Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr6D

Orthogroup ID

OG0004443

Physical Location & Seq

Genomic Coordinates Reverse (-)

6106641 .. 6113342

Gene Structure 6702 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh6DG059600.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2085 bp

ATGGAGGATGGTGACTGGGTTCTGGTGCGGCCGCCTGCAGAGAAGGACCTCTGGACCCCAAGTTTAGTGAAGGAGGATGACAGTTCTAGGCCTTTGAAGGTTACTTTTGGTGGGCCTGCGAAGTTCTGGACTGATGCAATTCCAATTGGGAATGGGAGGCTTGGAGCTATGGTCTGGGGTGGTGTGGCTTCTGAAACTCTTCAGCTCAATGAGGACACACTCTGGACTGGAACTCCAGGCAACTACACCAACCCAAATGCTCCACAGGCGCTAACAGAGGTTAGGAAACTTGTTGATGATGGTAACTATGTTGAAGCTACTGAAGCAGCGGTCAAGTTGACTGGAGATCCTTCTGATGTATACCAACTACTCGGCGACATCAACCTAGAATTTGATGATTCGCATCTCAAGTATGCTGAAGAAACTTACAGCAGAGAGCTAGACCTAGATACTGCAACAGCAAGGATAAAATATTCTGTGGGGGATGTAGAATATACTAGGGAGCATTTTGCTTCGAACCCCGATCAAGTGATTGTGACAAAGATCTCTGCAAGCAAATCAGGGTCCCTATCATTTACAGTTTCTCTAGACAGCAAGATGCATCATGGTTCACATCTAAATGGTAAAAACCAAATTATACTTGAAGGAAGTTGTCCTGGTAAAAGAATTCCACCAAAGTATAATGAAATTCCAAAGGGGATTCAGTTTACTGCAGTTCTTGATTTGCAGATTAGTGGTGACAAAGGTGTGATACATGCTTTGGATGACAAGAAATTTAGAGTTGAAAAAGCAGATTGGGCTGTTTTGCTTCTGGTGGCCTCATCTTCATTTAAAGATCCATTTACTAAGCCCTCTGATTCTAAGAGAAATCCAACTTCAGAGTCTCTCACTGCATTGAATTCAATAAGAAATTTGTCATATTCTGACCTCTATGCTCATCATTTGGATGACTATCAGAATCTTTTTCATCGTGTCTCATTGCAGCTCTCTAAGAGCTCTAAGAGCATATTAGGAAATAAAACTTTGGAGATGAAGAAGCTCAACCCTATTACTAATTTGAATTTCAAGGGAAGTGACAATGCCTTGGGTTCTACTGCTGACAGGGTGAAATCCTTTAAAACTGATGAAGATCCTTCTTTTGTGGAACTCTTGTTCCAATATGGTCGATATCTTCTTATTTCATGCTCACGGCCTGGAACTCAGCCGGCAAACCTGCAGGGTATATGGAACTACAAAATTGAACCTGCATGGGATGGTGCTCCTCATACGAACATTAATCTTCAGATGAACTATTGGCCTTCCCTTCCTTGTAATCTACGAGAATGCCAGGAACCCTTATTTGATTACACATCTTTATTATCTATTAACGGAAGTAAAACAGCAAAGGTCAACTACGAAGCTAGTGGTTGGGTTGTTCATCAAGTGACTGACATATGGGCGAAAACATCACCAGATCGAGGTCAAGCTGTATGGGCTTTATGGCCAATGGGAGGAGCGTGGATCTGTACTCATCTATGGGAACATTATACTTATACAATGGACAAAGATTTCCTGAAAAATAAGGCATATCCTTTGTTGGAAGGATGTGCATCATTTCTACTGGACTGGTTGATTGAAGGACGTGGCGGGTATCTTGAAACCAACCCATCAACTTCTCCAGAGCACATGTTTATTGCTCCTGATGGTAAACAGGCCAGTGTGAGCTACTCATCAACCATGGACATCTCAATCATAAAAGAAGTATTTTCTGCAATACTTTCTGCTGCTGAGGTTTTAGGAAAAACTCAAGATGCTTTTATTGAAAAAGTTCGCAATGCTCAATCTAGGCTTCCACCCACAAAAATTGCTAGAGATGGTTCTATTATGGAATGGGCACAAGATTTTGAGGACCCAGAAGTGCACCATCGACATGTATCACACCTGTTTGGCCTCTTTCCCGGTCACACAATAACTCTTGAAAAAAATCCAGACCTCTGTAAAGCAGTGGATTATACCCTTTTTAAAAGAGGTTTGTATGTCACAAGTAAATGTATCATTTTGGTCTTGCAATCCCCAGGAAGTGACTTCCTTTTAAGAAATAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004553 GO:0004560 GO:0005575 GO:0005576 GO:0015928 GO:0016787 GO:0016798 GO:0047513 GO:0048046

KEGG Pathways

Metabolic & Signaling

ko00511 map00511

Pfam Domains

Protein Families

Glyco_hyd_65N_2

Protein Analysis

694

Amino Acids

77.45

Weight (kDa)

5.73

Isoelectric Point (pI)

31.76

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hyd_65N_2	PF14498	36 - 278	6.2e-64	Glycosyl hydrolase family 65, N-terminal domain
Glyco_hydro_95_cat	PF22124	306 - 670	8.6e-138	Glycosyl hydrolase family 95 catalytic domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004443)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G34260
fragaria_vesca	FvH4_2g04810 FvH4_2g04810 FvH4_2g04810 FvH4_2g04810 FvH4_2g04870 FvH4_2g04870 FvH4_2g04870 FvH4_2g04870
malus_domestica	MD10G1099200.v1.1 MD10G1099400.v1.1
prunus_persica	Prupe.8G137300_v2.0.a1 Prupe.8G137300_v2.0.a1 Prupe.8G137300_v2.0.a1
pyrus_communis	pycom10g08550 pycom10g08560 pycom10g08570
rosa_chinensis	RchiOBHm_Chr6g0254541 RchiOBHm_Chr6g0254581
rosa_laevigata	RLG00000014903 RLG00000014905
rosa_multiflora	Rmu_co8517753.1_g000001 Rmu_sc0008789.1_g000011 Rmu_sc0009578.1_g000012
rosa_roxburghii	Rroxscaffold_7G00210690
rosa_rugosa	Rorug05G0553300
rosa_samantha	Rh6AG070200 Rh6BG062700 Rh6CG062600 Rh6DG059600
rosa_wichuraiana	Rw6G006150 Rw6G006190

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	1221, 1252
AccI	GTMKAC	1 cut(s)	360
AciI	CCGC	4 cut(s)	28, 32, 329, 1626
AclWI	GGATC	4 cut(s)	341, 830, 1124, 1508
AcoI	YGGCCR	2 cut(s)	29, 1481
AcsI	RAATTY	7 cut(s)	389, 666, 687, 773, 898, 910, 1060
AcuI	CTGAAG	5 cut(s)	185, 342, 438, 861, 1265
AfaI	GTAC	1 cut(s)	1507
AfiI	CCNNNNNNNGG	2 cut(s)	1457, 1490
AflIII	ACRYGT	2 cut(s)	1665, 1909
AjiI	CACGTC	1 cut(s)	1622
AjnI	CCWGG	5 cut(s)	235, 655, 1192, 1326, 2053
AloI	GAACNNNNNNTCC	4 cut(s)	1136, 1137, 1168, 1169
Alw21I	GWGCWC	4 cut(s)	998, 1261, 1665, 1902
Alw26I	GTCTC	2 cut(s)	888, 979
Alw44I	GTGCAC	1 cut(s)	1898
AlwI	GGATC	4 cut(s)	341, 830, 1124, 1508
AoxI	GGCC	9 cut(s)	29, 89, 113, 816, 1190, 1295, 1481, 1692, 1927
ApaLI	GTGCAC	1 cut(s)	1898
ApeKI	GCWGC	3 cut(s)	326, 982, 1763
ApoI	RAATTY	7 cut(s)	389, 666, 687, 773, 898, 910, 1060
AseI	ATTAAT	1 cut(s)	1275
Asp700I	GAANNNNTTC	2 cut(s)	198, 1806
AspLEI	GCGC	1 cut(s)	271
AspS9I	GGNCC	5 cut(s)	46, 54, 113, 564, 1888
AsuC2I	CCSGG	1 cut(s)	1938
AsuHPI	GGTGA	4 cut(s)	23, 749, 1117, 1440
AsuII	TTCGAA	1 cut(s)	515
AvaII	GGWCC	4 cut(s)	46, 54, 564, 1888
BaeGI	GKGCMC	2 cut(s)	1876, 1902
BalI	TGGCCA	1 cut(s)	1483
BanII	GRGCYC	1 cut(s)	998
Bbv12I	GWGCWC	4 cut(s)	998, 1261, 1665, 1902
BbvCI	CCTCAGC	1 cut(s)	1767
BbvI	GCAGC	3 cut(s)	338, 994, 1750
BccI	CCATC	7 cut(s)	2, 293, 1247, 1654, 1676, 1847, 1911
BceAI	ACGGC	1 cut(s)	1205
BciT130I	CCWGG	5 cut(s)	237, 657, 1194, 1328, 2055
BcnI	CCSGG	1 cut(s)	1938
BcoDI	GTCTC	2 cut(s)	888, 979
BfaI	CTAG	9 cut(s)	87, 386, 440, 446, 498, 587, 1401, 1824, 1848
BfmI	CTRYAG	3 cut(s)	36, 711, 1214
BfoI	RGCGCY	1 cut(s)	272
BfuAI	ACCTGC	2 cut(s)	1221, 1252
BglII	AGATCT	1 cut(s)	543
BisI	GCNGC	5 cut(s)	29, 32, 327, 983, 1764
BlsI	GCNGC	5 cut(s)	30, 33, 328, 984, 1765
Bme1390I	CCNGG	6 cut(s)	237, 657, 1194, 1328, 1938, 2055
Bme18I	GGWCC	4 cut(s)	46, 54, 564, 1888
BmgBI	CACGTC	1 cut(s)	1622
BmgT120I	GGNCC	5 cut(s)	46, 54, 113, 564, 1888
BmiI	GGNNCC	5 cut(s)	56, 565, 566, 1332, 1890
BmrFI	CCNGG	6 cut(s)	237, 657, 1194, 1328, 1938, 2055
BmrI	ACTGGG	1 cut(s)	25
BmsI	GCATC	6 cut(s)	124, 412, 588, 610, 1598, 1780
BmuI	ACTGGG	1 cut(s)	25
BpmI	CTGGAG	3 cut(s)	219, 363, 1641
Bpu10I	CCTNAGC	1 cut(s)	1767
Bpu14I	TTCGAA	1 cut(s)	515
BpuEI	CTTGAG	2 cut(s)	392, 1770
BpuMI	CCSGG	1 cut(s)	1938
BsaBI	GATNNNNATC	4 cut(s)	402, 951, 1128, 1588
BsaJI	CCNNGG	3 cut(s)	1083, 1716, 2053
BsaXI	ACNNNNNCTCC	4 cut(s)	156, 186, 217, 247
Bsc4I	CCNNNNNNNGG	2 cut(s)	1457, 1490
Bse118I	RCCGGY	1 cut(s)	1204
Bse1I	ACTGG	6 cut(s)	20, 232, 346, 1605, 1610, 1695
Bse3DI	GCAATG	2 cut(s)	977, 1819
Bse8I	GATNNNNATC	4 cut(s)	402, 951, 1128, 1588
BseBI	CCWGG	5 cut(s)	237, 657, 1194, 1328, 2055
BseDI	CCNNGG	3 cut(s)	1083, 1716, 2053
BseGI	GGATG	7 cut(s)	13, 82, 490, 769, 952, 1258, 1589
BseJI	GATNNNNATC	4 cut(s)	402, 951, 1128, 1588
BseLI	CCNNNNNNNGG	2 cut(s)	1457, 1490
BseMI	GCAATG	2 cut(s)	977, 1819
BseMII	CTCAG	2 cut(s)	1214, 1758
BseNI	ACTGG	6 cut(s)	20, 232, 346, 1605, 1610, 1695
BseRI	GAGGAG	2 cut(s)	1251, 1506
BseSI	GKGCMC	2 cut(s)	1876, 1902
BseX3I	CGGCCG	1 cut(s)	29
BseXI	GCAGC	3 cut(s)	338, 994, 1750
Bsh1285I	CGRYCG	1 cut(s)	32
BshFI	GGCC	9 cut(s)	31, 91, 115, 818, 1192, 1297, 1483, 1694, 1929
BsiEI	CGRYCG	1 cut(s)	32
BsiHKAI	GWGCWC	4 cut(s)	998, 1261, 1665, 1902
BsiSI	CCGG	2 cut(s)	1205, 1938
BslFI	GGGAC	1 cut(s)	550
BslI	CCNNNNNNNGG	2 cut(s)	1457, 1490
BsmAI	GTCTC	2 cut(s)	888, 979
BsmFI	GGGAC	1 cut(s)	550
BsmI	GAATGC	1 cut(s)	1328
BsnI	GGCC	9 cut(s)	31, 91, 115, 818, 1192, 1297, 1483, 1694, 1929
Bsp119I	TTCGAA	1 cut(s)	515
Bsp1286I	GDGCHC	5 cut(s)	998, 1261, 1665, 1876, 1902
Bsp143I	GATC	7 cut(s)	346, 523, 543, 835, 1129, 1453, 1500
Bsp19I	CCATGG	1 cut(s)	1716
BspACI	CCGC	4 cut(s)	28, 32, 329, 1626
BspANI	GGCC	9 cut(s)	31, 91, 115, 818, 1192, 1297, 1483, 1694, 1929
BspCNI	CTCAG	2 cut(s)	1213, 1759
BspLI	GGNNCC	5 cut(s)	56, 565, 566, 1332, 1890
BspMAI	CTGCAG	3 cut(s)	40, 715, 1218
BspMI	ACCTGC	2 cut(s)	1221, 1252
BspPI	GGATC	4 cut(s)	341, 830, 1124, 1508
BspT104I	TTCGAA	1 cut(s)	515
BsrDI	GCAATG	2 cut(s)	977, 1819
BsrFI	RCCGGY	1 cut(s)	1204
BsrI	ACTGG	6 cut(s)	20, 232, 346, 1605, 1610, 1695
BssAI	RCCGGY	1 cut(s)	1204
BssECI	CCNNGG	3 cut(s)	1083, 1716, 2053
BssMI	GATC	7 cut(s)	346, 523, 543, 835, 1129, 1453, 1500
BssNAI	GTATAC	1 cut(s)	361
BssT1I	CCWWGG	2 cut(s)	1083, 1716
Bst1107I	GTATAC	1 cut(s)	361
Bst2UI	CCWGG	5 cut(s)	237, 657, 1194, 1328, 2055
Bst4CI	ACNGT	2 cut(s)	83, 580
Bst6I	CTCTTC	1 cut(s)	204
BstBI	TTCGAA	1 cut(s)	515
BstC8I	GCNNGC	4 cut(s)	36, 117, 553, 1206
BstDEI	CTNAG	6 cut(s)	846, 861, 990, 999, 1200, 1767
BstDSI	CCRYGG	1 cut(s)	1716
BstF5I	GGATG	7 cut(s)	13, 82, 490, 769, 952, 1258, 1589
BstH2I	RGCGCY	1 cut(s)	272
BstHHI	GCGC	1 cut(s)	271
BstKTI	GATC	7 cut(s)	349, 526, 546, 838, 1132, 1456, 1503
BstMAI	GTCTC	2 cut(s)	888, 979
BstMBI	GATC	7 cut(s)	346, 523, 543, 835, 1129, 1453, 1500
BstMCI	CGRYCG	1 cut(s)	32
BstMWI	GCNNNNNNNGC	5 cut(s)	266, 323, 797, 1002, 1472
BstNI	CCWGG	5 cut(s)	237, 657, 1194, 1328, 2055
BstNSI	RCATGY	3 cut(s)	758, 1669, 1913
BstSCI	CCNGG	6 cut(s)	235, 655, 1192, 1326, 1936, 2053
BstSFI	CTRYAG	3 cut(s)	36, 711, 1214
BstSLI	GKGCMC	2 cut(s)	1876, 1902
BstV1I	GCAGC	3 cut(s)	338, 994, 1750
BstX2I	RGATCY	5 cut(s)	346, 543, 835, 1129, 1500
BstYI	RGATCY	5 cut(s)	346, 543, 835, 1129, 1500
BstZ17I	GTATAC	1 cut(s)	361
BstZI	CGGCCG	1 cut(s)	29
BsuRI	GGCC	9 cut(s)	31, 91, 115, 818, 1192, 1297, 1483, 1694, 1929
BtgI	CCRYGG	1 cut(s)	1716
BtrI	CACGTC	1 cut(s)	1622
BtsCI	GGATG	7 cut(s)	13, 82, 490, 769, 952, 1258, 1589
BtsI	GCAGTG	2 cut(s)	888, 1989
BtsIMutI	CAGTG	3 cut(s)	888, 1702, 1989
BveI	ACCTGC	2 cut(s)	1221, 1252
Cac8I	GCNNGC	4 cut(s)	36, 117, 553, 1206
CciNI	GCGGCCGC	1 cut(s)	29
CfoI	GCGC	1 cut(s)	271
Cfr10I	RCCGGY	1 cut(s)	1204
Cfr13I	GGNCC	5 cut(s)	46, 54, 113, 564, 1888
Csp6I	GTAC	1 cut(s)	1506
CspCI	CAANNNNNGTGG	2 cut(s)	1825, 1860
CviAII	CATG	7 cut(s)	605, 755, 1182, 1248, 1666, 1717, 1910
CviQI	GTAC	1 cut(s)	1506
DdeI	CTNAG	6 cut(s)	846, 861, 990, 999, 1200, 1767
DpnI	GATC	7 cut(s)	348, 525, 545, 837, 1131, 1455, 1502
DpnII	GATC	7 cut(s)	346, 523, 543, 835, 1129, 1453, 1500
DraI	TTTAAA	3 cut(s)	832, 1117, 2002
EaeI	YGGCCR	2 cut(s)	29, 1481
EagI	CGGCCG	1 cut(s)	29
Eam1104I	CTCTTC	1 cut(s)	204
EarI	CTCTTC	1 cut(s)	204
Ecl136II	GAGCTC	1 cut(s)	996
EclXI	CGGCCG	1 cut(s)	29
Eco130I	CCWWGG	2 cut(s)	1083, 1716
Eco147I	AGGCCT	1 cut(s)	91
Eco24I	GRGCYC	1 cut(s)	998
Eco32I	GATATC	1 cut(s)	1169
Eco47I	GGWCC	4 cut(s)	46, 54, 564, 1888
Eco52I	CGGCCG	1 cut(s)	29
Eco53kI	GAGCTC	1 cut(s)	996
Eco57I	CTGAAG	5 cut(s)	185, 342, 438, 861, 1265
EcoICRI	GAGCTC	1 cut(s)	996
EcoO109I	RGGNCCY	3 cut(s)	46, 564, 1888
EcoRI	GAATTC	2 cut(s)	666, 898
EcoRII	CCWGG	5 cut(s)	235, 655, 1192, 1326, 2053
EcoRV	GATATC	1 cut(s)	1169
EcoT14I	CCWWGG	2 cut(s)	1083, 1716
EcoT22I	ATGCAT	1 cut(s)	603
EcoT38I	GRGCYC	1 cut(s)	998
ErhI	CCWWGG	2 cut(s)	1083, 1716
FaeI	CATG	7 cut(s)	608, 758, 1185, 1251, 1669, 1720, 1913
FaqI	GGGAC	1 cut(s)	550
FatI	CATG	7 cut(s)	604, 754, 1181, 1247, 1665, 1716, 1909
FauI	CCCGC	1 cut(s)	1619
FauNDI	CATATG	1 cut(s)	1433
FblI	GTMKAC	1 cut(s)	360
Fnu4HI	GCNGC	5 cut(s)	29, 32, 327, 983, 1764
FokI	GGATG	7 cut(s)	20, 89, 497, 776, 959, 1265, 1596
FriOI	GRGCYC	1 cut(s)	998
Fsp4HI	GCNGC	5 cut(s)	29, 32, 327, 983, 1764
FspBI	CTAG	9 cut(s)	87, 386, 440, 446, 498, 587, 1401, 1824, 1848
GlaI	GCGC	1 cut(s)	270
GluI	GCNGC	5 cut(s)	29, 32, 327, 983, 1764
GsuI	CTGGAG	3 cut(s)	219, 363, 1641
HaeII	RGCGCY	1 cut(s)	272
HaeIII	GGCC	9 cut(s)	31, 91, 115, 818, 1192, 1297, 1483, 1694, 1929
HapII	CCGG	2 cut(s)	1205, 1938
HhaI	GCGC	1 cut(s)	271
Hin1II	CATG	7 cut(s)	608, 758, 1185, 1251, 1669, 1720, 1913
Hin6I	GCGC	1 cut(s)	269
HinP1I	GCGC	1 cut(s)	269
HincII	GTYRAC	2 cut(s)	339, 1390
HindII	GTYRAC	2 cut(s)	339, 1390
HinfI	GANTC	5 cut(s)	398, 700, 857, 881, 958
HpaII	CCGG	2 cut(s)	1205, 1938
HphI	GGTGA	4 cut(s)	23, 749, 1117, 1440
Hpy166II	GTNNAC	7 cut(s)	339, 361, 611, 708, 1390, 1688, 1900
Hpy188I	TCNGA	7 cut(s)	193, 355, 856, 880, 925, 957, 1284
Hpy8I	GTNNAC	7 cut(s)	339, 361, 611, 708, 1390, 1688, 1900
HpyCH4III	ACNGT	2 cut(s)	83, 580
HpyCH4IV	ACGT	1 cut(s)	1621
HpyF10VI	GCNNNNNNNGC	5 cut(s)	266, 323, 797, 1002, 1472
HpyF3I	CTNAG	6 cut(s)	846, 861, 990, 999, 1200, 1767
HpySE526I	ACGT	1 cut(s)	1621
Hsp92II	CATG	7 cut(s)	608, 758, 1185, 1251, 1669, 1720, 1913
HspAI	GCGC	1 cut(s)	269
KflI	GGGWCCC	1 cut(s)	564
KroI	GCCGGC	1 cut(s)	1204
KroNI	GCCGGC	1 cut(s)	1206
Kzo9I	GATC	7 cut(s)	346, 523, 543, 835, 1129, 1453, 1500
LmnI	GCTCC	6 cut(s)	164, 265, 502, 1264, 1493, 1681
Lsp1109I	GCAGC	3 cut(s)	338, 994, 1750
LweI	GCATC	6 cut(s)	124, 412, 588, 610, 1598, 1780
MaeI	CTAG	9 cut(s)	87, 386, 440, 446, 498, 587, 1401, 1824, 1848
MaeII	ACGT	1 cut(s)	1621
MalI	GATC	7 cut(s)	348, 525, 545, 837, 1131, 1455, 1502
MboI	GATC	7 cut(s)	346, 523, 543, 835, 1129, 1453, 1500
MboII	GAAGA	7 cut(s)	191, 431, 816, 1045, 1139, 1163, 1271
MfeI	CAATTG	1 cut(s)	144
MflI	RGATCY	5 cut(s)	346, 543, 835, 1129, 1500
MhlI	GDGCHC	5 cut(s)	998, 1261, 1665, 1876, 1902
MlsI	TGGCCA	1 cut(s)	1483
MluNI	TGGCCA	1 cut(s)	1483
MlyI	GAGTC	1 cut(s)	890
MmeI	TCCRAC	2 cut(s)	896, 1557
Mox20I	TGGCCA	1 cut(s)	1483
Mph1103I	ATGCAT	1 cut(s)	603
MroNI	GCCGGC	1 cut(s)	1204
MroXI	GAANNNNTTC	2 cut(s)	198, 1806
MscI	TGGCCA	1 cut(s)	1483
MseI	TTAA	6 cut(s)	831, 1116, 1275, 1365, 2001, 2072
MslI	CAYNNNNRTG	3 cut(s)	618, 945, 1908
Msp20I	TGGCCA	1 cut(s)	1483
MspA1I	CMGCKG	1 cut(s)	329
MspI	CCGG	2 cut(s)	1205, 1938
MspR9I	CCNGG	6 cut(s)	237, 657, 1194, 1328, 1938, 2055
MunI	CAATTG	1 cut(s)	144
Mva1269I	GAATGC	1 cut(s)	1328
MvaI	CCWGG	5 cut(s)	237, 657, 1194, 1328, 2055
MwoI	GCNNNNNNNGC	5 cut(s)	266, 323, 797, 1002, 1472
NaeI	GCCGGC	1 cut(s)	1206
NciI	CCSGG	1 cut(s)	1938
NcoI	CCATGG	1 cut(s)	1716
NdeI	CATATG	1 cut(s)	1433
NdeII	GATC	7 cut(s)	346, 523, 543, 835, 1129, 1453, 1500
NgoMIV	GCCGGC	1 cut(s)	1204
NlaIII	CATG	7 cut(s)	608, 758, 1185, 1251, 1669, 1720, 1913
NlaIV	GGNNCC	5 cut(s)	56, 565, 566, 1332, 1890
NmeAIII	GCCGAG	1 cut(s)	351
NmuCI	GTSAC	8 cut(s)	11, 535, 737, 1073, 1423, 1940, 2017, 2060
NotI	GCGGCCGC	1 cut(s)	29
NsiI	ATGCAT	1 cut(s)	603
NspI	RCATGY	3 cut(s)	758, 1669, 1913
NspV	TTCGAA	1 cut(s)	515
PceI	AGGCCT	1 cut(s)	91
PciI	ACATGT	2 cut(s)	1665, 1909
PctI	GAATGC	1 cut(s)	1328
PdiI	GCCGGC	1 cut(s)	1206
PdmI	GAANNNNTTC	2 cut(s)	198, 1806
PfeI	GAWTC	4 cut(s)	398, 700, 857, 958
PkrI	GCNGC	5 cut(s)	30, 33, 328, 984, 1765
PleI	GAGTC	1 cut(s)	889
PpsI	GAGTC	1 cut(s)	889
PpuMI	RGGWCCY	3 cut(s)	46, 564, 1888
PscI	ACATGT	2 cut(s)	1665, 1909
PshBI	ATTAAT	1 cut(s)	1275
Psp124BI	GAGCTC	1 cut(s)	998
Psp5II	RGGWCCY	3 cut(s)	46, 564, 1888
Psp6I	CCWGG	5 cut(s)	235, 655, 1192, 1326, 2053
PspGI	CCWGG	5 cut(s)	235, 655, 1192, 1326, 2053
PspN4I	GGNNCC	5 cut(s)	56, 565, 566, 1332, 1890
PspPI	GGNCC	5 cut(s)	46, 54, 113, 564, 1888
PspPPI	RGGWCCY	3 cut(s)	46, 564, 1888
PstI	CTGCAG	3 cut(s)	40, 715, 1218
PsuI	RGATCY	5 cut(s)	346, 543, 835, 1129, 1500
RsaI	GTAC	1 cut(s)	1507
RsaNI	GTAC	1 cut(s)	1506
RseI	CAYNNNNRTG	3 cut(s)	618, 945, 1908
SacI	GAGCTC	1 cut(s)	998
SaqAI	TTAA	6 cut(s)	831, 1116, 1275, 1365, 2001, 2072
SatI	GCNGC	5 cut(s)	29, 32, 327, 983, 1764
Sau3AI	GATC	7 cut(s)	346, 523, 543, 835, 1129, 1453, 1500
Sau96I	GGNCC	5 cut(s)	46, 54, 113, 564, 1888
SbfI	CCTGCAGG	1 cut(s)	1218
SchI	GAGTC	1 cut(s)	890
ScrFI	CCNGG	6 cut(s)	237, 657, 1194, 1328, 1938, 2055
SdaI	CCTGCAGG	1 cut(s)	1218
SduI	GDGCHC	5 cut(s)	998, 1261, 1665, 1876, 1902
SfaNI	GCATC	6 cut(s)	124, 412, 588, 610, 1598, 1780
SfcI	CTRYAG	3 cut(s)	36, 711, 1214
SfuI	TTCGAA	1 cut(s)	515
SinI	GGWCC	4 cut(s)	46, 54, 564, 1888
SmiMI	CAYNNNNRTG	3 cut(s)	618, 945, 1908
SmlI	CTYRAG	2 cut(s)	407, 1785
SmoI	CTYRAG	2 cut(s)	407, 1785
Sse8387I	CCTGCAGG	1 cut(s)	1218
SseBI	AGGCCT	1 cut(s)	91
SsiI	CCGC	4 cut(s)	28, 32, 329, 1626
SspI	AATATT	1 cut(s)	473
SspMI	CTAG	9 cut(s)	87, 386, 440, 446, 498, 587, 1401, 1824, 1848
SstI	GAGCTC	1 cut(s)	998
StuI	AGGCCT	1 cut(s)	91
StyD4I	CCNGG	6 cut(s)	235, 655, 1192, 1326, 1936, 2053
StyI	CCWWGG	2 cut(s)	1083, 1716
TaaI	ACNGT	2 cut(s)	83, 580
TaiI	ACGT	1 cut(s)	1624
TaqI	TCGA	4 cut(s)	515, 1165, 1456, 1906
TatI	WGTACW	1 cut(s)	1505
TauI	GCSGC	2 cut(s)	31, 34
TfiI	GAWTC	4 cut(s)	398, 700, 857, 958
Tru1I	TTAA	6 cut(s)	831, 1116, 1275, 1365, 2001, 2072
Tru9I	TTAA	6 cut(s)	831, 1116, 1275, 1365, 2001, 2072
TscAI	CASTG	3 cut(s)	895, 1702, 1989
TseFI	GTSAC	8 cut(s)	11, 535, 737, 1073, 1423, 1940, 2017, 2060
TseI	GCWGC	3 cut(s)	326, 982, 1763
Tsp45I	GTSAC	8 cut(s)	11, 535, 737, 1073, 1423, 1940, 2017, 2060
TspDTI	ATGAA	8 cut(s)	699, 816, 956, 1046, 1140, 1170, 1301, 1406
TspGWI	ACGGA	1 cut(s)	1383
TspRI	CASTG	3 cut(s)	895, 1702, 1989
VneI	GTGCAC	1 cut(s)	1898
VpaK11BI	GGWCC	4 cut(s)	46, 54, 564, 1888
VspI	ATTAAT	1 cut(s)	1275
XapI	RAATTY	7 cut(s)	389, 666, 687, 773, 898, 910, 1060
XbaI	TCTAGA	1 cut(s)	586
XceI	RCATGY	3 cut(s)	758, 1669, 1913
XmiI	GTMKAC	1 cut(s)	360
XmnI	GAANNNNTTC	2 cut(s)	198, 1806
XspI	CTAG	9 cut(s)	87, 386, 440, 446, 498, 587, 1401, 1824, 1848
Zsp2I	ATGCAT	1 cut(s)	603

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.