FvH4_2g05241

zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0005730
Physical Location & Seq
Reverse (-)
4330075 .. 4335234
5160 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g05241.t4

Sequence Viewer

Length: 1458 bp
ATGGGCAAGAGGGAGAAGCAAGCAAGCAAGTTTCATTGGGACGAATTAAGTGAGGGAGAAGGAGAATCAGCTAATAGGAAAGCCTTGAAATCTGTAGTACAGATCAGTAGCGATGACGAGGCCAATGAAGATCTCAGCCTCAAAATAGTTGAAAAAGCTTTGCTGATGCGTGCTGCCAAACTGGCCTCAGAGAACGACTCAGCTGCTTCCGATCTAGGTGGTGTGACTTCTTCGTCTTCTCCACAGGTTGCTGAAGTTGAGGCCGCAGATCTGGAGACTAAGAAAGTAGTTAAGAAGGACAGGGTGAGGAAGAGTAAAAAGAGGAAAATTGAAGACCCAGCTGTGAATCTTGCTAAAGAAGAAGTGAAGGTAGACGAAGGTGGAGCTGTAGATGAGACAATTGAACTGAACAATGTTCAGATCACTGACAATATTGTGCTGCGGAAACTTCTTCGGGGACCAAGGTATTTTGATCCTCCAGATAAGAGCTGGGGCGCATGCTTTAATTGCGGTGAAGAAGGGCATGCAGCAGTGAACTGTACCGTAGCTAAGCGGAAGAAGCCATGCTTTGTTTGTGGTAGTTTGGAGCACAATGCCAAGCAATGTGAAAAGGGACATGATTGCTTTATCTGCAAAAAGGGTGGACATCATGCTAAAAATTGTCCAGAGAAGTACAATGTTGGCTCATTGACTTCCAAAATATGTTTAAAATGTGGAGATTCTGGGCATGAGATGTATTCATGCAAGAATGATTACCCATCTGATGACCTCAAGGAAATTCAATGTTATGTCTGCAAGAAATTTGGTCATTTATGTTGTGTCAAGTGTGGTGATAACAGTGTAAAGGAAGTATCTTGTTACAGATGTGGTCAACTGGGACACACTGGTATGGCGTGCATAGGCTTCCGTAGTGGGGAAACCACTGTGTCTGAAACCCCTCGTTTATGCTACAAATGTGGTGAAGGTGGTCATATTGCTCGGGAATGCAGAACCTCCAATAATGCTCGTAAGAGGTATGATGAGTTCTCTAACGTATCTACCCCGACTTTGAGACCTCATAAAGAAAAGAAAGACTATATGGGATTCTATTCTGCTCCTAATGATCTGAATAAGAATAAAAGGAAACATACCTACTATGATGATAGAGGCTTTACAACTCCACAAAAAGCAATACATAGAGGTGGGTGGATAGTGGATGATCCAGATGACTTCTCCCCTAGAAAAGGCAAGAACGGCTGGAAGTCCCCTGCAACACCATCTAGTAGGAGCCATAGGATTCCAAGTTCGACTGCAGGCAGTCATGCTTCAAGTTCTAGATCCTCTAAAAAGTTATGGAAGGTTTCTGGAGGAACTCCTATCTCACAAGGATCATCTAAGTCTGTTCATCACAGATATTCTGCACAGAGGTTTAACAACTCTGGTACTGATGGTATGAGAAGAAATAACGAGTGGTGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-CCHC

Protein Analysis

486

Amino Acids

53.76

Weight (kDa)

9.02

Isoelectric Point (pI)

42.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-CCHC PF00098 315 - 330 2.5e-06 Zinc knuckle
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 232
AccI GTMKAC 1 cut(s) 372
AciI CCGC 4 cut(s) 264, 442, 510, 553
AclWI GGATC 4 cut(s) 467, 1193, 1311, 1375
AcsI RAATTY 2 cut(s) 777, 800
AcuI CTGAAG 1 cut(s) 273
AfaI GTAC 4 cut(s) 99, 541, 674, 1423
AfiI CCNNNNNNNGG 2 cut(s) 913, 1223
AgsI TTSAA 6 cut(s) 88, 152, 332, 404, 782, 1308
AloI GAACNNNNNNTCC 2 cut(s) 1267, 1299
AluBI AGCT 7 cut(s) 71, 158, 203, 341, 386, 489, 548
AluI AGCT 7 cut(s) 71, 158, 203, 341, 386, 489, 548
Alw21I GWGCWC 1 cut(s) 591
Alw26I GTCTC 3 cut(s) 269, 389, 1045
AlwI GGATC 4 cut(s) 467, 1193, 1311, 1375
Ama87I CYCGRG 1 cut(s) 978
AoxI GGCC 3 cut(s) 120, 183, 261
ApeKI GCWGC 4 cut(s) 173, 203, 439, 527
ApoI RAATTY 2 cut(s) 777, 800
AspLEI GCGC 1 cut(s) 497
AspS9I GGNCC 1 cut(s) 458
AsuHPI GGTGA 4 cut(s) 316, 524, 842, 971
AvaI CYCGRG 1 cut(s) 978
AvaII GGWCC 1 cut(s) 458
BbsI GAAGAC 2 cut(s) 228, 339
Bbv12I GWGCWC 1 cut(s) 591
BbvI GCAGC 4 cut(s) 160, 190, 426, 539
BccI CCATC 3 cut(s) 766, 1264, 1421
BceAI ACGGC 1 cut(s) 1249
BcgI CGANNNNNNTGC 2 cut(s) 185, 219
BcoDI GTCTC 3 cut(s) 269, 389, 1045
BfaI CTAG 4 cut(s) 215, 1218, 1260, 1314
BfmI CTRYAG 3 cut(s) 93, 387, 1290
BglI GCCNNNNNGGC 1 cut(s) 182
BglII AGATCT 2 cut(s) 130, 268
BisI GCNGC 5 cut(s) 174, 204, 264, 440, 528
BlpI GCTNAGC 1 cut(s) 549
BlsI GCNGC 5 cut(s) 175, 205, 265, 441, 529
Bme18I GGWCC 1 cut(s) 458
BmeT110I CYCGRG 1 cut(s) 978
BmgT120I GGNCC 1 cut(s) 458
BmiI GGNNCC 2 cut(s) 459, 1268
BmrI ACTGGG 1 cut(s) 884
BmsI GCATC 1 cut(s) 156
BmuI ACTGGG 1 cut(s) 884
BpiI GAAGAC 2 cut(s) 228, 339
BplI GAGNNNNNCTC 2 cut(s) 182, 214
BpmI CTGGAG 3 cut(s) 293, 462, 1365
Bpu1102I GCTNAGC 1 cut(s) 549
BpuEI CTTGAG 1 cut(s) 755
BsaI GGTCTC 1 cut(s) 1045
BsaJI CCNNGG 1 cut(s) 461
Bsc4I CCNNNNNNNGG 2 cut(s) 913, 1223
Bse1I ACTGG 3 cut(s) 186, 879, 889
Bse3DI GCAATG 1 cut(s) 608
BseDI CCNNGG 1 cut(s) 461
BseGI GGATG 1 cut(s) 1201
BseLI CCNNNNNNNGG 2 cut(s) 913, 1223
BseMI GCAATG 1 cut(s) 608
BseMII CTCAG 3 cut(s) 148, 201, 213
BseNI ACTGG 3 cut(s) 186, 879, 889
BseXI GCAGC 4 cut(s) 160, 190, 426, 539
BseYI CCCAGC 2 cut(s) 337, 489
BsgI GTGCAG 1 cut(s) 1383
BshFI GGCC 3 cut(s) 122, 185, 263
BsiHKAI GWGCWC 1 cut(s) 591
BsiHKCI CYCGRG 1 cut(s) 978
BslFI GGGAC 5 cut(s) 53, 471, 627, 891, 1228
BslI CCNNNNNNNGG 2 cut(s) 913, 1223
BsmAI GTCTC 3 cut(s) 269, 389, 1045
BsmFI GGGAC 5 cut(s) 53, 471, 627, 891, 1228
BsmI GAATGC 1 cut(s) 989
BsnI GGCC 3 cut(s) 122, 185, 263
Bso31I GGTCTC 1 cut(s) 1045
BsoBI CYCGRG 1 cut(s) 978
Bsp1286I GDGCHC 1 cut(s) 591
Bsp1720I GCTNAGC 1 cut(s) 549
BspACI CCGC 4 cut(s) 264, 442, 510, 553
BspANI GGCC 3 cut(s) 122, 185, 263
BspCNI CTCAG 3 cut(s) 147, 200, 212
BspLI GGNNCC 2 cut(s) 459, 1268
BspMAI CTGCAG 1 cut(s) 1294
BspPI GGATC 4 cut(s) 467, 1193, 1311, 1375
BspTNI GGTCTC 1 cut(s) 1045
BsrDI GCAATG 1 cut(s) 608
BsrI ACTGG 3 cut(s) 186, 879, 889
BssECI CCNNGG 1 cut(s) 461
BssT1I CCWWGG 1 cut(s) 461
Bst4CI ACNGT 4 cut(s) 539, 544, 839, 925
Bst6I CTCTTC 1 cut(s) 305
BstC8I GCNNGC 7 cut(s) 21, 25, 171, 499, 525, 895, 1294
BstDEI CTNAG 6 cut(s) 134, 187, 199, 279, 549, 1374
BstENI CCTNNNNNAGG 1 cut(s) 1221
BstF5I GGATG 1 cut(s) 1201
BstHHI GCGC 1 cut(s) 497
BstMAI GTCTC 3 cut(s) 269, 389, 1045
BstMWI GCNNNNNNNGC 5 cut(s) 182, 507, 559, 630, 1233
BstNSI RCATGY 2 cut(s) 501, 527
BstSFI CTRYAG 3 cut(s) 93, 387, 1290
BstV1I GCAGC 4 cut(s) 160, 190, 426, 539
BstV2I GAAGAC 2 cut(s) 228, 339
BstX2I RGATCY 3 cut(s) 130, 268, 1316
BstYI RGATCY 3 cut(s) 130, 268, 1316
BsuRI GGCC 3 cut(s) 122, 185, 263
BtgZI GCGATG 1 cut(s) 126
BtsCI GGATG 1 cut(s) 1201
BtsI GCAGTG 1 cut(s) 537
BtsIMutI CAGTG 5 cut(s) 423, 537, 844, 882, 921
Cac8I GCNNGC 7 cut(s) 21, 25, 171, 499, 525, 895, 1294
CfoI GCGC 1 cut(s) 497
Cfr13I GGNCC 1 cut(s) 458
Csp6I GTAC 4 cut(s) 98, 540, 673, 1422
CspCI CAANNNNNGTGG 2 cut(s) 622, 657
CviAII CATG 8 cut(s) 498, 524, 564, 617, 650, 728, 741, 1301
CviQI GTAC 4 cut(s) 98, 540, 673, 1422
DdeI CTNAG 6 cut(s) 134, 187, 199, 279, 549, 1374
DraI TTTAAA 1 cut(s) 708
DrdI GACNNNNNNGTC 1 cut(s) 232
DseDI GACNNNNNNGTC 1 cut(s) 232
Eam1104I CTCTTC 1 cut(s) 305
EarI CTCTTC 1 cut(s) 305
Eco130I CCWWGG 1 cut(s) 461
Eco31I GGTCTC 1 cut(s) 1045
Eco47I GGWCC 1 cut(s) 458
Eco57I CTGAAG 1 cut(s) 273
Eco88I CYCGRG 1 cut(s) 978
EcoNI CCTNNNNNAGG 1 cut(s) 1221
EcoT14I CCWWGG 1 cut(s) 461
ErhI CCWWGG 1 cut(s) 461
FaeI CATG 8 cut(s) 501, 527, 567, 620, 653, 731, 744, 1304
FalI AAGNNNNNCTT 2 cut(s) 551, 583
FaqI GGGAC 5 cut(s) 53, 471, 627, 891, 1228
FatI CATG 8 cut(s) 497, 523, 563, 616, 649, 727, 740, 1300
FblI GTMKAC 1 cut(s) 372
Fnu4HI GCNGC 5 cut(s) 174, 204, 264, 440, 528
FokI GGATG 1 cut(s) 1208
Fsp4HI GCNGC 5 cut(s) 174, 204, 264, 440, 528
FspBI CTAG 4 cut(s) 215, 1218, 1260, 1314
GlaI GCGC 1 cut(s) 496
GluI GCNGC 5 cut(s) 174, 204, 264, 440, 528
GsaI CCCAGC 2 cut(s) 341, 493
GsuI CTGGAG 3 cut(s) 293, 462, 1365
HaeIII GGCC 3 cut(s) 122, 185, 263
HhaI GCGC 1 cut(s) 497
Hin1II CATG 8 cut(s) 501, 527, 567, 620, 653, 731, 744, 1304
Hin6I GCGC 1 cut(s) 495
HinP1I GCGC 1 cut(s) 495
HincII GTYRAC 1 cut(s) 872
HindII GTYRAC 1 cut(s) 872
HindIII AAGCTT 1 cut(s) 156
HinfI GANTC 6 cut(s) 65, 197, 346, 719, 1083, 1276
HphI GGTGA 4 cut(s) 316, 524, 842, 971
Hpy166II GTNNAC 4 cut(s) 373, 535, 644, 872
Hpy188I TCNGA 6 cut(s) 190, 211, 420, 763, 931, 1107
Hpy188III TCNNGA 7 cut(s) 272, 479, 665, 980, 1202, 1314, 1344
Hpy8I GTNNAC 4 cut(s) 373, 535, 644, 872
HpyAV CCTTC 7 cut(s) 53, 289, 361, 371, 512, 956, 1330
HpyCH4III ACNGT 4 cut(s) 539, 544, 839, 925
HpyCH4IV ACGT 1 cut(s) 1032
HpyCH4V TGCA 9 cut(s) 527, 633, 744, 795, 897, 987, 1250, 1292, 1400
HpyF10VI GCNNNNNNNGC 5 cut(s) 182, 507, 559, 630, 1233
HpyF3I CTNAG 6 cut(s) 134, 187, 199, 279, 549, 1374
HpySE526I ACGT 1 cut(s) 1032
Hsp92II CATG 8 cut(s) 501, 527, 567, 620, 653, 731, 744, 1304
HspAI GCGC 1 cut(s) 495
LmnI GCTCC 4 cut(s) 383, 586, 1099, 1266
Lsp1109I GCAGC 4 cut(s) 160, 190, 426, 539
LweI GCATC 1 cut(s) 156
MaeI CTAG 4 cut(s) 215, 1218, 1260, 1314
MaeII ACGT 1 cut(s) 1032
MaeIII GTNAC 2 cut(s) 223, 857
MfeI CAATTG 1 cut(s) 399
MflI RGATCY 3 cut(s) 130, 268, 1316
MhlI GDGCHC 1 cut(s) 591
MluCI AATT 7 cut(s) 44, 327, 399, 505, 658, 777, 800
MlyI GAGTC 1 cut(s) 191
MseI TTAA 5 cut(s) 47, 291, 504, 707, 1410
MslI CAYNNNNRTG 2 cut(s) 887, 1179
MspA1I CMGCKG 2 cut(s) 203, 341
MunI CAATTG 1 cut(s) 399
Mva1269I GAATGC 1 cut(s) 989
MwoI GCNNNNNNNGC 5 cut(s) 182, 507, 559, 630, 1233
NlaIII CATG 8 cut(s) 501, 527, 567, 620, 653, 731, 744, 1304
NlaIV GGNNCC 2 cut(s) 459, 1268
NmuCI GTSAC 1 cut(s) 223
NspI RCATGY 2 cut(s) 501, 527
PaeI GCATGC 2 cut(s) 501, 527
PctI GAATGC 1 cut(s) 989
PfeI GAWTC 5 cut(s) 65, 346, 719, 1083, 1276
PkrI GCNGC 5 cut(s) 175, 205, 265, 441, 529
PleI GAGTC 1 cut(s) 191
PpsI GAGTC 1 cut(s) 191
PspFI CCCAGC 2 cut(s) 337, 489
PspN4I GGNNCC 2 cut(s) 459, 1268
PspPI GGNCC 1 cut(s) 458
PsrI GAACNNNNNNTAC 2 cut(s) 1007, 1039
PstI CTGCAG 1 cut(s) 1294
PsuI RGATCY 3 cut(s) 130, 268, 1316
PvuII CAGCTG 2 cut(s) 203, 341
RsaI GTAC 4 cut(s) 99, 541, 674, 1423
RsaNI GTAC 4 cut(s) 98, 540, 673, 1422
RseI CAYNNNNRTG 2 cut(s) 887, 1179
SaqAI TTAA 5 cut(s) 47, 291, 504, 707, 1410
SatI GCNGC 5 cut(s) 174, 204, 264, 440, 528
Sau96I GGNCC 1 cut(s) 458
SchI GAGTC 1 cut(s) 191
SduI GDGCHC 1 cut(s) 591
SfaNI GCATC 1 cut(s) 156
SfcI CTRYAG 3 cut(s) 93, 387, 1290
SinI GGWCC 1 cut(s) 458
SmiMI CAYNNNNRTG 2 cut(s) 887, 1179
SmlI CTYRAG 1 cut(s) 770
SmoI CTYRAG 1 cut(s) 770
SphI GCATGC 2 cut(s) 501, 527
Sse9I AATT 7 cut(s) 44, 327, 399, 505, 658, 777, 800
SsiI CCGC 4 cut(s) 264, 442, 510, 553
SspI AATATT 1 cut(s) 433
SspMI CTAG 4 cut(s) 215, 1218, 1260, 1314
StyI CCWWGG 1 cut(s) 461
TaaI ACNGT 4 cut(s) 539, 544, 839, 925
TaiI ACGT 1 cut(s) 1035
TaqI TCGA 1 cut(s) 1286
TasI AATT 7 cut(s) 44, 327, 399, 505, 658, 777, 800
TatI WGTACW 2 cut(s) 97, 672
TauI GCSGC 1 cut(s) 266
TfiI GAWTC 5 cut(s) 65, 346, 719, 1083, 1276
Tru1I TTAA 5 cut(s) 47, 291, 504, 707, 1410
Tru9I TTAA 5 cut(s) 47, 291, 504, 707, 1410
TscAI CASTG 5 cut(s) 430, 537, 844, 889, 928
TseFI GTSAC 1 cut(s) 223
TseI GCWGC 4 cut(s) 173, 203, 439, 527
Tsp45I GTSAC 1 cut(s) 223
TspDTI ATGAA 4 cut(s) 23, 141, 729, 1373
TspGWI ACGGA 1 cut(s) 896
TspRI CASTG 5 cut(s) 430, 537, 844, 889, 928
VpaK11BI GGWCC 1 cut(s) 458
XagI CCTNNNNNAGG 1 cut(s) 1221
XapI RAATTY 2 cut(s) 777, 800
XbaI TCTAGA 1 cut(s) 1313
XceI RCATGY 2 cut(s) 501, 527
XcmI CCANNNNNNNNNTGG 1 cut(s) 486
XmiI GTMKAC 1 cut(s) 372
XspI CTAG 4 cut(s) 215, 1218, 1260, 1314
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.