Rroxscaffold_7G00210690

alpha-L-fucosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0004443
Physical Location & Seq
Forward (+)
61172721 .. 61179065
6345 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00210690.1

Sequence Viewer

Length: 2472 bp
ATGGAGGATGGTGACTGGGTTCTGGTGCGGCCGCCTGCAGAGAAGGACCTCTGGACCCCAAGTTTAGTGAAGGAGGATGAGAGTTCTAAGCCTTTGAAGGTTACTTTTGGTGGGCCTGCGAAGTTCTGGACTGATGCAATTCCAATTGGGAATGGGAGGCTTGGAGCTATGGTCTGGGGTGGAGTGGCCTCAGAAACTCTTCAGCTCAATGAGGACACACTCTGGACTGGAACTCCGGGCAACTACACCAACCCAAATGCTCCACAGGCGCTAACAGAGGTCAGGAAACTTGTTGATGATGGTAAATATGTTGAAGCTACTGAAGCTGCGGTCAAGTTGTCCGGAGATCCTTCTGATGTATACCAACTACTCGGCGACATCAACCTAGAATTTGATGATTCGCATCTCAAGTATGCTGAAGAAACTTACAGCAGAGAGCTAGACCTAGATACTGCAACAGCAAGGATAAAATATTCTGTGGGTGATGTAGAATATACTAGGGAGCATTTTGCTTCGAATCCCGATCAAGTGATTGTGACAAAGATCTCTGCAAGTAAATCAGGGTCCCTATCATTCACAGTTTCTCTAGACAGCAAGATGCATCATGGTTCACATTTAAATGGTAAAAACCAAATTATACTTGAAGGAAGTTGTCCTGGTAGAAGAATTGCACCAAAGTATAATGACAATCCAGAGGGGATTCAGTTTTCTGCAGTTCTTGATTTGCAGATTAGTGGTGACAAAGGTGTGATACATGCTTTGGATGACAAGAAATTTAGAGTTGAAGAGGCAGATTGGGCTGTTTTGCTTCTGGTGGCCTCATCTTCATTTAAGGATCCATTTACTAAGCCTTCTGATTCTAAGAGAAATCCAACTTCAGAGTCTCTCACTGCATTGAATTCAATAAGAAATTTGTCATATTCTGACCTCTATGCTCATCATTTGGATGACTATCAGAAACTTTTTCATCGTGTCTCATTGCAGCTCTCTAAGAGCTCTAAGAGCATATTAGGAAATGAAACTTTAGAGATGAAGAAGCTCAACCCTATTGCCAATTTGAATTTCAAGGGAAGTGACAATGCCTTGGGTTCTACTGCTGACAGGGTGAAATCCTTTAAAACTGATGAAGATCCTTCTTTTGTGGAACTCTTGTTCCAATATGGTCGATATCTCCTTATTTCATGCTCACGGCCTGGAACTCAGCCGGCAAACCTGCAGGGTATATGGAACTACAAAATTGAACCTGCATGGGATGGTGCTCCTCATACGAACATTAATCTTCAGATGAACTATTGGCCTTCCCTTCCTTGTAATCTACGAGAATGCCAGGAACCCTTATTTGATTTCACATCTTTATTATCTATTAACGGAAGTAAAACAGCAAAGGTCAACTATGAAGCTAGTGGTTGGGTTGTGCACCAAGTGACTGACATATGGGCGAAAACATCACCAGATCGAGGTCAAGCTGTATGGGCTTTATGGCCAATGGGAGGGGCGTGGGTCTGTACTCATCTATGGGAACATTATACTTATACAATGGACAAGGATTTCCTGAAAAATAAGGCATATCCTTTGTTGGAAGGATGTGCATCATTTCTGCTGGACTGGTTGATTGAAGGACGTGGCGGGTATCTTGAAACCAACCCATCAACTTCTCCAGAGCACATGTTTATTGCTCCTGATGGTAAGCAGGCCAGCGTGAGCTACTCATCAACCATGGACATCTCAATCATAAAAGAAGTCTTTTCTGCAGTACTTTCTGCTGCTGAGGTTCTAGGAAAAACTCAAGATACTTTTGTTGAAAAAGTTCGTAATGCTCAGTCTAGGCTTCCACCCACAAAAATTGCTAGAGATGGTTCTATTCTTGAATGGGCACAAGATTTTGAGGACCCAGAAGTGCACCATCGACATGTATCACACCTGTTTGGCCTGTTTCCGGGCCACACAATAACTCTTGAAAAAACTCCAGACCTCTGTAAAGCAGTGGATTATACCCTTTTTAAAAGAGGAGAGGAAGGTCCAGGATGGTCAACTACCTGGAAGACTGCATTGTGGGCACGTCTTCACAACAGTGAACATGCGTATCGTATGGTCAAGCATTTGATTGACTTGGTAGATCCAGAACATGAAAGTGATTTTGAAGGAGGACTATACAGTAACTTGTTCACTGCACATCCTCCTTTCCAGATTGATGCCAACTTTGGCACAGTGAAGGACCTGTATTTACTTCCTGCTCTTCCTCGGGATAAATGGGCAAATGGTTGTGTCAAGGGCTTGAAGGCACGCGGCGGGGTGACAGTCAACATCTGCTGGAGTGAAGGAGATCTTCACGAAGTTGGCATTTGGTCCAAGGACAACAACACTGTTAAAAAACTACATTATAGAGGAAGTACAGTTACGGCAAACATATCGTCGGGCAGGATTTATACATTCAACAGACAGTTAAAATGCGTGAGGACAGTAGGTCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004553 GO:0004560 GO:0005575 GO:0005576 GO:0015928 GO:0016787 GO:0016798 GO:0047513 GO:0048046
KEGG Pathways
Metabolic & Signaling
ko00511 map00511
Pfam Domains
Protein Families
Glyco_hyd_65N_2

Protein Analysis

823

Amino Acids

91.87

Weight (kDa)

5.78

Isoelectric Point (pI)

27.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hyd_65N_2 PF14498 36 - 278 8.8e-64 Glycosyl hydrolase family 65, N-terminal domain
Glyco_hydro_95_cat PF22124 306 - 737 6.5e-164 Glycosyl hydrolase family 95 catalytic domain
Glyco_hydro_95_C PF21307 739 - 795 2.3e-13 Glycoside hydrolase family 95, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 1221, 1252
AccI GTMKAC 1 cut(s) 360
AccII CGCG 1 cut(s) 2286
AccIII TCCGGA 1 cut(s) 341
AciI CCGC 6 cut(s) 28, 32, 329, 1626, 2286, 2289
AclWI GGATC 5 cut(s) 341, 830, 843, 1124, 2111
AcoI YGGCCR 2 cut(s) 29, 1481
AcsI RAATTY 5 cut(s) 389, 773, 898, 910, 1060
AcuI CTGAAG 5 cut(s) 185, 342, 438, 861, 1265
AdeI CACNNNGTG 1 cut(s) 1423
AfaI GTAC 3 cut(s) 1507, 1755, 2392
AfiI CCNNNNNNNGG 3 cut(s) 1457, 1490, 1936
AflIII ACRYGT 2 cut(s) 1665, 1909
AhdI GACNNNNNGTC 1 cut(s) 2463
AjiI CACGTC 2 cut(s) 1622, 2060
AjnI CCWGG 5 cut(s) 655, 1192, 1326, 2020, 2036
AjuI GAANNNNNNNTTGG 2 cut(s) 1046, 1078
AleI CACNNNNGTG 1 cut(s) 2070
AloI GAACNNNNNNTCC 4 cut(s) 1136, 1137, 1168, 1169
Alw21I GWGCWC 5 cut(s) 998, 1261, 1419, 1665, 1902
Alw26I GTCTC 2 cut(s) 888, 979
Alw44I GTGCAC 2 cut(s) 1415, 1898
AlwI GGATC 5 cut(s) 341, 830, 843, 1124, 2111
Ama87I CYCGRG 1 cut(s) 2241
Aor13HI TCCGGA 1 cut(s) 341
ApaLI GTGCAC 2 cut(s) 1415, 1898
ApeKI GCWGC 3 cut(s) 326, 982, 1763
ApoI RAATTY 5 cut(s) 389, 773, 898, 910, 1060
ArsI GACNNNNNNTTYG 2 cut(s) 2396, 2428
AseI ATTAAT 1 cut(s) 1275
Asp700I GAANNNNTTC 2 cut(s) 198, 1806
AspLEI GCGC 1 cut(s) 271
AspS9I GGNCC 9 cut(s) 46, 54, 113, 564, 1888, 1939, 2018, 2215, 2346
AsuC2I CCSGG 2 cut(s) 237, 1938
AsuHPI GGTGA 6 cut(s) 23, 494, 749, 1117, 1440, 2305
AsuII TTCGAA 1 cut(s) 515
AvaI CYCGRG 1 cut(s) 2241
AvaII GGWCC 7 cut(s) 46, 54, 564, 1888, 2018, 2215, 2346
BaeGI GKGCMC 4 cut(s) 1419, 1876, 1902, 2059
BaeI ACNNNNGTAYC 4 cut(s) 1782, 1815, 2066, 2099
BalI TGGCCA 1 cut(s) 1483
BamHI GGATCC 1 cut(s) 835
BanII GRGCYC 1 cut(s) 998
BarI GAAGNNNNNNTAC 2 cut(s) 2380, 2412
BbsI GAAGAC 2 cut(s) 2048, 2054
Bbv12I GWGCWC 5 cut(s) 998, 1261, 1419, 1665, 1902
BbvCI CCTCAGC 1 cut(s) 1767
BbvI GCAGC 3 cut(s) 313, 994, 1750
BccI CCATC 8 cut(s) 2, 293, 1247, 1654, 1676, 1847, 1911, 2019
BceAI ACGGC 2 cut(s) 1205, 2415
BcgI CGANNNNNNTGC 2 cut(s) 2391, 2425
BciT130I CCWGG 5 cut(s) 657, 1194, 1328, 2022, 2038
BcnI CCSGG 2 cut(s) 237, 1938
BcoDI GTCTC 2 cut(s) 888, 979
BfaI CTAG 9 cut(s) 386, 440, 446, 498, 587, 1401, 1775, 1824, 1848
BfmI CTRYAG 4 cut(s) 36, 711, 1214, 1749
BfoI RGCGCY 1 cut(s) 272
BfuAI ACCTGC 2 cut(s) 1221, 1252
BglII AGATCT 2 cut(s) 543, 2323
BisI GCNGC 6 cut(s) 29, 32, 327, 983, 1764, 2287
BlsI GCNGC 6 cut(s) 30, 33, 328, 984, 1765, 2288
BmcAI AGTACT 1 cut(s) 1755
Bme1390I CCNGG 7 cut(s) 237, 657, 1194, 1328, 1938, 2022, 2038
Bme18I GGWCC 7 cut(s) 46, 54, 564, 1888, 2018, 2215, 2346
BmeRI GACNNNNNGTC 1 cut(s) 2463
BmeT110I CYCGRG 1 cut(s) 2241
BmgBI CACGTC 2 cut(s) 1622, 2060
BmgT120I GGNCC 9 cut(s) 46, 54, 113, 564, 1888, 1939, 2018, 2215, 2346
BmiI GGNNCC 6 cut(s) 56, 565, 566, 837, 1332, 1890
BmrFI CCNGG 7 cut(s) 237, 657, 1194, 1328, 1938, 2022, 2038
BmrI ACTGGG 1 cut(s) 25
BmsI GCATC 6 cut(s) 124, 412, 588, 610, 1598, 2182
BmuI ACTGGG 1 cut(s) 25
BpiI GAAGAC 2 cut(s) 2048, 2054
BpmI CTGGAG 3 cut(s) 1641, 1950, 2332
Bpu10I CCTNAGC 1 cut(s) 1767
Bpu14I TTCGAA 1 cut(s) 515
BpuEI CTTGAG 2 cut(s) 392, 1770
BpuMI CCSGG 2 cut(s) 237, 1938
BsaBI GATNNNNATC 4 cut(s) 402, 951, 1128, 1588
BsaJI CCNNGG 4 cut(s) 1083, 1716, 2240, 2349
BsaWI WCCGGW 1 cut(s) 341
BsaXI ACNNNNNCTCC 4 cut(s) 156, 186, 217, 247
Bsc4I CCNNNNNNNGG 3 cut(s) 1457, 1490, 1936
Bse118I RCCGGY 1 cut(s) 1204
Bse1I ACTGG 3 cut(s) 20, 232, 1610
Bse3DI GCAATG 1 cut(s) 977
Bse8I GATNNNNATC 4 cut(s) 402, 951, 1128, 1588
BseAI TCCGGA 1 cut(s) 341
BseBI CCWGG 5 cut(s) 657, 1194, 1328, 2022, 2038
BseDI CCNNGG 4 cut(s) 1083, 1716, 2240, 2349
BseGI GGATG 8 cut(s) 13, 82, 769, 952, 1258, 1589, 2030, 2173
BseJI GATNNNNATC 4 cut(s) 402, 951, 1128, 1588
BseLI CCNNNNNNNGG 3 cut(s) 1457, 1490, 1936
BseMI GCAATG 1 cut(s) 977
BseMII CTCAG 4 cut(s) 204, 1214, 1758, 1832
BseNI ACTGG 3 cut(s) 20, 232, 1610
BseRI GAGGAG 2 cut(s) 1251, 2022
BseSI GKGCMC 4 cut(s) 1419, 1876, 1902, 2059
BseX3I CGGCCG 1 cut(s) 29
BseXI GCAGC 3 cut(s) 313, 994, 1750
BsgI GTGCAG 1 cut(s) 2154
Bsh1236I CGCG 1 cut(s) 2286
Bsh1285I CGRYCG 1 cut(s) 32
BsiEI CGRYCG 1 cut(s) 32
BsiHKAI GWGCWC 5 cut(s) 998, 1261, 1419, 1665, 1902
BsiHKCI CYCGRG 1 cut(s) 2241
BsiSI CCGG 4 cut(s) 236, 342, 1205, 1937
BslFI GGGAC 1 cut(s) 550
BslI CCNNNNNNNGG 3 cut(s) 1457, 1490, 1936
BsmAI GTCTC 2 cut(s) 888, 979
BsmFI GGGAC 1 cut(s) 550
BsmI GAATGC 1 cut(s) 1328
BsoBI CYCGRG 1 cut(s) 2241
Bsp119I TTCGAA 1 cut(s) 515
Bsp1286I GDGCHC 7 cut(s) 998, 1261, 1419, 1665, 1876, 1902, 2059
Bsp13I TCCGGA 1 cut(s) 341
Bsp143I GATC 8 cut(s) 346, 523, 543, 835, 1129, 1453, 2116, 2323
Bsp19I CCATGG 1 cut(s) 1716
BspACI CCGC 6 cut(s) 28, 32, 329, 1626, 2286, 2289
BspCNI CTCAG 4 cut(s) 203, 1213, 1759, 1831
BspEI TCCGGA 1 cut(s) 341
BspFNI CGCG 1 cut(s) 2286
BspLI GGNNCC 6 cut(s) 56, 565, 566, 837, 1332, 1890
BspMAI CTGCAG 4 cut(s) 40, 715, 1218, 1753
BspMI ACCTGC 2 cut(s) 1221, 1252
BspPI GGATC 5 cut(s) 341, 830, 843, 1124, 2111
BspQI GCTCTTC 1 cut(s) 2241
BspT104I TTCGAA 1 cut(s) 515
BsrDI GCAATG 1 cut(s) 977
BsrFI RCCGGY 1 cut(s) 1204
BsrI ACTGG 3 cut(s) 20, 232, 1610
BssAI RCCGGY 1 cut(s) 1204
BssECI CCNNGG 4 cut(s) 1083, 1716, 2240, 2349
BssMI GATC 8 cut(s) 346, 523, 543, 835, 1129, 1453, 2116, 2323
BssNAI GTATAC 1 cut(s) 361
BssT1I CCWWGG 3 cut(s) 1083, 1716, 2349
Bst1107I GTATAC 1 cut(s) 361
Bst2UI CCWGG 5 cut(s) 657, 1194, 1328, 2022, 2038
Bst4CI ACNGT 9 cut(s) 580, 2072, 2156, 2209, 2299, 2365, 2395, 2442, 2461
Bst6I CTCTTC 3 cut(s) 204, 780, 2241
BstBI TTCGAA 1 cut(s) 515
BstC8I GCNNGC 6 cut(s) 36, 117, 1206, 1692, 1696, 2284
BstDEI CTNAG 9 cut(s) 87, 190, 846, 861, 990, 999, 1200, 1767, 1818
BstDSI CCRYGG 1 cut(s) 1716
BstF5I GGATG 8 cut(s) 13, 82, 769, 952, 1258, 1589, 2030, 2173
BstFNI CGCG 1 cut(s) 2286
BstH2I RGCGCY 1 cut(s) 272
BstHHI GCGC 1 cut(s) 271
BstKTI GATC 8 cut(s) 349, 526, 546, 838, 1132, 1456, 2119, 2326
BstMAI GTCTC 2 cut(s) 888, 979
BstMBI GATC 8 cut(s) 346, 523, 543, 835, 1129, 1453, 2116, 2323
BstMCI CGRYCG 1 cut(s) 32
BstMWI GCNNNNNNNGC 6 cut(s) 266, 323, 797, 1002, 1472, 2054
BstNI CCWGG 5 cut(s) 657, 1194, 1328, 2022, 2038
BstNSI RCATGY 4 cut(s) 758, 1669, 1913, 2081
BstSCI CCNGG 7 cut(s) 235, 655, 1192, 1326, 1936, 2020, 2036
BstSFI CTRYAG 4 cut(s) 36, 711, 1214, 1749
BstSLI GKGCMC 4 cut(s) 1419, 1876, 1902, 2059
BstUI CGCG 1 cut(s) 2286
BstV1I GCAGC 3 cut(s) 313, 994, 1750
BstV2I GAAGAC 2 cut(s) 2048, 2054
BstX2I RGATCY 6 cut(s) 346, 543, 835, 1129, 2116, 2323
BstYI RGATCY 6 cut(s) 346, 543, 835, 1129, 2116, 2323
BstZ17I GTATAC 1 cut(s) 361
BstZI CGGCCG 1 cut(s) 29
BtgI CCRYGG 1 cut(s) 1716
BtrI CACGTC 2 cut(s) 1622, 2060
BtsCI GGATG 8 cut(s) 13, 82, 769, 952, 1258, 1589, 2030, 2173
BtsI GCAGTG 3 cut(s) 888, 1989, 2166
BtsIMutI CAGTG 6 cut(s) 888, 1989, 2077, 2166, 2214, 2361
BveI ACCTGC 2 cut(s) 1221, 1252
Cac8I GCNNGC 6 cut(s) 36, 117, 1206, 1692, 1696, 2284
CciNI GCGGCCGC 1 cut(s) 29
CfoI GCGC 1 cut(s) 271
Cfr10I RCCGGY 1 cut(s) 1204
Cfr13I GGNCC 9 cut(s) 46, 54, 113, 564, 1888, 1939, 2018, 2215, 2346
Csp6I GTAC 3 cut(s) 1506, 1754, 2391
CspCI CAANNNNNGTGG 2 cut(s) 1825, 1860
CviAII CATG 9 cut(s) 605, 755, 1182, 1248, 1666, 1717, 1910, 2078, 2126
CviQI GTAC 3 cut(s) 1506, 1754, 2391
DdeI CTNAG 9 cut(s) 87, 190, 846, 861, 990, 999, 1200, 1767, 1818
DpnI GATC 8 cut(s) 348, 525, 545, 837, 1131, 1455, 2118, 2325
DpnII GATC 8 cut(s) 346, 523, 543, 835, 1129, 1453, 2116, 2323
DraI TTTAAA 3 cut(s) 618, 1117, 2002
DraIII CACNNNGTG 1 cut(s) 1423
DriI GACNNNNNGTC 1 cut(s) 2463
EaeI YGGCCR 2 cut(s) 29, 1481
EagI CGGCCG 1 cut(s) 29
Eam1104I CTCTTC 3 cut(s) 204, 780, 2241
Eam1105I GACNNNNNGTC 1 cut(s) 2463
EarI CTCTTC 3 cut(s) 204, 780, 2241
Ecl136II GAGCTC 1 cut(s) 996
EclXI CGGCCG 1 cut(s) 29
Eco130I CCWWGG 3 cut(s) 1083, 1716, 2349
Eco24I GRGCYC 1 cut(s) 998
Eco32I GATATC 1 cut(s) 1169
Eco47I GGWCC 7 cut(s) 46, 54, 564, 1888, 2018, 2215, 2346
Eco52I CGGCCG 1 cut(s) 29
Eco53kI GAGCTC 1 cut(s) 996
Eco57I CTGAAG 5 cut(s) 185, 342, 438, 861, 1265
Eco88I CYCGRG 1 cut(s) 2241
EcoICRI GAGCTC 1 cut(s) 996
EcoO109I RGGNCCY 4 cut(s) 46, 564, 1888, 2215
EcoRI GAATTC 1 cut(s) 898
EcoRII CCWGG 5 cut(s) 655, 1192, 1326, 2020, 2036
EcoRV GATATC 1 cut(s) 1169
EcoT14I CCWWGG 3 cut(s) 1083, 1716, 2349
EcoT22I ATGCAT 1 cut(s) 603
EcoT38I GRGCYC 1 cut(s) 998
ErhI CCWWGG 3 cut(s) 1083, 1716, 2349
FaeI CATG 9 cut(s) 608, 758, 1185, 1251, 1669, 1720, 1913, 2081, 2129
FalI AAGNNNNNCTT 2 cut(s) 2310, 2342
FaqI GGGAC 1 cut(s) 550
FatI CATG 9 cut(s) 604, 754, 1181, 1247, 1665, 1716, 1909, 2077, 2125
FauI CCCGC 2 cut(s) 1619, 2282
FauNDI CATATG 1 cut(s) 1433
FblI GTMKAC 1 cut(s) 360
Fnu4HI GCNGC 6 cut(s) 29, 32, 327, 983, 1764, 2287
FokI GGATG 8 cut(s) 20, 89, 776, 959, 1265, 1596, 2037, 2160
FriOI GRGCYC 1 cut(s) 998
Fsp4HI GCNGC 6 cut(s) 29, 32, 327, 983, 1764, 2287
FspBI CTAG 9 cut(s) 386, 440, 446, 498, 587, 1401, 1775, 1824, 1848
GlaI GCGC 1 cut(s) 270
GluI GCNGC 6 cut(s) 29, 32, 327, 983, 1764, 2287
GsuI CTGGAG 3 cut(s) 1641, 1950, 2332
HaeII RGCGCY 1 cut(s) 272
HapII CCGG 4 cut(s) 236, 342, 1205, 1937
HhaI GCGC 1 cut(s) 271
Hin1II CATG 9 cut(s) 608, 758, 1185, 1251, 1669, 1720, 1913, 2081, 2129
Hin6I GCGC 1 cut(s) 269
HinP1I GCGC 1 cut(s) 269
HincII GTYRAC 3 cut(s) 1390, 2031, 2302
HindII GTYRAC 3 cut(s) 1390, 2031, 2302
HinfI GANTC 5 cut(s) 398, 517, 700, 857, 881
HpaII CCGG 4 cut(s) 236, 342, 1205, 1937
HphI GGTGA 6 cut(s) 23, 494, 749, 1117, 1440, 2305
Hpy166II GTNNAC 9 cut(s) 361, 611, 1390, 1417, 1900, 2031, 2075, 2166, 2302
Hpy188I TCNGA 7 cut(s) 193, 355, 856, 880, 925, 957, 1284
Hpy8I GTNNAC 9 cut(s) 361, 611, 1390, 1417, 1900, 2031, 2075, 2166, 2302
Hpy99I CGWCG 1 cut(s) 2416
HpyCH4III ACNGT 9 cut(s) 580, 2072, 2156, 2209, 2299, 2365, 2395, 2442, 2461
HpyCH4IV ACGT 2 cut(s) 1621, 2059
HpyF10VI GCNNNNNNNGC 6 cut(s) 266, 323, 797, 1002, 1472, 2054
HpyF3I CTNAG 9 cut(s) 87, 190, 846, 861, 990, 999, 1200, 1767, 1818
HpySE526I ACGT 2 cut(s) 1621, 2059
Hsp92II CATG 9 cut(s) 608, 758, 1185, 1251, 1669, 1720, 1913, 2081, 2129
HspAI GCGC 1 cut(s) 269
KflI GGGWCCC 1 cut(s) 564
Kpn2I TCCGGA 1 cut(s) 341
KroI GCCGGC 1 cut(s) 1204
KroNI GCCGGC 1 cut(s) 1206
Kzo9I GATC 8 cut(s) 346, 523, 543, 835, 1129, 1453, 2116, 2323
LguI GCTCTTC 1 cut(s) 2241
LmnI GCTCC 5 cut(s) 164, 265, 502, 1264, 1681
Lsp1109I GCAGC 3 cut(s) 313, 994, 1750
LweI GCATC 6 cut(s) 124, 412, 588, 610, 1598, 2182
MaeI CTAG 9 cut(s) 386, 440, 446, 498, 587, 1401, 1775, 1824, 1848
MaeII ACGT 2 cut(s) 1621, 2059
MaeIII GTNAC 9 cut(s) 11, 100, 535, 737, 1073, 1423, 2156, 2293, 2395
MalI GATC 8 cut(s) 348, 525, 545, 837, 1131, 1455, 2118, 2325
MboI GATC 8 cut(s) 346, 523, 543, 835, 1129, 1453, 2116, 2323
MfeI CAATTG 1 cut(s) 144
MflI RGATCY 6 cut(s) 346, 543, 835, 1129, 2116, 2323
MhlI GDGCHC 7 cut(s) 998, 1261, 1419, 1665, 1876, 1902, 2059
MlsI TGGCCA 1 cut(s) 1483
MluNI TGGCCA 1 cut(s) 1483
MlyI GAGTC 1 cut(s) 890
MmeI TCCRAC 2 cut(s) 896, 1557
Mox20I TGGCCA 1 cut(s) 1483
Mph1103I ATGCAT 1 cut(s) 603
MroI TCCGGA 1 cut(s) 341
MroNI GCCGGC 1 cut(s) 1204
MroXI GAANNNNTTC 2 cut(s) 198, 1806
MscI TGGCCA 1 cut(s) 1483
MseI TTAA 8 cut(s) 617, 831, 1116, 1275, 1365, 2001, 2367, 2444
MslI CAYNNNNRTG 5 cut(s) 618, 945, 1908, 2070, 2130
Msp20I TGGCCA 1 cut(s) 1483
MspI CCGG 4 cut(s) 236, 342, 1205, 1937
MspR9I CCNGG 7 cut(s) 237, 657, 1194, 1328, 1938, 2022, 2038
MunI CAATTG 1 cut(s) 144
Mva1269I GAATGC 1 cut(s) 1328
MvaI CCWGG 5 cut(s) 657, 1194, 1328, 2022, 2038
MvnI CGCG 1 cut(s) 2286
MwoI GCNNNNNNNGC 6 cut(s) 266, 323, 797, 1002, 1472, 2054
NaeI GCCGGC 1 cut(s) 1206
NciI CCSGG 2 cut(s) 237, 1938
NcoI CCATGG 1 cut(s) 1716
NdeI CATATG 1 cut(s) 1433
NdeII GATC 8 cut(s) 346, 523, 543, 835, 1129, 1453, 2116, 2323
NgoMIV GCCGGC 1 cut(s) 1204
NlaIII CATG 9 cut(s) 608, 758, 1185, 1251, 1669, 1720, 1913, 2081, 2129
NlaIV GGNNCC 6 cut(s) 56, 565, 566, 837, 1332, 1890
NmeAIII GCCGAG 1 cut(s) 351
NmuCI GTSAC 6 cut(s) 11, 535, 737, 1073, 1423, 2293
NotI GCGGCCGC 1 cut(s) 29
NsiI ATGCAT 1 cut(s) 603
NspI RCATGY 4 cut(s) 758, 1669, 1913, 2081
NspV TTCGAA 1 cut(s) 515
OliI CACNNNNGTG 1 cut(s) 2070
PciI ACATGT 2 cut(s) 1665, 1909
PciSI GCTCTTC 1 cut(s) 2241
PctI GAATGC 1 cut(s) 1328
PdiI GCCGGC 1 cut(s) 1206
PdmI GAANNNNTTC 2 cut(s) 198, 1806
PfeI GAWTC 4 cut(s) 398, 517, 700, 857
PfoI TCCNGGA 1 cut(s) 2020
PkrI GCNGC 6 cut(s) 30, 33, 328, 984, 1765, 2288
PleI GAGTC 1 cut(s) 889
PpsI GAGTC 1 cut(s) 889
PpuMI RGGWCCY 4 cut(s) 46, 564, 1888, 2215
PscI ACATGT 2 cut(s) 1665, 1909
PshBI ATTAAT 1 cut(s) 1275
Psp124BI GAGCTC 1 cut(s) 998
Psp5II RGGWCCY 4 cut(s) 46, 564, 1888, 2215
Psp6I CCWGG 5 cut(s) 655, 1192, 1326, 2020, 2036
PspGI CCWGG 5 cut(s) 655, 1192, 1326, 2020, 2036
PspN4I GGNNCC 6 cut(s) 56, 565, 566, 837, 1332, 1890
PspPI GGNCC 9 cut(s) 46, 54, 113, 564, 1888, 1939, 2018, 2215, 2346
PspPPI RGGWCCY 4 cut(s) 46, 564, 1888, 2215
PstI CTGCAG 4 cut(s) 40, 715, 1218, 1753
PsuI RGATCY 6 cut(s) 346, 543, 835, 1129, 2116, 2323
RsaI GTAC 3 cut(s) 1507, 1755, 2392
RsaNI GTAC 3 cut(s) 1506, 1754, 2391
RseI CAYNNNNRTG 5 cut(s) 618, 945, 1908, 2070, 2130
SacI GAGCTC 1 cut(s) 998
SapI GCTCTTC 1 cut(s) 2241
SaqAI TTAA 8 cut(s) 617, 831, 1116, 1275, 1365, 2001, 2367, 2444
SatI GCNGC 6 cut(s) 29, 32, 327, 983, 1764, 2287
Sau3AI GATC 8 cut(s) 346, 523, 543, 835, 1129, 1453, 2116, 2323
Sau96I GGNCC 9 cut(s) 46, 54, 113, 564, 1888, 1939, 2018, 2215, 2346
SbfI CCTGCAGG 1 cut(s) 1218
ScaI AGTACT 1 cut(s) 1755
SchI GAGTC 1 cut(s) 890
ScrFI CCNGG 7 cut(s) 237, 657, 1194, 1328, 1938, 2022, 2038
SdaI CCTGCAGG 1 cut(s) 1218
SduI GDGCHC 7 cut(s) 998, 1261, 1419, 1665, 1876, 1902, 2059
SfaNI GCATC 6 cut(s) 124, 412, 588, 610, 1598, 2182
SfcI CTRYAG 4 cut(s) 36, 711, 1214, 1749
SfuI TTCGAA 1 cut(s) 515
SinI GGWCC 7 cut(s) 46, 54, 564, 1888, 2018, 2215, 2346
SmiI ATTTAAAT 1 cut(s) 618
SmiMI CAYNNNNRTG 5 cut(s) 618, 945, 1908, 2070, 2130
SmlI CTYRAG 2 cut(s) 407, 1785
SmoI CTYRAG 2 cut(s) 407, 1785
Sse8387I CCTGCAGG 1 cut(s) 1218
SsiI CCGC 6 cut(s) 28, 32, 329, 1626, 2286, 2289
SspI AATATT 1 cut(s) 473
SspMI CTAG 9 cut(s) 386, 440, 446, 498, 587, 1401, 1775, 1824, 1848
SstI GAGCTC 1 cut(s) 998
StyD4I CCNGG 7 cut(s) 235, 655, 1192, 1326, 1936, 2020, 2036
StyI CCWWGG 3 cut(s) 1083, 1716, 2349
SwaI ATTTAAAT 1 cut(s) 618
TaaI ACNGT 9 cut(s) 580, 2072, 2156, 2209, 2299, 2365, 2395, 2442, 2461
TaiI ACGT 2 cut(s) 1624, 2062
TaqI TCGA 4 cut(s) 515, 1165, 1456, 1906
TatI WGTACW 3 cut(s) 1505, 1753, 2390
TauI GCSGC 3 cut(s) 31, 34, 2289
TfiI GAWTC 4 cut(s) 398, 517, 700, 857
Tru1I TTAA 8 cut(s) 617, 831, 1116, 1275, 1365, 2001, 2367, 2444
Tru9I TTAA 8 cut(s) 617, 831, 1116, 1275, 1365, 2001, 2367, 2444
TscAI CASTG 6 cut(s) 895, 1989, 2077, 2173, 2214, 2368
TseFI GTSAC 6 cut(s) 11, 535, 737, 1073, 1423, 2293
TseI GCWGC 3 cut(s) 326, 982, 1763
Tsp45I GTSAC 6 cut(s) 11, 535, 737, 1073, 1423, 2293
TspDTI ATGAA 9 cut(s) 816, 956, 1032, 1046, 1140, 1170, 1301, 1410, 2142
TspGWI ACGGA 1 cut(s) 1383
TspRI CASTG 6 cut(s) 895, 1989, 2077, 2173, 2214, 2368
VneI GTGCAC 2 cut(s) 1415, 1898
VpaK11BI GGWCC 7 cut(s) 46, 54, 564, 1888, 2018, 2215, 2346
VspI ATTAAT 1 cut(s) 1275
XapI RAATTY 5 cut(s) 389, 773, 898, 910, 1060
XbaI TCTAGA 1 cut(s) 586
XceI RCATGY 4 cut(s) 758, 1669, 1913, 2081
XmiI GTMKAC 1 cut(s) 360
XmnI GAANNNNTTC 2 cut(s) 198, 1806
XspI CTAG 9 cut(s) 386, 440, 446, 498, 587, 1401, 1775, 1824, 1848
ZrmI AGTACT 1 cut(s) 1755
Zsp2I ATGCAT 1 cut(s) 603
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.