Rw6G006150

alpha-L-fucosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0004443
Physical Location & Seq
Reverse (-)
9766236 .. 9772888
6653 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G006150.1

Sequence Viewer

Length: 1611 bp
ATGCATCATGGTTCACATTTAAATGGTAAAAACCAAATTATACTTGAAGGAAGTTGTCCTGGTAGAAGAATTTCACCAAAGTATAATGACAATCCAGAGGGGATTCAGTTTACTGCAGTTCTTGATTTGCAGATTAGTGGTGACAAAGGTGTGATACATGCTTTGGATGACAAGAAATTTAGAGTTGAAAAGGCAGATTGGGCTGTTTTGCTTCTGGTGGCCTCATCTTCATTTAAGGATCCATTTACTAAGCCTTCTGATTCTAAGAGAAATCCAACTTCAGAGTCTCTCACTGCATTGAATTCAATAAGAAATTTGTCATATTCTGACCTCTATGCTCATCATTTGGATGACTATCAGAATCTTTTTCATCGTGTCTCATTGCAGCTCTCTAAGAGCTCTAAGAGCATGTTAGGAAATGAAACTTTGGAGATGAAGAAGCTCAACCCTATTACCAATTTGAATTTCAAGGGAAGTGACAATGCCTTGGGTTCTACTGCTGACAGGGTGAAATCCTTTAAAACTGATGAAGATCCTTCTTTTGTGGAACTCTTGTTCCAATATGGTCGATATCTCCTTATTTCATGCTCACGGCCTGGAACTCAGCCGGCAAACCTGCAGGGTATATGGAACTACAAAATTGAACCTGCATGGGATGGTGCTCCTCATACGAACATTAATCTTCAGATGAACTATTGGCCTTCCCTTCCTTGTAATCTACGAGAATGCCAGGAACCCTTATTTGATTTCACATCTTTATTATCTATTAACGGGAGTAAAACAGCAAAGGTCAACTATGAAGTTAGTGGTTGGGTTGTGCATCAAGTGACTGACATATGGGCGAAAACATCACCGGATCGAGGTCAAGCTGTATGGGCTTTATGGCCAATGGGAGGGGCGTGGATCTGTACTCATCTATGGGAACATTATACTTATACAATGGACAAGGATTTCCTGAAAAATAAGGCATATCCTTTGTTGGAAGGATGTGCATCATTTCTGCTGGACTGGTTGATTGAAGGACGTGGTGGGTATCTTGAAACCAACCCATCAACTTCTCCAGAGCACATGTTTATTGCTCCTGATGGTAAGCAGGCCAGCGTGAGCTACTCATCAACCATGGACATCTCAATCATAAAAGAAGTCTTTTCTGCAGTACTTTCTGCTGCTGAGGTTCTTGGAAAAACTCAAGATACTTTTGTTGTAAAAGTTCGTAATGCTCAATCTAGGCTTCCACCCACAAAAATTGCTAGAGATGGTTCTATTATGGAATGGGCACAAGATTTTGAGGACCCAGAAGTGCACCATCGACATGTATCACACCTGTTTGGCCTGTTTCCGGGCCACACAATAACTCTTGAAAAAACTCCAGACCTCTGTAAAGCAGTGGATTATACCCTTTTTAAAAGAGGAGAGGAAGGTCCAGGATGGTCAACTACCTGGAAGACTGCATTGTGGGCACGTCTTCACAACAGTGAACATGCGTATCGTATGGTCAAGCATTTGATTGACTTGGTAGATCCAGAACGTGAAGGTGATTTTGAAGGAGGACTATACAGTAACTTGTTCACTGCACATCCTCCTTTCCAGATTGATGCCAACTTTGGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004553 GO:0004560 GO:0005575 GO:0005576 GO:0015928 GO:0016787 GO:0016798 GO:0047513 GO:0048046
KEGG Pathways
Metabolic & Signaling
ko00511 map00511
Pfam Domains
Protein Families
Glyco_hyd_65N_2

Protein Analysis

536

Amino Acids

60.41

Weight (kDa)

6.09

Isoelectric Point (pI)

30.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_95_cat PF22124 107 - 536 2e-163 Glycosyl hydrolase family 95 catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 624, 655
AclWI GGATC 6 cut(s) 233, 246, 527, 864, 911, 1514
AcoI YGGCCR 1 cut(s) 884
AcsI RAATTY 5 cut(s) 69, 176, 301, 313, 463
AcuI CTGAAG 2 cut(s) 264, 668
AfaI GTAC 2 cut(s) 910, 1158
AfiI CCNNNNNNNGG 4 cut(s) 860, 893, 1339, 1605
AflIII ACRYGT 2 cut(s) 1068, 1312
AjiI CACGTC 2 cut(s) 1025, 1463
AjnI CCWGG 5 cut(s) 58, 595, 729, 1423, 1439
AjuI GAANNNNNNNTTGG 2 cut(s) 449, 481
AleI CACNNNNGTG 1 cut(s) 1473
AloI GAACNNNNNNTCC 4 cut(s) 539, 540, 571, 572
AluBI AGCT 5 cut(s) 388, 399, 442, 869, 1107
AluI AGCT 5 cut(s) 388, 399, 442, 869, 1107
Alw21I GWGCWC 4 cut(s) 401, 664, 1068, 1305
Alw26I GTCTC 2 cut(s) 291, 382
Alw44I GTGCAC 1 cut(s) 1301
AlwI GGATC 6 cut(s) 233, 246, 527, 864, 911, 1514
AoxI GGCC 7 cut(s) 219, 593, 698, 884, 1095, 1330, 1342
ApaLI GTGCAC 1 cut(s) 1301
ApeKI GCWGC 2 cut(s) 385, 1166
ApoI RAATTY 5 cut(s) 69, 176, 301, 313, 463
AseI ATTAAT 1 cut(s) 678
Asp700I GAANNNNTTC 1 cut(s) 70
AspS9I GGNCC 3 cut(s) 1291, 1342, 1421
AsuC2I CCSGG 1 cut(s) 1341
AsuHPI GGTGA 5 cut(s) 66, 152, 520, 843, 1547
AvaII GGWCC 2 cut(s) 1291, 1421
BaeGI GKGCMC 3 cut(s) 1279, 1305, 1462
BaeI ACNNNNGTAYC 4 cut(s) 1185, 1218, 1469, 1502
BalI TGGCCA 1 cut(s) 886
BamHI GGATCC 1 cut(s) 238
BanII GRGCYC 1 cut(s) 401
BbsI GAAGAC 2 cut(s) 1451, 1457
Bbv12I GWGCWC 4 cut(s) 401, 664, 1068, 1305
BbvCI CCTCAGC 1 cut(s) 1170
BbvI GCAGC 2 cut(s) 397, 1153
BccI CCATC 6 cut(s) 650, 1057, 1079, 1250, 1314, 1422
BceAI ACGGC 1 cut(s) 608
BciT130I CCWGG 5 cut(s) 60, 597, 731, 1425, 1441
BcnI CCSGG 1 cut(s) 1341
BcoDI GTCTC 2 cut(s) 291, 382
BfaI CTAG 2 cut(s) 1227, 1251
BfmI CTRYAG 3 cut(s) 114, 617, 1152
BfuAI ACCTGC 2 cut(s) 624, 655
BisI GCNGC 2 cut(s) 386, 1167
BlsI GCNGC 2 cut(s) 387, 1168
BmcAI AGTACT 1 cut(s) 1158
Bme1390I CCNGG 6 cut(s) 60, 597, 731, 1341, 1425, 1441
Bme18I GGWCC 2 cut(s) 1291, 1421
BmgBI CACGTC 2 cut(s) 1025, 1463
BmgT120I GGNCC 3 cut(s) 1291, 1342, 1421
BmiI GGNNCC 3 cut(s) 240, 735, 1293
BmrFI CCNGG 6 cut(s) 60, 597, 731, 1341, 1425, 1441
BmsI GCATC 4 cut(s) 13, 829, 1001, 1585
BpiI GAAGAC 2 cut(s) 1451, 1457
BpmI CTGGAG 2 cut(s) 1044, 1353
Bpu10I CCTNAGC 1 cut(s) 1170
BpuEI CTTGAG 1 cut(s) 1173
BpuMI CCSGG 1 cut(s) 1341
BsaBI GATNNNNATC 3 cut(s) 354, 531, 991
BsaJI CCNNGG 2 cut(s) 486, 1119
BsaWI WCCGGW 1 cut(s) 853
Bsc4I CCNNNNNNNGG 4 cut(s) 860, 893, 1339, 1605
Bse118I RCCGGY 1 cut(s) 607
Bse1I ACTGG 1 cut(s) 1013
Bse3DI GCAATG 1 cut(s) 380
Bse8I GATNNNNATC 3 cut(s) 354, 531, 991
BseBI CCWGG 5 cut(s) 60, 597, 731, 1425, 1441
BseDI CCNNGG 2 cut(s) 486, 1119
BseGI GGATG 6 cut(s) 172, 355, 661, 992, 1433, 1576
BseJI GATNNNNATC 3 cut(s) 354, 531, 991
BseLI CCNNNNNNNGG 4 cut(s) 860, 893, 1339, 1605
BseMI GCAATG 1 cut(s) 380
BseMII CTCAG 2 cut(s) 617, 1161
BseNI ACTGG 1 cut(s) 1013
BseRI GAGGAG 2 cut(s) 654, 1425
BseSI GKGCMC 3 cut(s) 1279, 1305, 1462
BseXI GCAGC 2 cut(s) 397, 1153
BsgI GTGCAG 1 cut(s) 1557
BshFI GGCC 7 cut(s) 221, 595, 700, 886, 1097, 1332, 1344
BsiHKAI GWGCWC 4 cut(s) 401, 664, 1068, 1305
BsiSI CCGG 3 cut(s) 608, 854, 1340
BslI CCNNNNNNNGG 4 cut(s) 860, 893, 1339, 1605
BsmAI GTCTC 2 cut(s) 291, 382
BsmI GAATGC 1 cut(s) 731
BsnI GGCC 7 cut(s) 221, 595, 700, 886, 1097, 1332, 1344
Bsp1286I GDGCHC 6 cut(s) 401, 664, 1068, 1279, 1305, 1462
Bsp143I GATC 5 cut(s) 238, 532, 856, 903, 1519
Bsp19I CCATGG 1 cut(s) 1119
BspANI GGCC 7 cut(s) 221, 595, 700, 886, 1097, 1332, 1344
BspCNI CTCAG 2 cut(s) 616, 1162
BspLI GGNNCC 3 cut(s) 240, 735, 1293
BspMAI CTGCAG 3 cut(s) 118, 621, 1156
BspMI ACCTGC 2 cut(s) 624, 655
BspPI GGATC 6 cut(s) 233, 246, 527, 864, 911, 1514
BsrDI GCAATG 1 cut(s) 380
BsrFI RCCGGY 1 cut(s) 607
BsrI ACTGG 1 cut(s) 1013
BssAI RCCGGY 1 cut(s) 607
BssECI CCNNGG 2 cut(s) 486, 1119
BssMI GATC 5 cut(s) 238, 532, 856, 903, 1519
BssT1I CCWWGG 2 cut(s) 486, 1119
Bst2UI CCWGG 5 cut(s) 60, 597, 731, 1425, 1441
Bst4CI ACNGT 2 cut(s) 1475, 1559
BstC8I GCNNGC 3 cut(s) 609, 1095, 1099
BstDEI CTNAG 6 cut(s) 249, 264, 393, 402, 603, 1170
BstDSI CCRYGG 1 cut(s) 1119
BstF5I GGATG 6 cut(s) 172, 355, 661, 992, 1433, 1576
BstKTI GATC 5 cut(s) 241, 535, 859, 906, 1522
BstMAI GTCTC 2 cut(s) 291, 382
BstMBI GATC 5 cut(s) 238, 532, 856, 903, 1519
BstMWI GCNNNNNNNGC 4 cut(s) 200, 405, 875, 1457
BstNI CCWGG 5 cut(s) 60, 597, 731, 1425, 1441
BstNSI RCATGY 5 cut(s) 161, 412, 1072, 1316, 1484
BstSCI CCNGG 6 cut(s) 58, 595, 729, 1339, 1423, 1439
BstSFI CTRYAG 3 cut(s) 114, 617, 1152
BstSLI GKGCMC 3 cut(s) 1279, 1305, 1462
BstV1I GCAGC 2 cut(s) 397, 1153
BstV2I GAAGAC 2 cut(s) 1451, 1457
BstX2I RGATCY 4 cut(s) 238, 532, 903, 1519
BstYI RGATCY 4 cut(s) 238, 532, 903, 1519
BsuRI GGCC 7 cut(s) 221, 595, 700, 886, 1097, 1332, 1344
BtgI CCRYGG 1 cut(s) 1119
BtrI CACGTC 2 cut(s) 1025, 1463
BtsCI GGATG 6 cut(s) 172, 355, 661, 992, 1433, 1576
BtsI GCAGTG 3 cut(s) 291, 1392, 1569
BtsIMutI CAGTG 4 cut(s) 291, 1392, 1480, 1569
BveI ACCTGC 2 cut(s) 624, 655
Cac8I GCNNGC 3 cut(s) 609, 1095, 1099
Cfr10I RCCGGY 1 cut(s) 607
Cfr13I GGNCC 3 cut(s) 1291, 1342, 1421
Csp6I GTAC 2 cut(s) 909, 1157
CspCI CAANNNNNGTGG 2 cut(s) 1228, 1263
CviAII CATG 9 cut(s) 8, 158, 409, 585, 651, 1069, 1120, 1313, 1481
CviQI GTAC 2 cut(s) 909, 1157
DdeI CTNAG 6 cut(s) 249, 264, 393, 402, 603, 1170
DpnI GATC 5 cut(s) 240, 534, 858, 905, 1521
DpnII GATC 5 cut(s) 238, 532, 856, 903, 1519
DraI TTTAAA 3 cut(s) 21, 520, 1405
EaeI YGGCCR 1 cut(s) 884
Ecl136II GAGCTC 1 cut(s) 399
Eco130I CCWWGG 2 cut(s) 486, 1119
Eco24I GRGCYC 1 cut(s) 401
Eco32I GATATC 1 cut(s) 572
Eco47I GGWCC 2 cut(s) 1291, 1421
Eco53kI GAGCTC 1 cut(s) 399
Eco57I CTGAAG 2 cut(s) 264, 668
EcoICRI GAGCTC 1 cut(s) 399
EcoO109I RGGNCCY 1 cut(s) 1291
EcoRI GAATTC 1 cut(s) 301
EcoRII CCWGG 5 cut(s) 58, 595, 729, 1423, 1439
EcoRV GATATC 1 cut(s) 572
EcoT14I CCWWGG 2 cut(s) 486, 1119
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 1 cut(s) 401
ErhI CCWWGG 2 cut(s) 486, 1119
FaeI CATG 9 cut(s) 11, 161, 412, 588, 654, 1072, 1123, 1316, 1484
FatI CATG 9 cut(s) 7, 157, 408, 584, 650, 1068, 1119, 1312, 1480
FauNDI CATATG 1 cut(s) 836
Fnu4HI GCNGC 2 cut(s) 386, 1167
FokI GGATG 6 cut(s) 179, 362, 668, 999, 1440, 1563
FriOI GRGCYC 1 cut(s) 401
Fsp4HI GCNGC 2 cut(s) 386, 1167
FspBI CTAG 2 cut(s) 1227, 1251
GluI GCNGC 2 cut(s) 386, 1167
GsuI CTGGAG 2 cut(s) 1044, 1353
HaeIII GGCC 7 cut(s) 221, 595, 700, 886, 1097, 1332, 1344
HapII CCGG 3 cut(s) 608, 854, 1340
Hin1II CATG 9 cut(s) 11, 161, 412, 588, 654, 1072, 1123, 1316, 1484
HincII GTYRAC 2 cut(s) 793, 1434
HindII GTYRAC 2 cut(s) 793, 1434
HinfI GANTC 4 cut(s) 103, 260, 284, 361
HpaII CCGG 3 cut(s) 608, 854, 1340
HphI GGTGA 5 cut(s) 66, 152, 520, 843, 1547
Hpy166II GTNNAC 7 cut(s) 14, 111, 793, 1303, 1434, 1478, 1569
Hpy188I TCNGA 5 cut(s) 259, 283, 328, 360, 687
Hpy8I GTNNAC 7 cut(s) 14, 111, 793, 1303, 1434, 1478, 1569
HpyCH4III ACNGT 2 cut(s) 1475, 1559
HpyCH4IV ACGT 3 cut(s) 1024, 1462, 1528
HpyF10VI GCNNNNNNNGC 4 cut(s) 200, 405, 875, 1457
HpyF3I CTNAG 6 cut(s) 249, 264, 393, 402, 603, 1170
HpySE526I ACGT 3 cut(s) 1024, 1462, 1528
Hsp92II CATG 9 cut(s) 11, 161, 412, 588, 654, 1072, 1123, 1316, 1484
KroI GCCGGC 1 cut(s) 607
KroNI GCCGGC 1 cut(s) 609
Kzo9I GATC 5 cut(s) 238, 532, 856, 903, 1519
LmnI GCTCC 2 cut(s) 667, 1084
Lsp1109I GCAGC 2 cut(s) 397, 1153
LweI GCATC 4 cut(s) 13, 829, 1001, 1585
MaeI CTAG 2 cut(s) 1227, 1251
MaeII ACGT 3 cut(s) 1024, 1462, 1528
MaeIII GTNAC 4 cut(s) 140, 476, 826, 1559
MalI GATC 5 cut(s) 240, 534, 858, 905, 1521
MboI GATC 5 cut(s) 238, 532, 856, 903, 1519
MboII GAAGA 7 cut(s) 78, 219, 448, 542, 674, 1456, 1457
MflI RGATCY 4 cut(s) 238, 532, 903, 1519
MhlI GDGCHC 6 cut(s) 401, 664, 1068, 1279, 1305, 1462
MlsI TGGCCA 1 cut(s) 886
MluCI AATT 9 cut(s) 36, 69, 176, 301, 313, 457, 463, 639, 1245
MluNI TGGCCA 1 cut(s) 886
MlyI GAGTC 1 cut(s) 293
MmeI TCCRAC 2 cut(s) 299, 960
Mox20I TGGCCA 1 cut(s) 886
Mph1103I ATGCAT 1 cut(s) 6
MroNI GCCGGC 1 cut(s) 607
MroXI GAANNNNTTC 1 cut(s) 70
MscI TGGCCA 1 cut(s) 886
MseI TTAA 6 cut(s) 20, 234, 519, 678, 768, 1404
MslI CAYNNNNRTG 4 cut(s) 21, 348, 1311, 1473
Msp20I TGGCCA 1 cut(s) 886
MspI CCGG 3 cut(s) 608, 854, 1340
MspR9I CCNGG 6 cut(s) 60, 597, 731, 1341, 1425, 1441
Mva1269I GAATGC 1 cut(s) 731
MvaI CCWGG 5 cut(s) 60, 597, 731, 1425, 1441
MwoI GCNNNNNNNGC 4 cut(s) 200, 405, 875, 1457
NaeI GCCGGC 1 cut(s) 609
NciI CCSGG 1 cut(s) 1341
NcoI CCATGG 1 cut(s) 1119
NdeI CATATG 1 cut(s) 836
NdeII GATC 5 cut(s) 238, 532, 856, 903, 1519
NgoMIV GCCGGC 1 cut(s) 607
NlaIII CATG 9 cut(s) 11, 161, 412, 588, 654, 1072, 1123, 1316, 1484
NlaIV GGNNCC 3 cut(s) 240, 735, 1293
NmuCI GTSAC 3 cut(s) 140, 476, 826
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 5 cut(s) 161, 412, 1072, 1316, 1484
OliI CACNNNNGTG 1 cut(s) 1473
PciI ACATGT 2 cut(s) 1068, 1312
PctI GAATGC 1 cut(s) 731
PdiI GCCGGC 1 cut(s) 609
PdmI GAANNNNTTC 1 cut(s) 70
PfeI GAWTC 3 cut(s) 103, 260, 361
PfoI TCCNGGA 1 cut(s) 1423
PkrI GCNGC 2 cut(s) 387, 1168
PleI GAGTC 1 cut(s) 292
PpsI GAGTC 1 cut(s) 292
PpuMI RGGWCCY 1 cut(s) 1291
PscI ACATGT 2 cut(s) 1068, 1312
PshBI ATTAAT 1 cut(s) 678
Psp124BI GAGCTC 1 cut(s) 401
Psp5II RGGWCCY 1 cut(s) 1291
Psp6I CCWGG 5 cut(s) 58, 595, 729, 1423, 1439
PspGI CCWGG 5 cut(s) 58, 595, 729, 1423, 1439
PspN4I GGNNCC 3 cut(s) 240, 735, 1293
PspPI GGNCC 3 cut(s) 1291, 1342, 1421
PspPPI RGGWCCY 1 cut(s) 1291
PstI CTGCAG 3 cut(s) 118, 621, 1156
PsuI RGATCY 4 cut(s) 238, 532, 903, 1519
RsaI GTAC 2 cut(s) 910, 1158
RsaNI GTAC 2 cut(s) 909, 1157
RseI CAYNNNNRTG 4 cut(s) 21, 348, 1311, 1473
SacI GAGCTC 1 cut(s) 401
SaqAI TTAA 6 cut(s) 20, 234, 519, 678, 768, 1404
SatI GCNGC 2 cut(s) 386, 1167
Sau3AI GATC 5 cut(s) 238, 532, 856, 903, 1519
Sau96I GGNCC 3 cut(s) 1291, 1342, 1421
SbfI CCTGCAGG 1 cut(s) 621
ScaI AGTACT 1 cut(s) 1158
SchI GAGTC 1 cut(s) 293
ScrFI CCNGG 6 cut(s) 60, 597, 731, 1341, 1425, 1441
SdaI CCTGCAGG 1 cut(s) 621
SduI GDGCHC 6 cut(s) 401, 664, 1068, 1279, 1305, 1462
SfaNI GCATC 4 cut(s) 13, 829, 1001, 1585
SfcI CTRYAG 3 cut(s) 114, 617, 1152
SinI GGWCC 2 cut(s) 1291, 1421
SmiI ATTTAAAT 1 cut(s) 21
SmiMI CAYNNNNRTG 4 cut(s) 21, 348, 1311, 1473
SmlI CTYRAG 1 cut(s) 1188
SmoI CTYRAG 1 cut(s) 1188
Sse8387I CCTGCAGG 1 cut(s) 621
Sse9I AATT 9 cut(s) 36, 69, 176, 301, 313, 457, 463, 639, 1245
SspMI CTAG 2 cut(s) 1227, 1251
SstI GAGCTC 1 cut(s) 401
StyD4I CCNGG 6 cut(s) 58, 595, 729, 1339, 1423, 1439
StyI CCWWGG 2 cut(s) 486, 1119
SwaI ATTTAAAT 1 cut(s) 21
TaaI ACNGT 2 cut(s) 1475, 1559
TaiI ACGT 3 cut(s) 1027, 1465, 1531
TaqI TCGA 3 cut(s) 568, 859, 1309
TasI AATT 9 cut(s) 36, 69, 176, 301, 313, 457, 463, 639, 1245
TatI WGTACW 2 cut(s) 908, 1156
TfiI GAWTC 3 cut(s) 103, 260, 361
Tru1I TTAA 6 cut(s) 20, 234, 519, 678, 768, 1404
Tru9I TTAA 6 cut(s) 20, 234, 519, 678, 768, 1404
TscAI CASTG 4 cut(s) 298, 1392, 1480, 1576
TseFI GTSAC 3 cut(s) 140, 476, 826
TseI GCWGC 2 cut(s) 385, 1166
Tsp45I GTSAC 3 cut(s) 140, 476, 826
TspDTI ATGAA 8 cut(s) 219, 359, 435, 449, 543, 573, 704, 813
TspRI CASTG 4 cut(s) 298, 1392, 1480, 1576
VneI GTGCAC 1 cut(s) 1301
VpaK11BI GGWCC 2 cut(s) 1291, 1421
VspI ATTAAT 1 cut(s) 678
XapI RAATTY 5 cut(s) 69, 176, 301, 313, 463
XceI RCATGY 5 cut(s) 161, 412, 1072, 1316, 1484
XmnI GAANNNNTTC 1 cut(s) 70
XspI CTAG 2 cut(s) 1227, 1251
ZrmI AGTACT 1 cut(s) 1158
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.