RchiOBHm_Chr6g0254581

alpha-L-fucosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0004443
Physical Location & Seq
Reverse (-)
9572528 .. 9575476
2949 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ22837

Sequence Viewer

Length: 942 bp
ATGGAGGATGGTGACTGGGTTCTGGTGCGGCCGCCTGCAGAGAAGGACCTCTGGACCCCAAGTTTAGTGAAGGAGGATGAGAGTTCTAAGCCTTTGAAGGTTACTTTTGGTGGGCCTGCGAAGTTCTGGACTGATGCAATTCCAATTGGGAATGGGAGGCTTGGAGCTATGGTCTGGGGTGGTGTGCCTTCAGAAACTCTTCAGCTCAATGAGGACACACTCTGGGCTGGAACTCCGGGCAACTACACCAACCCAAATGCTCCACAGGCACTAACAGAGGTTAGGAAACTTGTTGATGATGGTAAATATGTTGAAGCTACTGAAGCTGCGGTCAAGTTGTCCGGAGATCCTTCTGATGTATACCAACTACTCGGCGACATCAACCTAGAATTTGAGGATTCGCATCTCAAGTATGCTGAAGAAACTTACAGCAGAGAGCTAGACCTAGATACTGCAACAGCAAGGATAAAATATTCTGTGGGTGATGTAGAATATACTAGGGAGCATTTTGCTTCGAATCCCGATCAAGTGATTGTGACAAAGATCTCTGCAAGTAAATCAGGGTCCCTATCATTCACAGTTTCTCTAGACAGCAAGATGCATCATGGTTCACATTTAAATGGTAAAAACCAAATTATACTTGAAGGAAGTTGTCCTGGTAGAAGAATTGCACCAAAGTATAATGACAATCCAGAGGGGATTCAGTTTACTGCAGTTCTTGATTTGCAGATTAGTGGTGACAAAGGTGTGATACATGCTTTGGATGACAAGAAATTTAGAGTTGAAGAGGCAGATTCGGCTGTTTTGCTTCTGGTGGCCTCATCTTCATTTAAGGATGCATTTACTAAGCCTTCTGATTCTAAGAGAAATCCAACTTCAGAGTCTCTCACTGCATTGAATTCAATAAGAAATTTCTCTCTAAGAGCTCTAAGAGCATATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004553 GO:0004560 GO:0005575 GO:0005576 GO:0015928 GO:0016787 GO:0016798 GO:0047513 GO:0048046
KEGG Pathways
Metabolic & Signaling
ko00511 map00511
Pfam Domains
Protein Families
Glyco_hyd_65N_2

Protein Analysis

313

Amino Acids

34.29

Weight (kDa)

5.04

Isoelectric Point (pI)

31.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hyd_65N_2 PF14498 36 - 279 1.7e-64 Glycosyl hydrolase family 65, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 360
AccIII TCCGGA 1 cut(s) 341
AciI CCGC 3 cut(s) 28, 32, 329
AclWI GGATC 1 cut(s) 341
AcoI YGGCCR 1 cut(s) 29
AcsI RAATTY 4 cut(s) 389, 773, 898, 910
AcuI CTGAAG 5 cut(s) 174, 185, 342, 438, 861
AgsI TTSAA 6 cut(s) 97, 314, 644, 785, 898, 903
AjnI CCWGG 1 cut(s) 655
AluBI AGCT 6 cut(s) 167, 205, 317, 326, 439, 926
AluI AGCT 6 cut(s) 167, 205, 317, 326, 439, 926
Alw21I GWGCWC 1 cut(s) 928
Alw26I GTCTC 1 cut(s) 888
AlwI GGATC 1 cut(s) 341
Aor13HI TCCGGA 1 cut(s) 341
AoxI GGCC 3 cut(s) 29, 113, 816
ApeKI GCWGC 1 cut(s) 326
ApoI RAATTY 4 cut(s) 389, 773, 898, 910
Asp700I GAANNNNTTC 1 cut(s) 198
AspS9I GGNCC 4 cut(s) 46, 54, 113, 564
AsuC2I CCSGG 1 cut(s) 237
AsuHPI GGTGA 3 cut(s) 23, 494, 749
AsuII TTCGAA 1 cut(s) 515
AvaII GGWCC 3 cut(s) 46, 54, 564
BanII GRGCYC 1 cut(s) 928
Bbv12I GWGCWC 1 cut(s) 928
BbvI GCAGC 1 cut(s) 313
BccI CCATC 2 cut(s) 2, 293
BciT130I CCWGG 1 cut(s) 657
BcnI CCSGG 1 cut(s) 237
BcoDI GTCTC 1 cut(s) 888
BfaI CTAG 5 cut(s) 386, 440, 446, 498, 587
BfmI CTRYAG 2 cut(s) 36, 711
BglII AGATCT 1 cut(s) 543
BisI GCNGC 3 cut(s) 29, 32, 327
BlsI GCNGC 3 cut(s) 30, 33, 328
Bme1390I CCNGG 2 cut(s) 237, 657
Bme18I GGWCC 3 cut(s) 46, 54, 564
BmgT120I GGNCC 4 cut(s) 46, 54, 113, 564
BmiI GGNNCC 3 cut(s) 56, 565, 566
BmrFI CCNGG 2 cut(s) 237, 657
BmrI ACTGGG 1 cut(s) 25
BmsI GCATC 5 cut(s) 124, 412, 588, 610, 826
BmuI ACTGGG 1 cut(s) 25
Bpu14I TTCGAA 1 cut(s) 515
BpuEI CTTGAG 1 cut(s) 392
BpuMI CCSGG 1 cut(s) 237
BsaBI GATNNNNATC 1 cut(s) 402
BsaWI WCCGGW 1 cut(s) 341
BsaXI ACNNNNNCTCC 2 cut(s) 156, 186
Bse1I ACTGG 1 cut(s) 20
Bse8I GATNNNNATC 1 cut(s) 402
BseAI TCCGGA 1 cut(s) 341
BseBI CCWGG 1 cut(s) 657
BseGI GGATG 4 cut(s) 13, 82, 769, 841
BseJI GATNNNNATC 1 cut(s) 402
BseNI ACTGG 1 cut(s) 20
BseX3I CGGCCG 1 cut(s) 29
BseXI GCAGC 1 cut(s) 313
Bsh1285I CGRYCG 1 cut(s) 32
BshFI GGCC 3 cut(s) 31, 115, 818
BsiEI CGRYCG 1 cut(s) 32
BsiHKAI GWGCWC 1 cut(s) 928
BsiSI CCGG 2 cut(s) 236, 342
BslFI GGGAC 1 cut(s) 550
BsmAI GTCTC 1 cut(s) 888
BsmFI GGGAC 1 cut(s) 550
BsnI GGCC 3 cut(s) 31, 115, 818
Bsp119I TTCGAA 1 cut(s) 515
Bsp1286I GDGCHC 1 cut(s) 928
Bsp13I TCCGGA 1 cut(s) 341
Bsp143I GATC 3 cut(s) 346, 523, 543
BspACI CCGC 3 cut(s) 28, 32, 329
BspANI GGCC 3 cut(s) 31, 115, 818
BspEI TCCGGA 1 cut(s) 341
BspLI GGNNCC 3 cut(s) 56, 565, 566
BspMAI CTGCAG 2 cut(s) 40, 715
BspPI GGATC 1 cut(s) 341
BspT104I TTCGAA 1 cut(s) 515
BsrI ACTGG 1 cut(s) 20
BssMI GATC 3 cut(s) 346, 523, 543
BssNAI GTATAC 1 cut(s) 361
Bst1107I GTATAC 1 cut(s) 361
Bst2UI CCWGG 1 cut(s) 657
Bst4CI ACNGT 1 cut(s) 580
Bst6I CTCTTC 2 cut(s) 204, 780
BstBI TTCGAA 1 cut(s) 515
BstC8I GCNNGC 2 cut(s) 36, 117
BstDEI CTNAG 5 cut(s) 87, 846, 861, 920, 929
BstF5I GGATG 4 cut(s) 13, 82, 769, 841
BstKTI GATC 3 cut(s) 349, 526, 546
BstMAI GTCTC 1 cut(s) 888
BstMBI GATC 3 cut(s) 346, 523, 543
BstMCI CGRYCG 1 cut(s) 32
BstMWI GCNNNNNNNGC 4 cut(s) 266, 323, 797, 932
BstNI CCWGG 1 cut(s) 657
BstNSI RCATGY 1 cut(s) 758
BstSCI CCNGG 2 cut(s) 235, 655
BstSFI CTRYAG 2 cut(s) 36, 711
BstV1I GCAGC 1 cut(s) 313
BstX2I RGATCY 2 cut(s) 346, 543
BstYI RGATCY 2 cut(s) 346, 543
BstZ17I GTATAC 1 cut(s) 361
BstZI CGGCCG 1 cut(s) 29
BsuRI GGCC 3 cut(s) 31, 115, 818
BtsCI GGATG 4 cut(s) 13, 82, 769, 841
BtsI GCAGTG 1 cut(s) 888
BtsIMutI CAGTG 1 cut(s) 888
Cac8I GCNNGC 2 cut(s) 36, 117
CciNI GCGGCCGC 1 cut(s) 29
Cfr13I GGNCC 4 cut(s) 46, 54, 113, 564
CviAII CATG 2 cut(s) 605, 755
DdeI CTNAG 5 cut(s) 87, 846, 861, 920, 929
DpnI GATC 3 cut(s) 348, 525, 545
DpnII GATC 3 cut(s) 346, 523, 543
DraI TTTAAA 1 cut(s) 618
EaeI YGGCCR 1 cut(s) 29
EagI CGGCCG 1 cut(s) 29
Eam1104I CTCTTC 2 cut(s) 204, 780
EarI CTCTTC 2 cut(s) 204, 780
Ecl136II GAGCTC 1 cut(s) 926
EclXI CGGCCG 1 cut(s) 29
Eco24I GRGCYC 1 cut(s) 928
Eco47I GGWCC 3 cut(s) 46, 54, 564
Eco52I CGGCCG 1 cut(s) 29
Eco53kI GAGCTC 1 cut(s) 926
Eco57I CTGAAG 5 cut(s) 174, 185, 342, 438, 861
EcoICRI GAGCTC 1 cut(s) 926
EcoO109I RGGNCCY 2 cut(s) 46, 564
EcoRI GAATTC 1 cut(s) 898
EcoRII CCWGG 1 cut(s) 655
EcoT22I ATGCAT 2 cut(s) 603, 841
EcoT38I GRGCYC 1 cut(s) 928
FaeI CATG 2 cut(s) 608, 758
FaqI GGGAC 1 cut(s) 550
FatI CATG 2 cut(s) 604, 754
FblI GTMKAC 1 cut(s) 360
Fnu4HI GCNGC 3 cut(s) 29, 32, 327
FokI GGATG 4 cut(s) 20, 89, 776, 848
FriOI GRGCYC 1 cut(s) 928
Fsp4HI GCNGC 3 cut(s) 29, 32, 327
FspBI CTAG 5 cut(s) 386, 440, 446, 498, 587
GluI GCNGC 3 cut(s) 29, 32, 327
HaeIII GGCC 3 cut(s) 31, 115, 818
HapII CCGG 2 cut(s) 236, 342
Hin1II CATG 2 cut(s) 608, 758
HinfI GANTC 6 cut(s) 398, 517, 700, 794, 857, 881
HpaII CCGG 2 cut(s) 236, 342
HphI GGTGA 3 cut(s) 23, 494, 749
Hpy166II GTNNAC 3 cut(s) 361, 611, 708
Hpy188I TCNGA 4 cut(s) 193, 355, 856, 880
Hpy188III TCNNGA 7 cut(s) 52, 127, 342, 521, 587, 692, 719
Hpy8I GTNNAC 3 cut(s) 361, 611, 708
HpyAV CCTTC 7 cut(s) 37, 64, 91, 198, 360, 638, 861
HpyCH4III ACNGT 1 cut(s) 580
HpyF10VI GCNNNNNNNGC 4 cut(s) 266, 323, 797, 932
HpyF3I CTNAG 5 cut(s) 87, 846, 861, 920, 929
Hsp92II CATG 2 cut(s) 608, 758
KflI GGGWCCC 1 cut(s) 564
Kpn2I TCCGGA 1 cut(s) 341
Kzo9I GATC 3 cut(s) 346, 523, 543
LmnI GCTCC 3 cut(s) 164, 265, 502
Lsp1109I GCAGC 1 cut(s) 313
LweI GCATC 5 cut(s) 124, 412, 588, 610, 826
MaeI CTAG 5 cut(s) 386, 440, 446, 498, 587
MaeIII GTNAC 4 cut(s) 11, 100, 535, 737
MalI GATC 3 cut(s) 348, 525, 545
MboI GATC 3 cut(s) 346, 523, 543
MboII GAAGA 5 cut(s) 191, 431, 675, 797, 816
MfeI CAATTG 1 cut(s) 144
MflI RGATCY 2 cut(s) 346, 543
MhlI GDGCHC 1 cut(s) 928
MluCI AATT 8 cut(s) 138, 144, 389, 633, 666, 773, 898, 910
MlyI GAGTC 1 cut(s) 890
MmeI TCCRAC 1 cut(s) 896
MnlI CCTC 9 cut(s) 59, 67, 150, 205, 271, 388, 688, 781, 829
Mph1103I ATGCAT 2 cut(s) 603, 841
MroI TCCGGA 1 cut(s) 341
MroXI GAANNNNTTC 1 cut(s) 198
MseI TTAA 2 cut(s) 617, 831
MslI CAYNNNNRTG 1 cut(s) 618
MspI CCGG 2 cut(s) 236, 342
MspR9I CCNGG 2 cut(s) 237, 657
MunI CAATTG 1 cut(s) 144
MvaI CCWGG 1 cut(s) 657
MwoI GCNNNNNNNGC 4 cut(s) 266, 323, 797, 932
NciI CCSGG 1 cut(s) 237
NdeII GATC 3 cut(s) 346, 523, 543
NlaIII CATG 2 cut(s) 608, 758
NlaIV GGNNCC 3 cut(s) 56, 565, 566
NmeAIII GCCGAG 1 cut(s) 351
NmuCI GTSAC 3 cut(s) 11, 535, 737
NotI GCGGCCGC 1 cut(s) 29
NsiI ATGCAT 2 cut(s) 603, 841
NspI RCATGY 1 cut(s) 758
NspV TTCGAA 1 cut(s) 515
PdmI GAANNNNTTC 1 cut(s) 198
PfeI GAWTC 5 cut(s) 398, 517, 700, 794, 857
PkrI GCNGC 3 cut(s) 30, 33, 328
PleI GAGTC 1 cut(s) 889
PpsI GAGTC 1 cut(s) 889
PpuMI RGGWCCY 2 cut(s) 46, 564
Psp124BI GAGCTC 1 cut(s) 928
Psp5II RGGWCCY 2 cut(s) 46, 564
Psp6I CCWGG 1 cut(s) 655
PspGI CCWGG 1 cut(s) 655
PspN4I GGNNCC 3 cut(s) 56, 565, 566
PspPI GGNCC 4 cut(s) 46, 54, 113, 564
PspPPI RGGWCCY 2 cut(s) 46, 564
PstI CTGCAG 2 cut(s) 40, 715
PsuI RGATCY 2 cut(s) 346, 543
RseI CAYNNNNRTG 1 cut(s) 618
SacI GAGCTC 1 cut(s) 928
SaqAI TTAA 2 cut(s) 617, 831
SatI GCNGC 3 cut(s) 29, 32, 327
Sau3AI GATC 3 cut(s) 346, 523, 543
Sau96I GGNCC 4 cut(s) 46, 54, 113, 564
SchI GAGTC 1 cut(s) 890
ScrFI CCNGG 2 cut(s) 237, 657
SduI GDGCHC 1 cut(s) 928
SfaNI GCATC 5 cut(s) 124, 412, 588, 610, 826
SfcI CTRYAG 2 cut(s) 36, 711
SfuI TTCGAA 1 cut(s) 515
SinI GGWCC 3 cut(s) 46, 54, 564
SmiI ATTTAAAT 1 cut(s) 618
SmiMI CAYNNNNRTG 1 cut(s) 618
SmlI CTYRAG 1 cut(s) 407
SmoI CTYRAG 1 cut(s) 407
Sse9I AATT 8 cut(s) 138, 144, 389, 633, 666, 773, 898, 910
SsiI CCGC 3 cut(s) 28, 32, 329
SspI AATATT 1 cut(s) 473
SspMI CTAG 5 cut(s) 386, 440, 446, 498, 587
SstI GAGCTC 1 cut(s) 928
StyD4I CCNGG 2 cut(s) 235, 655
SwaI ATTTAAAT 1 cut(s) 618
TaaI ACNGT 1 cut(s) 580
TaqI TCGA 1 cut(s) 515
TasI AATT 8 cut(s) 138, 144, 389, 633, 666, 773, 898, 910
TauI GCSGC 2 cut(s) 31, 34
TfiI GAWTC 5 cut(s) 398, 517, 700, 794, 857
Tru1I TTAA 2 cut(s) 617, 831
Tru9I TTAA 2 cut(s) 617, 831
TscAI CASTG 1 cut(s) 895
TseFI GTSAC 3 cut(s) 11, 535, 737
TseI GCWGC 1 cut(s) 326
Tsp45I GTSAC 3 cut(s) 11, 535, 737
TspDTI ATGAA 1 cut(s) 816
TspRI CASTG 1 cut(s) 895
VpaK11BI GGWCC 3 cut(s) 46, 54, 564
XapI RAATTY 4 cut(s) 389, 773, 898, 910
XbaI TCTAGA 1 cut(s) 586
XceI RCATGY 1 cut(s) 758
XmiI GTMKAC 1 cut(s) 360
XmnI GAANNNNTTC 1 cut(s) 198
XspI CTAG 5 cut(s) 386, 440, 446, 498, 587
Zsp2I ATGCAT 2 cut(s) 603, 841
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.