MD00G1050100.v1.1

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0002668
Physical Location & Seq
Reverse (-)
9478919 .. 9486200
7282 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1050100.v1.1.491

Sequence Viewer

Length: 2064 bp
ATGCAAGGTCAAGGGAGTGGTATTGATACTTTCATTGAAATCACAGATGTTGGGCTTGGATCTCTCTCAAACAGCACTACTATGAGTCAGCAGACTTCTTTGAATAATATGCTAAATCCTGTTGAAAACCGGTTGTCAAGTTATATGGTGACATCTGGTGAGATTCCGTGTTTAAATGCCTCTAGTAACCAGAACTTGAGTGGTTCAGGTGAACCTAGCTCTAGATTGGGTCCAGTTGATGATGATGGAATGAAATTGGGACAAGGTTGGTCATCTCCTTATAGTGCTTGTCCTGTGATTGGTCATAGTTCAGAAGAAAGACGAGTTGAACAAAATATCCTTTTTCCTGGGAGAGCTCTTATTGGAATCAGTGGCAATCAGATTCAAGATGGGCCATCTTTTTCTCAGGGCTTTCAAAATGAAAATATAAATTCTGGATATGTGGGAAGTAGTGGTAATACTGTGCCTGGCATGGAAGATGATATTTCAGGTGGATTAGAAACAGAACAGACGTCTTCTGGTAGTGCTTCACCTGATATTGTTGGGACTTTATCCAGAAGCACCGATTTTCTGGTTGAGGAAAATGATGGGAACCCAGGTTCTTCTTTTGGATCTTGGGGTTTATCGTGCAAGCGAAAGGCCCTTGAGGGTACTTCAGGCCAGGCTTATTCTGGTGGAAGTTCTAGCTCCCTTCCACAAGCTGAAACTAGTGCATGGAATACTGTTTCTGCTCGTTATAGTCCTAGTAATTTAAGTTTATCTACACCGTTGCGTAGTTCTCCTAGTGGTGGTCCTTCAGAACAAAACTCAAGAACCAGGTTTGGTTTGATACTTGGGGCATCTGATACCTTTCCTTCAATAGATGCAACAGGAAATGGAGCAAGCTCACTGCAGGAATTTTTTTGTAGGGGGGCTTGTTCTGGGCCTCAGCAAGAATCTGCTCCATCCAACTTATCATCACAATGGAGGTCAACAACTGCAGCTGCAACAAATTCGGCTGGTCCCCAAAGCCAGTTTCCTGGAATGCATATGAGTGGTTTGTCAGGAAACATGCTTCCTTTTCCTTGGACTGGTGCTTCCAGTTCAAGAGTGGGCAGCTTACCAAGTTCTCTTTTGTCTGGATATAGAGGTGCTGAGTTAAGAGGAGAAGCAAACTTAAGAAGCATTCCCAGAAACAATGTGGAGCATTCAACTTTTGCAACTGCCAGTGATATGAGAAATTCAGCACAGGATCCCACAATTTGGGGTCTGGCCTCTGGAGAGTTAAGCATTTCTGTAGGTGCCCCTTCCTCTTCAAGAAGTAGCCCCAGTTCTGGCATTCGTTTTGTGCCTACTCCTGCATTGATCCGTGCCAACAACTTTCCATCTGAAAATCATCAAAGAACAATAGATTGGTCTTTGTTTCCTTCTATTGATTCTCAACCCGGAGGTCATAGTCATTTTAACTCAGTACCTTCAGGACCTGCTTCCTCACAGGATTCAGGAAGTTCTTCTGGGTCTAATAACCAGGGTCGTCACTTACCATATCCTAGGTCCGCATTCTTGGTGGACAGACAAGGTGATGATGCTCTTAATATGCCCCAGTCACTGCAGGCATTAGCAGCTGACATCGAAGGGAGGCGCCGACTTATATCTGAGATTCGTCAAGTTTTGAATGCTATGCGTAGGGGTGAGAACTTACGCGCCGAGGATTATATGCTCCTTGACCCATTCATATATCATGGCATGGCTGAGATGCACGACAGGCACAGAGACATGCGCCTTGATGTTGATAACATGTCTTATGAGGAACTGCTGGCACTGGGGGAACGTATAGGAGATGTTAGCACTGGACTGAGTGAGGAGACTATTATGAAGTGCATGAAACAGCGAATATTTCTCTGTATTTCAGAAAAATCCCCAGCAGATCTGGAACCATGCTGTGTCTGTCAGGAGGCTTATGCTGATGAGGATGATATTGGCACACTAGATTGTGGGCATCACTTCCACACCAGTTGCATCAAACAATGGCTAATGCAGAAGAATCTGTGCCCGATTTGTAAAACGACAGCCCTGCTTACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000209 GO:0000902 GO:0000904 GO:0003006 GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006355 GO:0006357 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0006928 GO:0006935 GO:0007275 GO:0007399 GO:0007409 GO:0007411 GO:0007568 GO:0008045 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009605 GO:0009653 GO:0009791 GO:0009889 GO:0009966 GO:0009967 GO:0009987 GO:0010228 GO:0010259 GO:0010468 GO:0010498 GO:0010556 GO:0010646 GO:0010647 GO:0016043 GO:0016567 GO:0016740 GO:0019219 GO:0019222 GO:0019538 GO:0019787 GO:0019941 GO:0021675 GO:0022008 GO:0022414 GO:0023051 GO:0023056 GO:0030030 GO:0030154 GO:0030163 GO:0030182 GO:0030510 GO:0030513 GO:0031175 GO:0031323 GO:0031326 GO:0032446 GO:0032501 GO:0032502 GO:0032989 GO:0032990 GO:0032991 GO:0035107 GO:0035108 GO:0035136 GO:0036211 GO:0040011 GO:0042221 GO:0042330 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044464 GO:0048468 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048608 GO:0048666 GO:0048667 GO:0048699 GO:0048731 GO:0048736 GO:0048812 GO:0048856 GO:0048858 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0051603 GO:0060173 GO:0060255 GO:0060384 GO:0061061 GO:0061458 GO:0061564 GO:0061630 GO:0061659 GO:0065007 GO:0070647 GO:0071704 GO:0071840 GO:0080090 GO:0090092 GO:0090100 GO:0090287 GO:0097485 GO:0120036 GO:0120039 GO:0140096 GO:1901564 GO:1901565 GO:1901575 GO:1903506 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

688

Amino Acids

73.12

Weight (kDa)

4.97

Isoelectric Point (pI)

61.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 638 - 681 2e-08 RING-H2 zinc finger domain
zf-RING_2 PF13639 640 - 681 1.6e-12 Ring finger domain
zf-C3HC4_2 PF13923 640 - 680 5.7e-07 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 640 - 680 3.6e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 515
Acc36I ACCTGC 1 cut(s) 1471
AccB1I GGYRCC 2 cut(s) 1280, 1620
AccB7I CCANNNNNTGG 1 cut(s) 1242
AccII CGCG 1 cut(s) 1683
AciI CCGC 1 cut(s) 1536
AclWI GGATC 5 cut(s) 67, 619, 1226, 1239, 1339
AcsI RAATTY 4 cut(s) 430, 896, 991, 1219
AcuI CTGAAG 3 cut(s) 639, 780, 1440
AcyI GRCGYC 2 cut(s) 512, 1621
AfaI GTAC 2 cut(s) 652, 1452
AfiI CCNNNNNNNGG 5 cut(s) 299, 788, 1070, 1242, 1313
AflII CTTAAG 1 cut(s) 1156
AflIII ACRYGT 1 cut(s) 1776
AgeI ACCGGT 1 cut(s) 129
AhlI ACTAGT 1 cut(s) 707
AjnI CCWGG 7 cut(s) 346, 466, 595, 660, 815, 1018, 1506
AjuI GAANNNNNNNTTGG 4 cut(s) 999, 1031, 1375, 1407
AloI GAACNNNNNNTCC 4 cut(s) 321, 353, 583, 615
AluBI AGCT 8 cut(s) 219, 356, 687, 701, 885, 983, 1098, 1604
AluI AGCT 8 cut(s) 219, 356, 687, 701, 885, 983, 1098, 1604
Alw21I GWGCWC 1 cut(s) 358
Alw26I GTCTC 2 cut(s) 1746, 1838
AlwI GGATC 5 cut(s) 67, 619, 1226, 1239, 1339
AlwNI CAGNNNCTG 2 cut(s) 1463, 1588
AoxI GGCC 5 cut(s) 392, 639, 658, 923, 1251
ApeKI GCWGC 4 cut(s) 980, 983, 1095, 1601
ApoI RAATTY 4 cut(s) 430, 896, 991, 1219
AsiGI ACCGGT 1 cut(s) 129
Asp700I GAANNNNTTC 1 cut(s) 1489
AspA2I CCTAGG 1 cut(s) 1529
AspLEI GCGC 3 cut(s) 1623, 1685, 1761
AspS9I GGNCC 8 cut(s) 230, 392, 640, 791, 923, 1001, 1460, 1533
AsuC2I CCSGG 1 cut(s) 1425
AsuHPI GGTGA 6 cut(s) 160, 170, 221, 522, 1571, 1682
AvaII GGWCC 5 cut(s) 230, 791, 1001, 1460, 1533
AvrII CCTAGG 1 cut(s) 1529
BaeGI GKGCMC 2 cut(s) 1285, 2033
BamHI GGATCC 1 cut(s) 1231
BanI GGYRCC 2 cut(s) 1280, 1620
BanII GRGCYC 1 cut(s) 358
BbsI GAAGAC 1 cut(s) 507
Bbv12I GWGCWC 1 cut(s) 358
BbvCI CCTCAGC 1 cut(s) 927
BbvI GCAGC 4 cut(s) 970, 992, 1107, 1613
BccI CCATC 6 cut(s) 239, 383, 403, 581, 952, 1372
BcgI CGANNNNNNTGC 3 cut(s) 975, 1009, 2035
BciT130I CCWGG 7 cut(s) 348, 468, 597, 662, 817, 1020, 1508
BcnI CCSGG 1 cut(s) 1425
BcoDI GTCTC 2 cut(s) 1746, 1838
BcuI ACTAGT 1 cut(s) 707
BfaI CTAG 9 cut(s) 183, 216, 222, 684, 708, 744, 783, 1530, 1967
BfmI CTRYAG 4 cut(s) 890, 978, 1275, 1589
BfoI RGCGCY 1 cut(s) 1624
BfrI CTTAAG 1 cut(s) 1156
BfuAI ACCTGC 1 cut(s) 1471
BglII AGATCT 1 cut(s) 1906
BisI GCNGC 4 cut(s) 981, 984, 1096, 1602
BlnI CCTAGG 1 cut(s) 1529
BlsI GCNGC 4 cut(s) 982, 985, 1097, 1603
Bme1390I CCNGG 8 cut(s) 348, 468, 597, 662, 817, 1020, 1425, 1508
Bme18I GGWCC 5 cut(s) 230, 791, 1001, 1460, 1533
BmgT120I GGNCC 8 cut(s) 230, 392, 640, 791, 923, 1001, 1460, 1533
BmiI GGNNCC 7 cut(s) 231, 593, 1003, 1233, 1282, 1622, 1914
BmrFI CCNGG 8 cut(s) 348, 468, 597, 662, 817, 1020, 1425, 1508
BmrI ACTGGG 3 cut(s) 1302, 1576, 1811
BmsI GCATC 6 cut(s) 848, 853, 1555, 1725, 1987, 2007
BmuI ACTGGG 3 cut(s) 1302, 1576, 1811
BpiI GAAGAC 1 cut(s) 507
BpmI CTGGAG 1 cut(s) 1278
Bpu10I CCTNAGC 1 cut(s) 927
BpuEI CTTGAG 3 cut(s) 217, 665, 793
BpuMI CCSGG 1 cut(s) 1425
BsaHI GRCGYC 2 cut(s) 512, 1621
BsaJI CCNNGG 6 cut(s) 347, 595, 1064, 1507, 1529, 1686
BsaWI WCCGGW 1 cut(s) 129
Bsc4I CCNNNNNNNGG 5 cut(s) 299, 788, 1070, 1242, 1313
Bse118I RCCGGY 1 cut(s) 129
BseBI CCWGG 7 cut(s) 348, 468, 597, 662, 817, 1020, 1508
BseDI CCNNGG 6 cut(s) 347, 595, 1064, 1507, 1529, 1686
BseGI GGATG 2 cut(s) 944, 1957
BseLI CCNNNNNNNGG 5 cut(s) 299, 788, 1070, 1242, 1313
BseMII CTCAG 7 cut(s) 419, 941, 1125, 1461, 1626, 1722, 1826
BseRI GAGGAG 2 cut(s) 1158, 1856
BseSI GKGCMC 2 cut(s) 1285, 2033
BseXI GCAGC 4 cut(s) 970, 992, 1107, 1613
BseYI CCCAGC 1 cut(s) 1900
Bsh1236I CGCG 1 cut(s) 1683
BshFI GGCC 5 cut(s) 394, 641, 660, 925, 1253
BshNI GGYRCC 2 cut(s) 1280, 1620
BshTI ACCGGT 1 cut(s) 129
BsiHKAI GWGCWC 1 cut(s) 358
BsiSI CCGG 2 cut(s) 130, 1425
BslFI GGGAC 3 cut(s) 273, 559, 987
BslI CCNNNNNNNGG 5 cut(s) 299, 788, 1070, 1242, 1313
BsmAI GTCTC 2 cut(s) 1746, 1838
BsmFI GGGAC 3 cut(s) 273, 559, 987
BsmI GAATGC 6 cut(s) 1029, 1164, 1186, 1317, 1538, 1660
BsnI GGCC 5 cut(s) 394, 641, 660, 925, 1253
Bsp1286I GDGCHC 3 cut(s) 358, 1285, 2033
Bsp143I GATC 5 cut(s) 59, 611, 1231, 1344, 1906
BspACI CCGC 1 cut(s) 1536
BspANI GGCC 5 cut(s) 394, 641, 660, 925, 1253
BspCNI CTCAG 7 cut(s) 418, 940, 1126, 1460, 1627, 1723, 1827
BspFNI CGCG 1 cut(s) 1683
BspLI GGNNCC 7 cut(s) 231, 593, 1003, 1233, 1282, 1622, 1914
BspMAI CTGCAG 3 cut(s) 894, 982, 1593
BspMI ACCTGC 1 cut(s) 1471
BspPI GGATC 5 cut(s) 67, 619, 1226, 1239, 1339
BspT107I GGYRCC 2 cut(s) 1280, 1620
BspTI CTTAAG 1 cut(s) 1156
BsrFI RCCGGY 1 cut(s) 129
BssAI RCCGGY 1 cut(s) 129
BssECI CCNNGG 6 cut(s) 347, 595, 1064, 1507, 1529, 1686
BssMI GATC 5 cut(s) 59, 611, 1231, 1344, 1906
BssNI GRCGYC 2 cut(s) 512, 1621
BssT1I CCWWGG 2 cut(s) 1064, 1529
Bst2UI CCWGG 7 cut(s) 348, 468, 597, 662, 817, 1020, 1508
Bst4CI ACNGT 3 cut(s) 463, 724, 768
Bst6I CTCTTC 1 cut(s) 1297
BstACI GRCGYC 2 cut(s) 512, 1621
BstAFI CTTAAG 1 cut(s) 1156
BstC8I GCNNGC 4 cut(s) 632, 883, 1593, 1797
BstDEI CTNAG 7 cut(s) 405, 927, 1134, 1447, 1635, 1731, 1835
BstF5I GGATG 2 cut(s) 944, 1957
BstFNI CGCG 1 cut(s) 1683
BstH2I RGCGCY 1 cut(s) 1624
BstHHI GCGC 3 cut(s) 1623, 1685, 1761
BstKTI GATC 5 cut(s) 62, 614, 1234, 1347, 1909
BstMAI GTCTC 2 cut(s) 1746, 1838
BstMBI GATC 5 cut(s) 59, 611, 1231, 1344, 1906
BstMWI GCNNNNNNNGC 2 cut(s) 1601, 1744
BstNI CCWGG 7 cut(s) 348, 468, 597, 662, 817, 1020, 1508
BstNSI RCATGY 3 cut(s) 1054, 1759, 1780
BstSCI CCNGG 8 cut(s) 346, 466, 595, 660, 815, 1018, 1423, 1506
BstSFI CTRYAG 4 cut(s) 890, 978, 1275, 1589
BstSLI GKGCMC 2 cut(s) 1285, 2033
BstUI CGCG 1 cut(s) 1683
BstV1I GCAGC 4 cut(s) 970, 992, 1107, 1613
BstV2I GAAGAC 1 cut(s) 507
BstX2I RGATCY 4 cut(s) 59, 611, 1231, 1906
BstXI CCANNNNNNTGG 1 cut(s) 1019
BstYI RGATCY 4 cut(s) 59, 611, 1231, 1906
BsuRI GGCC 5 cut(s) 394, 641, 660, 925, 1253
BtsCI GGATG 2 cut(s) 944, 1957
BtsI GCAGTG 2 cut(s) 887, 1586
BtsIMutI CAGTG 6 cut(s) 376, 887, 1213, 1586, 1799, 1827
BveI ACCTGC 1 cut(s) 1471
Cac8I GCNNGC 4 cut(s) 632, 883, 1593, 1797
CaiI CAGNNNCTG 2 cut(s) 1463, 1588
CfoI GCGC 3 cut(s) 1623, 1685, 1761
Cfr10I RCCGGY 1 cut(s) 129
Cfr13I GGNCC 8 cut(s) 230, 392, 640, 791, 923, 1001, 1460, 1533
CsiI ACCWGGT 1 cut(s) 815
Csp6I GTAC 2 cut(s) 651, 1451
CspAI ACCGGT 1 cut(s) 129
CspCI CAANNNNNGTGG 2 cut(s) 1976, 2011
CviAII CATG 9 cut(s) 472, 714, 1051, 1721, 1726, 1756, 1777, 1861, 1917
CviQI GTAC 2 cut(s) 651, 1451
DdeI CTNAG 7 cut(s) 405, 927, 1134, 1447, 1635, 1731, 1835
DinI GGCGCC 1 cut(s) 1622
DpnI GATC 5 cut(s) 61, 613, 1233, 1346, 1908
DpnII GATC 5 cut(s) 59, 611, 1231, 1344, 1906
DraI TTTAAA 1 cut(s) 174
Eam1104I CTCTTC 1 cut(s) 1297
EarI CTCTTC 1 cut(s) 1297
Ecl136II GAGCTC 1 cut(s) 356
Eco130I CCWWGG 2 cut(s) 1064, 1529
Eco24I GRGCYC 1 cut(s) 358
Eco47I GGWCC 5 cut(s) 230, 791, 1001, 1460, 1533
Eco53kI GAGCTC 1 cut(s) 356
Eco57I CTGAAG 3 cut(s) 639, 780, 1440
EcoICRI GAGCTC 1 cut(s) 356
EcoO109I RGGNCCY 2 cut(s) 640, 1460
EcoRII CCWGG 7 cut(s) 346, 466, 595, 660, 815, 1018, 1506
EcoT14I CCWWGG 2 cut(s) 1064, 1529
EcoT22I ATGCAT 1 cut(s) 1029
EcoT38I GRGCYC 1 cut(s) 358
EgeI GGCGCC 1 cut(s) 1622
EheI GGCGCC 1 cut(s) 1622
ErhI CCWWGG 2 cut(s) 1064, 1529
FaeI CATG 9 cut(s) 475, 717, 1054, 1724, 1729, 1759, 1780, 1864, 1920
FaqI GGGAC 3 cut(s) 273, 559, 987
FatI CATG 9 cut(s) 471, 713, 1050, 1720, 1725, 1755, 1776, 1860, 1916
FauNDI CATATG 1 cut(s) 1029
Fnu4HI GCNGC 4 cut(s) 981, 984, 1096, 1602
FokI GGATG 2 cut(s) 931, 1964
FriOI GRGCYC 1 cut(s) 358
Fsp4HI GCNGC 4 cut(s) 981, 984, 1096, 1602
FspBI CTAG 9 cut(s) 183, 216, 222, 684, 708, 744, 783, 1530, 1967
GlaI GCGC 3 cut(s) 1622, 1684, 1760
GluI GCNGC 4 cut(s) 981, 984, 1096, 1602
GsaI CCCAGC 1 cut(s) 1904
GsuI CTGGAG 1 cut(s) 1278
HaeII RGCGCY 1 cut(s) 1624
HaeIII GGCC 5 cut(s) 394, 641, 660, 925, 1253
HapII CCGG 2 cut(s) 130, 1425
HhaI GCGC 3 cut(s) 1623, 1685, 1761
Hin1I GRCGYC 2 cut(s) 512, 1621
Hin1II CATG 9 cut(s) 475, 717, 1054, 1724, 1729, 1759, 1780, 1864, 1920
Hin6I GCGC 3 cut(s) 1621, 1683, 1759
HinP1I GCGC 3 cut(s) 1621, 1683, 1759
HincII GTYRAC 1 cut(s) 972
HindII GTYRAC 1 cut(s) 972
HinfI GANTC 9 cut(s) 85, 163, 366, 382, 935, 1415, 1478, 1639, 2023
HpaII CCGG 2 cut(s) 130, 1425
HphI GGTGA 6 cut(s) 160, 170, 221, 522, 1571, 1682
Hpy166II GTNNAC 3 cut(s) 212, 972, 1549
Hpy188I TCNGA 7 cut(s) 313, 381, 799, 844, 1369, 1636, 1891
Hpy8I GTNNAC 3 cut(s) 212, 972, 1549
HpyAV CCTTC 7 cut(s) 701, 804, 864, 1296, 1416, 1464, 1607
HpyCH4III ACNGT 3 cut(s) 463, 724, 768
HpyCH4IV ACGT 2 cut(s) 512, 1810
HpyF10VI GCNNNNNNNGC 2 cut(s) 1601, 1744
HpyF3I CTNAG 7 cut(s) 405, 927, 1134, 1447, 1635, 1731, 1835
HpySE526I ACGT 2 cut(s) 512, 1810
Hsp92I GRCGYC 2 cut(s) 512, 1621
Hsp92II CATG 9 cut(s) 475, 717, 1054, 1724, 1729, 1759, 1780, 1864, 1920
HspAI GCGC 3 cut(s) 1621, 1683, 1759
KasI GGCGCC 1 cut(s) 1620
Kzo9I GATC 5 cut(s) 59, 611, 1231, 1344, 1906
LmnI GCTCC 5 cut(s) 692, 878, 946, 1183, 1704
Lsp1109I GCAGC 4 cut(s) 970, 992, 1107, 1613
LweI GCATC 6 cut(s) 848, 853, 1555, 1725, 1987, 2007
MabI ACCWGGT 1 cut(s) 815
MaeI CTAG 9 cut(s) 183, 216, 222, 684, 708, 744, 783, 1530, 1967
MaeII ACGT 2 cut(s) 512, 1810
MaeIII GTNAC 4 cut(s) 148, 185, 1514, 1584
MalI GATC 5 cut(s) 61, 613, 1233, 1346, 1908
MboI GATC 5 cut(s) 59, 611, 1231, 1344, 1906
MboII GAAGA 7 cut(s) 326, 488, 507, 594, 1284, 1482, 2032
MflI RGATCY 4 cut(s) 59, 611, 1231, 1906
MhlI GDGCHC 3 cut(s) 358, 1285, 2033
MluCI AATT 7 cut(s) 254, 430, 748, 896, 991, 1219, 1239
Mly113I GGCGCC 1 cut(s) 1621
MlyI GAGTC 1 cut(s) 94
MmeI TCCRAC 1 cut(s) 972
Mph1103I ATGCAT 1 cut(s) 1029
MroXI GAANNNNTTC 1 cut(s) 1489
MseI TTAA 7 cut(s) 173, 752, 1139, 1157, 1265, 1443, 1572
MslI CAYNNNNRTG 3 cut(s) 80, 961, 1032
MspA1I CMGCKG 2 cut(s) 983, 1604
MspCI CTTAAG 1 cut(s) 1156
MspI CCGG 2 cut(s) 130, 1425
MspR9I CCNGG 8 cut(s) 348, 468, 597, 662, 817, 1020, 1425, 1508
Mva1269I GAATGC 6 cut(s) 1029, 1164, 1186, 1317, 1538, 1660
MvaI CCWGG 7 cut(s) 348, 468, 597, 662, 817, 1020, 1508
MvnI CGCG 1 cut(s) 1683
MwoI GCNNNNNNNGC 2 cut(s) 1601, 1744
NarI GGCGCC 1 cut(s) 1621
NciI CCSGG 1 cut(s) 1425
NdeI CATATG 1 cut(s) 1029
NdeII GATC 5 cut(s) 59, 611, 1231, 1344, 1906
NlaIII CATG 9 cut(s) 475, 717, 1054, 1724, 1729, 1759, 1780, 1864, 1920
NlaIV GGNNCC 7 cut(s) 231, 593, 1003, 1233, 1282, 1622, 1914
NmeAIII GCCGAG 1 cut(s) 1711
NmuCI GTSAC 3 cut(s) 148, 1514, 1584
NsiI ATGCAT 1 cut(s) 1029
NspI RCATGY 3 cut(s) 1054, 1759, 1780
PciI ACATGT 1 cut(s) 1776
PctI GAATGC 6 cut(s) 1029, 1164, 1186, 1317, 1538, 1660
PdmI GAANNNNTTC 1 cut(s) 1489
PfeI GAWTC 8 cut(s) 163, 366, 382, 935, 1415, 1478, 1639, 2023
PflMI CCANNNNNTGG 1 cut(s) 1242
PfoI TCCNGGA 1 cut(s) 1018
PinAI ACCGGT 1 cut(s) 129
PkrI GCNGC 4 cut(s) 982, 985, 1097, 1603
PleI GAGTC 1 cut(s) 93
PluTI GGCGCC 1 cut(s) 1624
PpsI GAGTC 1 cut(s) 93
PpuMI RGGWCCY 1 cut(s) 1460
PscI ACATGT 1 cut(s) 1776
Psp124BI GAGCTC 1 cut(s) 358
Psp5II RGGWCCY 1 cut(s) 1460
Psp6I CCWGG 7 cut(s) 346, 466, 595, 660, 815, 1018, 1506
PspFI CCCAGC 1 cut(s) 1900
PspGI CCWGG 7 cut(s) 346, 466, 595, 660, 815, 1018, 1506
PspN4I GGNNCC 7 cut(s) 231, 593, 1003, 1233, 1282, 1622, 1914
PspPI GGNCC 8 cut(s) 230, 392, 640, 791, 923, 1001, 1460, 1533
PspPPI RGGWCCY 1 cut(s) 1460
PsrI GAACNNNNNNTAC 2 cut(s) 1294, 1326
PstI CTGCAG 3 cut(s) 894, 982, 1593
PstNI CAGNNNCTG 2 cut(s) 1463, 1588
PsuI RGATCY 4 cut(s) 59, 611, 1231, 1906
PvuII CAGCTG 2 cut(s) 983, 1604
RsaI GTAC 2 cut(s) 652, 1452
RsaNI GTAC 2 cut(s) 651, 1451
RseI CAYNNNNRTG 3 cut(s) 80, 961, 1032
SacI GAGCTC 1 cut(s) 358
SaqAI TTAA 7 cut(s) 173, 752, 1139, 1157, 1265, 1443, 1572
SatI GCNGC 4 cut(s) 981, 984, 1096, 1602
Sau3AI GATC 5 cut(s) 59, 611, 1231, 1344, 1906
Sau96I GGNCC 8 cut(s) 230, 392, 640, 791, 923, 1001, 1460, 1533
SchI GAGTC 1 cut(s) 94
ScrFI CCNGG 8 cut(s) 348, 468, 597, 662, 817, 1020, 1425, 1508
SduI GDGCHC 3 cut(s) 358, 1285, 2033
SexAI ACCWGGT 1 cut(s) 815
SfaNI GCATC 6 cut(s) 848, 853, 1555, 1725, 1987, 2007
SfcI CTRYAG 4 cut(s) 890, 978, 1275, 1589
SfoI GGCGCC 1 cut(s) 1622
SinI GGWCC 5 cut(s) 230, 791, 1001, 1460, 1533
SmiMI CAYNNNNRTG 3 cut(s) 80, 961, 1032
SmlI CTYRAG 4 cut(s) 196, 644, 808, 1156
SmoI CTYRAG 4 cut(s) 196, 644, 808, 1156
SpeI ACTAGT 1 cut(s) 707
Sse9I AATT 7 cut(s) 254, 430, 748, 896, 991, 1219, 1239
SsiI CCGC 1 cut(s) 1536
SspDI GGCGCC 1 cut(s) 1620
SspI AATATT 1 cut(s) 1875
SspMI CTAG 9 cut(s) 183, 216, 222, 684, 708, 744, 783, 1530, 1967
SstI GAGCTC 1 cut(s) 358
StyD4I CCNGG 8 cut(s) 346, 466, 595, 660, 815, 1018, 1423, 1506
StyI CCWWGG 2 cut(s) 1064, 1529
TaaI ACNGT 3 cut(s) 463, 724, 768
TaiI ACGT 2 cut(s) 515, 1813
TaqI TCGA 1 cut(s) 1611
TasI AATT 7 cut(s) 254, 430, 748, 896, 991, 1219, 1239
TfiI GAWTC 8 cut(s) 163, 366, 382, 935, 1415, 1478, 1639, 2023
Tru1I TTAA 7 cut(s) 173, 752, 1139, 1157, 1265, 1443, 1572
Tru9I TTAA 7 cut(s) 173, 752, 1139, 1157, 1265, 1443, 1572
TscAI CASTG 6 cut(s) 376, 894, 1213, 1593, 1806, 1834
TseFI GTSAC 3 cut(s) 148, 1514, 1584
TseI GCWGC 4 cut(s) 980, 983, 1095, 1601
Tsp45I GTSAC 3 cut(s) 148, 1514, 1584
TspDTI ATGAA 6 cut(s) 22, 266, 435, 1702, 1868, 1877
TspGWI ACGGA 2 cut(s) 156, 1337
TspRI CASTG 6 cut(s) 376, 894, 1213, 1593, 1806, 1834
Van91I CCANNNNNTGG 1 cut(s) 1242
Vha464I CTTAAG 1 cut(s) 1156
VpaK11BI GGWCC 5 cut(s) 230, 791, 1001, 1460, 1533
XapI RAATTY 4 cut(s) 430, 896, 991, 1219
XbaI TCTAGA 1 cut(s) 221
XceI RCATGY 3 cut(s) 1054, 1759, 1780
XcmI CCANNNNNNNNNTGG 4 cut(s) 197, 668, 1087, 1177
XmaJI CCTAGG 1 cut(s) 1529
XmnI GAANNNNTTC 1 cut(s) 1489
XspI CTAG 9 cut(s) 183, 216, 222, 684, 708, 744, 783, 1530, 1967
ZraI GACGTC 1 cut(s) 513
Zsp2I ATGCAT 1 cut(s) 1029
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.