FvH4_2g04810

alpha-L-fucosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0004443
Physical Location & Seq
Reverse (-)
3727402 .. 3733909
6508 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g04810.t1

Sequence Viewer

Length: 2565 bp
ATGCTTTTGGGGGTTCATCTTCTTGTTTCTGTTGGACCCTTTTCATTCTTGTGTGCAATGGAGGATGGTGACTGGGTTCTGGTGCGGCAGCCTGCAGAAAAAGACCTCTGGACTCCTAGTTTAGTGAAGGAGGAGGAGCGTTCTGAGCCTTTGAAGGTTACTTTTGGTGGGCCTGCAAAGTTCTGGACAGATGCAATTCCAATTGGGAATGGGAGGCTTGGAGCTATGGTCTGGGGTGGTGTGGCTTCAGAAACTCTTCAGCTTAATGAGGATACACTCTGGACTGGAACTCCAGGCAACTACACCAACACAAATGCTCCGCAGGCACTAACTGAGGTTAGGCAACTTGTTGATGATGGCAAATATGCTGAAGCTACTGAAGCAGCGGTCAAGTTGACGGGGGATCCTTCTGATGTATACCAACTACTCGGCAATATCAACCTAGAATTTGATGATTTACATCTCAAGTATGCTGAAGAAACTTACAGCAGAGAGCTAGACCTAGATACTGCAACAGCAAGGATAAAATATTCTGTGGGAGAGGTAAAATATACTAGGGAGCATTTTGCTTCAAATCCTGATCAAGTGATTGTGACAAAGATCTCTGCAAGCAAATCAGGGTCCCTATCATTTACAGTTTCGTTAGACAGCAAGTTGCATCATGGTTCTCATGTAAATGGTCAAAATCAAATTATACTTGAAGGAAGTTGTCCTGGTAAAAGAATCGCACCAAAGGCTAATGACAATCCTAAGGGGATTCAGTTTACTGCAGTTCTTGATTTGCAGATTAGTGGTGACAAAGGTGTGATACATTCTCTGGATGACAAGAAAATTAGAGTTGAAGAGGCAGATTGGGCTATTTTGCTTCTGGTAGCCTCATCTTCATTTGAAGATCCATTTACTAAGCCTTCTGATTCTAAGAGAAATCCGACTTCAGAGTCTCTTGCTGCATTGACTTCAATAAAAAGAATTTCATATTCTGACCTCTATGCTCGTCATTTGGATGACTATCAGAATCTCTTTCATCGGGTCTCATTGCAGCTCTCTAAAAGCTCTAAGAGCATATTTGGAAATAAAACCATGGAGATGAAGAAGTGCAACAATATAACCAGTTTAAATTGTAAGGGAAATGACAATGCCTTGGGTTCTACTGCTGACAGGGTGAAAACCTTTAAAATTGATGAAGATCCTTCTTTTGTGGAACTCTTGTTCCAGTATGGTCGATATCTTCTTATTTCCTGTTCACGCCCTGGAACTCAGCCTGCAAACCTGCAGGGTATCTGGAACTACAAAATTGAACCTGCATGGGATGGTGCTCCTCATACGAACATTAATCTTCAGATGAACTACTGGCCTTCCCTTCCTTGTAATCTACGAGAATGCCAGGAACCCTTATTTGATTACACATCTTTATTATCTATTAACGGAAGTAAAACTGCGAAGGTCAACTATGAAGCCAGTGGTTGGGTTGTGCATCAAGTGACTGACATATGGGCGAAAACATCACCGGATCGAGGTCAAGCTGTATGGGCTTTATGGCCAATGGGGGGAGCGTGGATCTGTACTCATCTATGGGAACATTATACTTATACAATGGATAAAGATTTCCTGAAAAATAAGGCATATCCTTTGTTGGAAGGATGTGCATCATTTCTGCTGGACTGGTTGATTGAAGGACGTGGTGGGTATCTTGAAACCAACCCATCAACTTCACCAGAGCACATGTTTATTGCTCCTGATGGTAAGCAGGCCAGTGTGAGCTACTCATCAACCATGGACATCTCAATCATTAAAGAAGTCTTTTCTGCAGTACTTTCTGCTGCTGAGGTTTTAGGAAAAACTCAAAATACTTTTGTTGAAAACGTTCGCAATGCTCAATCAAGGCTTCCACCCACAAAAATTGCTAGAGATGGTTCTATTATGGAATGGGCACAAGATTTTGAGGACCCAGAAGTGCACCATCGACATGTATCCCACCTGTTTGGCCTGTTCCCGGGTCACACAATAACGCTGGGAAAAAATCCGAACCTCTGTAAAGCAGTGGATTATACTCTTTATAAAAGAGGAGAGGAAGGTCCAGGTTGGTCAACTACATGGAAGATTGCATTGTGGGCACGTCTTCACAACAGTGAACATGCATATCGAATGGTCAAGCATTTGATTGACTTGGTGGATCCAGAGCATGAAAGTGATTTTGAAGGAGGACTATACAGTAACTTGTTCACTGCACATCCTCCTTTCCAGATTGATGCCAACTTTGGTTTTTCAGCAGCAGTTGCCGAAATGCTTGTCCAAAGCACAGTCAAGGACTTGTATTTACTTCCTGCTCTTCCCCGGGATAAATGGGCAAATGGTTGTGTCAAAGGCTTGAAGGCACGCGGCGGGGTGACAGTCAACATCTGCTGGAATGAAGGAGATCTTCACGAAGTTGGCCTTTGGTCCAAGGACAACACCACTGTTCAAAAACTACATTATAGAGGAAGTACAGTCACGGCAAACATATCGTCCGGCAGGATATATACTTTCAACAAACAGTTAAAATGTGTGAGGACAGCAAGTCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004553 GO:0004560 GO:0005575 GO:0005576 GO:0015928 GO:0016787 GO:0016798 GO:0047513 GO:0048046
KEGG Pathways
Metabolic & Signaling
ko00511 map00511
Pfam Domains
Protein Families
Glyco_hyd_65N_2

Protein Analysis

855

Amino Acids

95.13

Weight (kDa)

5.92

Isoelectric Point (pI)

30.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hyd_65N_2 PF14498 55 - 297 2.9e-64 Glycosyl hydrolase family 65, N-terminal domain
Glyco_hydro_95_cat PF22124 325 - 763 6.8e-169 Glycosyl hydrolase family 95 catalytic domain
Glyco_hydro_95_C PF21307 765 - 827 5.9e-14 Glycoside hydrolase family 95, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2058
AasI GACNNNNNNGTC 1 cut(s) 937
Acc36I ACCTGC 2 cut(s) 1278, 1309
AccB7I CCANNNNNTGG 1 cut(s) 1464
AccI GTMKAC 1 cut(s) 417
AccII CGCG 1 cut(s) 2379
AciI CCGC 5 cut(s) 85, 320, 386, 2379, 2382
AclI AACGTT 1 cut(s) 1863
AclWI GGATC 8 cut(s) 398, 411, 887, 1181, 1518, 1565, 2168, 2181
AcoI YGGCCR 1 cut(s) 1538
AcsI RAATTY 2 cut(s) 446, 969
AcuI CTGAAG 7 cut(s) 231, 242, 390, 399, 495, 918, 1322
AfaI GTAC 3 cut(s) 1564, 1812, 2485
AfiI CCNNNNNNNGG 4 cut(s) 1464, 1514, 1547, 1993
AflIII ACRYGT 2 cut(s) 1722, 1966
AhdI GACNNNNNGTC 1 cut(s) 2556
AjiI CACGTC 2 cut(s) 1679, 2117
AjnI CCWGG 5 cut(s) 292, 712, 1249, 1383, 2077
AleI CACNNNNGTG 1 cut(s) 2127
AloI GAACNNNNNNTCC 4 cut(s) 1193, 1194, 1225, 1226
AluBI AGCT 8 cut(s) 224, 262, 374, 496, 1042, 1053, 1523, 1761
AluI AGCT 8 cut(s) 224, 262, 374, 496, 1042, 1053, 1523, 1761
Alw21I GWGCWC 3 cut(s) 1318, 1722, 1959
Alw26I GTCTC 2 cut(s) 945, 1036
Alw44I GTGCAC 1 cut(s) 1955
AlwI GGATC 8 cut(s) 398, 411, 887, 1181, 1518, 1565, 2168, 2181
Ama87I CYCGRG 2 cut(s) 1993, 2334
AoxI GGCC 6 cut(s) 170, 1352, 1538, 1749, 1984, 2431
ApaLI GTGCAC 1 cut(s) 1955
ApeKI GCWGC 6 cut(s) 88, 383, 947, 1039, 1820, 2270
ApoI RAATTY 2 cut(s) 446, 969
ArsI GACNNNNNNTTYG 4 cut(s) 2274, 2306, 2489, 2521
AseI ATTAAT 1 cut(s) 1332
Asp700I GAANNNNTTC 2 cut(s) 255, 1863
AspS9I GGNCC 6 cut(s) 35, 170, 621, 1945, 2075, 2439
AsuC2I CCSGG 4 cut(s) 1994, 1995, 2335, 2336
AsuHPI GGTGA 6 cut(s) 80, 806, 1174, 1497, 1704, 2398
AvaI CYCGRG 2 cut(s) 1993, 2334
AvaII GGWCC 5 cut(s) 35, 621, 1945, 2075, 2439
AxyI CCTNAGG 1 cut(s) 750
BaeGI GKGCMC 3 cut(s) 1933, 1959, 2116
BalI TGGCCA 1 cut(s) 1540
BamHI GGATCC 2 cut(s) 403, 2173
BbsI GAAGAC 1 cut(s) 2111
Bbv12I GWGCWC 3 cut(s) 1318, 1722, 1959
BbvCI CCTCAGC 1 cut(s) 1824
BbvI GCAGC 6 cut(s) 100, 395, 934, 1051, 1807, 2282
BccI CCATC 7 cut(s) 59, 350, 1304, 1711, 1733, 1904, 1968
BceAI ACGGC 1 cut(s) 2508
BcgI CGANNNNNNTGC 2 cut(s) 2484, 2518
BciT130I CCWGG 5 cut(s) 294, 714, 1251, 1385, 2079
BciVI GTATCC 2 cut(s) 265, 1981
BclI TGATCA 1 cut(s) 580
BcnI CCSGG 4 cut(s) 1994, 1995, 2335, 2336
BcoDI GTCTC 2 cut(s) 945, 1036
BfaI CTAG 6 cut(s) 117, 443, 497, 503, 555, 1905
BfmI CTRYAG 4 cut(s) 93, 768, 1271, 1806
BfuAI ACCTGC 2 cut(s) 1278, 1309
BfuI GTATCC 2 cut(s) 265, 1981
BglII AGATCT 2 cut(s) 600, 2416
BisI GCNGC 8 cut(s) 86, 89, 384, 948, 1040, 1821, 2271, 2380
BlsI GCNGC 8 cut(s) 87, 90, 385, 949, 1041, 1822, 2272, 2381
BmcAI AGTACT 1 cut(s) 1812
Bme1390I CCNGG 9 cut(s) 294, 714, 1251, 1385, 1994, 1995, 2079, 2335, 2336
Bme18I GGWCC 5 cut(s) 35, 621, 1945, 2075, 2439
BmeRI GACNNNNNGTC 1 cut(s) 2556
BmeT110I CYCGRG 2 cut(s) 1993, 2334
BmgBI CACGTC 2 cut(s) 1679, 2117
BmgT120I GGNCC 6 cut(s) 35, 170, 621, 1945, 2075, 2439
BmiI GGNNCC 7 cut(s) 37, 405, 622, 623, 1389, 1947, 2175
BmrFI CCNGG 9 cut(s) 294, 714, 1251, 1385, 1994, 1995, 2079, 2335, 2336
BmrI ACTGGG 1 cut(s) 82
BmsI GCATC 5 cut(s) 181, 667, 1483, 1655, 2239
BmuI ACTGGG 1 cut(s) 82
BpiI GAAGAC 1 cut(s) 2111
BpmI CTGGAG 1 cut(s) 276
Bpu10I CCTNAGC 1 cut(s) 1824
BpuEI CTTGAG 1 cut(s) 449
BpuMI CCSGG 4 cut(s) 1994, 1995, 2335, 2336
BsaBI GATNNNNATC 4 cut(s) 459, 1008, 1185, 1645
BsaI GGTCTC 1 cut(s) 1036
BsaJI CCNNGG 8 cut(s) 1080, 1140, 1249, 1773, 1993, 2333, 2334, 2442
BsaWI WCCGGW 1 cut(s) 1507
BsaXI ACNNNNNCTCC 6 cut(s) 213, 243, 274, 301, 304, 331
Bsc4I CCNNNNNNNGG 4 cut(s) 1464, 1514, 1547, 1993
Bse1I ACTGG 8 cut(s) 77, 289, 1110, 1213, 1355, 1458, 1667, 1752
Bse21I CCTNAGG 1 cut(s) 750
Bse3DI GCAATG 3 cut(s) 63, 1034, 1876
Bse8I GATNNNNATC 4 cut(s) 459, 1008, 1185, 1645
BseBI CCWGG 5 cut(s) 294, 714, 1251, 1385, 2079
BseDI CCNNGG 8 cut(s) 1080, 1140, 1249, 1773, 1993, 2333, 2334, 2442
BseGI GGATG 6 cut(s) 70, 826, 1009, 1315, 1646, 2230
BseJI GATNNNNATC 4 cut(s) 459, 1008, 1185, 1645
BseLI CCNNNNNNNGG 4 cut(s) 1464, 1514, 1547, 1993
BseMI GCAATG 3 cut(s) 63, 1034, 1876
BseMII CTCAG 4 cut(s) 135, 324, 1271, 1815
BseNI ACTGG 8 cut(s) 77, 289, 1110, 1213, 1355, 1458, 1667, 1752
BseRI GAGGAG 4 cut(s) 146, 149, 1308, 2079
BseSI GKGCMC 3 cut(s) 1933, 1959, 2116
BseXI GCAGC 6 cut(s) 100, 395, 934, 1051, 1807, 2282
BseYI CCCAGC 1 cut(s) 2011
BsgI GTGCAG 1 cut(s) 2211
Bsh1236I CGCG 1 cut(s) 2379
BshFI GGCC 6 cut(s) 172, 1354, 1540, 1751, 1986, 2433
BsiHKAI GWGCWC 3 cut(s) 1318, 1722, 1959
BsiHKCI CYCGRG 2 cut(s) 1993, 2334
BsiSI CCGG 4 cut(s) 1508, 1994, 2335, 2508
BslFI GGGAC 1 cut(s) 607
BslI CCNNNNNNNGG 4 cut(s) 1464, 1514, 1547, 1993
BsmAI GTCTC 2 cut(s) 945, 1036
BsmFI GGGAC 1 cut(s) 607
BsmI GAATGC 1 cut(s) 1385
BsnI GGCC 6 cut(s) 172, 1354, 1540, 1751, 1986, 2433
Bso31I GGTCTC 1 cut(s) 1036
BsoBI CYCGRG 2 cut(s) 1993, 2334
Bsp1286I GDGCHC 5 cut(s) 1318, 1722, 1933, 1959, 2116
Bsp143I GATC 9 cut(s) 403, 580, 600, 892, 1186, 1510, 1557, 2173, 2416
Bsp19I CCATGG 2 cut(s) 1080, 1773
BspACI CCGC 5 cut(s) 85, 320, 386, 2379, 2382
BspANI GGCC 6 cut(s) 172, 1354, 1540, 1751, 1986, 2433
BspCNI CTCAG 4 cut(s) 136, 325, 1270, 1816
BspFNI CGCG 1 cut(s) 2379
BspLI GGNNCC 7 cut(s) 37, 405, 622, 623, 1389, 1947, 2175
BspMAI CTGCAG 4 cut(s) 97, 772, 1275, 1810
BspMI ACCTGC 2 cut(s) 1278, 1309
BspPI GGATC 8 cut(s) 398, 411, 887, 1181, 1518, 1565, 2168, 2181
BspQI GCTCTTC 1 cut(s) 2334
BspTNI GGTCTC 1 cut(s) 1036
BsrDI GCAATG 3 cut(s) 63, 1034, 1876
BsrI ACTGG 8 cut(s) 77, 289, 1110, 1213, 1355, 1458, 1667, 1752
BssECI CCNNGG 8 cut(s) 1080, 1140, 1249, 1773, 1993, 2333, 2334, 2442
BssMI GATC 9 cut(s) 403, 580, 600, 892, 1186, 1510, 1557, 2173, 2416
BssNAI GTATAC 1 cut(s) 418
BssT1I CCWWGG 4 cut(s) 1080, 1140, 1773, 2442
Bst1107I GTATAC 1 cut(s) 418
Bst2UI CCWGG 5 cut(s) 294, 714, 1251, 1385, 2079
Bst4CI ACNGT 8 cut(s) 637, 2129, 2213, 2302, 2392, 2458, 2488, 2535
Bst6I CTCTTC 3 cut(s) 261, 837, 2334
BstAPI GCANNNNNTGC 1 cut(s) 2276
BstC8I GCNNGC 7 cut(s) 93, 174, 324, 610, 1263, 1749, 2377
BstDEI CTNAG 8 cut(s) 144, 333, 750, 903, 918, 1056, 1257, 1824
BstDSI CCRYGG 2 cut(s) 1080, 1773
BstF5I GGATG 6 cut(s) 70, 826, 1009, 1315, 1646, 2230
BstFNI CGCG 1 cut(s) 2379
BstKTI GATC 9 cut(s) 406, 583, 603, 895, 1189, 1513, 1560, 2176, 2419
BstMAI GTCTC 2 cut(s) 945, 1036
BstMBI GATC 9 cut(s) 403, 580, 600, 892, 1186, 1510, 1557, 2173, 2416
BstMWI GCNNNNNNNGC 9 cut(s) 145, 323, 380, 734, 854, 1059, 1529, 2111, 2276
BstNI CCWGG 5 cut(s) 294, 714, 1251, 1385, 2079
BstNSI RCATGY 3 cut(s) 1726, 1970, 2138
BstSCI CCNGG 9 cut(s) 292, 712, 1249, 1383, 1992, 1993, 2077, 2333, 2334
BstSFI CTRYAG 4 cut(s) 93, 768, 1271, 1806
BstSLI GKGCMC 3 cut(s) 1933, 1959, 2116
BstUI CGCG 1 cut(s) 2379
BstV1I GCAGC 6 cut(s) 100, 395, 934, 1051, 1807, 2282
BstV2I GAAGAC 1 cut(s) 2111
BstX2I RGATCY 7 cut(s) 403, 600, 892, 1186, 1557, 2173, 2416
BstXI CCANNNNNNTGG 1 cut(s) 1982
BstYI RGATCY 7 cut(s) 403, 600, 892, 1186, 1557, 2173, 2416
BstZ17I GTATAC 1 cut(s) 418
Bsu36I CCTNAGG 1 cut(s) 750
BsuI GTATCC 2 cut(s) 265, 1981
BsuRI GGCC 6 cut(s) 172, 1354, 1540, 1751, 1986, 2433
BtgI CCRYGG 2 cut(s) 1080, 1773
BtrI CACGTC 2 cut(s) 1679, 2117
BtsCI GGATG 6 cut(s) 70, 826, 1009, 1315, 1646, 2230
BtsI GCAGTG 2 cut(s) 2046, 2223
BtsIMutI CAGTG 6 cut(s) 1465, 1759, 2046, 2134, 2223, 2454
BveI ACCTGC 2 cut(s) 1278, 1309
Cac8I GCNNGC 7 cut(s) 93, 174, 324, 610, 1263, 1749, 2377
Cfr13I GGNCC 6 cut(s) 35, 170, 621, 1945, 2075, 2439
Cfr9I CCCGGG 2 cut(s) 1993, 2334
Csp6I GTAC 3 cut(s) 1563, 1811, 2484
CspCI CAANNNNNGTGG 2 cut(s) 1882, 1917
CviQI GTAC 3 cut(s) 1563, 1811, 2484
DdeI CTNAG 8 cut(s) 144, 333, 750, 903, 918, 1056, 1257, 1824
DpnI GATC 9 cut(s) 405, 582, 602, 894, 1188, 1512, 1559, 2175, 2418
DpnII GATC 9 cut(s) 403, 580, 600, 892, 1186, 1510, 1557, 2173, 2416
DraI TTTAAA 2 cut(s) 1116, 1174
DrdI GACNNNNNNGTC 1 cut(s) 937
DriI GACNNNNNGTC 1 cut(s) 2556
DseDI GACNNNNNNGTC 1 cut(s) 937
EaeI YGGCCR 1 cut(s) 1538
Eam1104I CTCTTC 3 cut(s) 261, 837, 2334
Eam1105I GACNNNNNGTC 1 cut(s) 2556
EarI CTCTTC 3 cut(s) 261, 837, 2334
Eco130I CCWWGG 4 cut(s) 1080, 1140, 1773, 2442
Eco31I GGTCTC 1 cut(s) 1036
Eco32I GATATC 1 cut(s) 1226
Eco47I GGWCC 5 cut(s) 35, 621, 1945, 2075, 2439
Eco57I CTGAAG 7 cut(s) 231, 242, 390, 399, 495, 918, 1322
Eco81I CCTNAGG 1 cut(s) 750
Eco88I CYCGRG 2 cut(s) 1993, 2334
EcoO109I RGGNCCY 2 cut(s) 621, 1945
EcoRII CCWGG 5 cut(s) 292, 712, 1249, 1383, 2077
EcoRV GATATC 1 cut(s) 1226
EcoT14I CCWWGG 4 cut(s) 1080, 1140, 1773, 2442
EcoT22I ATGCAT 1 cut(s) 2140
ErhI CCWWGG 4 cut(s) 1080, 1140, 1773, 2442
FalI AAGNNNNNCTT 4 cut(s) 2403, 2435, 2418, 2450
FaqI GGGAC 1 cut(s) 607
FauI CCCGC 1 cut(s) 2375
FauNDI CATATG 1 cut(s) 1490
FbaI TGATCA 1 cut(s) 580
FblI GTMKAC 1 cut(s) 417
Fnu4HI GCNGC 8 cut(s) 86, 89, 384, 948, 1040, 1821, 2271, 2380
FokI GGATG 6 cut(s) 77, 833, 1016, 1322, 1653, 2217
Fsp4HI GCNGC 8 cut(s) 86, 89, 384, 948, 1040, 1821, 2271, 2380
FspBI CTAG 6 cut(s) 117, 443, 497, 503, 555, 1905
GluI GCNGC 8 cut(s) 86, 89, 384, 948, 1040, 1821, 2271, 2380
GsaI CCCAGC 1 cut(s) 2015
GsuI CTGGAG 1 cut(s) 276
HaeIII GGCC 6 cut(s) 172, 1354, 1540, 1751, 1986, 2433
HapII CCGG 4 cut(s) 1508, 1994, 2335, 2508
HincII GTYRAC 4 cut(s) 396, 1447, 2088, 2395
HindII GTYRAC 4 cut(s) 396, 1447, 2088, 2395
HinfI GANTC 6 cut(s) 112, 723, 757, 914, 938, 1015
HpaII CCGG 4 cut(s) 1508, 1994, 2335, 2508
HphI GGTGA 6 cut(s) 80, 806, 1174, 1497, 1704, 2398
HpyCH4III ACNGT 8 cut(s) 637, 2129, 2213, 2302, 2392, 2458, 2488, 2535
HpyCH4IV ACGT 3 cut(s) 1678, 1863, 2116
HpyF10VI GCNNNNNNNGC 9 cut(s) 145, 323, 380, 734, 854, 1059, 1529, 2111, 2276
HpyF3I CTNAG 8 cut(s) 144, 333, 750, 903, 918, 1056, 1257, 1824
HpySE526I ACGT 3 cut(s) 1678, 1863, 2116
KflI GGGWCCC 1 cut(s) 621
Ksp22I TGATCA 1 cut(s) 580
Kzo9I GATC 9 cut(s) 403, 580, 600, 892, 1186, 1510, 1557, 2173, 2416
LguI GCTCTTC 1 cut(s) 2334
LmnI GCTCC 7 cut(s) 136, 221, 322, 559, 1321, 1550, 1738
Lsp1109I GCAGC 6 cut(s) 100, 395, 934, 1051, 1807, 2282
LweI GCATC 5 cut(s) 181, 667, 1483, 1655, 2239
MaeI CTAG 6 cut(s) 117, 443, 497, 503, 555, 1905
MaeII ACGT 3 cut(s) 1678, 1863, 2116
MaeIII GTNAC 9 cut(s) 68, 157, 592, 794, 1480, 1997, 2213, 2386, 2488
MalI GATC 9 cut(s) 405, 582, 602, 894, 1188, 1512, 1559, 2175, 2418
MboI GATC 9 cut(s) 403, 580, 600, 892, 1186, 1510, 1557, 2173, 2416
MfeI CAATTG 1 cut(s) 201
MflI RGATCY 7 cut(s) 403, 600, 892, 1186, 1557, 2173, 2416
MhlI GDGCHC 5 cut(s) 1318, 1722, 1933, 1959, 2116
MlsI TGGCCA 1 cut(s) 1540
MluNI TGGCCA 1 cut(s) 1540
MlyI GAGTC 2 cut(s) 106, 947
MmeI TCCRAC 3 cut(s) 13, 953, 1614
Mox20I TGGCCA 1 cut(s) 1540
Mph1103I ATGCAT 1 cut(s) 2140
MroXI GAANNNNTTC 2 cut(s) 255, 1863
MscI TGGCCA 1 cut(s) 1540
MseI TTAA 7 cut(s) 264, 1115, 1173, 1332, 1422, 1791, 2537
MslI CAYNNNNRTG 7 cut(s) 49, 675, 1002, 1085, 1965, 2127, 2187
Msp20I TGGCCA 1 cut(s) 1540
MspA1I CMGCKG 1 cut(s) 386
MspI CCGG 4 cut(s) 1508, 1994, 2335, 2508
MspR9I CCNGG 9 cut(s) 294, 714, 1251, 1385, 1994, 1995, 2079, 2335, 2336
MunI CAATTG 1 cut(s) 201
Mva1269I GAATGC 1 cut(s) 1385
MvaI CCWGG 5 cut(s) 294, 714, 1251, 1385, 2079
MvnI CGCG 1 cut(s) 2379
MwoI GCNNNNNNNGC 9 cut(s) 145, 323, 380, 734, 854, 1059, 1529, 2111, 2276
NciI CCSGG 4 cut(s) 1994, 1995, 2335, 2336
NcoI CCATGG 2 cut(s) 1080, 1773
NdeI CATATG 1 cut(s) 1490
NdeII GATC 9 cut(s) 403, 580, 600, 892, 1186, 1510, 1557, 2173, 2416
NlaIV GGNNCC 7 cut(s) 37, 405, 622, 623, 1389, 1947, 2175
NmeAIII GCCGAG 1 cut(s) 408
NmuCI GTSAC 7 cut(s) 68, 592, 794, 1480, 1997, 2386, 2488
NsiI ATGCAT 1 cut(s) 2140
NspI RCATGY 3 cut(s) 1726, 1970, 2138
OliI CACNNNNGTG 1 cut(s) 2127
PciI ACATGT 2 cut(s) 1722, 1966
PciSI GCTCTTC 1 cut(s) 2334
PctI GAATGC 1 cut(s) 1385
PdmI GAANNNNTTC 2 cut(s) 255, 1863
PfeI GAWTC 4 cut(s) 723, 757, 914, 1015
PflMI CCANNNNNTGG 1 cut(s) 1464
PkrI GCNGC 8 cut(s) 87, 90, 385, 949, 1041, 1822, 2272, 2381
PleI GAGTC 2 cut(s) 106, 946
PpsI GAGTC 2 cut(s) 106, 946
PpuMI RGGWCCY 2 cut(s) 621, 1945
PscI ACATGT 2 cut(s) 1722, 1966
PshBI ATTAAT 1 cut(s) 1332
PsiI TTATAA 1 cut(s) 2058
Psp1406I AACGTT 1 cut(s) 1863
Psp5II RGGWCCY 2 cut(s) 621, 1945
Psp6I CCWGG 5 cut(s) 292, 712, 1249, 1383, 2077
PspFI CCCAGC 1 cut(s) 2011
PspGI CCWGG 5 cut(s) 292, 712, 1249, 1383, 2077
PspN4I GGNNCC 7 cut(s) 37, 405, 622, 623, 1389, 1947, 2175
PspPI GGNCC 6 cut(s) 35, 170, 621, 1945, 2075, 2439
PspPPI RGGWCCY 2 cut(s) 621, 1945
PstI CTGCAG 4 cut(s) 97, 772, 1275, 1810
PsuI RGATCY 7 cut(s) 403, 600, 892, 1186, 1557, 2173, 2416
RsaI GTAC 3 cut(s) 1564, 1812, 2485
RsaNI GTAC 3 cut(s) 1563, 1811, 2484
RseI CAYNNNNRTG 7 cut(s) 49, 675, 1002, 1085, 1965, 2127, 2187
SapI GCTCTTC 1 cut(s) 2334
SaqAI TTAA 7 cut(s) 264, 1115, 1173, 1332, 1422, 1791, 2537
SatI GCNGC 8 cut(s) 86, 89, 384, 948, 1040, 1821, 2271, 2380
Sau3AI GATC 9 cut(s) 403, 580, 600, 892, 1186, 1510, 1557, 2173, 2416
Sau96I GGNCC 6 cut(s) 35, 170, 621, 1945, 2075, 2439
SbfI CCTGCAGG 1 cut(s) 1275
ScaI AGTACT 1 cut(s) 1812
SchI GAGTC 2 cut(s) 106, 947
ScrFI CCNGG 9 cut(s) 294, 714, 1251, 1385, 1994, 1995, 2079, 2335, 2336
SdaI CCTGCAGG 1 cut(s) 1275
SduI GDGCHC 5 cut(s) 1318, 1722, 1933, 1959, 2116
SfaNI GCATC 5 cut(s) 181, 667, 1483, 1655, 2239
SfcI CTRYAG 4 cut(s) 93, 768, 1271, 1806
SinI GGWCC 5 cut(s) 35, 621, 1945, 2075, 2439
SmaI CCCGGG 2 cut(s) 1995, 2336
SmiMI CAYNNNNRTG 7 cut(s) 49, 675, 1002, 1085, 1965, 2127, 2187
SmlI CTYRAG 1 cut(s) 464
SmoI CTYRAG 1 cut(s) 464
Sse8387I CCTGCAGG 1 cut(s) 1275
SsiI CCGC 5 cut(s) 85, 320, 386, 2379, 2382
SspI AATATT 1 cut(s) 530
SspMI CTAG 6 cut(s) 117, 443, 497, 503, 555, 1905
StyD4I CCNGG 9 cut(s) 292, 712, 1249, 1383, 1992, 1993, 2077, 2333, 2334
StyI CCWWGG 4 cut(s) 1080, 1140, 1773, 2442
TaaI ACNGT 8 cut(s) 637, 2129, 2213, 2302, 2392, 2458, 2488, 2535
TaiI ACGT 3 cut(s) 1681, 1866, 2119
TaqI TCGA 4 cut(s) 1222, 1513, 1963, 2143
TatI WGTACW 3 cut(s) 1562, 1810, 2483
TauI GCSGC 2 cut(s) 88, 2382
TfiI GAWTC 4 cut(s) 723, 757, 914, 1015
Tru1I TTAA 7 cut(s) 264, 1115, 1173, 1332, 1422, 1791, 2537
Tru9I TTAA 7 cut(s) 264, 1115, 1173, 1332, 1422, 1791, 2537
TscAI CASTG 6 cut(s) 1465, 1759, 2046, 2134, 2230, 2461
TseFI GTSAC 7 cut(s) 68, 592, 794, 1480, 1997, 2386, 2488
TseI GCWGC 6 cut(s) 88, 383, 947, 1039, 1820, 2270
Tsp45I GTSAC 7 cut(s) 68, 592, 794, 1480, 1997, 2386, 2488
TspGWI ACGGA 1 cut(s) 1440
TspMI CCCGGG 2 cut(s) 1993, 2334
TspRI CASTG 6 cut(s) 1465, 1759, 2046, 2134, 2230, 2461
Van91I CCANNNNNTGG 1 cut(s) 1464
VneI GTGCAC 1 cut(s) 1955
VpaK11BI GGWCC 5 cut(s) 35, 621, 1945, 2075, 2439
VspI ATTAAT 1 cut(s) 1332
XapI RAATTY 2 cut(s) 446, 969
XceI RCATGY 3 cut(s) 1726, 1970, 2138
XmaI CCCGGG 2 cut(s) 1993, 2334
XmiI GTMKAC 1 cut(s) 417
XmnI GAANNNNTTC 2 cut(s) 255, 1863
XspI CTAG 6 cut(s) 117, 443, 497, 503, 555, 1905
ZrmI AGTACT 1 cut(s) 1812
Zsp2I ATGCAT 1 cut(s) 2140
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.