Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0004443

Physical Location & Seq

Genomic Coordinates Forward (+)

16096700 .. 16101147

Gene Structure 4448 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1099400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2037 bp

ATGAAGGATGATGGCGAGTGGGTTCTGGTACGGAGCCCTGCAGAGAAGGACCTGTGGACCCCATCTTTAGTGGAGGAGGAGGAGGGGGAGAGTTCTAGGCCTCTGAAGGTCACATTTTCCGGGCCTGCAAAGCACTGGACTGATGCTATACCAATTGGGAATGGCAGGCTTGGAGCCATGGTTTGGGGTGGTGTGGCATCAGAAACTCTCCAACTCAATGAGGACACACTATGGACTGGAATTCCTGGCAACTATACCAACCCAAAGGCTCCACAGGTACTAACAGAGGTTAGAAAACTTGTTGACGATGGTAAATATGTCGAAGCTACTGCAGCAGCGGTCAAGTTGGCTGGAGATCCTTCTGATGTTTATCAACCACTTGGTGACATCAACCTAGAATTCGGTGACTCACATCTCAAATATGATGAAGAAACTTACAGCAGAGAGCTAGATCTGGATACTGCAACAGCAAAGATAAATTACTCAGTGAGCGGTGTAGAATATACAAGGGAGCACTTTTCATCTTATCCTAATCAAGTGATTGTGACAAAGATCTCCGCTAGCAAACCAGGGTCCCTATCATTTACAGTTTCTCTAGACAGCAAGTTACATCATAATTCACATGTAAATGGTAATAACCAAATTATACTACAAGGAAGTTGTCCTGGCAAAAGGATACCACCAAAATCTAATGACAATCCAAAGGGGATTCAGTTTTCGGCAGTTCTTGATTTGCAGATTAGCGATGGCAGTGGTGCAATACATGTTTTGGATGACAAGAAATTAAGGGTTGAAGATTCAGATTGGGCTGTTTTGCTTCTGGCGGCCTCATCATCATTTGATGGACCATTTACTAAGCCCTCTGATTCTAAGAGGGATCCGACTTCAGAGTCTCTCACTGCATTGAATTCAATAAGAAATTCATCGTATTCAGATCTTTATGCTCACCATTTGGAGGACTATCAGAATCTTTTCCATCGTGTCTCATTGCAGCTTTCTAAGAGCTCCAAGAGCAATTTAGGAGATAAAACTTTGGAGGCAAAGAAAAGTATGACCAATTTGAATATAAAGGGAAGTGATGATGCCTTAGTTTCAACAGCAGACCGGGTGAAATCCTTCAAAACTGATGAAGATCCTTCCTTTGTGGAACTTTTATTCCAGTATGGCCGGTATCTGCTTATTTCTTGTTCACGGGTTGGAACTCAAGTGGCAAACCTGCAGGGTATATGGAGCAAGGATATTGAGCCTCCATGGGATGGCGCTCAGCATCTGAACATAAATCTTCAAATGAACTATTGGCCATCCCTTCCCTGTAACCTAAGAGAATGCCAGGAGCCCTTATTTGATTATACATCTTCGTTATCTATCAACGGGAGTAAAACGGCTAAGGTGAACTACGAAGCCAGTGGTTGGGTTGTACATCAAGTGTCTGACATATGGGTGAAAACATCACCTGATCGAGGTCAAGCTGTGTGGGCTTTATGGCCAATGGGAGGAGCCTGGCTTTGTACTCATCTATGGGAACATTTTACTTATACGATGGACAAGGATTTTCTTAAAAATAAGACATATCCTTTGTTGGAAGGATGTACATCATTTCTGCTGGATTGGTTGATTGAAGGCCGTGGAGGGTATCTTGAAACCAACCCGTCAACTTCTCCGGAGCACATGTTTATTGCTCCTGATGGCAAGCAGGCAAGCGTGAGCTACTCGTCAACTATGGACATATCGATCATCAAAGAAGTTTTCTCTGCAATAGTTTCTGCTGCTGAGATTTTAGGAAAAACACAGGATGCTCTTGTTGGAAAAGTTCGTGAGGCTCAGTCACGGTTGCCACCCACAAAAATTGCTAGAGATGGTTCTATCATGGAATGGGCACAAGATTTTGAGGACCCAGAAGTGCACCATCGACATGTATCACACCTGTTTGGCTTGTATCCAGGACACATAATAAGCGTTGAGAAAACTCCAGATCTCTGTAAAGCGGTCGGTTATACGCTCTTTAAAAGAGGTTTGTACATTATGATTGATAACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004553 GO:0004560 GO:0005575 GO:0005576 GO:0015928 GO:0016787 GO:0016798 GO:0047513 GO:0048046

KEGG Pathways

Metabolic & Signaling

ko00511 map00511

Pfam Domains

Protein Families

Glyco_hyd_65N_2

Protein Analysis

679

Amino Acids

75.24

Weight (kDa)

5.34

Isoelectric Point (pI)

31.5

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hyd_65N_2	PF14498	39 - 282	8e-67	Glycosyl hydrolase family 65, N-terminal domain
Glyco_hydro_95_cat	PF22124	309 - 671	8.7e-135	Glycosyl hydrolase family 95 catalytic domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004443)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G34260
fragaria_vesca	FvH4_2g04810 FvH4_2g04810 FvH4_2g04810 FvH4_2g04810 FvH4_2g04870 FvH4_2g04870 FvH4_2g04870 FvH4_2g04870
malus_domestica	MD10G1099200.v1.1 MD10G1099400.v1.1
prunus_persica	Prupe.8G137300_v2.0.a1 Prupe.8G137300_v2.0.a1 Prupe.8G137300_v2.0.a1
pyrus_communis	pycom10g08550 pycom10g08560 pycom10g08570
rosa_chinensis	RchiOBHm_Chr6g0254541 RchiOBHm_Chr6g0254581
rosa_laevigata	RLG00000014903 RLG00000014905
rosa_multiflora	Rmu_co8517753.1_g000001 Rmu_sc0008789.1_g000011 Rmu_sc0009578.1_g000012
rosa_roxburghii	Rroxscaffold_7G00210690
rosa_rugosa	Rorug05G0553300
rosa_samantha	Rh6AG070200 Rh6BG062700 Rh6CG062600 Rh6DG059600
rosa_wichuraiana	Rw6G006150 Rw6G006190

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	889
Acc36I	ACCTGC	1 cut(s)	1224
AccB7I	CCANNNNNTGG	3 cut(s)	183, 1256, 1410
AccBSI	CCGCTC	1 cut(s)	492
AccIII	TCCGGA	1 cut(s)	1660
AciI	CCGC	5 cut(s)	338, 492, 558, 824, 1985
AclWI	GGATC	4 cut(s)	350, 872, 885, 1127
AcoI	YGGCCR	3 cut(s)	1165, 1298, 1484
AcsI	RAATTY	4 cut(s)	240, 398, 907, 919
AcuI	CTGAAG	2 cut(s)	125, 870
AdeI	CACNNNGTG	1 cut(s)	383
AfaI	GTAC	6 cut(s)	30, 279, 1419, 1510, 1591, 2018
AfiI	CCNNNNNNNGG	6 cut(s)	183, 955, 1256, 1410, 1460, 1493
AflIII	ACRYGT	4 cut(s)	622, 763, 1668, 1912
AgsI	TTSAA	9 cut(s)	794, 907, 912, 1063, 1095, 1120, 1286, 1619, 1640
AjnI	CCWGG	6 cut(s)	244, 568, 664, 1329, 1499, 1939
AloI	GAACNNNNNNTCC	2 cut(s)	1140, 1172
AluBI	AGCT	6 cut(s)	326, 448, 994, 1005, 1469, 1707
AluI	AGCT	6 cut(s)	326, 448, 994, 1005, 1469, 1707
Alw21I	GWGCWC	4 cut(s)	516, 1007, 1668, 1905
Alw26I	GTCTC	2 cut(s)	897, 988
Alw44I	GTGCAC	1 cut(s)	1901
AlwI	GGATC	4 cut(s)	350, 872, 885, 1127
AlwNI	CAGNNNCTG	1 cut(s)	1270
Aor13HI	TCCGGA	1 cut(s)	1660
AoxI	GGCC	7 cut(s)	98, 122, 825, 1165, 1298, 1484, 1621
ApaLI	GTGCAC	1 cut(s)	1901
ApeKI	GCWGC	4 cut(s)	332, 335, 991, 1766
ApoI	RAATTY	4 cut(s)	240, 398, 907, 919
ArsI	GACNNNNNNTTYG	2 cut(s)	1558, 1590
Asp700I	GAANNNNTTC	2 cut(s)	971, 1115
AspLEI	GCGC	1 cut(s)	1262
AspS9I	GGNCC	6 cut(s)	49, 57, 122, 573, 845, 1891
AsuC2I	CCSGG	2 cut(s)	121, 1106
AsuHPI	GGTGA	7 cut(s)	395, 416, 938, 1120, 1402, 1443, 1453
AsuNHI	GCTAGC	1 cut(s)	560
AvaII	GGWCC	5 cut(s)	49, 57, 573, 845, 1891
BaeGI	GKGCMC	2 cut(s)	1879, 1905
BalI	TGGCCA	2 cut(s)	1300, 1486
BamHI	GGATCC	1 cut(s)	877
BanII	GRGCYC	3 cut(s)	38, 1007, 1338
Bbv12I	GWGCWC	4 cut(s)	516, 1007, 1668, 1905
BbvI	GCAGC	4 cut(s)	344, 347, 1003, 1753
BceAI	ACGGC	2 cut(s)	1398, 1608
BcgI	CGANNNNNNTGC	2 cut(s)	311, 345
BciT130I	CCWGG	6 cut(s)	246, 570, 666, 1331, 1501, 1941
BciVI	GTATCC	3 cut(s)	451, 669, 1947
BcnI	CCSGG	2 cut(s)	121, 1106
BcoDI	GTCTC	2 cut(s)	897, 988
BfaI	CTAG	6 cut(s)	96, 395, 449, 561, 596, 1851
BfmI	CTRYAG	3 cut(s)	39, 330, 1217
BfoI	RGCGCY	1 cut(s)	1263
BfuAI	ACCTGC	1 cut(s)	1224
BfuI	GTATCC	3 cut(s)	451, 669, 1947
BglII	AGATCT	4 cut(s)	451, 552, 934, 1972
BisI	GCNGC	5 cut(s)	333, 336, 825, 992, 1767
BlpI	GCTNAGC	1 cut(s)	1263
BlsI	GCNGC	5 cut(s)	334, 337, 826, 993, 1768
Bme1390I	CCNGG	8 cut(s)	121, 246, 570, 666, 1106, 1331, 1501, 1941
Bme18I	GGWCC	5 cut(s)	49, 57, 573, 845, 1891
BmgT120I	GGNCC	6 cut(s)	49, 57, 122, 573, 845, 1891
BmrFI	CCNGG	8 cut(s)	121, 246, 570, 666, 1106, 1331, 1501, 1941
BmsI	GCATC	5 cut(s)	133, 206, 1072, 1276, 1783
BmtI	GCTAGC	1 cut(s)	564
BpmI	CTGGAG	2 cut(s)	372, 1953
Bpu10I	CCTNAGC	1 cut(s)	1386
Bpu1102I	GCTNAGC	1 cut(s)	1263
BpuEI	CTTGAG	1 cut(s)	1188
BpuMI	CCSGG	2 cut(s)	121, 1106
Bsa29I	ATCGAT	1 cut(s)	1730
BsaBI	GATNNNNATC	3 cut(s)	369, 1131, 1591
BsaJI	CCNNGG	4 cut(s)	177, 569, 1250, 1624
BsaWI	WCCGGW	1 cut(s)	1660
BsaXI	ACNNNNNCTCC	4 cut(s)	165, 195, 1655, 1685
Bsc4I	CCNNNNNNNGG	6 cut(s)	183, 955, 1256, 1410, 1460, 1493
Bse118I	RCCGGY	1 cut(s)	1167
Bse1I	ACTGG	4 cut(s)	140, 241, 1159, 1404
Bse3DI	GCAATG	1 cut(s)	986
Bse8I	GATNNNNATC	3 cut(s)	369, 1131, 1591
BseAI	TCCGGA	1 cut(s)	1660
BseBI	CCWGG	6 cut(s)	246, 570, 666, 1331, 1501, 1941
BseCI	ATCGAT	1 cut(s)	1730
BseDI	CCNNGG	4 cut(s)	177, 569, 1250, 1624
BseGI	GGATG	6 cut(s)	13, 778, 1261, 1301, 1592, 1798
BseJI	GATNNNNATC	3 cut(s)	369, 1131, 1591
BseLI	CCNNNNNNNGG	6 cut(s)	183, 955, 1256, 1410, 1460, 1493
BseMI	GCAATG	1 cut(s)	986
BseMII	CTCAG	4 cut(s)	498, 1277, 1761, 1835
BseNI	ACTGG	4 cut(s)	140, 241, 1159, 1404
BseRI	GAGGAG	4 cut(s)	89, 92, 95, 1509
BseSI	GKGCMC	2 cut(s)	1879, 1905
BseXI	GCAGC	4 cut(s)	344, 347, 1003, 1753
Bsh1285I	CGRYCG	1 cut(s)	1989
BshFI	GGCC	7 cut(s)	100, 124, 827, 1167, 1300, 1486, 1623
BshVI	ATCGAT	1 cut(s)	1730
BsiEI	CGRYCG	1 cut(s)	1989
BsiHKAI	GWGCWC	4 cut(s)	516, 1007, 1668, 1905
BsiSI	CCGG	4 cut(s)	120, 1105, 1168, 1661
BslFI	GGGAC	1 cut(s)	559
BslI	CCNNNNNNNGG	6 cut(s)	183, 955, 1256, 1410, 1460, 1493
BsmAI	GTCTC	2 cut(s)	897, 988
BsmFI	GGGAC	1 cut(s)	559
BsmI	GAATGC	1 cut(s)	1331
BsnI	GGCC	7 cut(s)	100, 124, 827, 1167, 1300, 1486, 1623
Bsp1286I	GDGCHC	7 cut(s)	38, 516, 1007, 1338, 1668, 1879, 1905
Bsp13I	TCCGGA	1 cut(s)	1660
Bsp1407I	TGTACA	3 cut(s)	1417, 1589, 2016
Bsp143I	GATC	9 cut(s)	355, 451, 552, 877, 934, 1132, 1456, 1731, 1972
Bsp1720I	GCTNAGC	1 cut(s)	1263
Bsp19I	CCATGG	2 cut(s)	177, 1250
BspACI	CCGC	5 cut(s)	338, 492, 558, 824, 1985
BspANI	GGCC	7 cut(s)	100, 124, 827, 1167, 1300, 1486, 1623
BspCNI	CTCAG	4 cut(s)	497, 1276, 1762, 1834
BspDI	ATCGAT	1 cut(s)	1730
BspEI	TCCGGA	1 cut(s)	1660
BspMAI	CTGCAG	3 cut(s)	43, 334, 1221
BspMI	ACCTGC	1 cut(s)	1224
BspOI	GCTAGC	1 cut(s)	564
BspPI	GGATC	4 cut(s)	350, 872, 885, 1127
BsrBI	CCGCTC	1 cut(s)	492
BsrDI	GCAATG	1 cut(s)	986
BsrFI	RCCGGY	1 cut(s)	1167
BsrGI	TGTACA	3 cut(s)	1417, 1589, 2016
BsrI	ACTGG	4 cut(s)	140, 241, 1159, 1404
BssAI	RCCGGY	1 cut(s)	1167
BssECI	CCNNGG	4 cut(s)	177, 569, 1250, 1624
BssMI	GATC	9 cut(s)	355, 451, 552, 877, 934, 1132, 1456, 1731, 1972
BssT1I	CCWWGG	2 cut(s)	177, 1250
Bst2UI	CCWGG	6 cut(s)	246, 570, 666, 1331, 1501, 1941
Bst4CI	ACNGT	2 cut(s)	589, 1830
BstAUI	TGTACA	3 cut(s)	1417, 1589, 2016
BstC8I	GCNNGC	6 cut(s)	126, 167, 562, 1691, 1695, 1699
BstDSI	CCRYGG	3 cut(s)	177, 1250, 1624
BstENI	CCTNNNNNAGG	1 cut(s)	1458
BstF5I	GGATG	6 cut(s)	13, 778, 1261, 1301, 1592, 1798
BstH2I	RGCGCY	1 cut(s)	1263
BstHHI	GCGC	1 cut(s)	1262
BstKTI	GATC	9 cut(s)	358, 454, 555, 880, 937, 1135, 1459, 1734, 1975
BstMAI	GTCTC	2 cut(s)	897, 988
BstMBI	GATC	9 cut(s)	355, 451, 552, 877, 934, 1132, 1456, 1731, 1972
BstMCI	CGRYCG	1 cut(s)	1989
BstMWI	GCNNNNNNNGC	4 cut(s)	130, 332, 1011, 1475
BstNI	CCWGG	6 cut(s)	246, 570, 666, 1331, 1501, 1941
BstNSI	RCATGY	4 cut(s)	626, 767, 1672, 1916
BstSCI	CCNGG	8 cut(s)	119, 244, 568, 664, 1104, 1329, 1499, 1939
BstSFI	CTRYAG	3 cut(s)	39, 330, 1217
BstSLI	GKGCMC	2 cut(s)	1879, 1905
BstV1I	GCAGC	4 cut(s)	344, 347, 1003, 1753
BstX2I	RGATCY	7 cut(s)	355, 451, 552, 877, 934, 1132, 1972
BstYI	RGATCY	7 cut(s)	355, 451, 552, 877, 934, 1132, 1972
Bsu15I	ATCGAT	1 cut(s)	1730
BsuI	GTATCC	3 cut(s)	451, 669, 1947
BsuRI	GGCC	7 cut(s)	100, 124, 827, 1167, 1300, 1486, 1623
BsuTUI	ATCGAT	1 cut(s)	1730
BtgI	CCRYGG	3 cut(s)	177, 1250, 1624
BtgZI	GCGATG	1 cut(s)	759
BtsCI	GGATG	6 cut(s)	13, 778, 1261, 1301, 1592, 1798
BtsI	GCAGTG	2 cut(s)	757, 897
BtsIMutI	CAGTG	5 cut(s)	133, 492, 757, 897, 1411
BveI	ACCTGC	1 cut(s)	1224
Cac8I	GCNNGC	6 cut(s)	126, 167, 562, 1691, 1695, 1699
CaiI	CAGNNNCTG	1 cut(s)	1270
CfoI	GCGC	1 cut(s)	1262
Cfr10I	RCCGGY	1 cut(s)	1167
Cfr13I	GGNCC	6 cut(s)	49, 57, 122, 573, 845, 1891
ClaI	ATCGAT	1 cut(s)	1730
Csp6I	GTAC	6 cut(s)	29, 278, 1418, 1509, 1590, 2017
CspCI	CAANNNNNGTGG	4 cut(s)	1454, 1489, 1828, 1863
CviAII	CATG	7 cut(s)	178, 623, 764, 1251, 1669, 1867, 1913
CviQI	GTAC	6 cut(s)	29, 278, 1418, 1509, 1590, 2017
DpnI	GATC	9 cut(s)	357, 453, 554, 879, 936, 1134, 1458, 1733, 1974
DpnII	GATC	9 cut(s)	355, 451, 552, 877, 934, 1132, 1456, 1731, 1972
DraI	TTTAAA	1 cut(s)	2005
DraIII	CACNNNGTG	1 cut(s)	383
DrdI	GACNNNNNNGTC	1 cut(s)	889
DseDI	GACNNNNNNGTC	1 cut(s)	889
EaeI	YGGCCR	3 cut(s)	1165, 1298, 1484
Ecl136II	GAGCTC	1 cut(s)	1005
Eco130I	CCWWGG	2 cut(s)	177, 1250
Eco147I	AGGCCT	1 cut(s)	100
Eco24I	GRGCYC	3 cut(s)	38, 1007, 1338
Eco47I	GGWCC	5 cut(s)	49, 57, 573, 845, 1891
Eco53kI	GAGCTC	1 cut(s)	1005
Eco57I	CTGAAG	2 cut(s)	125, 870
EcoICRI	GAGCTC	1 cut(s)	1005
EcoNI	CCTNNNNNAGG	1 cut(s)	1458
EcoO109I	RGGNCCY	3 cut(s)	49, 573, 1891
EcoRI	GAATTC	3 cut(s)	240, 398, 907
EcoRII	CCWGG	6 cut(s)	244, 568, 664, 1329, 1499, 1939
EcoT14I	CCWWGG	2 cut(s)	177, 1250
EcoT38I	GRGCYC	3 cut(s)	38, 1007, 1338
ErhI	CCWWGG	2 cut(s)	177, 1250
FaeI	CATG	7 cut(s)	181, 626, 767, 1254, 1672, 1870, 1916
FaqI	GGGAC	1 cut(s)	559
FatI	CATG	7 cut(s)	177, 622, 763, 1250, 1668, 1866, 1912
FauNDI	CATATG	1 cut(s)	1436
Fnu4HI	GCNGC	5 cut(s)	333, 336, 825, 992, 1767
FokI	GGATG	6 cut(s)	20, 785, 1268, 1288, 1599, 1805
FriOI	GRGCYC	3 cut(s)	38, 1007, 1338
Fsp4HI	GCNGC	5 cut(s)	333, 336, 825, 992, 1767
FspBI	CTAG	6 cut(s)	96, 395, 449, 561, 596, 1851
GlaI	GCGC	1 cut(s)	1261
GluI	GCNGC	5 cut(s)	333, 336, 825, 992, 1767
GsuI	CTGGAG	2 cut(s)	372, 1953
HaeII	RGCGCY	1 cut(s)	1263
HaeIII	GGCC	7 cut(s)	100, 124, 827, 1167, 1300, 1486, 1623
HapII	CCGG	4 cut(s)	120, 1105, 1168, 1661
HhaI	GCGC	1 cut(s)	1262
Hin1II	CATG	7 cut(s)	181, 626, 767, 1254, 1672, 1870, 1916
Hin6I	GCGC	1 cut(s)	1260
HinP1I	GCGC	1 cut(s)	1260
HincII	GTYRAC	3 cut(s)	304, 1653, 1716
HindII	GTYRAC	3 cut(s)	304, 1653, 1716
HinfI	GANTC	6 cut(s)	407, 709, 797, 866, 890, 967
HpaII	CCGG	4 cut(s)	120, 1105, 1168, 1661
HphI	GGTGA	7 cut(s)	395, 416, 938, 1120, 1402, 1443, 1453
Hpy166II	GTNNAC	7 cut(s)	57, 304, 1190, 1393, 1653, 1716, 1903
Hpy188III	TCNNGA	8 cut(s)	455, 596, 728, 1637, 1661, 1682, 1814, 1970
Hpy8I	GTNNAC	7 cut(s)	57, 304, 1190, 1393, 1653, 1716, 1903
HpyAV	CCTTC	8 cut(s)	40, 100, 369, 1126, 1146, 1316, 1577, 1613
HpyCH4III	ACNGT	2 cut(s)	589, 1830
HpyF10VI	GCNNNNNNNGC	4 cut(s)	130, 332, 1011, 1475
Hsp92II	CATG	7 cut(s)	181, 626, 767, 1254, 1672, 1870, 1916
HspAI	GCGC	1 cut(s)	1260
KflI	GGGWCCC	1 cut(s)	573
Kpn2I	TCCGGA	1 cut(s)	1660
Kzo9I	GATC	9 cut(s)	355, 451, 552, 877, 934, 1132, 1456, 1731, 1972
Lsp1109I	GCAGC	4 cut(s)	344, 347, 1003, 1753
LweI	GCATC	5 cut(s)	133, 206, 1072, 1276, 1783
MaeI	CTAG	6 cut(s)	96, 395, 449, 561, 596, 1851
MaeIII	GTNAC	7 cut(s)	109, 383, 404, 544, 606, 1313, 1824
MalI	GATC	9 cut(s)	357, 453, 554, 879, 936, 1134, 1458, 1733, 1974
MbiI	CCGCTC	1 cut(s)	492
MboI	GATC	9 cut(s)	355, 451, 552, 877, 934, 1132, 1456, 1731, 1972
MboII	GAAGA	5 cut(s)	440, 806, 1142, 1274, 1347
MfeI	CAATTG	1 cut(s)	153
MflI	RGATCY	7 cut(s)	355, 451, 552, 877, 934, 1132, 1972
MhlI	GDGCHC	7 cut(s)	38, 516, 1007, 1338, 1668, 1879, 1905
MlsI	TGGCCA	2 cut(s)	1300, 1486
MluNI	TGGCCA	2 cut(s)	1300, 1486
MlyI	GAGTC	2 cut(s)	401, 899
MmeI	TCCRAC	5 cut(s)	235, 905, 1177, 1560, 1783
Mox20I	TGGCCA	2 cut(s)	1300, 1486
MroI	TCCGGA	1 cut(s)	1660
MroXI	GAANNNNTTC	2 cut(s)	971, 1115
MscI	TGGCCA	2 cut(s)	1300, 1486
MseI	TTAA	3 cut(s)	785, 1557, 2004
MslI	CAYNNNNRTG	3 cut(s)	627, 1439, 1911
Msp20I	TGGCCA	2 cut(s)	1300, 1486
MspA1I	CMGCKG	1 cut(s)	338
MspI	CCGG	4 cut(s)	120, 1105, 1168, 1661
MspR9I	CCNGG	8 cut(s)	121, 246, 570, 666, 1106, 1331, 1501, 1941
MunI	CAATTG	1 cut(s)	153
Mva1269I	GAATGC	1 cut(s)	1331
MvaI	CCWGG	6 cut(s)	246, 570, 666, 1331, 1501, 1941
MwoI	GCNNNNNNNGC	4 cut(s)	130, 332, 1011, 1475
NciI	CCSGG	2 cut(s)	121, 1106
NcoI	CCATGG	2 cut(s)	177, 1250
NdeI	CATATG	1 cut(s)	1436
NdeII	GATC	9 cut(s)	355, 451, 552, 877, 934, 1132, 1456, 1731, 1972
NheI	GCTAGC	1 cut(s)	560
NlaIII	CATG	7 cut(s)	181, 626, 767, 1254, 1672, 1870, 1916
NmuCI	GTSAC	5 cut(s)	109, 383, 404, 544, 1824
NspI	RCATGY	4 cut(s)	626, 767, 1672, 1916
PceI	AGGCCT	1 cut(s)	100
PciI	ACATGT	4 cut(s)	622, 763, 1668, 1912
PctI	GAATGC	1 cut(s)	1331
PdmI	GAANNNNTTC	2 cut(s)	971, 1115
PfeI	GAWTC	4 cut(s)	709, 797, 866, 967
PflMI	CCANNNNNTGG	3 cut(s)	183, 1256, 1410
PfoI	TCCNGGA	1 cut(s)	1939
PkrI	GCNGC	5 cut(s)	334, 337, 826, 993, 1768
PleI	GAGTC	2 cut(s)	401, 898
PpsI	GAGTC	2 cut(s)	401, 898
PpuMI	RGGWCCY	3 cut(s)	49, 573, 1891
PscI	ACATGT	4 cut(s)	622, 763, 1668, 1912
Psp124BI	GAGCTC	1 cut(s)	1007
Psp5II	RGGWCCY	3 cut(s)	49, 573, 1891
Psp6I	CCWGG	6 cut(s)	244, 568, 664, 1329, 1499, 1939
PspGI	CCWGG	6 cut(s)	244, 568, 664, 1329, 1499, 1939
PspPI	GGNCC	6 cut(s)	49, 57, 122, 573, 845, 1891
PspPPI	RGGWCCY	3 cut(s)	49, 573, 1891
PstI	CTGCAG	3 cut(s)	43, 334, 1221
PstNI	CAGNNNCTG	1 cut(s)	1270
PsuI	RGATCY	7 cut(s)	355, 451, 552, 877, 934, 1132, 1972
RsaI	GTAC	6 cut(s)	30, 279, 1419, 1510, 1591, 2018
RsaNI	GTAC	6 cut(s)	29, 278, 1418, 1509, 1590, 2017
RseI	CAYNNNNRTG	3 cut(s)	627, 1439, 1911
SacI	GAGCTC	1 cut(s)	1007
SaqAI	TTAA	3 cut(s)	785, 1557, 2004
SatI	GCNGC	5 cut(s)	333, 336, 825, 992, 1767
Sau3AI	GATC	9 cut(s)	355, 451, 552, 877, 934, 1132, 1456, 1731, 1972
Sau96I	GGNCC	6 cut(s)	49, 57, 122, 573, 845, 1891
SbfI	CCTGCAGG	1 cut(s)	1221
SchI	GAGTC	2 cut(s)	401, 899
ScrFI	CCNGG	8 cut(s)	121, 246, 570, 666, 1106, 1331, 1501, 1941
SdaI	CCTGCAGG	1 cut(s)	1221
SduI	GDGCHC	7 cut(s)	38, 516, 1007, 1338, 1668, 1879, 1905
SfaNI	GCATC	5 cut(s)	133, 206, 1072, 1276, 1783
SfcI	CTRYAG	3 cut(s)	39, 330, 1217
SinI	GGWCC	5 cut(s)	49, 57, 573, 845, 1891
SmiMI	CAYNNNNRTG	3 cut(s)	627, 1439, 1911
SmlI	CTYRAG	1 cut(s)	1203
SmoI	CTYRAG	1 cut(s)	1203
Sse8387I	CCTGCAGG	1 cut(s)	1221
SseBI	AGGCCT	1 cut(s)	100
SsiI	CCGC	5 cut(s)	338, 492, 558, 824, 1985
SspMI	CTAG	6 cut(s)	96, 395, 449, 561, 596, 1851
SstI	GAGCTC	1 cut(s)	1007
StuI	AGGCCT	1 cut(s)	100
StyD4I	CCNGG	8 cut(s)	119, 244, 568, 664, 1104, 1329, 1499, 1939
StyI	CCWWGG	2 cut(s)	177, 1250
TaaI	ACNGT	2 cut(s)	589, 1830
TaqI	TCGA	4 cut(s)	321, 1459, 1730, 1909
TatI	WGTACW	4 cut(s)	1417, 1508, 1589, 2016
TauI	GCSGC	1 cut(s)	827
TfiI	GAWTC	4 cut(s)	709, 797, 866, 967
Tru1I	TTAA	3 cut(s)	785, 1557, 2004
Tru9I	TTAA	3 cut(s)	785, 1557, 2004
TscAI	CASTG	5 cut(s)	140, 492, 757, 904, 1411
TseFI	GTSAC	5 cut(s)	109, 383, 404, 544, 1824
TseI	GCWGC	4 cut(s)	332, 335, 991, 1766
Tsp45I	GTSAC	5 cut(s)	109, 383, 404, 544, 1824
TspDTI	ATGAA	6 cut(s)	17, 441, 510, 912, 1143, 1304
TspGWI	ACGGA	1 cut(s)	46
TspRI	CASTG	5 cut(s)	140, 492, 757, 904, 1411
Van91I	CCANNNNNTGG	3 cut(s)	183, 1256, 1410
VneI	GTGCAC	1 cut(s)	1901
VpaK11BI	GGWCC	5 cut(s)	49, 57, 573, 845, 1891
XagI	CCTNNNNNAGG	1 cut(s)	1458
XapI	RAATTY	4 cut(s)	240, 398, 907, 919
XbaI	TCTAGA	1 cut(s)	595
XceI	RCATGY	4 cut(s)	626, 767, 1672, 1916
XmnI	GAANNNNTTC	2 cut(s)	971, 1115
XspI	CTAG	6 cut(s)	96, 395, 449, 561, 596, 1851

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.