Rorug05G0566400

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0002668
Physical Location & Seq
Forward (+)
75973099 .. 75981494
8396 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0566400.1

Sequence Viewer

Length: 7221 bp
ATGGACCCTGGAAAATCAATAGAGGATCAGTTCTCCAACCTGCATCCATGCCTGCCTCTGAACACAAGAATTGGGATAGTAGGCGCTGGTCCAAGTGGTTTGTCAGCTGCTTATGCTCTAGTAAAGCTCGGTTACAGCAATGTGACTGTGTTAGAGAAGTACCATTCTGTTGGTGGCATGTGTGAATCAGTCGATATTGAAGGGAACATTTATGATCTAGGTGGCCAAGTTCTTGCTGCAAACAGTGCCCCTGTCATTTTTCACTTGGCAAAGGAGACTGAGTCTGAACTAGAAGAAATGGACTCCCACAAGCTTGCTCTTATTGACAATGCTACAGGAGAGTATGAAGATATAAAAGTTGCAGATGACTATGTATCTGTGATCTCACTGACCTTAGAACTCCAGGATAAAGTAAAGAACTCAGGCCGAATTGGGATCCATGCTTTGAGTGAATTTGCCTCAGACTCGACTCCATCCTATCTTGAACGTCAAGGATTTAGTTCTGTTCCCAAATCTGTGGCTTATGGATATACTGCTTCTGGCTATGGGTATGTTGAAGACATGCCTTATGCTTACATTCATGAGTTCACCCGAACTTCTATGGCTGGAAAAATCCGCCGTTTTAAAGGTGGATATACAAGTCTTTGGAAGAAGATCAGTAAATCACTCCCAATGGTTCACTGCAATACTGAAGTATTGACAGTCAAACGTTTTTCTGATTATGTTAGTATCGATGTGAAAAAATGTGACGGAGAAGTCCAAAATATGGAGTTTGATAAGATCATTGTCTCTGGTTCATTTCCTTTGAACTATGGAAGAATTTACAGATCACCTTCACATTCTGCAGAACATAAAACGGAAGTGATGGATATGGGTGATGTTGAAAAGGAGTTGTTCAGTAAAGTACAGACCATTGACTACTACACAACTGTTTTGAAGGTTAAAGGGATAGAGCATATGCCTATAGGTTTCTATTACTTCAGTGAATACATAAGTAATCCTGCAACAATTGGACATCCAGTCGCAATGCAGAGATTCTTTGCTGACACAGATATTTTCTTATTCTGGTCTTATGGAAATTCTGCCGACATTATGGGACCAACTGTGACAGAACTTGCAATTGAAGCTGTTAAACGTATAGGGGGAGAAGTTAAGGAGGTGGTTCTCCAGCGCCGGTTTAAGTATTTCCCTCATGTTGATAGCCAAGCCATGATGAATGGATTTTATAACAAATTGGAGTCTGAACTTCAAGGCGTGAAGAACACTTATTACGTAGGCGGGCTTATGGCTTTTGAGCTTACAGAGAGAAATTCATCATATGCTATGGCTTTAGTCTGCAAGCACTTTGCAAATGACAATCCTTTGCCAAAGCTCCCATATGTTAAGAGTTTGTTTTCTTTCCAAAATGAATTGGAAAGAAGCCCTACAATCTTGGCCGAACTACCAGGAGTGGAGTACCCTAATCTGTCTTCCCTTGATGGCTATCTGAAGCACTGGGGAACTCATAGAGTCACTCAAAACAAGACACTCTATACTTGGATCAATGAAGAAGGGGCAGTTGCTTGCCAGAGGACTTACACAGAACTTAATGCAAATGCTTCTTGCATTGCTCAAAAGATCTTGACATGCCAGAAACCAGTAATCAAGCCTGGCGACAGGGTCCTCCTGGTCCATGTCCCGGGTTTGGACTTCATAGATGCTTTCTTTGGATGCTTAAGAGCTAAAGTCTTGCCAGTCCCAGTTCTACCTCCAGATCCTTTGCAAAGAGGTGGTCAGGCACTTTTGAAGATTGAAAACATTGCCAAATCATGTAATGCAAAGGCAATTCTGTCCACCATTAGTTATCACTGGGCAGTGCAAGCAGGTTCAGTTAAAAATATGATCTCATTGACTGCTAAGAATCAAAAGTCCTCGGCAAGGTGGCCGAATCTTCCTTGGTTACATACAGATTCTTGGGTTAAGAACTCCAAGAGCGGAGTTGTGGAAGCTCTAAAGGATGAATCTGAATCACAGCCAGGTGATGTATGTTTTCTCCAATTCACATCAGGTTCGACTGGCGATGCTAAAGGAGTCATGATAACTCATGGAGGGCTCATTCATAATGTCAAGTTGATGAGAAAGAGATACAAGAGCACCTCAAAAACAGTTCTAGTAAGCTGGCTTCCTCAGTACCACGACATGGGGCTGATCGGAGGACTTTTCACAGCTCTTGTTAGTGGTGGGACTGCAGTTTTGTTTTCACCATTGACATTCATCAGGAATCCACTATTATGGCTCCAAGTCATGAGCAAATATCAGGCTACTCACAGTGCTGGCCCCAACTTTGCCTTTGAGCTAGTAGTTAGAAGGCTAGAGTCTGACAAGACTAGAAAATATGACCTATCTTCCATGAAGTTCCTCATGGTTGCTGCTGAACCTGTTAGGCAGAAAACTCTGAAAAGGTTTGTTGATCTAACTCGTCCTTTCGGCTTAACTCAAGAGGTGATGGCACCTGGCTATGGCTTGGCAGAAAACTGTGTGTTTGTCAGTTGTGCATATGGTGAAGGAAAGCCTATCATGGTAGATTGGCAAGGAAGAGTATGTTGTGGATATGTGAATCCAAATGATGAAGATGTCGACATCAGAATAGTCGATCCAGAGAGCAGTGAGGAGCTCAAAGAAGCGGGAAAAGAAGGAGAAATATGGATCAGTAGTCCCAGTGCTGGAGTTGGGTATTGGGAAAGAGAAGAGCTAAGCAAGAAAACTTTTAAAAACCAGCTTGCTGACAATCCGAGACGTATCTACACTAGGACTGGGGATTTGGGACGCATAATTGACAGCAAACTCTTCATCACTGGAAGAATCAAAGATCTTATCATTGTTGCAGGAAGGAACATTTACTCAGCAGATGTAGAGAAGACAGTGGAGAATGCATCTGAAGTTGTACGTCCGGGTTGTTGTGCAGTCATTTCTGTTCCAGTGGAAATCCTATCAAAAAAAGGAATCTCAGTTCAAGACAACTCTGATCAGGTTGGTTTAGTTGTCATTGCGGAGGTTAGGGATGGAAAACCTGTTGGAAAGGATGTGGTTGAACAAATTCAAGCTCGTGTTGCAGAAGAACATGGGGTCACTGTTGCTACTATCAAGTTGATCAGGCCGAAAACCATTAGTAAAACAACGTCAGGAAAAATCAAAAGATTTGAATGCCTGCAGCAGTTTACTGATGGAACATTGAATATTGTACCAGAGCCACTTTTAACAAAGAGAAGGCTGATTCGGTCCTTCACTACAGGAACATGCAAGGAGGGAAAAACTCCTCGGCCACACCTTGTGAGAAGCACTCCTCCACCAAGCCCCAAAATAAGCAATAAACAGATTGTGGACTTCTTGAAACGATTAGTTTCCGAGCAGACTGGTATTTCAATCAACAAAATCACTGACACTGAAAGTCTAGTGTCTTATGGAATTGACTCCATTGGGGTGGTCAGAGCAGCTCAGAAACTTTCAGACTTCCTTGGTGTGCCAGTAGGAGCTGTGGACATTTTCACTGCAACCTGCATTGCAGACTTGGCAAGCTTCTCGGAGAATCTTTTAGTAAATGCTGAACCTCAAGTGTCCACTCAGCCTCACCTTGTAAGAAGCTCTCCTCCACCAAGCCTCAAAATAGGCAATAAACAGATTGTGGACTTCTTGAAACGGCTAGTTTCCGAGCAGACTGGTATTTCAATCAACAAAATCTCTGACACTGAAAGTTTAGTGTCTTATGGAATTGACTCCATTGGGGTGGTCAGAGCAGCTCAAAAACTTTCAGACTTTCTTGGTGTGCCAGTAGGAGCTGTGGACATTTTCACTGCAACCTGCATTGTCGACTTGGCAAGCTTCTCGGAGAATCTTTTAGTAAATGCTCAACCTCAACTGTCCACTGATCCATCCTATGTTGCACAACCTGAGACTGGTTCTGCTGAGCTTTCGATGGAACTTCCCGAGTCCAATCACTCGATTATCTGGTTATTCCAACTTCTAGCTCTTGTTTACGTTGCGTTCATGCTCTCTCTTCCTGCATATTTATCAGTTTCTACTTTCACAAGTTGTGTCTCAGCCACTCATGCATTGGTAGAAGGTGTTCCCTATCTAGATTATTTGGCCTTGCTGACCTTTGCTCCCCTTGCTTGGATGTTCTGCATATTTTCTACCTGTGTTTCAATTGCATTCTTAGGGAACTCTTTCTTAAAACCAAACTATGCCCTGAACCCTGAAGTCTCCGTCTGGTCAACGGATTTTGTCAAGTGGTGGGCTCTTTACAAGGGTCATGAAGTTGCCTCCAAAGTTTTGGCAGAGCCTCTGCGAGGCACGGTGTTCCTTAAGTATTGGTTTGAGATGCTTGGAGCTAGGATTGGATCCTCAGTTTTACTTGATACAGTTGACATAACAGACCCCTCTCTAGTTTCAATTGGAGATGGAGCTGTGATTGCAGAAGGTGCTTTGATTCAAGGCCATGAAGTGAAGAATGGAGTATTGAGCTTCCTCCCAATAAGAATTGGTCAAAACTCCTCAGTTGGCCCTTACGCTGTTGTCCAGAAGGGAACTATATTGGCAGAAGACAGTGAGCTGATGGCCTTACAAAAAGGTGGAGGCAAGTCTGTTATCAAGGCTAAAAATCATCATAATGGGCTGGCAAAGGTCACAAGCACTGAAACTGAGGCCATTTACCAGTTCTTAGGCATCTATTTGGTTGGGTTCCTCGGCACTCTCTCAGCTTCCATTGTGTATTTAGCCTACATGTGGCTGTCTCAAAAGCCTCCTTCGGTTGAAAAATATGCATTCTTTTGCTTATTTGGGGCCTTTCACTGGATACCTTACGCCATCATTGCTTATGCTACCATGTTTGCTAATGTTCCATCAGACATAATTTACTTCTCCATCTCAGTAGCTGTTGGTTACTTAGCTTATGGCCTAGTACTCAGCTTCCTCACAAGTGGTTTGACTCGTCTTATTTCTAGTAAACAGACAAAGTCAGGCCATCTCAGAACTTGGTTTTGCCACCGAATCACCATTGCTTGCCACCTTAGATTTGCTAAGCTTTTGTCAGGAACAGAAGCCTTCTGCATCTACTTGCGCCTTCTGGGTGCAAAGGTTGGAAAACATTGTTCTATCAGGGCCATCAATCCAGTTTCAGACCCGAAATTGTTATCACTTGGTTCTGGTGTCCATCTTGGTGATTTCAGTCGAATTATTGCTGGGTTTTATTCCTCCAATGGGTTTGTTAGCGGGAAAATTGAGGTGCAAGATAATGCGGTAATTGGGAGCGAAAGCGTAGTCCTTCCTGGCTCAGTTATTCAGAAAGATGTTATTCTTGGAGCACTTTCAGTTGCACCAGTGAATTCAATGCTTCAGGCTGGTGGTGTTTACATTGGTTCTCAAACTCCAATCATGATCAAGAACACCATGCATTCTTTGGATGACCGAATAGAAGAGATGGACATGAAATATAAGAAAATAGTTGGTAACCTTTCTGCAAATTTGGCTGCCACAACTCTGAAGGTCAAATCAAGATACTTCCATAGAATTGGTGTTAGTGGGAAGGGATACTTGAAGATTTATGACAACATCAAAGGGTTGCCGCACCATAACATTTTCTCTCCTGGCAAGAGCTATCCTGTTATAGTCCGGCACAGCAACAGCTTGAGTGCTGATGATGATGCAAGGATTGATGCACGCGGTGCAGCCATCCGAATACTTCAAGGTGAGACTAGTGAGTCCTCACTCCTTGACCTGACACTGAAGACAGGAAAGGCATTCTATGCCCGCACAATTGCAGACTTTGCAACATGGTTAGTGTGTGGACTTCCTGCAAGGGAAGAGTATGTCAAGCGAGCCCCACATGTACGCGATGCAGTGTGGACTTCCCTTCGCCATGCAAACTCGTTCACTGAGCTACATTACTACTCAAACATTTGCAGGTTGTTCCGGTTCACAGATGGACAAGAAATGTATGTCAAGTTCAAGTTGAGGCCTTCCAATGAGAACATTAGTGAAGAATCTGGTAAGGTAGAACCTATTGGCATTCTTCCACCTGATACAGGTGCAATTCCTAGAGATGAGAAGGATACTCGCCCTCTACTTTTCCTTGCCAAAGATTTCCAAAGCCGGGTCAATGAAACTGGAGTTCGTTATATATTCCAGTTACAGGTCAGGCCTGTTCCACATGATGAAGCTACCCGAGACATTGCACTTGACTGCACCAAACCCTGGAATGAGGCTGAGTTCCCATACATTGACGTTGGAGAGATTAACATCAACCACAATATATCTGCAGAAGAATCGGAACAGCTGAATTTCAATCCTTTTGTTAAATGCCAAGAAATAGATGTCATCAGGGCTTCATCATGCTCCCAGAGTGCTTCGATTGACCATGGCCGTTCACTGATCTATGAGATTTGCCAGCACTTACGCAATGGTGAACCCCTGCCAGAGGCTTGGAAAATCTTTGTAGAGCAGTCTGATGTCAAAGTAGACCTTTCTGGTTGTCCAATGGCAGCAGCATTGCAGAAAAAAGATTCACATACCGTGACGCTGGAAAGAACTGTCTTTCAGACTCTATGGGCAACTTTTGCGCAACCTTTTCTGCAAACAGTGTTCCCTCATTTCCTCCTCGCGTTAGTGATCTTCTATCCTTTGAGCTTGGCTCTTCACTTGAAGAATGTGCAGAAACTCCCTCTGCATTGGTTGTTCCCCTTGTTTTGGGTTTGTTCAGGATCTTTGGCTGGGTTAGCTTGTGTTGTTGCAAAATGGGTACTAGTAGGAAAGAAGAAAGAAGGTGAAGCCATACACATGTGGAGCATAGGGGTTTTTTTGGACACTACATGGCAAGCCTTTAGAACCCTAGTTGGGAGCTACTTCATGGAAATGACAAGCGGGTCAGTTCTCTTTGTGTTGTGGATGAGGCTGATGGGTTCAGAGATTGAGTTGGACCAAGGTGCTTATGTGGACAGCATGGGAGCTTTGCTCAACCCTGAAATGGTGGAGATTGAGAGAGGTGGGTGTGTGGGAAGAGAAGCTCTCTTGTTTGGTCACATTTATGAAGGGGATGGGGGCAAGGTTAAGTTCGGAAGGATTACAATTGAAGAAGGTGGATTTGTAGGGAGTAGAGCAATAGCAATGCCAGGAGTGATAGTGGAGAGTGGAGGCAGTCTTAGTGCTCTCTCTCTTGCCATGAAAGAAGAAATTATCAAGTCAAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000209 GO:0000902 GO:0000904 GO:0003006 GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006355 GO:0006357 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0006928 GO:0006935 GO:0007275 GO:0007399 GO:0007409 GO:0007411 GO:0007568 GO:0008045 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009605 GO:0009653 GO:0009791 GO:0009889 GO:0009966 GO:0009967 GO:0009987 GO:0010228 GO:0010259 GO:0010468 GO:0010498 GO:0010556 GO:0010646 GO:0010647 GO:0016043 GO:0016567 GO:0016740 GO:0019219 GO:0019222 GO:0019538 GO:0019787 GO:0019941 GO:0021675 GO:0022008 GO:0022414 GO:0023051 GO:0023056 GO:0030030 GO:0030154 GO:0030163 GO:0030182 GO:0030510 GO:0030513 GO:0031175 GO:0031323 GO:0031326 GO:0032446 GO:0032501 GO:0032502 GO:0032989 GO:0032990 GO:0032991 GO:0035107 GO:0035108 GO:0035136 GO:0036211 GO:0040011 GO:0042221 GO:0042330 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044464 GO:0048468 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048608 GO:0048666 GO:0048667 GO:0048699 GO:0048731 GO:0048736 GO:0048812 GO:0048856 GO:0048858 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0051603 GO:0060173 GO:0060255 GO:0060384 GO:0061061 GO:0061458 GO:0061564 GO:0061630 GO:0061659 GO:0065007 GO:0070647 GO:0071704 GO:0071840 GO:0080090 GO:0090092 GO:0090100 GO:0090287 GO:0097485 GO:0120036 GO:0120039 GO:0140096 GO:1901564 GO:1901565 GO:1901575 GO:1903506 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

2406

Amino Acids

265.35

Weight (kDa)

6.94

Isoelectric Point (pI)

37.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAD_binding_8 PF13450 26 - 92 5.9e-13 NAD(P)-binding Rossmann-like domain
Amino_oxidase PF01593 32 - 280 4.4e-15 Flavin containing amine oxidoreductase
AMP-binding PF00501 522 - 906 3e-59 AMP-binding enzyme
AMP-dom_DIP2-like PF23024 950 - 1073 1e-06 Disco-interacting protein 2-like, AMP domain
PP-binding PF00550 1122 - 1185 1.9e-10 Phosphopantetheine attachment site
PP-binding PF00550 1223 - 1286 7.3e-10 Phosphopantetheine attachment site
Catalase PF00199 1840 - 2111 3.3e-07 Catalase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1227
AasI GACNNNNNNGTC 3 cut(s) 755, 5734, 6895
Acc16I TGCGCA 1 cut(s) 6594
Acc36I ACCTGC 5 cut(s) 48, 1853, 3542, 3845, 5928
AccB1I GGYRCC 1 cut(s) 2488
AccB7I CCANNNNNTGG 3 cut(s) 766, 2179, 6228
AccBSI CCGCTC 1 cut(s) 1974
AccI GTMKAC 3 cut(s) 2616, 3846, 6492
AccII CGCG 3 cut(s) 5697, 5868, 6635
AclI AACGTT 1 cut(s) 709
AcoI YGGCCR 5 cut(s) 223, 1434, 1922, 3299, 6394
AcsI RAATTY 8 cut(s) 452, 819, 1078, 1309, 3075, 5359, 5497, 6313
AcuI CTGAAG 8 cut(s) 711, 964, 1508, 2937, 4254, 5354, 5537, 5780
AdeI CACNNNGTG 2 cut(s) 3310, 5699
AfaI GTAC 9 cut(s) 161, 906, 1457, 2171, 2925, 3222, 4938, 5865, 6774
AflII CTTAAG 2 cut(s) 1714, 4340
AflIII ACRYGT 3 cut(s) 4758, 5860, 6810
AhdI GACNNNNNGTC 1 cut(s) 1019
AhlI ACTAGT 2 cut(s) 5729, 6775
AloI GAACNNNNNNTCC 2 cut(s) 2973, 3005
Alw21I GWGCWC 4 cut(s) 2135, 2655, 5341, 7180
Alw26I GTCTC 9 cut(s) 269, 793, 2767, 3923, 4078, 4243, 4773, 5720, 6195
AlwNI CAGNNNCTG 1 cut(s) 4910
Ama87I CYCGRG 3 cut(s) 1678, 3962, 6198
ApoI RAATTY 8 cut(s) 452, 819, 1078, 1309, 3075, 5359, 5497, 6313
ArsI GACNNNNNNTTYG 8 cut(s) 1756, 1788, 2215, 2247, 2782, 2814, 5280, 5312
Asp700I GAANNNNTTC 3 cut(s) 3075, 4101, 4202
AspLEI GCGC 4 cut(s) 86, 1173, 5097, 6595
AsuC2I CCSGG 4 cut(s) 1679, 1680, 2931, 6128
AvaI CYCGRG 3 cut(s) 1678, 3962, 6198
AvaII GGWCC 7 cut(s) 4, 89, 1097, 1660, 1669, 3258, 6949
BaeGI GKGCMC 1 cut(s) 250
BalI TGGCCA 1 cut(s) 225
BamHI GGATCC 2 cut(s) 435, 4376
BanI GGYRCC 1 cut(s) 2488
BanII GRGCYC 4 cut(s) 2094, 2655, 4276, 5857
BarI GAAGNNNNNNTAC 2 cut(s) 2145, 2177
BauI CACGAG 1 cut(s) 3084
BbsI GAAGAC 5 cut(s) 564, 1461, 2903, 4584, 5768
Bbv12I GWGCWC 4 cut(s) 2135, 2655, 5341, 7180
BceAI ACGGC 3 cut(s) 603, 3692, 6381
BcgI CGANNNNNNTGC 6 cut(s) 3540, 3574, 3843, 3877, 6282, 6316
BciVI GTATCC 3 cut(s) 4824, 5558, 6079
BclI TGATCA 3 cut(s) 3004, 3129, 5412
BcnI CCSGG 4 cut(s) 1679, 1680, 2931, 6128
BcoDI GTCTC 9 cut(s) 269, 793, 2767, 3923, 4078, 4243, 4773, 5720, 6195
BcuI ACTAGT 2 cut(s) 5729, 6775
BfmI CTRYAG 7 cut(s) 333, 843, 963, 2226, 3188, 3267, 6291
BfoI RGCGCY 2 cut(s) 87, 1174
BfrI CTTAAG 2 cut(s) 1714, 4340
BfuAI ACCTGC 5 cut(s) 48, 1853, 3542, 3845, 5928
BfuI GTATCC 3 cut(s) 4824, 5558, 6079
BglII AGATCT 2 cut(s) 1617, 2848
BlpI GCTNAGC 3 cut(s) 2732, 3942, 5055
BmcAI AGTACT 1 cut(s) 4938
Bme18I GGWCC 7 cut(s) 4, 89, 1097, 1660, 1669, 3258, 6949
BmeRI GACNNNNNGTC 1 cut(s) 1019
BmeT110I CYCGRG 3 cut(s) 1678, 3962, 6198
BmrI ACTGGG 5 cut(s) 1504, 1733, 1858, 2691, 2802
BmuI ACTGGG 5 cut(s) 1504, 1733, 1858, 2691, 2802
BpiI GAAGAC 5 cut(s) 564, 1461, 2903, 4584, 5768
BplI GAGNNNNNCTC 8 cut(s) 3304, 3336, 6649, 6681, 6969, 7001, 7023, 7055
BpmI CTGGAG 5 cut(s) 386, 1151, 1734, 2724, 6162
Bpu1102I GCTNAGC 3 cut(s) 2732, 3942, 5055
BpuEI CTTGAG 3 cut(s) 2460, 3573, 5683
BpuMI CCSGG 4 cut(s) 1679, 1680, 2931, 6128
Bsa29I ATCGAT 1 cut(s) 732
BsaAI YACGTR 1 cut(s) 1273
BsaBI GATNNNNATC 2 cut(s) 1482, 6272
BsaWI WCCGGW 1 cut(s) 5946
BsaXI ACNNNNNCTCC 6 cut(s) 266, 296, 4123, 4153, 7137, 7167
Bse118I RCCGGY 1 cut(s) 1173
Bse8I GATNNNNATC 2 cut(s) 1482, 6272
BseCI ATCGAT 1 cut(s) 732
BseJI GATNNNNATC 2 cut(s) 1482, 6272
BseRI GAGGAG 6 cut(s) 2663, 3285, 3312, 3615, 4519, 6620
BseSI GKGCMC 1 cut(s) 250
BseYI CCCAGC 2 cut(s) 5216, 6743
BsgI GTGCAG 4 cut(s) 2961, 5721, 6202, 6704
Bsh1236I CGCG 3 cut(s) 5697, 5868, 6635
BshNI GGYRCC 1 cut(s) 2488
BshVI ATCGAT 1 cut(s) 732
BsiHKAI GWGCWC 4 cut(s) 2135, 2655, 5341, 7180
BsiHKCI CYCGRG 3 cut(s) 1678, 3962, 6198
BsiSI CCGG 6 cut(s) 1174, 1679, 2930, 5647, 5947, 6127
BslFI GGGAC 6 cut(s) 1110, 1661, 1721, 2236, 2679, 2817
BsmAI GTCTC 9 cut(s) 269, 793, 2767, 3923, 4078, 4243, 4773, 5720, 6195
BsmBI CGTCTC 1 cut(s) 2767
BsmFI GGGAC 6 cut(s) 1110, 1661, 1721, 2236, 2679, 2817
BsmI GAATGC 7 cut(s) 2914, 3188, 4187, 4799, 5428, 5774, 6042
BsoBI CYCGRG 3 cut(s) 1678, 3962, 6198
Bsp1286I GDGCHC 8 cut(s) 250, 2094, 2135, 2655, 4276, 5341, 5857, 7180
Bsp1720I GCTNAGC 3 cut(s) 2732, 3942, 5055
Bsp19I CCATGG 1 cut(s) 6391
BspDI ATCGAT 1 cut(s) 732
BspFNI CGCG 3 cut(s) 5697, 5868, 6635
BspHI TCATGA 5 cut(s) 580, 2073, 2283, 4288, 5409
BspMAI CTGCAG 4 cut(s) 847, 2230, 3192, 6295
BspMI ACCTGC 5 cut(s) 48, 1853, 3542, 3845, 5928
BspQI GCTCTTC 2 cut(s) 2721, 6672
BspT107I GGYRCC 1 cut(s) 2488
BspTI CTTAAG 2 cut(s) 1714, 4340
BsrBI CCGCTC 1 cut(s) 1974
BsrFI RCCGGY 1 cut(s) 1173
BssAI RCCGGY 1 cut(s) 1173
BssSI CACGAG 1 cut(s) 3084
BssT1I CCWWGG 4 cut(s) 1934, 3493, 6391, 6952
Bst2BI CACGAG 1 cut(s) 3084
Bst6I CTCTTC 8 cut(s) 2569, 2721, 2831, 4038, 5445, 5832, 6672, 7024
BstAFI CTTAAG 2 cut(s) 1714, 4340
BstAPI GCANNNNNTGC 6 cut(s) 245, 4457, 5699, 5780, 5801, 6590
BstBAI YACGTR 1 cut(s) 1273
BstDSI CCRYGG 1 cut(s) 6391
BstEII GGTNACC 1 cut(s) 5483
BstENI CCTNNNNNAGG 4 cut(s) 1915, 4323, 6057, 6449
BstFNI CGCG 3 cut(s) 5697, 5868, 6635
BstH2I RGCGCY 2 cut(s) 87, 1174
BstHHI GCGC 4 cut(s) 86, 1173, 5097, 6595
BstMAI GTCTC 9 cut(s) 269, 793, 2767, 3923, 4078, 4243, 4773, 5720, 6195
BstNSI RCATGY 7 cut(s) 181, 565, 1629, 3279, 4762, 5864, 6814
BstPI GGTNACC 1 cut(s) 5483
BstSFI CTRYAG 7 cut(s) 333, 843, 963, 2226, 3188, 3267, 6291
BstSLI GKGCMC 1 cut(s) 250
BstSNI TACGTA 1 cut(s) 1273
BstUI CGCG 3 cut(s) 5697, 5868, 6635
BstV2I GAAGAC 5 cut(s) 564, 1461, 2903, 4584, 5768
BstX2I RGATCY 6 cut(s) 435, 1617, 1753, 2848, 4376, 6734
BstXI CCANNNNNNTGG 8 cut(s) 170, 517, 2271, 3460, 3763, 4309, 5546, 6456
BstYI RGATCY 6 cut(s) 435, 1617, 1753, 2848, 4376, 6734
Bsu15I ATCGAT 1 cut(s) 732
BsuI GTATCC 3 cut(s) 4824, 5558, 6079
BsuTUI ATCGAT 1 cut(s) 732
BtgI CCRYGG 1 cut(s) 6391
BtgZI GCGATG 2 cut(s) 2073, 5883
BtsI GCAGTG 6 cut(s) 679, 1860, 2650, 3525, 3828, 5880
BveI ACCTGC 5 cut(s) 48, 1853, 3542, 3845, 5928
CaiI CAGNNNCTG 1 cut(s) 4910
CciI TCATGA 5 cut(s) 580, 2073, 2283, 4288, 5409
CfoI GCGC 4 cut(s) 86, 1173, 5097, 6595
Cfr10I RCCGGY 1 cut(s) 1173
Cfr9I CCCGGG 1 cut(s) 1678
ClaI ATCGAT 1 cut(s) 732
CseI GACGC 2 cut(s) 2814, 6559
Csp6I GTAC 9 cut(s) 160, 905, 1456, 2170, 2924, 3221, 4937, 5864, 6773
CviQI GTAC 9 cut(s) 160, 905, 1456, 2170, 2924, 3221, 4937, 5864, 6773
DraI TTTAAA 2 cut(s) 625, 2749
DraIII CACNNNGTG 2 cut(s) 3310, 5699
DrdI GACNNNNNNGTC 3 cut(s) 755, 5734, 6895
DriI GACNNNNNGTC 1 cut(s) 1019
DseDI GACNNNNNNGTC 3 cut(s) 755, 5734, 6895
EaeI YGGCCR 5 cut(s) 223, 1434, 1922, 3299, 6394
Eam1104I CTCTTC 8 cut(s) 2569, 2721, 2831, 4038, 5445, 5832, 6672, 7024
Eam1105I GACNNNNNGTC 1 cut(s) 1019
EarI CTCTTC 8 cut(s) 2569, 2721, 2831, 4038, 5445, 5832, 6672, 7024
EciI GGCGGA 1 cut(s) 605
Ecl136II GAGCTC 1 cut(s) 2653
Eco105I TACGTA 1 cut(s) 1273
Eco130I CCWWGG 4 cut(s) 1934, 3493, 6391, 6952
Eco147I AGGCCT 2 cut(s) 5992, 6175
Eco24I GRGCYC 4 cut(s) 2094, 2655, 4276, 5857
Eco47I GGWCC 7 cut(s) 4, 89, 1097, 1660, 1669, 3258, 6949
Eco53kI GAGCTC 1 cut(s) 2653
Eco57I CTGAAG 8 cut(s) 711, 964, 1508, 2937, 4254, 5354, 5537, 5780
Eco88I CYCGRG 3 cut(s) 1678, 3962, 6198
Eco91I GGTNACC 1 cut(s) 5483
EcoICRI GAGCTC 1 cut(s) 2653
EcoNI CCTNNNNNAGG 4 cut(s) 1915, 4323, 6057, 6449
EcoO109I RGGNCCY 2 cut(s) 1660, 4818
EcoO65I GGTNACC 1 cut(s) 5483
EcoRI GAATTC 1 cut(s) 5359
EcoT14I CCWWGG 4 cut(s) 1934, 3493, 6391, 6952
EcoT22I ATGCAT 4 cut(s) 2914, 4090, 4801, 5430
EcoT38I GRGCYC 4 cut(s) 2094, 2655, 4276, 5857
ErhI CCWWGG 4 cut(s) 1934, 3493, 6391, 6952
Esp3I CGTCTC 1 cut(s) 2767
FalI AAGNNNNNCTT 4 cut(s) 5552, 5584, 6480, 6512
FaqI GGGAC 6 cut(s) 1110, 1661, 1721, 2236, 2679, 2817
FauI CCCGC 5 cut(s) 1271, 2656, 5240, 5792, 6887
FauNDI CATATG 4 cut(s) 957, 1318, 1378, 2536
FbaI TGATCA 3 cut(s) 3004, 3129, 5412
FblI GTMKAC 3 cut(s) 2616, 3846, 6492
FriOI GRGCYC 4 cut(s) 2094, 2655, 4276, 5857
FspI TGCGCA 1 cut(s) 6594
GlaI GCGC 4 cut(s) 85, 1172, 5096, 6594
GsaI CCCAGC 2 cut(s) 5220, 6747
GsuI CTGGAG 5 cut(s) 386, 1151, 1734, 2724, 6162
HaeII RGCGCY 2 cut(s) 87, 1174
HapII CCGG 6 cut(s) 1174, 1679, 2930, 5647, 5947, 6127
HgaI GACGC 2 cut(s) 2814, 6559
HhaI GCGC 4 cut(s) 86, 1173, 5097, 6595
Hin6I GCGC 4 cut(s) 84, 1171, 5095, 6593
HinP1I GCGC 4 cut(s) 84, 1171, 5095, 6593
HincII GTYRAC 4 cut(s) 2617, 3847, 4251, 4402
HindII GTYRAC 4 cut(s) 2617, 3847, 4251, 4402
HindIII AAGCTT 4 cut(s) 311, 3553, 3856, 5057
HpaII CCGG 6 cut(s) 1174, 1679, 2930, 5647, 5947, 6127
HpyCH4IV ACGT 9 cut(s) 487, 709, 1135, 1272, 2776, 2926, 3158, 4014, 6258
HpySE526I ACGT 9 cut(s) 487, 709, 1135, 1272, 2776, 2926, 3158, 4014, 6258
HspAI GCGC 4 cut(s) 84, 1171, 5095, 6593
Ksp22I TGATCA 3 cut(s) 3004, 3129, 5412
LguI GCTCTTC 2 cut(s) 2721, 6672
MaeII ACGT 9 cut(s) 487, 709, 1135, 1272, 2776, 2926, 3158, 4014, 6258
MbiI CCGCTC 1 cut(s) 1974
MfeI CAATTG 6 cut(s) 1008, 1119, 4182, 4428, 5790, 7098
MflI RGATCY 6 cut(s) 435, 1617, 1753, 2848, 4376, 6734
MhlI GDGCHC 8 cut(s) 250, 2094, 2135, 2655, 4276, 5341, 5857, 7180
MlsI TGGCCA 1 cut(s) 225
MluNI TGGCCA 1 cut(s) 225
MmeI TCCRAC 6 cut(s) 60, 3034, 4018, 5095, 6241, 6927
Mox20I TGGCCA 1 cut(s) 225
Mph1103I ATGCAT 4 cut(s) 2914, 4090, 4801, 5430
MroXI GAANNNNTTC 3 cut(s) 3075, 4101, 4202
MscI TGGCCA 1 cut(s) 225
MslI CAYNNNNRTG 8 cut(s) 2269, 3458, 3761, 4644, 5193, 6809, 6884, 7056
Msp20I TGGCCA 1 cut(s) 225
MspA1I CMGCKG 2 cut(s) 107, 6310
MspCI CTTAAG 2 cut(s) 1714, 4340
MspI CCGG 6 cut(s) 1174, 1679, 2930, 5647, 5947, 6127
MunI CAATTG 6 cut(s) 1008, 1119, 4182, 4428, 5790, 7098
Mva1269I GAATGC 7 cut(s) 2914, 3188, 4187, 4799, 5428, 5774, 6042
MvnI CGCG 3 cut(s) 5697, 5868, 6635
NciI CCSGG 4 cut(s) 1679, 1680, 2931, 6128
NcoI CCATGG 1 cut(s) 6391
NdeI CATATG 4 cut(s) 957, 1318, 1378, 2536
NmeAIII GCCGAG 3 cut(s) 1892, 3277, 4701
NmuCI GTSAC 8 cut(s) 142, 746, 1105, 1510, 3106, 4660, 6547, 7049
NsbI TGCGCA 1 cut(s) 6594
NsiI ATGCAT 4 cut(s) 2914, 4090, 4801, 5430
NspI RCATGY 7 cut(s) 181, 565, 1629, 3279, 4762, 5864, 6814
PagI TCATGA 5 cut(s) 580, 2073, 2283, 4288, 5409
PceI AGGCCT 2 cut(s) 5992, 6175
PciI ACATGT 3 cut(s) 4758, 5860, 6810
PciSI GCTCTTC 2 cut(s) 2721, 6672
PctI GAATGC 7 cut(s) 2914, 3188, 4187, 4799, 5428, 5774, 6042
PdmI GAANNNNTTC 3 cut(s) 3075, 4101, 4202
PflFI GACNNNGTC 3 cut(s) 280, 1658, 4990
PflMI CCANNNNNTGG 3 cut(s) 766, 2179, 6228
PfoI TCCNGGA 1 cut(s) 402
Ppu21I YACGTR 1 cut(s) 1273
PpuMI RGGWCCY 1 cut(s) 1660
PscI ACATGT 3 cut(s) 4758, 5860, 6810
PsiI TTATAA 1 cut(s) 1227
Psp124BI GAGCTC 1 cut(s) 2655
Psp1406I AACGTT 1 cut(s) 709
Psp5II RGGWCCY 1 cut(s) 1660
PspEI GGTNACC 1 cut(s) 5483
PspFI CCCAGC 2 cut(s) 5216, 6743
PspPPI RGGWCCY 1 cut(s) 1660
PsrI GAACNNNNNNTAC 6 cut(s) 1253, 1285, 4086, 4118, 5963, 5995
PstI CTGCAG 4 cut(s) 847, 2230, 3192, 6295
PstNI CAGNNNCTG 1 cut(s) 4910
PsuI RGATCY 6 cut(s) 435, 1617, 1753, 2848, 4376, 6734
PsyI GACNNNGTC 3 cut(s) 280, 1658, 4990
PvuII CAGCTG 2 cut(s) 107, 6310
RsaI GTAC 9 cut(s) 161, 906, 1457, 2171, 2925, 3222, 4938, 5865, 6774
RsaNI GTAC 9 cut(s) 160, 905, 1456, 2170, 2924, 3221, 4937, 5864, 6773
RseI CAYNNNNRTG 8 cut(s) 2269, 3458, 3761, 4644, 5193, 6809, 6884, 7056
SacI GAGCTC 1 cut(s) 2655
SalI GTCGAC 2 cut(s) 2615, 3845
SapI GCTCTTC 2 cut(s) 2721, 6672
ScaI AGTACT 1 cut(s) 4938
SduI GDGCHC 8 cut(s) 250, 2094, 2135, 2655, 4276, 5341, 5857, 7180
SfcI CTRYAG 7 cut(s) 333, 843, 963, 2226, 3188, 3267, 6291
SinI GGWCC 7 cut(s) 4, 89, 1097, 1660, 1669, 3258, 6949
SmaI CCCGGG 1 cut(s) 1680
SmiMI CAYNNNNRTG 8 cut(s) 2269, 3458, 3761, 4644, 5193, 6809, 6884, 7056
SmlI CTYRAG 5 cut(s) 1714, 2475, 3588, 4340, 5662
SmoI CTYRAG 5 cut(s) 1714, 2475, 3588, 4340, 5662
SnaBI TACGTA 1 cut(s) 1273
SpeI ACTAGT 2 cut(s) 5729, 6775
SseBI AGGCCT 2 cut(s) 5992, 6175
SspI AATATT 1 cut(s) 3217
SstI GAGCTC 1 cut(s) 2655
StuI AGGCCT 2 cut(s) 5992, 6175
StyI CCWWGG 4 cut(s) 1934, 3493, 6391, 6952
TaiI ACGT 9 cut(s) 490, 712, 1138, 1275, 2779, 2929, 3161, 4017, 6261
TaqII GACCGA 2 cut(s) 3246, 5457
TatI WGTACW 2 cut(s) 904, 4936
TauI GCSGC 1 cut(s) 5602
TseFI GTSAC 8 cut(s) 142, 746, 1105, 1510, 3106, 4660, 6547, 7049
Tsp45I GTSAC 8 cut(s) 142, 746, 1105, 1510, 3106, 4660, 6547, 7049
TspGWI ACGGA 4 cut(s) 765, 872, 4231, 4268
TspMI CCCGGG 1 cut(s) 1678
Tth111I GACNNNGTC 3 cut(s) 280, 1658, 4990
Van91I CCANNNNNTGG 3 cut(s) 766, 2179, 6228
Vha464I CTTAAG 2 cut(s) 1714, 4340
VpaK11BI GGWCC 7 cut(s) 4, 89, 1097, 1660, 1669, 3258, 6949
XagI CCTNNNNNAGG 4 cut(s) 1915, 4323, 6057, 6449
XapI RAATTY 8 cut(s) 452, 819, 1078, 1309, 3075, 5359, 5497, 6313
XbaI TCTAGA 1 cut(s) 4111
XceI RCATGY 7 cut(s) 181, 565, 1629, 3279, 4762, 5864, 6814
XcmI CCANNNNNNNNNTGG 3 cut(s) 170, 4087, 5431
XmaI CCCGGG 1 cut(s) 1678
XmiI GTMKAC 3 cut(s) 2616, 3846, 6492
XmnI GAANNNNTTC 3 cut(s) 3075, 4101, 4202
ZrmI AGTACT 1 cut(s) 4938
Zsp2I ATGCAT 4 cut(s) 2914, 4090, 4801, 5430
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.