Rh6DG047300

N-acetyltransferase HLS1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0004042
Physical Location & Seq
Forward (+)
5013740 .. 5015282
1543 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG047300.1

Sequence Viewer

Length: 1200 bp
ATGAGAGCCAATATGTTAGTGATTAGAGAGCTGGACTTGAACAAGGACACACCAAGAGTTGAAGAGTTAGAGAGAGGGTGTGAGGCTGGCTCAAGTGGCGAAATGTCACTCTTTACTGATCTTTTGGGTGACCCAATTTGCCGAATCCGAAACTCGCCCTCGTTCCTCATGCTGGTGGCTGAAATGGGAAAAGAAATCGTTGGGATCATCAGGGGCTGCATCAAAGTCGTTTCTTGTGGTAAAAACATCACGAGGCCTAGCAATGAAGATACCGATCTTAAATTCAAACCCATTTGCACCAAACTTGCTTACATTCTTGGGCTTAGAGTTTCTGCTACACACAGGAGAAAAGGTATTGGTCTGAAACTAGTTAACAGAATGGAGGAATGGTTTCGAAGAAATGGTGCAGAGTACTCATACATGGCTACTGAGCTTGACAATAAAGCTTCACTAAACCTTTTCACCCAAAACTGCTACTACACCAAATTTCGTACCCCTTCAATTTTGGTCCAACCGGTCTTTGCTCACCGAGTCAAAACTCACCGCAGCCGAGTCACCATTATTGAACTCACCCCACCAGAGGCTGAGACCCTCTACCGCCACCAATTCTCCGCCACCGAGTTTTTTCCTCAGGACATTGATAGAATTGTGGAAAACAAGCTCAATGTTGGTACATTTTTAGCTGTCCCAACTGGATTTTCGTGGATGGGTTTGGACAGGTTTCTATCCAAGCCGCCCGAGTCATGGGCTGTTTTGAGTGCATGGAATTGTAAGGAGGCGTTTACATTGGAAATCAAAGGTGCATCGCTTGCACGGCGCTTGGCTGCCAAGGCAACTCGCATGGTGGATGGGGCGTTGCCTTGGCTGAGGATACCCTCGGTCCCCAACTTTTTTAGGCCATTTGGGTTGCTTTTCCTGTATGGGTTAGGAGGACAAGGCCCACGCGCCGATAAGATGGCAAAGGTGCTTTGTGGCCATGCACATAACGTAGCTAAGGAATTGGGCTGTGGGGTGGTGGTGACTGAAGTAGCCAGCCAAGAACCGTTGAGGTTAGCAGTACCACATTGGAAGCGGCTATCGTGCGCCGAGGATGTGTGGTGCATCAAGAGGCTTGTTGAGGACTATAGTGATGGGTCACTTGGGGACTGGACCAAATCTCCTCCCGGGCTATCAATTTTTGTTGATCCTAGGGAATTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Acetyltransf_1

Protein Analysis

399

Amino Acids

44.9

Weight (kDa)

9.05

Isoelectric Point (pI)

39.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Acetyltransf_1 PF00583 50 - 156 8.3e-13 Acetyltransferase (GNAT) family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 945
AciI CCGC 5 cut(s) 544, 598, 612, 734, 1072
AclWI GGATC 2 cut(s) 212, 1178
AcoI YGGCCR 1 cut(s) 973
AcsI RAATTY 3 cut(s) 281, 485, 1193
AcuI CTGAAG 1 cut(s) 1044
AfaI GTAC 4 cut(s) 413, 493, 673, 1059
AfiI CCNNNNNNNGG 3 cut(s) 172, 580, 744
AgeI ACCGGT 1 cut(s) 514
AgsI TTSAA 5 cut(s) 40, 62, 286, 501, 566
AhlI ACTAGT 1 cut(s) 367
AjuI GAANNNNNNNTTGG 2 cut(s) 682, 714
AluBI AGCT 6 cut(s) 31, 433, 446, 661, 683, 992
AluI AGCT 6 cut(s) 31, 433, 446, 661, 683, 992
Alw26I GTCTC 1 cut(s) 581
AlwI GGATC 2 cut(s) 212, 1178
AlwNI CAGNNNCTG 2 cut(s) 216, 584
Ama87I CYCGRG 2 cut(s) 737, 1163
AoxI GGCC 4 cut(s) 254, 896, 937, 973
ApeKI GCWGC 3 cut(s) 216, 546, 824
ApoI RAATTY 3 cut(s) 281, 485, 1193
AsiGI ACCGGT 1 cut(s) 514
Asp700I GAANNNNTTC 1 cut(s) 390
AspA2I CCTAGG 1 cut(s) 1187
AspLEI GCGC 3 cut(s) 819, 947, 1085
AspS9I GGNCC 4 cut(s) 508, 880, 938, 1149
AsuC2I CCSGG 2 cut(s) 1164, 1165
AsuHPI GGTGA 7 cut(s) 140, 454, 518, 533, 547, 562, 1030
AsuII TTCGAA 1 cut(s) 394
AvaI CYCGRG 2 cut(s) 737, 1163
AvaII GGWCC 3 cut(s) 508, 880, 1149
AvrII CCTAGG 1 cut(s) 1187
AxyI CCTNAGG 1 cut(s) 630
BalI TGGCCA 1 cut(s) 975
BauI CACGAG 1 cut(s) 250
BbvCI CCTCAGC 1 cut(s) 866
BbvI GCAGC 3 cut(s) 203, 558, 811
BccI CCATC 4 cut(s) 700, 842, 949, 1124
BceAI ACGGC 1 cut(s) 830
BcgI CGANNNNNNTGC 2 cut(s) 208, 242
BciVI GTATCC 1 cut(s) 864
BcnI CCSGG 2 cut(s) 1164, 1165
BcoDI GTCTC 1 cut(s) 581
BcuI ACTAGT 1 cut(s) 367
BfaI CTAG 4 cut(s) 258, 368, 1188, 1198
BfmI CTRYAG 1 cut(s) 1123
BfoI RGCGCY 1 cut(s) 820
BfuI GTATCC 1 cut(s) 864
BisI GCNGC 5 cut(s) 217, 547, 734, 825, 1073
BlnI CCTAGG 1 cut(s) 1187
BlsI GCNGC 5 cut(s) 218, 548, 735, 826, 1074
BmcAI AGTACT 1 cut(s) 413
Bme1390I CCNGG 2 cut(s) 1164, 1165
Bme18I GGWCC 3 cut(s) 508, 880, 1149
BmeT110I CYCGRG 2 cut(s) 737, 1163
BmgT120I GGNCC 4 cut(s) 508, 880, 938, 1149
BmiI GGNNCC 1 cut(s) 882
BmrFI CCNGG 2 cut(s) 1164, 1165
BmsI GCATC 3 cut(s) 228, 812, 1110
BplI GAGNNNNNCTC 2 cut(s) 74, 106
Bpu10I CCTNAGC 2 cut(s) 866, 993
Bpu14I TTCGAA 1 cut(s) 394
BpuEI CTTGAG 1 cut(s) 76
BpuMI CCSGG 2 cut(s) 1164, 1165
BsaBI GATNNNNATC 1 cut(s) 273
BsaI GGTCTC 1 cut(s) 581
BsaJI CCNNGG 6 cut(s) 828, 860, 876, 1086, 1163, 1187
BsaWI WCCGGW 1 cut(s) 514
BsaXI ACNNNNNCTCC 8 cut(s) 337, 367, 374, 404, 593, 623, 1141, 1171
Bsc4I CCNNNNNNNGG 3 cut(s) 172, 580, 744
Bse118I RCCGGY 1 cut(s) 514
Bse1I ACTGG 2 cut(s) 697, 1151
Bse21I CCTNAGG 1 cut(s) 630
Bse3DI GCAATG 1 cut(s) 268
Bse8I GATNNNNATC 1 cut(s) 273
BseDI CCNNGG 6 cut(s) 828, 860, 876, 1086, 1163, 1187
BseGI GGATG 3 cut(s) 711, 853, 1096
BseJI GATNNNNATC 1 cut(s) 273
BseLI CCNNNNNNNGG 3 cut(s) 172, 580, 744
BseMI GCAATG 1 cut(s) 268
BseMII CTCAG 4 cut(s) 420, 576, 644, 857
BseNI ACTGG 2 cut(s) 697, 1151
BseRI GAGGAG 1 cut(s) 1149
BseXI GCAGC 3 cut(s) 203, 558, 811
BsgI GTGCAG 1 cut(s) 426
Bsh1236I CGCG 1 cut(s) 945
BshFI GGCC 4 cut(s) 256, 898, 939, 975
BshTI ACCGGT 1 cut(s) 514
BsiHKCI CYCGRG 2 cut(s) 737, 1163
BsiSI CCGG 2 cut(s) 515, 1164
BslFI GGGAC 3 cut(s) 671, 866, 1157
BslI CCNNNNNNNGG 3 cut(s) 172, 580, 744
BsmAI GTCTC 1 cut(s) 581
BsmFI GGGAC 3 cut(s) 671, 866, 1157
BsnI GGCC 4 cut(s) 256, 898, 939, 975
Bso31I GGTCTC 1 cut(s) 581
BsoBI CYCGRG 2 cut(s) 737, 1163
Bsp119I TTCGAA 1 cut(s) 394
Bsp143I GATC 4 cut(s) 118, 204, 274, 1183
BspACI CCGC 5 cut(s) 544, 598, 612, 734, 1072
BspANI GGCC 4 cut(s) 256, 898, 939, 975
BspCNI CTCAG 4 cut(s) 421, 577, 643, 858
BspFNI CGCG 1 cut(s) 945
BspLI GGNNCC 1 cut(s) 882
BspPI GGATC 2 cut(s) 212, 1178
BspT104I TTCGAA 1 cut(s) 394
BspTNI GGTCTC 1 cut(s) 581
BsrDI GCAATG 1 cut(s) 268
BsrFI RCCGGY 1 cut(s) 514
BsrI ACTGG 2 cut(s) 697, 1151
BssAI RCCGGY 1 cut(s) 514
BssECI CCNNGG 6 cut(s) 828, 860, 876, 1086, 1163, 1187
BssMI GATC 4 cut(s) 118, 204, 274, 1183
BssSI CACGAG 1 cut(s) 250
BssT1I CCWWGG 3 cut(s) 828, 860, 1187
Bst2BI CACGAG 1 cut(s) 250
Bst4CI ACNGT 1 cut(s) 1044
Bst6I CTCTTC 1 cut(s) 57
BstAPI GCANNNNNTGC 1 cut(s) 809
BstBI TTCGAA 1 cut(s) 394
BstC8I GCNNGC 3 cut(s) 88, 810, 1033
BstDEI CTNAG 6 cut(s) 323, 429, 585, 630, 866, 993
BstEII GGTNACC 1 cut(s) 128
BstF5I GGATG 3 cut(s) 711, 853, 1096
BstFNI CGCG 1 cut(s) 945
BstH2I RGCGCY 1 cut(s) 820
BstHHI GCGC 3 cut(s) 819, 947, 1085
BstKTI GATC 4 cut(s) 121, 207, 277, 1186
BstMAI GTCTC 1 cut(s) 581
BstMBI GATC 4 cut(s) 118, 204, 274, 1183
BstMWI GCNNNNNNNGC 4 cut(s) 96, 809, 814, 830
BstPI GGTNACC 1 cut(s) 128
BstSCI CCNGG 2 cut(s) 1162, 1163
BstSFI CTRYAG 1 cut(s) 1123
BstUI CGCG 1 cut(s) 945
BstV1I GCAGC 3 cut(s) 203, 558, 811
Bsu36I CCTNAGG 1 cut(s) 630
BsuI GTATCC 1 cut(s) 864
BsuRI GGCC 4 cut(s) 256, 898, 939, 975
BtgZI GCGATG 1 cut(s) 789
BtsCI GGATG 3 cut(s) 711, 853, 1096
Cac8I GCNNGC 3 cut(s) 88, 810, 1033
CaiI CAGNNNCTG 2 cut(s) 216, 584
CfoI GCGC 3 cut(s) 819, 947, 1085
Cfr10I RCCGGY 1 cut(s) 514
Cfr13I GGNCC 4 cut(s) 508, 880, 938, 1149
Cfr9I CCCGGG 1 cut(s) 1163
Csp6I GTAC 4 cut(s) 412, 492, 672, 1058
CspAI ACCGGT 1 cut(s) 514
CviAII CATG 6 cut(s) 169, 421, 744, 762, 841, 977
CviQI GTAC 4 cut(s) 412, 492, 672, 1058
DdeI CTNAG 6 cut(s) 323, 429, 585, 630, 866, 993
DpnI GATC 4 cut(s) 120, 206, 276, 1185
DpnII GATC 4 cut(s) 118, 204, 274, 1183
EaeI YGGCCR 1 cut(s) 973
Eam1104I CTCTTC 1 cut(s) 57
EarI CTCTTC 1 cut(s) 57
EciI GGCGGA 1 cut(s) 601
Eco130I CCWWGG 3 cut(s) 828, 860, 1187
Eco147I AGGCCT 1 cut(s) 256
Eco31I GGTCTC 1 cut(s) 581
Eco47I GGWCC 3 cut(s) 508, 880, 1149
Eco57I CTGAAG 1 cut(s) 1044
Eco81I CCTNAGG 1 cut(s) 630
Eco88I CYCGRG 2 cut(s) 737, 1163
Eco91I GGTNACC 1 cut(s) 128
EcoO65I GGTNACC 1 cut(s) 128
EcoRI GAATTC 1 cut(s) 1193
EcoT14I CCWWGG 3 cut(s) 828, 860, 1187
ErhI CCWWGG 3 cut(s) 828, 860, 1187
FaeI CATG 6 cut(s) 172, 424, 747, 765, 844, 980
FaqI GGGAC 3 cut(s) 671, 866, 1157
FatI CATG 6 cut(s) 168, 420, 743, 761, 840, 976
Fnu4HI GCNGC 5 cut(s) 217, 547, 734, 825, 1073
FokI GGATG 3 cut(s) 718, 860, 1103
Fsp4HI GCNGC 5 cut(s) 217, 547, 734, 825, 1073
FspBI CTAG 4 cut(s) 258, 368, 1188, 1198
GlaI GCGC 3 cut(s) 818, 946, 1084
GluI GCNGC 5 cut(s) 217, 547, 734, 825, 1073
HaeII RGCGCY 1 cut(s) 820
HaeIII GGCC 4 cut(s) 256, 898, 939, 975
HapII CCGG 2 cut(s) 515, 1164
HhaI GCGC 3 cut(s) 819, 947, 1085
Hin1II CATG 6 cut(s) 172, 424, 747, 765, 844, 980
Hin6I GCGC 3 cut(s) 817, 945, 1083
HinP1I GCGC 3 cut(s) 817, 945, 1083
HincII GTYRAC 1 cut(s) 373
HindII GTYRAC 1 cut(s) 373
HindIII AAGCTT 1 cut(s) 444
HinfI GANTC 4 cut(s) 144, 531, 552, 740
HpaI GTTAAC 1 cut(s) 373
HpaII CCGG 2 cut(s) 515, 1164
HphI GGTGA 7 cut(s) 140, 454, 518, 533, 547, 562, 1030
Hpy166II GTNNAC 2 cut(s) 373, 783
Hpy188I TCNGA 2 cut(s) 149, 363
Hpy188III TCNNGA 3 cut(s) 250, 632, 1105
Hpy8I GTNNAC 2 cut(s) 373, 783
HpyAV CCTTC 1 cut(s) 507
HpyCH4III ACNGT 1 cut(s) 1044
HpyCH4IV ACGT 1 cut(s) 987
HpyCH4V TGCA 8 cut(s) 219, 297, 407, 761, 803, 812, 980, 1101
HpyF10VI GCNNNNNNNGC 4 cut(s) 96, 809, 814, 830
HpyF3I CTNAG 6 cut(s) 323, 429, 585, 630, 866, 993
HpySE526I ACGT 1 cut(s) 987
Hsp92II CATG 6 cut(s) 172, 424, 747, 765, 844, 980
HspAI GCGC 3 cut(s) 817, 945, 1083
KspAI GTTAAC 1 cut(s) 373
Kzo9I GATC 4 cut(s) 118, 204, 274, 1183
Lsp1109I GCAGC 3 cut(s) 203, 558, 811
LweI GCATC 3 cut(s) 228, 812, 1110
MaeI CTAG 4 cut(s) 258, 368, 1188, 1198
MaeII ACGT 1 cut(s) 987
MaeIII GTNAC 5 cut(s) 105, 128, 553, 1018, 1134
MalI GATC 4 cut(s) 120, 206, 276, 1185
MboI GATC 4 cut(s) 118, 204, 274, 1183
MboII GAAGA 3 cut(s) 74, 278, 408
MlsI TGGCCA 1 cut(s) 975
MluNI TGGCCA 1 cut(s) 975
MlyI GAGTC 3 cut(s) 540, 561, 749
MmeI TCCRAC 1 cut(s) 535
Mox20I TGGCCA 1 cut(s) 975
MroXI GAANNNNTTC 1 cut(s) 390
MscI TGGCCA 1 cut(s) 975
MseI TTAA 2 cut(s) 279, 372
MslI CAYNNNNRTG 1 cut(s) 173
Msp20I TGGCCA 1 cut(s) 975
MspI CCGG 2 cut(s) 515, 1164
MspR9I CCNGG 2 cut(s) 1164, 1165
MvnI CGCG 1 cut(s) 945
MwoI GCNNNNNNNGC 4 cut(s) 96, 809, 814, 830
NciI CCSGG 2 cut(s) 1164, 1165
NdeII GATC 4 cut(s) 118, 204, 274, 1183
NlaIII CATG 6 cut(s) 172, 424, 747, 765, 844, 980
NlaIV GGNNCC 1 cut(s) 882
NmeAIII GCCGAG 2 cut(s) 575, 1111
NmuCI GTSAC 5 cut(s) 105, 128, 553, 1018, 1134
NspV TTCGAA 1 cut(s) 394
PceI AGGCCT 1 cut(s) 256
PdmI GAANNNNTTC 1 cut(s) 390
PfeI GAWTC 1 cut(s) 144
PinAI ACCGGT 1 cut(s) 514
PkrI GCNGC 5 cut(s) 218, 548, 735, 826, 1074
PleI GAGTC 3 cut(s) 539, 560, 748
PpsI GAGTC 3 cut(s) 539, 560, 748
PspEI GGTNACC 1 cut(s) 128
PspN4I GGNNCC 1 cut(s) 882
PspPI GGNCC 4 cut(s) 508, 880, 938, 1149
PstNI CAGNNNCTG 2 cut(s) 216, 584
RsaI GTAC 4 cut(s) 413, 493, 673, 1059
RsaNI GTAC 4 cut(s) 412, 492, 672, 1058
RseI CAYNNNNRTG 1 cut(s) 173
SaqAI TTAA 2 cut(s) 279, 372
SatI GCNGC 5 cut(s) 217, 547, 734, 825, 1073
Sau3AI GATC 4 cut(s) 118, 204, 274, 1183
Sau96I GGNCC 4 cut(s) 508, 880, 938, 1149
ScaI AGTACT 1 cut(s) 413
SchI GAGTC 3 cut(s) 540, 561, 749
ScrFI CCNGG 2 cut(s) 1164, 1165
SfaNI GCATC 3 cut(s) 228, 812, 1110
SfcI CTRYAG 1 cut(s) 1123
SfuI TTCGAA 1 cut(s) 394
SinI GGWCC 3 cut(s) 508, 880, 1149
SmaI CCCGGG 1 cut(s) 1165
SmiMI CAYNNNNRTG 1 cut(s) 173
SmlI CTYRAG 1 cut(s) 91
SmoI CTYRAG 1 cut(s) 91
SpeI ACTAGT 1 cut(s) 367
SseBI AGGCCT 1 cut(s) 256
SsiI CCGC 5 cut(s) 544, 598, 612, 734, 1072
SspMI CTAG 4 cut(s) 258, 368, 1188, 1198
StuI AGGCCT 1 cut(s) 256
StyD4I CCNGG 2 cut(s) 1162, 1163
StyI CCWWGG 3 cut(s) 828, 860, 1187
TaaI ACNGT 1 cut(s) 1044
TaiI ACGT 1 cut(s) 990
TaqI TCGA 1 cut(s) 394
TaqII GACCGA 1 cut(s) 868
TatI WGTACW 1 cut(s) 411
TauI GCSGC 2 cut(s) 736, 1075
TfiI GAWTC 1 cut(s) 144
Tru1I TTAA 2 cut(s) 279, 372
Tru9I TTAA 2 cut(s) 279, 372
TseFI GTSAC 5 cut(s) 105, 128, 553, 1018, 1134
TseI GCWGC 3 cut(s) 216, 546, 824
Tsp45I GTSAC 5 cut(s) 105, 128, 553, 1018, 1134
TspDTI ATGAA 1 cut(s) 279
TspMI CCCGGG 1 cut(s) 1163
VpaK11BI GGWCC 3 cut(s) 508, 880, 1149
XapI RAATTY 3 cut(s) 281, 485, 1193
XmaI CCCGGG 1 cut(s) 1163
XmaJI CCTAGG 1 cut(s) 1187
XmnI GAANNNNTTC 1 cut(s) 390
XspI CTAG 4 cut(s) 258, 368, 1188, 1198
ZrmI AGTACT 1 cut(s) 413
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.