MD10G1099200.v1.1

alpha-L-fucosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0004443
Physical Location & Seq
Forward (+)
16038994 .. 16045264
6271 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1099200.v1.1.491

Sequence Viewer

Length: 2694 bp
ATGCGTGGGATCCCCGTTGCTTTACTACATGCCGTGACATTTAAAAGCCTCAACATACCAAAACGAACCGCACCTCTGTTTTCAGCACTGTCTCCGACCCACTCACTCTTCACTCCATCCACTGCACTTCAAAAATCCACAATTCCTAAACCAACCAAAAATCACAACAAAGTCACAAGCTTTTCTTCTTCTTCTTCTTCTTCTTCTTCTTGTGCAATGGAGGATGATGGTGAGTGGGTTCTGGTGCGGAGCCCTGCAGAGAAGGACCTGTGGACCCCATCTTTAGTGGAGGAGGAGGAGAGTTCTAGGCCTCTGAAGGTCGCATTTTCTGGGCCTGCAAAGCACTGGACTGATGCTATACCAATTGGGAATGGCAGGCTTGGAGCTATGGTTTGGGGTGGTGTGGCATCAGAAACTCTCCAACTCAATGAGGACACACTCTGGACTGGAAATCCTGGTAACTATACCAACCCAAAAGCTCCAGAGGTACTAACAGAGGTTAGAAAACTTGTTGACGATGGTAAATATGTCGAAGCCACTGAAGCAGCCGTTGAGTTGTCGGGAGAAGCTTCTGCTGTTTATCAACCACTCGGTGACATCAACCTAGAATTCGGTGACTCTCATCTCAAATATGATGAAGAAACTTACAGCAGAGAGCTAGATCTGGATACTGCAACAGCAAGGATACAGTACTCAGTGGGCGACGTAGAATATACGAGGGAGCACTTTTCGTCTTATCCTAATCAAGTGATTGTGACAAAGATCTCCGCAAGCAAACCAGGGTCCCTATCATTTACAGTTTCTCTAGACAGCAAGTTACAGCATAATTCAAATGTAAATGGTAATAACCAAATTATACTACAAGGAAGTTGTCCTGGCAATCCAAATGGGATTCTGTTTTCGGCAGTTCTTGATTTGCAGATTAGTGATGGCAGTGGTGCCATACATGTTTTGGATGACAACAAATTAAGGGTTGAAGATTCAGATTGGGCTGTTTTGCTTCTTGTGGCCTCATCATCATTTGATGGACCATTTACTAAGCCCTCTGATTCTAAGAGGGATCCGACTTCAGAGTCTCTCACCGCATTGAATTCAATAAGAAATTTTTCGTATTCGGATCTTTATGCTCATCATTTGGATAACTATCAGAATCTTTTCCATCGTGTCTCGTTGCAGCTTTCTAAGAGCTCCAAGAATAATTTAGGAGATAAAACTTTGGAGGCAAAGAAACGTATGACCAATTTGAATATAAAGGGAAGTGATGATGCCTTCATTTCAACTGCTGACCGGGTGAAATCCTTCAAAACTGATGAAGATCCTTCCTTTGTGGAACTTTTATTCCAGTATGGCCGGTATCTGCTTATTTCTTGTTCACGGGTTGGAACTCAAGTTGCAAACCTGCAGGGTATATGGAGCAAGGATCTTTACCCTCCATGGGATGGCGCTCAGCATCTAAACATAAATCTTCAAATGAATTACTGGCCATCCCTTCCATGTAACCTAAGAGAATGCCAGGAGCCCTTATTTGATTATACATCTTCTTTATCCATCAACGGGAGTAAAACGGCTAAGGTGAACTATGAAGCCAGTGGTTGGGTTGTGCATCAAGTGTCTGACATATGGGCGAAAACATCACCCGATAGAGGTCAAGCCGTGTGGGCTTTATGGCCAATGGGAGGTGCCTGGATTTGTACTCATCCATGGGAACATTTTACTTATACGATGGACAAAGATTTTCTAAAAAACAAGGCATATCCTTTGTTGGAAGGATGTACATCATTTCTGCTAGATTGGTTGATTGAAGGCCGTGGAGGGTATCTTGAAACCAACCCGTCAACTTCTCCGGAGCACATGTTTATTGCTCCTGATGGTAAGCAGGCAAGCGTGAGCTACTCATCAACCATGGACATATCAATCATCCAAGAAGTTTTCTCTGCAATACTGTCTGCGGCTGAGATTTTGGGAAAAACACAGGATGCTCTTGTTGGAAAAGTTCGTGAGGCTCAATCTCGGCTGCCACCCACAAAAATTGCTAAAGATGGTTCTATCATGGAATGGGCACAAGATTTTGAGGACCCAGATGTGCACCATCGACATGTATCACACCTGTTTGGCTTGTATCCAGGACACACAATAACCGTTGAGAAAACTCCAGATCTCAGTAAAGCTGTCGATTATACACTCGTTAAAAGAGGAGAGGAGGGTCCAGGATGGTCGACTACATGGAAGACTGCGTTGTGGGCACGTCTTCACAATAGTCAGCATGCATATCGTATGGTAAAGCATTTGATTGACTTGGTGGATCCAGATCACGAAAGCGATTTTGAAGGAGGACTATACAGTAACTTGTTCACTGCACATCCCCCTTTCCAGATTGACGCCAACTTTGGTTTTTCAGCAGCAGTTGCAGAAATGCTTGTCCAAAGCACTGTCAAGGACCTGTATTTTCTTCCCGCTCTTCCACGGGATAAATGGGCAAACGGTTGTGTGAAAGGACTGAAGGCTCGTGGCGGGGTGACAGTCAATATATGTTGGAAGGAAGGTGACCTTCATGAAGCTGGTCTTTGGTCTAAGGACCATAACACCACGAAAATATTACATTATAGTGGAACTACAGTTATAGCAAACATATCATCTGGCAGGATCTATACTTTCAACAGACAGTTAAAATGTTTGAGGACAACATATCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004553 GO:0004560 GO:0005575 GO:0005576 GO:0015928 GO:0016787 GO:0016798 GO:0047513 GO:0048046
KEGG Pathways
Metabolic & Signaling
ko00511 map00511
Pfam Domains
Protein Families
Glyco_hyd_65N_2

Protein Analysis

898

Amino Acids

99.45

Weight (kDa)

5.77

Isoelectric Point (pI)

33.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hyd_65N_2 PF14498 109 - 343 7.6e-68 Glycosyl hydrolase family 65, N-terminal domain
Glyco_hydro_95_cat PF22124 370 - 806 1.1e-164 Glycosyl hydrolase family 95 catalytic domain
Glyco_hydro_95_C PF21307 808 - 871 3.6e-14 Glycoside hydrolase family 95, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1072
Acc36I ACCTGC 1 cut(s) 1407
AccB1I GGYRCC 2 cut(s) 938, 1679
AccB7I CCANNNNNTGG 2 cut(s) 1439, 1593
AccBSI CCGCTC 1 cut(s) 2456
AccI GTMKAC 1 cut(s) 2216
AccIII TCCGGA 1 cut(s) 1843
AciI CCGC 7 cut(s) 69, 247, 768, 1083, 1949, 2454, 2511
AcoI YGGCCR 3 cut(s) 1348, 1481, 1667
AcsI RAATTY 3 cut(s) 608, 1090, 1102
AcuI CTGAAG 4 cut(s) 335, 561, 1053, 2519
AcyI GRCGYC 1 cut(s) 2379
AdeI CACNNNGTG 1 cut(s) 593
AfaI GTAC 4 cut(s) 489, 692, 1693, 1774
AfiI CCNNNNNNNGG 6 cut(s) 1435, 1439, 1554, 1593, 1643, 1676
AflIII ACRYGT 3 cut(s) 946, 1851, 2095
AjiI CACGTC 1 cut(s) 2246
AjnI CCWGG 7 cut(s) 454, 778, 874, 1512, 1682, 2122, 2206
AloI GAACNNNNNNTCC 2 cut(s) 1323, 1355
Alw21I GWGCWC 4 cut(s) 726, 1190, 1851, 2088
Alw26I GTCTC 3 cut(s) 96, 1080, 1171
Alw44I GTGCAC 1 cut(s) 2084
Aor13HI TCCGGA 1 cut(s) 1843
AoxI GGCC 7 cut(s) 308, 332, 1008, 1348, 1481, 1667, 1804
ApaLI GTGCAC 1 cut(s) 2084
ApeKI GCWGC 4 cut(s) 545, 1174, 2014, 2399
ApoI RAATTY 3 cut(s) 608, 1090, 1102
ArsI GACNNNNNNTTYG 2 cut(s) 2369, 2401
Asp700I GAANNNNTTC 3 cut(s) 1105, 1154, 1298
AspLEI GCGC 1 cut(s) 1445
AspS9I GGNCC 9 cut(s) 265, 273, 332, 783, 1028, 2074, 2204, 2437, 2575
AsuC2I CCSGG 1 cut(s) 1289
AsuHPI GGTGA 9 cut(s) 242, 605, 626, 1072, 1303, 1585, 1626, 2527, 2555
AvaII GGWCC 8 cut(s) 265, 273, 783, 1028, 2074, 2204, 2437, 2575
BaeGI GKGCMC 3 cut(s) 2062, 2088, 2245
BalI TGGCCA 2 cut(s) 1483, 1669
BamHI GGATCC 3 cut(s) 9, 1060, 2302
BanI GGYRCC 2 cut(s) 938, 1679
BanII GRGCYC 3 cut(s) 254, 1190, 1521
BauI CACGAG 1 cut(s) 2505
BbsI GAAGAC 2 cut(s) 2234, 2240
Bbv12I GWGCWC 4 cut(s) 726, 1190, 1851, 2088
BbvI GCAGC 4 cut(s) 557, 1186, 2001, 2411
BceAI ACGGC 5 cut(s) 17, 533, 1581, 1637, 1791
BcgI CGANNNNNNTGC 2 cut(s) 2252, 2286
BciT130I CCWGG 7 cut(s) 456, 780, 876, 1514, 1684, 2124, 2208
BciVI GTATCC 3 cut(s) 661, 678, 2130
BcnI CCSGG 1 cut(s) 1289
BcoDI GTCTC 3 cut(s) 96, 1080, 1171
BfaI CTAG 5 cut(s) 306, 605, 659, 806, 1787
BfmI CTRYAG 3 cut(s) 255, 1400, 2613
BfoI RGCGCY 1 cut(s) 1446
BfuAI ACCTGC 1 cut(s) 1407
BfuI GTATCC 3 cut(s) 661, 678, 2130
BglI GCCNNNNNGGC 1 cut(s) 1658
BglII AGATCT 3 cut(s) 661, 762, 2155
BisI GCNGC 5 cut(s) 546, 1175, 1950, 2015, 2400
BlpI GCTNAGC 1 cut(s) 1446
BlsI GCNGC 5 cut(s) 547, 1176, 1951, 2016, 2401
BmcAI AGTACT 1 cut(s) 692
Bme1390I CCNGG 8 cut(s) 456, 780, 876, 1289, 1514, 1684, 2124, 2208
Bme18I GGWCC 8 cut(s) 265, 273, 783, 1028, 2074, 2204, 2437, 2575
BmgBI CACGTC 1 cut(s) 2246
BmgT120I GGNCC 9 cut(s) 265, 273, 332, 783, 1028, 2074, 2204, 2437, 2575
BmrFI CCNGG 8 cut(s) 456, 780, 876, 1289, 1514, 1684, 2124, 2208
BmsI GCATC 6 cut(s) 343, 416, 1255, 1459, 1612, 1966
BpiI GAAGAC 2 cut(s) 2234, 2240
BpmI CTGGAG 2 cut(s) 465, 2136
Bpu10I CCTNAGC 1 cut(s) 1569
Bpu1102I GCTNAGC 1 cut(s) 1446
BpuEI CTTGAG 1 cut(s) 1371
BpuMI CCSGG 1 cut(s) 1289
BsaBI GATNNNNATC 4 cut(s) 1143, 1314, 1774, 2307
BsaHI GRCGYC 1 cut(s) 2379
BsaJI CCNNGG 6 cut(s) 779, 1433, 1700, 1807, 1902, 2462
BsaWI WCCGGW 1 cut(s) 1843
BsaXI ACNNNNNCTCC 4 cut(s) 375, 405, 1838, 1868
Bsc4I CCNNNNNNNGG 6 cut(s) 1435, 1439, 1554, 1593, 1643, 1676
Bse118I RCCGGY 1 cut(s) 1350
Bse1I ACTGG 5 cut(s) 350, 451, 1342, 1484, 1587
Bse3DI GCAATG 1 cut(s) 222
Bse8I GATNNNNATC 4 cut(s) 1143, 1314, 1774, 2307
BseAI TCCGGA 1 cut(s) 1843
BseBI CCWGG 7 cut(s) 456, 780, 876, 1514, 1684, 2124, 2208
BseDI CCNNGG 6 cut(s) 779, 1433, 1700, 1807, 1902, 2462
BseJI GATNNNNATC 4 cut(s) 1143, 1314, 1774, 2307
BseLI CCNNNNNNNGG 6 cut(s) 1435, 1439, 1554, 1593, 1643, 1676
BseMI GCAATG 1 cut(s) 222
BseMII CTCAG 4 cut(s) 708, 1460, 1944, 2173
BseNI ACTGG 5 cut(s) 350, 451, 1342, 1484, 1587
BseRI GAGGAG 5 cut(s) 305, 308, 311, 2208, 2213
BseSI GKGCMC 3 cut(s) 2062, 2088, 2245
BseXI GCAGC 4 cut(s) 557, 1186, 2001, 2411
BsgI GTGCAG 2 cut(s) 108, 2340
BshFI GGCC 7 cut(s) 310, 334, 1010, 1350, 1483, 1669, 1806
BshNI GGYRCC 2 cut(s) 938, 1679
BsiHKAI GWGCWC 4 cut(s) 726, 1190, 1851, 2088
BsiSI CCGG 3 cut(s) 1288, 1351, 1844
BslFI GGGAC 1 cut(s) 769
BslI CCNNNNNNNGG 6 cut(s) 1435, 1439, 1554, 1593, 1643, 1676
BsmAI GTCTC 3 cut(s) 96, 1080, 1171
BsmFI GGGAC 1 cut(s) 769
BsmI GAATGC 1 cut(s) 1514
BsnI GGCC 7 cut(s) 310, 334, 1010, 1350, 1483, 1669, 1806
Bsp1286I GDGCHC 8 cut(s) 254, 726, 1190, 1521, 1851, 2062, 2088, 2245
Bsp13I TCCGGA 1 cut(s) 1843
Bsp1407I TGTACA 1 cut(s) 1772
Bsp1720I GCTNAGC 1 cut(s) 1446
Bsp19I CCATGG 3 cut(s) 1433, 1700, 1902
BspACI CCGC 7 cut(s) 69, 247, 768, 1083, 1949, 2454, 2511
BspANI GGCC 7 cut(s) 310, 334, 1010, 1350, 1483, 1669, 1806
BspCNI CTCAG 4 cut(s) 707, 1459, 1945, 2172
BspEI TCCGGA 1 cut(s) 1843
BspHI TCATGA 1 cut(s) 2551
BspMAI CTGCAG 2 cut(s) 259, 1404
BspMI ACCTGC 1 cut(s) 1407
BspQI GCTCTTC 1 cut(s) 2463
BspT107I GGYRCC 2 cut(s) 938, 1679
BsrBI CCGCTC 1 cut(s) 2456
BsrDI GCAATG 1 cut(s) 222
BsrFI RCCGGY 1 cut(s) 1350
BsrGI TGTACA 1 cut(s) 1772
BsrI ACTGG 5 cut(s) 350, 451, 1342, 1484, 1587
BssAI RCCGGY 1 cut(s) 1350
BssECI CCNNGG 6 cut(s) 779, 1433, 1700, 1807, 1902, 2462
BssNI GRCGYC 1 cut(s) 2379
BssSI CACGAG 1 cut(s) 2505
BssT1I CCWWGG 3 cut(s) 1433, 1700, 1902
Bst2BI CACGAG 1 cut(s) 2505
Bst2UI CCWGG 7 cut(s) 456, 780, 876, 1514, 1684, 2124, 2208
Bst6I CTCTTC 2 cut(s) 113, 2463
BstACI GRCGYC 1 cut(s) 2379
BstAPI GCANNNNNTGC 1 cut(s) 2405
BstAUI TGTACA 1 cut(s) 1772
BstC8I GCNNGC 6 cut(s) 336, 377, 772, 1878, 1882, 2265
BstDSI CCRYGG 5 cut(s) 1433, 1700, 1807, 1902, 2462
BstEII GGTNACC 1 cut(s) 2543
BstH2I RGCGCY 1 cut(s) 1446
BstHHI GCGC 1 cut(s) 1445
BstMAI GTCTC 3 cut(s) 96, 1080, 1171
BstMWI GCNNNNNNNGC 5 cut(s) 340, 542, 1658, 2240, 2405
BstNI CCWGG 7 cut(s) 456, 780, 876, 1514, 1684, 2124, 2208
BstNSI RCATGY 5 cut(s) 32, 950, 1855, 2099, 2267
BstPI GGTNACC 1 cut(s) 2543
BstSCI CCNGG 8 cut(s) 454, 778, 874, 1287, 1512, 1682, 2122, 2206
BstSFI CTRYAG 3 cut(s) 255, 1400, 2613
BstSLI GKGCMC 3 cut(s) 2062, 2088, 2245
BstV1I GCAGC 4 cut(s) 557, 1186, 2001, 2411
BstV2I GAAGAC 2 cut(s) 2234, 2240
BsuI GTATCC 3 cut(s) 661, 678, 2130
BsuRI GGCC 7 cut(s) 310, 334, 1010, 1350, 1483, 1669, 1806
BtgI CCRYGG 5 cut(s) 1433, 1700, 1807, 1902, 2462
BtrI CACGTC 1 cut(s) 2246
BtsI GCAGTG 3 cut(s) 120, 940, 2352
BtsIMutI CAGTG 9 cut(s) 86, 120, 343, 537, 702, 940, 1594, 2352, 2427
BveI ACCTGC 1 cut(s) 1407
Cac8I GCNNGC 6 cut(s) 336, 377, 772, 1878, 1882, 2265
CciI TCATGA 1 cut(s) 2551
CfoI GCGC 1 cut(s) 1445
Cfr10I RCCGGY 1 cut(s) 1350
Cfr13I GGNCC 9 cut(s) 265, 273, 332, 783, 1028, 2074, 2204, 2437, 2575
CseI GACGC 1 cut(s) 2387
Csp6I GTAC 4 cut(s) 488, 691, 1692, 1773
CspCI CAANNNNNGTGG 4 cut(s) 1637, 1672, 2011, 2046
CviQI GTAC 4 cut(s) 488, 691, 1692, 1773
DraI TTTAAA 1 cut(s) 43
DraIII CACNNNGTG 1 cut(s) 593
DrdI GACNNNNNNGTC 1 cut(s) 1072
DseDI GACNNNNNNGTC 1 cut(s) 1072
EaeI YGGCCR 3 cut(s) 1348, 1481, 1667
Eam1104I CTCTTC 2 cut(s) 113, 2463
EarI CTCTTC 2 cut(s) 113, 2463
Ecl136II GAGCTC 1 cut(s) 1188
Eco130I CCWWGG 3 cut(s) 1433, 1700, 1902
Eco147I AGGCCT 1 cut(s) 310
Eco24I GRGCYC 3 cut(s) 254, 1190, 1521
Eco47I GGWCC 8 cut(s) 265, 273, 783, 1028, 2074, 2204, 2437, 2575
Eco53kI GAGCTC 1 cut(s) 1188
Eco57I CTGAAG 4 cut(s) 335, 561, 1053, 2519
Eco91I GGTNACC 1 cut(s) 2543
EcoICRI GAGCTC 1 cut(s) 1188
EcoO109I RGGNCCY 4 cut(s) 265, 783, 2074, 2437
EcoO65I GGTNACC 1 cut(s) 2543
EcoRI GAATTC 2 cut(s) 608, 1090
EcoRII CCWGG 7 cut(s) 454, 778, 874, 1512, 1682, 2122, 2206
EcoT14I CCWWGG 3 cut(s) 1433, 1700, 1902
EcoT22I ATGCAT 1 cut(s) 2269
EcoT38I GRGCYC 3 cut(s) 254, 1190, 1521
ErhI CCWWGG 3 cut(s) 1433, 1700, 1902
FalI AAGNNNNNCTT 6 cut(s) 169, 201, 2532, 2564, 2547, 2579
FaqI GGGAC 1 cut(s) 769
FauI CCCGC 2 cut(s) 2461, 2504
FauNDI CATATG 1 cut(s) 1619
FblI GTMKAC 1 cut(s) 2216
Fnu4HI GCNGC 5 cut(s) 546, 1175, 1950, 2015, 2400
FriOI GRGCYC 3 cut(s) 254, 1190, 1521
Fsp4HI GCNGC 5 cut(s) 546, 1175, 1950, 2015, 2400
FspBI CTAG 5 cut(s) 306, 605, 659, 806, 1787
GlaI GCGC 1 cut(s) 1444
GluI GCNGC 5 cut(s) 546, 1175, 1950, 2015, 2400
GsuI CTGGAG 2 cut(s) 465, 2136
HaeII RGCGCY 1 cut(s) 1446
HaeIII GGCC 7 cut(s) 310, 334, 1010, 1350, 1483, 1669, 1806
HapII CCGG 3 cut(s) 1288, 1351, 1844
HgaI GACGC 1 cut(s) 2387
HhaI GCGC 1 cut(s) 1445
Hin1I GRCGYC 1 cut(s) 2379
Hin6I GCGC 1 cut(s) 1443
HinP1I GCGC 1 cut(s) 1443
HincII GTYRAC 3 cut(s) 514, 1836, 2217
HindII GTYRAC 3 cut(s) 514, 1836, 2217
HindIII AAGCTT 2 cut(s) 178, 567
HinfI GANTC 6 cut(s) 617, 892, 980, 1049, 1073, 1150
HpaII CCGG 3 cut(s) 1288, 1351, 1844
HphI GGTGA 9 cut(s) 242, 605, 626, 1072, 1303, 1585, 1626, 2527, 2555
Hpy166II GTNNAC 8 cut(s) 273, 514, 1373, 1576, 1836, 2086, 2217, 2352
Hpy8I GTNNAC 8 cut(s) 273, 514, 1373, 1576, 1836, 2086, 2217, 2352
Hpy99I CGWCG 1 cut(s) 707
HpyCH4IV ACGT 3 cut(s) 705, 1231, 2245
HpyF10VI GCNNNNNNNGC 5 cut(s) 340, 542, 1658, 2240, 2405
HpySE526I ACGT 3 cut(s) 705, 1231, 2245
Hsp92I GRCGYC 1 cut(s) 2379
HspAI GCGC 1 cut(s) 1443
KflI GGGWCCC 1 cut(s) 783
Kpn2I TCCGGA 1 cut(s) 1843
LguI GCTCTTC 1 cut(s) 2463
LmnI GCTCC 9 cut(s) 249, 383, 484, 721, 1193, 1413, 1516, 1846, 1867
Lsp1109I GCAGC 4 cut(s) 557, 1186, 2001, 2411
LweI GCATC 6 cut(s) 343, 416, 1255, 1459, 1612, 1966
MaeI CTAG 5 cut(s) 306, 605, 659, 806, 1787
MaeII ACGT 3 cut(s) 705, 1231, 2245
MbiI CCGCTC 1 cut(s) 2456
MfeI CAATTG 1 cut(s) 363
MhlI GDGCHC 8 cut(s) 254, 726, 1190, 1521, 1851, 2062, 2088, 2245
MlsI TGGCCA 2 cut(s) 1483, 1669
MluNI TGGCCA 2 cut(s) 1483, 1669
MlyI GAGTC 2 cut(s) 611, 1082
MmeI TCCRAC 7 cut(s) 119, 445, 1088, 1360, 1743, 1966, 2513
Mox20I TGGCCA 2 cut(s) 1483, 1669
Mph1103I ATGCAT 1 cut(s) 2269
MroI TCCGGA 1 cut(s) 1843
MroXI GAANNNNTTC 3 cut(s) 1105, 1154, 1298
MscI TGGCCA 2 cut(s) 1483, 1669
MseI TTAA 4 cut(s) 42, 968, 2187, 2666
MslI CAYNNNNRTG 2 cut(s) 2094, 2604
Msp20I TGGCCA 2 cut(s) 1483, 1669
MspI CCGG 3 cut(s) 1288, 1351, 1844
MspR9I CCNGG 8 cut(s) 456, 780, 876, 1289, 1514, 1684, 2124, 2208
MunI CAATTG 1 cut(s) 363
Mva1269I GAATGC 1 cut(s) 1514
MvaI CCWGG 7 cut(s) 456, 780, 876, 1514, 1684, 2124, 2208
MwoI GCNNNNNNNGC 5 cut(s) 340, 542, 1658, 2240, 2405
NciI CCSGG 1 cut(s) 1289
NcoI CCATGG 3 cut(s) 1433, 1700, 1902
NdeI CATATG 1 cut(s) 1619
NmeAIII GCCGAG 1 cut(s) 1990
NmuCI GTSAC 7 cut(s) 34, 172, 593, 614, 754, 2515, 2543
NsiI ATGCAT 1 cut(s) 2269
NspI RCATGY 5 cut(s) 32, 950, 1855, 2099, 2267
PaeI GCATGC 1 cut(s) 2267
PagI TCATGA 1 cut(s) 2551
PceI AGGCCT 1 cut(s) 310
PciI ACATGT 3 cut(s) 946, 1851, 2095
PciSI GCTCTTC 1 cut(s) 2463
PctI GAATGC 1 cut(s) 1514
PdmI GAANNNNTTC 3 cut(s) 1105, 1154, 1298
PfeI GAWTC 4 cut(s) 892, 980, 1049, 1150
PflMI CCANNNNNTGG 2 cut(s) 1439, 1593
PfoI TCCNGGA 2 cut(s) 2122, 2206
PkrI GCNGC 5 cut(s) 547, 1176, 1951, 2016, 2401
PleI GAGTC 2 cut(s) 611, 1081
PpsI GAGTC 2 cut(s) 611, 1081
PpuMI RGGWCCY 4 cut(s) 265, 783, 2074, 2437
PscI ACATGT 3 cut(s) 946, 1851, 2095
Psp124BI GAGCTC 1 cut(s) 1190
Psp5II RGGWCCY 4 cut(s) 265, 783, 2074, 2437
Psp6I CCWGG 7 cut(s) 454, 778, 874, 1512, 1682, 2122, 2206
PspEI GGTNACC 1 cut(s) 2543
PspGI CCWGG 7 cut(s) 454, 778, 874, 1512, 1682, 2122, 2206
PspPI GGNCC 9 cut(s) 265, 273, 332, 783, 1028, 2074, 2204, 2437, 2575
PspPPI RGGWCCY 4 cut(s) 265, 783, 2074, 2437
PstI CTGCAG 2 cut(s) 259, 1404
RsaI GTAC 4 cut(s) 489, 692, 1693, 1774
RsaNI GTAC 4 cut(s) 488, 691, 1692, 1773
RseI CAYNNNNRTG 2 cut(s) 2094, 2604
SacI GAGCTC 1 cut(s) 1190
SalI GTCGAC 1 cut(s) 2215
SapI GCTCTTC 1 cut(s) 2463
SaqAI TTAA 4 cut(s) 42, 968, 2187, 2666
SatI GCNGC 5 cut(s) 546, 1175, 1950, 2015, 2400
Sau96I GGNCC 9 cut(s) 265, 273, 332, 783, 1028, 2074, 2204, 2437, 2575
SbfI CCTGCAGG 1 cut(s) 1404
ScaI AGTACT 1 cut(s) 692
SchI GAGTC 2 cut(s) 611, 1082
ScrFI CCNGG 8 cut(s) 456, 780, 876, 1289, 1514, 1684, 2124, 2208
SdaI CCTGCAGG 1 cut(s) 1404
SduI GDGCHC 8 cut(s) 254, 726, 1190, 1521, 1851, 2062, 2088, 2245
SfaNI GCATC 6 cut(s) 343, 416, 1255, 1459, 1612, 1966
SfcI CTRYAG 3 cut(s) 255, 1400, 2613
SinI GGWCC 8 cut(s) 265, 273, 783, 1028, 2074, 2204, 2437, 2575
SmiMI CAYNNNNRTG 2 cut(s) 2094, 2604
SmlI CTYRAG 1 cut(s) 1386
SmoI CTYRAG 1 cut(s) 1386
SphI GCATGC 1 cut(s) 2267
Sse8387I CCTGCAGG 1 cut(s) 1404
SseBI AGGCCT 1 cut(s) 310
SsiI CCGC 7 cut(s) 69, 247, 768, 1083, 1949, 2454, 2511
SspI AATATT 1 cut(s) 2595
SspMI CTAG 5 cut(s) 306, 605, 659, 806, 1787
SstI GAGCTC 1 cut(s) 1190
StuI AGGCCT 1 cut(s) 310
StyD4I CCNGG 8 cut(s) 454, 778, 874, 1287, 1512, 1682, 2122, 2206
StyI CCWWGG 3 cut(s) 1433, 1700, 1902
TaiI ACGT 3 cut(s) 708, 1234, 2248
TaqI TCGA 4 cut(s) 531, 2092, 2172, 2216
TatI WGTACW 3 cut(s) 690, 1691, 1772
TauI GCSGC 1 cut(s) 1952
TfiI GAWTC 4 cut(s) 892, 980, 1049, 1150
Tru1I TTAA 4 cut(s) 42, 968, 2187, 2666
Tru9I TTAA 4 cut(s) 42, 968, 2187, 2666
TscAI CASTG 9 cut(s) 93, 127, 350, 544, 702, 940, 1594, 2359, 2434
TseFI GTSAC 7 cut(s) 34, 172, 593, 614, 754, 2515, 2543
TseI GCWGC 4 cut(s) 545, 1174, 2014, 2399
Tsp45I GTSAC 7 cut(s) 34, 172, 593, 614, 754, 2515, 2543
TspDTI ATGAA 7 cut(s) 651, 1261, 1326, 1487, 1596, 2540, 2568
TspRI CASTG 9 cut(s) 93, 127, 350, 544, 702, 940, 1594, 2359, 2434
Van91I CCANNNNNTGG 2 cut(s) 1439, 1593
VneI GTGCAC 1 cut(s) 2084
VpaK11BI GGWCC 8 cut(s) 265, 273, 783, 1028, 2074, 2204, 2437, 2575
XapI RAATTY 3 cut(s) 608, 1090, 1102
XbaI TCTAGA 1 cut(s) 805
XceI RCATGY 5 cut(s) 32, 950, 1855, 2099, 2267
XcmI CCANNNNNNNNNTGG 2 cut(s) 949, 2469
XmiI GTMKAC 1 cut(s) 2216
XmnI GAANNNNTTC 3 cut(s) 1105, 1154, 1298
XspI CTAG 5 cut(s) 306, 605, 659, 806, 1787
ZrmI AGTACT 1 cut(s) 692
Zsp2I ATGCAT 1 cut(s) 2269
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.