615
Bse118I RCCGGY 2 cut(s) 532, 615
Bse1I ACTGG 1 cut(s) 1169
Bse21I CCTNAGG 1 cut(s) 648
Bse3DI GCAATG 2 cut(s) 286, 872
Bse8I GATNNNNATC 1 cut(s) 291
BseBI CCWGG 2 cut(s) 204, 1182
BseDI CCNNGG 5 cut(s) 846, 878, 894, 1181, 1205
BseGI GGATG 1 cut(s) 1114
BseJI GATNNNNATC 1 cut(s) 291
BseLI CCNNNNNNNGG 3 cut(s) 184, 598, 615
BseMI GCAATG 2 cut(s) 286, 872
BseMII CTCAG 5 cut(s) 280, 438, 594, 662, 875
BseNI ACTGG 1 cut(s) 1169
BseXI GCAGC 1 cut(s) 829
BsgI GTGCAG 1 cut(s) 444
Bsh1236I CGCG 1 cut(s) 821
BshFI GGCC 4 cut(s) 274, 339, 957, 993
BshNI GGYRCC 1 cut(s) 898
BshTI ACCGGT 1 cut(s) 532
BsiSI CCGG 3 cut(s) 533, 616, 755
BslFI GGGAC 2 cut(s) 689, 1175
BslI CCNNNNNNNGG 3 cut(s) 184, 598, 615
BsmAI GTCTC 2 cut(s) 354, 599
BsmFI GGGAC 2 cut(s) 689, 1175
BsnI GGCC 4 cut(s) 274, 339, 957, 993
Bso31I GGTCTC 1 cut(s) 599
Bsp119I TTCGAA 1 cut(s) 412
Bsp1286I GDGCHC 1 cut(s) 309
Bsp143I GATC 5 cut(s) 130, 222, 292, 810, 1201
BspACI CCGC 6 cut(s) 562, 619, 630, 752, 1079, 1090
BspANI GGCC 4 cut(s) 274, 339, 957, 993
BspCNI CTCAG 5 cut(s) 279, 439, 595, 661, 876
BspFNI CGCG 1 cut(s) 821
BspLI GGNNCC 1 cut(s) 900
BspOI GCTAGC 1 cut(s) 1053
BspPI GGATC 2 cut(s) 230, 1196
BspQI GCTCTTC 1 cut(s) 69
BspT104I TTCGAA 1 cut(s) 412
BspT107I GGYRCC 1 cut(s) 898
BspTNI GGTCTC 1 cut(s) 599
BsrDI GCAATG 2 cut(s) 286, 872
BsrFI RCCGGY 2 cut(s) 532, 615
BsrI ACTGG 1 cut(s) 1169
BssAI RCCGGY 2 cut(s) 532, 615
BssECI CCNNGG 5 cut(s) 846, 878, 894, 1181, 1205
BssMI GATC 5 cut(s) 130, 222, 292, 810, 1201
BssT1I CCWWGG 3 cut(s) 846, 878, 1205
Bst2UI CCWGG 2 cut(s) 204, 1182
Bst4CI ACNGT 2 cut(s) 788, 1062
Bst6I CTCTTC 2 cut(s) 69, 1118
BstBI TTCGAA 1 cut(s) 412
BstC8I GCNNGC 2 cut(s) 828, 1051
BstDEI CTNAG 7 cut(s) 266, 341, 447, 603, 648, 884, 1011
BstEII GGTNACC 1 cut(s) 140
BstF5I GGATG 1 cut(s) 1114
BstFNI CGCG 1 cut(s) 821
BstH2I RGCGCY 1 cut(s) 838
BstHHI GCGC 4 cut(s) 821, 837, 1000, 1103
BstKTI GATC 5 cut(s) 133, 225, 295, 813, 1204
BstMAI GTCTC 2 cut(s) 354, 599
BstMBI GATC 5 cut(s) 130, 222, 292, 810, 1201
BstMWI GCNNNNNNNGC 5 cut(s) 676, 827, 832, 848, 1087
BstNI CCWGG 2 cut(s) 204, 1182
BstPI GGTNACC 1 cut(s) 140
BstSCI CCNGG 2 cut(s) 202, 1180
BstSFI CTRYAG 1 cut(s) 1141
BstUI CGCG 1 cut(s) 821
BstV1I GCAGC 1 cut(s) 829
BstV2I GAAGAC 1 cut(s) 1143
Bsu36I CCTNAGG 1 cut(s) 648
BsuI GTATCC 1 cut(s) 882
BsuRI GGCC 4 cut(s) 274, 339, 957, 993
BtsCI GGATG 1 cut(s) 1114
Cac8I GCNNGC 2 cut(s) 828, 1051
CaiI CAGNNNCTG 1 cut(s) 602
CfoI GCGC 4 cut(s) 821, 837, 1000, 1103
Cfr10I RCCGGY 2 cut(s) 532, 615
Cfr13I GGNCC 2 cut(s) 200, 526
CseI GACGC 1 cut(s) 810
Csp6I GTAC 3 cut(s) 430, 510, 690
CspAI ACCGGT 1 cut(s) 532
CspCI CAANNNNNGTGG 2 cut(s) 1167, 1202
CviAII CATG 7 cut(s) 181, 439, 762, 780, 859, 995, 1120
CviQI GTAC 3 cut(s) 430, 510, 690
DdeI CTNAG 7 cut(s) 266, 341, 447, 603, 648, 884, 1011
DpnI GATC 5 cut(s) 132, 224, 294, 812, 1203
DpnII GATC 5 cut(s) 130, 222, 292, 810, 1201
EaeI YGGCCR 1 cut(s) 991
Eam1104I CTCTTC 2 cut(s) 69, 1118
EarI CTCTTC 2 cut(s) 69, 1118
EciI GGCGGA 1 cut(s) 619
Eco130I CCWWGG 3 cut(s) 846, 878, 1205
Eco147I AGGCCT 1 cut(s) 274
Eco24I GRGCYC 1 cut(s) 309
Eco31I GGTCTC 1 cut(s) 599
Eco47I GGWCC 2 cut(s) 200, 526
Eco57I CTGAAG 1 cut(s) 1062
Eco81I CCTNAGG 1 cut(s) 648
Eco91I GGTNACC 1 cut(s) 140
EcoO65I GGTNACC 1 cut(s) 140
EcoRI GAATTC 1 cut(s) 1211
EcoRII CCWGG 2 cut(s) 202, 1180
EcoT14I CCWWGG 3 cut(s) 846, 878, 1205
EcoT38I GRGCYC 1 cut(s) 309
ErhI CCWWGG 3 cut(s) 846, 878, 1205
FaeI CATG 7 cut(s) 184, 442, 765, 783, 862, 998, 1123
FaqI GGGAC 2 cut(s) 689, 1175
FatI CATG 7 cut(s) 180, 438, 761, 779, 858, 994, 1119
FauI CCCGC 1 cut(s) 759
Fnu4HI GCNGC 3 cut(s) 620, 843, 1091
FokI GGATG 1 cut(s) 1121
FriOI GRGCYC 1 cut(s) 309
Fsp4HI GCNGC 3 cut(s) 620, 843, 1091
FspAI RTGCGCAY 1 cut(s) 999
FspBI CTAG 5 cut(s) 80, 276, 1050, 1157, 1216
FspI TGCGCA 2 cut(s) 999, 1102
GlaI GCGC 4 cut(s) 820, 836, 999, 1102
GluI GCNGC 3 cut(s) 620, 843, 1091
HaeII RGCGCY 1 cut(s) 838
HaeIII GGCC 4 cut(s) 274, 339, 957, 993
HapII CCGG 3 cut(s) 533, 616, 755
HgaI GACGC 1 cut(s) 810
HhaI GCGC 4 cut(s) 821, 837, 1000, 1103
Hin1II CATG 7 cut(s) 184, 442, 765, 783, 862, 998, 1123
Hin6I GCGC 4 cut(s) 819, 835, 998, 1101
HinP1I GCGC 4 cut(s) 819, 835, 998, 1101
HincII GTYRAC 2 cut(s) 260, 391
HindII GTYRAC 2 cut(s) 260, 391
HinfI GANTC 4 cut(s) 156, 287, 549, 758
HpaI GTTAAC 1 cut(s) 391
HpaII CCGG 3 cut(s) 533, 616, 755
HphI GGTGA 7 cut(s) 122, 152, 472, 551, 565, 580, 1048
Hpy166II GTNNAC 5 cut(s) 260, 391, 480, 723, 865
Hpy188I TCNGA 5 cut(s) 161, 269, 292, 381, 748
Hpy188III TCNNGA 1 cut(s) 650
Hpy8I GTNNAC 5 cut(s) 260, 391, 480, 723, 865
Hpy99I CGWCG 1 cut(s) 826
HpyAV CCTTC 7 cut(s) 81, 180, 184, 264, 363, 474, 525
HpyCH4III ACNGT 2 cut(s) 788, 1062
HpyCH4IV ACGT 1 cut(s) 692
HpyCH4V TGCA 8 cut(s) 237, 315, 425, 492, 670, 779, 830, 1119
HpyF10VI GCNNNNNNNGC 5 cut(s) 676, 827, 832, 848, 1087
HpyF3I CTNAG 7 cut(s) 266, 341, 447, 603, 648, 884, 1011
HpySE526I ACGT 1 cut(s) 692
Hsp92II CATG 7 cut(s) 184, 442, 765, 783, 862, 998, 1123
HspAI GCGC 4 cut(s) 819, 835, 998, 1101
KspAI GTTAAC 1 cut(s) 391
Kzo9I GATC 5 cut(s) 130, 222, 292, 810, 1201
LguI GCTCTTC 1 cut(s) 69
Lsp1109I GCAGC 1 cut(s) 829
LweI GCATC 1 cut(s) 246
MaeI CTAG 5 cut(s) 80, 276, 1050, 1157, 1216
MaeII ACGT 1 cut(s) 692
MaeIII GTNAC 4 cut(s) 117, 140, 1036, 1152
MalI GATC 5 cut(s) 132, 224, 294, 812, 1203
MboI GATC 5 cut(s) 130, 222, 292, 810, 1201
MboII GAAGA 6 cut(s) 17, 86, 296, 426, 1135, 1148
MfeI CAATTG 1 cut(s) 623
MhlI GDGCHC 1 cut(s) 309
MlsI TGGCCA 1 cut(s) 993
MluCI AATT 7 cut(s) 195, 503, 623, 663, 1016, 1191, 1211
MluNI TGGCCA 1 cut(s) 993
MlyI GAGTC 2 cut(s) 558, 767
MmeI TCCRAC 3 cut(s) 24, 553, 786
Mox20I TGGCCA 1 cut(s) 993
MroXI GAANNNNTTC 1 cut(s) 408
MscI TGGCCA 1 cut(s) 993
MseI TTAA 2 cut(s) 297, 390
MslI CAYNNNNRTG 1 cut(s) 185
Msp20I TGGCCA 1 cut(s) 993
MspI CCGG 3 cut(s) 533, 616, 755
MspR9I CCNGG 2 cut(s) 204, 1182
MunI CAATTG 1 cut(s) 623
MvaI CCWGG 2 cut(s) 204, 1182
MvnI CGCG 1 cut(s) 821
MwoI GCNNNNNNNGC 5 cut(s) 676, 827, 832, 848, 1087
NdeII GATC 5 cut(s) 130, 222, 292, 810, 1201
NheI GCTAGC 1 cut(s) 1049
NlaIII CATG 7 cut(s) 184, 442, 765, 783, 862, 998, 1123
NlaIV GGNNCC 1 cut(s) 900
NmuCI GTSAC 4 cut(s) 117, 140, 1036, 1152
NsbI TGCGCA 2 cut(s) 999, 1102
NspV TTCGAA 1 cut(s) 412
PceI AGGCCT 1 cut(s) 274
PciSI GCTCTTC 1 cut(s) 69
PdmI GAANNNNTTC 1 cut(s) 408
PfeI GAWTC 2 cut(s) 156, 287
PfoI TCCNGGA 1 cut(s) 202
PinAI ACCGGT 1 cut(s) 532
PkrI GCNGC 3 cut(s) 621, 844, 1092
PleI GAGTC 2 cut(s) 557, 766
PpsI GAGTC 2 cut(s) 557, 766
Psp6I CCWGG 2 cut(s) 202, 1180
PspEI GGTNACC 1 cut(s) 140
PspGI CCWGG 2 cut(s) 202, 1180
PspN4I GGNNCC 1 cut(s) 900
PspPI GGNCC 2 cut(s) 200, 526
PstNI CAGNNNCTG 1 cut(s) 602
RsaI GTAC 3 cut(s) 431, 511, 691
RsaNI GTAC 3 cut(s) 430, 510, 690
RseI CAYNNNNRTG 1 cut(s) 185
SapI GCTCTTC 1 cut(s) 69
SaqAI TTAA 2 cut(s) 297, 390
SatI GCNGC 3 cut(s) 620, 843, 1091
Sau3AI GATC 5 cut(s) 130, 222, 292, 810, 1201
Sau96I GGNCC 2 cut(s) 200, 526
ScaI AGTACT 1 cut(s) 431
SchI GAGTC 2 cut(s) 558, 767
ScrFI CCNGG 2 cut(s) 204, 1182
SduI GDGCHC 1 cut(s) 309
SfaNI GCATC 1 cut(s) 246
SfcI CTRYAG 1 cut(s) 1141
SfuI TTCGAA 1 cut(s) 412
SinI GGWCC 2 cut(s) 200, 526
SmiMI CAYNNNNRTG 1 cut(s) 185
Sse9I AATT 7 cut(s) 195, 503, 623, 663, 1016, 1191, 1211
SseBI AGGCCT 1 cut(s) 274
SsiI CCGC 6 cut(s) 562, 619, 630, 752, 1079, 1090
SspI AATATT 1 cut(s) 522
SspMI CTAG 5 cut(s) 80, 276, 1050, 1157, 1216
StuI AGGCCT 1 cut(s) 274
StyD4I CCNGG 2 cut(s) 202, 1180
StyI CCWWGG 3 cut(s) 846, 878, 1205
TaaI ACNGT 2 cut(s) 788, 1062
TaiI ACGT 1 cut(s) 695
TaqI TCGA 5 cut(s) 154, 303, 412, 547, 1169
TasI AATT 7 cut(s) 195, 503, 623, 663, 1016, 1191, 1211
TatI WGTACW 1 cut(s) 429
TauI GCSGC 2 cut(s) 622, 1093
TfiI GAWTC 2 cut(s) 156, 287
Tru1I TTAA 2 cut(s) 297, 390
Tru9I TTAA 2 cut(s) 297, 390
TseFI GTSAC 4 cut(s) 117, 140, 1036, 1152
TseI GCWGC 1 cut(s) 842
Tsp45I GTSAC 4 cut(s) 117, 140, 1036, 1152
TspDTI ATGAA 2 cut(s) 297, 1136
VpaK11BI GGWCC 2 cut(s) 200, 526
XapI RAATTY 2 cut(s) 503, 1211
XmnI GAANNNNTTC 1 cut(s) 408
XspI CTAG 5 cut(s) 80, 276, 1050, 1157, 1216
ZrmI AGTACT 1 cut(s) 431
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.