FvH4_2g04870

alpha-L-fucosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0004443
Physical Location & Seq
Reverse (-)
3781577 .. 3786880
5304 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g04870.t1

Sequence Viewer

Length: 2628 bp
ATGGAGGACGGTGACTGGGTTCTGGTGCGGCCGCCTGCAGAGAAGGACCTCTGGACTCCTAGTTTAGTGAAGGAACATGAGAGTTCTGAGCCTTTGAAGGTTACTTTTGGGGGGCCTGCAAAGTTCTGGACTGATGCAATTCCAATTGGCAATGGGAGGCTTGGAGCTATGGTCTGGGGTGGTGTGGCTTCAGAAACTCTTCAGCTCAATGAGGACACACTCTGGACTGGAACTCCGGGCAACTACACCAACCCGAATGCTCCACAGGCGCTAACAGAGGTTAGGAAACTTGTTGATGATGGTAAATATGCTGAAGCAACTGAAGCAGCGGTCAAGTTGAGTGGAGAGCAATCTGCTGTATACCAACTACTCTGCGACATCAACCTGGAGTTTGATGATTCACATCTCAAGTATGCTGAAGAAACTTACAGCAGAGAATTAGACCTAGATACTGCAACAGCAAGGATTAAATATTCTGTAGGAGAGGTAGAATATACTAGGGAGCATTTTGCTTCGAATCCTGACCAAGTGATTGTGACAAAGATATCTGCAAGCAAATCAGGATCCCTATCATTTACAGTTTCTCTTGACAGCAAGTTGCATCATGGTTCACATGTAAATGGTCTAAACCAAATTATACTTGAAGGAAGTTGTCCTGGTAAAAGAATCGATGACGGGATTCAGTTTACTGCAGTTCTTGATTTGCAGATTAGTGGTGACAAAGGTGTGATACATGCTTTGGATGACATGAAATTTAGAGTTGAAGAGGCAGATTGGGCTGTTTTGCTTCTGGTGGCCTCATCTTCATTTAAAAATCCATTTACTGAGCCTTCTGATTCTAAGAGAAGTCCGACTTCAGAGTCTCTCGCTGCATTGAATTCAATAAAAAATTTATCATATTCTGACCTCTATGCTCATCATTTGGATGACTATCAGAATCTTTTTCATCGTGTCTCATTGCAGCTTTCTAAAAGCTGTAAGAGCATATATGAAAATAAAAGTTTGGAGATGAAGAAGTACTACCCCATTAAAAATTTGAATTTTAAGGGAAGTGACAATGCCTTGGGCTCTACTGCTGACAGGGTGAATTCCTTTAAAACTGATGAAGATCCTTCACTTGTGGAACTCTTGTTCCAATATGGTCGATATCTTCTTATTTCCTGTTCACGGCCTGGAACTCAACCTGCAAACCTGCAGGGTATATGGAACGACAAAACCTCACCTCCATGGGATGGTGCTCCTCATACGAACATTAATCTTCAGATGAACTATTGGCCTTCGCTTCCTTGTAATCTACGAGAATGCCAAGAACCCTTATTTGATTACACATCTTCACTATCTATTAATGGAAGTAAAACCGCAGAGGTCAACTATGAAGCTAGTGGTTGGGTTGTGCATCAAGTGACTGACGTTTGGGCAAAAACATCACCAGATCGAGGTGAAGCTGTATGGGCTTTATGGCCTATGGGGGGAGCGTGGCTCTGTACTCATCTATGGGAACATTATACTTATACAATGGATAAAGCGAGCTCCTATTCTCTCAAAACATTGTCTGTTTTCTATTTCTTGGAGAGAGAGAGGGGTTATGTTTTAGGTGCCAGCACTCCAACTACATGGTTGGATTGGGAGTCTAATTTCATTACAACTTACCAGTTAAAGTCAATTGATTTCCTGAAAAGTAAGGCATATCCTTTGTTGGAAGGATGTGCATCATTTCTTCTGGACTGGTTGATTGAAGGACCTGGCGAGTATCTTGAAACCAACCCATCAACTTCTCCAGAGCACATGTTTATCGCTCCTGATGGTAAGCAGGCCAGTGTGAGCTATTCAACAACCATGGACATCTCAATCATTAAAGAAGTCTTTTCCGCAGTACTTTCTGCTGCTGAGGTTTTAGGAAAAACTCAAGATACTTTTATTGAACATGTTCGCAATGCTCAATCAAGGCTTCCACCCACAAAAATTGCTAGAGATGGTTCTATTATGGAATGGGCACAAGATTTTGAGGACCCAGATGTGCACCATCGACATGTATCACACTTGTTTGGCCTGTTTCCAGGCCACACAATAACTCTTGAAAAAAATCCAGACCTCTGTAAAGCAGTGGATTATACCCTTTATAAAAGAGGAGAGGAAGGTCCAGGATGGTCTACGACATGGAAGACTGCATTGTGGGCACGTCTTCACAACAGTGAACATGCATATCGTATGGTAAAGCATTTGATTAACTTGGTGGATCCAGAGCGTGAAAGTGATTTTGAAGGAGGACTATACAGTAACTTGTTCACTGCACATCCTCCTTTCCAGATTGATGCCAACTTTGGTTTTTCAGCAGCAGTTGCCGAAATGCTTGTCCAAAGCACGGTTGAGGACTTGTATTTACTTCCTGCACTTCCCCGGGATAAATGGGCAAATGGTTGTATCAAAGGCTTGAAGGCACGCGGCAGGGTGACAGTCAACATCTGCTGGAGTGAAGGAGATCTTCACGAAGTTGGCATTTGGTCGGAGAACAACAACACTGTTAAAGTACTACATTATAGAGGAAGTAAAGTTACGGCAAACATATCGTCCGGCAGGATTTATACTTTCAACAGACAGTTAAAATGTGTGAGGACAGTTACTCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004553 GO:0004560 GO:0005575 GO:0005576 GO:0015928 GO:0016787 GO:0016798 GO:0047513 GO:0048046
KEGG Pathways
Metabolic & Signaling
ko00511 map00511
Pfam Domains
Protein Families
Glyco_hyd_65N_2

Protein Analysis

876

Amino Acids

98.04

Weight (kDa)

5.38

Isoelectric Point (pI)

28.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hyd_65N_2 PF14498 36 - 272 1.9e-62 Glycosyl hydrolase family 65, N-terminal domain
Glyco_hydro_95_cat PF22124 299 - 509 7.7e-75 Glycosyl hydrolase family 95 catalytic domain
Glyco_hydro_95_cat PF22124 554 - 784 9.8e-85 Glycosyl hydrolase family 95 catalytic domain
Glyco_hydro_95_C PF21307 786 - 853 3e-14 Glycoside hydrolase family 95, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2121
AasI GACNNNNNNGTC 1 cut(s) 859
Acc36I ACCTGC 2 cut(s) 1192, 1200
AccB1I GGYRCC 1 cut(s) 1595
AccB7I CCANNNNNTGG 1 cut(s) 1232
AccI GTMKAC 2 cut(s) 360, 2150
AccII CGCG 1 cut(s) 2442
AciI CCGC 6 cut(s) 28, 32, 329, 1359, 1869, 2442
AclWI GGATC 5 cut(s) 558, 571, 1103, 2231, 2244
AcoI YGGCCR 1 cut(s) 29
AcsI RAATTY 6 cut(s) 752, 877, 889, 1033, 1039, 1087
AcuI CTGAAG 7 cut(s) 174, 185, 333, 342, 438, 840, 1244
AfaI GTAC 4 cut(s) 1019, 1486, 1875, 2529
AfiI CCNNNNNNNGG 5 cut(s) 1167, 1232, 1436, 1469, 2362
AflIII ACRYGT 4 cut(s) 613, 1785, 1924, 2029
AjiI CACGTC 1 cut(s) 2180
AjnI CCWGG 6 cut(s) 384, 655, 1171, 1741, 2056, 2140
AleI CACNNNNGTG 1 cut(s) 2190
AloI GAACNNNNNNTCC 4 cut(s) 1115, 1116, 1147, 1148
AluBI AGCT 8 cut(s) 167, 205, 964, 975, 1379, 1445, 1530, 1824
AluI AGCT 8 cut(s) 167, 205, 964, 975, 1379, 1445, 1530, 1824
Alw21I GWGCWC 4 cut(s) 1240, 1532, 1785, 2022
Alw26I GTCTC 2 cut(s) 867, 958
Alw44I GTGCAC 1 cut(s) 2018
AlwI GGATC 5 cut(s) 558, 571, 1103, 2231, 2244
Ama87I CYCGRG 1 cut(s) 2397
AoxI GGCC 9 cut(s) 29, 113, 795, 1169, 1274, 1460, 1812, 2047, 2059
ApaLI GTGCAC 1 cut(s) 2018
ApeKI GCWGC 5 cut(s) 326, 869, 961, 1883, 2333
ApoI RAATTY 6 cut(s) 752, 877, 889, 1033, 1039, 1087
ArsI GACNNNNNNTTYG 4 cut(s) 2337, 2369, 2552, 2584
AseI ATTAAT 2 cut(s) 1254, 1344
Asp700I GAANNNNTTC 2 cut(s) 198, 1926
AspLEI GCGC 1 cut(s) 271
AspS9I GGNCC 5 cut(s) 46, 113, 1739, 2008, 2138
AsuC2I CCSGG 3 cut(s) 237, 2398, 2399
AsuHPI GGTGA 7 cut(s) 23, 728, 1096, 1212, 1419, 1451, 2461
AsuII TTCGAA 1 cut(s) 515
AvaI CYCGRG 1 cut(s) 2397
AvaII GGWCC 4 cut(s) 46, 1739, 2008, 2138
BaeGI GKGCMC 3 cut(s) 1996, 2022, 2179
BamHI GGATCC 2 cut(s) 563, 2236
BanI GGYRCC 1 cut(s) 1595
BanII GRGCYC 2 cut(s) 1070, 1532
BarI GAAGNNNNNNTAC 4 cut(s) 1004, 1036, 2536, 2568
BbsI GAAGAC 2 cut(s) 2168, 2174
Bbv12I GWGCWC 4 cut(s) 1240, 1532, 1785, 2022
BbvCI CCTCAGC 1 cut(s) 1887
BbvI GCAGC 5 cut(s) 338, 856, 973, 1870, 2345
BccI CCATC 7 cut(s) 293, 1226, 1774, 1796, 1967, 2031, 2139
BceAI ACGGC 2 cut(s) 1184, 2571
BcgI CGANNNNNNTGC 2 cut(s) 2547, 2581
BciT130I CCWGG 6 cut(s) 386, 657, 1173, 1743, 2058, 2142
BcnI CCSGG 3 cut(s) 237, 2398, 2399
BcoDI GTCTC 2 cut(s) 867, 958
BfaI CTAG 5 cut(s) 60, 446, 498, 1380, 1968
BfmI CTRYAG 4 cut(s) 36, 477, 690, 1193
BfoI RGCGCY 1 cut(s) 272
BfuAI ACCTGC 2 cut(s) 1192, 1200
BglII AGATCT 1 cut(s) 2479
BisI GCNGC 8 cut(s) 29, 32, 327, 870, 962, 1884, 2334, 2443
BlsI GCNGC 8 cut(s) 30, 33, 328, 871, 963, 1885, 2335, 2444
BmcAI AGTACT 3 cut(s) 1019, 1875, 2529
Bme1390I CCNGG 9 cut(s) 237, 386, 657, 1173, 1743, 2058, 2142, 2398, 2399
Bme18I GGWCC 4 cut(s) 46, 1739, 2008, 2138
BmeT110I CYCGRG 1 cut(s) 2397
BmgBI CACGTC 1 cut(s) 2180
BmgT120I GGNCC 5 cut(s) 46, 113, 1739, 2008, 2138
BmiI GGNNCC 5 cut(s) 114, 565, 1597, 2010, 2238
BmrFI CCNGG 9 cut(s) 237, 386, 657, 1173, 1743, 2058, 2142, 2398, 2399
BmrI ACTGGG 1 cut(s) 25
BmsI GCATC 5 cut(s) 124, 610, 1405, 1718, 2302
BmuI ACTGGG 1 cut(s) 25
BpiI GAAGAC 2 cut(s) 2168, 2174
BplI GAGNNNNNCTC 2 cut(s) 1464, 1496
BpmI CTGGAG 3 cut(s) 407, 1761, 2488
Bpu10I CCTNAGC 1 cut(s) 1887
Bpu14I TTCGAA 1 cut(s) 515
BpuEI CTTGAG 2 cut(s) 392, 1890
BpuMI CCSGG 3 cut(s) 237, 2398, 2399
Bsa29I ATCGAT 1 cut(s) 669
BsaBI GATNNNNATC 5 cut(s) 402, 568, 930, 1107, 1708
BsaJI CCNNGG 5 cut(s) 1062, 1226, 1836, 2396, 2397
BsaXI ACNNNNNCTCC 6 cut(s) 156, 186, 217, 247, 1207, 1237
Bsc4I CCNNNNNNNGG 5 cut(s) 1167, 1232, 1436, 1469, 2362
Bse1I ACTGG 5 cut(s) 20, 232, 1651, 1730, 1815
Bse3DI GCAATG 3 cut(s) 157, 956, 1939
Bse8I GATNNNNATC 5 cut(s) 402, 568, 930, 1107, 1708
BseBI CCWGG 6 cut(s) 386, 657, 1173, 1743, 2058, 2142
BseCI ATCGAT 1 cut(s) 669
BseDI CCNNGG 5 cut(s) 1062, 1226, 1836, 2396, 2397
BseGI GGATG 6 cut(s) 748, 931, 1237, 1709, 2150, 2293
BseJI GATNNNNATC 5 cut(s) 402, 568, 930, 1107, 1708
BseLI CCNNNNNNNGG 5 cut(s) 1167, 1232, 1436, 1469, 2362
BseMI GCAATG 3 cut(s) 157, 956, 1939
BseMII CTCAG 3 cut(s) 78, 816, 1878
BseNI ACTGG 5 cut(s) 20, 232, 1651, 1730, 1815
BseRI GAGGAG 2 cut(s) 1230, 2142
BseSI GKGCMC 3 cut(s) 1996, 2022, 2179
BseX3I CGGCCG 1 cut(s) 29
BseXI GCAGC 5 cut(s) 338, 856, 973, 1870, 2345
BsgI GTGCAG 2 cut(s) 2274, 2373
Bsh1236I CGCG 1 cut(s) 2442
Bsh1285I CGRYCG 1 cut(s) 32
BshFI GGCC 9 cut(s) 31, 115, 797, 1171, 1276, 1462, 1814, 2049, 2061
BshNI GGYRCC 1 cut(s) 1595
BshVI ATCGAT 1 cut(s) 669
BsiEI CGRYCG 1 cut(s) 32
BsiHKAI GWGCWC 4 cut(s) 1240, 1532, 1785, 2022
BsiHKCI CYCGRG 1 cut(s) 2397
BsiSI CCGG 3 cut(s) 236, 2398, 2571
BslI CCNNNNNNNGG 5 cut(s) 1167, 1232, 1436, 1469, 2362
BsmAI GTCTC 2 cut(s) 867, 958
BsmI GAATGC 2 cut(s) 262, 1307
BsnI GGCC 9 cut(s) 31, 115, 797, 1171, 1276, 1462, 1814, 2049, 2061
BsoBI CYCGRG 1 cut(s) 2397
Bsp119I TTCGAA 1 cut(s) 515
Bsp1286I GDGCHC 7 cut(s) 1070, 1240, 1532, 1785, 1996, 2022, 2179
Bsp143I GATC 5 cut(s) 563, 1108, 1432, 2236, 2479
Bsp19I CCATGG 2 cut(s) 1226, 1836
BspACI CCGC 6 cut(s) 28, 32, 329, 1359, 1869, 2442
BspANI GGCC 9 cut(s) 31, 115, 797, 1171, 1276, 1462, 1814, 2049, 2061
BspCNI CTCAG 3 cut(s) 79, 817, 1879
BspDI ATCGAT 1 cut(s) 669
BspFNI CGCG 1 cut(s) 2442
BspLI GGNNCC 5 cut(s) 114, 565, 1597, 2010, 2238
BspMAI CTGCAG 3 cut(s) 40, 694, 1197
BspMI ACCTGC 2 cut(s) 1192, 1200
BspPI GGATC 5 cut(s) 558, 571, 1103, 2231, 2244
BspT104I TTCGAA 1 cut(s) 515
BspT107I GGYRCC 1 cut(s) 1595
BsrDI GCAATG 3 cut(s) 157, 956, 1939
BsrI ACTGG 5 cut(s) 20, 232, 1651, 1730, 1815
BssECI CCNNGG 5 cut(s) 1062, 1226, 1836, 2396, 2397
BssMI GATC 5 cut(s) 563, 1108, 1432, 2236, 2479
BssNAI GTATAC 1 cut(s) 361
BssT1I CCWWGG 3 cut(s) 1062, 1226, 1836
Bst1107I GTATAC 1 cut(s) 361
Bst2UI CCWGG 6 cut(s) 386, 657, 1173, 1743, 2058, 2142
Bst4CI ACNGT 9 cut(s) 11, 580, 2192, 2276, 2365, 2455, 2521, 2598, 2617
Bst6I CTCTTC 2 cut(s) 204, 759
BstAPI GCANNNNNTGC 1 cut(s) 2339
BstBI TTCGAA 1 cut(s) 515
BstC8I GCNNGC 7 cut(s) 36, 117, 553, 1528, 1600, 1812, 2440
BstDEI CTNAG 4 cut(s) 87, 825, 840, 1887
BstDSI CCRYGG 2 cut(s) 1226, 1836
BstF5I GGATG 6 cut(s) 748, 931, 1237, 1709, 2150, 2293
BstFNI CGCG 1 cut(s) 2442
BstH2I RGCGCY 1 cut(s) 272
BstHHI GCGC 1 cut(s) 271
BstKTI GATC 5 cut(s) 566, 1111, 1435, 2239, 2482
BstMAI GTCTC 2 cut(s) 867, 958
BstMBI GATC 5 cut(s) 563, 1108, 1432, 2236, 2479
BstMCI CGRYCG 1 cut(s) 32
BstMWI GCNNNNNNNGC 7 cut(s) 266, 323, 776, 981, 1451, 2174, 2339
BstNI CCWGG 6 cut(s) 386, 657, 1173, 1743, 2058, 2142
BstNSI RCATGY 6 cut(s) 617, 737, 1789, 1928, 2033, 2201
BstSCI CCNGG 9 cut(s) 235, 384, 655, 1171, 1741, 2056, 2140, 2396, 2397
BstSFI CTRYAG 4 cut(s) 36, 477, 690, 1193
BstSLI GKGCMC 3 cut(s) 1996, 2022, 2179
BstUI CGCG 1 cut(s) 2442
BstV1I GCAGC 5 cut(s) 338, 856, 973, 1870, 2345
BstV2I GAAGAC 2 cut(s) 2168, 2174
BstX2I RGATCY 4 cut(s) 563, 1108, 2236, 2479
BstXI CCANNNNNNTGG 1 cut(s) 1614
BstYI RGATCY 4 cut(s) 563, 1108, 2236, 2479
BstZ17I GTATAC 1 cut(s) 361
BstZI CGGCCG 1 cut(s) 29
Bsu15I ATCGAT 1 cut(s) 669
BsuRI GGCC 9 cut(s) 31, 115, 797, 1171, 1276, 1462, 1814, 2049, 2061
BsuTUI ATCGAT 1 cut(s) 669
BtgI CCRYGG 2 cut(s) 1226, 1836
BtrI CACGTC 1 cut(s) 2180
BtsCI GGATG 6 cut(s) 748, 931, 1237, 1709, 2150, 2293
BtsI GCAGTG 2 cut(s) 2109, 2286
BtsIMutI CAGTG 5 cut(s) 1822, 2109, 2197, 2286, 2517
BveI ACCTGC 2 cut(s) 1192, 1200
Cac8I GCNNGC 7 cut(s) 36, 117, 553, 1528, 1600, 1812, 2440
CciNI GCGGCCGC 1 cut(s) 29
CfoI GCGC 1 cut(s) 271
Cfr13I GGNCC 5 cut(s) 46, 113, 1739, 2008, 2138
Cfr9I CCCGGG 1 cut(s) 2397
ClaI ATCGAT 1 cut(s) 669
Csp6I GTAC 4 cut(s) 1018, 1485, 1874, 2528
CspCI CAANNNNNGTGG 4 cut(s) 322, 357, 1945, 1980
CviQI GTAC 4 cut(s) 1018, 1485, 1874, 2528
DdeI CTNAG 4 cut(s) 87, 825, 840, 1887
DpnI GATC 5 cut(s) 565, 1110, 1434, 2238, 2481
DpnII GATC 5 cut(s) 563, 1108, 1432, 2236, 2479
DraI TTTAAA 2 cut(s) 811, 1096
DrdI GACNNNNNNGTC 1 cut(s) 859
DseDI GACNNNNNNGTC 1 cut(s) 859
EaeI YGGCCR 1 cut(s) 29
EagI CGGCCG 1 cut(s) 29
Eam1104I CTCTTC 2 cut(s) 204, 759
EarI CTCTTC 2 cut(s) 204, 759
Ecl136II GAGCTC 1 cut(s) 1530
EclXI CGGCCG 1 cut(s) 29
Eco130I CCWWGG 3 cut(s) 1062, 1226, 1836
Eco24I GRGCYC 2 cut(s) 1070, 1532
Eco32I GATATC 2 cut(s) 546, 1148
Eco47I GGWCC 4 cut(s) 46, 1739, 2008, 2138
Eco52I CGGCCG 1 cut(s) 29
Eco53kI GAGCTC 1 cut(s) 1530
Eco57I CTGAAG 7 cut(s) 174, 185, 333, 342, 438, 840, 1244
Eco88I CYCGRG 1 cut(s) 2397
EcoICRI GAGCTC 1 cut(s) 1530
EcoO109I RGGNCCY 4 cut(s) 46, 113, 1739, 2008
EcoRI GAATTC 2 cut(s) 877, 1087
EcoRII CCWGG 6 cut(s) 384, 655, 1171, 1741, 2056, 2140
EcoRV GATATC 2 cut(s) 546, 1148
EcoT14I CCWWGG 3 cut(s) 1062, 1226, 1836
EcoT22I ATGCAT 1 cut(s) 2203
EcoT38I GRGCYC 2 cut(s) 1070, 1532
ErhI CCWWGG 3 cut(s) 1062, 1226, 1836
FalI AAGNNNNNCTT 4 cut(s) 838, 870, 2466, 2498
FblI GTMKAC 2 cut(s) 360, 2150
Fnu4HI GCNGC 8 cut(s) 29, 32, 327, 870, 962, 1884, 2334, 2443
FokI GGATG 6 cut(s) 755, 938, 1244, 1716, 2157, 2280
FriOI GRGCYC 2 cut(s) 1070, 1532
Fsp4HI GCNGC 8 cut(s) 29, 32, 327, 870, 962, 1884, 2334, 2443
FspBI CTAG 5 cut(s) 60, 446, 498, 1380, 1968
GlaI GCGC 1 cut(s) 270
GluI GCNGC 8 cut(s) 29, 32, 327, 870, 962, 1884, 2334, 2443
GsuI CTGGAG 3 cut(s) 407, 1761, 2488
HaeII RGCGCY 1 cut(s) 272
HaeIII GGCC 9 cut(s) 31, 115, 797, 1171, 1276, 1462, 1814, 2049, 2061
HapII CCGG 3 cut(s) 236, 2398, 2571
HhaI GCGC 1 cut(s) 271
Hin6I GCGC 1 cut(s) 269
HinP1I GCGC 1 cut(s) 269
HincII GTYRAC 2 cut(s) 1369, 2458
HindII GTYRAC 2 cut(s) 1369, 2458
HinfI GANTC 9 cut(s) 55, 398, 517, 666, 679, 836, 860, 937, 1628
HpaII CCGG 3 cut(s) 236, 2398, 2571
HphI GGTGA 7 cut(s) 23, 728, 1096, 1212, 1419, 1451, 2461
Hpy188I TCNGA 9 cut(s) 88, 193, 835, 852, 859, 904, 936, 1263, 2506
HpyCH4III ACNGT 9 cut(s) 11, 580, 2192, 2276, 2365, 2455, 2521, 2598, 2617
HpyCH4IV ACGT 2 cut(s) 1410, 2179
HpyF10VI GCNNNNNNNGC 7 cut(s) 266, 323, 776, 981, 1451, 2174, 2339
HpyF3I CTNAG 4 cut(s) 87, 825, 840, 1887
HpySE526I ACGT 2 cut(s) 1410, 2179
HspAI GCGC 1 cut(s) 269
Kzo9I GATC 5 cut(s) 563, 1108, 1432, 2236, 2479
LmnI GCTCC 7 cut(s) 164, 265, 502, 1243, 1472, 1535, 1801
Lsp1109I GCAGC 5 cut(s) 338, 856, 973, 1870, 2345
LweI GCATC 5 cut(s) 124, 610, 1405, 1718, 2302
MaeI CTAG 5 cut(s) 60, 446, 498, 1380, 1968
MaeII ACGT 2 cut(s) 1410, 2179
MalI GATC 5 cut(s) 565, 1110, 1434, 2238, 2481
MboI GATC 5 cut(s) 563, 1108, 1432, 2236, 2479
MfeI CAATTG 2 cut(s) 144, 1662
MflI RGATCY 4 cut(s) 563, 1108, 2236, 2479
MhlI GDGCHC 7 cut(s) 1070, 1240, 1532, 1785, 1996, 2022, 2179
MlyI GAGTC 3 cut(s) 49, 869, 1637
MmeI TCCRAC 5 cut(s) 875, 1599, 1631, 1677, 2484
Mph1103I ATGCAT 1 cut(s) 2203
MroXI GAANNNNTTC 2 cut(s) 198, 1926
MslI CAYNNNNRTG 5 cut(s) 618, 924, 1225, 2028, 2190
MspA1I CMGCKG 1 cut(s) 329
MspI CCGG 3 cut(s) 236, 2398, 2571
MspR9I CCNGG 9 cut(s) 237, 386, 657, 1173, 1743, 2058, 2142, 2398, 2399
MunI CAATTG 2 cut(s) 144, 1662
Mva1269I GAATGC 2 cut(s) 262, 1307
MvaI CCWGG 6 cut(s) 386, 657, 1173, 1743, 2058, 2142
MvnI CGCG 1 cut(s) 2442
MwoI GCNNNNNNNGC 7 cut(s) 266, 323, 776, 981, 1451, 2174, 2339
NciI CCSGG 3 cut(s) 237, 2398, 2399
NcoI CCATGG 2 cut(s) 1226, 1836
NdeII GATC 5 cut(s) 563, 1108, 1432, 2236, 2479
NlaIV GGNNCC 5 cut(s) 114, 565, 1597, 2010, 2238
NmuCI GTSAC 6 cut(s) 11, 535, 716, 1052, 1402, 2449
NotI GCGGCCGC 1 cut(s) 29
NsiI ATGCAT 1 cut(s) 2203
NspI RCATGY 6 cut(s) 617, 737, 1789, 1928, 2033, 2201
NspV TTCGAA 1 cut(s) 515
OliI CACNNNNGTG 1 cut(s) 2190
PciI ACATGT 4 cut(s) 613, 1785, 1924, 2029
PctI GAATGC 2 cut(s) 262, 1307
PdmI GAANNNNTTC 2 cut(s) 198, 1926
PfeI GAWTC 6 cut(s) 398, 517, 666, 679, 836, 937
PflMI CCANNNNNTGG 1 cut(s) 1232
PfoI TCCNGGA 1 cut(s) 2140
PkrI GCNGC 8 cut(s) 30, 33, 328, 871, 963, 1885, 2335, 2444
PleI GAGTC 3 cut(s) 49, 868, 1636
PpsI GAGTC 3 cut(s) 49, 868, 1636
PpuMI RGGWCCY 3 cut(s) 46, 1739, 2008
PscI ACATGT 4 cut(s) 613, 1785, 1924, 2029
PshBI ATTAAT 2 cut(s) 1254, 1344
PsiI TTATAA 1 cut(s) 2121
Psp124BI GAGCTC 1 cut(s) 1532
Psp5II RGGWCCY 3 cut(s) 46, 1739, 2008
Psp6I CCWGG 6 cut(s) 384, 655, 1171, 1741, 2056, 2140
PspGI CCWGG 6 cut(s) 384, 655, 1171, 1741, 2056, 2140
PspN4I GGNNCC 5 cut(s) 114, 565, 1597, 2010, 2238
PspPI GGNCC 5 cut(s) 46, 113, 1739, 2008, 2138
PspPPI RGGWCCY 3 cut(s) 46, 1739, 2008
PstI CTGCAG 3 cut(s) 40, 694, 1197
PsuI RGATCY 4 cut(s) 563, 1108, 2236, 2479
RsaI GTAC 4 cut(s) 1019, 1486, 1875, 2529
RsaNI GTAC 4 cut(s) 1018, 1485, 1874, 2528
RseI CAYNNNNRTG 5 cut(s) 618, 924, 1225, 2028, 2190
SacI GAGCTC 1 cut(s) 1532
SatI GCNGC 8 cut(s) 29, 32, 327, 870, 962, 1884, 2334, 2443
Sau3AI GATC 5 cut(s) 563, 1108, 1432, 2236, 2479
Sau96I GGNCC 5 cut(s) 46, 113, 1739, 2008, 2138
SbfI CCTGCAGG 1 cut(s) 1197
ScaI AGTACT 3 cut(s) 1019, 1875, 2529
SchI GAGTC 3 cut(s) 49, 869, 1637
ScrFI CCNGG 9 cut(s) 237, 386, 657, 1173, 1743, 2058, 2142, 2398, 2399
SdaI CCTGCAGG 1 cut(s) 1197
SduI GDGCHC 7 cut(s) 1070, 1240, 1532, 1785, 1996, 2022, 2179
SfaNI GCATC 5 cut(s) 124, 610, 1405, 1718, 2302
SfcI CTRYAG 4 cut(s) 36, 477, 690, 1193
SfuI TTCGAA 1 cut(s) 515
SinI GGWCC 4 cut(s) 46, 1739, 2008, 2138
SmaI CCCGGG 1 cut(s) 2399
SmiMI CAYNNNNRTG 5 cut(s) 618, 924, 1225, 2028, 2190
SmlI CTYRAG 2 cut(s) 407, 1905
SmoI CTYRAG 2 cut(s) 407, 1905
Sse8387I CCTGCAGG 1 cut(s) 1197
SsiI CCGC 6 cut(s) 28, 32, 329, 1359, 1869, 2442
SspI AATATT 1 cut(s) 473
SspMI CTAG 5 cut(s) 60, 446, 498, 1380, 1968
SstI GAGCTC 1 cut(s) 1532
StyD4I CCNGG 9 cut(s) 235, 384, 655, 1171, 1741, 2056, 2140, 2396, 2397
StyI CCWWGG 3 cut(s) 1062, 1226, 1836
TaaI ACNGT 9 cut(s) 11, 580, 2192, 2276, 2365, 2455, 2521, 2598, 2617
TaiI ACGT 2 cut(s) 1413, 2182
TaqI TCGA 5 cut(s) 515, 669, 1144, 1435, 2026
TatI WGTACW 4 cut(s) 1017, 1484, 1873, 2527
TauI GCSGC 3 cut(s) 31, 34, 2445
TfiI GAWTC 6 cut(s) 398, 517, 666, 679, 836, 937
TscAI CASTG 5 cut(s) 1822, 2109, 2197, 2293, 2524
TseFI GTSAC 6 cut(s) 11, 535, 716, 1052, 1402, 2449
TseI GCWGC 5 cut(s) 326, 869, 961, 1883, 2333
Tsp45I GTSAC 6 cut(s) 11, 535, 716, 1052, 1402, 2449
TspDTI ATGAA 9 cut(s) 764, 795, 935, 1005, 1025, 1119, 1280, 1389, 1627
TspMI CCCGGG 1 cut(s) 2397
TspRI CASTG 5 cut(s) 1822, 2109, 2197, 2293, 2524
Van91I CCANNNNNTGG 1 cut(s) 1232
VneI GTGCAC 1 cut(s) 2018
VpaK11BI GGWCC 4 cut(s) 46, 1739, 2008, 2138
VspI ATTAAT 2 cut(s) 1254, 1344
XapI RAATTY 6 cut(s) 752, 877, 889, 1033, 1039, 1087
XceI RCATGY 6 cut(s) 617, 737, 1789, 1928, 2033, 2201
XmaI CCCGGG 1 cut(s) 2397
XmiI GTMKAC 2 cut(s) 360, 2150
XmnI GAANNNNTTC 2 cut(s) 198, 1926
XspI CTAG 5 cut(s) 60, 446, 498, 1380, 1968
ZrmI AGTACT 3 cut(s) 1019, 1875, 2529
Zsp2I ATGCAT 1 cut(s) 2203
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.