MD03G1161300.v1.1

zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0005730
Physical Location & Seq
Reverse (-)
19445149 .. 19449363
4215 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1161300.v1.1.491

Sequence Viewer

Length: 1509 bp
ATGGGCAAGAGGGAGAGGCAAAAGGCCAAGCTTGATCGGGACGAAGTGAGCGAAGAAGAACGAGAATTAACTAATCAAAACCCTTCGAAATCTGCTGCCGAACAGAGCAGCAGCGACGACGATGAGGCCAACGAAGATATCAGCCTCAAAATCGTCGAGAAAGCTTTGTTAATGCGCGCGGCGAAGTTGGCATCCGATAACAACGCCGCCGTGTCGGGCGAGCCGAGTGGCGGTTTGGTTGAACTCCCATCTTCGTCTTCTTCGCAGGAAGCTGAAGTTGCGGCTGCAGGTTTGGAGACTAAGAAGGTAAGGAGGAAGCAGAGGAAGAAGAATATTAAGAAGATGAAGATTGAAGATCAAGCTGTTGTTGCAAAAGAGGAAGAGAAGATAGAGGAAGCTAATGCTGTAGATTTGGCTGAGGCTAAGCAACTGGAAAATGTTGAGTTTACCGACAATGTTGTGCTGCGGAAACTTCTACGGGGACCAAGGTATTTTGATCCTCCAGATAGTAGCTGGGGAGCATGCTTTAACTGTGGTGAAGAAGGTCATGCGGCAGTGAATTGTACCGTAGCTAAGCGAAAGAAGCCGTGCTTTGTTTGTGGCAGTTTGGAGCACAATGCAAAACACTGCAGAAAGGGACAAGATTGCTTTATCTGCAAAAAAGGTGGACACCATGCTAAAGACTGTCCAGAAAAGCAGAATGGTGGCTTATTGAAATCCCAAATATGTCTAAAATGTGGAGATTCTGGACATGATATGTTCTCATGCAGGAATGATTATTCAACTGATGATCTTAAGGAAATCCAATGCTATGTCTGCAAGAGATTTGGCCATTTATGTTGTGTCAAGTATGTTGACACTAGTCCATGGGAAGTTTCCTGTTACAAATGTGGTTCAATGGGGCATACTGGTATGGCATGTGTAAGCTTACGTGGTGGGGAAACCACTGGTTTTGGAACGCCTCGGTTATGCTACAAGTGTGGTGAAGGTGGTCATATTGCTCGTGAATGCGCAAGTTTGGTCAATGTTCACAAAAAAATTGGGGAATTACCTAATTTTTCTACTCCAGTTTTGAAACGTCATAAGGAAAGGAGAGACCACATGGGATTCAAGTCTGCTCCTCATGACCTAAATAAGGCCCATGAAAGGAAACAGACCCAATTTGAAGAAAGAGTTTTTACAACACCACAAAAGGCAAAACATAGAGGTGGTTGGATAATGGATGATCCAGGGGACTTCTCCCCTAGAAAAGGCAAAAAGAACAATTGGAAGTCTCCGGCAACACCATCTAGTTGGGGTCGTAGGGTTCCTAGTTTGACTGCAGGTGGTCATGCTTCAAGTTCTGGATCCTCTAAAAAGATTTGGAATGACTCCGGAAGCCCAATCTCACAAGGGTCGTCAAAGGCTTTTCAGCACAGATATTCTGCATCGAGGTTTAACAACTCTAATGGTGGTGGATTTAGAAGAAATTATGATTGGTCAGGCAATGAATATAATGGGCTTTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-CCHC

Protein Analysis

503

Amino Acids

55.55

Weight (kDa)

8.93

Isoelectric Point (pI)

48.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-CCHC PF00098 323 - 338 3.7e-06 Zinc knuckle
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1313
Acc16I TGCGCA 1 cut(s) 1012
Acc36I ACCTGC 2 cut(s) 278, 1313
AccII CGCG 2 cut(s) 177, 179
AccIII TCCGGA 1 cut(s) 1373
AciI CCGC 6 cut(s) 179, 207, 231, 281, 466, 551
AclWI GGATC 4 cut(s) 491, 1220, 1341, 1354
AcoI YGGCCR 1 cut(s) 829
AcuI CTGAAG 1 cut(s) 294
AfaI GTAC 1 cut(s) 565
AfiI CCNNNNNNNGG 4 cut(s) 230, 1135, 1146, 1250
AflII CTTAAG 1 cut(s) 794
AgsI TTSAA 9 cut(s) 242, 353, 715, 783, 897, 1075, 1111, 1166, 1338
AhlI ACTAGT 1 cut(s) 860
AjnI CCWGG 1 cut(s) 1228
AjuI GAANNNNNNNTTGG 2 cut(s) 1459, 1491
AluBI AGCT 8 cut(s) 31, 164, 272, 362, 398, 513, 572, 927
AluI AGCT 8 cut(s) 31, 164, 272, 362, 398, 513, 572, 927
Alw21I GWGCWC 1 cut(s) 615
Alw26I GTCTC 3 cut(s) 290, 1089, 1278
AlwI GGATC 4 cut(s) 491, 1220, 1341, 1354
Aor13HI TCCGGA 1 cut(s) 1373
AoxI GGCC 4 cut(s) 24, 126, 829, 1137
ApeKI GCWGC 5 cut(s) 95, 108, 111, 284, 463
AspLEI GCGC 3 cut(s) 177, 179, 1013
AspS9I GGNCC 2 cut(s) 482, 1138
AsuHPI GGTGA 2 cut(s) 548, 995
AsuII TTCGAA 1 cut(s) 86
AvaII GGWCC 1 cut(s) 482
BalI TGGCCA 1 cut(s) 831
BamHI GGATCC 1 cut(s) 1346
BauI CACGAG 1 cut(s) 1002
BbsI GAAGAC 1 cut(s) 249
Bbv12I GWGCWC 1 cut(s) 615
BbvCI CCTCAGC 1 cut(s) 417
BbvI GCAGC 5 cut(s) 82, 120, 123, 271, 450
BccI CCATC 2 cut(s) 256, 1294
BceAI ACGGC 2 cut(s) 194, 571
BciT130I CCWGG 1 cut(s) 1230
BcoDI GTCTC 3 cut(s) 290, 1089, 1278
BcuI ACTAGT 1 cut(s) 860
BfaI CTAG 4 cut(s) 861, 1245, 1290, 1311
BfmI CTRYAG 4 cut(s) 285, 405, 628, 1320
BfrI CTTAAG 1 cut(s) 794
BfuAI ACCTGC 2 cut(s) 278, 1313
BisI GCNGC 9 cut(s) 96, 109, 112, 180, 207, 282, 285, 464, 552
BlpI GCTNAGC 2 cut(s) 423, 573
BlsI GCNGC 9 cut(s) 97, 110, 113, 181, 208, 283, 286, 465, 553
Bme1390I CCNGG 1 cut(s) 1230
Bme18I GGWCC 1 cut(s) 482
BmgT120I GGNCC 2 cut(s) 482, 1138
BmiI GGNNCC 3 cut(s) 483, 1308, 1348
BmrFI CCNGG 1 cut(s) 1230
BmsI GCATC 2 cut(s) 200, 1436
BoxI GACNNNNGTC 1 cut(s) 861
BpiI GAAGAC 1 cut(s) 249
BpmI CTGGAG 2 cut(s) 486, 1050
Bpu10I CCTNAGC 1 cut(s) 417
Bpu1102I GCTNAGC 2 cut(s) 423, 573
Bpu14I TTCGAA 1 cut(s) 86
BsaAI YACGTR 1 cut(s) 932
BsaI GGTCTC 1 cut(s) 1089
BsaJI CCNNGG 4 cut(s) 485, 866, 962, 1229
BsaWI WCCGGW 1 cut(s) 1373
Bsc4I CCNNNNNNNGG 4 cut(s) 230, 1135, 1146, 1250
Bse1I ACTGG 4 cut(s) 435, 913, 952, 1067
Bse3DI GCAATG 1 cut(s) 1492
BseAI TCCGGA 1 cut(s) 1373
BseBI CCWGG 1 cut(s) 1230
BseDI CCNNGG 4 cut(s) 485, 866, 962, 1229
BseGI GGATG 2 cut(s) 191, 1228
BseLI CCNNNNNNNGG 4 cut(s) 230, 1135, 1146, 1250
BseMI GCAATG 1 cut(s) 1492
BseMII CTCAG 1 cut(s) 408
BseNI ACTGG 4 cut(s) 435, 913, 952, 1067
BsePI GCGCGC 1 cut(s) 175
BseRI GAGGAG 1 cut(s) 1110
BseXI GCAGC 5 cut(s) 82, 120, 123, 271, 450
BseYI CCCAGC 1 cut(s) 513
Bsh1236I CGCG 2 cut(s) 177, 179
BshFI GGCC 4 cut(s) 26, 128, 831, 1139
BsiHKAI GWGCWC 1 cut(s) 615
BsiSI CCGG 2 cut(s) 1277, 1374
BslFI GGGAC 4 cut(s) 53, 495, 651, 1247
BslI CCNNNNNNNGG 4 cut(s) 230, 1135, 1146, 1250
BsmAI GTCTC 3 cut(s) 290, 1089, 1278
BsmFI GGGAC 4 cut(s) 53, 495, 651, 1247
BsmI GAATGC 1 cut(s) 1013
BsnI GGCC 4 cut(s) 26, 128, 831, 1139
Bso31I GGTCTC 1 cut(s) 1089
Bsp119I TTCGAA 1 cut(s) 86
Bsp1286I GDGCHC 1 cut(s) 615
Bsp13I TCCGGA 1 cut(s) 1373
Bsp143I GATC 6 cut(s) 34, 355, 496, 790, 1225, 1346
Bsp1720I GCTNAGC 2 cut(s) 423, 573
Bsp19I CCATGG 1 cut(s) 866
BspACI CCGC 6 cut(s) 179, 207, 231, 281, 466, 551
BspANI GGCC 4 cut(s) 26, 128, 831, 1139
BspCNI CTCAG 1 cut(s) 409
BspEI TCCGGA 1 cut(s) 1373
BspFNI CGCG 2 cut(s) 177, 179
BspHI TCATGA 1 cut(s) 1123
BspLI GGNNCC 3 cut(s) 483, 1308, 1348
BspMAI CTGCAG 3 cut(s) 289, 632, 1324
BspMI ACCTGC 2 cut(s) 278, 1313
BspPI GGATC 4 cut(s) 491, 1220, 1341, 1354
BspT104I TTCGAA 1 cut(s) 86
BspTI CTTAAG 1 cut(s) 794
BspTNI GGTCTC 1 cut(s) 1089
BsrDI GCAATG 1 cut(s) 1492
BsrI ACTGG 4 cut(s) 435, 913, 952, 1067
BssECI CCNNGG 4 cut(s) 485, 866, 962, 1229
BssHII GCGCGC 1 cut(s) 175
BssMI GATC 6 cut(s) 34, 355, 496, 790, 1225, 1346
BssSI CACGAG 1 cut(s) 1002
BssT1I CCWWGG 2 cut(s) 485, 866
Bst2BI CACGAG 1 cut(s) 1002
Bst2UI CCWGG 1 cut(s) 1230
Bst4CI ACNGT 3 cut(s) 533, 568, 686
Bst6I CTCTTC 1 cut(s) 375
BstAFI CTTAAG 1 cut(s) 794
BstBAI YACGTR 1 cut(s) 932
BstBI TTCGAA 1 cut(s) 86
BstC8I GCNNGC 3 cut(s) 177, 221, 523
BstDEI CTNAG 4 cut(s) 300, 417, 423, 573
BstDSI CCRYGG 1 cut(s) 866
BstENI CCTNNNNNAGG 2 cut(s) 1133, 1248
BstF5I GGATG 2 cut(s) 191, 1228
BstFNI CGCG 2 cut(s) 177, 179
BstHHI GCGC 3 cut(s) 177, 179, 1013
BstKTI GATC 6 cut(s) 37, 358, 499, 793, 1228, 1349
BstMAI GTCTC 3 cut(s) 290, 1089, 1278
BstMBI GATC 6 cut(s) 34, 355, 496, 790, 1225, 1346
BstMWI GCNNNNNNNGC 6 cut(s) 188, 278, 368, 583, 654, 816
BstNI CCWGG 1 cut(s) 1230
BstNSI RCATGY 2 cut(s) 525, 921
BstPAI GACNNNNGTC 1 cut(s) 861
BstSCI CCNGG 1 cut(s) 1228
BstSFI CTRYAG 4 cut(s) 285, 405, 628, 1320
BstUI CGCG 2 cut(s) 177, 179
BstV1I GCAGC 5 cut(s) 82, 120, 123, 271, 450
BstV2I GAAGAC 1 cut(s) 249
BstX2I RGATCY 1 cut(s) 1346
BstXI CCANNNNNNTGG 1 cut(s) 1293
BstYI RGATCY 1 cut(s) 1346
BsuRI GGCC 4 cut(s) 26, 128, 831, 1139
BtgI CCRYGG 1 cut(s) 866
BtsCI GGATG 2 cut(s) 191, 1228
BtsI GCAGTG 2 cut(s) 561, 625
BtsIMutI CAGTG 3 cut(s) 561, 625, 945
BveI ACCTGC 2 cut(s) 278, 1313
Cac8I GCNNGC 3 cut(s) 177, 221, 523
CciI TCATGA 1 cut(s) 1123
CfoI GCGC 3 cut(s) 177, 179, 1013
Cfr13I GGNCC 2 cut(s) 482, 1138
Csp6I GTAC 1 cut(s) 564
CspCI CAANNNNNGTGG 4 cut(s) 646, 681, 934, 969
CviQI GTAC 1 cut(s) 564
DdeI CTNAG 4 cut(s) 300, 417, 423, 573
DpnI GATC 6 cut(s) 36, 357, 498, 792, 1227, 1348
DpnII GATC 6 cut(s) 34, 355, 496, 790, 1225, 1346
EaeI YGGCCR 1 cut(s) 829
Eam1104I CTCTTC 1 cut(s) 375
EarI CTCTTC 1 cut(s) 375
Eco130I CCWWGG 2 cut(s) 485, 866
Eco31I GGTCTC 1 cut(s) 1089
Eco32I GATATC 1 cut(s) 139
Eco47I GGWCC 1 cut(s) 482
Eco57I CTGAAG 1 cut(s) 294
EcoNI CCTNNNNNAGG 2 cut(s) 1133, 1248
EcoRII CCWGG 1 cut(s) 1228
EcoRV GATATC 1 cut(s) 139
EcoT14I CCWWGG 2 cut(s) 485, 866
ErhI CCWWGG 2 cut(s) 485, 866
FalI AAGNNNNNCTT 2 cut(s) 575, 607
FaqI GGGAC 4 cut(s) 53, 495, 651, 1247
Fnu4HI GCNGC 9 cut(s) 96, 109, 112, 180, 207, 282, 285, 464, 552
FokI GGATG 2 cut(s) 178, 1235
Fsp4HI GCNGC 9 cut(s) 96, 109, 112, 180, 207, 282, 285, 464, 552
FspBI CTAG 4 cut(s) 861, 1245, 1290, 1311
FspI TGCGCA 1 cut(s) 1012
GlaI GCGC 3 cut(s) 176, 178, 1012
GluI GCNGC 9 cut(s) 96, 109, 112, 180, 207, 282, 285, 464, 552
GsaI CCCAGC 1 cut(s) 517
GsuI CTGGAG 2 cut(s) 486, 1050
HaeIII GGCC 4 cut(s) 26, 128, 831, 1139
HapII CCGG 2 cut(s) 1277, 1374
HhaI GCGC 3 cut(s) 177, 179, 1013
Hin6I GCGC 3 cut(s) 175, 177, 1011
HinP1I GCGC 3 cut(s) 175, 177, 1011
HincII GTYRAC 1 cut(s) 856
HindII GTYRAC 1 cut(s) 856
HindIII AAGCTT 3 cut(s) 29, 162, 925
HinfI GANTC 3 cut(s) 743, 1107, 1370
HpaII CCGG 2 cut(s) 1277, 1374
HphI GGTGA 2 cut(s) 548, 995
Hpy166II GTNNAC 4 cut(s) 447, 668, 856, 1030
Hpy188I TCNGA 1 cut(s) 196
Hpy188III TCNNGA 9 cut(s) 38, 157, 503, 689, 747, 1004, 1124, 1344, 1374
Hpy8I GTNNAC 4 cut(s) 447, 668, 856, 1030
Hpy99I CGWCG 3 cut(s) 119, 122, 158
HpyAV CCTTC 4 cut(s) 93, 298, 536, 980
HpyCH4III ACNGT 3 cut(s) 533, 568, 686
HpyCH4IV ACGT 2 cut(s) 931, 1078
HpyCH4V TGCA 9 cut(s) 287, 371, 620, 630, 657, 768, 819, 1322, 1427
HpyF10VI GCNNNNNNNGC 6 cut(s) 188, 278, 368, 583, 654, 816
HpyF3I CTNAG 4 cut(s) 300, 417, 423, 573
HpySE526I ACGT 2 cut(s) 931, 1078
HspAI GCGC 3 cut(s) 175, 177, 1011
Kpn2I TCCGGA 1 cut(s) 1373
Kzo9I GATC 6 cut(s) 34, 355, 496, 790, 1225, 1346
LmnI GCTCC 3 cut(s) 518, 610, 1123
Lsp1109I GCAGC 5 cut(s) 82, 120, 123, 271, 450
LweI GCATC 2 cut(s) 200, 1436
MaeI CTAG 4 cut(s) 861, 1245, 1290, 1311
MaeII ACGT 2 cut(s) 931, 1078
MaeIII GTNAC 1 cut(s) 881
MalI GATC 6 cut(s) 36, 357, 498, 792, 1227, 1348
MboI GATC 6 cut(s) 34, 355, 496, 790, 1225, 1346
MfeI CAATTG 1 cut(s) 1264
MflI RGATCY 1 cut(s) 1346
MhlI GDGCHC 1 cut(s) 615
MlsI TGGCCA 1 cut(s) 831
MluCI AATT 8 cut(s) 65, 559, 1038, 1046, 1054, 1160, 1264, 1468
MluNI TGGCCA 1 cut(s) 831
MlyI GAGTC 1 cut(s) 1364
MmeI TCCRAC 1 cut(s) 1193
Mox20I TGGCCA 1 cut(s) 831
MroI TCCGGA 1 cut(s) 1373
MscI TGGCCA 1 cut(s) 831
MseI TTAA 6 cut(s) 68, 170, 336, 528, 795, 1437
MslI CAYNNNNRTG 1 cut(s) 1206
Msp20I TGGCCA 1 cut(s) 831
MspCI CTTAAG 1 cut(s) 794
MspI CCGG 2 cut(s) 1277, 1374
MspR9I CCNGG 1 cut(s) 1230
MunI CAATTG 1 cut(s) 1264
Mva1269I GAATGC 1 cut(s) 1013
MvaI CCWGG 1 cut(s) 1230
MvnI CGCG 2 cut(s) 177, 179
MwoI GCNNNNNNNGC 6 cut(s) 188, 278, 368, 583, 654, 816
NcoI CCATGG 1 cut(s) 866
NdeII GATC 6 cut(s) 34, 355, 496, 790, 1225, 1346
NlaIV GGNNCC 3 cut(s) 483, 1308, 1348
NmeAIII GCCGAG 1 cut(s) 249
NsbI TGCGCA 1 cut(s) 1012
NspI RCATGY 2 cut(s) 525, 921
NspV TTCGAA 1 cut(s) 86
PaeI GCATGC 1 cut(s) 525
PagI TCATGA 1 cut(s) 1123
PaqCI CACCTGC 1 cut(s) 1313
PauI GCGCGC 1 cut(s) 175
PcsI WCGNNNNNNNCGW 2 cut(s) 48, 221
PctI GAATGC 1 cut(s) 1013
PfeI GAWTC 2 cut(s) 743, 1107
PkrI GCNGC 9 cut(s) 97, 110, 113, 181, 208, 283, 286, 465, 553
PleI GAGTC 1 cut(s) 1364
PpsI GAGTC 1 cut(s) 1364
Ppu21I YACGTR 1 cut(s) 932
PshAI GACNNNNGTC 1 cut(s) 861
Psp6I CCWGG 1 cut(s) 1228
PspFI CCCAGC 1 cut(s) 513
PspGI CCWGG 1 cut(s) 1228
PspN4I GGNNCC 3 cut(s) 483, 1308, 1348
PspPI GGNCC 2 cut(s) 482, 1138
PstI CTGCAG 3 cut(s) 289, 632, 1324
PsuI RGATCY 1 cut(s) 1346
PteI GCGCGC 1 cut(s) 175
RsaI GTAC 1 cut(s) 565
RsaNI GTAC 1 cut(s) 564
RseI CAYNNNNRTG 1 cut(s) 1206
SaqAI TTAA 6 cut(s) 68, 170, 336, 528, 795, 1437
SatI GCNGC 9 cut(s) 96, 109, 112, 180, 207, 282, 285, 464, 552
Sau3AI GATC 6 cut(s) 34, 355, 496, 790, 1225, 1346
Sau96I GGNCC 2 cut(s) 482, 1138
SchI GAGTC 1 cut(s) 1364
ScrFI CCNGG 1 cut(s) 1230
SduI GDGCHC 1 cut(s) 615
SfaNI GCATC 2 cut(s) 200, 1436
SfcI CTRYAG 4 cut(s) 285, 405, 628, 1320
SfuI TTCGAA 1 cut(s) 86
SinI GGWCC 1 cut(s) 482
SmiMI CAYNNNNRTG 1 cut(s) 1206
SmlI CTYRAG 1 cut(s) 794
SmoI CTYRAG 1 cut(s) 794
SpeI ACTAGT 1 cut(s) 860
SphI GCATGC 1 cut(s) 525
Sse9I AATT 8 cut(s) 65, 559, 1038, 1046, 1054, 1160, 1264, 1468
SsiI CCGC 6 cut(s) 179, 207, 231, 281, 466, 551
SspI AATATT 1 cut(s) 334
SspMI CTAG 4 cut(s) 861, 1245, 1290, 1311
StyD4I CCNGG 1 cut(s) 1228
StyI CCWWGG 2 cut(s) 485, 866
TaaI ACNGT 3 cut(s) 533, 568, 686
TaiI ACGT 2 cut(s) 934, 1081
TaqI TCGA 3 cut(s) 86, 156, 1430
TasI AATT 8 cut(s) 65, 559, 1038, 1046, 1054, 1160, 1264, 1468
TauI GCSGC 4 cut(s) 182, 209, 284, 554
TfiI GAWTC 2 cut(s) 743, 1107
Tru1I TTAA 6 cut(s) 68, 170, 336, 528, 795, 1437
Tru9I TTAA 6 cut(s) 68, 170, 336, 528, 795, 1437
TscAI CASTG 3 cut(s) 561, 632, 952
TseI GCWGC 5 cut(s) 95, 108, 111, 284, 463
TspDTI ATGAA 3 cut(s) 359, 1158, 1503
TspRI CASTG 3 cut(s) 561, 632, 952
Vha464I CTTAAG 1 cut(s) 794
VpaK11BI GGWCC 1 cut(s) 482
XagI CCTNNNNNAGG 2 cut(s) 1133, 1248
XceI RCATGY 2 cut(s) 525, 921
XcmI CCANNNNNNNNNTGG 1 cut(s) 510
XspI CTAG 4 cut(s) 861, 1245, 1290, 1311
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.