Rh6CG062600

alpha-L-fucosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0004443
Physical Location & Seq
Reverse (-)
6187172 .. 6193694
6523 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG062600.1

Sequence Viewer

Length: 2523 bp
ATGGAGGATGGTGACTGGGTTCTGGTGCGGCCGCCTGCAGAGAAGGACCTCTGGACCCCAAGTTTAGTGAAGGAGGATGACAGTTCTAGGCCTTTGAAGGTTACTTTTGGTGGGCCTGCGAAGTTCTGGACTGATGCAATTCCAATTGGGAATGGGAGGCTTGGAGCTATGGTCTGGGGTGGTGTGGCTTCTGAAACTCTTCAGCTCAATGTTTCAATTGGTGTAGAGGACACACTCTGGACTGGAACTCCAGGCAACTACACCAACCCAAATGCTCCACAGGCGCTAACAGAGGTTAGGAAACTTGTTGATGATGGTAACTATGTTGAAGCTACTGAAGCAGCGGTCAAGTTGACTGGAGATCCTTCTGATGTATACCAACTACTCGGCGACATCAACCTAGAATTTGATGATTCGCATCTCAAGTATGCTGAAGAAACTTACAGCAGAGAGCTAGACCTAGATACTGCAACAGCAAGGATAAAATATTCTGTGGGGGATGTAGAATATACTAGGGAGCATTTTGCTTCGAACCCCGATCAAGTGATTGTGACAAAGATCTCTGCAAGCAAATCAGGGTCCCTATCATTTACAGTTTCTCTAGACAGCAAGATGCATCATGGTTCACATCTAAATGGTAAAAACCAAATTATACTTGAAGGAAGTTGTCCTGGTAAAAGAATTCCACCAAAGTATAATGAAATTCCAAAGGGGATTCAGTTTACTGCAGTTCTTGATTTGCAGATTAGTGGTGACAAAGGTGTGATACATGCTTTGGATGACAAGAAATTTAGAGTTGAAAAAGCAGATTGGGCTGTTTTGCTTCTGGTGGCCTCATCTTCATTTAAAGATCCATTTACTAAGCCCTCTGATTCTAAGAGAAATCCAACTTCAGAGTCTCTCACTGCATTGAATTCAATAAGAAATTTGTCATATTCTGACCTCTATGCTCATCATTTGGATGACTATCAGAATCTTTTTCATCGTGTCTCATTGCAGCTCTCTAAGAGCTCTAAGAGCATATTAGGAAATAAAACTTTGGAGATGAAGAAGCTCAACCCTATTACTAATTTGAATTTCAAGGGAAGTGACAATGCCTTGGGTTCTACTGCTGACAGGGTGAAATCCTTTAAAACTGATGAAGATCCTTCTTTTGTGGAACTCTTGTTCCAATATGGTCGATATCTTCTTATTTCATGCTCACGGCCTGGAACTCAGCCGGCAAACCTGCAGGGTATATGGAACTACAAAATTGAACCTGCATGGGATGGTGCTCCTCATACGAACATTAATCTTCAGATGAACTATTGGCCTTCCCTTCCTTGTAATCTACGAGAATGCCAGGAACCCTTATTTGATTACACATCTTTATTATCTATTAACGGAAGTAAAACAGCAAAGGTCAACTACGAAGCTAGTGGTTGGGTTGTTCATCAAGTGACTGACATATGGGCGAAAACATCACCAGATCGAGGTCAAGCTGTATGGGCTTTATGGCCAATGGGAGGAGCGTGGATCTGTACTCATCTATGGGAACATTATACTTATACAATGGACAAAGATTTCCTGAAAAATAAGGCATATCCTTTGTTGGAAGGATGTGCATCATTTCTACTGGACTGGTTGATTGAAGGACGTGGCGGGTATCTTGAAACCAACCCATCAACTTCTCCAGAGCACATGTTTATTGCTCCTGATGGTAAACAGGCCAGTGTGAGCTACTCATCAACCATGGACATCTCAATCATAAAAGAAGTATTTTCTGCAATACTTTCTGCTGCTGAGGTTTTAGGAAAAACTCAAGATGCTTTTATTGAAAAAGTTCGCAATGCTCAATCTAGGCTTCCACCCACAAAAATTGCTAGAGATGGTTCTATTATGGAATGGGCACAAGATTTTGAGGACCCAGAAGTGCACCATCGACATGTATCACACCTGTTTGGCCTCTTTCCCGGTCACACAATAACTCTTGAAAAAAATCCAGACCTCTGTAAAGCAGTGGATTATACCCTTTTTAAAAGAGGAGAGGAAGGTCCAGGATGGTCAACTACATGGAAGACTGCATTGTGGGCACGTCTTCACAACAGTGAACATGCGTATCGTATGGTCAAGCATTTGATTGACTTGGTAGATCCAGAACATGAAGGTGATTTTGAAGGAGGACTATACAGTAACTTGTTCACTGCACATCCTCCTTTCCAGATTGATGCCAACTTTGGTTTTTCAGCAGCAGTTGCCGAAATGCTTGTCCAAAGCACTGTCAAGGACTTGTATTTACTTCCTGCTCTTCCCCGGGATAAATGGGCAAATGGTTGTGTTAAAGGCTTGAAGGCACGCGGCGGGGTGACAGTCAACATCTGCTGGAGCGAAGGAGATCTTCACGAAGTTGGCATTTGGTCCAAGGACAACAACTCTGTTCAAAAACTACATTATAGAGGAAGTACAGTTACGGCAAACATATTGTCCGGCAAGATTTATACATTCAACAGACAGTTAAAATGTGTGAGGACAGTAAGTCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004553 GO:0004560 GO:0005575 GO:0005576 GO:0015928 GO:0016787 GO:0016798 GO:0047513 GO:0048046
KEGG Pathways
Metabolic & Signaling
ko00511 map00511
Pfam Domains
Protein Families
Glyco_hyd_65N_2

Protein Analysis

840

Amino Acids

93.66

Weight (kDa)

5.87

Isoelectric Point (pI)

28.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hyd_65N_2 PF14498 36 - 283 4.1e-60 Glycosyl hydrolase family 65, N-terminal domain
Glyco_hydro_95_cat PF22124 311 - 749 8.2e-169 Glycosyl hydrolase family 95 catalytic domain
Glyco_hydro_95_C PF21307 751 - 808 2.7e-14 Glycoside hydrolase family 95, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 1236, 1267
AccI GTMKAC 1 cut(s) 375
AccII CGCG 1 cut(s) 2337
AciI CCGC 6 cut(s) 28, 32, 344, 1641, 2337, 2340
AclWI GGATC 5 cut(s) 356, 845, 1139, 1523, 2126
AcoI YGGCCR 2 cut(s) 29, 1496
AcsI RAATTY 7 cut(s) 404, 681, 702, 788, 913, 925, 1075
AcuI CTGAAG 5 cut(s) 185, 357, 453, 876, 1280
AfaI GTAC 2 cut(s) 1522, 2443
AfiI CCNNNNNNNGG 2 cut(s) 1472, 1505
AflIII ACRYGT 2 cut(s) 1680, 1924
AhdI GACNNNNNGTC 1 cut(s) 2514
AjiI CACGTC 2 cut(s) 1637, 2075
AjnI CCWGG 5 cut(s) 250, 670, 1207, 1341, 2035
AleI CACNNNNGTG 1 cut(s) 2085
AloI GAACNNNNNNTCC 4 cut(s) 1151, 1152, 1183, 1184
Alw21I GWGCWC 4 cut(s) 1013, 1276, 1680, 1917
Alw26I GTCTC 2 cut(s) 903, 994
Alw44I GTGCAC 1 cut(s) 1913
AlwI GGATC 5 cut(s) 356, 845, 1139, 1523, 2126
Ama87I CYCGRG 1 cut(s) 2292
AoxI GGCC 9 cut(s) 29, 89, 113, 831, 1205, 1310, 1496, 1707, 1942
ApaLI GTGCAC 1 cut(s) 1913
ApeKI GCWGC 4 cut(s) 341, 997, 1778, 2228
ApoI RAATTY 7 cut(s) 404, 681, 702, 788, 913, 925, 1075
ArsI GACNNNNNNTTYG 4 cut(s) 2232, 2264, 2447, 2479
AseI ATTAAT 1 cut(s) 1290
Asp700I GAANNNNTTC 2 cut(s) 198, 1821
AspLEI GCGC 1 cut(s) 286
AspS9I GGNCC 7 cut(s) 46, 54, 113, 579, 1903, 2033, 2397
AsuC2I CCSGG 3 cut(s) 1953, 2293, 2294
AsuHPI GGTGA 6 cut(s) 23, 764, 1132, 1455, 2159, 2356
AsuII TTCGAA 1 cut(s) 530
AvaI CYCGRG 1 cut(s) 2292
AvaII GGWCC 6 cut(s) 46, 54, 579, 1903, 2033, 2397
BaeGI GKGCMC 3 cut(s) 1891, 1917, 2074
BaeI ACNNNNGTAYC 2 cut(s) 2081, 2114
BalI TGGCCA 1 cut(s) 1498
BanII GRGCYC 1 cut(s) 1013
BarI GAAGNNNNNNTAC 2 cut(s) 2431, 2463
BbsI GAAGAC 2 cut(s) 2063, 2069
Bbv12I GWGCWC 4 cut(s) 1013, 1276, 1680, 1917
BbvCI CCTCAGC 1 cut(s) 1782
BbvI GCAGC 4 cut(s) 353, 1009, 1765, 2240
BccI CCATC 8 cut(s) 2, 308, 1262, 1669, 1691, 1862, 1926, 2034
BceAI ACGGC 2 cut(s) 1220, 2466
BciT130I CCWGG 5 cut(s) 252, 672, 1209, 1343, 2037
BcnI CCSGG 3 cut(s) 1953, 2293, 2294
BcoDI GTCTC 2 cut(s) 903, 994
BfaI CTAG 9 cut(s) 87, 401, 455, 461, 513, 602, 1416, 1839, 1863
BfmI CTRYAG 3 cut(s) 36, 726, 1229
BfoI RGCGCY 1 cut(s) 287
BfuAI ACCTGC 2 cut(s) 1236, 1267
BglII AGATCT 2 cut(s) 558, 2374
BisI GCNGC 7 cut(s) 29, 32, 342, 998, 1779, 2229, 2338
BlsI GCNGC 7 cut(s) 30, 33, 343, 999, 1780, 2230, 2339
Bme1390I CCNGG 8 cut(s) 252, 672, 1209, 1343, 1953, 2037, 2293, 2294
Bme18I GGWCC 6 cut(s) 46, 54, 579, 1903, 2033, 2397
BmeRI GACNNNNNGTC 1 cut(s) 2514
BmeT110I CYCGRG 1 cut(s) 2292
BmgBI CACGTC 2 cut(s) 1637, 2075
BmgT120I GGNCC 7 cut(s) 46, 54, 113, 579, 1903, 2033, 2397
BmiI GGNNCC 5 cut(s) 56, 580, 581, 1347, 1905
BmrFI CCNGG 8 cut(s) 252, 672, 1209, 1343, 1953, 2037, 2293, 2294
BmrI ACTGGG 1 cut(s) 25
BmsI GCATC 7 cut(s) 124, 427, 603, 625, 1613, 1795, 2197
BmuI ACTGGG 1 cut(s) 25
BpiI GAAGAC 2 cut(s) 2063, 2069
BpmI CTGGAG 4 cut(s) 234, 378, 1656, 2383
Bpu10I CCTNAGC 1 cut(s) 1782
Bpu14I TTCGAA 1 cut(s) 530
BpuEI CTTGAG 2 cut(s) 407, 1785
BpuMI CCSGG 3 cut(s) 1953, 2293, 2294
BsaBI GATNNNNATC 4 cut(s) 417, 966, 1143, 1603
BsaJI CCNNGG 5 cut(s) 1098, 1731, 2291, 2292, 2400
BsaXI ACNNNNNCTCC 4 cut(s) 156, 186, 232, 262
Bsc4I CCNNNNNNNGG 2 cut(s) 1472, 1505
Bse118I RCCGGY 1 cut(s) 1219
Bse1I ACTGG 6 cut(s) 20, 247, 361, 1620, 1625, 1710
Bse3DI GCAATG 2 cut(s) 992, 1834
Bse8I GATNNNNATC 4 cut(s) 417, 966, 1143, 1603
BseBI CCWGG 5 cut(s) 252, 672, 1209, 1343, 2037
BseDI CCNNGG 5 cut(s) 1098, 1731, 2291, 2292, 2400
BseGI GGATG 9 cut(s) 13, 82, 505, 784, 967, 1273, 1604, 2045, 2188
BseJI GATNNNNATC 4 cut(s) 417, 966, 1143, 1603
BseLI CCNNNNNNNGG 2 cut(s) 1472, 1505
BseMI GCAATG 2 cut(s) 992, 1834
BseMII CTCAG 2 cut(s) 1229, 1773
BseNI ACTGG 6 cut(s) 20, 247, 361, 1620, 1625, 1710
BseRI GAGGAG 3 cut(s) 1266, 1521, 2037
BseSI GKGCMC 3 cut(s) 1891, 1917, 2074
BseX3I CGGCCG 1 cut(s) 29
BseXI GCAGC 4 cut(s) 353, 1009, 1765, 2240
BsgI GTGCAG 1 cut(s) 2169
Bsh1236I CGCG 1 cut(s) 2337
Bsh1285I CGRYCG 1 cut(s) 32
BshFI GGCC 9 cut(s) 31, 91, 115, 833, 1207, 1312, 1498, 1709, 1944
BsiEI CGRYCG 1 cut(s) 32
BsiHKAI GWGCWC 4 cut(s) 1013, 1276, 1680, 1917
BsiHKCI CYCGRG 1 cut(s) 2292
BsiSI CCGG 4 cut(s) 1220, 1953, 2293, 2466
BslFI GGGAC 1 cut(s) 565
BslI CCNNNNNNNGG 2 cut(s) 1472, 1505
BsmAI GTCTC 2 cut(s) 903, 994
BsmFI GGGAC 1 cut(s) 565
BsmI GAATGC 1 cut(s) 1343
BsnI GGCC 9 cut(s) 31, 91, 115, 833, 1207, 1312, 1498, 1709, 1944
BsoBI CYCGRG 1 cut(s) 2292
Bsp119I TTCGAA 1 cut(s) 530
Bsp1286I GDGCHC 6 cut(s) 1013, 1276, 1680, 1891, 1917, 2074
Bsp143I GATC 9 cut(s) 361, 538, 558, 850, 1144, 1468, 1515, 2131, 2374
Bsp19I CCATGG 1 cut(s) 1731
BspACI CCGC 6 cut(s) 28, 32, 344, 1641, 2337, 2340
BspANI GGCC 9 cut(s) 31, 91, 115, 833, 1207, 1312, 1498, 1709, 1944
BspCNI CTCAG 2 cut(s) 1228, 1774
BspFNI CGCG 1 cut(s) 2337
BspLI GGNNCC 5 cut(s) 56, 580, 581, 1347, 1905
BspMAI CTGCAG 3 cut(s) 40, 730, 1233
BspMI ACCTGC 2 cut(s) 1236, 1267
BspPI GGATC 5 cut(s) 356, 845, 1139, 1523, 2126
BspQI GCTCTTC 1 cut(s) 2292
BspT104I TTCGAA 1 cut(s) 530
BsrDI GCAATG 2 cut(s) 992, 1834
BsrFI RCCGGY 1 cut(s) 1219
BsrI ACTGG 6 cut(s) 20, 247, 361, 1620, 1625, 1710
BssAI RCCGGY 1 cut(s) 1219
BssECI CCNNGG 5 cut(s) 1098, 1731, 2291, 2292, 2400
BssMI GATC 9 cut(s) 361, 538, 558, 850, 1144, 1468, 1515, 2131, 2374
BssNAI GTATAC 1 cut(s) 376
BssT1I CCWWGG 3 cut(s) 1098, 1731, 2400
Bst1107I GTATAC 1 cut(s) 376
Bst2UI CCWGG 5 cut(s) 252, 672, 1209, 1343, 2037
Bst4CI ACNGT 9 cut(s) 83, 595, 2087, 2171, 2260, 2350, 2446, 2493, 2512
Bst6I CTCTTC 2 cut(s) 204, 2292
BstAPI GCANNNNNTGC 1 cut(s) 2234
BstBI TTCGAA 1 cut(s) 530
BstC8I GCNNGC 5 cut(s) 36, 117, 568, 1221, 2335
BstDEI CTNAG 6 cut(s) 861, 876, 1005, 1014, 1215, 1782
BstDSI CCRYGG 1 cut(s) 1731
BstF5I GGATG 9 cut(s) 13, 82, 505, 784, 967, 1273, 1604, 2045, 2188
BstFNI CGCG 1 cut(s) 2337
BstH2I RGCGCY 1 cut(s) 287
BstHHI GCGC 1 cut(s) 286
BstKTI GATC 9 cut(s) 364, 541, 561, 853, 1147, 1471, 1518, 2134, 2377
BstMAI GTCTC 2 cut(s) 903, 994
BstMBI GATC 9 cut(s) 361, 538, 558, 850, 1144, 1468, 1515, 2131, 2374
BstMCI CGRYCG 1 cut(s) 32
BstMWI GCNNNNNNNGC 7 cut(s) 281, 338, 812, 1017, 1487, 2069, 2234
BstNI CCWGG 5 cut(s) 252, 672, 1209, 1343, 2037
BstNSI RCATGY 4 cut(s) 773, 1684, 1928, 2096
BstSCI CCNGG 8 cut(s) 250, 670, 1207, 1341, 1951, 2035, 2291, 2292
BstSFI CTRYAG 3 cut(s) 36, 726, 1229
BstSLI GKGCMC 3 cut(s) 1891, 1917, 2074
BstUI CGCG 1 cut(s) 2337
BstV1I GCAGC 4 cut(s) 353, 1009, 1765, 2240
BstV2I GAAGAC 2 cut(s) 2063, 2069
BstX2I RGATCY 7 cut(s) 361, 558, 850, 1144, 1515, 2131, 2374
BstYI RGATCY 7 cut(s) 361, 558, 850, 1144, 1515, 2131, 2374
BstZ17I GTATAC 1 cut(s) 376
BstZI CGGCCG 1 cut(s) 29
BsuRI GGCC 9 cut(s) 31, 91, 115, 833, 1207, 1312, 1498, 1709, 1944
BtgI CCRYGG 1 cut(s) 1731
BtrI CACGTC 2 cut(s) 1637, 2075
BtsCI GGATG 9 cut(s) 13, 82, 505, 784, 967, 1273, 1604, 2045, 2188
BtsI GCAGTG 3 cut(s) 903, 2004, 2181
BtsIMutI CAGTG 6 cut(s) 903, 1717, 2004, 2092, 2181, 2256
BveI ACCTGC 2 cut(s) 1236, 1267
Cac8I GCNNGC 5 cut(s) 36, 117, 568, 1221, 2335
CciNI GCGGCCGC 1 cut(s) 29
CfoI GCGC 1 cut(s) 286
Cfr10I RCCGGY 1 cut(s) 1219
Cfr13I GGNCC 7 cut(s) 46, 54, 113, 579, 1903, 2033, 2397
Cfr9I CCCGGG 1 cut(s) 2292
Csp6I GTAC 2 cut(s) 1521, 2442
CspCI CAANNNNNGTGG 2 cut(s) 1840, 1875
CviQI GTAC 2 cut(s) 1521, 2442
DdeI CTNAG 6 cut(s) 861, 876, 1005, 1014, 1215, 1782
DpnI GATC 9 cut(s) 363, 540, 560, 852, 1146, 1470, 1517, 2133, 2376
DpnII GATC 9 cut(s) 361, 538, 558, 850, 1144, 1468, 1515, 2131, 2374
DraI TTTAAA 3 cut(s) 847, 1132, 2017
DriI GACNNNNNGTC 1 cut(s) 2514
EaeI YGGCCR 2 cut(s) 29, 1496
EagI CGGCCG 1 cut(s) 29
Eam1104I CTCTTC 2 cut(s) 204, 2292
Eam1105I GACNNNNNGTC 1 cut(s) 2514
EarI CTCTTC 2 cut(s) 204, 2292
Ecl136II GAGCTC 1 cut(s) 1011
EclXI CGGCCG 1 cut(s) 29
Eco130I CCWWGG 3 cut(s) 1098, 1731, 2400
Eco147I AGGCCT 1 cut(s) 91
Eco24I GRGCYC 1 cut(s) 1013
Eco32I GATATC 1 cut(s) 1184
Eco47I GGWCC 6 cut(s) 46, 54, 579, 1903, 2033, 2397
Eco52I CGGCCG 1 cut(s) 29
Eco53kI GAGCTC 1 cut(s) 1011
Eco57I CTGAAG 5 cut(s) 185, 357, 453, 876, 1280
Eco88I CYCGRG 1 cut(s) 2292
EcoICRI GAGCTC 1 cut(s) 1011
EcoO109I RGGNCCY 3 cut(s) 46, 579, 1903
EcoRI GAATTC 2 cut(s) 681, 913
EcoRII CCWGG 5 cut(s) 250, 670, 1207, 1341, 2035
EcoRV GATATC 1 cut(s) 1184
EcoT14I CCWWGG 3 cut(s) 1098, 1731, 2400
EcoT22I ATGCAT 1 cut(s) 618
EcoT38I GRGCYC 1 cut(s) 1013
ErhI CCWWGG 3 cut(s) 1098, 1731, 2400
FalI AAGNNNNNCTT 2 cut(s) 2361, 2393
FaqI GGGAC 1 cut(s) 565
FauI CCCGC 2 cut(s) 1634, 2333
FauNDI CATATG 1 cut(s) 1448
FblI GTMKAC 1 cut(s) 375
Fnu4HI GCNGC 7 cut(s) 29, 32, 342, 998, 1779, 2229, 2338
FokI GGATG 9 cut(s) 20, 89, 512, 791, 974, 1280, 1611, 2052, 2175
FriOI GRGCYC 1 cut(s) 1013
Fsp4HI GCNGC 7 cut(s) 29, 32, 342, 998, 1779, 2229, 2338
FspBI CTAG 9 cut(s) 87, 401, 455, 461, 513, 602, 1416, 1839, 1863
GlaI GCGC 1 cut(s) 285
GluI GCNGC 7 cut(s) 29, 32, 342, 998, 1779, 2229, 2338
GsuI CTGGAG 4 cut(s) 234, 378, 1656, 2383
HaeII RGCGCY 1 cut(s) 287
HaeIII GGCC 9 cut(s) 31, 91, 115, 833, 1207, 1312, 1498, 1709, 1944
HapII CCGG 4 cut(s) 1220, 1953, 2293, 2466
HhaI GCGC 1 cut(s) 286
Hin6I GCGC 1 cut(s) 284
HinP1I GCGC 1 cut(s) 284
HincII GTYRAC 4 cut(s) 354, 1405, 2046, 2353
HindII GTYRAC 4 cut(s) 354, 1405, 2046, 2353
HinfI GANTC 5 cut(s) 413, 715, 872, 896, 973
HpaII CCGG 4 cut(s) 1220, 1953, 2293, 2466
HphI GGTGA 6 cut(s) 23, 764, 1132, 1455, 2159, 2356
Hpy188I TCNGA 7 cut(s) 193, 370, 871, 895, 940, 972, 1299
HpyCH4III ACNGT 9 cut(s) 83, 595, 2087, 2171, 2260, 2350, 2446, 2493, 2512
HpyCH4IV ACGT 2 cut(s) 1636, 2074
HpyF10VI GCNNNNNNNGC 7 cut(s) 281, 338, 812, 1017, 1487, 2069, 2234
HpyF3I CTNAG 6 cut(s) 861, 876, 1005, 1014, 1215, 1782
HpySE526I ACGT 2 cut(s) 1636, 2074
HspAI GCGC 1 cut(s) 284
KflI GGGWCCC 1 cut(s) 579
KroI GCCGGC 1 cut(s) 1219
KroNI GCCGGC 1 cut(s) 1221
Kzo9I GATC 9 cut(s) 361, 538, 558, 850, 1144, 1468, 1515, 2131, 2374
LguI GCTCTTC 1 cut(s) 2292
LmnI GCTCC 7 cut(s) 164, 280, 517, 1279, 1508, 1696, 2364
Lsp1109I GCAGC 4 cut(s) 353, 1009, 1765, 2240
LweI GCATC 7 cut(s) 124, 427, 603, 625, 1613, 1795, 2197
MaeI CTAG 9 cut(s) 87, 401, 455, 461, 513, 602, 1416, 1839, 1863
MaeII ACGT 2 cut(s) 1636, 2074
MalI GATC 9 cut(s) 363, 540, 560, 852, 1146, 1470, 1517, 2133, 2376
MboI GATC 9 cut(s) 361, 538, 558, 850, 1144, 1468, 1515, 2131, 2374
MfeI CAATTG 2 cut(s) 144, 216
MflI RGATCY 7 cut(s) 361, 558, 850, 1144, 1515, 2131, 2374
MhlI GDGCHC 6 cut(s) 1013, 1276, 1680, 1891, 1917, 2074
MlsI TGGCCA 1 cut(s) 1498
MluNI TGGCCA 1 cut(s) 1498
MlyI GAGTC 1 cut(s) 905
MmeI TCCRAC 2 cut(s) 911, 1572
Mox20I TGGCCA 1 cut(s) 1498
Mph1103I ATGCAT 1 cut(s) 618
MroNI GCCGGC 1 cut(s) 1219
MroXI GAANNNNTTC 2 cut(s) 198, 1821
MscI TGGCCA 1 cut(s) 1498
MseI TTAA 7 cut(s) 846, 1131, 1290, 1380, 2016, 2319, 2495
MslI CAYNNNNRTG 5 cut(s) 633, 960, 1923, 2085, 2145
Msp20I TGGCCA 1 cut(s) 1498
MspA1I CMGCKG 1 cut(s) 344
MspI CCGG 4 cut(s) 1220, 1953, 2293, 2466
MspR9I CCNGG 8 cut(s) 252, 672, 1209, 1343, 1953, 2037, 2293, 2294
MunI CAATTG 2 cut(s) 144, 216
Mva1269I GAATGC 1 cut(s) 1343
MvaI CCWGG 5 cut(s) 252, 672, 1209, 1343, 2037
MvnI CGCG 1 cut(s) 2337
MwoI GCNNNNNNNGC 7 cut(s) 281, 338, 812, 1017, 1487, 2069, 2234
NaeI GCCGGC 1 cut(s) 1221
NciI CCSGG 3 cut(s) 1953, 2293, 2294
NcoI CCATGG 1 cut(s) 1731
NdeI CATATG 1 cut(s) 1448
NdeII GATC 9 cut(s) 361, 538, 558, 850, 1144, 1468, 1515, 2131, 2374
NgoMIV GCCGGC 1 cut(s) 1219
NlaIV GGNNCC 5 cut(s) 56, 580, 581, 1347, 1905
NmeAIII GCCGAG 1 cut(s) 366
NmuCI GTSAC 7 cut(s) 11, 550, 752, 1088, 1438, 1955, 2344
NotI GCGGCCGC 1 cut(s) 29
NsiI ATGCAT 1 cut(s) 618
NspI RCATGY 4 cut(s) 773, 1684, 1928, 2096
NspV TTCGAA 1 cut(s) 530
OliI CACNNNNGTG 1 cut(s) 2085
PceI AGGCCT 1 cut(s) 91
PciI ACATGT 2 cut(s) 1680, 1924
PciSI GCTCTTC 1 cut(s) 2292
PctI GAATGC 1 cut(s) 1343
PdiI GCCGGC 1 cut(s) 1221
PdmI GAANNNNTTC 2 cut(s) 198, 1821
PfeI GAWTC 4 cut(s) 413, 715, 872, 973
PfoI TCCNGGA 1 cut(s) 2035
PkrI GCNGC 7 cut(s) 30, 33, 343, 999, 1780, 2230, 2339
PleI GAGTC 1 cut(s) 904
PpsI GAGTC 1 cut(s) 904
PpuMI RGGWCCY 3 cut(s) 46, 579, 1903
PscI ACATGT 2 cut(s) 1680, 1924
PshBI ATTAAT 1 cut(s) 1290
Psp124BI GAGCTC 1 cut(s) 1013
Psp5II RGGWCCY 3 cut(s) 46, 579, 1903
Psp6I CCWGG 5 cut(s) 250, 670, 1207, 1341, 2035
PspGI CCWGG 5 cut(s) 250, 670, 1207, 1341, 2035
PspN4I GGNNCC 5 cut(s) 56, 580, 581, 1347, 1905
PspPI GGNCC 7 cut(s) 46, 54, 113, 579, 1903, 2033, 2397
PspPPI RGGWCCY 3 cut(s) 46, 579, 1903
PstI CTGCAG 3 cut(s) 40, 730, 1233
PsuI RGATCY 7 cut(s) 361, 558, 850, 1144, 1515, 2131, 2374
RsaI GTAC 2 cut(s) 1522, 2443
RsaNI GTAC 2 cut(s) 1521, 2442
RseI CAYNNNNRTG 5 cut(s) 633, 960, 1923, 2085, 2145
SacI GAGCTC 1 cut(s) 1013
SapI GCTCTTC 1 cut(s) 2292
SaqAI TTAA 7 cut(s) 846, 1131, 1290, 1380, 2016, 2319, 2495
SatI GCNGC 7 cut(s) 29, 32, 342, 998, 1779, 2229, 2338
Sau3AI GATC 9 cut(s) 361, 538, 558, 850, 1144, 1468, 1515, 2131, 2374
Sau96I GGNCC 7 cut(s) 46, 54, 113, 579, 1903, 2033, 2397
SbfI CCTGCAGG 1 cut(s) 1233
SchI GAGTC 1 cut(s) 905
ScrFI CCNGG 8 cut(s) 252, 672, 1209, 1343, 1953, 2037, 2293, 2294
SdaI CCTGCAGG 1 cut(s) 1233
SduI GDGCHC 6 cut(s) 1013, 1276, 1680, 1891, 1917, 2074
SfaNI GCATC 7 cut(s) 124, 427, 603, 625, 1613, 1795, 2197
SfcI CTRYAG 3 cut(s) 36, 726, 1229
SfuI TTCGAA 1 cut(s) 530
SinI GGWCC 6 cut(s) 46, 54, 579, 1903, 2033, 2397
SmaI CCCGGG 1 cut(s) 2294
SmiMI CAYNNNNRTG 5 cut(s) 633, 960, 1923, 2085, 2145
SmlI CTYRAG 2 cut(s) 422, 1800
SmoI CTYRAG 2 cut(s) 422, 1800
Sse8387I CCTGCAGG 1 cut(s) 1233
SseBI AGGCCT 1 cut(s) 91
SsiI CCGC 6 cut(s) 28, 32, 344, 1641, 2337, 2340
SspI AATATT 1 cut(s) 488
SspMI CTAG 9 cut(s) 87, 401, 455, 461, 513, 602, 1416, 1839, 1863
SstI GAGCTC 1 cut(s) 1013
StuI AGGCCT 1 cut(s) 91
StyD4I CCNGG 8 cut(s) 250, 670, 1207, 1341, 1951, 2035, 2291, 2292
StyI CCWWGG 3 cut(s) 1098, 1731, 2400
TaaI ACNGT 9 cut(s) 83, 595, 2087, 2171, 2260, 2350, 2446, 2493, 2512
TaiI ACGT 2 cut(s) 1639, 2077
TaqI TCGA 4 cut(s) 530, 1180, 1471, 1921
TatI WGTACW 2 cut(s) 1520, 2441
TauI GCSGC 3 cut(s) 31, 34, 2340
TfiI GAWTC 4 cut(s) 413, 715, 872, 973
Tru1I TTAA 7 cut(s) 846, 1131, 1290, 1380, 2016, 2319, 2495
Tru9I TTAA 7 cut(s) 846, 1131, 1290, 1380, 2016, 2319, 2495
TscAI CASTG 6 cut(s) 910, 1717, 2004, 2092, 2188, 2263
TseFI GTSAC 7 cut(s) 11, 550, 752, 1088, 1438, 1955, 2344
TseI GCWGC 4 cut(s) 341, 997, 1778, 2228
Tsp45I GTSAC 7 cut(s) 11, 550, 752, 1088, 1438, 1955, 2344
TspDTI ATGAA 9 cut(s) 714, 831, 971, 1061, 1155, 1185, 1316, 1421, 2157
TspGWI ACGGA 1 cut(s) 1398
TspMI CCCGGG 1 cut(s) 2292
TspRI CASTG 6 cut(s) 910, 1717, 2004, 2092, 2188, 2263
VneI GTGCAC 1 cut(s) 1913
VpaK11BI GGWCC 6 cut(s) 46, 54, 579, 1903, 2033, 2397
VspI ATTAAT 1 cut(s) 1290
XapI RAATTY 7 cut(s) 404, 681, 702, 788, 913, 925, 1075
XbaI TCTAGA 1 cut(s) 601
XceI RCATGY 4 cut(s) 773, 1684, 1928, 2096
XmaI CCCGGG 1 cut(s) 2292
XmiI GTMKAC 1 cut(s) 375
XmnI GAANNNNTTC 2 cut(s) 198, 1821
XspI CTAG 9 cut(s) 87, 401, 455, 461, 513, 602, 1416, 1839, 1863
Zsp2I ATGCAT 1 cut(s) 618
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.