pycom10g08560

alpha-L-fucosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0004443
Physical Location & Seq
Forward (+)
11300636 .. 11302339
1704 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g08560.1

Sequence Viewer

Length: 600 bp
ATGGAGGATGATGGTGAGTGGGTTCTGGTACGGAGCCCTGCAGAGAAGGACCTGTGGACCCCATCTTTAGTGGAGGAGGAGGAGGGGGAGAGTTCTAGGCCTCTGAAGGTCACATTTTCTGGGCCTGCCAAGCACTGGACTGATGCTATACCAATTGGGAATGGCAGGCTTGGAGCCATGGTTTGGGGTGGTGTGGCATCAGAAACTCTCCAACTCAATGAGGACACACTCTGGACTGGAATTCCTGGCAACTATACCAACCCAAAGGCTCCACAGGTACTAACAGAGGTTAGAAAACTTGTTGACAATGGTAAATATGTCGAAGCTACTGAAGCAGCGGTCAAGTTGGCGGGAGATCCTTCTGATGTTTATCAACCACTCGGTGGCATCAACCTAGAATTCGGTGACACACATCTCAAATATGATGAGGAAACTTACAGCAGAGAGCTAGATCTGGATACTGCAACGGCAAAGATAAATTACTCAGTGGGCGGTGTAGAATATACAAGGGAGCACTTTTCGTCTTATCCTAATCAAGTGATTGTGACAAAGATCGATCCGCTAGCAAACCAGGGTCCCTATCATTTACAGTTTCTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004553 GO:0004560 GO:0005575 GO:0005576 GO:0015928 GO:0016787 GO:0016798 GO:0047513 GO:0048046
KEGG Pathways
Metabolic & Signaling
ko00511 map00511
Pfam Domains
Protein Families
Glyco_hyd_65N_2

Protein Analysis

200

Amino Acids

22.0

Weight (kDa)

4.52

Isoelectric Point (pI)

26.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hyd_65N_2 PF14498 39 - 186 4.1e-51 Glycosyl hydrolase family 65, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 3 cut(s) 135, 183, 383
AciI CCGC 4 cut(s) 338, 350, 492, 560
AclWI GGATC 2 cut(s) 350, 551
AcsI RAATTY 2 cut(s) 240, 398
AcuI CTGAAG 2 cut(s) 125, 351
AdeI CACNNNGTG 1 cut(s) 383
AfaI GTAC 2 cut(s) 30, 279
AfiI CCNNNNNNNGG 3 cut(s) 135, 183, 383
AjnI CCWGG 2 cut(s) 244, 570
AluBI AGCT 2 cut(s) 326, 448
AluI AGCT 2 cut(s) 326, 448
Alw21I GWGCWC 1 cut(s) 516
AlwI GGATC 2 cut(s) 350, 551
AoxI GGCC 2 cut(s) 98, 122
ApeKI GCWGC 1 cut(s) 335
ApoI RAATTY 2 cut(s) 240, 398
AspS9I GGNCC 4 cut(s) 49, 57, 122, 575
AsuHPI GGTGA 2 cut(s) 26, 416
AsuNHI GCTAGC 1 cut(s) 562
AvaII GGWCC 3 cut(s) 49, 57, 575
BanII GRGCYC 1 cut(s) 38
Bbv12I GWGCWC 1 cut(s) 516
BbvI GCAGC 1 cut(s) 347
BccI CCATC 2 cut(s) 5, 70
BceAI ACGGC 1 cut(s) 483
BciT130I CCWGG 2 cut(s) 246, 572
BciVI GTATCC 1 cut(s) 451
BfaI CTAG 5 cut(s) 96, 395, 449, 563, 598
BfmI CTRYAG 1 cut(s) 39
BfuI GTATCC 1 cut(s) 451
BglII AGATCT 1 cut(s) 451
BisI GCNGC 1 cut(s) 336
BlsI GCNGC 1 cut(s) 337
Bme1390I CCNGG 2 cut(s) 246, 572
Bme18I GGWCC 3 cut(s) 49, 57, 575
BmgT120I GGNCC 4 cut(s) 49, 57, 122, 575
BmiI GGNNCC 6 cut(s) 35, 59, 175, 270, 576, 577
BmrFI CCNGG 2 cut(s) 246, 572
BmsI GCATC 3 cut(s) 133, 206, 396
BmtI GCTAGC 1 cut(s) 566
Bsa29I ATCGAT 1 cut(s) 555
BsaBI GATNNNNATC 1 cut(s) 369
BsaJI CCNNGG 2 cut(s) 177, 571
BsaXI ACNNNNNCTCC 2 cut(s) 165, 195
Bsc4I CCNNNNNNNGG 3 cut(s) 135, 183, 383
Bse1I ACTGG 2 cut(s) 140, 241
Bse8I GATNNNNATC 1 cut(s) 369
BseBI CCWGG 2 cut(s) 246, 572
BseCI ATCGAT 1 cut(s) 555
BseDI CCNNGG 2 cut(s) 177, 571
BseGI GGATG 1 cut(s) 13
BseJI GATNNNNATC 1 cut(s) 369
BseLI CCNNNNNNNGG 3 cut(s) 135, 183, 383
BseMII CTCAG 1 cut(s) 498
BseNI ACTGG 2 cut(s) 140, 241
BseRI GAGGAG 3 cut(s) 89, 92, 95
BseXI GCAGC 1 cut(s) 347
BshFI GGCC 2 cut(s) 100, 124
BshVI ATCGAT 1 cut(s) 555
BsiHKAI GWGCWC 1 cut(s) 516
BslFI GGGAC 1 cut(s) 561
BslI CCNNNNNNNGG 3 cut(s) 135, 183, 383
BsmFI GGGAC 1 cut(s) 561
BsnI GGCC 2 cut(s) 100, 124
Bsp1286I GDGCHC 2 cut(s) 38, 516
Bsp143I GATC 4 cut(s) 355, 451, 552, 556
Bsp19I CCATGG 1 cut(s) 177
BspACI CCGC 4 cut(s) 338, 350, 492, 560
BspANI GGCC 2 cut(s) 100, 124
BspCNI CTCAG 1 cut(s) 497
BspDI ATCGAT 1 cut(s) 555
BspLI GGNNCC 6 cut(s) 35, 59, 175, 270, 576, 577
BspMAI CTGCAG 1 cut(s) 43
BspOI GCTAGC 1 cut(s) 566
BspPI GGATC 2 cut(s) 350, 551
BsrI ACTGG 2 cut(s) 140, 241
BssECI CCNNGG 2 cut(s) 177, 571
BssMI GATC 4 cut(s) 355, 451, 552, 556
BssT1I CCWWGG 1 cut(s) 177
Bst2UI CCWGG 2 cut(s) 246, 572
Bst4CI ACNGT 1 cut(s) 591
BstC8I GCNNGC 3 cut(s) 126, 167, 564
BstDEI CTNAG 1 cut(s) 484
BstDSI CCRYGG 1 cut(s) 177
BstF5I GGATG 1 cut(s) 13
BstKTI GATC 4 cut(s) 358, 454, 555, 559
BstMBI GATC 4 cut(s) 355, 451, 552, 556
BstMWI GCNNNNNNNGC 2 cut(s) 130, 332
BstNI CCWGG 2 cut(s) 246, 572
BstSCI CCNGG 2 cut(s) 244, 570
BstSFI CTRYAG 1 cut(s) 39
BstV1I GCAGC 1 cut(s) 347
BstX2I RGATCY 2 cut(s) 355, 451
BstYI RGATCY 2 cut(s) 355, 451
Bsu15I ATCGAT 1 cut(s) 555
BsuI GTATCC 1 cut(s) 451
BsuRI GGCC 2 cut(s) 100, 124
BsuTUI ATCGAT 1 cut(s) 555
BtgI CCRYGG 1 cut(s) 177
BtsCI GGATG 1 cut(s) 13
BtsIMutI CAGTG 2 cut(s) 133, 492
Cac8I GCNNGC 3 cut(s) 126, 167, 564
Cfr13I GGNCC 4 cut(s) 49, 57, 122, 575
ClaI ATCGAT 1 cut(s) 555
Csp6I GTAC 2 cut(s) 29, 278
CviAII CATG 1 cut(s) 178
CviJI RGCY 8 cut(s) 36, 100, 124, 169, 176, 269, 326, 448
CviKI_1 RGCY 8 cut(s) 36, 100, 124, 169, 176, 269, 326, 448
CviQI GTAC 2 cut(s) 29, 278
DdeI CTNAG 1 cut(s) 484
DpnI GATC 4 cut(s) 357, 453, 554, 558
DpnII GATC 4 cut(s) 355, 451, 552, 556
DraIII CACNNNGTG 1 cut(s) 383
Eco130I CCWWGG 1 cut(s) 177
Eco147I AGGCCT 1 cut(s) 100
Eco24I GRGCYC 1 cut(s) 38
Eco47I GGWCC 3 cut(s) 49, 57, 575
Eco57I CTGAAG 2 cut(s) 125, 351
EcoO109I RGGNCCY 2 cut(s) 49, 575
EcoRI GAATTC 2 cut(s) 240, 398
EcoRII CCWGG 2 cut(s) 244, 570
EcoT14I CCWWGG 1 cut(s) 177
EcoT38I GRGCYC 1 cut(s) 38
ErhI CCWWGG 1 cut(s) 177
FaeI CATG 1 cut(s) 181
FaiI YATR 6 cut(s) 149, 179, 255, 318, 423, 504
FaqI GGGAC 1 cut(s) 561
FatI CATG 1 cut(s) 177
FauI CCCGC 1 cut(s) 343
Fnu4HI GCNGC 1 cut(s) 336
FokI GGATG 1 cut(s) 20
FriOI GRGCYC 1 cut(s) 38
Fsp4HI GCNGC 1 cut(s) 336
FspBI CTAG 5 cut(s) 96, 395, 449, 563, 598
GluI GCNGC 1 cut(s) 336
HaeIII GGCC 2 cut(s) 100, 124
Hin1II CATG 1 cut(s) 181
HincII GTYRAC 1 cut(s) 304
HindII GTYRAC 1 cut(s) 304
HphI GGTGA 2 cut(s) 26, 416
Hpy166II GTNNAC 2 cut(s) 57, 304
Hpy188I TCNGA 3 cut(s) 105, 202, 364
Hpy188III TCNNGA 2 cut(s) 232, 455
Hpy8I GTNNAC 2 cut(s) 57, 304
HpyAV CCTTC 3 cut(s) 40, 100, 369
HpyCH4III ACNGT 1 cut(s) 591
HpyCH4V TGCA 2 cut(s) 41, 464
HpyF10VI GCNNNNNNNGC 2 cut(s) 130, 332
HpyF3I CTNAG 1 cut(s) 484
Hsp92II CATG 1 cut(s) 181
KflI GGGWCCC 1 cut(s) 575
Kzo9I GATC 4 cut(s) 355, 451, 552, 556
LmnI GCTCC 4 cut(s) 33, 173, 274, 511
Lsp1109I GCAGC 1 cut(s) 347
LweI GCATC 3 cut(s) 133, 206, 396
MaeI CTAG 5 cut(s) 96, 395, 449, 563, 598
MaeIII GTNAC 3 cut(s) 109, 404, 544
MalI GATC 4 cut(s) 357, 453, 554, 558
MboI GATC 4 cut(s) 355, 451, 552, 556
MfeI CAATTG 1 cut(s) 153
MflI RGATCY 2 cut(s) 355, 451
MhlI GDGCHC 2 cut(s) 38, 516
MluCI AATT 4 cut(s) 153, 240, 398, 478
MmeI TCCRAC 1 cut(s) 235
MnlI CCTC 8 cut(s) 67, 70, 73, 76, 111, 214, 280, 421
MspA1I CMGCKG 1 cut(s) 338
MspR9I CCNGG 2 cut(s) 246, 572
MunI CAATTG 1 cut(s) 153
MvaI CCWGG 2 cut(s) 246, 572
MwoI GCNNNNNNNGC 2 cut(s) 130, 332
NcoI CCATGG 1 cut(s) 177
NdeII GATC 4 cut(s) 355, 451, 552, 556
NheI GCTAGC 1 cut(s) 562
NlaIII CATG 1 cut(s) 181
NlaIV GGNNCC 6 cut(s) 35, 59, 175, 270, 576, 577
NmuCI GTSAC 3 cut(s) 109, 404, 544
PceI AGGCCT 1 cut(s) 100
PflMI CCANNNNNTGG 3 cut(s) 135, 183, 383
PkrI GCNGC 1 cut(s) 337
PpuMI RGGWCCY 2 cut(s) 49, 575
Psp5II RGGWCCY 2 cut(s) 49, 575
Psp6I CCWGG 2 cut(s) 244, 570
PspGI CCWGG 2 cut(s) 244, 570
PspN4I GGNNCC 6 cut(s) 35, 59, 175, 270, 576, 577
PspPI GGNCC 4 cut(s) 49, 57, 122, 575
PspPPI RGGWCCY 2 cut(s) 49, 575
PstI CTGCAG 1 cut(s) 43
PsuI RGATCY 2 cut(s) 355, 451
RsaI GTAC 2 cut(s) 30, 279
RsaNI GTAC 2 cut(s) 29, 278
SatI GCNGC 1 cut(s) 336
Sau3AI GATC 4 cut(s) 355, 451, 552, 556
Sau96I GGNCC 4 cut(s) 49, 57, 122, 575
ScrFI CCNGG 2 cut(s) 246, 572
SduI GDGCHC 2 cut(s) 38, 516
SetI ASST 7 cut(s) 54, 111, 279, 291, 328, 396, 450
SfaNI GCATC 3 cut(s) 133, 206, 396
SfcI CTRYAG 1 cut(s) 39
SinI GGWCC 3 cut(s) 49, 57, 575
Sse9I AATT 4 cut(s) 153, 240, 398, 478
SseBI AGGCCT 1 cut(s) 100
SsiI CCGC 4 cut(s) 338, 350, 492, 560
SspMI CTAG 5 cut(s) 96, 395, 449, 563, 598
StuI AGGCCT 1 cut(s) 100
StyD4I CCNGG 2 cut(s) 244, 570
StyI CCWWGG 1 cut(s) 177
TaaI ACNGT 1 cut(s) 591
TaqI TCGA 2 cut(s) 321, 555
TasI AATT 4 cut(s) 153, 240, 398, 478
TscAI CASTG 2 cut(s) 140, 492
TseFI GTSAC 3 cut(s) 109, 404, 544
TseI GCWGC 1 cut(s) 335
Tsp45I GTSAC 3 cut(s) 109, 404, 544
TspGWI ACGGA 1 cut(s) 46
TspRI CASTG 2 cut(s) 140, 492
Van91I CCANNNNNTGG 3 cut(s) 135, 183, 383
VpaK11BI GGWCC 3 cut(s) 49, 57, 575
XapI RAATTY 2 cut(s) 240, 398
XspI CTAG 5 cut(s) 96, 395, 449, 563, 598
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.