FvH4_2g05790

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0002668
Physical Location & Seq
Forward (+)
4735860 .. 4741156
5297 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g05790.t2

Sequence Viewer

Length: 1983 bp
ATGCAAGGTCAAGGGAGTGGCATTGATACTTTTTCAGAAATCACTGATGTTGGTCTCGGACCTGTTTCCAACAGTACTGGTGAACCTAGCTCTAGATTGAATTTGCAAAACACGGTCAATGATGGTGGGACAAGAATGGAACATGGTTGGTCGGCTCCACATAGTGCTTGTCCTGTGATTGCTCAAAGGCCAGAAGACAGACGAATTGAGCCAAATATCCTTTTTCCTGGGAGATCACTTATTGGCCTTGGTGGCAATCAGATTCCAGGTGGGCCTTCTTTTTCAGAAGGTTCTAGCTCTAATTATATCCCTCAGCATGTAAATTTAAATGCAGGGTATGTGGAAAATAGTGGTAATTATGGGCATGTTATGGAAGCTGATACAGATCCTAAATTCTACAAGTCAAGTGGATTAGGAGCAGAACATACATTTCCTGGCAGTGCTTCACATGATATTGTTGGGACTTCGTCCGGAAGCTCTGATTATATGGTTGGGGAAGATGATGGTAATCCAAGCTCTTCTTTTGGCAATTGGGGTTTATCCTGTAAGCGAAAGGCTCTTGAGGGTACGTCAGGGCAGTCTTATCCCGGTGGAAGTTCTAGCTCATTTCCTCAACCTGAAAATCAACCATGGAATACTGATCCTGCATGTTATAATCCTTCTAGTAGTTTAAGTTTATCTACGCCCCGTAGTTCTCCTAGTGGCATTCCTTCAGAACAGCAGAGCTCAAGAACGAGGATTGGTTTGAGAGTTGGAGCATCTGATGCCTTTCCTTCATTAGGTGTTACGGGAAATGCAGAGAGCCCACTGAGACAATATGTTAGGGGTTTCGGTAGTACTGGGTCTCAGCAAGAATCTGCACCATCAAACTTATCATCAGCCGGAAGATCAACTACAGTAGCTGCAACAAATTCGGGTGCTCCCCAAAGCCAGTCACCTGCAATGCATATGAACGGTTCATCGGGAAACATGCTTCCTTTCCCTTGGAATGGTGCTTCCAGTTCACGAGTGGGCAGCTTATCAGGCTCTCTTATGTCCGGATATATAGGTGCTGAGTTAAGAGAAGAAGCAAGCTCACGAAGCATTCCTAGAAACAATGTGGAGCACCCCATGTTTGTAGCTGCTACTGATATGAGAAATTCATCACAGGATCCAACAGGTTGGAGTTTAGCCCCCGGAGACTTGAGCACTTCTGTAGGTGCTCCTTCCTCTGCAAGAATTGGCTCCAATTCCAGCATTCATTCTTTGCCTAATCCCTCATGGATCCCTCTTCCAACAGAAAATCAAAGGATAACTGAATATGCACCTTGGTCTTTGTTTCCATCTATAGATTCTCAACCTGGAGGTCGTACTCATTATAATCCAATACCACCAGGACCTTCTGCTTCCACTCAGGATTCTCGAACTTCTTCTGGACCAAGTAATCAGGGTCGTCGACAACCACTTCATAGGTCAGCGTTCTTGTTGGATAGACAAGGTGATGATGTGCTTAATATGCCCCATTCGCTGCGTGCATTAGCAGCTGACAATGATGGGAGGCTCCGCCTTGTGTATGAGATTCGTCAAGTTTTGAATGCCATGCGCAGGGGCGAGAACTTACGAGTCGAGGATTATATGCTCTTTGACCCATATATTTATCATGGCATGGCTGAGATGCATGATAGGCACAGAGACATGCGGCTTGATGTTGACAACATGACTTACGAGGAGCTGTTAGCACTGGGGGAGCGTATAGGAGACGTTAGCACTGGACTGAGTGAGGAGACCATATTGAAATTGATGAAACAGAGAAAGTGTGTGTCTGTTATGACAGAATTCCCAGCAGACATGGAACCCTGCTGTATATGTCAGGAGCAATATGCTGATGGGGATGATATTGGCAGACTGGATTGTGGGCACGATTTCCACACCAACTGCATTAAACAGTGGCTAATGCAGAAGAATCTGTGCCCAATTTGTAAAACGCCAGCCTTGGTGACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000209 GO:0000902 GO:0000904 GO:0003006 GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006355 GO:0006357 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0006928 GO:0006935 GO:0007275 GO:0007399 GO:0007409 GO:0007411 GO:0007568 GO:0008045 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009605 GO:0009653 GO:0009791 GO:0009889 GO:0009966 GO:0009967 GO:0009987 GO:0010228 GO:0010259 GO:0010468 GO:0010498 GO:0010556 GO:0010646 GO:0010647 GO:0016043 GO:0016567 GO:0016740 GO:0019219 GO:0019222 GO:0019538 GO:0019787 GO:0019941 GO:0021675 GO:0022008 GO:0022414 GO:0023051 GO:0023056 GO:0030030 GO:0030154 GO:0030163 GO:0030182 GO:0030510 GO:0030513 GO:0031175 GO:0031323 GO:0031326 GO:0032446 GO:0032501 GO:0032502 GO:0032989 GO:0032990 GO:0032991 GO:0035107 GO:0035108 GO:0035136 GO:0036211 GO:0040011 GO:0042221 GO:0042330 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044464 GO:0048468 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048608 GO:0048666 GO:0048667 GO:0048699 GO:0048731 GO:0048736 GO:0048812 GO:0048856 GO:0048858 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0051603 GO:0060173 GO:0060255 GO:0060384 GO:0061061 GO:0061458 GO:0061564 GO:0061630 GO:0061659 GO:0065007 GO:0070647 GO:0071704 GO:0071840 GO:0080090 GO:0090092 GO:0090100 GO:0090287 GO:0097485 GO:0120036 GO:0120039 GO:0140096 GO:1901564 GO:1901565 GO:1901575 GO:1903506 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

661

Amino Acids

70.8

Weight (kDa)

5.42

Isoelectric Point (pI)

59.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 571 - 615 5.4e-09 RING-H2 zinc finger domain
zf-RING_2 PF13639 574 - 615 3.2e-12 Ring finger domain
zf-C3HC4_2 PF13923 574 - 614 1e-07 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 574 - 614 2.7e-06 Zinc finger, C3HC4 type (RING finger)
zf-RING_11 PF17123 574 - 601 6.9e-06 RING-like zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 654, 1359
AarI CACCTGC 1 cut(s) 946
Acc16I TGCGCA 1 cut(s) 1583
Acc36I ACCTGC 1 cut(s) 946
AccI GTMKAC 1 cut(s) 1435
AccIII TCCGGA 2 cut(s) 470, 1037
AciI CCGC 2 cut(s) 1543, 1678
AclWI GGATC 6 cut(s) 380, 635, 1145, 1158, 1258, 1271
AcsI RAATTY 6 cut(s) 100, 322, 392, 910, 1138, 1814
AcuI CTGAAG 1 cut(s) 696
AdeI CACNNNGTG 1 cut(s) 164
AfaI GTAC 4 cut(s) 76, 568, 838, 1351
AfiI CCNNNNNNNGG 3 cut(s) 779, 989, 1584
AgsI TTSAA 3 cut(s) 100, 1573, 1774
AjnI CCWGG 5 cut(s) 226, 265, 433, 1339, 1372
Alw21I GWGCWC 5 cut(s) 728, 922, 1107, 1190, 1204
Alw26I GTCTC 7 cut(s) 59, 805, 849, 1173, 1665, 1731, 1757
AlwI GGATC 6 cut(s) 380, 635, 1145, 1158, 1258, 1271
AlwNI CAGNNNCTG 1 cut(s) 902
Aor13HI TCCGGA 2 cut(s) 470, 1037
AoxI GGCC 3 cut(s) 188, 244, 272
ApeKI GCWGC 5 cut(s) 902, 1014, 1121, 1507, 1520
ApoI RAATTY 6 cut(s) 100, 322, 392, 910, 1138, 1814
Asp700I GAANNNNTTC 1 cut(s) 1408
AspLEI GCGC 1 cut(s) 1584
AspS9I GGNCC 4 cut(s) 59, 272, 1376, 1415
AsuC2I CCSGG 2 cut(s) 588, 1176
AsuHPI GGTGA 3 cut(s) 92, 927, 1490
AvaII GGWCC 3 cut(s) 59, 1376, 1415
BaeGI GKGCMC 2 cut(s) 1899, 1952
BamHI GGATCC 2 cut(s) 1150, 1263
BanII GRGCYC 2 cut(s) 728, 806
BarI GAAGNNNNNNTAC 2 cut(s) 877, 909
BauI CACGAG 1 cut(s) 1005
BbsI GAAGAC 1 cut(s) 201
Bbv12I GWGCWC 5 cut(s) 728, 922, 1107, 1190, 1204
BbvCI CCTCAGC 1 cut(s) 312
BbvI GCAGC 5 cut(s) 889, 1026, 1108, 1494, 1532
BccI CCATC 6 cut(s) 116, 497, 871, 1330, 1526, 1859
BcgI CGANNNNNNTGC 2 cut(s) 894, 928
BciT130I CCWGG 5 cut(s) 228, 267, 435, 1341, 1374
BcnI CCSGG 2 cut(s) 588, 1176
BcoDI GTCTC 7 cut(s) 59, 805, 849, 1173, 1665, 1731, 1757
BfaI CTAG 7 cut(s) 87, 93, 294, 600, 663, 699, 1089
BfmI CTRYAG 3 cut(s) 894, 1194, 1326
BfuAI ACCTGC 1 cut(s) 946
BglI GCCNNNNNGGC 1 cut(s) 252
BisI GCNGC 6 cut(s) 903, 1015, 1122, 1508, 1521, 1679
BlsI GCNGC 6 cut(s) 904, 1016, 1123, 1509, 1522, 1680
BmcAI AGTACT 2 cut(s) 76, 838
Bme1390I CCNGG 7 cut(s) 228, 267, 435, 588, 1176, 1341, 1374
Bme18I GGWCC 3 cut(s) 59, 1376, 1415
BmgT120I GGNCC 4 cut(s) 59, 272, 1376, 1415
BmiI GGNNCC 6 cut(s) 156, 1152, 1225, 1265, 1541, 1833
BmrFI CCNGG 7 cut(s) 228, 267, 435, 588, 1176, 1341, 1374
BmrI ACTGGG 2 cut(s) 849, 1730
BmsI GCATC 3 cut(s) 754, 767, 1644
BmuI ACTGGG 2 cut(s) 849, 1730
BpiI GAAGAC 1 cut(s) 201
BpmI CTGGAG 1 cut(s) 1362
Bpu10I CCTNAGC 1 cut(s) 312
BpuEI CTTGAG 3 cut(s) 581, 712, 1204
BpuMI CCSGG 2 cut(s) 588, 1176
BsaBI GATNNNNATC 2 cut(s) 384, 507
BsaI GGTCTC 3 cut(s) 59, 849, 1757
BsaJI CCNNGG 7 cut(s) 227, 247, 629, 983, 1174, 1307, 1971
BsaWI WCCGGW 2 cut(s) 470, 1037
Bsc4I CCNNNNNNNGG 3 cut(s) 779, 989, 1584
Bse1I ACTGG 7 cut(s) 82, 844, 931, 999, 1725, 1753, 1890
Bse3DI GCAATG 1 cut(s) 948
Bse8I GATNNNNATC 2 cut(s) 384, 507
BseAI TCCGGA 2 cut(s) 470, 1037
BseBI CCWGG 5 cut(s) 228, 267, 435, 1341, 1374
BseDI CCNNGG 7 cut(s) 227, 247, 629, 983, 1174, 1307, 1971
BseGI GGATG 1 cut(s) 1876
BseJI GATNNNNATC 2 cut(s) 384, 507
BseLI CCNNNNNNNGG 3 cut(s) 779, 989, 1584
BseMI GCAATG 1 cut(s) 948
BseMII CTCAG 7 cut(s) 326, 800, 860, 1044, 1406, 1641, 1745
BseNI ACTGG 7 cut(s) 82, 844, 931, 999, 1725, 1753, 1890
BseRI GAGGAG 2 cut(s) 1721, 1775
BseSI GKGCMC 2 cut(s) 1899, 1952
BseXI GCAGC 5 cut(s) 889, 1026, 1108, 1494, 1532
BseYI CCCAGC 1 cut(s) 1819
BsgI GTGCAG 1 cut(s) 843
BshFI GGCC 3 cut(s) 190, 246, 274
BsiHKAI GWGCWC 5 cut(s) 728, 922, 1107, 1190, 1204
BsiSI CCGG 5 cut(s) 471, 588, 882, 1038, 1176
BslFI GGGAC 2 cut(s) 142, 475
BslI CCNNNNNNNGG 3 cut(s) 779, 989, 1584
BsmAI GTCTC 7 cut(s) 59, 805, 849, 1173, 1665, 1731, 1757
BsmBI CGTCTC 1 cut(s) 1731
BsmFI GGGAC 2 cut(s) 142, 475
BsmI GAATGC 4 cut(s) 705, 1083, 1236, 1579
BsnI GGCC 3 cut(s) 190, 246, 274
Bso31I GGTCTC 3 cut(s) 59, 849, 1757
Bsp1286I GDGCHC 8 cut(s) 728, 806, 922, 1107, 1190, 1204, 1899, 1952
Bsp13I TCCGGA 2 cut(s) 470, 1037
Bsp143I GATC 6 cut(s) 233, 385, 640, 887, 1150, 1263
Bsp19I CCATGG 1 cut(s) 629
BspACI CCGC 2 cut(s) 1543, 1678
BspANI GGCC 3 cut(s) 190, 246, 274
BspCNI CTCAG 7 cut(s) 325, 801, 859, 1045, 1405, 1642, 1746
BspEI TCCGGA 2 cut(s) 470, 1037
BspLI GGNNCC 6 cut(s) 156, 1152, 1225, 1265, 1541, 1833
BspMI ACCTGC 1 cut(s) 946
BspPI GGATC 6 cut(s) 380, 635, 1145, 1158, 1258, 1271
BspQI GCTCTTC 1 cut(s) 523
BspTNI GGTCTC 3 cut(s) 59, 849, 1757
BsrDI GCAATG 1 cut(s) 948
BsrI ACTGG 7 cut(s) 82, 844, 931, 999, 1725, 1753, 1890
BssECI CCNNGG 7 cut(s) 227, 247, 629, 983, 1174, 1307, 1971
BssMI GATC 6 cut(s) 233, 385, 640, 887, 1150, 1263
BssSI CACGAG 1 cut(s) 1005
BssT1I CCWWGG 5 cut(s) 247, 629, 983, 1307, 1971
Bst2BI CACGAG 1 cut(s) 1005
Bst2UI CCWGG 5 cut(s) 228, 267, 435, 1341, 1374
Bst4CI ACNGT 5 cut(s) 74, 115, 898, 956, 1926
Bst6I CTCTTC 2 cut(s) 523, 1275
BstAPI GCANNNNNTGC 1 cut(s) 764
BstC8I GCNNGC 3 cut(s) 1072, 1512, 1968
BstDEI CTNAG 7 cut(s) 312, 809, 846, 1053, 1392, 1650, 1754
BstDSI CCRYGG 1 cut(s) 629
BstENI CCTNNNNNAGG 1 cut(s) 777
BstF5I GGATG 1 cut(s) 1876
BstHHI GCGC 1 cut(s) 1584
BstKTI GATC 6 cut(s) 236, 388, 643, 890, 1153, 1266
BstMAI GTCTC 7 cut(s) 59, 805, 849, 1173, 1665, 1731, 1757
BstMBI GATC 6 cut(s) 233, 385, 640, 887, 1150, 1263
BstMWI GCNNNNNNNGC 8 cut(s) 252, 764, 1023, 1080, 1495, 1504, 1520, 1663
BstNI CCWGG 5 cut(s) 228, 267, 435, 1341, 1374
BstNSI RCATGY 5 cut(s) 320, 368, 651, 973, 1678
BstSCI CCNGG 7 cut(s) 226, 265, 433, 586, 1174, 1339, 1372
BstSFI CTRYAG 3 cut(s) 894, 1194, 1326
BstSLI GKGCMC 2 cut(s) 1899, 1952
BstV1I GCAGC 5 cut(s) 889, 1026, 1108, 1494, 1532
BstV2I GAAGAC 1 cut(s) 201
BstX2I RGATCY 3 cut(s) 385, 1150, 1263
BstXI CCANNNNNNTGG 1 cut(s) 1161
BstYI RGATCY 3 cut(s) 385, 1150, 1263
BsuRI GGCC 3 cut(s) 190, 246, 274
BtgI CCRYGG 1 cut(s) 629
BtsCI GGATG 1 cut(s) 1876
BtsI GCAGTG 1 cut(s) 445
BtsIMutI CAGTG 6 cut(s) 42, 445, 806, 1718, 1746, 1931
BveI ACCTGC 1 cut(s) 946
Cac8I GCNNGC 3 cut(s) 1072, 1512, 1968
CaiI CAGNNNCTG 1 cut(s) 902
CfoI GCGC 1 cut(s) 1584
Cfr13I GGNCC 4 cut(s) 59, 272, 1376, 1415
Csp6I GTAC 4 cut(s) 75, 567, 837, 1350
CspCI CAANNNNNGTGG 4 cut(s) 106, 141, 388, 423
CviQI GTAC 4 cut(s) 75, 567, 837, 1350
DdeI CTNAG 7 cut(s) 312, 809, 846, 1053, 1392, 1650, 1754
DpnI GATC 6 cut(s) 235, 387, 642, 889, 1152, 1265
DpnII GATC 6 cut(s) 233, 385, 640, 887, 1150, 1263
DraI TTTAAA 1 cut(s) 327
DraIII CACNNNGTG 1 cut(s) 164
Eam1104I CTCTTC 2 cut(s) 523, 1275
EarI CTCTTC 2 cut(s) 523, 1275
EciI GGCGGA 1 cut(s) 1532
Ecl136II GAGCTC 1 cut(s) 726
Eco130I CCWWGG 5 cut(s) 247, 629, 983, 1307, 1971
Eco24I GRGCYC 2 cut(s) 728, 806
Eco31I GGTCTC 3 cut(s) 59, 849, 1757
Eco47I GGWCC 3 cut(s) 59, 1376, 1415
Eco53kI GAGCTC 1 cut(s) 726
Eco57I CTGAAG 1 cut(s) 696
EcoICRI GAGCTC 1 cut(s) 726
EcoNI CCTNNNNNAGG 1 cut(s) 777
EcoO109I RGGNCCY 1 cut(s) 1376
EcoRI GAATTC 1 cut(s) 1814
EcoRII CCWGG 5 cut(s) 226, 265, 433, 1339, 1372
EcoT14I CCWWGG 5 cut(s) 247, 629, 983, 1307, 1971
EcoT22I ATGCAT 2 cut(s) 948, 1659
EcoT38I GRGCYC 2 cut(s) 728, 806
ErhI CCWWGG 5 cut(s) 247, 629, 983, 1307, 1971
Esp3I CGTCTC 1 cut(s) 1731
FalI AAGNNNNNCTT 2 cut(s) 505, 537
FaqI GGGAC 2 cut(s) 142, 475
FauNDI CATATG 1 cut(s) 948
FblI GTMKAC 1 cut(s) 1435
Fnu4HI GCNGC 6 cut(s) 903, 1015, 1122, 1508, 1521, 1679
FokI GGATG 1 cut(s) 1883
FriOI GRGCYC 2 cut(s) 728, 806
Fsp4HI GCNGC 6 cut(s) 903, 1015, 1122, 1508, 1521, 1679
FspBI CTAG 7 cut(s) 87, 93, 294, 600, 663, 699, 1089
FspI TGCGCA 1 cut(s) 1583
GlaI GCGC 1 cut(s) 1583
GluI GCNGC 6 cut(s) 903, 1015, 1122, 1508, 1521, 1679
GsaI CCCAGC 1 cut(s) 1823
GsuI CTGGAG 1 cut(s) 1362
HaeIII GGCC 3 cut(s) 190, 246, 274
HapII CCGG 5 cut(s) 471, 588, 882, 1038, 1176
HhaI GCGC 1 cut(s) 1584
Hin6I GCGC 1 cut(s) 1582
HinP1I GCGC 1 cut(s) 1582
HincII GTYRAC 2 cut(s) 1436, 1690
HindII GTYRAC 2 cut(s) 1436, 1690
HinfI GANTC 7 cut(s) 262, 854, 1331, 1397, 1558, 1602, 1942
HpaII CCGG 5 cut(s) 471, 588, 882, 1038, 1176
HphI GGTGA 3 cut(s) 92, 927, 1490
Hpy166II GTNNAC 4 cut(s) 83, 1004, 1436, 1690
Hpy188I TCNGA 7 cut(s) 37, 59, 261, 286, 481, 715, 763
Hpy8I GTNNAC 4 cut(s) 83, 1004, 1436, 1690
Hpy99I CGWCG 1 cut(s) 1437
HpyAV CCTTC 7 cut(s) 281, 285, 669, 720, 783, 1215, 1389
HpyCH4III ACNGT 5 cut(s) 74, 115, 898, 956, 1926
HpyCH4IV ACGT 2 cut(s) 569, 1740
HpyF10VI GCNNNNNNNGC 8 cut(s) 252, 764, 1023, 1080, 1495, 1504, 1520, 1663
HpyF3I CTNAG 7 cut(s) 312, 809, 846, 1053, 1392, 1650, 1754
HpySE526I ACGT 2 cut(s) 569, 1740
HspAI GCGC 1 cut(s) 1582
Kpn2I TCCGGA 2 cut(s) 470, 1037
Kzo9I GATC 6 cut(s) 233, 385, 640, 887, 1150, 1263
LguI GCTCTTC 1 cut(s) 523
Lsp1109I GCAGC 5 cut(s) 889, 1026, 1108, 1494, 1532
LweI GCATC 3 cut(s) 754, 767, 1644
MaeI CTAG 7 cut(s) 87, 93, 294, 600, 663, 699, 1089
MaeII ACGT 2 cut(s) 569, 1740
MaeIII GTNAC 3 cut(s) 784, 933, 1975
MalI GATC 6 cut(s) 235, 387, 642, 889, 1152, 1265
MboI GATC 6 cut(s) 233, 385, 640, 887, 1150, 1263
MboII GAAGA 8 cut(s) 206, 509, 510, 897, 1076, 1262, 1401, 1951
MfeI CAATTG 1 cut(s) 529
MflI RGATCY 3 cut(s) 385, 1150, 1263
MhlI GDGCHC 8 cut(s) 728, 806, 922, 1107, 1190, 1204, 1899, 1952
MlyI GAGTC 1 cut(s) 1611
MmeI TCCRAC 6 cut(s) 93, 733, 1142, 1178, 1298, 1446
Mph1103I ATGCAT 2 cut(s) 948, 1659
MroI TCCGGA 2 cut(s) 470, 1037
MroXI GAANNNNTTC 1 cut(s) 1408
MseI TTAA 5 cut(s) 326, 671, 1058, 1491, 1920
MspA1I CMGCKG 1 cut(s) 1523
MspI CCGG 5 cut(s) 471, 588, 882, 1038, 1176
MspR9I CCNGG 7 cut(s) 228, 267, 435, 588, 1176, 1341, 1374
MunI CAATTG 1 cut(s) 529
Mva1269I GAATGC 4 cut(s) 705, 1083, 1236, 1579
MvaI CCWGG 5 cut(s) 228, 267, 435, 1341, 1374
MwoI GCNNNNNNNGC 8 cut(s) 252, 764, 1023, 1080, 1495, 1504, 1520, 1663
NciI CCSGG 2 cut(s) 588, 1176
NcoI CCATGG 1 cut(s) 629
NdeI CATATG 1 cut(s) 948
NdeII GATC 6 cut(s) 233, 385, 640, 887, 1150, 1263
NlaIV GGNNCC 6 cut(s) 156, 1152, 1225, 1265, 1541, 1833
NmuCI GTSAC 2 cut(s) 933, 1975
NsbI TGCGCA 1 cut(s) 1583
NsiI ATGCAT 2 cut(s) 948, 1659
NspI RCATGY 5 cut(s) 320, 368, 651, 973, 1678
PaqCI CACCTGC 1 cut(s) 946
PciSI GCTCTTC 1 cut(s) 523
PctI GAATGC 4 cut(s) 705, 1083, 1236, 1579
PdmI GAANNNNTTC 1 cut(s) 1408
PfeI GAWTC 6 cut(s) 262, 854, 1331, 1397, 1558, 1942
PflFI GACNNNGTC 1 cut(s) 466
PkrI GCNGC 6 cut(s) 904, 1016, 1123, 1509, 1522, 1680
PleI GAGTC 1 cut(s) 1610
PpsI GAGTC 1 cut(s) 1610
PpuMI RGGWCCY 1 cut(s) 1376
PsiI TTATAA 2 cut(s) 654, 1359
Psp124BI GAGCTC 1 cut(s) 728
Psp5II RGGWCCY 1 cut(s) 1376
Psp6I CCWGG 5 cut(s) 226, 265, 433, 1339, 1372
PspFI CCCAGC 1 cut(s) 1819
PspGI CCWGG 5 cut(s) 226, 265, 433, 1339, 1372
PspN4I GGNNCC 6 cut(s) 156, 1152, 1225, 1265, 1541, 1833
PspPI GGNCC 4 cut(s) 59, 272, 1376, 1415
PspPPI RGGWCCY 1 cut(s) 1376
PstNI CAGNNNCTG 1 cut(s) 902
PsuI RGATCY 3 cut(s) 385, 1150, 1263
PsyI GACNNNGTC 1 cut(s) 466
PvuII CAGCTG 1 cut(s) 1523
RsaI GTAC 4 cut(s) 76, 568, 838, 1351
RsaNI GTAC 4 cut(s) 75, 567, 837, 1350
SacI GAGCTC 1 cut(s) 728
SalI GTCGAC 1 cut(s) 1434
SapI GCTCTTC 1 cut(s) 523
SaqAI TTAA 5 cut(s) 326, 671, 1058, 1491, 1920
SatI GCNGC 6 cut(s) 903, 1015, 1122, 1508, 1521, 1679
Sau3AI GATC 6 cut(s) 233, 385, 640, 887, 1150, 1263
Sau96I GGNCC 4 cut(s) 59, 272, 1376, 1415
ScaI AGTACT 2 cut(s) 76, 838
SchI GAGTC 1 cut(s) 1611
ScrFI CCNGG 7 cut(s) 228, 267, 435, 588, 1176, 1341, 1374
SduI GDGCHC 8 cut(s) 728, 806, 922, 1107, 1190, 1204, 1899, 1952
SfaNI GCATC 3 cut(s) 754, 767, 1644
SfcI CTRYAG 3 cut(s) 894, 1194, 1326
SinI GGWCC 3 cut(s) 59, 1376, 1415
SmiI ATTTAAAT 1 cut(s) 327
SmlI CTYRAG 3 cut(s) 560, 727, 1183
SmoI CTYRAG 3 cut(s) 560, 727, 1183
SsiI CCGC 2 cut(s) 1543, 1678
SspMI CTAG 7 cut(s) 87, 93, 294, 600, 663, 699, 1089
SstI GAGCTC 1 cut(s) 728
StyD4I CCNGG 7 cut(s) 226, 265, 433, 586, 1174, 1339, 1372
StyI CCWWGG 5 cut(s) 247, 629, 983, 1307, 1971
SwaI ATTTAAAT 1 cut(s) 327
TaaI ACNGT 5 cut(s) 74, 115, 898, 956, 1926
TaiI ACGT 2 cut(s) 572, 1743
TaqI TCGA 3 cut(s) 1402, 1435, 1605
TatI WGTACW 2 cut(s) 74, 836
TauI GCSGC 1 cut(s) 1681
TfiI GAWTC 6 cut(s) 262, 854, 1331, 1397, 1558, 1942
Tru1I TTAA 5 cut(s) 326, 671, 1058, 1491, 1920
Tru9I TTAA 5 cut(s) 326, 671, 1058, 1491, 1920
TscAI CASTG 6 cut(s) 49, 445, 813, 1725, 1753, 1931
TseFI GTSAC 2 cut(s) 933, 1975
TseI GCWGC 5 cut(s) 902, 1014, 1121, 1507, 1520
Tsp45I GTSAC 2 cut(s) 933, 1975
TspDTI ATGAA 7 cut(s) 765, 948, 965, 1131, 1229, 1436, 1796
TspRI CASTG 6 cut(s) 49, 445, 813, 1725, 1753, 1931
Tth111I GACNNNGTC 1 cut(s) 466
VpaK11BI GGWCC 3 cut(s) 59, 1376, 1415
XagI CCTNNNNNAGG 1 cut(s) 777
XapI RAATTY 6 cut(s) 100, 322, 392, 910, 1138, 1814
XbaI TCTAGA 1 cut(s) 92
XceI RCATGY 5 cut(s) 320, 368, 651, 973, 1678
XcmI CCANNNNNNNNNTGG 1 cut(s) 1006
XmiI GTMKAC 1 cut(s) 1435
XmnI GAANNNNTTC 1 cut(s) 1408
XspI CTAG 7 cut(s) 87, 93, 294, 600, 663, 699, 1089
ZrmI AGTACT 2 cut(s) 76, 838
Zsp2I ATGCAT 2 cut(s) 948, 1659
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.