RchiOBHm_Chr6g0253471

N-acetyltransferase HLS1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0004042
Physical Location & Seq
Forward (+)
8573823 .. 8575352
1530 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ22729

Sequence Viewer

Length: 1200 bp
ATGAGAGCCAATATATTAGTGATTAGAGAGCTGGACTCGAAAAAGGACACACCAAGAGTCGAAGAGTTAGAGAGAGGGTGTGAGGCTGGCTCAAGTGGCGAAATGTCACTCTTTACTGATCTTTTGGGTGACCCGATTTGCCGAATCCGAAACTCGCCCTCGTTCCTCATGCTGGTGGCTGAAATGGGAAAAGAAATCGTTGGGATCATCAGGGGTTGCATCAAAGTCGTTTCTTGTGGTAAAAACATCACGAGGCCTAGCAATGAAGATTCCGATCTTAAATTCAAACCCATTTGCACCAAACTTGCTTACATTCTTGGCCTTAGAGTTTCCGCTTCACACAGGAGAAAAGGTATTGGTCTGAAACTAGTTAACAGAATGGAGGAATGGTTTCGAAGAAATGGTGCAGAGTACTCATACATGGCTACTGAGCTTGACAATAAAGCTTCACTAAATCTTTTCACCCAAAAATGCTACTACACCAAATTTCGTACCCCTTCAATTTTGGTCCAACCAGTCTTTGCTCACCGAGTCAAAACTCACCGCAACCGAGTCACCATTATTGAACTCACCCCACCAGAGGCTGAGACCCTCTACCGCCGCCAATTCTCTGCCACCGAGTTTTTTCCTCAGGACATTGATACAATTGTGGAAAACAAGCTCAATGTAGGTACATTTTTAGCTGTCCCAACCGAGTTTTTGTGGACGGGTTTGGAAAGGTTTCTATCCAAGCCGCCCGAGTCATGGGCTGTTTTGAGTGCATGGAATTGTAAGGAGGTGTTTACATTGGAAATCAAAGGTGCATCGCTTGCACGGCGCTTGGCTGCCGGGGCAACTCGCATGGTGGATAGGGCGCTGCCTTGGCTGAGGATACCTTCGGTCCCCAATATTTTTAGGCCATTTGGGTTGCTTTTCCTGTATGGGTTAGGAGGACAAGGCCCACGCGCAGATAAGCTGGCAAAGGTGCTCTGTGGCCATGCACATAACGTAGCTAGGGAATTGGGCTGTGGGGTGGTGGTGACTGAAGTAGCCAACCAAGAACCGTTGAGGTTAGCAGTACCACATTGGAAGCGTCTATCGTGCGCCGAGGATGTGTGGTGCATCAAGAGGCTTGTTGAGGACTATAGTGATGGGTCACTTGGGGACTGGACCAAATCTCCACCCGGGTTATCAATTTTTGTTGATCCAAGGGAATTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Acetyltransf_1

Protein Analysis

399

Amino Acids

44.99

Weight (kDa)

9.13

Isoelectric Point (pI)

41.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Acetyltransf_1 PF00583 50 - 156 3.5e-13 Acetyltransferase (GNAT) family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 945
AciI CCGC 5 cut(s) 333, 544, 598, 601, 734
AclWI GGATC 2 cut(s) 212, 1178
AcoI YGGCCR 1 cut(s) 973
AcsI RAATTY 3 cut(s) 281, 485, 1193
AcuI CTGAAG 1 cut(s) 1044
AfaI GTAC 4 cut(s) 413, 493, 673, 1059
AfiI CCNNNNNNNGG 3 cut(s) 172, 580, 744
AgsI TTSAA 3 cut(s) 286, 501, 566
AhlI ACTAGT 1 cut(s) 367
AluBI AGCT 7 cut(s) 31, 433, 446, 661, 683, 955, 992
AluI AGCT 7 cut(s) 31, 433, 446, 661, 683, 955, 992
Alw21I GWGCWC 1 cut(s) 969
Alw26I GTCTC 1 cut(s) 581
AlwI GGATC 2 cut(s) 212, 1178
AlwNI CAGNNNCTG 1 cut(s) 584
Ama87I CYCGRG 2 cut(s) 737, 1163
AoxI GGCC 5 cut(s) 254, 319, 896, 937, 973
ApeKI GCWGC 2 cut(s) 824, 856
ApoI RAATTY 3 cut(s) 281, 485, 1193
Asp700I GAANNNNTTC 2 cut(s) 390, 720
AspLEI GCGC 4 cut(s) 819, 856, 947, 1085
AspS9I GGNCC 4 cut(s) 508, 880, 938, 1149
AsuC2I CCSGG 3 cut(s) 829, 1164, 1165
AsuHPI GGTGA 7 cut(s) 140, 454, 518, 533, 547, 562, 1030
AsuII TTCGAA 1 cut(s) 394
AvaI CYCGRG 2 cut(s) 737, 1163
AvaII GGWCC 3 cut(s) 508, 880, 1149
AxyI CCTNAGG 1 cut(s) 630
BaeI ACNNNNGTAYC 2 cut(s) 633, 666
BalI TGGCCA 1 cut(s) 975
BauI CACGAG 1 cut(s) 250
Bbv12I GWGCWC 1 cut(s) 969
BbvCI CCTCAGC 1 cut(s) 866
BbvI GCAGC 2 cut(s) 811, 843
BccI CCATC 1 cut(s) 1124
BceAI ACGGC 1 cut(s) 830
BcgI CGANNNNNNTGC 2 cut(s) 208, 242
BciVI GTATCC 1 cut(s) 864
BcnI CCSGG 3 cut(s) 829, 1164, 1165
BcoDI GTCTC 1 cut(s) 581
BcuI ACTAGT 1 cut(s) 367
BfaI CTAG 4 cut(s) 258, 368, 993, 1198
BfmI CTRYAG 1 cut(s) 1123
BfoI RGCGCY 2 cut(s) 820, 857
BfuI GTATCC 1 cut(s) 864
BisI GCNGC 4 cut(s) 601, 734, 825, 857
BlsI GCNGC 4 cut(s) 602, 735, 826, 858
BmcAI AGTACT 1 cut(s) 413
Bme1390I CCNGG 3 cut(s) 829, 1164, 1165
Bme18I GGWCC 3 cut(s) 508, 880, 1149
BmeT110I CYCGRG 2 cut(s) 737, 1163
BmgT120I GGNCC 4 cut(s) 508, 880, 938, 1149
BmiI GGNNCC 1 cut(s) 882
BmrFI CCNGG 3 cut(s) 829, 1164, 1165
BmsI GCATC 3 cut(s) 228, 812, 1110
BplI GAGNNNNNCTC 4 cut(s) 20, 52, 74, 106
Bpu10I CCTNAGC 1 cut(s) 866
Bpu14I TTCGAA 1 cut(s) 394
BpuEI CTTGAG 1 cut(s) 76
BpuMI CCSGG 3 cut(s) 829, 1164, 1165
BsaBI GATNNNNATC 1 cut(s) 273
BsaI GGTCTC 1 cut(s) 581
BsaJI CCNNGG 5 cut(s) 828, 860, 1086, 1163, 1187
BsaXI ACNNNNNCTCC 6 cut(s) 337, 367, 374, 404, 1141, 1171
Bsc4I CCNNNNNNNGG 3 cut(s) 172, 580, 744
Bse1I ACTGG 2 cut(s) 515, 1151
Bse21I CCTNAGG 1 cut(s) 630
Bse3DI GCAATG 1 cut(s) 268
Bse8I GATNNNNATC 1 cut(s) 273
BseDI CCNNGG 5 cut(s) 828, 860, 1086, 1163, 1187
BseGI GGATG 1 cut(s) 1096
BseJI GATNNNNATC 1 cut(s) 273
BseLI CCNNNNNNNGG 3 cut(s) 172, 580, 744
BseMI GCAATG 1 cut(s) 268
BseMII CTCAG 4 cut(s) 420, 576, 644, 857
BseNI ACTGG 2 cut(s) 515, 1151
BseXI GCAGC 2 cut(s) 811, 843
BsgI GTGCAG 1 cut(s) 426
Bsh1236I CGCG 1 cut(s) 945
BshFI GGCC 5 cut(s) 256, 321, 898, 939, 975
BsiHKAI GWGCWC 1 cut(s) 969
BsiHKCI CYCGRG 2 cut(s) 737, 1163
BsiSI CCGG 2 cut(s) 828, 1164
BslFI GGGAC 3 cut(s) 671, 866, 1157
BslI CCNNNNNNNGG 3 cut(s) 172, 580, 744
BsmAI GTCTC 1 cut(s) 581
BsmFI GGGAC 3 cut(s) 671, 866, 1157
BsnI GGCC 5 cut(s) 256, 321, 898, 939, 975
Bso31I GGTCTC 1 cut(s) 581
BsoBI CYCGRG 2 cut(s) 737, 1163
Bsp119I TTCGAA 1 cut(s) 394
Bsp1286I GDGCHC 1 cut(s) 969
Bsp143I GATC 4 cut(s) 118, 204, 274, 1183
BspACI CCGC 5 cut(s) 333, 544, 598, 601, 734
BspANI GGCC 5 cut(s) 256, 321, 898, 939, 975
BspCNI CTCAG 4 cut(s) 421, 577, 643, 858
BspFNI CGCG 1 cut(s) 945
BspLI GGNNCC 1 cut(s) 882
BspPI GGATC 2 cut(s) 212, 1178
BspT104I TTCGAA 1 cut(s) 394
BspTNI GGTCTC 1 cut(s) 581
BsrDI GCAATG 1 cut(s) 268
BsrI ACTGG 2 cut(s) 515, 1151
BssECI CCNNGG 5 cut(s) 828, 860, 1086, 1163, 1187
BssMI GATC 4 cut(s) 118, 204, 274, 1183
BssSI CACGAG 1 cut(s) 250
BssT1I CCWWGG 2 cut(s) 860, 1187
Bst2BI CACGAG 1 cut(s) 250
Bst4CI ACNGT 1 cut(s) 1044
Bst6I CTCTTC 1 cut(s) 57
BstAPI GCANNNNNTGC 1 cut(s) 809
BstBI TTCGAA 1 cut(s) 394
BstC8I GCNNGC 3 cut(s) 88, 810, 957
BstDEI CTNAG 5 cut(s) 323, 429, 585, 630, 866
BstEII GGTNACC 1 cut(s) 128
BstF5I GGATG 1 cut(s) 1096
BstFNI CGCG 1 cut(s) 945
BstH2I RGCGCY 2 cut(s) 820, 857
BstHHI GCGC 4 cut(s) 819, 856, 947, 1085
BstKTI GATC 4 cut(s) 121, 207, 277, 1186
BstMAI GTCTC 1 cut(s) 581
BstMBI GATC 4 cut(s) 118, 204, 274, 1183
BstMWI GCNNNNNNNGC 5 cut(s) 96, 809, 814, 830, 862
BstPI GGTNACC 1 cut(s) 128
BstSCI CCNGG 3 cut(s) 827, 1162, 1163
BstSFI CTRYAG 1 cut(s) 1123
BstUI CGCG 1 cut(s) 945
BstV1I GCAGC 2 cut(s) 811, 843
Bsu36I CCTNAGG 1 cut(s) 630
BsuI GTATCC 1 cut(s) 864
BsuRI GGCC 5 cut(s) 256, 321, 898, 939, 975
BtgZI GCGATG 1 cut(s) 789
BtsCI GGATG 1 cut(s) 1096
Cac8I GCNNGC 3 cut(s) 88, 810, 957
CaiI CAGNNNCTG 1 cut(s) 584
CfoI GCGC 4 cut(s) 819, 856, 947, 1085
Cfr13I GGNCC 4 cut(s) 508, 880, 938, 1149
Cfr9I CCCGGG 1 cut(s) 1163
CseI GACGC 1 cut(s) 1061
Csp6I GTAC 4 cut(s) 412, 492, 672, 1058
CviAII CATG 6 cut(s) 169, 421, 744, 762, 841, 977
CviQI GTAC 4 cut(s) 412, 492, 672, 1058
DdeI CTNAG 5 cut(s) 323, 429, 585, 630, 866
DpnI GATC 4 cut(s) 120, 206, 276, 1185
DpnII GATC 4 cut(s) 118, 204, 274, 1183
EaeI YGGCCR 1 cut(s) 973
Eam1104I CTCTTC 1 cut(s) 57
EarI CTCTTC 1 cut(s) 57
Eco130I CCWWGG 2 cut(s) 860, 1187
Eco147I AGGCCT 1 cut(s) 256
Eco31I GGTCTC 1 cut(s) 581
Eco47I GGWCC 3 cut(s) 508, 880, 1149
Eco57I CTGAAG 1 cut(s) 1044
Eco81I CCTNAGG 1 cut(s) 630
Eco88I CYCGRG 2 cut(s) 737, 1163
Eco91I GGTNACC 1 cut(s) 128
EcoO65I GGTNACC 1 cut(s) 128
EcoRI GAATTC 1 cut(s) 1193
EcoT14I CCWWGG 2 cut(s) 860, 1187
ErhI CCWWGG 2 cut(s) 860, 1187
FaeI CATG 6 cut(s) 172, 424, 747, 765, 844, 980
FaqI GGGAC 3 cut(s) 671, 866, 1157
FatI CATG 6 cut(s) 168, 420, 743, 761, 840, 976
Fnu4HI GCNGC 4 cut(s) 601, 734, 825, 857
FokI GGATG 1 cut(s) 1103
Fsp4HI GCNGC 4 cut(s) 601, 734, 825, 857
FspBI CTAG 4 cut(s) 258, 368, 993, 1198
GlaI GCGC 4 cut(s) 818, 855, 946, 1084
GluI GCNGC 4 cut(s) 601, 734, 825, 857
HaeII RGCGCY 2 cut(s) 820, 857
HaeIII GGCC 5 cut(s) 256, 321, 898, 939, 975
HapII CCGG 2 cut(s) 828, 1164
HgaI GACGC 1 cut(s) 1061
HhaI GCGC 4 cut(s) 819, 856, 947, 1085
Hin1II CATG 6 cut(s) 172, 424, 747, 765, 844, 980
Hin6I GCGC 4 cut(s) 817, 854, 945, 1083
HinP1I GCGC 4 cut(s) 817, 854, 945, 1083
HincII GTYRAC 1 cut(s) 373
HindII GTYRAC 1 cut(s) 373
HindIII AAGCTT 1 cut(s) 444
HinfI GANTC 7 cut(s) 35, 57, 144, 269, 531, 552, 740
HpaI GTTAAC 1 cut(s) 373
HpaII CCGG 2 cut(s) 828, 1164
HphI GGTGA 7 cut(s) 140, 454, 518, 533, 547, 562, 1030
Hpy166II GTNNAC 3 cut(s) 373, 705, 783
Hpy188I TCNGA 3 cut(s) 149, 274, 363
Hpy188III TCNNGA 3 cut(s) 250, 632, 1105
Hpy8I GTNNAC 3 cut(s) 373, 705, 783
HpyAV CCTTC 2 cut(s) 507, 885
HpyCH4III ACNGT 1 cut(s) 1044
HpyCH4IV ACGT 1 cut(s) 987
HpyCH4V TGCA 8 cut(s) 219, 297, 407, 761, 803, 812, 980, 1101
HpyF10VI GCNNNNNNNGC 5 cut(s) 96, 809, 814, 830, 862
HpyF3I CTNAG 5 cut(s) 323, 429, 585, 630, 866
HpySE526I ACGT 1 cut(s) 987
Hsp92II CATG 6 cut(s) 172, 424, 747, 765, 844, 980
HspAI GCGC 4 cut(s) 817, 854, 945, 1083
KspAI GTTAAC 1 cut(s) 373
Kzo9I GATC 4 cut(s) 118, 204, 274, 1183
Lsp1109I GCAGC 2 cut(s) 811, 843
LweI GCATC 3 cut(s) 228, 812, 1110
MaeI CTAG 4 cut(s) 258, 368, 993, 1198
MaeII ACGT 1 cut(s) 987
MaeIII GTNAC 5 cut(s) 105, 128, 553, 1018, 1134
MalI GATC 4 cut(s) 120, 206, 276, 1185
MboI GATC 4 cut(s) 118, 204, 274, 1183
MboII GAAGA 3 cut(s) 74, 278, 408
MfeI CAATTG 1 cut(s) 645
MhlI GDGCHC 1 cut(s) 969
MlsI TGGCCA 1 cut(s) 975
MluCI AATT 9 cut(s) 281, 485, 501, 605, 645, 766, 998, 1173, 1193
MluNI TGGCCA 1 cut(s) 975
MlyI GAGTC 5 cut(s) 29, 66, 540, 561, 749
MmeI TCCRAC 1 cut(s) 535
Mox20I TGGCCA 1 cut(s) 975
MroXI GAANNNNTTC 2 cut(s) 390, 720
MscI TGGCCA 1 cut(s) 975
MseI TTAA 2 cut(s) 279, 372
MslI CAYNNNNRTG 1 cut(s) 173
Msp20I TGGCCA 1 cut(s) 975
MspI CCGG 2 cut(s) 828, 1164
MspR9I CCNGG 3 cut(s) 829, 1164, 1165
MunI CAATTG 1 cut(s) 645
MvnI CGCG 1 cut(s) 945
MwoI GCNNNNNNNGC 5 cut(s) 96, 809, 814, 830, 862
NciI CCSGG 3 cut(s) 829, 1164, 1165
NdeII GATC 4 cut(s) 118, 204, 274, 1183
NlaIII CATG 6 cut(s) 172, 424, 747, 765, 844, 980
NlaIV GGNNCC 1 cut(s) 882
NmeAIII GCCGAG 1 cut(s) 1111
NmuCI GTSAC 5 cut(s) 105, 128, 553, 1018, 1134
NspV TTCGAA 1 cut(s) 394
PceI AGGCCT 1 cut(s) 256
PdmI GAANNNNTTC 2 cut(s) 390, 720
PfeI GAWTC 2 cut(s) 144, 269
PkrI GCNGC 4 cut(s) 602, 735, 826, 858
PleI GAGTC 5 cut(s) 29, 65, 539, 560, 748
PpsI GAGTC 5 cut(s) 29, 65, 539, 560, 748
PspEI GGTNACC 1 cut(s) 128
PspN4I GGNNCC 1 cut(s) 882
PspPI GGNCC 4 cut(s) 508, 880, 938, 1149
PstNI CAGNNNCTG 1 cut(s) 584
RsaI GTAC 4 cut(s) 413, 493, 673, 1059
RsaNI GTAC 4 cut(s) 412, 492, 672, 1058
RseI CAYNNNNRTG 1 cut(s) 173
SaqAI TTAA 2 cut(s) 279, 372
SatI GCNGC 4 cut(s) 601, 734, 825, 857
Sau3AI GATC 4 cut(s) 118, 204, 274, 1183
Sau96I GGNCC 4 cut(s) 508, 880, 938, 1149
ScaI AGTACT 1 cut(s) 413
SchI GAGTC 5 cut(s) 29, 66, 540, 561, 749
ScrFI CCNGG 3 cut(s) 829, 1164, 1165
SduI GDGCHC 1 cut(s) 969
SfaNI GCATC 3 cut(s) 228, 812, 1110
SfcI CTRYAG 1 cut(s) 1123
SfuI TTCGAA 1 cut(s) 394
SinI GGWCC 3 cut(s) 508, 880, 1149
SmaI CCCGGG 1 cut(s) 1165
SmiMI CAYNNNNRTG 1 cut(s) 173
SmlI CTYRAG 1 cut(s) 91
SmoI CTYRAG 1 cut(s) 91
SpeI ACTAGT 1 cut(s) 367
Sse9I AATT 9 cut(s) 281, 485, 501, 605, 645, 766, 998, 1173, 1193
SseBI AGGCCT 1 cut(s) 256
SsiI CCGC 5 cut(s) 333, 544, 598, 601, 734
SspI AATATT 1 cut(s) 889
SspMI CTAG 4 cut(s) 258, 368, 993, 1198
StuI AGGCCT 1 cut(s) 256
StyD4I CCNGG 3 cut(s) 827, 1162, 1163
StyI CCWWGG 2 cut(s) 860, 1187
TaaI ACNGT 1 cut(s) 1044
TaiI ACGT 1 cut(s) 990
TaqI TCGA 3 cut(s) 38, 60, 394
TaqII GACCGA 1 cut(s) 868
TasI AATT 9 cut(s) 281, 485, 501, 605, 645, 766, 998, 1173, 1193
TatI WGTACW 1 cut(s) 411
TauI GCSGC 2 cut(s) 603, 736
TfiI GAWTC 2 cut(s) 144, 269
Tru1I TTAA 2 cut(s) 279, 372
Tru9I TTAA 2 cut(s) 279, 372
TseFI GTSAC 5 cut(s) 105, 128, 553, 1018, 1134
TseI GCWGC 2 cut(s) 824, 856
Tsp45I GTSAC 5 cut(s) 105, 128, 553, 1018, 1134
TspDTI ATGAA 1 cut(s) 279
TspMI CCCGGG 1 cut(s) 1163
VpaK11BI GGWCC 3 cut(s) 508, 880, 1149
XapI RAATTY 3 cut(s) 281, 485, 1193
XmaI CCCGGG 1 cut(s) 1163
XmnI GAANNNNTTC 2 cut(s) 390, 720
XspI CTAG 4 cut(s) 258, 368, 993, 1198
ZrmI AGTACT 1 cut(s) 413
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.