Rorug05G0566100

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0002668
Physical Location & Seq
Forward (+)
75945088 .. 75949041
3954 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0566100.1

Sequence Viewer

Length: 1080 bp
ATGGCAGAAGCACCCCAGATTGCCATTCTTGGAGCTGGTACTTTTGTGAAGACCCAATACATTCCAAGGCTGGCTGAGATCTCTAACCTTGTCAACCTTAAAGCCATTTGGAGCCGTTCTGAGGAATCTGCTAGAGGTGCAGTTGAGATTGCTGGGAAACATTTTCCAGGAGTAGAATGCAAATGGGGTGATCAGGGTCTTGAGGAAATTATTGCAGATAGTTCAATTCTTGGTGTTGCTGTGGTTTTAGCTGGCCAAGCTCAGGTTGATTTCTCACTGAAGCTTCTCAAAGCAGGGAAGAATGTCCTTCAAGAGAAACCAGCAGCAGCTACTACAAGTGAATTGGAAACTGCAGTGTCAAGTTATAAATCTATTGTTGCTAATACTCCCAATAAACCAATCTGGGCAGTAGCAGAAAATTATCGATTTGAACCTGCTTTTGTTGAGGGCAAGAAACTAATGACTGAAATTGGAGATGTGATGAGCATCCAAGTTATTGTTGAAGGATCAATGAACAGTTCAAACCCTTACTTCTCAAGCTCTTGGAGGCGAGACTTTAATGGTGGTTTCATTCTGGACATGGGAGTACATTTCGTTGCAGGACTGAGGATGCTTGCTGGATGTGAGATAGTCTCGGTGTCAGCTACAACGTCACATGTCGACAAGACTTTGCCTCCCCCAGATAACATATCCTCTCTCTTTCAACTGGAGAATGGCTGTTCTGGGATTTTTGTGTTGGTGGTCTCCTCAAGGTCTCCCAAGATAGTTTGGCGATTTGTTGGCTTGAAGGGAACACTGGAAGTTGAGCGTGGAAACCAAGATGGACGACACGGCTATGTGGTCACATTCTATAGTGCTGATGGACAAAGGGAAAGCTCCTTCTACCAATTTAGAGGAGTGACTGAAGAATTCATCGCATTTATTAATGACATAAAGCAAGCCACTTTAAAGAAGGGAGCTGGCTATGAAGCTGAGCCTCGCATGTCATTTCTGGAAGGTGCTAGAGATGTTGCTGTTCTAGAGGCAATGCTTGAATCTGGAGGAAAGCAAGGGGCACCAGTCCATGTGAAGAAATTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000209 GO:0000902 GO:0000904 GO:0003006 GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006355 GO:0006357 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0006928 GO:0006935 GO:0007275 GO:0007399 GO:0007409 GO:0007411 GO:0007568 GO:0008045 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009605 GO:0009653 GO:0009791 GO:0009889 GO:0009966 GO:0009967 GO:0009987 GO:0010228 GO:0010259 GO:0010468 GO:0010498 GO:0010556 GO:0010646 GO:0010647 GO:0016043 GO:0016567 GO:0016740 GO:0019219 GO:0019222 GO:0019538 GO:0019787 GO:0019941 GO:0021675 GO:0022008 GO:0022414 GO:0023051 GO:0023056 GO:0030030 GO:0030154 GO:0030163 GO:0030182 GO:0030510 GO:0030513 GO:0031175 GO:0031323 GO:0031326 GO:0032446 GO:0032501 GO:0032502 GO:0032989 GO:0032990 GO:0032991 GO:0035107 GO:0035108 GO:0035136 GO:0036211 GO:0040011 GO:0042221 GO:0042330 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044464 GO:0048468 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048608 GO:0048666 GO:0048667 GO:0048699 GO:0048731 GO:0048736 GO:0048812 GO:0048856 GO:0048858 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0051603 GO:0060173 GO:0060255 GO:0060384 GO:0061061 GO:0061458 GO:0061564 GO:0061630 GO:0061659 GO:0065007 GO:0070647 GO:0071704 GO:0071840 GO:0080090 GO:0090092 GO:0090100 GO:0090287 GO:0097485 GO:0120036 GO:0120039 GO:0140096 GO:1901564 GO:1901565 GO:1901575 GO:1903506 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

359

Amino Acids

38.94

Weight (kDa)

5.86

Isoelectric Point (pI)

35.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GFO_IDH_MocA PF01408 7 - 120 1.9e-11 Oxidoreductase family, NAD-binding Rossmann fold
GFO_IDH_MocA_C3 PF22725 156 - 267 2.7e-13 GFO/IDH/MocA C-terminal domain
GFO_IDH_MocA_C PF02894 156 - 347 1.9e-10 Oxidoreductase family, C-terminal alpha/beta domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 366
Acc36I ACCTGC 1 cut(s) 442
AccB1I GGYRCC 1 cut(s) 1054
AccI GTMKAC 1 cut(s) 660
AclWI GGATC 1 cut(s) 514
AcoI YGGCCR 1 cut(s) 253
AcsI RAATTY 2 cut(s) 908, 1073
AcuI CTGAAG 2 cut(s) 299, 924
AfaI GTAC 2 cut(s) 40, 588
AfiI CCNNNNNNNGG 2 cut(s) 121, 262
AflIII ACRYGT 1 cut(s) 655
AgsI TTSAA 8 cut(s) 225, 311, 431, 503, 522, 704, 787, 1034
AjnI CCWGG 1 cut(s) 166
Alw26I GTCTC 4 cut(s) 546, 637, 748, 759
AlwI GGATC 1 cut(s) 514
AoxI GGCC 1 cut(s) 253
ApeKI GCWGC 2 cut(s) 323, 326
ApoI RAATTY 2 cut(s) 908, 1073
AseI ATTAAT 1 cut(s) 924
AsuHPI GGTGA 1 cut(s) 200
BaeGI GKGCMC 1 cut(s) 1057
BaeI ACNNNNGTAYC 2 cut(s) 30, 63
BalI TGGCCA 1 cut(s) 255
BanI GGYRCC 1 cut(s) 1054
BarI GAAGNNNNNNTAC 2 cut(s) 41, 73
BbsI GAAGAC 1 cut(s) 56
BbvI GCAGC 2 cut(s) 335, 338
BccI CCATC 2 cut(s) 815, 854
BceAI ACGGC 2 cut(s) 99, 847
BciT130I CCWGG 1 cut(s) 168
BclI TGATCA 1 cut(s) 190
BcoDI GTCTC 4 cut(s) 546, 637, 748, 759
BfaI CTAG 3 cut(s) 132, 1002, 1019
BfmI CTRYAG 2 cut(s) 351, 850
BfuAI ACCTGC 1 cut(s) 442
BglII AGATCT 1 cut(s) 78
BisI GCNGC 2 cut(s) 324, 327
BlpI GCTNAGC 1 cut(s) 972
BlsI GCNGC 2 cut(s) 325, 328
Bme1390I CCNGG 1 cut(s) 168
BmiI GGNNCC 2 cut(s) 113, 1056
BmrFI CCNGG 1 cut(s) 168
BmsI GCATC 2 cut(s) 495, 600
BpiI GAAGAC 1 cut(s) 56
BplI GAGNNNNNCTC 2 cut(s) 617, 649
BpmI CTGGAG 2 cut(s) 728, 1059
Bpu10I CCTNAGC 1 cut(s) 261
Bpu1102I GCTNAGC 1 cut(s) 972
BpuEI CTTGAG 3 cut(s) 221, 520, 733
Bsa29I ATCGAT 1 cut(s) 424
BsaBI GATNNNNATC 1 cut(s) 485
BsaI GGTCTC 2 cut(s) 748, 759
BsaJI CCNNGG 1 cut(s) 65
BsaXI ACNNNNNCTCC 2 cut(s) 658, 688
Bsc4I CCNNNNNNNGG 2 cut(s) 121, 262
Bse1I ACTGG 3 cut(s) 711, 801, 1058
Bse3DI GCAATG 1 cut(s) 1032
Bse8I GATNNNNATC 1 cut(s) 485
BseBI CCWGG 1 cut(s) 168
BseCI ATCGAT 1 cut(s) 424
BseDI CCNNGG 1 cut(s) 65
BseGI GGATG 3 cut(s) 486, 615, 626
BseJI GATNNNNATC 1 cut(s) 485
BseLI CCNNNNNNNGG 2 cut(s) 121, 262
BseMI GCAATG 1 cut(s) 1032
BseMII CTCAG 5 cut(s) 66, 111, 275, 596, 963
BseNI ACTGG 3 cut(s) 711, 801, 1058
BseRI GAGGAG 2 cut(s) 736, 909
BseSI GKGCMC 1 cut(s) 1057
BseXI GCAGC 2 cut(s) 335, 338
BseYI CCCAGC 1 cut(s) 152
BsgI GTGCAG 1 cut(s) 159
BshFI GGCC 1 cut(s) 255
BshNI GGYRCC 1 cut(s) 1054
BshVI ATCGAT 1 cut(s) 424
BslI CCNNNNNNNGG 2 cut(s) 121, 262
BsmAI GTCTC 4 cut(s) 546, 637, 748, 759
BsmI GAATGC 1 cut(s) 182
BsnI GGCC 1 cut(s) 255
Bso31I GGTCTC 2 cut(s) 748, 759
Bsp1286I GDGCHC 1 cut(s) 1057
Bsp143I GATC 3 cut(s) 78, 190, 506
Bsp1720I GCTNAGC 1 cut(s) 972
BspANI GGCC 1 cut(s) 255
BspCNI CTCAG 5 cut(s) 67, 112, 274, 597, 964
BspDI ATCGAT 1 cut(s) 424
BspLI GGNNCC 2 cut(s) 113, 1056
BspMAI CTGCAG 1 cut(s) 355
BspMI ACCTGC 1 cut(s) 442
BspPI GGATC 1 cut(s) 514
BspT107I GGYRCC 1 cut(s) 1054
BspTNI GGTCTC 2 cut(s) 748, 759
BsrDI GCAATG 1 cut(s) 1032
BsrI ACTGG 3 cut(s) 711, 801, 1058
BssECI CCNNGG 1 cut(s) 65
BssMI GATC 3 cut(s) 78, 190, 506
BssT1I CCWWGG 1 cut(s) 65
Bst2UI CCWGG 1 cut(s) 168
Bst4CI ACNGT 1 cut(s) 518
BstC8I GCNNGC 5 cut(s) 72, 253, 615, 939, 961
BstDEI CTNAG 5 cut(s) 75, 120, 261, 605, 972
BstF5I GGATG 3 cut(s) 486, 615, 626
BstKTI GATC 3 cut(s) 81, 193, 509
BstMAI GTCTC 4 cut(s) 546, 637, 748, 759
BstMBI GATC 3 cut(s) 78, 190, 506
BstMWI GCNNNNNNNGC 2 cut(s) 137, 257
BstNI CCWGG 1 cut(s) 168
BstNSI RCATGY 2 cut(s) 659, 985
BstSCI CCNGG 1 cut(s) 166
BstSFI CTRYAG 2 cut(s) 351, 850
BstSLI GKGCMC 1 cut(s) 1057
BstV1I GCAGC 2 cut(s) 335, 338
BstV2I GAAGAC 1 cut(s) 56
BstX2I RGATCY 1 cut(s) 78
BstYI RGATCY 1 cut(s) 78
Bsu15I ATCGAT 1 cut(s) 424
BsuRI GGCC 1 cut(s) 255
BsuTUI ATCGAT 1 cut(s) 424
BtgZI GCGATG 1 cut(s) 898
BtsCI GGATG 3 cut(s) 486, 615, 626
BtsI GCAGTG 1 cut(s) 360
BtsIMutI CAGTG 3 cut(s) 275, 360, 794
BveI ACCTGC 1 cut(s) 442
Cac8I GCNNGC 5 cut(s) 72, 253, 615, 939, 961
ClaI ATCGAT 1 cut(s) 424
Csp6I GTAC 2 cut(s) 39, 587
CviAII CATG 4 cut(s) 580, 656, 982, 1064
CviQI GTAC 2 cut(s) 39, 587
DdeI CTNAG 5 cut(s) 75, 120, 261, 605, 972
DpnI GATC 3 cut(s) 80, 192, 508
DpnII GATC 3 cut(s) 78, 190, 506
DraI TTTAAA 1 cut(s) 948
EaeI YGGCCR 1 cut(s) 253
Eco130I CCWWGG 1 cut(s) 65
Eco31I GGTCTC 2 cut(s) 748, 759
Eco57I CTGAAG 2 cut(s) 299, 924
EcoRI GAATTC 1 cut(s) 908
EcoRII CCWGG 1 cut(s) 166
EcoT14I CCWWGG 1 cut(s) 65
ErhI CCWWGG 1 cut(s) 65
FaeI CATG 4 cut(s) 583, 659, 985, 1067
FatI CATG 4 cut(s) 579, 655, 981, 1063
FbaI TGATCA 1 cut(s) 190
FblI GTMKAC 1 cut(s) 660
Fnu4HI GCNGC 2 cut(s) 324, 327
FokI GGATG 3 cut(s) 473, 622, 633
Fsp4HI GCNGC 2 cut(s) 324, 327
FspBI CTAG 3 cut(s) 132, 1002, 1019
GluI GCNGC 2 cut(s) 324, 327
GsaI CCCAGC 1 cut(s) 156
GsuI CTGGAG 2 cut(s) 728, 1059
HaeIII GGCC 1 cut(s) 255
Hin1II CATG 4 cut(s) 583, 659, 985, 1067
HincII GTYRAC 2 cut(s) 94, 661
HindII GTYRAC 2 cut(s) 94, 661
HindIII AAGCTT 1 cut(s) 281
HinfI GANTC 2 cut(s) 125, 1034
HphI GGTGA 1 cut(s) 200
Hpy166II GTNNAC 2 cut(s) 94, 661
Hpy188I TCNGA 1 cut(s) 121
Hpy188III TCNNGA 6 cut(s) 200, 311, 575, 992, 1019, 1038
Hpy8I GTNNAC 2 cut(s) 94, 661
HpyAV CCTTC 6 cut(s) 317, 497, 781, 889, 946, 989
HpyCH4III ACNGT 1 cut(s) 518
HpyCH4IV ACGT 1 cut(s) 650
HpyCH4V TGCA 5 cut(s) 140, 180, 215, 353, 599
HpyF10VI GCNNNNNNNGC 2 cut(s) 137, 257
HpyF3I CTNAG 5 cut(s) 75, 120, 261, 605, 972
HpySE526I ACGT 1 cut(s) 650
Hsp92II CATG 4 cut(s) 583, 659, 985, 1067
Ksp22I TGATCA 1 cut(s) 190
Kzo9I GATC 3 cut(s) 78, 190, 506
LmnI GCTCC 4 cut(s) 32, 111, 881, 956
Lsp1109I GCAGC 2 cut(s) 335, 338
LweI GCATC 2 cut(s) 495, 600
MaeI CTAG 3 cut(s) 132, 1002, 1019
MaeII ACGT 1 cut(s) 650
MaeIII GTNAC 3 cut(s) 651, 841, 898
MalI GATC 3 cut(s) 80, 192, 508
MboI GATC 3 cut(s) 78, 190, 506
MboII GAAGA 3 cut(s) 61, 310, 917
MflI RGATCY 1 cut(s) 78
MhlI GDGCHC 1 cut(s) 1057
MlsI TGGCCA 1 cut(s) 255
MluCI AATT 8 cut(s) 207, 225, 341, 418, 468, 887, 908, 1073
MluNI TGGCCA 1 cut(s) 255
Mox20I TGGCCA 1 cut(s) 255
MscI TGGCCA 1 cut(s) 255
MseI TTAA 4 cut(s) 99, 558, 924, 947
MslI CAYNNNNRTG 1 cut(s) 834
Msp20I TGGCCA 1 cut(s) 255
MspR9I CCNGG 1 cut(s) 168
Mva1269I GAATGC 1 cut(s) 182
MvaI CCWGG 1 cut(s) 168
MwoI GCNNNNNNNGC 2 cut(s) 137, 257
NdeII GATC 3 cut(s) 78, 190, 506
NlaIII CATG 4 cut(s) 583, 659, 985, 1067
NlaIV GGNNCC 2 cut(s) 113, 1056
NmuCI GTSAC 3 cut(s) 651, 841, 898
NspI RCATGY 2 cut(s) 659, 985
PciI ACATGT 1 cut(s) 655
PctI GAATGC 1 cut(s) 182
PfeI GAWTC 2 cut(s) 125, 1034
PfoI TCCNGGA 1 cut(s) 166
PkrI GCNGC 2 cut(s) 325, 328
PscI ACATGT 1 cut(s) 655
PshBI ATTAAT 1 cut(s) 924
PsiI TTATAA 1 cut(s) 366
Psp6I CCWGG 1 cut(s) 166
PspFI CCCAGC 1 cut(s) 152
PspGI CCWGG 1 cut(s) 166
PspN4I GGNNCC 2 cut(s) 113, 1056
PstI CTGCAG 1 cut(s) 355
PsuI RGATCY 1 cut(s) 78
RsaI GTAC 2 cut(s) 40, 588
RsaNI GTAC 2 cut(s) 39, 587
RseI CAYNNNNRTG 1 cut(s) 834
SalI GTCGAC 1 cut(s) 659
SaqAI TTAA 4 cut(s) 99, 558, 924, 947
SatI GCNGC 2 cut(s) 324, 327
Sau3AI GATC 3 cut(s) 78, 190, 506
ScrFI CCNGG 1 cut(s) 168
SduI GDGCHC 1 cut(s) 1057
SfaNI GCATC 2 cut(s) 495, 600
SfcI CTRYAG 2 cut(s) 351, 850
SmiMI CAYNNNNRTG 1 cut(s) 834
SmlI CTYRAG 3 cut(s) 200, 535, 748
SmoI CTYRAG 3 cut(s) 200, 535, 748
Sse9I AATT 8 cut(s) 207, 225, 341, 418, 468, 887, 908, 1073
SspMI CTAG 3 cut(s) 132, 1002, 1019
StyD4I CCNGG 1 cut(s) 166
StyI CCWWGG 1 cut(s) 65
TaaI ACNGT 1 cut(s) 518
TaiI ACGT 1 cut(s) 653
TaqI TCGA 2 cut(s) 424, 660
TasI AATT 8 cut(s) 207, 225, 341, 418, 468, 887, 908, 1073
TatI WGTACW 1 cut(s) 586
TfiI GAWTC 2 cut(s) 125, 1034
Tru1I TTAA 4 cut(s) 99, 558, 924, 947
Tru9I TTAA 4 cut(s) 99, 558, 924, 947
TscAI CASTG 3 cut(s) 282, 360, 801
TseFI GTSAC 3 cut(s) 651, 841, 898
TseI GCWGC 2 cut(s) 323, 326
Tsp45I GTSAC 3 cut(s) 651, 841, 898
TspDTI ATGAA 4 cut(s) 527, 559, 901, 981
TspRI CASTG 3 cut(s) 282, 360, 801
VspI ATTAAT 1 cut(s) 924
XapI RAATTY 2 cut(s) 908, 1073
XbaI TCTAGA 1 cut(s) 1018
XceI RCATGY 2 cut(s) 659, 985
XmiI GTMKAC 1 cut(s) 660
XspI CTAG 3 cut(s) 132, 1002, 1019
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.