Rorug05G0566300

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0002668
Physical Location & Seq
Forward (+)
75953208 .. 75957032
3825 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0566300.1

Sequence Viewer

Length: 2319 bp
ATGCAGAAGCCTCCGCAGTCTCAAGATTTTGCTTTGAAAGAGACCTCGCCCAACATTGGAGCGGGGTCGGTCACAGGTGACAAGCTCTCATGCACCTATGACCTTGTTGAGCAGATGCAATACCTTTATGTCCGGGTGGTAAAAGCAAAGGATTTACCTGCAAAAGACGTAACTGGTAGTTGTGATCCCTATGTTGAAGTGAAACTTGGTAACTACAAGGGAGTCACTAAGCATTTTGAGAAGAAGTCTAACCCTGTATGGAATCAAGTGTTTGCTTTCTCCAAAGATCGAATTCAAGCTTCGATTTTGGAAGCTGTGGTGAAAGACAAGGATGTTGTGGTTGATGATTTCATGGGAAGGGTTATATTTGAACTAAATGACATACCGAAACGCATTCCACCTGACAGCCCTTTGGCACCACAGTGGTATAGGCTGGATGATCGAAAAGGTGATAGGGTGAAGGGAGAGCTGATGTTGGCAGTTTGGATGGGAACTCAGGCAGATGAAGCATTTCCTGATGCTTGGCATTCAGATGCTGCAACAGTTGGGCCCGAAGGTGTGAATAACATTCGATCGAAGGTGTATCTCTCCCCTAAGCTTTGGTATGTGAGGGTCAATGTAATTGAAGCTCAGGACTTGTTGCCTACTGACAAGAGTAGGTACCCCGAAGTTTTTGTGAAAGTTATCTATGGGAATCAGGCATTGAGGACTAGAATATCACAGAGTAAAAGTATCAATCCAATGTGGAATGAGGATTTGATGTTTGTCGCCGCTGAACCGTTTGAAGAACCTTTATTTTTGACTGTGGAAGATAGAGTGGGATCAGGCAAAGATGAAGTCTTGGGGAAGTGTGTCATTGCTTTACAGAATGTGCAGAGGAGGTTGGACCATAAACCTGTGAACACAAGGTGGTATAATCTAGAGAAGCATGCAATTGTAGATGGGGAACAGAAGAAGGAGATTAAGTTTGCAAGCAGGATTCATTTAAGGGTTTGTTTGGATGGCGGGTATCATGTTCTGGATGAGTCGACACACTACAGTAGTGATCTTAGGCCAACAGCAAAGCAGTTGTGGAAACCGAGCATTGGGATTTTGGAGCTAGGGGTTATCAGTGCAGTGGGACTGATGCCAATGAAGGCGAAAGATAGCCGAGGAACCACAGATGCTTATTGTGTAGCTAAATATGGGCAGAAATGGGTTCGAACAAGGACAATTGTGGACAGCCTGTCTCCCAAATGGAATGAGCAGTACACGTGGGAGGTTTTCGACCCGTGTACTGTCATTTCAATTGGGGTTTTTGACAATGGTCATATACATGGGGGTGATAAAGGGGGAAAGGATTCAAGAATTGGGAAAGTAAGGATTCGGCTATCTACACTTGAAGCTGACAGGGTTTACACACATTCCTATCCTCTTCTTGTCCTGCAGCCTTCCGGGGTGAAGAAAATGGGTGAAATTCAACTGGCAGTGAGGTTTACATGCTCATCTTTGGTTAATATGTTGCACATGTATTCACATCCTTTATTGCCAAAAATGCACTACATTCATCCATTGTCTGTAATTCAGCTAGATAGCTTGAGGCACCAGGCTATGCAGATTGTCTCAATGAGGCTCAATCGGGCTGAGCCACCACTGAGGAAAGAGGTGGTGGAGTATATGCTAGACGTTGATTCGCATATGTGGAGCATGAGAAGAAGCAAGGCTAATTTTTTCAGAATAATGGGAGTATTAAGTGGGGTGATTGCAGTAGGAAAATGGCTCGATCAAATCTGCAACTGGAAGAATCCTCTTACAACCATTTTGATTCATGTCCTTTTCATCATATTGGTTCTTTATCCTGAACTAATTCTCCCCACTATTTTTCTCTACCTGTTCCTGATCGGCATTTGGAACTTCAGATGGAGGCCAAGACACCCTCCTCACATGGACACCAGGCTATCTCATGCTGATGCTGCTCATCCTGATGAACGTGACGAAGAGTTTGACACATTCCCAACTTCACGGCCATCAGACATTGTTCGAATGAGGTATGATCGACTGAGAAGTATAGCAGGGAGGGTTCAGACTGTTGTTGGTGATATGGCAACTCAAGGCGAAAGGTTTCAGTCTCTTCTGAGCTGGAGAGACCCAAGAGCAACCACTTTGTTTGTTACTTTCTGTCTGATTCTTGCCATTGTTCTCTATGTGACTCCATTCCAAGTCGTGGCTCTTCTAGCAGGAATTTACATGTTAAGGCATCCCAGGTTCCGCCACAAGCTTCCTTCAGCGCCACTCAACTTCTTTAGAAGGTTGCCTGCAAGATCAGACAGTATGCTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000209 GO:0000902 GO:0000904 GO:0003006 GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006355 GO:0006357 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0006928 GO:0006935 GO:0007275 GO:0007399 GO:0007409 GO:0007411 GO:0007568 GO:0008045 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009605 GO:0009653 GO:0009791 GO:0009889 GO:0009966 GO:0009967 GO:0009987 GO:0010228 GO:0010259 GO:0010468 GO:0010498 GO:0010556 GO:0010646 GO:0010647 GO:0016043 GO:0016567 GO:0016740 GO:0019219 GO:0019222 GO:0019538 GO:0019787 GO:0019941 GO:0021675 GO:0022008 GO:0022414 GO:0023051 GO:0023056 GO:0030030 GO:0030154 GO:0030163 GO:0030182 GO:0030510 GO:0030513 GO:0031175 GO:0031323 GO:0031326 GO:0032446 GO:0032501 GO:0032502 GO:0032989 GO:0032990 GO:0032991 GO:0035107 GO:0035108 GO:0035136 GO:0036211 GO:0040011 GO:0042221 GO:0042330 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044464 GO:0048468 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048608 GO:0048666 GO:0048667 GO:0048699 GO:0048731 GO:0048736 GO:0048812 GO:0048856 GO:0048858 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0051603 GO:0060173 GO:0060255 GO:0060384 GO:0061061 GO:0061458 GO:0061564 GO:0061630 GO:0061659 GO:0065007 GO:0070647 GO:0071704 GO:0071840 GO:0080090 GO:0090092 GO:0090100 GO:0090287 GO:0097485 GO:0120036 GO:0120039 GO:0140096 GO:1901564 GO:1901565 GO:1901575 GO:1903506 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

772

Amino Acids

88.37

Weight (kDa)

9.18

Isoelectric Point (pI)

43.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 41 - 145 1.4e-21 C2 domain
C2 PF00168 202 - 307 4.4e-21 C2 domain
C2 PF00168 363 - 472 1.8e-21 C2 domain
Pex24p PF06398 589 - 752 8.6e-07 Integral peroxisomal membrane peroxin
PRT_C PF08372 617 - 772 5.3e-81 Plant phosphoribosyltransferase C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 166
Acc65I GGTACC 1 cut(s) 660
AccB1I GGYRCC 3 cut(s) 415, 660, 1581
AccB7I CCANNNNNTGG 1 cut(s) 2203
AccBSI CCGCTC 1 cut(s) 62
AccI GTMKAC 1 cut(s) 1028
AciI CCGC 5 cut(s) 14, 62, 771, 1005, 2248
AclWI GGATC 2 cut(s) 179, 829
AcoI YGGCCR 1 cut(s) 2003
AcsI RAATTY 3 cut(s) 291, 1455, 2220
AcuI CTGAAG 2 cut(s) 1879, 2247
AcvI CACGTG 1 cut(s) 1254
AdeI CACNNNGTG 1 cut(s) 909
AfaI GTAC 3 cut(s) 662, 1250, 1276
AfiI CCNNNNNNNGG 3 cut(s) 56, 1085, 2203
AflIII ACRYGT 3 cut(s) 1251, 1506, 2226
AhdI GACNNNNNGTC 1 cut(s) 1225
AjnI CCWGG 3 cut(s) 1584, 1931, 2240
AjuI GAANNNNNNNTTGG 2 cut(s) 189, 221
AleI CACNNNNGTG 1 cut(s) 421
AloI GAACNNNNNNTCC 2 cut(s) 1833, 1865
Alw26I GTCTC 6 cut(s) 24, 35, 1233, 1606, 2112, 2118
AlwI GGATC 2 cut(s) 179, 829
AlwNI CAGNNNCTG 1 cut(s) 536
AoxI GGCC 4 cut(s) 548, 1052, 1904, 2003
ApaI GGGCCC 1 cut(s) 552
ApeKI GCWGC 3 cut(s) 536, 1426, 1952
ApoI RAATTY 3 cut(s) 291, 1455, 2220
ArsI GACNNNNNNTTYG 4 cut(s) 822, 854, 1964, 1996
Asp700I GAANNNNTTC 4 cut(s) 510, 1339, 1845, 2100
Asp718I GGTACC 1 cut(s) 660
AspLEI GCGC 1 cut(s) 2269
AspS9I GGNCC 3 cut(s) 548, 549, 886
AsuC2I CCSGG 2 cut(s) 134, 1435
AsuHPI GGTGA 9 cut(s) 89, 331, 461, 469, 1334, 1450, 1463, 1750, 2087
AsuII TTCGAA 2 cut(s) 1201, 2020
AvaII GGWCC 1 cut(s) 886
BaeGI GKGCMC 1 cut(s) 552
BanI GGYRCC 3 cut(s) 415, 660, 1581
BanII GRGCYC 1 cut(s) 552
BbrPI CACGTG 1 cut(s) 1254
BbvI GCAGC 3 cut(s) 523, 1438, 1939
BccI CCATC 5 cut(s) 481, 935, 995, 1893, 2014
BceAI ACGGC 1 cut(s) 2018
BciT130I CCWGG 3 cut(s) 1586, 1933, 2242
BcnI CCSGG 2 cut(s) 134, 1435
BcoDI GTCTC 6 cut(s) 24, 35, 1233, 1606, 2112, 2118
BfaI CTAG 6 cut(s) 711, 920, 1100, 1568, 1661, 2213
BfmI CTRYAG 2 cut(s) 1036, 1424
BfoI RGCGCY 1 cut(s) 2270
BfuAI ACCTGC 1 cut(s) 166
BisI GCNGC 4 cut(s) 537, 771, 1427, 1953
BlpI GCTNAGC 1 cut(s) 1623
BlsI GCNGC 4 cut(s) 538, 772, 1428, 1954
Bme1390I CCNGG 5 cut(s) 134, 1435, 1586, 1933, 2242
Bme18I GGWCC 1 cut(s) 886
BmeRI GACNNNNNGTC 1 cut(s) 1225
BmgT120I GGNCC 3 cut(s) 548, 549, 886
BmiI GGNNCC 6 cut(s) 417, 550, 662, 1156, 1583, 2246
BmrFI CCNGG 5 cut(s) 134, 1435, 1586, 1933, 2242
BmsI GCATC 7 cut(s) 105, 508, 523, 1116, 1153, 1939, 2245
BoxI GACNNNNGTC 1 cut(s) 1305
BpmI CTGGAG 1 cut(s) 2140
Bpu10I CCTNAGC 2 cut(s) 594, 630
Bpu1102I GCTNAGC 1 cut(s) 1623
Bpu14I TTCGAA 2 cut(s) 1201, 2020
BpuEI CTTGAG 3 cut(s) 6, 1597, 2073
BpuMI CCSGG 2 cut(s) 134, 1435
BsaAI YACGTR 1 cut(s) 1254
BsaI GGTCTC 2 cut(s) 35, 2118
BsaJI CCNNGG 3 cut(s) 1150, 1434, 2240
BsaXI ACNNNNNCTCC 4 cut(s) 950, 980, 1833, 1863
Bsc4I CCNNNNNNNGG 3 cut(s) 56, 1085, 2203
Bse1I ACTGG 3 cut(s) 178, 1467, 1781
Bse3DI GCAATG 1 cut(s) 855
BseBI CCWGG 3 cut(s) 1586, 1933, 2242
BseDI CCNNGG 3 cut(s) 1150, 1434, 2240
BseGI GGATG 9 cut(s) 337, 442, 492, 1006, 1027, 1516, 1546, 1957, 2236
BseLI CCNNNNNNNGG 3 cut(s) 56, 1085, 2203
BseMI GCAATG 1 cut(s) 855
BseMII CTCAG 6 cut(s) 509, 644, 1614, 1625, 2030, 2105
BseNI ACTGG 3 cut(s) 178, 1467, 1781
BseRI GAGGAG 2 cut(s) 892, 1908
BseSI GKGCMC 1 cut(s) 552
BseXI GCAGC 3 cut(s) 523, 1438, 1939
BsgI GTGCAG 2 cut(s) 893, 1134
Bsh1285I CGRYCG 1 cut(s) 575
BshFI GGCC 4 cut(s) 550, 1054, 1906, 2005
BshNI GGYRCC 3 cut(s) 415, 660, 1581
BsiEI CGRYCG 1 cut(s) 575
BsiSI CCGG 2 cut(s) 133, 1434
BslFI GGGAC 1 cut(s) 1134
BslI CCNNNNNNNGG 3 cut(s) 56, 1085, 2203
BsmAI GTCTC 6 cut(s) 24, 35, 1233, 1606, 2112, 2118
BsmFI GGGAC 1 cut(s) 1134
BsmI GAATGC 2 cut(s) 393, 526
BsnI GGCC 4 cut(s) 550, 1054, 1906, 2005
Bso31I GGTCTC 2 cut(s) 35, 2118
Bsp119I TTCGAA 2 cut(s) 1201, 2020
Bsp120I GGGCCC 1 cut(s) 548
Bsp1286I GDGCHC 1 cut(s) 552
Bsp1720I GCTNAGC 1 cut(s) 1623
BspACI CCGC 5 cut(s) 14, 62, 771, 1005, 2248
BspANI GGCC 4 cut(s) 550, 1054, 1906, 2005
BspCNI CTCAG 6 cut(s) 508, 643, 1615, 1626, 2031, 2106
BspLI GGNNCC 6 cut(s) 417, 550, 662, 1156, 1583, 2246
BspMAI CTGCAG 1 cut(s) 1428
BspMI ACCTGC 1 cut(s) 166
BspPI GGATC 2 cut(s) 179, 829
BspQI GCTCTTC 1 cut(s) 2214
BspT104I TTCGAA 2 cut(s) 1201, 2020
BspT107I GGYRCC 3 cut(s) 415, 660, 1581
BspTNI GGTCTC 2 cut(s) 35, 2118
BsrBI CCGCTC 1 cut(s) 62
BsrDI GCAATG 1 cut(s) 855
BsrI ACTGG 3 cut(s) 178, 1467, 1781
BssECI CCNNGG 3 cut(s) 1150, 1434, 2240
Bst2UI CCWGG 3 cut(s) 1586, 1933, 2242
Bst4CI ACNGT 8 cut(s) 423, 544, 780, 805, 1040, 1279, 2068, 2309
Bst6I CTCTTC 4 cut(s) 1419, 1971, 2115, 2214
BstBAI YACGTR 1 cut(s) 1254
BstBI TTCGAA 2 cut(s) 1201, 2020
BstC8I GCNNGC 3 cut(s) 930, 973, 2295
BstDEI CTNAG 9 cut(s) 228, 495, 594, 630, 1049, 1623, 1634, 2039, 2114
BstF5I GGATG 9 cut(s) 337, 442, 492, 1006, 1027, 1516, 1546, 1957, 2236
BstH2I RGCGCY 1 cut(s) 2270
BstHHI GCGC 1 cut(s) 2269
BstMAI GTCTC 6 cut(s) 24, 35, 1233, 1606, 2112, 2118
BstMCI CGRYCG 1 cut(s) 575
BstMWI GCNNNNNNNGC 4 cut(s) 506, 1534, 1952, 2213
BstNI CCWGG 3 cut(s) 1586, 1933, 2242
BstNSI RCATGY 4 cut(s) 932, 1482, 1510, 2230
BstPAI GACNNNNGTC 1 cut(s) 1305
BstSCI CCNGG 5 cut(s) 132, 1433, 1584, 1931, 2240
BstSFI CTRYAG 2 cut(s) 1036, 1424
BstSLI GKGCMC 1 cut(s) 552
BstV1I GCAGC 3 cut(s) 523, 1438, 1939
BsuRI GGCC 4 cut(s) 550, 1054, 1906, 2005
BtsCI GGATG 9 cut(s) 337, 442, 492, 1006, 1027, 1516, 1546, 1957, 2236
BtsI GCAGTG 2 cut(s) 1122, 1473
BtsIMutI CAGTG 5 cut(s) 428, 1117, 1122, 1473, 1631
BveI ACCTGC 1 cut(s) 166
Cac8I GCNNGC 3 cut(s) 930, 973, 2295
CaiI CAGNNNCTG 1 cut(s) 536
CfoI GCGC 1 cut(s) 2269
Cfr13I GGNCC 3 cut(s) 548, 549, 886
Csp6I GTAC 3 cut(s) 661, 1249, 1275
CspCI CAANNNNNGTGG 2 cut(s) 2128, 2163
CviQI GTAC 3 cut(s) 661, 1249, 1275
DdeI CTNAG 9 cut(s) 228, 495, 594, 630, 1049, 1623, 1634, 2039, 2114
DraIII CACNNNGTG 1 cut(s) 909
DriI GACNNNNNGTC 1 cut(s) 1225
EaeI YGGCCR 1 cut(s) 2003
Eam1104I CTCTTC 4 cut(s) 1419, 1971, 2115, 2214
Eam1105I GACNNNNNGTC 1 cut(s) 1225
EarI CTCTTC 4 cut(s) 1419, 1971, 2115, 2214
EciI GGCGGA 1 cut(s) 2237
Eco24I GRGCYC 1 cut(s) 552
Eco31I GGTCTC 2 cut(s) 35, 2118
Eco47I GGWCC 1 cut(s) 886
Eco57I CTGAAG 2 cut(s) 1879, 2247
Eco72I CACGTG 1 cut(s) 1254
EcoRI GAATTC 1 cut(s) 291
EcoRII CCWGG 3 cut(s) 1584, 1931, 2240
EcoT38I GRGCYC 1 cut(s) 552
FalI AAGNNNNNCTT 2 cut(s) 189, 221
FaqI GGGAC 1 cut(s) 1134
FauI CCCGC 2 cut(s) 55, 998
FauNDI CATATG 1 cut(s) 1677
FblI GTMKAC 1 cut(s) 1028
Fnu4HI GCNGC 4 cut(s) 537, 771, 1427, 1953
FokI GGATG 9 cut(s) 344, 449, 499, 1013, 1034, 1503, 1533, 1944, 2223
FriOI GRGCYC 1 cut(s) 552
Fsp4HI GCNGC 4 cut(s) 537, 771, 1427, 1953
FspBI CTAG 6 cut(s) 711, 920, 1100, 1568, 1661, 2213
GlaI GCGC 1 cut(s) 2268
GluI GCNGC 4 cut(s) 537, 771, 1427, 1953
GsuI CTGGAG 1 cut(s) 2140
HaeII RGCGCY 1 cut(s) 2270
HaeIII GGCC 4 cut(s) 550, 1054, 1906, 2005
HapII CCGG 2 cut(s) 133, 1434
HhaI GCGC 1 cut(s) 2269
Hin6I GCGC 1 cut(s) 2267
HinP1I GCGC 1 cut(s) 2267
HincII GTYRAC 1 cut(s) 1029
HindII GTYRAC 1 cut(s) 1029
HindIII AAGCTT 3 cut(s) 297, 596, 2255
HpaII CCGG 2 cut(s) 133, 1434
HphI GGTGA 9 cut(s) 89, 331, 461, 469, 1334, 1450, 1463, 1750, 2087
Hpy166II GTNNAC 7 cut(s) 901, 1029, 1219, 1251, 1275, 1396, 1476
Hpy188I TCNGA 8 cut(s) 532, 1715, 1898, 2011, 2064, 2115, 2163, 2305
Hpy188III TCNNGA 9 cut(s) 23, 515, 632, 920, 1019, 1344, 1838, 1876, 1961
Hpy8I GTNNAC 7 cut(s) 901, 1029, 1219, 1251, 1275, 1396, 1476
HpyAV CCTTC 9 cut(s) 351, 454, 548, 571, 949, 1129, 1440, 2271, 2280
HpyCH4III ACNGT 8 cut(s) 423, 544, 780, 805, 1040, 1279, 2068, 2309
HpyCH4IV ACGT 4 cut(s) 168, 1253, 1665, 1969
HpyF10VI GCNNNNNNNGC 4 cut(s) 506, 1534, 1952, 2213
HpyF3I CTNAG 9 cut(s) 228, 495, 594, 630, 1049, 1623, 1634, 2039, 2114
HpySE526I ACGT 4 cut(s) 168, 1253, 1665, 1969
HspAI GCGC 1 cut(s) 2267
KpnI GGTACC 1 cut(s) 664
LguI GCTCTTC 1 cut(s) 2214
LmnI GCTCC 3 cut(s) 59, 1096, 1683
Lsp1109I GCAGC 3 cut(s) 523, 1438, 1939
LweI GCATC 7 cut(s) 105, 508, 523, 1116, 1153, 1939, 2245
MaeI CTAG 6 cut(s) 711, 920, 1100, 1568, 1661, 2213
MaeII ACGT 4 cut(s) 168, 1253, 1665, 1969
MaeIII GTNAC 8 cut(s) 70, 77, 169, 209, 223, 1970, 2149, 2185
MbiI CCGCTC 1 cut(s) 62
MfeI CAATTG 3 cut(s) 933, 1212, 1287
MhlI GDGCHC 1 cut(s) 552
MlyI GAGTC 3 cut(s) 231, 1034, 2182
MmeI TCCRAC 1 cut(s) 864
MroXI GAANNNNTTC 4 cut(s) 510, 1339, 1845, 2100
MseI TTAA 5 cut(s) 963, 986, 1494, 1730, 2231
MslI CAYNNNNRTG 6 cut(s) 421, 531, 1314, 1320, 1947, 1962
MspA1I CMGCKG 1 cut(s) 773
MspI CCGG 2 cut(s) 133, 1434
MspR9I CCNGG 5 cut(s) 134, 1435, 1586, 1933, 2242
MunI CAATTG 3 cut(s) 933, 1212, 1287
Mva1269I GAATGC 2 cut(s) 393, 526
MvaI CCWGG 3 cut(s) 1586, 1933, 2242
MwoI GCNNNNNNNGC 4 cut(s) 506, 1534, 1952, 2213
NciI CCSGG 2 cut(s) 134, 1435
NdeI CATATG 1 cut(s) 1677
NlaIV GGNNCC 6 cut(s) 417, 550, 662, 1156, 1583, 2246
NmeAIII GCCGAG 1 cut(s) 1175
NmuCI GTSAC 5 cut(s) 70, 77, 223, 1970, 2185
NspI RCATGY 4 cut(s) 932, 1482, 1510, 2230
NspV TTCGAA 2 cut(s) 1201, 2020
OliI CACNNNNGTG 1 cut(s) 421
PaeI GCATGC 1 cut(s) 932
PciI ACATGT 2 cut(s) 1506, 2226
PciSI GCTCTTC 1 cut(s) 2214
PctI GAATGC 2 cut(s) 393, 526
PdmI GAANNNNTTC 4 cut(s) 510, 1339, 1845, 2100
PfeI GAWTC 9 cut(s) 262, 694, 979, 1340, 1363, 1670, 1783, 1804, 2164
PflMI CCANNNNNTGG 1 cut(s) 2203
PkrI GCNGC 4 cut(s) 538, 772, 1428, 1954
Ple19I CGATCG 1 cut(s) 575
PleI GAGTC 3 cut(s) 230, 1033, 2182
PmaCI CACGTG 1 cut(s) 1254
PmlI CACGTG 1 cut(s) 1254
PpsI GAGTC 3 cut(s) 230, 1033, 2182
Ppu21I YACGTR 1 cut(s) 1254
PscI ACATGT 2 cut(s) 1506, 2226
PshAI GACNNNNGTC 1 cut(s) 1305
Psp6I CCWGG 3 cut(s) 1584, 1931, 2240
PspCI CACGTG 1 cut(s) 1254
PspGI CCWGG 3 cut(s) 1584, 1931, 2240
PspN4I GGNNCC 6 cut(s) 417, 550, 662, 1156, 1583, 2246
PspOMI GGGCCC 1 cut(s) 548
PspPI GGNCC 3 cut(s) 548, 549, 886
PstI CTGCAG 1 cut(s) 1428
PstNI CAGNNNCTG 1 cut(s) 536
PvuI CGATCG 1 cut(s) 575
RsaI GTAC 3 cut(s) 662, 1250, 1276
RsaNI GTAC 3 cut(s) 661, 1249, 1275
RseI CAYNNNNRTG 6 cut(s) 421, 531, 1314, 1320, 1947, 1962
SalI GTCGAC 1 cut(s) 1027
SapI GCTCTTC 1 cut(s) 2214
SaqAI TTAA 5 cut(s) 963, 986, 1494, 1730, 2231
SatI GCNGC 4 cut(s) 537, 771, 1427, 1953
Sau96I GGNCC 3 cut(s) 548, 549, 886
SchI GAGTC 3 cut(s) 231, 1034, 2182
ScrFI CCNGG 5 cut(s) 134, 1435, 1586, 1933, 2242
SduI GDGCHC 1 cut(s) 552
SfaNI GCATC 7 cut(s) 105, 508, 523, 1116, 1153, 1939, 2245
SfcI CTRYAG 2 cut(s) 1036, 1424
SfuI TTCGAA 2 cut(s) 1201, 2020
SinI GGWCC 1 cut(s) 886
SmiMI CAYNNNNRTG 6 cut(s) 421, 531, 1314, 1320, 1947, 1962
SmlI CTYRAG 3 cut(s) 21, 1576, 2088
SmoI CTYRAG 3 cut(s) 21, 1576, 2088
SphI GCATGC 1 cut(s) 932
SsiI CCGC 5 cut(s) 14, 62, 771, 1005, 2248
SspMI CTAG 6 cut(s) 711, 920, 1100, 1568, 1661, 2213
StyD4I CCNGG 5 cut(s) 132, 1433, 1584, 1931, 2240
TaaI ACNGT 8 cut(s) 423, 544, 780, 805, 1040, 1279, 2068, 2309
TaiI ACGT 4 cut(s) 171, 1256, 1668, 1972
TaqII GACCGA 1 cut(s) 58
TatI WGTACW 2 cut(s) 1248, 1274
TauI GCSGC 1 cut(s) 773
TfiI GAWTC 9 cut(s) 262, 694, 979, 1340, 1363, 1670, 1783, 1804, 2164
Tru1I TTAA 5 cut(s) 963, 986, 1494, 1730, 2231
Tru9I TTAA 5 cut(s) 963, 986, 1494, 1730, 2231
TscAI CASTG 5 cut(s) 428, 1117, 1122, 1473, 1638
TseFI GTSAC 5 cut(s) 70, 77, 223, 1970, 2185
TseI GCWGC 3 cut(s) 536, 1426, 1952
Tsp45I GTSAC 5 cut(s) 70, 77, 223, 1970, 2185
TspDTI ATGAA 9 cut(s) 340, 519, 849, 971, 1148, 1535, 1796, 1807, 1980
TspRI CASTG 5 cut(s) 428, 1117, 1122, 1473, 1638
Van91I CCANNNNNTGG 1 cut(s) 2203
VpaK11BI GGWCC 1 cut(s) 886
XapI RAATTY 3 cut(s) 291, 1455, 2220
XbaI TCTAGA 1 cut(s) 919
XceI RCATGY 4 cut(s) 932, 1482, 1510, 2230
XmiI GTMKAC 1 cut(s) 1028
XmnI GAANNNNTTC 4 cut(s) 510, 1339, 1845, 2100
XspI CTAG 6 cut(s) 711, 920, 1100, 1568, 1661, 2213
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.