Rroxscaffold_7G00210030

zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0005730
Physical Location & Seq
Forward (+)
60317042 .. 60322225
5184 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00210030.1

Sequence Viewer

Length: 1467 bp
ATGGGCAAGAGGGAGAAGCAAGCGAACAAGTTTCACTGGGACGAGTTAAGTGAAGAAGAAGGAGAACTAGCTAATCGAAAGGCTCTGAAATCTGTCTTACACGTCAGTAGCGACGATGATGAGGCCAATGAAGATCTCAGCCTCAAAATAGTTGAAAAAGCGCTACTGATGCGTGCAGCCAAGCTGGCCTCAGAGAACGACACCGTTGTTTCCAATCCAGCTGGTTTGAATGGACTGCCTTCTTCGTCTTCTCCACGAGTTGCTGAAGTTGACGCCACAGATTTAGAGACTAAGAAAGTAAAGAAGGATAGGGTGAGGAAGACAAAAAAGAGGAAGATTGAAGACCCTGTTGTGATTCCTGCTAAAGAAGAAGTGAAGGTAGACGCAGCTAGAGCTGTGGATGAGCCAATTGAACTGAACACTGTTCAGATCACTGACAATATCGTGCTGCGGAAACTTCTTCGGGGACCAAGGTATTTTGATCCTCCAGATAGGAGCTGGGGAACATGCTTTAACTGTGGTGAAGAAGGACATGCAGCAGTGAACTGTACCGTAGCTAAGCGGAAGAAGCCATGCTTTGTTTGTGGCAGTTTGGAGCACAATGCCAAGCAATGTGAAAAGGGACAAGATTGCTTTATCTGCAAAAAAGGTGGACATCATGCTAAAAATTGTCCAGAGAAGTACAATGTTGGCTCATTGGATTCCAAAATATGTTTAAAATGTGGAGACTCTGGGCATGAGATGTATTCATGCAAGAATGATTACCCATCTGATGACCTCATGGAAATACAATGTTATGTCTGCAAGAGATTTGGTCATTTATGTTGTGTCAAGTGTGTTGATAATAGTCTAAGGGAAGTATCTTGTTACAAATGTGGTCAACTGGGACACACTGGTATGGCATGCATAGGCTTACGTGGTGGGGAAACCACTGTTTCTGGATCCCCTCGTTTATGCTACAAGTGTGGTGAAGGTGGTCATATTGCTCGGGAATGCAGAACTTCCAATAATGCTCGTAAGAGGTATGGCGAGTTCTCTAACGTACATACTCCTTTGAGACCTCATAAAGAAAAGAAAGACTACATGGGATTTTATTCTGCTCCTAATGATCTGGATAAGAATAAACGGAAACATACCCACTATGATGAAAGAGGTTTTGCAACTCCACAAAAAGCAAAACATAGAGGTGGATGGATAGTTGATGATCCAGATGACTTCTCCCCTAGAAAAGGCAAGCAGAACGGCTGGAAAGCTCCTGCAACACCATCTAGTAGGAGCCATAGGTTTTCAAGTTCGCCTGCAGTCGGTCATGCTTCGAGTTCTAGATCCTCCAAAAAGTTGTGGAAGGTTCCTGGAGGAACTCCTATCTCTCAAGGATCATCCAAGTCTGTTCAGCACAGATATTCTGCACGGAGGTTTAGCAACTCTGGTACTGATGGTGTTAGACGAAATAACGAGTGGTGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-CCHC

Protein Analysis

488

Amino Acids

54.36

Weight (kDa)

9.14

Isoelectric Point (pI)

47.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-CCHC PF00098 318 - 333 3.8e-06 Zinc knuckle
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 381
AciI CCGC 2 cut(s) 451, 562
AclWI GGATC 6 cut(s) 476, 936, 949, 1199, 1320, 1384
AcuI CTGAAG 1 cut(s) 285
AcyI GRCGYC 1 cut(s) 273
AfaI GTAC 4 cut(s) 550, 683, 1044, 1432
AfeI AGCGCT 1 cut(s) 162
AfiI CCNNNNNNNGG 2 cut(s) 1229, 1304
AflIII ACRYGT 1 cut(s) 100
AgsI TTSAA 5 cut(s) 155, 229, 341, 413, 1290
AjiI CACGTC 1 cut(s) 103
AjnI CCWGG 1 cut(s) 1351
AluBI AGCT 8 cut(s) 71, 184, 221, 389, 395, 498, 557, 1253
AluI AGCT 8 cut(s) 71, 184, 221, 389, 395, 498, 557, 1253
Alw21I GWGCWC 1 cut(s) 600
Alw26I GTCTC 3 cut(s) 281, 720, 1051
AlwI GGATC 6 cut(s) 476, 936, 949, 1199, 1320, 1384
Ama87I CYCGRG 1 cut(s) 987
Aor51HI AGCGCT 1 cut(s) 162
AoxI GGCC 2 cut(s) 123, 186
ApeKI GCWGC 4 cut(s) 176, 386, 448, 536
AspLEI GCGC 1 cut(s) 163
AspS9I GGNCC 1 cut(s) 467
AsuHPI GGTGA 3 cut(s) 325, 533, 980
AvaI CYCGRG 1 cut(s) 987
AvaII GGWCC 1 cut(s) 467
BamHI GGATCC 1 cut(s) 941
BauI CACGAG 1 cut(s) 255
BbsI GAAGAC 3 cut(s) 240, 326, 348
Bbv12I GWGCWC 1 cut(s) 600
BbvI GCAGC 4 cut(s) 188, 398, 435, 548
BccI CCATC 4 cut(s) 775, 1185, 1273, 1430
BceAI ACGGC 1 cut(s) 1258
BciT130I CCWGG 1 cut(s) 1353
BcoDI GTCTC 3 cut(s) 281, 720, 1051
BfaI CTAG 5 cut(s) 68, 390, 1224, 1269, 1323
BfmI CTRYAG 1 cut(s) 1299
BfoI RGCGCY 1 cut(s) 164
BglI GCCNNNNNGGC 1 cut(s) 185
BglII AGATCT 1 cut(s) 133
BisI GCNGC 4 cut(s) 177, 387, 449, 537
BlpI GCTNAGC 1 cut(s) 558
BlsI GCNGC 4 cut(s) 178, 388, 450, 538
Bme1390I CCNGG 1 cut(s) 1353
Bme18I GGWCC 1 cut(s) 467
BmeT110I CYCGRG 1 cut(s) 987
BmgBI CACGTC 1 cut(s) 103
BmgT120I GGNCC 1 cut(s) 467
BmiI GGNNCC 4 cut(s) 468, 943, 1277, 1350
BmrFI CCNGG 1 cut(s) 1353
BmrI ACTGGG 2 cut(s) 46, 893
BmsI GCATC 1 cut(s) 159
BmuI ACTGGG 2 cut(s) 46, 893
BpiI GAAGAC 3 cut(s) 240, 326, 348
BpmI CTGGAG 2 cut(s) 471, 1374
Bpu1102I GCTNAGC 1 cut(s) 558
BpuEI CTTGAG 1 cut(s) 1356
BsaAI YACGTR 1 cut(s) 917
BsaHI GRCGYC 1 cut(s) 273
BsaI GGTCTC 1 cut(s) 1051
BsaJI CCNNGG 1 cut(s) 470
Bsc4I CCNNNNNNNGG 2 cut(s) 1229, 1304
Bse1I ACTGG 3 cut(s) 41, 888, 898
Bse3DI GCAATG 1 cut(s) 617
BseBI CCWGG 1 cut(s) 1353
BseDI CCNNGG 1 cut(s) 470
BseGI GGATG 3 cut(s) 406, 1196, 1379
BseLI CCNNNNNNNGG 2 cut(s) 1229, 1304
BseMI GCAATG 1 cut(s) 617
BseMII CTCAG 2 cut(s) 151, 204
BseNI ACTGG 3 cut(s) 41, 888, 898
BseXI GCAGC 4 cut(s) 188, 398, 435, 548
BseYI CCCAGC 1 cut(s) 498
BsgI GTGCAG 2 cut(s) 195, 1392
BshFI GGCC 2 cut(s) 125, 188
BsiHKAI GWGCWC 1 cut(s) 600
BsiHKCI CYCGRG 1 cut(s) 987
BslFI GGGAC 4 cut(s) 53, 480, 636, 900
BslI CCNNNNNNNGG 2 cut(s) 1229, 1304
BsmAI GTCTC 3 cut(s) 281, 720, 1051
BsmFI GGGAC 4 cut(s) 53, 480, 636, 900
BsmI GAATGC 1 cut(s) 998
BsnI GGCC 2 cut(s) 125, 188
Bso31I GGTCTC 1 cut(s) 1051
BsoBI CYCGRG 1 cut(s) 987
Bsp1286I GDGCHC 1 cut(s) 600
Bsp143I GATC 8 cut(s) 133, 429, 481, 941, 1108, 1204, 1325, 1376
Bsp1720I GCTNAGC 1 cut(s) 558
BspACI CCGC 2 cut(s) 451, 562
BspANI GGCC 2 cut(s) 125, 188
BspCNI CTCAG 2 cut(s) 150, 203
BspLI GGNNCC 4 cut(s) 468, 943, 1277, 1350
BspMAI CTGCAG 1 cut(s) 1303
BspPI GGATC 6 cut(s) 476, 936, 949, 1199, 1320, 1384
BspTNI GGTCTC 1 cut(s) 1051
BsrDI GCAATG 1 cut(s) 617
BsrI ACTGG 3 cut(s) 41, 888, 898
BssECI CCNNGG 1 cut(s) 470
BssMI GATC 8 cut(s) 133, 429, 481, 941, 1108, 1204, 1325, 1376
BssNI GRCGYC 1 cut(s) 273
BssSI CACGAG 1 cut(s) 255
BssT1I CCWWGG 1 cut(s) 470
Bst2BI CACGAG 1 cut(s) 255
Bst2UI CCWGG 1 cut(s) 1353
Bst4CI ACNGT 6 cut(s) 205, 424, 518, 548, 553, 934
BstACI GRCGYC 1 cut(s) 273
BstBAI YACGTR 1 cut(s) 917
BstC8I GCNNGC 6 cut(s) 21, 174, 186, 904, 1235, 1299
BstDEI CTNAG 5 cut(s) 137, 190, 291, 558, 851
BstENI CCTNNNNNAGG 1 cut(s) 1227
BstF5I GGATG 3 cut(s) 406, 1196, 1379
BstH2I RGCGCY 1 cut(s) 164
BstHHI GCGC 1 cut(s) 163
BstKTI GATC 8 cut(s) 136, 432, 484, 944, 1111, 1207, 1328, 1379
BstMAI GTCTC 3 cut(s) 281, 720, 1051
BstMBI GATC 8 cut(s) 133, 429, 481, 941, 1108, 1204, 1325, 1376
BstMWI GCNNNNNNNGC 5 cut(s) 169, 185, 392, 568, 639
BstNI CCWGG 1 cut(s) 1353
BstNSI RCATGY 3 cut(s) 510, 536, 906
BstSCI CCNGG 1 cut(s) 1351
BstSFI CTRYAG 1 cut(s) 1299
BstV1I GCAGC 4 cut(s) 188, 398, 435, 548
BstV2I GAAGAC 3 cut(s) 240, 326, 348
BstX2I RGATCY 3 cut(s) 133, 941, 1325
BstYI RGATCY 3 cut(s) 133, 941, 1325
BsuRI GGCC 2 cut(s) 125, 188
BtrI CACGTC 1 cut(s) 103
BtsCI GGATG 3 cut(s) 406, 1196, 1379
BtsI GCAGTG 1 cut(s) 546
BtsIMutI CAGTG 6 cut(s) 34, 420, 432, 546, 891, 930
Cac8I GCNNGC 6 cut(s) 21, 174, 186, 904, 1235, 1299
CfoI GCGC 1 cut(s) 163
Cfr13I GGNCC 1 cut(s) 467
CseI GACGC 2 cut(s) 281, 392
Csp6I GTAC 4 cut(s) 549, 682, 1043, 1431
CspCI CAANNNNNGTGG 2 cut(s) 631, 666
CviQI GTAC 4 cut(s) 549, 682, 1043, 1431
DdeI CTNAG 5 cut(s) 137, 190, 291, 558, 851
DpnI GATC 8 cut(s) 135, 431, 483, 943, 1110, 1206, 1327, 1378
DpnII GATC 8 cut(s) 133, 429, 481, 941, 1108, 1204, 1325, 1376
DraI TTTAAA 1 cut(s) 717
Eco130I CCWWGG 1 cut(s) 470
Eco31I GGTCTC 1 cut(s) 1051
Eco47I GGWCC 1 cut(s) 467
Eco47III AGCGCT 1 cut(s) 162
Eco57I CTGAAG 1 cut(s) 285
Eco88I CYCGRG 1 cut(s) 987
EcoNI CCTNNNNNAGG 1 cut(s) 1227
EcoRII CCWGG 1 cut(s) 1351
EcoT14I CCWWGG 1 cut(s) 470
EcoT22I ATGCAT 1 cut(s) 908
ErhI CCWWGG 1 cut(s) 470
FalI AAGNNNNNCTT 2 cut(s) 560, 592
FaqI GGGAC 4 cut(s) 53, 480, 636, 900
FblI GTMKAC 1 cut(s) 381
Fnu4HI GCNGC 4 cut(s) 177, 387, 449, 537
FokI GGATG 3 cut(s) 413, 1203, 1366
Fsp4HI GCNGC 4 cut(s) 177, 387, 449, 537
FspBI CTAG 5 cut(s) 68, 390, 1224, 1269, 1323
GlaI GCGC 1 cut(s) 162
GluI GCNGC 4 cut(s) 177, 387, 449, 537
GsaI CCCAGC 1 cut(s) 502
GsuI CTGGAG 2 cut(s) 471, 1374
HaeII RGCGCY 1 cut(s) 164
HaeIII GGCC 2 cut(s) 125, 188
HgaI GACGC 2 cut(s) 281, 392
HhaI GCGC 1 cut(s) 163
Hin1I GRCGYC 1 cut(s) 273
Hin6I GCGC 1 cut(s) 161
HinP1I GCGC 1 cut(s) 161
HincII GTYRAC 2 cut(s) 271, 881
HindII GTYRAC 2 cut(s) 271, 881
HinfI GANTC 3 cut(s) 355, 701, 728
HphI GGTGA 3 cut(s) 325, 533, 980
Hpy166II GTNNAC 5 cut(s) 271, 382, 544, 653, 881
Hpy188I TCNGA 4 cut(s) 87, 193, 429, 772
Hpy188III TCNNGA 7 cut(s) 488, 674, 939, 989, 1112, 1208, 1323
Hpy8I GTNNAC 5 cut(s) 271, 382, 544, 653, 881
Hpy99I CGWCG 1 cut(s) 116
HpyAV CCTTC 7 cut(s) 53, 249, 298, 370, 521, 965, 1339
HpyCH4III ACNGT 6 cut(s) 205, 424, 518, 548, 553, 934
HpyCH4IV ACGT 3 cut(s) 102, 916, 1041
HpyF10VI GCNNNNNNNGC 5 cut(s) 169, 185, 392, 568, 639
HpyF3I CTNAG 5 cut(s) 137, 190, 291, 558, 851
HpySE526I ACGT 3 cut(s) 102, 916, 1041
Hsp92I GRCGYC 1 cut(s) 273
HspAI GCGC 1 cut(s) 161
Kzo9I GATC 8 cut(s) 133, 429, 481, 941, 1108, 1204, 1325, 1376
LmnI GCTCC 5 cut(s) 495, 595, 1105, 1258, 1275
Lsp1109I GCAGC 4 cut(s) 188, 398, 435, 548
LweI GCATC 1 cut(s) 159
MaeI CTAG 5 cut(s) 68, 390, 1224, 1269, 1323
MaeII ACGT 3 cut(s) 102, 916, 1041
MaeIII GTNAC 1 cut(s) 866
MalI GATC 8 cut(s) 135, 431, 483, 943, 1110, 1206, 1327, 1378
MboI GATC 8 cut(s) 133, 429, 481, 941, 1108, 1204, 1325, 1376
MfeI CAATTG 1 cut(s) 408
MflI RGATCY 3 cut(s) 133, 941, 1325
MhlI GDGCHC 1 cut(s) 600
MluCI AATT 2 cut(s) 408, 667
MlyI GAGTC 1 cut(s) 722
Mph1103I ATGCAT 1 cut(s) 908
MseI TTAA 3 cut(s) 47, 513, 716
MslI CAYNNNNRTG 3 cut(s) 896, 1143, 1185
MspA1I CMGCKG 1 cut(s) 221
MspR9I CCNGG 1 cut(s) 1353
MunI CAATTG 1 cut(s) 408
Mva1269I GAATGC 1 cut(s) 998
MvaI CCWGG 1 cut(s) 1353
MwoI GCNNNNNNNGC 5 cut(s) 169, 185, 392, 568, 639
NdeII GATC 8 cut(s) 133, 429, 481, 941, 1108, 1204, 1325, 1376
NlaIV GGNNCC 4 cut(s) 468, 943, 1277, 1350
NsiI ATGCAT 1 cut(s) 908
NspI RCATGY 3 cut(s) 510, 536, 906
PaeI GCATGC 1 cut(s) 906
PcsI WCGNNNNNNNCGW 1 cut(s) 108
PctI GAATGC 1 cut(s) 998
PfeI GAWTC 2 cut(s) 355, 701
PfoI TCCNGGA 1 cut(s) 1351
PkrI GCNGC 4 cut(s) 178, 388, 450, 538
PleI GAGTC 1 cut(s) 722
PpsI GAGTC 1 cut(s) 722
Ppu21I YACGTR 1 cut(s) 917
Psp6I CCWGG 1 cut(s) 1351
PspFI CCCAGC 1 cut(s) 498
PspGI CCWGG 1 cut(s) 1351
PspN4I GGNNCC 4 cut(s) 468, 943, 1277, 1350
PspPI GGNCC 1 cut(s) 467
PsrI GAACNNNNNNTAC 2 cut(s) 1016, 1048
PstI CTGCAG 1 cut(s) 1303
PsuI RGATCY 3 cut(s) 133, 941, 1325
PvuII CAGCTG 1 cut(s) 221
RsaI GTAC 4 cut(s) 550, 683, 1044, 1432
RsaNI GTAC 4 cut(s) 549, 682, 1043, 1431
RseI CAYNNNNRTG 3 cut(s) 896, 1143, 1185
SaqAI TTAA 3 cut(s) 47, 513, 716
SatI GCNGC 4 cut(s) 177, 387, 449, 537
Sau3AI GATC 8 cut(s) 133, 429, 481, 941, 1108, 1204, 1325, 1376
Sau96I GGNCC 1 cut(s) 467
SchI GAGTC 1 cut(s) 722
ScrFI CCNGG 1 cut(s) 1353
SduI GDGCHC 1 cut(s) 600
SfaNI GCATC 1 cut(s) 159
SfcI CTRYAG 1 cut(s) 1299
SinI GGWCC 1 cut(s) 467
SmiMI CAYNNNNRTG 3 cut(s) 896, 1143, 1185
SmlI CTYRAG 1 cut(s) 1371
SmoI CTYRAG 1 cut(s) 1371
SphI GCATGC 1 cut(s) 906
Sse9I AATT 2 cut(s) 408, 667
SsiI CCGC 2 cut(s) 451, 562
SspMI CTAG 5 cut(s) 68, 390, 1224, 1269, 1323
StyD4I CCNGG 1 cut(s) 1351
StyI CCWWGG 1 cut(s) 470
TaaI ACNGT 6 cut(s) 205, 424, 518, 548, 553, 934
TaiI ACGT 3 cut(s) 105, 919, 1044
TaqI TCGA 2 cut(s) 76, 1316
TaqII GACCGA 1 cut(s) 1295
TasI AATT 2 cut(s) 408, 667
TatI WGTACW 1 cut(s) 681
TfiI GAWTC 2 cut(s) 355, 701
Tru1I TTAA 3 cut(s) 47, 513, 716
Tru9I TTAA 3 cut(s) 47, 513, 716
TscAI CASTG 6 cut(s) 41, 427, 439, 546, 898, 937
TseI GCWGC 4 cut(s) 176, 386, 448, 536
TspDTI ATGAA 3 cut(s) 144, 738, 1161
TspGWI ACGGA 2 cut(s) 1141, 1426
TspRI CASTG 6 cut(s) 41, 427, 439, 546, 898, 937
VpaK11BI GGWCC 1 cut(s) 467
XagI CCTNNNNNAGG 1 cut(s) 1227
XbaI TCTAGA 1 cut(s) 1322
XceI RCATGY 3 cut(s) 510, 536, 906
XcmI CCANNNNNNNNNTGG 1 cut(s) 495
XmiI GTMKAC 1 cut(s) 381
XspI CTAG 5 cut(s) 68, 390, 1224, 1269, 1323
Zsp2I ATGCAT 1 cut(s) 908
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.