RLG00000014903

alpha-L-fucosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0004443
Physical Location & Seq
Forward (+)
59319852 .. 59322052
2201 bp
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UTR
Exon/CDS
Intron
RLM00000014903

Sequence Viewer

Length: 588 bp
ATGACTGGTTTTCATGGGACCCTTTTCTTTGCTTCACCAGACAGAACCCATCTCACCTCTACTCCTCAATACTCACTACTCACCGAACCCAAAAACCCCACAACCCTTAATTTCTCTGCCTCACTCTCATTCACACTCTTTCACACTCTGTCTGCACCGACTAACAGCTCATTTGTGTCACTGAAAGATGGTGACTGGGTTCTGGTGCGGCCGCCTGCAGAGAAGGACCTCTGGACTCCGAGTTTAGTGAAGGAGGATGATAGTTCTAAGCCTTTGAAGGTTACTTTTGGTGGGCCTGTGAAGTTCTGGACTGATGCAATTCCAATTGGGAATGGGAGGCTTGGAGCTATGGTCTGGGGTGGTGTGGCTTCAGAAACTCTTCAGCTCAATGAAGACACACTCTGGACTGGAACTCCGGGCAACTACACCAACCCAAATGCTCCACAGGCACTAACAGAGGTTAGGAAACTTGTTGATGACGAGTTGTCCGGAGATCCTTTTGATGTATGTAACTTCATCCGCTTCGATTATGCTTCCTTCGTCATTTTGCTACTCAGTAGAATTGAACTTGTACTGAATATTGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004553 GO:0004560 GO:0005575 GO:0005576 GO:0015928 GO:0016787 GO:0016798 GO:0047513 GO:0048046
KEGG Pathways
Metabolic & Signaling
ko00511 map00511
Pfam Domains
Protein Families
Glyco_hyd_65N_2

Protein Analysis

196

Amino Acids

21.47

Weight (kDa)

4.77

Isoelectric Point (pI)

28.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hyd_65N_2 PF14498 97 - 161 7.9e-24 Glycosyl hydrolase family 65, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 488
AciI CCGC 3 cut(s) 208, 212, 520
AclWI GGATC 1 cut(s) 488
AcoI YGGCCR 1 cut(s) 209
AcuI CTGAAG 2 cut(s) 354, 365
AfaI GTAC 1 cut(s) 573
AgsI TTSAA 2 cut(s) 277, 566
AhdI GACNNNNNGTC 1 cut(s) 484
AluBI AGCT 3 cut(s) 168, 347, 385
AluI AGCT 3 cut(s) 168, 347, 385
AlwI GGATC 1 cut(s) 488
Aor13HI TCCGGA 1 cut(s) 488
AoxI GGCC 2 cut(s) 209, 293
Asp700I GAANNNNTTC 1 cut(s) 378
AspS9I GGNCC 3 cut(s) 18, 226, 293
AsuC2I CCSGG 1 cut(s) 417
AsuHPI GGTGA 4 cut(s) 27, 46, 73, 203
AvaII GGWCC 2 cut(s) 18, 226
BbsI GAAGAC 1 cut(s) 399
BccI CCATC 2 cut(s) 57, 182
BcnI CCSGG 1 cut(s) 417
BfaI CTAG 1 cut(s) 586
BfmI CTRYAG 1 cut(s) 216
BisI GCNGC 2 cut(s) 209, 212
BlsI GCNGC 2 cut(s) 210, 213
Bme1390I CCNGG 1 cut(s) 417
Bme18I GGWCC 2 cut(s) 18, 226
BmeRI GACNNNNNGTC 1 cut(s) 484
BmgT120I GGNCC 3 cut(s) 18, 226, 293
BmiI GGNNCC 2 cut(s) 19, 20
BmrFI CCNGG 1 cut(s) 417
BmrI ACTGGG 1 cut(s) 205
BmsI GCATC 1 cut(s) 304
BmuI ACTGGG 1 cut(s) 205
BpiI GAAGAC 1 cut(s) 399
BpuMI CCSGG 1 cut(s) 417
BsaWI WCCGGW 1 cut(s) 488
BsaXI ACNNNNNCTCC 6 cut(s) 46, 76, 336, 366, 397, 427
Bse1I ACTGG 3 cut(s) 10, 200, 412
BseAI TCCGGA 1 cut(s) 488
BseGI GGATG 2 cut(s) 262, 516
BseMII CTCAG 1 cut(s) 568
BseNI ACTGG 3 cut(s) 10, 200, 412
BseRI GAGGAG 1 cut(s) 54
BseX3I CGGCCG 1 cut(s) 209
BsgI GTGCAG 1 cut(s) 138
Bsh1285I CGRYCG 1 cut(s) 212
BshFI GGCC 2 cut(s) 211, 295
BsiEI CGRYCG 1 cut(s) 212
BsiSI CCGG 2 cut(s) 416, 489
BslFI GGGAC 1 cut(s) 31
BsmFI GGGAC 1 cut(s) 31
BsnI GGCC 2 cut(s) 211, 295
Bsp13I TCCGGA 1 cut(s) 488
Bsp143I GATC 1 cut(s) 493
BspACI CCGC 3 cut(s) 208, 212, 520
BspANI GGCC 2 cut(s) 211, 295
BspCNI CTCAG 1 cut(s) 567
BspEI TCCGGA 1 cut(s) 488
BspLI GGNNCC 2 cut(s) 19, 20
BspMAI CTGCAG 1 cut(s) 220
BspPI GGATC 1 cut(s) 488
BsrI ACTGG 3 cut(s) 10, 200, 412
BssMI GATC 1 cut(s) 493
Bst6I CTCTTC 1 cut(s) 384
BstC8I GCNNGC 1 cut(s) 216
BstDEI CTNAG 2 cut(s) 267, 554
BstF5I GGATG 2 cut(s) 262, 516
BstKTI GATC 1 cut(s) 496
BstMBI GATC 1 cut(s) 493
BstMCI CGRYCG 1 cut(s) 212
BstMWI GCNNNNNNNGC 1 cut(s) 446
BstSCI CCNGG 1 cut(s) 415
BstSFI CTRYAG 1 cut(s) 216
BstV2I GAAGAC 1 cut(s) 399
BstX2I RGATCY 1 cut(s) 493
BstYI RGATCY 1 cut(s) 493
BstZI CGGCCG 1 cut(s) 209
BsuRI GGCC 2 cut(s) 211, 295
BtsCI GGATG 2 cut(s) 262, 516
BtsIMutI CAGTG 1 cut(s) 179
Cac8I GCNNGC 1 cut(s) 216
CciNI GCGGCCGC 1 cut(s) 209
Cfr13I GGNCC 3 cut(s) 18, 226, 293
Csp6I GTAC 1 cut(s) 572
CviAII CATG 1 cut(s) 14
CviJI RGCY 8 cut(s) 168, 211, 271, 295, 340, 347, 368, 385
CviKI_1 RGCY 8 cut(s) 168, 211, 271, 295, 340, 347, 368, 385
CviQI GTAC 1 cut(s) 572
DdeI CTNAG 2 cut(s) 267, 554
DpnI GATC 1 cut(s) 495
DpnII GATC 1 cut(s) 493
DriI GACNNNNNGTC 1 cut(s) 484
EaeI YGGCCR 1 cut(s) 209
EagI CGGCCG 1 cut(s) 209
Eam1104I CTCTTC 1 cut(s) 384
Eam1105I GACNNNNNGTC 1 cut(s) 484
EarI CTCTTC 1 cut(s) 384
EclXI CGGCCG 1 cut(s) 209
Eco47I GGWCC 2 cut(s) 18, 226
Eco52I CGGCCG 1 cut(s) 209
Eco57I CTGAAG 2 cut(s) 354, 365
EcoO109I RGGNCCY 2 cut(s) 18, 226
FaeI CATG 1 cut(s) 17
FaiI YATR 4 cut(s) 15, 350, 508, 531
FaqI GGGAC 1 cut(s) 31
FatI CATG 1 cut(s) 13
Fnu4HI GCNGC 2 cut(s) 209, 212
FokI GGATG 2 cut(s) 269, 503
Fsp4HI GCNGC 2 cut(s) 209, 212
FspBI CTAG 1 cut(s) 586
GluI GCNGC 2 cut(s) 209, 212
HaeIII GGCC 2 cut(s) 211, 295
HapII CCGG 2 cut(s) 416, 489
Hin1II CATG 1 cut(s) 17
HinfI GANTC 1 cut(s) 235
HpaII CCGG 2 cut(s) 416, 489
HphI GGTGA 4 cut(s) 27, 46, 73, 203
Hpy188I TCNGA 2 cut(s) 240, 373
Hpy188III TCNNGA 4 cut(s) 232, 307, 403, 489
HpyAV CCTTC 4 cut(s) 217, 244, 271, 547
HpyCH4V TGCA 3 cut(s) 155, 218, 317
HpyF10VI GCNNNNNNNGC 1 cut(s) 446
HpyF3I CTNAG 2 cut(s) 267, 554
Hsp92II CATG 1 cut(s) 17
KflI GGGWCCC 1 cut(s) 18
Kpn2I TCCGGA 1 cut(s) 488
Kzo9I GATC 1 cut(s) 493
LmnI GCTCC 2 cut(s) 344, 445
LweI GCATC 1 cut(s) 304
MaeI CTAG 1 cut(s) 586
MaeIII GTNAC 4 cut(s) 177, 191, 280, 509
MalI GATC 1 cut(s) 495
MboI GATC 1 cut(s) 493
MboII GAAGA 2 cut(s) 371, 404
MfeI CAATTG 1 cut(s) 324
MflI RGATCY 1 cut(s) 493
MluCI AATT 4 cut(s) 109, 318, 324, 561
MlyI GAGTC 1 cut(s) 229
MnlI CCTC 7 cut(s) 67, 75, 130, 239, 247, 330, 451
MroI TCCGGA 1 cut(s) 488
MroXI GAANNNNTTC 1 cut(s) 378
MseI TTAA 1 cut(s) 108
MspI CCGG 2 cut(s) 416, 489
MspR9I CCNGG 1 cut(s) 417
MunI CAATTG 1 cut(s) 324
MwoI GCNNNNNNNGC 1 cut(s) 446
NciI CCSGG 1 cut(s) 417
NdeII GATC 1 cut(s) 493
NlaIII CATG 1 cut(s) 17
NlaIV GGNNCC 2 cut(s) 19, 20
NmuCI GTSAC 2 cut(s) 177, 191
NotI GCGGCCGC 1 cut(s) 209
PdmI GAANNNNTTC 1 cut(s) 378
PkrI GCNGC 2 cut(s) 210, 213
PleI GAGTC 1 cut(s) 229
PpsI GAGTC 1 cut(s) 229
PpuMI RGGWCCY 2 cut(s) 18, 226
Psp5II RGGWCCY 2 cut(s) 18, 226
PspN4I GGNNCC 2 cut(s) 19, 20
PspPI GGNCC 3 cut(s) 18, 226, 293
PspPPI RGGWCCY 2 cut(s) 18, 226
PstI CTGCAG 1 cut(s) 220
PsuI RGATCY 1 cut(s) 493
RsaI GTAC 1 cut(s) 573
RsaNI GTAC 1 cut(s) 572
SaqAI TTAA 1 cut(s) 108
SatI GCNGC 2 cut(s) 209, 212
Sau3AI GATC 1 cut(s) 493
Sau96I GGNCC 3 cut(s) 18, 226, 293
SchI GAGTC 1 cut(s) 229
ScrFI CCNGG 1 cut(s) 417
SetI ASST 7 cut(s) 59, 170, 231, 282, 349, 387, 462
SfaNI GCATC 1 cut(s) 304
SfcI CTRYAG 1 cut(s) 216
SinI GGWCC 2 cut(s) 18, 226
Sse9I AATT 4 cut(s) 109, 318, 324, 561
SsiI CCGC 3 cut(s) 208, 212, 520
SspI AATATT 1 cut(s) 580
SspMI CTAG 1 cut(s) 586
StyD4I CCNGG 1 cut(s) 415
TaqI TCGA 1 cut(s) 525
TasI AATT 4 cut(s) 109, 318, 324, 561
TatI WGTACW 1 cut(s) 571
TauI GCSGC 2 cut(s) 211, 214
Tru1I TTAA 1 cut(s) 108
Tru9I TTAA 1 cut(s) 108
TscAI CASTG 1 cut(s) 186
TseFI GTSAC 2 cut(s) 177, 191
Tsp45I GTSAC 2 cut(s) 177, 191
TspDTI ATGAA 2 cut(s) 405, 505
TspRI CASTG 1 cut(s) 186
VpaK11BI GGWCC 2 cut(s) 18, 226
XmnI GAANNNNTTC 1 cut(s) 378
XspI CTAG 1 cut(s) 586
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.