Rorug05G0566200

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0002668
Physical Location & Seq
Reverse (-)
75951774 .. 75952807
1034 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0566200.1

Sequence Viewer

Length: 861 bp
ATGGGACTTCAAGCCGGTTGGACCCTGCCGGGGCTCACCCCCAAGCCCTGCGACACCTGCAAGTCGTCGCCGTCGGCCGTCTTCTGCCGCGCCGACTCGGCCTACCTCTGCCTGACCTGCGACTCCAAGATCCACTGCGCCAACAAGCTCGCGTCGCGCCACCAGCGCGTGTGGATGTGCGAGGTCTGCGAGCAAGCTCCGGCCGCCGTCACGTGCAATGCCGACGCCGCCGCCCTCTGCGTCACGTGCGACGCCGACATCCACTCCGCCAATCCGCTCGCGCGTCGCCACGAGCGCGTCCCCGTGGAGCCGTTCCTAGACTCGGCCGAGTCCATCGTCAAATCCACCTCGGCTGCTGGCCTCAACGACGCCGTTTCGCCTCTTAAAAACGAAGACGCCGATTCTCTGCTCATTCCCAACCTGAATTTCAGCGCCAAGTTCATTGATGCTCTCGACATCAAGCCCGGGGATATGTTCTTCCCGGAAATGGAATCGTTATTCGAACTCGATTACCCGAACCCGATCCAAAACTCCAGTTCGGGTATGGATAGCGTGGTTCCGGTTCAACCCGACCCGATTCCACCTCCTCCTGTCGTCAACCAACCCCCTCATGAAAATTGCTTCGACATTGACTTCTGCAGAAACAAGCTCTCTTCCTCCTTCAGTTATCCAGCTCAATCAATCAGCCAAAGCGTCTCGTCGTCCTCCTTAGAGGTGGGAGTTGTTCCCGACGGAAATTCATTGTCCGAGAAAGAGAGGGGGCTAATGTATGAGACGGGGACCAGCTGGGGGGGACCAGGGGAGCGAATTGACGGCGTCATTGACGTCGTGTATGTATATTGGGTCGGGTTTCATATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000209 GO:0000902 GO:0000904 GO:0003006 GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006355 GO:0006357 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0006928 GO:0006935 GO:0007275 GO:0007399 GO:0007409 GO:0007411 GO:0007568 GO:0008045 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009605 GO:0009653 GO:0009791 GO:0009889 GO:0009966 GO:0009967 GO:0009987 GO:0010228 GO:0010259 GO:0010468 GO:0010498 GO:0010556 GO:0010646 GO:0010647 GO:0016043 GO:0016567 GO:0016740 GO:0019219 GO:0019222 GO:0019538 GO:0019787 GO:0019941 GO:0021675 GO:0022008 GO:0022414 GO:0023051 GO:0023056 GO:0030030 GO:0030154 GO:0030163 GO:0030182 GO:0030510 GO:0030513 GO:0031175 GO:0031323 GO:0031326 GO:0032446 GO:0032501 GO:0032502 GO:0032989 GO:0032990 GO:0032991 GO:0035107 GO:0035108 GO:0035136 GO:0036211 GO:0040011 GO:0042221 GO:0042330 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044464 GO:0048468 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048608 GO:0048666 GO:0048667 GO:0048699 GO:0048731 GO:0048736 GO:0048812 GO:0048856 GO:0048858 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0051603 GO:0060173 GO:0060255 GO:0060384 GO:0061061 GO:0061458 GO:0061564 GO:0061630 GO:0061659 GO:0065007 GO:0070647 GO:0071704 GO:0071840 GO:0080090 GO:0090092 GO:0090100 GO:0090287 GO:0097485 GO:0120036 GO:0120039 GO:0140096 GO:1901564 GO:1901565 GO:1901575 GO:1903506 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

286

Amino Acids

30.86

Weight (kDa)

4.58

Isoelectric Point (pI)

47.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-B_box PF00643 56 - 102 4.9e-07 B-box zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 65
AatII GACGTC 1 cut(s) 828
Acc36I ACCTGC 2 cut(s) 65, 125
AccBSI CCGCTC 1 cut(s) 277
AccII CGCG 7 cut(s) 90, 152, 157, 168, 281, 283, 297
AciI CCGC 6 cut(s) 88, 204, 228, 231, 267, 275
AclWI GGATC 2 cut(s) 124, 517
AcoI YGGCCR 3 cut(s) 75, 201, 324
AcsI RAATTY 2 cut(s) 424, 736
AcuI CTGAAG 1 cut(s) 646
AcvI CACGTG 2 cut(s) 213, 246
AcyI GRCGYC 6 cut(s) 225, 252, 369, 396, 816, 825
AfiI CCNNNNNNNGG 4 cut(s) 30, 322, 487, 789
AgsI TTSAA 2 cut(s) 11, 566
AjnI CCWGG 1 cut(s) 796
AjuI GAANNNNNNNTTGG 2 cut(s) 410, 442
AluBI AGCT 5 cut(s) 148, 197, 649, 674, 786
AluI AGCT 5 cut(s) 148, 197, 649, 674, 786
Alw26I GTCTC 2 cut(s) 700, 767
AlwI GGATC 2 cut(s) 124, 517
Ama87I CYCGRG 1 cut(s) 464
AoxI GGCC 5 cut(s) 75, 99, 201, 324, 358
ApeKI GCWGC 1 cut(s) 353
ApoI RAATTY 2 cut(s) 424, 736
AspLEI GCGC 7 cut(s) 92, 140, 159, 168, 283, 297, 434
AspS9I GGNCC 3 cut(s) 21, 780, 794
AsuC2I CCSGG 4 cut(s) 30, 465, 466, 482
AsuHPI GGTGA 1 cut(s) 28
AsuII TTCGAA 1 cut(s) 501
AvaI CYCGRG 1 cut(s) 464
AvaII GGWCC 3 cut(s) 21, 780, 794
BanII GRGCYC 1 cut(s) 36
BauI CACGAG 1 cut(s) 290
BbrPI CACGTG 2 cut(s) 213, 246
BbsI GAAGAC 2 cut(s) 73, 399
BbvI GCAGC 1 cut(s) 340
BccI CCATC 1 cut(s) 341
BceAI ACGGC 6 cut(s) 55, 62, 191, 295, 356, 829
BciT130I CCWGG 1 cut(s) 798
BcnI CCSGG 4 cut(s) 30, 465, 466, 482
BcoDI GTCTC 2 cut(s) 700, 767
BfaI CTAG 1 cut(s) 317
BfmI CTRYAG 1 cut(s) 637
BfoI RGCGCY 1 cut(s) 435
BfuAI ACCTGC 2 cut(s) 65, 125
BglI GCCNNNNNGGC 1 cut(s) 98
BisI GCNGC 5 cut(s) 88, 204, 228, 231, 354
BlsI GCNGC 5 cut(s) 89, 205, 229, 232, 355
Bme1390I CCNGG 5 cut(s) 30, 465, 466, 482, 798
Bme18I GGWCC 3 cut(s) 21, 780, 794
BmeT110I CYCGRG 1 cut(s) 464
BmgT120I GGNCC 3 cut(s) 21, 780, 794
BmiI GGNNCC 5 cut(s) 23, 309, 558, 781, 795
BmrFI CCNGG 5 cut(s) 30, 465, 466, 482, 798
BmsI GCATC 1 cut(s) 436
BpiI GAAGAC 2 cut(s) 73, 399
BpmI CTGGAG 1 cut(s) 517
Bpu14I TTCGAA 1 cut(s) 501
BpuMI CCSGG 4 cut(s) 30, 465, 466, 482
BsaAI YACGTR 2 cut(s) 213, 246
BsaHI GRCGYC 6 cut(s) 225, 252, 369, 396, 816, 825
BsaJI CCNNGG 6 cut(s) 29, 303, 348, 464, 465, 797
BsaWI WCCGGW 1 cut(s) 559
BsaXI ACNNNNNCTCC 2 cut(s) 248, 278
Bsc4I CCNNNNNNNGG 4 cut(s) 30, 322, 487, 789
Bse118I RCCGGY 1 cut(s) 14
Bse1I ACTGG 1 cut(s) 534
Bse3DI GCAATG 1 cut(s) 223
BseBI CCWGG 1 cut(s) 798
BseDI CCNNGG 6 cut(s) 29, 303, 348, 464, 465, 797
BseGI GGATG 2 cut(s) 180, 258
BseLI CCNNNNNNNGG 4 cut(s) 30, 322, 487, 789
BseMI GCAATG 1 cut(s) 223
BseNI ACTGG 1 cut(s) 534
BseRI GAGGAG 1 cut(s) 576
BseX3I CGGCCG 3 cut(s) 75, 201, 324
BseXI GCAGC 1 cut(s) 340
BseYI CCCAGC 1 cut(s) 786
Bsh1236I CGCG 7 cut(s) 90, 152, 157, 168, 281, 283, 297
Bsh1285I CGRYCG 3 cut(s) 78, 204, 327
BshFI GGCC 5 cut(s) 77, 101, 203, 326, 360
BsiEI CGRYCG 3 cut(s) 78, 204, 327
BsiHKCI CYCGRG 1 cut(s) 464
BsiSI CCGG 6 cut(s) 15, 29, 200, 465, 482, 560
BslFI GGGAC 4 cut(s) 18, 284, 793, 807
BslI CCNNNNNNNGG 4 cut(s) 30, 322, 487, 789
BsmAI GTCTC 2 cut(s) 700, 767
BsmBI CGTCTC 2 cut(s) 700, 767
BsmFI GGGAC 4 cut(s) 18, 284, 793, 807
BsnI GGCC 5 cut(s) 77, 101, 203, 326, 360
BsoBI CYCGRG 1 cut(s) 464
Bsp119I TTCGAA 1 cut(s) 501
Bsp1286I GDGCHC 1 cut(s) 36
Bsp143I GATC 2 cut(s) 129, 522
BspACI CCGC 6 cut(s) 88, 204, 228, 231, 267, 275
BspANI GGCC 5 cut(s) 77, 101, 203, 326, 360
BspFNI CGCG 7 cut(s) 90, 152, 157, 168, 281, 283, 297
BspHI TCATGA 1 cut(s) 610
BspLI GGNNCC 5 cut(s) 23, 309, 558, 781, 795
BspMAI CTGCAG 1 cut(s) 641
BspMI ACCTGC 2 cut(s) 65, 125
BspPI GGATC 2 cut(s) 124, 517
BspT104I TTCGAA 1 cut(s) 501
BsrBI CCGCTC 1 cut(s) 277
BsrDI GCAATG 1 cut(s) 223
BsrFI RCCGGY 1 cut(s) 14
BsrI ACTGG 1 cut(s) 534
BssAI RCCGGY 1 cut(s) 14
BssECI CCNNGG 6 cut(s) 29, 303, 348, 464, 465, 797
BssMI GATC 2 cut(s) 129, 522
BssNI GRCGYC 6 cut(s) 225, 252, 369, 396, 816, 825
BssSI CACGAG 1 cut(s) 290
Bst2BI CACGAG 1 cut(s) 290
Bst2UI CCWGG 1 cut(s) 798
Bst6I CTCTTC 1 cut(s) 658
BstACI GRCGYC 6 cut(s) 225, 252, 369, 396, 816, 825
BstBAI YACGTR 2 cut(s) 213, 246
BstBI TTCGAA 1 cut(s) 501
BstC8I GCNNGC 5 cut(s) 150, 191, 195, 279, 358
BstDEI CTNAG 1 cut(s) 709
BstDSI CCRYGG 1 cut(s) 303
BstF5I GGATG 2 cut(s) 180, 258
BstFNI CGCG 7 cut(s) 90, 152, 157, 168, 281, 283, 297
BstH2I RGCGCY 1 cut(s) 435
BstHHI GCGC 7 cut(s) 92, 140, 159, 168, 283, 297, 434
BstKTI GATC 2 cut(s) 132, 525
BstMAI GTCTC 2 cut(s) 700, 767
BstMBI GATC 2 cut(s) 129, 522
BstMCI CGRYCG 3 cut(s) 78, 204, 327
BstNI CCWGG 1 cut(s) 798
BstSCI CCNGG 5 cut(s) 28, 463, 464, 480, 796
BstSFI CTRYAG 1 cut(s) 637
BstUI CGCG 7 cut(s) 90, 152, 157, 168, 281, 283, 297
BstV1I GCAGC 1 cut(s) 340
BstV2I GAAGAC 2 cut(s) 73, 399
BstX2I RGATCY 1 cut(s) 129
BstYI RGATCY 1 cut(s) 129
BstZI CGGCCG 3 cut(s) 75, 201, 324
BsuRI GGCC 5 cut(s) 77, 101, 203, 326, 360
BtgI CCRYGG 1 cut(s) 303
BtsCI GGATG 2 cut(s) 180, 258
BtsI GCAGTG 1 cut(s) 133
BtsIMutI CAGTG 1 cut(s) 133
BveI ACCTGC 2 cut(s) 65, 125
Cac8I GCNNGC 5 cut(s) 150, 191, 195, 279, 358
CciI TCATGA 1 cut(s) 610
CfoI GCGC 7 cut(s) 92, 140, 159, 168, 283, 297, 434
Cfr10I RCCGGY 1 cut(s) 14
Cfr13I GGNCC 3 cut(s) 21, 780, 794
Cfr9I CCCGGG 1 cut(s) 464
CviAII CATG 1 cut(s) 611
DdeI CTNAG 1 cut(s) 709
DpnI GATC 2 cut(s) 131, 524
DpnII GATC 2 cut(s) 129, 522
EaeI YGGCCR 3 cut(s) 75, 201, 324
EagI CGGCCG 3 cut(s) 75, 201, 324
Eam1104I CTCTTC 1 cut(s) 658
EarI CTCTTC 1 cut(s) 658
EciI GGCGGA 1 cut(s) 256
EclXI CGGCCG 3 cut(s) 75, 201, 324
Eco24I GRGCYC 1 cut(s) 36
Eco47I GGWCC 3 cut(s) 21, 780, 794
Eco52I CGGCCG 3 cut(s) 75, 201, 324
Eco57I CTGAAG 1 cut(s) 646
Eco72I CACGTG 2 cut(s) 213, 246
Eco88I CYCGRG 1 cut(s) 464
EcoRII CCWGG 1 cut(s) 796
EcoT38I GRGCYC 1 cut(s) 36
Esp3I CGTCTC 2 cut(s) 700, 767
FaeI CATG 1 cut(s) 614
FaiI YATR 7 cut(s) 473, 545, 612, 771, 834, 838, 855
FaqI GGGAC 4 cut(s) 18, 284, 793, 807
FatI CATG 1 cut(s) 610
Fnu4HI GCNGC 5 cut(s) 88, 204, 228, 231, 354
FokI GGATG 2 cut(s) 187, 245
FriOI GRGCYC 1 cut(s) 36
Fsp4HI GCNGC 5 cut(s) 88, 204, 228, 231, 354
FspBI CTAG 1 cut(s) 317
GlaI GCGC 7 cut(s) 91, 139, 158, 167, 282, 296, 433
GluI GCNGC 5 cut(s) 88, 204, 228, 231, 354
GsaI CCCAGC 1 cut(s) 790
GsuI CTGGAG 1 cut(s) 517
HaeII RGCGCY 1 cut(s) 435
HaeIII GGCC 5 cut(s) 77, 101, 203, 326, 360
HapII CCGG 6 cut(s) 15, 29, 200, 465, 482, 560
HhaI GCGC 7 cut(s) 92, 140, 159, 168, 283, 297, 434
Hin1I GRCGYC 6 cut(s) 225, 252, 369, 396, 816, 825
Hin1II CATG 1 cut(s) 614
Hin6I GCGC 7 cut(s) 90, 138, 157, 166, 281, 295, 432
HinP1I GCGC 7 cut(s) 90, 138, 157, 166, 281, 295, 432
HincII GTYRAC 1 cut(s) 598
HindII GTYRAC 1 cut(s) 598
HinfI GANTC 7 cut(s) 95, 122, 320, 329, 401, 491, 577
HpaII CCGG 6 cut(s) 15, 29, 200, 465, 482, 560
HphI GGTGA 1 cut(s) 28
Hpy166II GTNNAC 1 cut(s) 598
Hpy188I TCNGA 1 cut(s) 748
Hpy188III TCNNGA 3 cut(s) 452, 611, 728
Hpy8I GTNNAC 1 cut(s) 598
HpyAV CCTTC 1 cut(s) 670
HpyCH4IV ACGT 3 cut(s) 212, 245, 825
HpyCH4V TGCA 3 cut(s) 60, 216, 639
HpyF3I CTNAG 1 cut(s) 709
HpySE526I ACGT 3 cut(s) 212, 245, 825
Hsp92I GRCGYC 6 cut(s) 225, 252, 369, 396, 816, 825
Hsp92II CATG 1 cut(s) 614
HspAI GCGC 7 cut(s) 90, 138, 157, 166, 281, 295, 432
Kzo9I GATC 2 cut(s) 129, 522
LmnI GCTCC 3 cut(s) 202, 307, 802
Lsp1109I GCAGC 1 cut(s) 340
LweI GCATC 1 cut(s) 436
MaeI CTAG 1 cut(s) 317
MaeII ACGT 3 cut(s) 212, 245, 825
MaeIII GTNAC 2 cut(s) 208, 241
MalI GATC 2 cut(s) 131, 524
MbiI CCGCTC 1 cut(s) 277
MboI GATC 2 cut(s) 129, 522
MboII GAAGA 4 cut(s) 73, 404, 469, 645
MflI RGATCY 1 cut(s) 129
MhlI GDGCHC 1 cut(s) 36
MluCI AATT 4 cut(s) 424, 616, 736, 807
MlyI GAGTC 4 cut(s) 89, 116, 314, 338
MseI TTAA 1 cut(s) 384
MspA1I CMGCKG 1 cut(s) 786
MspI CCGG 6 cut(s) 15, 29, 200, 465, 482, 560
MspR9I CCNGG 5 cut(s) 30, 465, 466, 482, 798
MvaI CCWGG 1 cut(s) 798
MvnI CGCG 7 cut(s) 90, 152, 157, 168, 281, 283, 297
NciI CCSGG 4 cut(s) 30, 465, 466, 482
NdeII GATC 2 cut(s) 129, 522
NlaIII CATG 1 cut(s) 614
NlaIV GGNNCC 5 cut(s) 23, 309, 558, 781, 795
NmeAIII GCCGAG 4 cut(s) 77, 302, 329, 352
NmuCI GTSAC 2 cut(s) 208, 241
NspV TTCGAA 1 cut(s) 501
PagI TCATGA 1 cut(s) 610
PaqCI CACCTGC 1 cut(s) 65
PcsI WCGNNNNNNNCGW 1 cut(s) 396
PfeI GAWTC 3 cut(s) 401, 491, 577
PflFI GACNNNGTC 1 cut(s) 815
PfoI TCCNGGA 1 cut(s) 480
PkrI GCNGC 5 cut(s) 89, 205, 229, 232, 355
PleI GAGTC 4 cut(s) 89, 116, 314, 337
PmaCI CACGTG 2 cut(s) 213, 246
PmlI CACGTG 2 cut(s) 213, 246
PpsI GAGTC 4 cut(s) 89, 116, 314, 337
Ppu21I YACGTR 2 cut(s) 213, 246
Psp6I CCWGG 1 cut(s) 796
PspCI CACGTG 2 cut(s) 213, 246
PspFI CCCAGC 1 cut(s) 786
PspGI CCWGG 1 cut(s) 796
PspN4I GGNNCC 5 cut(s) 23, 309, 558, 781, 795
PspPI GGNCC 3 cut(s) 21, 780, 794
PstI CTGCAG 1 cut(s) 641
PsuI RGATCY 1 cut(s) 129
PsyI GACNNNGTC 1 cut(s) 815
PvuII CAGCTG 1 cut(s) 786
SaqAI TTAA 1 cut(s) 384
SatI GCNGC 5 cut(s) 88, 204, 228, 231, 354
Sau3AI GATC 2 cut(s) 129, 522
Sau96I GGNCC 3 cut(s) 21, 780, 794
SchI GAGTC 4 cut(s) 89, 116, 314, 338
ScrFI CCNGG 5 cut(s) 30, 465, 466, 482, 798
SduI GDGCHC 1 cut(s) 36
SfaNI GCATC 1 cut(s) 436
SfcI CTRYAG 1 cut(s) 637
SfuI TTCGAA 1 cut(s) 501
SinI GGWCC 3 cut(s) 21, 780, 794
SmaI CCCGGG 1 cut(s) 466
Sse9I AATT 4 cut(s) 424, 616, 736, 807
SsiI CCGC 6 cut(s) 88, 204, 228, 231, 267, 275
SspMI CTAG 1 cut(s) 317
StyD4I CCNGG 5 cut(s) 28, 463, 464, 480, 796
TaiI ACGT 3 cut(s) 215, 248, 828
TaqI TCGA 4 cut(s) 453, 501, 507, 624
TasI AATT 4 cut(s) 424, 616, 736, 807
TauI GCSGC 4 cut(s) 90, 206, 230, 233
TfiI GAWTC 3 cut(s) 401, 491, 577
Tru1I TTAA 1 cut(s) 384
Tru9I TTAA 1 cut(s) 384
TscAI CASTG 1 cut(s) 140
TseFI GTSAC 2 cut(s) 208, 241
TseI GCWGC 1 cut(s) 353
Tsp45I GTSAC 2 cut(s) 208, 241
TspDTI ATGAA 4 cut(s) 430, 627, 729, 842
TspGWI ACGGA 1 cut(s) 747
TspMI CCCGGG 1 cut(s) 464
TspRI CASTG 1 cut(s) 140
Tth111I GACNNNGTC 1 cut(s) 815
VpaK11BI GGWCC 3 cut(s) 21, 780, 794
XapI RAATTY 2 cut(s) 424, 736
XcmI CCANNNNNNNNNTGG 1 cut(s) 541
XmaI CCCGGG 1 cut(s) 464
XspI CTAG 1 cut(s) 317
ZraI GACGTC 1 cut(s) 826
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.