Rh6DG064500

zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0005730
Physical Location & Seq
Reverse (-)
6623741 .. 6627040
3300 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG064500.1

Sequence Viewer

Length: 1470 bp
ATGGGCAAGAGGGAGAAGCAAGCAAACAAGTTTCATTGGGACGAATTAAGTGACGAAGAAGGAGAATTAGCAAATAGAAAGGCTTTGAAATCTGTGGTACAGATCAGCAGCGACGATGATGAGGCCAATGAAGATCTCAGCCTCAAAATAGTTGAAAAGGCTTTGCTGATGCGTGCAGCCAAGCTGGCCTCAGAGAATGACGCCGTTACTTCCGATCCAGGTGGTTTGATTGGACTGCCTTCTTCGTCTTCTTCACAGGTTGCTGAAGTTGAGGCTGCAGATTTGGAGACTAAGAAAGTAGTTAAGAAAGATAGGGTGAGGAAGACTAAAAAGAGGAAGATTCAAGACCCAGCTGTGATTCTTGCTAAAGAAGAAGTGAAGGTTGACGCGGCTAGAGCTGCAGATGAGACAATTGAACTGAACAATGTTCAGATCACTGACAATATGGTGCTGCGGAAACTTCTTCGGGGACCAAGGTATTTTGATCCTCCAGATAGGAGCTGGGGGACATGCTTTAATTGTGGTGAAGAAGGACATGCAGCAGTGAACTGTACCGTAGCTAGGCGGAAGAAGCCGTGCTTTGTTTGTGGTAGTTTGGAGCACAATGCCAAGCAATGTGAAAAGGGACAAGATTGCTTTATCTGCAAAAAAGGTGGACATCATGCTAAAAATTGTCCAGAGAAGTACAATGTTGGCTCATTGAATTCCAAAATATGTTTAAAATGTGGAGATTCTGGGCATGAGATGTATTCATGTAAGAATGATTACCCATCTGATGACCTCAAGGAAATCCAATGTTATGTCTGCAAGAGATTTGGTCATTTATGTTGTGTCAAGTGTGTTGATAACAGTCTAAAGGAAGTATCTTGTTACAAATGTGGTCAACTGGGACATACTGGTATGGCGTGCATAGGCTTACGTGGTGGGGAAACCACTGTTTCTGGATCCCCTCGTTTATGCTACAAGTGTGGTGAAGGTGGTCATATTGCTCGGGAATGCAGAACCTCCACTAATGCTCGTAAGAGGTATGGTGAGTTCTCTAACGTATCTACTCCTTTGAGACCTCATAAAGAAAAGAAAGACTACACGGGATTCTATTCTGCTCCTAATGATCTGAATAAGAATAAACGGAAACATACCTATTATGATGAAAGAGGTTTTGCAACTCCACAAAATGCAAAACATAGAGGTGGATGGATAGTGGATGATCCAGATGACTTCTCCCCTAGAAAAGGCAAGCAGAACGGCTGGAAGTCTCCTGCAACACCTTCTAGTAGGAGCCATAGGATTTCAAGTTCGCCTGCAGGCGGTCATGCTTCAAGTTCAAGATCCTCTAAAAAGTCGTGGAAGGTTCCTGGAGGAACTCCTATCTCACAAGGATCATCCAAGTCTGTTCAGCACAGATATTCCGCACGGAGGTTTAGCAACTCTGGTACTGATGGTGTTAGACGGAATAATGAGTGGTGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-CCHC

Protein Analysis

489

Amino Acids

54.16

Weight (kDa)

9.17

Isoelectric Point (pI)

43.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-CCHC PF00098 319 - 334 3.8e-06 Zinc knuckle
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 389
AciI CCGC 5 cut(s) 389, 454, 565, 1308, 1410
AclWI GGATC 7 cut(s) 209, 479, 939, 952, 1202, 1323, 1387
AcsI RAATTY 1 cut(s) 703
AcuI CTGAAG 1 cut(s) 285
AcyI GRCGYC 1 cut(s) 201
AfaI GTAC 4 cut(s) 99, 553, 686, 1435
AfiI CCNNNNNNNGG 4 cut(s) 561, 1232, 1307, 1416
AgsI TTSAA 8 cut(s) 88, 155, 344, 416, 703, 1293, 1320, 1326
AjnI CCWGG 2 cut(s) 217, 1354
AloI GAACNNNNNNTCC 2 cut(s) 1279, 1311
AluBI AGCT 5 cut(s) 184, 353, 398, 501, 560
AluI AGCT 5 cut(s) 184, 353, 398, 501, 560
Alw21I GWGCWC 1 cut(s) 603
Alw26I GTCTC 4 cut(s) 281, 401, 1054, 1260
AlwI GGATC 7 cut(s) 209, 479, 939, 952, 1202, 1323, 1387
Ama87I CYCGRG 1 cut(s) 990
AoxI GGCC 2 cut(s) 123, 186
ApeKI GCWGC 6 cut(s) 108, 176, 275, 398, 451, 539
ApoI RAATTY 1 cut(s) 703
AspS9I GGNCC 1 cut(s) 470
AsuHPI GGTGA 4 cut(s) 328, 536, 983, 1043
AvaI CYCGRG 1 cut(s) 990
AvaII GGWCC 1 cut(s) 470
BamHI GGATCC 1 cut(s) 944
BbsI GAAGAC 2 cut(s) 240, 329
Bbv12I GWGCWC 1 cut(s) 603
BbvI GCAGC 6 cut(s) 120, 188, 262, 385, 438, 551
BccI CCATC 3 cut(s) 778, 1188, 1433
BceAI ACGGC 3 cut(s) 188, 559, 1261
BciT130I CCWGG 2 cut(s) 219, 1356
BcoDI GTCTC 4 cut(s) 281, 401, 1054, 1260
BfaI CTAG 4 cut(s) 393, 561, 1227, 1272
BfmI CTRYAG 3 cut(s) 276, 399, 1302
BglI GCCNNNNNGGC 1 cut(s) 185
BglII AGATCT 1 cut(s) 133
BisI GCNGC 7 cut(s) 109, 177, 276, 390, 399, 452, 540
BlsI GCNGC 7 cut(s) 110, 178, 277, 391, 400, 453, 541
Bme1390I CCNGG 2 cut(s) 219, 1356
Bme18I GGWCC 1 cut(s) 470
BmeT110I CYCGRG 1 cut(s) 990
BmgT120I GGNCC 1 cut(s) 470
BmiI GGNNCC 4 cut(s) 471, 946, 1280, 1353
BmrFI CCNGG 2 cut(s) 219, 1356
BmrI ACTGGG 1 cut(s) 896
BmsI GCATC 1 cut(s) 159
BmuI ACTGGG 1 cut(s) 896
BpiI GAAGAC 2 cut(s) 240, 329
BpmI CTGGAG 2 cut(s) 474, 1377
BpuEI CTTGAG 1 cut(s) 767
BsaAI YACGTR 1 cut(s) 920
BsaHI GRCGYC 1 cut(s) 201
BsaI GGTCTC 1 cut(s) 1054
BsaJI CCNNGG 1 cut(s) 473
Bsc4I CCNNNNNNNGG 4 cut(s) 561, 1232, 1307, 1416
Bse1I ACTGG 2 cut(s) 891, 901
Bse3DI GCAATG 1 cut(s) 620
BseBI CCWGG 2 cut(s) 219, 1356
BseDI CCNNGG 1 cut(s) 473
BseGI GGATG 3 cut(s) 1199, 1210, 1382
BseLI CCNNNNNNNGG 4 cut(s) 561, 1232, 1307, 1416
BseMI GCAATG 1 cut(s) 620
BseMII CTCAG 2 cut(s) 151, 204
BseNI ACTGG 2 cut(s) 891, 901
BseXI GCAGC 6 cut(s) 120, 188, 262, 385, 438, 551
BseYI CCCAGC 2 cut(s) 349, 501
BsgI GTGCAG 1 cut(s) 195
Bsh1236I CGCG 1 cut(s) 389
BshFI GGCC 2 cut(s) 125, 188
BsiHKAI GWGCWC 1 cut(s) 603
BsiHKCI CYCGRG 1 cut(s) 990
BslFI GGGAC 5 cut(s) 53, 483, 520, 639, 903
BslI CCNNNNNNNGG 4 cut(s) 561, 1232, 1307, 1416
BsmAI GTCTC 4 cut(s) 281, 401, 1054, 1260
BsmFI GGGAC 5 cut(s) 53, 483, 520, 639, 903
BsmI GAATGC 1 cut(s) 1001
BsnI GGCC 2 cut(s) 125, 188
Bso31I GGTCTC 1 cut(s) 1054
BsoBI CYCGRG 1 cut(s) 990
Bsp1286I GDGCHC 1 cut(s) 603
BspACI CCGC 5 cut(s) 389, 454, 565, 1308, 1410
BspANI GGCC 2 cut(s) 125, 188
BspCNI CTCAG 2 cut(s) 150, 203
BspFNI CGCG 1 cut(s) 389
BspLI GGNNCC 4 cut(s) 471, 946, 1280, 1353
BspMAI CTGCAG 3 cut(s) 280, 403, 1306
BspPI GGATC 7 cut(s) 209, 479, 939, 952, 1202, 1323, 1387
BspTNI GGTCTC 1 cut(s) 1054
BsrDI GCAATG 1 cut(s) 620
BsrI ACTGG 2 cut(s) 891, 901
BssECI CCNNGG 1 cut(s) 473
BssNI GRCGYC 1 cut(s) 201
BssT1I CCWWGG 1 cut(s) 473
Bst2UI CCWGG 2 cut(s) 219, 1356
Bst4CI ACNGT 4 cut(s) 551, 556, 851, 937
BstACI GRCGYC 1 cut(s) 201
BstBAI YACGTR 1 cut(s) 920
BstC8I GCNNGC 7 cut(s) 21, 174, 186, 907, 1238, 1302, 1306
BstDEI CTNAG 3 cut(s) 137, 190, 291
BstENI CCTNNNNNAGG 1 cut(s) 1230
BstF5I GGATG 3 cut(s) 1199, 1210, 1382
BstFNI CGCG 1 cut(s) 389
BstMAI GTCTC 4 cut(s) 281, 401, 1054, 1260
BstMWI GCNNNNNNNGC 5 cut(s) 185, 395, 398, 571, 642
BstNI CCWGG 2 cut(s) 219, 1356
BstNSI RCATGY 2 cut(s) 513, 539
BstSCI CCNGG 2 cut(s) 217, 1354
BstSFI CTRYAG 3 cut(s) 276, 399, 1302
BstUI CGCG 1 cut(s) 389
BstV1I GCAGC 6 cut(s) 120, 188, 262, 385, 438, 551
BstV2I GAAGAC 2 cut(s) 240, 329
BstX2I RGATCY 3 cut(s) 133, 944, 1328
BstYI RGATCY 3 cut(s) 133, 944, 1328
BsuRI GGCC 2 cut(s) 125, 188
BtsCI GGATG 3 cut(s) 1199, 1210, 1382
BtsI GCAGTG 1 cut(s) 549
BtsIMutI CAGTG 3 cut(s) 435, 549, 933
Cac8I GCNNGC 7 cut(s) 21, 174, 186, 907, 1238, 1302, 1306
Cfr13I GGNCC 1 cut(s) 470
CseI GACGC 2 cut(s) 209, 395
Csp6I GTAC 4 cut(s) 98, 552, 685, 1434
CspCI CAANNNNNGTGG 2 cut(s) 634, 669
CviAII CATG 6 cut(s) 510, 536, 662, 740, 753, 1313
CviQI GTAC 4 cut(s) 98, 552, 685, 1434
DdeI CTNAG 3 cut(s) 137, 190, 291
DraI TTTAAA 1 cut(s) 720
EciI GGCGGA 1 cut(s) 580
Eco130I CCWWGG 1 cut(s) 473
Eco31I GGTCTC 1 cut(s) 1054
Eco47I GGWCC 1 cut(s) 470
Eco57I CTGAAG 1 cut(s) 285
Eco88I CYCGRG 1 cut(s) 990
EcoNI CCTNNNNNAGG 1 cut(s) 1230
EcoRI GAATTC 1 cut(s) 703
EcoRII CCWGG 2 cut(s) 217, 1354
EcoT14I CCWWGG 1 cut(s) 473
ErhI CCWWGG 1 cut(s) 473
FaeI CATG 6 cut(s) 513, 539, 665, 743, 756, 1316
FalI AAGNNNNNCTT 2 cut(s) 563, 595
FaqI GGGAC 5 cut(s) 53, 483, 520, 639, 903
FatI CATG 6 cut(s) 509, 535, 661, 739, 752, 1312
Fnu4HI GCNGC 7 cut(s) 109, 177, 276, 390, 399, 452, 540
FokI GGATG 3 cut(s) 1206, 1217, 1369
Fsp4HI GCNGC 7 cut(s) 109, 177, 276, 390, 399, 452, 540
FspBI CTAG 4 cut(s) 393, 561, 1227, 1272
GluI GCNGC 7 cut(s) 109, 177, 276, 390, 399, 452, 540
GsaI CCCAGC 2 cut(s) 353, 505
GsuI CTGGAG 2 cut(s) 474, 1377
HaeIII GGCC 2 cut(s) 125, 188
HgaI GACGC 2 cut(s) 209, 395
Hin1I GRCGYC 1 cut(s) 201
Hin1II CATG 6 cut(s) 513, 539, 665, 743, 756, 1316
HincII GTYRAC 2 cut(s) 385, 884
HindII GTYRAC 2 cut(s) 385, 884
HinfI GANTC 4 cut(s) 340, 358, 731, 1092
HphI GGTGA 4 cut(s) 328, 536, 983, 1043
Hpy166II GTNNAC 4 cut(s) 385, 547, 656, 884
Hpy188I TCNGA 5 cut(s) 193, 214, 432, 775, 1116
Hpy188III TCNNGA 7 cut(s) 344, 491, 677, 942, 992, 1211, 1326
Hpy8I GTNNAC 4 cut(s) 385, 547, 656, 884
Hpy99I CGWCG 1 cut(s) 116
HpyAV CCTTC 7 cut(s) 53, 249, 373, 524, 968, 1278, 1342
HpyCH4III ACNGT 4 cut(s) 551, 556, 851, 937
HpyCH4IV ACGT 2 cut(s) 919, 1044
HpyF10VI GCNNNNNNNGC 5 cut(s) 185, 395, 398, 571, 642
HpyF3I CTNAG 3 cut(s) 137, 190, 291
HpySE526I ACGT 2 cut(s) 919, 1044
Hsp92I GRCGYC 1 cut(s) 201
Hsp92II CATG 6 cut(s) 513, 539, 665, 743, 756, 1316
LmnI GCTCC 4 cut(s) 498, 598, 1108, 1278
Lsp1109I GCAGC 6 cut(s) 120, 188, 262, 385, 438, 551
LweI GCATC 1 cut(s) 159
MaeI CTAG 4 cut(s) 393, 561, 1227, 1272
MaeII ACGT 2 cut(s) 919, 1044
MaeIII GTNAC 3 cut(s) 50, 205, 869
MfeI CAATTG 1 cut(s) 411
MflI RGATCY 3 cut(s) 133, 944, 1328
MhlI GDGCHC 1 cut(s) 603
MluCI AATT 6 cut(s) 44, 65, 411, 517, 670, 703
MseI TTAA 4 cut(s) 47, 303, 516, 719
MslI CAYNNNNRTG 1 cut(s) 1188
MspA1I CMGCKG 1 cut(s) 353
MspR9I CCNGG 2 cut(s) 219, 1356
MunI CAATTG 1 cut(s) 411
Mva1269I GAATGC 1 cut(s) 1001
MvaI CCWGG 2 cut(s) 219, 1356
MvnI CGCG 1 cut(s) 389
MwoI GCNNNNNNNGC 5 cut(s) 185, 395, 398, 571, 642
NlaIII CATG 6 cut(s) 513, 539, 665, 743, 756, 1316
NlaIV GGNNCC 4 cut(s) 471, 946, 1280, 1353
NmuCI GTSAC 1 cut(s) 50
NspI RCATGY 2 cut(s) 513, 539
PctI GAATGC 1 cut(s) 1001
PfeI GAWTC 4 cut(s) 340, 358, 731, 1092
PfoI TCCNGGA 1 cut(s) 1354
PkrI GCNGC 7 cut(s) 110, 178, 277, 391, 400, 453, 541
Ppu21I YACGTR 1 cut(s) 920
Psp6I CCWGG 2 cut(s) 217, 1354
PspFI CCCAGC 2 cut(s) 349, 501
PspGI CCWGG 2 cut(s) 217, 1354
PspN4I GGNNCC 4 cut(s) 471, 946, 1280, 1353
PspPI GGNCC 1 cut(s) 470
PsrI GAACNNNNNNTAC 2 cut(s) 1019, 1051
PstI CTGCAG 3 cut(s) 280, 403, 1306
PsuI RGATCY 3 cut(s) 133, 944, 1328
PvuII CAGCTG 1 cut(s) 353
RsaI GTAC 4 cut(s) 99, 553, 686, 1435
RsaNI GTAC 4 cut(s) 98, 552, 685, 1434
RseI CAYNNNNRTG 1 cut(s) 1188
SaqAI TTAA 4 cut(s) 47, 303, 516, 719
SatI GCNGC 7 cut(s) 109, 177, 276, 390, 399, 452, 540
Sau96I GGNCC 1 cut(s) 470
SbfI CCTGCAGG 1 cut(s) 1306
ScrFI CCNGG 2 cut(s) 219, 1356
SdaI CCTGCAGG 1 cut(s) 1306
SduI GDGCHC 1 cut(s) 603
SfaNI GCATC 1 cut(s) 159
SfcI CTRYAG 3 cut(s) 276, 399, 1302
SinI GGWCC 1 cut(s) 470
SmiMI CAYNNNNRTG 1 cut(s) 1188
SmlI CTYRAG 1 cut(s) 782
SmoI CTYRAG 1 cut(s) 782
Sse8387I CCTGCAGG 1 cut(s) 1306
Sse9I AATT 6 cut(s) 44, 65, 411, 517, 670, 703
SsiI CCGC 5 cut(s) 389, 454, 565, 1308, 1410
SspMI CTAG 4 cut(s) 393, 561, 1227, 1272
StyD4I CCNGG 2 cut(s) 217, 1354
StyI CCWWGG 1 cut(s) 473
TaaI ACNGT 4 cut(s) 551, 556, 851, 937
TaiI ACGT 2 cut(s) 922, 1047
TasI AATT 6 cut(s) 44, 65, 411, 517, 670, 703
TatI WGTACW 1 cut(s) 684
TauI GCSGC 1 cut(s) 392
TfiI GAWTC 4 cut(s) 340, 358, 731, 1092
Tru1I TTAA 4 cut(s) 47, 303, 516, 719
Tru9I TTAA 4 cut(s) 47, 303, 516, 719
TscAI CASTG 3 cut(s) 442, 549, 940
TseFI GTSAC 1 cut(s) 50
TseI GCWGC 6 cut(s) 108, 176, 275, 398, 451, 539
Tsp45I GTSAC 1 cut(s) 50
TspDTI ATGAA 4 cut(s) 23, 144, 741, 1164
TspGWI ACGGA 3 cut(s) 1144, 1429, 1465
TspRI CASTG 3 cut(s) 442, 549, 940
VpaK11BI GGWCC 1 cut(s) 470
XagI CCTNNNNNAGG 1 cut(s) 1230
XapI RAATTY 1 cut(s) 703
XceI RCATGY 2 cut(s) 513, 539
XcmI CCANNNNNNNNNTGG 1 cut(s) 498
XspI CTAG 4 cut(s) 393, 561, 1227, 1272
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.