pycom10g08090

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0002668
Physical Location & Seq
Forward (+)
10398307 .. 10403064
4758 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g08090.1

Sequence Viewer

Length: 2061 bp
ATGCAAGGTCAAGGGAGTGGTATTGATACTGTCATTGAAATCACAGATGTTGAGCTTGGATCTCTCTCAAACAGCACTAGTATGAGTCAGCAGACTTCTTTGATTAATATGCTAAATCCTGTTGAAAACCGGTTGTCAAGTTATATGGTAACATCTGGTGAGATTCCATGTTTAAATGCCTCTAGTAACCAGAACTTGAGTGGTTCAGGTGAACCTAGCTCTAGATTGGGTCCAGTTGATGATGATGGGATGAAATTGGGACAAGGTTGTTCATCTCCTTATAGTGCTTGTCCTGTGATTGGTCAAAGTTCAGAAGAAAGACGAGTTGAACAAAATATCCTTTTTCCTGGGAGAGCTCTTATTGGAATCAGTGGCAATCAGATTCAAGGTGGGCCATTTTTTTCTCAGGGCTTTCAAAATGAAAATATAAATTCTGGATATGTGGGAAGTAGTGGTATTAGTGTGCCTGGCATGGAGGATGATATTTCAGGTGGATTAGAAACAGAACAGACATCTTCTGGTAGTGCTTCACCCAATATTGTTGGGACTTTATCCAGAAGCACTGATTTTCTGGTTGAGGAAAATGATGGCAACCCAAGTTCTTCTTTTGGATCTTGGGGTTTATCATGCAAGCGAAAGGCCCTTGAGGGTACTTCAGGCCAGGCTTATTCTGGTGGAAGTTCTAGCTCCCTTCCACAAGCTGAAACTAGTGCTTGGAATACTGGTTCTGCTCGTTGTAGTCCTAGTAATTTAAGTTTATCTACACTGTTGCGTAGTTCTCCTAGTGGTGGTCCTTCAGAACAAAACTCAAGAACCAGGGTTGGTTTGAGACTTGGGGCATCTGATACCTTTCCTTCAATAGATGCAACAGGAAATGGAGCAAGCTCACTGCGGGAATTTTTTTGTAGGGGGGCTTGTTCTGGGCCTCAGCAAGAATCTGCTCCATCCAACTTATCATCACAAGGGAGGTCAATAACTGCAGCTGCAACAAATTCGGCTGGTCCCCAAAGCCAGTTTCCTGCAATGCATATGAATGGTTCGTCAGGAAACATGCTTCCTTTTCCTTGGAATGGTGCTTCCAGTTCAAGAGTGGGCAGCTTACCAAGTTCTCTTTTGTCTGGATACAGAGGTGCTGAGTTAAGAGGAGAAGCAAACTTAAGAAGCATTCCCCGAAACAATGTGGAGCATTCGACTTATGCAACTGCCAATGATATGAGAAATTCAGCACAGGATCCCACAATTTGGGGTCTGGCCTCTGGAGAATTAAGCATTTCTGTAGGTGCCCCTTCCTCTTCAAGAAGTGGCCCCAGTTCTGGCATTCGTTTTGTGGCTACTCCTGCATTGATCCGTGCCAACAACTCTCCATCTGAAAATCATCAAAGTACAATAGATTGGTCTTTGTTTCCTTCTTTTGATTCTCAACCCGGAGGTCATAGTCATTATAACCCAGTACCTTCAGGACCTGCTTCCGCACAGGATTCAGGAAGTTCTTCTGGGTCTAATAACCAGGCTCGTCACTTACCACATCCTAGGTCCACATTCTTGGACAGACATGGTGATGATGCTCTTAATATGCCCCAGTCACTGCGGGCATTAGCAGCTGACATCGAAGGGAGGCGCCGACTTATATCTGAGATTCGTCAAGTTTTGAATGCTATGCGTAGGGGTGAGAACTTACGCGCCGAGGATTATATGCTCTTTGACCCATTCATATATCATGGCATGGCTGAGATGCACGACAGGCATAGAGACATGCGCCTTGATGTTGATAACATGTCTTATGAGGAACTGCTGGCACTGGGGGAACGTATAGGAGATGTTAGCACTGGACTGAGTGAGGAGACTATTATGAAGTGCATGAAACAGCGAAAATTTCTCTGTATTTCAGAAAAATCCCCTGCAGATGTGGAACCATGCTGTGTCTGTCAGGAGGCTTTTGCTGATGAGGATGATATTGGCACACTGGATTGTGGGCATGACTTCCACAACAGTTGCATCAAGCAATGGCTAATGCAGAAGAATCTGTGCCCGATTTGTAAAACAACAGCCCTGCTTACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000209 GO:0000902 GO:0000904 GO:0003006 GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006355 GO:0006357 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0006928 GO:0006935 GO:0007275 GO:0007399 GO:0007409 GO:0007411 GO:0007568 GO:0008045 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009605 GO:0009653 GO:0009791 GO:0009889 GO:0009966 GO:0009967 GO:0009987 GO:0010228 GO:0010259 GO:0010468 GO:0010498 GO:0010556 GO:0010646 GO:0010647 GO:0016043 GO:0016567 GO:0016740 GO:0019219 GO:0019222 GO:0019538 GO:0019787 GO:0019941 GO:0021675 GO:0022008 GO:0022414 GO:0023051 GO:0023056 GO:0030030 GO:0030154 GO:0030163 GO:0030182 GO:0030510 GO:0030513 GO:0031175 GO:0031323 GO:0031326 GO:0032446 GO:0032501 GO:0032502 GO:0032989 GO:0032990 GO:0032991 GO:0035107 GO:0035108 GO:0035136 GO:0036211 GO:0040011 GO:0042221 GO:0042330 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044464 GO:0048468 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048608 GO:0048666 GO:0048667 GO:0048699 GO:0048731 GO:0048736 GO:0048812 GO:0048856 GO:0048858 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0051603 GO:0060173 GO:0060255 GO:0060384 GO:0061061 GO:0061458 GO:0061564 GO:0061630 GO:0061659 GO:0065007 GO:0070647 GO:0071704 GO:0071840 GO:0080090 GO:0090092 GO:0090100 GO:0090287 GO:0097485 GO:0120036 GO:0120039 GO:0140096 GO:1901564 GO:1901565 GO:1901575 GO:1903506 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

687

Amino Acids

72.76

Weight (kDa)

5.11

Isoelectric Point (pI)

63.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 637 - 680 3.2e-08 RING-H2 zinc finger domain
zf-RING_2 PF13639 639 - 680 1.6e-11 Ring finger domain
zf-C3HC4_2 PF13923 639 - 679 2.8e-07 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 639 - 679 1.4e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1443
Acc36I ACCTGC 1 cut(s) 1471
AccB1I GGYRCC 2 cut(s) 1280, 1617
AccB7I CCANNNNNTGG 1 cut(s) 1242
AccII CGCG 1 cut(s) 1680
AciI CCGC 3 cut(s) 892, 1470, 1588
AclWI GGATC 5 cut(s) 67, 619, 1226, 1239, 1339
AcsI RAATTY 5 cut(s) 430, 896, 991, 1219, 1871
AcuI CTGAAG 3 cut(s) 639, 780, 1440
AcyI GRCGYC 1 cut(s) 1618
AfaI GTAC 3 cut(s) 652, 1384, 1452
AfiI CCNNNNNNNGG 5 cut(s) 299, 788, 1070, 1242, 1313
AflII CTTAAG 1 cut(s) 1156
AflIII ACRYGT 1 cut(s) 1773
AgeI ACCGGT 1 cut(s) 129
AgsI TTSAA 9 cut(s) 38, 125, 329, 386, 416, 858, 1086, 1296, 1651
AhlI ACTAGT 2 cut(s) 77, 707
AjnI CCWGG 5 cut(s) 346, 466, 660, 815, 1506
AjuI GAANNNNNNNTTGG 2 cut(s) 999, 1031
AloI GAACNNNNNNTCC 4 cut(s) 321, 353, 709, 741
AluBI AGCT 9 cut(s) 55, 219, 356, 687, 701, 885, 983, 1098, 1601
AluI AGCT 9 cut(s) 55, 219, 356, 687, 701, 885, 983, 1098, 1601
Alw21I GWGCWC 1 cut(s) 358
Alw26I GTCTC 3 cut(s) 823, 1743, 1835
AlwI GGATC 5 cut(s) 67, 619, 1226, 1239, 1339
AlwNI CAGNNNCTG 2 cut(s) 1463, 1585
AoxI GGCC 6 cut(s) 392, 639, 658, 923, 1251, 1303
ApeKI GCWGC 4 cut(s) 980, 983, 1095, 1598
ApoI RAATTY 5 cut(s) 430, 896, 991, 1219, 1871
AseI ATTAAT 1 cut(s) 105
AsiGI ACCGGT 1 cut(s) 129
Asp700I GAANNNNTTC 1 cut(s) 1489
AspA2I CCTAGG 1 cut(s) 1529
AspLEI GCGC 3 cut(s) 1620, 1682, 1758
AspS9I GGNCC 9 cut(s) 230, 392, 640, 791, 923, 1001, 1304, 1460, 1533
AsuC2I CCSGG 1 cut(s) 1425
AsuHPI GGTGA 5 cut(s) 170, 221, 522, 1568, 1679
AvaII GGWCC 5 cut(s) 230, 791, 1001, 1460, 1533
AvrII CCTAGG 1 cut(s) 1529
BaeGI GKGCMC 2 cut(s) 1285, 2030
BaeI ACNNNNGTAYC 2 cut(s) 1114, 1147
BamHI GGATCC 1 cut(s) 1231
BanI GGYRCC 2 cut(s) 1280, 1617
BanII GRGCYC 1 cut(s) 358
Bbv12I GWGCWC 1 cut(s) 358
BbvCI CCTCAGC 1 cut(s) 927
BbvI GCAGC 4 cut(s) 970, 992, 1107, 1610
BccI CCATC 4 cut(s) 239, 581, 952, 1372
BcgI CGANNNNNNTGC 2 cut(s) 975, 1009
BciT130I CCWGG 5 cut(s) 348, 468, 662, 817, 1508
BciVI GTATCC 1 cut(s) 1115
BcnI CCSGG 1 cut(s) 1425
BcoDI GTCTC 3 cut(s) 823, 1743, 1835
BcuI ACTAGT 2 cut(s) 77, 707
BfaI CTAG 9 cut(s) 78, 183, 216, 222, 684, 708, 744, 783, 1530
BfmI CTRYAG 3 cut(s) 978, 1275, 1899
BfoI RGCGCY 1 cut(s) 1621
BfrI CTTAAG 1 cut(s) 1156
BfuAI ACCTGC 1 cut(s) 1471
BfuI GTATCC 1 cut(s) 1115
BisI GCNGC 4 cut(s) 981, 984, 1096, 1599
BlnI CCTAGG 1 cut(s) 1529
BlsI GCNGC 4 cut(s) 982, 985, 1097, 1600
Bme1390I CCNGG 6 cut(s) 348, 468, 662, 817, 1425, 1508
Bme18I GGWCC 5 cut(s) 230, 791, 1001, 1460, 1533
BmgT120I GGNCC 9 cut(s) 230, 392, 640, 791, 923, 1001, 1304, 1460, 1533
BmiI GGNNCC 7 cut(s) 231, 1003, 1233, 1282, 1306, 1619, 1911
BmrFI CCNGG 6 cut(s) 348, 468, 662, 817, 1425, 1508
BmrI ACTGGG 4 cut(s) 1302, 1442, 1573, 1808
BmsI GCATC 5 cut(s) 848, 853, 1552, 1722, 2004
BmuI ACTGGG 4 cut(s) 1302, 1442, 1573, 1808
BpmI CTGGAG 1 cut(s) 1278
Bpu10I CCTNAGC 1 cut(s) 927
BpuEI CTTGAG 3 cut(s) 217, 665, 793
BpuMI CCSGG 1 cut(s) 1425
BsaHI GRCGYC 1 cut(s) 1618
BsaJI CCNNGG 5 cut(s) 347, 816, 1064, 1529, 1683
BsaWI WCCGGW 1 cut(s) 129
Bsc4I CCNNNNNNNGG 5 cut(s) 299, 788, 1070, 1242, 1313
Bse118I RCCGGY 1 cut(s) 129
Bse3DI GCAATG 2 cut(s) 1029, 2009
BseBI CCWGG 5 cut(s) 348, 468, 662, 817, 1508
BseDI CCNNGG 5 cut(s) 347, 816, 1064, 1529, 1683
BseGI GGATG 5 cut(s) 255, 484, 944, 1525, 1954
BseLI CCNNNNNNNGG 5 cut(s) 299, 788, 1070, 1242, 1313
BseMI GCAATG 2 cut(s) 1029, 2009
BseMII CTCAG 6 cut(s) 419, 941, 1125, 1623, 1719, 1823
BseRI GAGGAG 2 cut(s) 1158, 1853
BseSI GKGCMC 2 cut(s) 1285, 2030
BseXI GCAGC 4 cut(s) 970, 992, 1107, 1610
Bsh1236I CGCG 1 cut(s) 1680
BshFI GGCC 6 cut(s) 394, 641, 660, 925, 1253, 1305
BshNI GGYRCC 2 cut(s) 1280, 1617
BshTI ACCGGT 1 cut(s) 129
BsiHKAI GWGCWC 1 cut(s) 358
BsiSI CCGG 2 cut(s) 130, 1425
BslFI GGGAC 3 cut(s) 273, 559, 987
BslI CCNNNNNNNGG 5 cut(s) 299, 788, 1070, 1242, 1313
BsmAI GTCTC 3 cut(s) 823, 1743, 1835
BsmFI GGGAC 3 cut(s) 273, 559, 987
BsmI GAATGC 4 cut(s) 1164, 1186, 1317, 1657
BsnI GGCC 6 cut(s) 394, 641, 660, 925, 1253, 1305
Bsp1286I GDGCHC 3 cut(s) 358, 1285, 2030
Bsp143I GATC 4 cut(s) 59, 611, 1231, 1344
BspACI CCGC 3 cut(s) 892, 1470, 1588
BspANI GGCC 6 cut(s) 394, 641, 660, 925, 1253, 1305
BspCNI CTCAG 6 cut(s) 418, 940, 1126, 1624, 1720, 1824
BspFNI CGCG 1 cut(s) 1680
BspLI GGNNCC 7 cut(s) 231, 1003, 1233, 1282, 1306, 1619, 1911
BspMAI CTGCAG 2 cut(s) 982, 1903
BspMI ACCTGC 1 cut(s) 1471
BspPI GGATC 5 cut(s) 67, 619, 1226, 1239, 1339
BspT107I GGYRCC 2 cut(s) 1280, 1617
BspTI CTTAAG 1 cut(s) 1156
BsrDI GCAATG 2 cut(s) 1029, 2009
BsrFI RCCGGY 1 cut(s) 129
BssAI RCCGGY 1 cut(s) 129
BssECI CCNNGG 5 cut(s) 347, 816, 1064, 1529, 1683
BssMI GATC 4 cut(s) 59, 611, 1231, 1344
BssNI GRCGYC 1 cut(s) 1618
BssT1I CCWWGG 2 cut(s) 1064, 1529
Bst2UI CCWGG 5 cut(s) 348, 468, 662, 817, 1508
Bst4CI ACNGT 3 cut(s) 31, 768, 1991
Bst6I CTCTTC 1 cut(s) 1297
BstACI GRCGYC 1 cut(s) 1618
BstAFI CTTAAG 1 cut(s) 1156
BstC8I GCNNGC 4 cut(s) 632, 883, 1590, 1794
BstDEI CTNAG 6 cut(s) 405, 927, 1134, 1632, 1728, 1832
BstF5I GGATG 5 cut(s) 255, 484, 944, 1525, 1954
BstFNI CGCG 1 cut(s) 1680
BstH2I RGCGCY 1 cut(s) 1621
BstHHI GCGC 3 cut(s) 1620, 1682, 1758
BstKTI GATC 4 cut(s) 62, 614, 1234, 1347
BstMAI GTCTC 3 cut(s) 823, 1743, 1835
BstMBI GATC 4 cut(s) 59, 611, 1231, 1344
BstMWI GCNNNNNNNGC 3 cut(s) 1337, 1598, 1741
BstNI CCWGG 5 cut(s) 348, 468, 662, 817, 1508
BstNSI RCATGY 3 cut(s) 1054, 1756, 1777
BstSCI CCNGG 6 cut(s) 346, 466, 660, 815, 1423, 1506
BstSFI CTRYAG 3 cut(s) 978, 1275, 1899
BstSLI GKGCMC 2 cut(s) 1285, 2030
BstUI CGCG 1 cut(s) 1680
BstV1I GCAGC 4 cut(s) 970, 992, 1107, 1610
BstX2I RGATCY 3 cut(s) 59, 611, 1231
BstXI CCANNNNNNTGG 1 cut(s) 1543
BstYI RGATCY 3 cut(s) 59, 611, 1231
BsuI GTATCC 1 cut(s) 1115
BsuRI GGCC 6 cut(s) 394, 641, 660, 925, 1253, 1305
BtsCI GGATG 5 cut(s) 255, 484, 944, 1525, 1954
BtsI GCAGTG 2 cut(s) 887, 1583
BtsIMutI CAGTG 8 cut(s) 376, 561, 764, 887, 1583, 1796, 1824, 1961
BveI ACCTGC 1 cut(s) 1471
Cac8I GCNNGC 4 cut(s) 632, 883, 1590, 1794
CaiI CAGNNNCTG 2 cut(s) 1463, 1585
CfoI GCGC 3 cut(s) 1620, 1682, 1758
Cfr10I RCCGGY 1 cut(s) 129
Cfr13I GGNCC 9 cut(s) 230, 392, 640, 791, 923, 1001, 1304, 1460, 1533
Csp6I GTAC 3 cut(s) 651, 1383, 1451
CspAI ACCGGT 1 cut(s) 129
CspCI CAANNNNNGTGG 2 cut(s) 1973, 2008
CviQI GTAC 3 cut(s) 651, 1383, 1451
DdeI CTNAG 6 cut(s) 405, 927, 1134, 1632, 1728, 1832
DinI GGCGCC 1 cut(s) 1619
DpnI GATC 4 cut(s) 61, 613, 1233, 1346
DpnII GATC 4 cut(s) 59, 611, 1231, 1344
DraI TTTAAA 1 cut(s) 174
Eam1104I CTCTTC 1 cut(s) 1297
EarI CTCTTC 1 cut(s) 1297
Ecl136II GAGCTC 1 cut(s) 356
Eco130I CCWWGG 2 cut(s) 1064, 1529
Eco24I GRGCYC 1 cut(s) 358
Eco47I GGWCC 5 cut(s) 230, 791, 1001, 1460, 1533
Eco53kI GAGCTC 1 cut(s) 356
Eco57I CTGAAG 3 cut(s) 639, 780, 1440
EcoICRI GAGCTC 1 cut(s) 356
EcoO109I RGGNCCY 2 cut(s) 640, 1460
EcoRII CCWGG 5 cut(s) 346, 466, 660, 815, 1506
EcoT14I CCWWGG 2 cut(s) 1064, 1529
EcoT22I ATGCAT 1 cut(s) 1029
EcoT38I GRGCYC 1 cut(s) 358
EgeI GGCGCC 1 cut(s) 1619
EheI GGCGCC 1 cut(s) 1619
ErhI CCWWGG 2 cut(s) 1064, 1529
FalI AAGNNNNNCTT 2 cut(s) 589, 621
FaqI GGGAC 3 cut(s) 273, 559, 987
FauI CCCGC 2 cut(s) 885, 1581
FauNDI CATATG 1 cut(s) 1029
Fnu4HI GCNGC 4 cut(s) 981, 984, 1096, 1599
FokI GGATG 5 cut(s) 262, 491, 931, 1512, 1961
FriOI GRGCYC 1 cut(s) 358
Fsp4HI GCNGC 4 cut(s) 981, 984, 1096, 1599
FspBI CTAG 9 cut(s) 78, 183, 216, 222, 684, 708, 744, 783, 1530
GlaI GCGC 3 cut(s) 1619, 1681, 1757
GluI GCNGC 4 cut(s) 981, 984, 1096, 1599
GsuI CTGGAG 1 cut(s) 1278
HaeII RGCGCY 1 cut(s) 1621
HaeIII GGCC 6 cut(s) 394, 641, 660, 925, 1253, 1305
HapII CCGG 2 cut(s) 130, 1425
HhaI GCGC 3 cut(s) 1620, 1682, 1758
Hin1I GRCGYC 1 cut(s) 1618
Hin6I GCGC 3 cut(s) 1618, 1680, 1756
HinP1I GCGC 3 cut(s) 1618, 1680, 1756
HinfI GANTC 9 cut(s) 85, 163, 366, 382, 935, 1415, 1478, 1636, 2020
HpaII CCGG 2 cut(s) 130, 1425
HphI GGTGA 5 cut(s) 170, 221, 522, 1568, 1679
Hpy166II GTNNAC 2 cut(s) 212, 1536
Hpy188I TCNGA 7 cut(s) 313, 381, 799, 844, 1369, 1633, 1888
Hpy8I GTNNAC 2 cut(s) 212, 1536
HpyAV CCTTC 7 cut(s) 701, 804, 864, 1296, 1416, 1464, 1604
HpyCH4III ACNGT 3 cut(s) 31, 768, 1991
HpyCH4IV ACGT 1 cut(s) 1807
HpyF10VI GCNNNNNNNGC 3 cut(s) 1337, 1598, 1741
HpyF3I CTNAG 6 cut(s) 405, 927, 1134, 1632, 1728, 1832
HpySE526I ACGT 1 cut(s) 1807
Hsp92I GRCGYC 1 cut(s) 1618
HspAI GCGC 3 cut(s) 1618, 1680, 1756
KasI GGCGCC 1 cut(s) 1617
Kzo9I GATC 4 cut(s) 59, 611, 1231, 1344
LmnI GCTCC 4 cut(s) 692, 878, 946, 1183
Lsp1109I GCAGC 4 cut(s) 970, 992, 1107, 1610
LweI GCATC 5 cut(s) 848, 853, 1552, 1722, 2004
MaeI CTAG 9 cut(s) 78, 183, 216, 222, 684, 708, 744, 783, 1530
MaeII ACGT 1 cut(s) 1807
MaeIII GTNAC 4 cut(s) 148, 185, 1514, 1581
MalI GATC 4 cut(s) 61, 613, 1233, 1346
MboI GATC 4 cut(s) 59, 611, 1231, 1344
MboII GAAGA 6 cut(s) 326, 507, 594, 1284, 1482, 2029
MflI RGATCY 3 cut(s) 59, 611, 1231
MhlI GDGCHC 3 cut(s) 358, 1285, 2030
MluCI AATT 9 cut(s) 254, 430, 748, 896, 991, 1219, 1239, 1262, 1871
Mly113I GGCGCC 1 cut(s) 1618
MlyI GAGTC 1 cut(s) 94
MmeI TCCRAC 1 cut(s) 972
Mph1103I ATGCAT 1 cut(s) 1029
MroXI GAANNNNTTC 1 cut(s) 1489
MseI TTAA 7 cut(s) 105, 173, 752, 1139, 1157, 1265, 1569
MslI CAYNNNNRTG 3 cut(s) 80, 1032, 1557
MspA1I CMGCKG 2 cut(s) 983, 1601
MspCI CTTAAG 1 cut(s) 1156
MspI CCGG 2 cut(s) 130, 1425
MspR9I CCNGG 6 cut(s) 348, 468, 662, 817, 1425, 1508
Mva1269I GAATGC 4 cut(s) 1164, 1186, 1317, 1657
MvaI CCWGG 5 cut(s) 348, 468, 662, 817, 1508
MvnI CGCG 1 cut(s) 1680
MwoI GCNNNNNNNGC 3 cut(s) 1337, 1598, 1741
NarI GGCGCC 1 cut(s) 1618
NciI CCSGG 1 cut(s) 1425
NdeI CATATG 1 cut(s) 1029
NdeII GATC 4 cut(s) 59, 611, 1231, 1344
NlaIV GGNNCC 7 cut(s) 231, 1003, 1233, 1282, 1306, 1619, 1911
NmeAIII GCCGAG 1 cut(s) 1708
NmuCI GTSAC 2 cut(s) 1514, 1581
NsiI ATGCAT 1 cut(s) 1029
NspI RCATGY 3 cut(s) 1054, 1756, 1777
PciI ACATGT 1 cut(s) 1773
PctI GAATGC 4 cut(s) 1164, 1186, 1317, 1657
PdmI GAANNNNTTC 1 cut(s) 1489
PfeI GAWTC 8 cut(s) 163, 366, 382, 935, 1415, 1478, 1636, 2020
PflMI CCANNNNNTGG 1 cut(s) 1242
PinAI ACCGGT 1 cut(s) 129
PkrI GCNGC 4 cut(s) 982, 985, 1097, 1600
PleI GAGTC 1 cut(s) 93
PluTI GGCGCC 1 cut(s) 1621
PpsI GAGTC 1 cut(s) 93
PpuMI RGGWCCY 1 cut(s) 1460
PscI ACATGT 1 cut(s) 1773
PshBI ATTAAT 1 cut(s) 105
PsiI TTATAA 1 cut(s) 1443
Psp124BI GAGCTC 1 cut(s) 358
Psp5II RGGWCCY 1 cut(s) 1460
Psp6I CCWGG 5 cut(s) 346, 466, 660, 815, 1506
PspGI CCWGG 5 cut(s) 346, 466, 660, 815, 1506
PspN4I GGNNCC 7 cut(s) 231, 1003, 1233, 1282, 1306, 1619, 1911
PspPI GGNCC 9 cut(s) 230, 392, 640, 791, 923, 1001, 1304, 1460, 1533
PspPPI RGGWCCY 1 cut(s) 1460
PstI CTGCAG 2 cut(s) 982, 1903
PstNI CAGNNNCTG 2 cut(s) 1463, 1585
PsuI RGATCY 3 cut(s) 59, 611, 1231
PvuII CAGCTG 2 cut(s) 983, 1601
RsaI GTAC 3 cut(s) 652, 1384, 1452
RsaNI GTAC 3 cut(s) 651, 1383, 1451
RseI CAYNNNNRTG 3 cut(s) 80, 1032, 1557
SacI GAGCTC 1 cut(s) 358
SaqAI TTAA 7 cut(s) 105, 173, 752, 1139, 1157, 1265, 1569
SatI GCNGC 4 cut(s) 981, 984, 1096, 1599
Sau3AI GATC 4 cut(s) 59, 611, 1231, 1344
Sau96I GGNCC 9 cut(s) 230, 392, 640, 791, 923, 1001, 1304, 1460, 1533
SchI GAGTC 1 cut(s) 94
ScrFI CCNGG 6 cut(s) 348, 468, 662, 817, 1425, 1508
SduI GDGCHC 3 cut(s) 358, 1285, 2030
SfaNI GCATC 5 cut(s) 848, 853, 1552, 1722, 2004
SfcI CTRYAG 3 cut(s) 978, 1275, 1899
SfoI GGCGCC 1 cut(s) 1619
SinI GGWCC 5 cut(s) 230, 791, 1001, 1460, 1533
SmiMI CAYNNNNRTG 3 cut(s) 80, 1032, 1557
SmlI CTYRAG 4 cut(s) 196, 644, 808, 1156
SmoI CTYRAG 4 cut(s) 196, 644, 808, 1156
SpeI ACTAGT 2 cut(s) 77, 707
Sse9I AATT 9 cut(s) 254, 430, 748, 896, 991, 1219, 1239, 1262, 1871
SsiI CCGC 3 cut(s) 892, 1470, 1588
SspDI GGCGCC 1 cut(s) 1617
SspI AATATT 1 cut(s) 538
SspMI CTAG 9 cut(s) 78, 183, 216, 222, 684, 708, 744, 783, 1530
SstI GAGCTC 1 cut(s) 358
StyD4I CCNGG 6 cut(s) 346, 466, 660, 815, 1423, 1506
StyI CCWWGG 2 cut(s) 1064, 1529
TaaI ACNGT 3 cut(s) 31, 768, 1991
TaiI ACGT 1 cut(s) 1810
TaqI TCGA 2 cut(s) 1190, 1608
TasI AATT 9 cut(s) 254, 430, 748, 896, 991, 1219, 1239, 1262, 1871
TatI WGTACW 1 cut(s) 1382
TfiI GAWTC 8 cut(s) 163, 366, 382, 935, 1415, 1478, 1636, 2020
Tru1I TTAA 7 cut(s) 105, 173, 752, 1139, 1157, 1265, 1569
Tru9I TTAA 7 cut(s) 105, 173, 752, 1139, 1157, 1265, 1569
TscAI CASTG 8 cut(s) 376, 568, 771, 894, 1590, 1803, 1831, 1968
TseFI GTSAC 2 cut(s) 1514, 1581
TseI GCWGC 4 cut(s) 980, 983, 1095, 1598
Tsp45I GTSAC 2 cut(s) 1514, 1581
TspDTI ATGAA 7 cut(s) 261, 266, 435, 1046, 1699, 1865, 1874
TspGWI ACGGA 1 cut(s) 1337
TspRI CASTG 8 cut(s) 376, 568, 771, 894, 1590, 1803, 1831, 1968
Van91I CCANNNNNTGG 1 cut(s) 1242
Vha464I CTTAAG 1 cut(s) 1156
VpaK11BI GGWCC 5 cut(s) 230, 791, 1001, 1460, 1533
VspI ATTAAT 1 cut(s) 105
XapI RAATTY 5 cut(s) 430, 896, 991, 1219, 1871
XbaI TCTAGA 1 cut(s) 221
XceI RCATGY 3 cut(s) 1054, 1756, 1777
XcmI CCANNNNNNNNNTGG 3 cut(s) 197, 668, 1087
XmaJI CCTAGG 1 cut(s) 1529
XmnI GAANNNNTTC 1 cut(s) 1489
XspI CTAG 9 cut(s) 78, 183, 216, 222, 684, 708, 744, 783, 1530
Zsp2I ATGCAT 1 cut(s) 1029
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.